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Готові списки джерел за темами / Semen

Добірка наукової літератури з теми "Semen"

Автор: Grafiati

Опубліковано: 4 червня 2021

Оновлено: 10 березня 2023

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Статті в журналах з теми "Semen":

1

Budai, Csilla, István Egerszegi, József Rátky, and András Kovács. "Storage of ram semen in gelatin supplemented extender." Acta Agraria Debreceniensis, no. 48 (July 31, 2012): 7–10. http://dx.doi.org/10.34101/actaagrar/48/2444.

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The aim of our study was to examine how different gelatin concentrations affect ram semens viability in liquid storage at 5 oC for five days. Our hypothesis was if we add gelatin to the semen extender, than the viability of ram semen will be better in the extenders containing gelatin, than the control. We used two different semen extenders:1.5% UHT milk and 1.5% UHT milk + 5% egg yolk. We added 0; 0.5; 1.0; 1.5; 2.0% Dr. Oetker gelatin to the semen extenders. We stored the semen for five days at 5 oC and in every 24 hour we made sampling.We stained the smears with Kovács-Foote staining and evaluated them with light-microscope. We categorized the cells in five groups like: live and intact cells, live cells with injured acrosome, dead cells, live head with dead tail and live tail with dead head. We used one-way analysis of variance (ANOVA) to assign how gelatin concentration affects the number of the categorized cells. On the fifth day, the viability was the best in the following semen extenders: 1.5% fat UHT milk + 1.0% gelatin and 1.5% fat UHT milk + 1.5% gelatin, but it was not significant (p>0.05).

2

Akbar Irawan and Mohammad Azhar. "ANALISIS PENGGUNAAN SLAG UNTUK MEREDUKSI SEMEN PADA CAMPURAN BETON." Jurnal Gradasi Teknik Sipil 6, no. 2 (December 30, 2022): 142–49. http://dx.doi.org/10.31961/gradasi.v6i2.1471.

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Penelitian penggunaan slag semen pada campuran beton sebagai cementious telah dimulai sejak tahun 1774. Saat ini di negara-negara maju slag semen telah dimanfaatkan pada setiap campuran beton, bahkan persentasenya mencapai 30% dari cementious, hal ini dapat dilihat dari produksi slag semen yang terus meningkat dari tahun ke tahun. Pembuatan beton dengan menggunakan slag sement sebagai campuran agregat halus untuk subtitusi terhadap semen yang selanjutnya dilakukan pengujian terhadap benda uji beton untuk mengetahui seberapa besar pengaruhnya terhadap nilai kuat tekan pada beton, serta bertujuan juga untuk mendapatkan campuran bahan beton dengan biaya yang lebih efisien dibandingkan beton non-slag. Kuat tekan Beton akan mengidentifikasi mutu dari sebuah konstruksi, semakin tinggi kekuatan struktur yang direncanakan , maka semakin tinggi pula mutu beton yang harus di hasilkan. Beberapa faktor yang mempengaruhi kuat tekan beton, yaitu faktor air semen (w/c), jumlah air, jumlah dan kualitas semen, kualitas agregat, umur beton, serta perawatan beton. Apabila w/c tidak sesuai dengan beton yang direncanakan maka beton akan mengalami perubahan kekuatan. Hubungan antara kuat tekan beton dengan nilai w/c berbanding terbalik, kuat tekan akan menjadi tinggi apabila angka w/c kecil dan sebaliknya kuat tekan akan rendah apabila w/c makin besar. Hasil penelitian ini menunjukan bahwa perbandingan komposisi untuk memperoleh mutu yang sama didapatkan hasil pada beton slag ada penambahan air 7 liter/m³ terhadap non-slag, dan mengurangi sementious sebesar 2 kg/m³, serta slag semen memberikan kontribusi terhadap kuat tekan lebih besar dari pada beton non-slag sebesar 4,3%, akan tetapi dalam mencapai hal itu, slag semen membutuhkan air yang lebih banyak, sedangkan dari segi biaya, dibandingkan beton non-slag, slag semen dapat menekan biaya sebesar Rp 15.354,00/m3.

3

La Vignera, Sandro, Rosita A. Condorelli, Enzo Vicari, Dario Tumino, Giuseppe Morgia, Vincenzo Favilla, Sebastiano Cimino, and Aldo E. Calogero. "Markers of semen inflammation: supplementary semen analysis?" Journal of Reproductive Immunology 100, no. 1 (November 2013): 2–10. http://dx.doi.org/10.1016/j.jri.2013.05.001.

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4

Lee, Seungho, Youngsik Kim, Junghoon Kim, Guemsan Lee, Goya Choi, and Youngsung Ju. "Identification of Armeniacae Semen and Persicae Semen." Korea Journal of Herbology 29, no. 4 (July 30, 2014): 61–67. http://dx.doi.org/10.6116/kjh.2014.29.4.61.

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5

Kim, Young-Sik, and Young-Sung Ju. "Identification of Morphological Appearance of Minute Seed Herbs Using Stereoscope (Report II) - Alli Tuberosi Semen✳Alli Fistulosi Semen, Malvae Semen✳Abutili Semen, Plantaginis Semen✳Schizonepetae Semen." Korea Journal of Herbology 31, no. 4 (July 30, 2016): 61–69. http://dx.doi.org/10.6116/kjh.2016.31.4.61.

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6

Jovičić, Marija, Eva Chmelíková, and Markéta Sedmíková. "Cryopreservation of boar semen." Czech Journal of Animal Science 65, No. 4 (April 30, 2020): 115–23. http://dx.doi.org/10.17221/47/2020-cjas.

