Готові списки джерел за темами / Purification

Добірка наукової літератури з теми "Purification"

Автор: Grafiati
Опубліковано: 4 червня 2021
Оновлено: 22 червня 2024

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Статті в журналах з теми "Purification":

1

Arakawa, Shizuka, Kohsuke Kamizaki, Yusuke Kuwana, Naruki Kataoka, Chieko Naoe, Chie Takemoto, Takashi Yokogawa, and Hiroyuki Hori. "Application of solid-phase DNA probe method with cleavage by deoxyribozyme for analysis of long non-coding RNAs." Journal of Biochemistry 168, no. 3 (May 3, 2020): 273–83. http://dx.doi.org/10.1093/jb/mvaa048.

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Abstract The solid-phase DNA probe method is a well-established technique for tRNA purification. We have applied this method for purification and analysis of other non-coding RNAs. Three columns for purification of tRNAPhe, transfer-messenger RNA (tmRNA) and 16S rRNA from Thermus thermophilus were connected in tandem and purifications were performed. From each column, tRNAPhe, tmRNA and 16S rRNA could be purified in a single step. This is the first report of purification of native tmRNA from T. thermophilus and the purification demonstrates that the solid-phase DNA probe method is applicable to non-coding RNA, which is present in lower amounts than tRNA. Furthermore, if a long non-coding RNA is cleaved site-specifically and the fragment can be purified by the solid-phase DNA probe method, modified nucleosides in the long non-coding RNA can be analysed. Therefore, we designed a deoxyribozyme (DNAzyme) to perform site-specific cleavage of 16S rRNA, examined optimum conditions and purified the resulting RNA fragment. Sequencing of complimentary DNA and mass spectrometric analysis revealed that the purified RNA corresponded to the targeted fragment of 16S rRNA. Thus, the combination of DNAzyme cleavage and purification using solid-phase DNA probe methodology can be a useful technique for analysis of modified nucleosides in long non-coding RNAs.
2

Urbański, Stanisław. "Natura drogi oświecenia według o. Pawła Ogórka OCD." Łódzkie Studia Teologiczne 23, no. 2 (June 28, 2023): 147–58. http://dx.doi.org/10.52097/lst.2023.2.147-158.

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The path of enlightenment consists imitating of Jesus by practicing the virtues, especially the supernatural ones. The condition for this development of virtues is a some purity of heart. Man has to put his passions in order and fight with his major weaknesses. Then comes self-control, order in everyday life, deep conviction of the truths of faith, and more time to talk with the Lord during prayer. To achieve this state, a Christian must to pass a process of passive purification, which leads him into the mystical life. All imperfections must be removed. Therefore, God’s intervention is needed, which is expressed through special inspirations. Then the gifts of the Holy Spirit enable us to receive them. The Christian experiences passive purifications of senses and of spirit. This state can be compared to the purification of the senses. The purification of the soul is compared to the purification of gold in a crucible. In this state, prayer develops from meditation to emotional prayer. The Christian must therefore remove the obstacles in the development of prayer.
3

Gu, Hong, Yong Zhi Zou, Zheng Bin Xu, and Jian Min Zeng. "Investigation on Purification and Degassing of TiB2/Al Composite Fabricated by LSM Method." Key Engineering Materials 353-358 (September 2007): 3051–54. http://dx.doi.org/10.4028/www.scientific.net/kem.353-358.3051.

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In the present paper, TiB2/Al composite with 5% volume fraction of TiB2 was fabricated by LSM method. The effects of purification and degassing methods on TiB2/Al composite were examined by means of X-ray diffraction (XRD) and image analysis. Hydrogen contents in the molten composites were detected and compared among flux, inert gas and vacuum purification processes. The experimental results indicate that under general cast condition a majority of the TiB2 particles distribute on grain boundary, and only a few particles disperse within grains. The flux and vacuum purifications have no virtual impact on the distribution of TiB2 and the fraction of TiB2 remains the same after purification. However, degassing with inert gas will be detrimental; the TiB2 particles will be separated and removed from the matrix. The hydrogen contents for flux, inert gas and vacuum processes are 0.15ml/100g/Al, 0.12ml/100g/Al and 0.12ml/100g/Al respectively.
4

Nelidkin, A. V., S. N. Borychev, and D. O. Oleynik. "DEVICE FOR PURIFICATION OF DIESEL ENGINE EXHAUST GASES." Vestnik Altajskogo gosudarstvennogo agrarnogo universiteta, no. 8 (2021): 92–97. http://dx.doi.org/10.53083/1996-4277-2021-202-08-92-97.