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Sperm cryopreservation is the best technology for long-term storage of the semen. However, the damage of boar spermatozoa by cryopreservation is more severe than in other animal species and a standardized freezing protocol for efficient cryopreservation has not been established yet. Semen quality and freezability vary greatly between breeds as well as between individual boars and even the season. Boar spermatozoa are sensitive to low temperatures; they sustain damage and a high rate of mortality and freezing/thawing the boar semen may strongly impair the sperm function and decrease the semen quality. The freezability of boar semen can be influenced by a cryopreservation procedure, and also by using various additives to freezing and thawing extenders such as antioxidants. In order to obtain acceptable results after thawing the boar semen, it is necessary to combine an optimal amount of additives (glycerol, egg yolk, sugars, antioxidants), cooling and warming velocities.

7

Dasrul, Dasrul, Sri Wahyuni, Sugito Sugito, Abdulah Hamzah, Zulzya Zaini, Abdul Haris, and Gholib Gholib. "Correlation Between Testosterone Concentrations with Scrotal Circumference, and Semen Characteristics in Aceh Bulls." E3S Web of Conferences 151 (2020): 01015. http://dx.doi.org/10.1051/e3sconf/202015101015.

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This study was conducted to examine testosterone concentrations its relationship with the scrotal circumference and physical characteristics of semen in aceh bulls. Semen samples were collected weekly from jugular vein of three aceh bulls aged 4-5 years old for 10 weeks. Testosterone concentration was measured by enzyme-linked immunosorbent assay (ELISA) method. Semens were collected by using artificial vagina and evaluated for physical characteristics namely ejaculatory volume, pH, and sperm motility, concentration, and abnormalities. Data were analyzed using correlation-regresion test. Testosterone concentrations showed a positive correlation with scrotal circumference (r = 0.799), number of sperm (r = 0.703), sperm motility (r = 0.857) and sperm abnormalities (r = -0,877). No correlation, however, was found between testosterone concentrations with semen volume (r = 0.038) and pH (r = 0.418). It can be concluded that testosterone concentrations correlated positively with scrotal circumference, numbers of sperm, sperm of motility and sperm of abnormality.

8

Sultan, Jahangir, Shafqat Husnain Khan, and Munir Ahmed. "SEMEN PARAMETERS;." Professional Medical Journal 25, no. 07 (July 11, 2018): 1024–28. http://dx.doi.org/10.29309/tpmj/18.4478.

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9

Khan, Haroon Latif, Yousaf Latif Khan, Irfan Mehfooz, Muhammad Burhan, Saba Sardar, and Abdul Rahman Khawaja. "SEMEN PARAMETERS." Professional Medical Journal 23, no. 05 (May 10, 2016): 589–96. http://dx.doi.org/10.29309/tpmj/2016.23.05.1589.

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Semen is a pale whitish fluid secreted by male during ejaculation and containsspermatozoa which are male gametes essential of fertilizing the oocytes which are femalegametes. In a quest to evaluate male’s fertility potential semen is analyzed to look into some ofits characteristics and of the sperms contained within the semen analyzed. Method of collectioninfluences the results of Semen analysis as does the technique of analysis. Spermatozoa areexamined for number (count), shape (morphology) and movement (motility) in order to assesstheir quality. Non sperm cells, volume, Fructose level, pH, liquefaction are also checked asa part of routine analysis. Objectives: To describe the pattern of semen parameters in subfertilemales. To look into frequency and distribution of abnormal semen parameters in a groupof Pakistani males in Lahore. Methods: In this Retrospective, cross sectional, observationalstudy all males undergoing for evaluation and treatment for sub-fertility at a private AssistedReproductive Technology clinic in Lahore, Pakistan were included. Approval of the IRB wassought and data collection instrument was a specially designed Performa which was validatedby the biostatistician of LIFE research cell. Data was extracted from the files of LIFE (LahoreInstitute of Fertility and Endocrinology) and entered in SPSS version 15. Sampling techniquewas non-probability, consecutive. Semen analysis was done by methods defined by the WHO(World Health Organization). Results: Of total patient (n=679) 92.2% (626) males passed sampleat LIFE (Lahore institute of fertility and endocrinology) and (7.8%) 53 brought sample fromhome. Of the males who passed sample at LIFE (78.8%) 535 collected semen by masturbation,(11.9%) 81 by coitus; the source of sample of (9.3%) 63 males was not known. As 2-6 ml semenwas consider to be normal by WHO criteria, (80.6%) 547 males were in normal range (14.1%)96 found to be less than 2-6 ml and (5.3%) 36 found to be more than normal range. Accordingto WHO criteria 15 million/ml count is said to be normal, in our research (82.0%) 557 were foundto be normal, in (2.9%) 20 count was found to be less than 15 million/ml and in (5.9%) 40 countwas less than 1 million/ml. In (9.1%) 62 counts was found to be abnormally low. In this research(66.1%) 449 had normal sperm motility, (21.8%) 148 had less than 40% and abnormally lowsperm motility was found in (12.1%) 82 males. Conclusion: The results of the single semenanalysis are of limited utility and no decision should be taken on the bases of these results interm of diagnosis and treatment strategies.

10

MWAKYOMA, HENRY A., and ERICK P. MAGOROSA. "SEMEN pH;." Professional Medical Journal 19, no. 02 (February 22, 2012): 234–41. http://dx.doi.org/10.29309/tpmj/2012.19.02.2019.

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Background: Male infertility is a frequent reproductive health problem in the world. It is usually related to abnormal spermproduction or function and these abnormalities can occur anywhere in the production of sperm including hormonal regulation, storage andtransport of sperm. Various factors are known to be responsible for seminal fluid abnormalities. Usually the first step in evaluating for maleinfertility is semen analysis. Setting: The study was conducted at Muhimbili National Hospital mainly in the department of Pathology laboratory,cytology unit. Study design: The study was a hospital based cross-sectional type. Objective: To determine semen pH in patients presenting withinfertility complains and relate how pH of seminal fluid and other semen parameters influence each other. Material and methods: Beforesemen collection, patients were instructed to abstain from sexual intercourse for a minimum of 48 hours to 7 days and collect semen bymasturbation and transport it to the laboratory through shirt pocket. Semen was to reach the laboratory for examination in not more than onehour from time of collection. Semen was examined macroscopically for volume, colour, viscosity and pH by using a pH meter (Consort C830)followed by microscopic examination which included motility of spermatozoa and sperm count by using Neuber counting chamber. The smearwas made on glass slides, fixed in 95% ethyl alcohol for 30 minutes then stained by using Papanicolaou’s staining technique and then analyzedmicroscopically for morphological examination. Results: In the analysis of the influence of semen parameters on semen pH, there wasdecrease in seminal fluid pH with age whereby as age increased the pH of seminal fluid decreased.. The general trend observed was that thepH of seminal fluid tended to decrease with an increase in the days of abstinence. pH tended to decrease with an increase of seminal fluidvolume. The pH of seminal fluid also increased with an increase in viscosity (Hyperviscosity >Hypoviscosity). The pH of seminal fluid in patientswith less than 50% forward progressive movement of spermatozoa was higher when compared to those with more than 50% forwardprogressive movement. pH had an influence on the motility of spermatozoa. Conclusions and recommendation: pH and other parameterstended to have an influence each other during seminal analysis in our study. There is a variation of pH in different parts of the World according tothe studies done. It is recommended that pH should be included during seminal analysis because our study has shown that it affects most of theseminal fluid parameters in and contribute to the problem of infertility.

Більше джерел

Дисертації з теми "Semen":

1

Badenoch, D. F. "Semen analysis and fertility." Thesis, University of Oxford, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.375208.

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2

Carson, Claire Elizabeth. "Risk factors for poor semen quality : a study of men undergoing semen analysis." Thesis, London School of Hygiene and Tropical Medicine (University of London), 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.424325.

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3

Vilakazi, David Mxolisi. "Factors affecting the quality of semen of AI dairy bulls in South Africa." Pretoria : [s.n.], 2005. http://upetd.up.ac.za/thesis/available/etd-09022005-150724.

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4

Farrás, Marcel Cavalcanti [UNESP]. "Avaliação da fragmentação do DNA espermático de sêmen refrigerado de garanhões." Universidade Estadual Paulista (UNESP), 2012. http://hdl.handle.net/11449/98144.

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Made available in DSpace on 2014-06-11T19:29:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-01-26Bitstream added on 2014-06-13T18:58:50Z : No. of bitstreams: 1 farras_mc_me_botfmvz.pdf: 544796 bytes, checksum: 513d0af00d97f12c80a9d388b7fd8f78 (MD5)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Nas últimas décadas, muitos estudos têm sido realizados nas diferentes espécies com o intuito de se determinar os fatores envolvidos no processo da fragmentação do DNA espermático. A biologia molecular voltada à área da reprodução tem resultado em inúmeras técnicas para a avaliação da qualidade da cromatina e determinação da fragmentação do DNA espermático. Em trabalhos recentes, um novo teste denominado Halomax® tem se mostrado tão eficiente quanto a outros tradicionais para a avaliação da fragmentação do DNA espermático, apresentando como vantagens a sua praticidade e o fato não requerer o uso de equipamentos de alto custo. O presente experimento teve por objetivo comparar as raças Mangalarga Marchador (MM) e Quarto de Milha (QM) quanto à resistência à refrigeração, bem como utilizar o Teste Dispersão de Cromatina Espermática denominado Halomax® para avaliar a fragmentação da cromatina espermática do sêmen refrigerado dos garanhões dessas raças em duas temperaturas de armazenamento de sêmen refrigerado (5°C e 15°C). Os resultados deste trabalho demonstraram que não houve diferença entre as temperaturas de armazenamento (5°C e 15°C), mostran do que ambas são eficientes na manutenção da viabilidade espermática pelo período de 24 horas para ambas as raças estudadas. Além disso, foi observada uma característica espermática superior dos garanhões da raça Quarto de Milha quando comparada aos da raça Mangalarga Marchador, tanto nos parâmetros de velocidade espermática, avaliado pelo CASA, quanto com relação à fragmentação do DNA espermático, revelando uma maior sensibilidade dos animais da raça Mangalarga ao processo de refrigeração do sêmen
Many studies have been conducted in different species in order to determine the factors involved in the process of sperm DNA fragmentation. Molecular biology studies focused on the reproductive area has resulted in several techniques for assessing the quality of chromatin and sperm DNA fragmentation. Recently, a new test called HALOMAX ® has been shown to be as efficient as other traditional assessment of sperm DNA fragmentation, having the advantage of practicality and not require the use of expensive equipment. The aim of this study was to compare breeds Mangalarga and Quarter Horses for cooling resistance and use the Sperm Chromatin Dispersion Test called HALOMAX ® to evaluate the sperm chromatin fragmentation using two cooled semen storage systems (5°C and 15°C). Our results showed no difference between storage systems (5°C and 15°C), showing that both are effective in maintaining sperm viability for a period of 24 hours for both breeds studied. In addition, the Quarter Horse shown superiority in semen quality parameters assessed by CASA and sperm DNA fragmentation when compared with the Mangalarga, revealing higher sensitivity of the Mangalarga stallions for cooling semen

5

Falleiros, Marcel Barbosa [UNESP]. "Avaliação comparativa do sêmen ovino refrigerado nos meios Glicina-Gema-Leite, Glicina-Gema purificada-Leite e Glicina-extrato de lipoproteínas de baxa densidade-Leite." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/98149.