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To ensure high quality and productive work of employ-ees, it is necessary to achieve the target parameters of the microclimate and to exclude harmful and toxic substances in the atmosphere of the working area of agricultural prem-ises of a closed volume and air exchange. The main rea-son for the distortion of the air-gas regime of the room is the use of agricultural machines in closed industrial prem-ises (warehouses, storage facilities, livestock facilities, etc.). As a result, there is a decrease in the quality of prod-ucts and working conditions at agricultural enterprises, as well as a reduction in the operational life of structures. To-day, in agricultural machinery, diesel engines are most often used as power units which, unlike gasoline engines are more economical, and also reduce the harmful impact on the environment. But, despite this, the operation of die-sel engines still causes the accumulation of harmful com-ponents in the atmosphere of the room which negatively affects the health of the staff. The analysis of the designs of devices for exhaust gas purificationof internal combus-tion engines revealed the problems that affect the efficien-cy of the purification. The most significant problems are as following: large weight and dimensions, reduced efficiency of the neutralizers when the engine is running at modes close to the nominal ones, and large gas-dynamic re-sistance. To solve these problems, the design of a device for exhaust gas purificationin diesel engines was devel-oped. The use of this utility model will increase the efficien-cy of the device for exhaust gas purification. It will improve the environmental performance of the diesel engine reduc-ing emissions of harmful substances and soot into the at-mosphere.
5

Thomas, Aleena, Baptiste Gasch, Enzo Olivieri, and Adrien Quintard. "Trichloroacetic acid fueled practical amine purifications." Beilstein Journal of Organic Chemistry 18 (February 24, 2022): 225–31. http://dx.doi.org/10.3762/bjoc.18.26.

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Amine purification have for long been dominated by tedious stepwise processes involving the generation of large amounts of undesired waste. Inspired by recent work on out of equilibrium molecular machinery, using trichloroacetic acid (TCA), we disclose a purification technique considerably decreasing the number of operations and the waste generation required for such purifications. At first, TCA triggers the precipitation of the amines through their protonated salt formation, enabling the separation with the impurities. From these amine salts, simple decarboxylation of TCA liberates volatile CO2 and chloroform affording directly the pure amines. Through this approach, a broad range of diversely substituted amines could be isolated with success.
6

Andersen, Astrid Oberborbeck. "Purification." Science, Technology, & Human Values 43, no. 3 (August 2, 2017): 379–400. http://dx.doi.org/10.1177/0162243917723079.

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In Arequipa, Peru’s second largest city, engineers work hard to control water flows and provide different sectors with clean and sufficient water. In 2011, only 10 percent of the totality of water used daily by Arequipa’s then close to 1 million people—in households, tourism, industry, and mining—was treated before it was returned to the river where it continues its flow downstream towards cultivated fields and, finally, into the Pacific Ocean. It takes specialized knowledge and manifold technologies to manage water and sustain life in Arequipa, and engineers are central actors for making water flow. Examining the ecology of water management, this article asks to what extent we can talk of a way of knowing and enacting water that is particular to engineers. Through engineering practices, a technical domain emerges as separate from and superior to political and social domains. This production of categories can be understood as practices of purification. However, a purely technical grip on water is never possible. Unruly elements, like weather, contamination, urban dwellers, and competing interests, interfere and make processes of intervention unstable. Water is never completely cleaned, and, equally, the continuous processes of purification of categories and domains take place while other processes work to blur their boundaries.
7

Khoirudin, Azaki, and Qaem Aulassyahied. "Reinterpreting Muhammadiyah’s Purification and Its Implications for the Educational Philosophy." Progresiva : Jurnal Pemikiran dan Pendidikan Islam 12, no. 02 (December 7, 2023): 161–78. http://dx.doi.org/10.22219/progresiva.v12i02.29596.

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This article mainly explores the changing and development of thought that occurred in one of the largest modernist Islamic organizations in Indonesia, Muhammadiyah, regarding Islamic purification. Using intellectual history as an approach, this research concluded that even though Muhammadiyah had purification as its main characteristic, in fact it had its own reinterpretation being extended and expanded. By implementing Abdolkarim Soroush’s theory of contraction and expansion of religious knowledge, this research found that in the very early days of its inception, Muhammadiyah constructed its concept of purification from the spiritualization, rationalization, and functionalization of Islamic teachings. But after the inception of Tarjih division in 1927, the purification in Muhammadiyah had a narrower orientation from worships and creeds (puritanism) to the muamalah of world affairs (ultra-puritanism). Beginning in 1995, there was a wider scope in which the Tarjih division in Muhammadiyah expanded its issues of worship to include public and humanitarian issues; that era was called post-puritanism. Based on the core hypothesis that any changes in religious thought have implications for educational orientation, from George R. Knight’s perspective, several educational implications are obtained: (1) In the early days of its inception, Muhammadiyah’s education implemented a progressivism philosophy that’s inspired by religion, rationality, and life; (2) when it comes to the narrower aspect of purification only for faith, worship, and muamalah, Muhammadiyah shifted its educational philosophy to perennialism-essentialism; (3) when the purification's meaning and interpretation expanded, Muhammadiyah tended its education toward the philosophy of reconstructionism.
8

Guo, Zhimou, Yuansheng Xiao, Yanfang Liu, Tu Liang, and Xinmiao Liang. "Purification of chitooligosaccharides by using purification factory." Journal of Biotechnology 136 (October 2008): S83. http://dx.doi.org/10.1016/j.jbiotec.2008.07.187.

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9

Malyovannyi, Myroslav, Galina Sakalova, Natalia Chornomaz, and Oleh Nahurskyy. "Water Sorption Purification from Ammonium Pollution." Chemistry & Chemical Technology 7, no. 3 (September 25, 2013): 355–58. http://dx.doi.org/10.23939/chcht07.03.355.

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10

Odiegwu C.N.C., Ukaejiofo E.O., Tothill I.E., Chianella I., and Okey-Onyesolu C.F. "Purification and Characterisation of Lectin Isolated from Nigeria Achatina achatina Snail." GSC Advanced Research and Reviews 5, no. 3 (December 30, 2020): 001–9. http://dx.doi.org/10.30574/gscarr.2020.5.3.0095.

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Lectins are carbohydrate-binding proteins that are highly specific for sugar moieties of other molecules. They perform recognition on the cellular and molecular level and play numerous roles in biological recognition phenomena involving cells, carbohydrates, and proteins. Blood groups are inherited characters which give rise to antigen-antibody reaction. A total of 120 samples of local (Nigeria) Achatina achatina snail specie were collected, authenticated at the Zoology Department of the University of Nigeria, Nsukka and 80mls of pooled crude lectin extract was obtained. Purifications were performed on 20mls of the crude extract in three steps viz, Ammonium sulphate precipitation and Dialysis (Partial purifications), Con A Sepharose 4B affinity chromatography column (Complete purification). The affinity purified lectin was used for all the actual tests conducted in this research. The crude, partially and complete/affinity purified lectin extracts were subjected to Haemagglutination tests, Protein Assay and Specific Sugar determinations. The molecular weight was assessed by Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) method. The results of the research showed as follows: On complete/affinity purification, 15mls of pure sample containing only the high molecular weight lectin was obtained. The respective haemagglutination tests on the crude, partially and affinity purified lectin showed on standardisation, preferential agglutination with Blood group A type. The Protein contents of the lectin was deduced to be as follows: The crude extract contains 13.5mg/dl, Dialysed precipitate – 5.7mg/dl, Dialysed supernatant – 5.0mg/dl and the Affinity purified Lectin – 0.422mg/dl. Galactose N-acetyl amine (Gal NAc) residue was determined to be its specific sugar. The SDS-PAGE analysis showed the molecular weight of the lectin to be 250 KDaltons. This research has therefore succeeded in the Purification, Characterisation and illustration of the lectinic properties of the local Nigeria snail - Achatina achatina.
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Статті в журналах Дисертації Книги

Дисертації з теми "Purification":

1

Du, Plessis Jan-Paul. "The Purification Works." Diss., University of Pretoria, 2018. http://hdl.handle.net/2263/63619.

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This dissertation aims to propose an appropriate architectural intervention within a site that requires both ecological restitution and the commemoration of industrial heritage. The Johannesburg Gasworks site serves as a clear example of how the Industrial Revolution and subsequent industrial technologies have both damaged the natural environment and left blighted legacies within ever developing urban conditions.The project aims to uphold the general significance of Industrial heritage as proposed by charters such as the Nizhny Tagil charter prepared by The International Council for the Commemoration of Industrial Heritage as well as the unique heritage significance of the Gasworks site. An appropriate theoretical framework and precedents are explored that reconcile the two seemingly opposing requirements of post-industrial sites - that of commemoration and ecological restitution. In post-industrial sites scarred by water, soil and air pollution, as well as dangerous or inaccessible places, maintaining an appreciation of heritage whilst employing the various rehabilitative actions required need to be balanced to ensure both. The project undertaken forms part of four schemes proposed for the site that aim to maintain the iconic identity of the Johannesburg Gasworks by proposing ecologically sensitive industries. These industries and interventions within the site aim to bring about urban resilience, site specific environmental rehabilitation as well as integration with the surrounding urban context. The proposed project for the site draws its program from global ecological issues as well as site specific heritage factors. The aim of scripting a new layer of intervention onto the Gas Works site is to make a legible reading between the site’s history and its ecologically resilient future legacy.
Mini Dissertation MArch(Prof)--University of Pretoria, 2018.
Architecture
MArch(Prof)
Unrestricted
2