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Made available in DSpace on 2014-06-11T19:29:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-01-29Bitstream added on 2014-06-13T19:17:39Z : No. of bitstreams: 1 falleiros_mb_me_botfmvz.pdf: 753532 bytes, checksum: 09df2201bbfb5625659993b6522a5251 (MD5)
Universidade Estadual Paulista (UNESP)
Objetivou-se estudar os efeitos do extrato de lipoproteínas de baixa densidade sobre o sêmen ovino durante a refrigeração. Vinte amostras de 5 carneiros foram refrigeradas por 24 e 48 horas na geladeira para refrigeração Minitube, nos meios diluentes Glicina Gema Leite, Glicina Gema purificada Leite e Glicina Extrato Leite e submetidas a teste de exaustão (37ºC/240 minutos) sendo avaliados in vitro por meio das análises da cinética espermática computadorizada, da morfologia e da integridade da membrana plasmáticas. Após 24 e 48 horas de refrigeração, os meios Glicina Gema purificada Leite e Glicina Extrato Leite apresentaram resultados superiores ao meio Glicina Gema Leite, após o teste de exaustão, para o parâmetro de integridade de membrana plasmática. Para a integridade de acrossomo o meio Glicina Gema purificada Leite foi superior (P<0,05) em relação ao meio Glicina Gema Leite durante o teste de exaustão. Nos demais parâmetros estudados de cinética espermática e morfologia (cauda dobrada), não houveram diferenças significativas (P>0,05) entre os meios. Entre os momentos, houve diferença significativa (P<0,05) em todos os meios durante o teste de exaustão
The objective was to study the effects of extract of low density lipoproteins on ovine semen during cooling. Twenty samples of five sheep were chilled for 24 and 48 hours in the refrigerator for cooling Minitube, in extenders Glycine Yolk Milk, Glycine purified Yolk Milk and Glycine Extract Milk and tested to exhaustion (37 ° C/240 min) were evaluated in vitro by means of computerized analysis of sperm kinetics, morphology and plasma membrane integrity. After 24 and 48 hours of refrigeration, the extenders Glycine purified Yolk Milk and Glycine Extract Milk showed better results than extender Glycine Yolk Milk, after the exhaustion test, for the parameter of membrane integrity. For the integrity of the acrosome through Glycine purified Yolk Milk was higher (P <0.05) than Glycine Yolk Milk during the exhaustion test. In other parameters of sperm kinetics and morphology, there were no significant differences (P> 0.05) among extenders. Between times, significant difference (P <0.05) in all extenders during the exhaustion test

6

Arman, Chairussyuhur. "Use of compounds of epididymal and plant origin in maintaining viability of ram spermatozoa." Title page, contents and summary only, 1996. http://web4.library.adelaide.edu.au/theses/09PH/09pha727.pdf.

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Bibliography: leaves 267-292. This study aimed at finding better ways of storing ram semen at refrigerator or room temperature with particular reference to ingredients readily available in Indonesia, namely coconut extract and quail egg yolk. Coconut extract showed consistent advantages with regard to sperm motility and quail egg yolk was as effective as hen egg yolk. Investigations were extended to examine storage for subnormal semen such as would be produced during periods of heat stress. Motility was assessed visually and using a Hamilton Thorn semen evaluation apparatus.

7

Dirscherl, Friederike Karin Maria [Verfasser]. "Effects of semen collection practices on the bacterial load of stallion semen / Friederike Karin Maria Dirscherl." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2011. http://d-nb.info/1013294599/34.

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8

Gacem, Sabrina. "Computerised analysis of semen in equids." Doctoral thesis, Universitat Autònoma de Barcelona, 2021. http://hdl.handle.net/10803/672020.