Hiltbrunner, Jean-Michel. "Purification de l'hexafluorure d'uranium." Thesis, Université Clermont Auvergne‎ (2017-2020), 2017. http://www.theses.fr/2017CLFAC009.

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L’hexafluorure d’uranium, de formule chimique UF 6 , est un composé clé du cycle du combustible car il est utilisé pour l’étape d’enrichissement isotopique. Toutefois, des impuretés présentes et mélangées à l’UF 6 sont à éliminer afin de garantir un uranium de pureté nucléaire. Dans ce manuscrit, les réactions chimiques entre les polluants et les filtres chimiques retenus pour la purification sont étudiées. Par ailleurs, l’étude de la réactivité en milieu liquide est réalisée afin de se rapprocher des conditions industrielles. Une voie de recyclage des filtres chimiques est également investiguée afin de réutiliser les adsorbants sur plusieurs cycles de purification. L’ensemble des performances (taux de sorption des polluants, décontamination lors du recyclage) sont évaluées avant la mise au point du pilote à l’échelle industrielle
Uranium hexafluoride, which chemical formula is UF 6 , is a key compound of nuclear fuel cycle due to its use during isotopic enrichment process. Nethertheless, pollutants melted with UF 6 have to be removed in order to ensure a nuclear purity fuel. In this manuscript, the reactions occurring between pollutants and chemical filters selected for this application are studied. Then, the reactivity in liquid UF 6 is also examined with the aim of being close to the industrial process. The regeneration of adsorbents is investigated so that chemical filter can be used for several purification cycles. The performances (sorption rate of pollutants, purifying during recycling step) are evaluated before simulations at industrial scale
3

Allaire, André. "Copper matte vacuum purification." Thesis, McGill University, 1991. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=70215.

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An investigation of copper matte vacuum purification was undertaken. A mathematical model using monatomic, diatomic sulphide and diatomic oxide vapours of the impurities was developed to explain the vacuum refining process. The model was used to predict the overall refining rates for variables such as temperature, matte grade, oxygen activity and chamber pressure of the melt.
A series of experiments was undertaken to characterize the dust produced during vacuum refining of copper matte. An attempt to selectively condense the vapours produced during the vacuum refining experiments was carried out.
The "Lift-Spray" vacuum refining process was used to refine 15 to 40 kg batches of molten copper matte containing 35 to 78% copper. The removal rates of lead, bismuth, arsenic, antimony, selenium, nickel and silver were measured under different levels of matte grade, chamber pressure, lifting gas flow rate and dissolved oxygen content in the melt. The ranges of the variables under study were from 10 to 600 pascals chamber pressure, 0 to 40 normal milliliter per second of lifting gas flow rate and 10$ sp{-16}$ to 10$ sp{-7}$ atmosphere of oxygen activity.
In conclusion, LSV refining of copper matte was shown to be a promising process. Furthermore, scale-up to industrial size is now possible. The scale-up dimensions compare well to the dimensions of the RH degassing unit presently used in the steel industry. (Abstract shortened by UMI.)
4

Kent, Laura. "Photocatalysts for water purification." Thesis, University of Surrey, 2018. http://epubs.surrey.ac.uk/850035/.