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L’enfocament que es proposa en aquesta tesi es basa en el fet que hi ha un buit d’informació sobre l’optimització de l’examen de la solidesa reproductiva juntament amb els protocols d’anàlisi de semen en els equids, tal com es va veure al capítol anterior. A l’ase, no es van definir protocols específics per a l’anàlisi de semen, només per a mostres de sementals on hi ha un acord general sobre el protocol a utilitzar. Quan es comprova el tracte reproductor, s’utilitza una ecografia en mode B com a mètode de diagnòstic fàcil i no invasiu. D’altra banda, es pot utilitzar una ecografia Doppler polsada per estudiar la perfusió testicular de sang. Els índexs de Doppler pulsat de l’artèria testicular i les mides ASG van mostrar una correlació positiva amb la qualitat del semen en sementals i diferents espècies. Malauradament, no es van fer estudis d’aquest tipus en ruc, cosa que constitueix el primer objectiu de la tesi. No obstant això, quan es tracta d’anàlisi de semen, la tecnologia CASA representa el millor mètode objectiu que s’utilitza avui en dia. Permet obtenir dades quantitatives fiables, fins i tot si és necessari definir protocols que assegurin la consistència i l’aplicació universal dels resultats. Tanmateix, aquesta estandardització mai s’ha fet seguint un punt de vista integrador, que compon el segon objectiu general. Hi ha tres aspectes principals a tenir en compte a l’hora d’optimitzar les anàlisis automatitzades de semen mitjançant la tecnologia CASA-Mot, a saber, el tipus, la profunditat de la cambra de comptatge, la dilució i la velocitat de fotogrames d’adquisició d’imatges. Els avenços tecnològics van permetre ara l’adquisició d’alta freqüència de captura i, al mateix temps, el procés d’aquestes imatges en segons. A més, es va definir l’estructura de les subpoblacions d’espermatozoides en marcs més alts i la distribució SP entre les races dels sementals. El tercer objectiu era col·laborar en l’ús i la millora de la nova tècnica Trumorph®, per a l’anàlisi de morfologia de l’esperma que permet l’anàlisi d’espermatozoides immobilitzats vius.
El enfoque defendido en esta tesis se basa en el hecho de que existe un vacío de información con respecto a la optimización del examen de solidez reproductiva junto con los protocolos de análisis de semen en équidos, como se vio en el capítulo anterior. En burro, no se definieron protocolos específicos para el análisis de semen, solo para muestras de sementales donde existe un acuerdo general sobre el protocolo a utilizar. Cuando se comprueba el tracto reproductivo, la ecografía en modo B se utiliza como un método de diagnóstico fácil y no invasivo. Por otro lado, la ecografía Doppler pulsado se puede utilizar para estudiar la perfusión sanguínea testicular. Los índices de Doppler pulsado de la arteria testicular y los tamaños de ASG mostraron una correlación positiva con la calidad del semen en sementales y diferentes especies. Lamentablemente, estos estudios no se realizaron en burro, lo que constituye el primer objetivo de la tesis. Sin embargo, cuando se trata de análisis de semen, la tecnología CASA representa el mejor método objetivo utilizado en la actualidad. Permite obtener datos cuantitativos confiables, incluso si es necesario para definir protocolos asegurando la consistencia y aplicación universal de los resultados. Sin embargo, esta estandarización nunca se ha hecho siguiendo un punto de vista integrador, que compone el segundo objetivo general. Hay tres aspectos principales a considerar al optimizar los análisis de semen automatizados mediante la tecnología CASA-Mot, a saber, el tipo, la profundidad de la cámara de recuento, la dilución y la velocidad de cuadros de la adquisición de imágenes. Los avances en tecnología permitieron ahora la adquisición de alta frecuencia de captura y al mismo tiempo el proceso de estas imágenes en segundos. Además, se definió la estructura de las subpoblaciones de espermatozoides en cuadros más altos y la distribución de SP entre las razas de sementales. El tercer objetivo fue colaborar en el uso y la mejora de la nueva técnica Trumorph®, para el análisis de la morfología espermática que permite el análisis de espermatozoides inmovilizados vivos.
The approach advocated in this thesis is based on the fact that there is a gap of information regarding optimization of breeding soundness examination along with semen analysis protocols in equids as seen in the previous chapter. In donkey, no specific protocols for semen analysis were defined, only for stallion samples where there is a general agreement about the protocol to use. When the reproductive tract is checked, B-mode ultrasonography is used as a non-invasive and easy diagnostic method. On the other hand, pulsed Doppler ultrasonography can be used to study testicular blood perfusion. Pulsed-Doppler indices of testicular artery and ASG sizes showed a positive correlation with semen quality in stallion and different species. Unfortunately, no such studies were done in donkey, which makes up the first objective of the thesis. However, when it’s about semen analysis, CASA technology represents the best objective method used today. It permits to obtain reliable quantitative data, even if it is needed to define protocols assuring the consistency and universal application of the results. However, this standardization has never been done following an integrative point of view, which composes the second general objective. There are three main aspects to consider when optimizing automated semen analyses by CASA-Mot technology, namely the type, depth of the counting chamber, the dilution, and the frame rate of image acquisition. The advances in technology allowed now the acquisition of high capture frequency and at the same time the process of these images in seconds. Also, was defined sperm subpopulations structure at higher frames and SP distribution between stallions’ breeds. The third objective was to collaborate in the use and the improvement of the new Trumorph® technique, for sperm morphology analysis which permits the analysis of alive immobilized sperm.
Universitat Autònoma de Barcelona. Programa de Doctorat en Medicina i Sanitat Animals

9

Mataveia, Gracinda Andre. "Effect of heterologous seminal plasma and semen extenders on motility of frozen-thawed ram sperm." Diss., Pretoria : [s.n.], 2006. http://upetd.up.ac.za/thesis/available/etd-05142008-123139/.

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10

Vogler, Cheryl Jean. "Effects of elevated testicular temperature on viability of cryopreserved semen and morphological characteristics of ejaculated spermatozoa." Thesis, Virginia Tech, 1990. http://hdl.handle.net/10919/42217.

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Анотація:

Two successive ejaculates were collected from six mature Holstein bulls at 3 d intervals for 7 wks. Elevated testicular temperature was induced by complete coverage of the scrotum with insulated material for 48 h. Viability (motility and acrosome integrity) and morphological characteristics of sperm before and after thermal insult were examined. For assessment of results, collection days were grouped: days -6, -3, 0 = Period 1 (d 0 = day of testis coverage after semen collection on that day), days 3, 6, 9 = Period 2 , days 12, 15...39 = Period 3. Semen was cryopreserved on each day of collection until morphological abnormalities of sperm increased to >50%. Semen viability before and after freezing was lower in Period 3 than in Period 1 (P≤.01). These differences coincided with increased abnormal morphology. No differences in viability were observed between Period 1 and Period 2 for unfrozen semen. Once frozen, spermatozoa ejaculated during Period 2 were significantly different from Period 1 for both viability measurements, but only after 3 h incubation at 37°C (P≤.01). Mean percent pre-insult abnormal sperm level was 19.6 ± 5.7 and sperm morphology in Period 1 (pre-insult) did not differ from that in Period 2. Morphological change was first noted in Period 3 on d 12 and 15 (47.5 ± 27.4 and 65.0 ± 27.0 % abnormal sperm, respectively). Abnormal sperm peaked on d 21 (83.2 ± 22.8 %). Although bulls varied in degree and time of response post-insult, all bulls exhibited the same sequence of appearance for specific abnormalities. The sequence and peak means for these abnormalities observed over all bulls were as follows: decapitated sperm, d 15 (33.9 ± 28.8 %); diadem defect, d 18 (55.6 ± 25.8 %); pyriform heads and nuclear vacuoles (excluding diadems), d 21 (18.3 ± 17.6 and 20.8 ± 10.5 %, respectively); knobbed acrosomes, d 27 (11.6 ± 13.6 % ). Sperm morphology was followed through d 39, by which time all bulls were producing ≤50% abnormal cells (35.2 ± 8.0 %). We concluded that viability of epididymal/rete sperm was adversely affected by elevated testicular temperatures, as noted by lowered viability of cryopreserved semen, and that there is a sequence in appearance of abnormal cell types in repsponse to thermal insult of the testis.
Master of Science

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Книги з теми "Semen":

1

García, Yunior. Semen. Ciudad de La Habana, Cuba: Casa Editora Abril, 2012.

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2

Tubau, Iván. Semen. Alzira, Valencia: Edicions Bromera, 2003.

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3

Vitoshnev, S. Semen Budennyĭ. Minsk: OOO "Kuzʹma", 1998.

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4

Hrabovet͡sʹkyĭ, V. V. Semen Vysochan. Ivano-Frankivsʹk: Kafedra istoriï Ukraïny Prykarpatsʹkoho universytetu im. V. Stefanyka, 1995.

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5

Demin, Lev Mikhaĭlovich. Semen Dezhnev. Moskva: "Molodai︠a︡ Gvardii︠a︡", 1990.

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6

Alʹtov, S. Semen Alʹtov. Moskva: Ėksmo, 2004.

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7

Galen. On semen. Berlin: Akademie Verlag, 1992.

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8

Pantouw, Ellen R. Sukses usaha bersama Semen Indonesia: Success with Semen Indonesia. Jakarta: Biro Program Kemitraan, PT. Semen Indonesia (Persero), Tbk., 2013.

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9

Marrodán, Mario Angel. Semen de santo. Vigo, España: Ediciones Cardeñoso, 1989.

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10

I︠A︡kubovich, Semen. Semen Iakubovich predstavliaet--. Kharʹkov: Madrid, 2012.

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Частини книг з теми "Semen":

1

Gooch, Jan W. "Semen." In Encyclopedic Dictionary of Polymers, 923. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_14771.

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2

Glover, T. D. "Semen Analysis." In Advances in Clinical Andrology, 15–29. Dordrecht: Springer Netherlands, 1988. http://dx.doi.org/10.1007/978-94-009-1237-3_3.

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3

Burch, Rebecca L. "Semen Familiarity." In Encyclopedia of Evolutionary Psychological Science, 1–2. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-16999-6_2012-1.

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4

Wolfner, Mariana F., and Tracey Chapman. "Semen Toxicity." In Encyclopedia of Evolutionary Psychological Science, 1–5. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-16999-6_3081-1.

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5

Taymor, Melvin L. "Semen Analysis." In Infertility, 89–98. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4613-0627-6_12.

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6

Cooper, Trevor G. "Semen Analysis." In Andrology, 125–38. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-78355-8_9.

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7

Ridley, John W. "Semen Evaluation." In Fundamentals of the Study of Urine and Body Fluids, 277–300. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-78417-5_12.

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8

McCue, Patrick M. "Semen Freezing." In Equine Reproductive Procedures, 436–40. Hoboken, NJ, USA: John Wiley & Sons, Inc, 2014. http://dx.doi.org/10.1002/9781118904398.ch133.

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9

Ax, R. L., M. Dally, B. A. Didion, R. W. Lenz, C. C. Love, D. D. Varner, B. Hafez, and M. E. Bellin. "Semen Evaluation." In Reproduction in Farm Animals, 363–75. Baltimore, Maryland, USA: Lippincott Williams & Wilkins, 2016. http://dx.doi.org/10.1002/9781119265306.ch25.

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10

Zambelli, Daniele, and Marco Cunto. "Semen Cryopreservation." In Feline Reproduction, 213–22. GB: CABI, 2022. http://dx.doi.org/10.1079/9781789247107.0023.

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Тези доповідей конференцій з теми "Semen":

1

Jiang, Yufeng, Shiying Zheng, Chunyin Yan, and Wei Zhang. "The application of computer-assisted semen analysis parameters in semen quality analysis." In 2009 International Conference on Future BioMedical Information Engineering (FBIE). IEEE, 2009. http://dx.doi.org/10.1109/fbie.2009.5405901.

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2

Hong-yu, Li, Zhang Li-hua, Yuan Guang-xin, Zhou Yan-ju, Zhang Xue-li, Kong De-xin, Song Jia-yin, and Li Wei. "Research for ultramicrofingerprint of Semen Cuscutae." In 2011 International Conference on Human Health and Biomedical Engineering (HHBE). IEEE, 2011. http://dx.doi.org/10.1109/hhbe.2011.6027895.

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3

TKACHEV, Alexander V., Olga L. TKACHEVA, Lyudmila V. GAZZAVI-ROGOZINA, Tatyana V. ZUBOVA, Oksana V. SMOLOVSKAYA, and Vladimir A. PLESHKOV. "Modern Technology of Poultry Semen Cryopreservation." In IV International Scientific and Practical Conference "Modern S&T Equipments and Problems in Agriculture". Sibac, 2020. http://dx.doi.org/10.32743/kuz.mepa.2020.235-244.

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4

Zheng, Guang, Anjie Qiao, and Cheng Lv. "Therapeutic Mechanisms of Persicae Semen towards Healing Processes." In 2019 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2019. http://dx.doi.org/10.1109/bibm47256.2019.9044450.

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5

Chaudhari, Nandini M., and Bhausaheb V. Pawar. "Light scattering study on semen analysis methods/techniques." In 2013 Nirma University International Conference on Engineering (NUiCONE). IEEE, 2013. http://dx.doi.org/10.1109/nuicone.2013.6780079.

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6

Chavan, Amit R., A. R. Shastri, R. K. Shastri, and S. B. Deosarkar. "Counting of Frozen Semen Straws Using Image Processing." In 2013 Third International Conference on Advances in Computing and Communications (ICACC). IEEE, 2013. http://dx.doi.org/10.1109/icacc.2013.44.

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7

Morrell, J. M., and Margareta Wallgren. "Control of bacterial contamination in boar semen doses." In Proceedings of the International Conference on Antimicrobial Research (ICAR2010). WORLD SCIENTIFIC, 2011. http://dx.doi.org/10.1142/9789814354868_0059.

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8

Nautiyal, Krishna, and Sahil Sharma. "Forensic examination of semen and seminal stains: A review." In ADVANCEMENTS IN CIVIL ENGINEERING: COSMEC-2021. AIP Publishing, 2023. http://dx.doi.org/10.1063/5.0130314.

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9

Astuti, Endang, Sahroni Sahroni, and Anindya Wardani. "A Comparative Analysis of Financial Distress at PT. Semen Baturaja Tbk. and PT. Semen Indonesia Tbk. from the Period Year of 2012 - 2018." In Proceedings of the 1st International Conference on Economics Engineering and Social Science, InCEESS 2020, 17-18 July, Bekasi, Indonesia. EAI, 2021. http://dx.doi.org/10.4108/eai.17-7-2020.2303090.

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10

Saikat Kumar Jana, Baidyanath Chakravarty, and Koel Chaudhury. "Does oral antioxidant administration improve semen parameters in infertile men?" In 2010 International Conference on Systems in Medicine and Biology (ICSMB). IEEE, 2010. http://dx.doi.org/10.1109/icsmb.2010.5735412.

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Звіти організацій з теми "Semen":

1

Dzhoglov, Spas, Doychin Boyadzhiev, and Evgeniya Neshova Ivanova. Association between Some Environment and Lifestyle Factors with Male Semen Quality Parameters: Semen Volume, Spermatozoa Concentra tion and Motility. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, January 2021. http://dx.doi.org/10.7546/crabs.2021.01.15.

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2

Weller, Joel, Harris Lewin, Micha Ron, and George Wiggans. Detection and Mapping of Genes Affecting Traits of Economic Importance in Dairy Cattle with the Aid of Molecular Genetic Markers. United States Department of Agriculture, December 1995. http://dx.doi.org/10.32747/1995.7613024.bard.

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Forty-seven poly-TG microsatellites were developed at the U of IL, and 11 genetic markers were developed at ARO, nine of which were poly-AGC microsatellites. Markers were typed on the reference families of CSIRO, Australia; GRANADA, Texas; and IRRF, Illinois, for chromosome assignment and linkage mapping. Nine North American al organizations contributed semen to the Dairy Bull DNA Repository (DBDR), which currently has 65,743 units from 3366 bulls. Semen was obtained for 31 out of 35 grandsires. Semen of 28 and 23 sons of two Israeli bulls was also collected. Eighteen grandsires were genotyped for 75 microsatellites. One thousand, three hundred and sixty-two sons with evaluation from 17 families were genotyped for 24 markers. Eleven thousand, six hundred and twenty sons genotypes were determined, of which 8,802 were informative. The genotype data was matched to the bulls' daughter yield deviations (DYD) for seven traits; milk, fat, and protein production; fat and protein percent; somatic cell concentration (SCS); and productive herd life. Seven loci had significant effects at p<0.05, but only two loci, TGLA263 and MGTG7, had significant effects at p<0.01, and the effect of TGLA263 on fat percentage was significant at p<0.0001. There was at least one significant effect for each of the seven traits analyzed.

3

Mabry, John. Comparison of Semen Delivery Systems for Commercial Swine Production in Iowa. Ames (Iowa): Iowa State University, January 2004. http://dx.doi.org/10.31274/ans_air-180814-97.

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4

Ouzounova-Raykova, Vessela, Radoslav Baykushev, Mohamed El Tibi, Simeon Rangelov, Dragomir Petrov, and Ivan Mitov. Genital Mycoplasmas and Ureaplasmas and Abnormal Semen Quality in Infertile Bulgarian Men. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, July 2018. http://dx.doi.org/10.7546/crabs.2018.07.15.

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5

Phillips, Patrick E., Jacqueline N. Myers, and Grant A. Dewell. Viability of Tritrichomonas foetus Following the Freeze-Thaw Cycle Used for Freezing Bovine Semen. Ames (Iowa): Iowa State University, January 2016. http://dx.doi.org/10.31274/ans_air-180814-571.

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6

Weller, Joel, Harris Lewin, Micha Ron, George Wiggans, and Paul VanRaden. A Systematic Genome Search for Genes Affecting Economic Traits Dairy Cattle with the Aid of Genetic Markers. United States Department of Agriculture, April 1999. http://dx.doi.org/10.32747/1999.7695836.bard.

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The objectives were to continue collection of semen for the US dairy bull DNA repository, to conduct a systematic search of the Holstein genome for economically significant economic trait loci (ETL), to develop and refine statistical techniques for the analysis of the data generated, and to confirm significant effects by genotyping daughters i Israel and additional US sons. One-thousand-seventy-six sons of eight US grandsires were genotyped for 174 microsatellites located on all 29 autosomes. ETL were detected for milk production traits on seven chromosomes. ETL for milk and fat yield and fat and protein percentage on BTA3 was mapped to between the markers BL41 and TGLA263. The 95% confidence interval for the ETL affecting fat percentage on BTA14 localized this ETL between the contromere and chromosome position 11 cM. This ETL was verified in the Israeli cattle population by genotyping an independent sample of cows from seven families. The radiation hybrid data for the centromeric region of BTA14 is defined by a single linkage group. Order of Type I genes within this region, CYC-FADK-TG-SQLE, is conserved between human and cattle. Thus, HSA8, the human homologue of BTA14, can be used to identify candidate genes for the ETL.

7

Adamczewska, Daria, Jolanta Słowikowska-Hilczer, and Renata Walczak-Jędrzejowska. The Association Between Vitamin D and the Components of Male Fertility: a Systematic Review. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, November 2022. http://dx.doi.org/10.37766/inplasy2022.11.0151.

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Review question / Objective: The purpose of this systematic review is to provide an up-to-date, comprehensive review of the existing literature on the experimental and clinical evidence for the effects of VD on the components of male fertility, sperm parameters and sex hormone production. Condition being studied: Vitamin D serum level in relation to men semen quality and sex hormones serum concentration. Eligibility criteria: Exclusion criteria: not in English; review, meta analysis; animal studies; in vitro studies; study group < 30 subject; insufficient data; wrong or missing outcome.

8

Hansen, Peter J., and Zvi Roth. Use of Oocyte and Embryo Survival Factors to Enhance Fertility of Heat-stressed Dairy Cattle. United States Department of Agriculture, August 2011. http://dx.doi.org/10.32747/2011.7697105.bard.

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The overall goal was to identify survival factors that can improve pregnancy success following insemination or embryo transfer in lactating dairy cows exposed to heat stress. First, we demonstrated that oocytes are actually damaged by elevated temperature in the summer. Then we tested two thermoprotective molecules for their effect on oocyte damage caused by heat shock. One molecule, ceramide was not thermoprptective. Another, insulin-like growth factor-1 (IGF) reduced the effects of heat shock on oocyte apoptosis and oocyte cleavage when added during maturation. We also used lactating cows exposed to heat stress to determine whether bovine somatotropin (bST), which increases IGF1 levels in vivo, would improve fertility in summer. Cows treated with bST received a single injection at 3 days before insemination. Controls received no additional treatment. Treatment with bST did not significantly increase the proportion of inseminated cows diagnosed pregnant although it was numerically greater for the bST group (24.2% vs 17.8%, 124–132 cows per group). There was a tendency (p =0.10) for a smaller percent of control cows to have high plasma progesterone concentrations (≥ 1 ng/ml) at Day 7 after insemination than for bST-treated cows (72.6 vs 81.1%). When only cows that were successfully synchronized were considered, the magnitude of the absolute difference in the percentage of inseminated cows that were diagnosed pregnant between bST and control cows was reduced (24.8 vs 22.4% pregnant for bST and control). Results failed to indicate a beneficial effect of bST treatment on fertility of lactating dairy cows. In another experiment, we found a tendency for addition of IGF1 to embryo culture medium to improve embryonic survival after embryo transfer when the experiment was done during heat stress but not when the experiment was done in the absence of heat stress. Another molecule tested, granulocyte-macrophage colony-stimulating factor (GM-CSF; also called colony-stimulating factor-2), improved embryonic survival in the absence of heat stress. We also examined whether heat shock affects the sperm cell. There was no effect of heat shock on sperm apoptosis (programmed cell death) or on sperm fertilizing ability. Therefore, effects of heat shock on sperm function after ejaculation if minimal. However, there were seasonal changes in sperm characteristics that indicates that some of the decrease in dairy cow fertility during the summer in Israel is due to using semen of inferior quality. Semen was collected from five representative bulls throughout the summer (August and September) and winter (December and January). There were seasonal differences in ion concentration in seminal plasma and in the mRNA for various ion channels known to be involved in acrosome reactions. Furthermore, the proportion of sperm cells with damaged acrosomes was higher in post-thaw semen collected in the summer than in its counterpart collected in winter (54.2 ± 3.5% vs. 51.4 ± 1.9%, respectively; P < 0.08Further examination is required to determine whether such alterations are involved in the low summer fertility of dairy cows.

9

Johnson, Anne. Seven Pairs. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.1591.

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10

Peterson, Donovan. Seven dyads. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.5350.

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