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Advanced water purification methods are required to answer the growing demand for clean water throughout the world. Current methods of removing the pollutants rely on moving the pollutants from one place to another rather than breaking them down. The use of advanced oxidative processes (AOPs) presents a highly effective opportunity to achieve the full mineralisation of pollutants without the added cost of regeneration methods. Photocatalysts, such as titanium dioxide and zinc oxide, can be used as AOPs when activated by electromagnetic radiation in the form of ultraviolet and visible light. To facilitate the activation with visible light, titanium dioxide doped with rare earth elements was produced via a sol gel method. Both single doped and co-doped systems were investigated with efficiency determined by the percentage of degraded methylene blue over 48 hours under ultraviolet filtered visible light. The incorporation of rare earth ions restricted the growth of the more active anatase phase and the method produced highly agglomerated, sintered nano particles which exhibited as micron sized particles. The highest methylene blue removal rate achieved in 48 hours for a single doped system was 70% for the 1 mol% yttrium doped titanium dioxide. This was improved further on inclusion of 1 mol% praseodymium which showed an 86% removal of methylene blue over the same time period. The coating of known up-converting phosphors with the successfully developed doped titanium dioxide was investigated. Yttrium silicate doped with praseodymium and lithium, was found to be the most successful known phosphor when used with the commercially available P25 titanium dioxide. When coated with the doped titanium dioxide shell at a 2:1 ratio of phosphor to titanium dioxide, a methylene blue degradation of 94% was reached. Initial tests on the coating of titanium dioxide with the known up-converting phosphor showed that methylene blue was absorbed rather than broken down so was not developed further. An investigation into the incorporation of zinc oxide, both pure and doped with the same successful titanium dioxide system was carried out. Zinc oxide shells were coated onto doped titanium dioxide, the known up-converting phosphor and the doped titanium dioxide coated known phosphor. The crystalline form of zinc oxide was inhibited by the incorporation of rare earth ions, as with the titanium dioxide system, and from the thickness of the zinc oxide shell. The highest degradation achieved was a 91% removal rate for the ZnO-PrY:TiO2-PrY:Y2SiO5-Pr,Li core shell shell structure indicating there was no further improvement on incorporation of zinc oxide, either doped or un-doped.
5

Rutt, George Clifford. "Purification of recombinant proteins." Thesis, Massachusetts Institute of Technology, 1996. http://hdl.handle.net/1721.1/42614.

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6

Benzouaa, Rachid. "Purification de l'hexafluorure d'uranium." Thesis, Clermont-Ferrand 2, 2014. http://www.theses.fr/2014CLF22433.

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L’hexafluorure d’uranium (UF6), est le seul composé utilisé à l’état gazeux dans les procédés d’enrichissement pour la production du combustible nucléaire. Pour le bon déroulement de l’étape d’enrichissement, la qualité de UF6 est primordiale. Cette étude s’est intéressée principalement aux impuretés volatiles sous forme de fluorures et oxyfluorures et leur réactivité avec des matériaux en présence ou non de l’UF6. La nature des produits adsorbés et de réactions a été identifiée. Les mécanismes physico-chimiques mis en jeux lors de la sorption ont été investigués. Les performances (capacité de sorption, sélectivité et régénération) de ces matériaux absorbants et adsorbants ont été évaluées en vue de leur utilisation comme filtre UF6 dans les usines de conversion
Uranium hexafluoride (UF6), is the only compound used in the gaseous state in the process of enrichment to produce nuclear fuel. For the success of the enrichment step, the UF6 quality is paramount. This study is mainly concerned with volatile impurities in form of fluorides and oxyfluorides and their reactivity with the materials in presence or not of UF6. The nature of the adsorbed compounds and reactions products was identified. The physico-chemical mechanisms of sorption were investigated. The performances (sorption capacity, selectivity and regeneration) of the absorbent and adsorbent materials have been evaluated to be used as UF6 filters in conversion plants
7

Albornoz, Rodrigo Nicolas. "Purification : Research & Exhibition." Thesis, Konstfack, Institutionen för Konst (K), 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:konstfack:diva-6738.

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In recent years I have been working on topics related to Post-colonialism in South America, as well as the conditions of immigrants in Europe. The main interest of my work involves questions about my roots and personal stories of migration. This situation has been the engine of my art for the last ten years. Through my installations, I have tried to express or represent some certain facts, that have marked the post-colonial situation in Latin America and Europe over the last years.  The slavery and exploitation of illegal labor are common working conditions for many people in South America, especially for those located in the jungles and slums. The Indigenous culture -its languages, values and traditions- have begun to disappear and been displaced by Western culture. Here in Europe, on the other hand, I feel 'alien' to the territory of my ancestors, due to the fact that my family comes from European immigrants in Argentina. Illegal labor has been also a part of my life, having experienced the condition of 'otherness' in Europe. Therefore, this project reflects upon my personal experience in relation to the unfair conditions of labor in autochthonous communities, researching the concept of 'otherness' taken from Post-colonial theories. It is for this purpose, that my methodologies include self-reflection, parallelism and metaphor. One of my purposes is toreveal those 'fake stories' used by the European acculturation in South America. I called these fake stories 'strategies', as they were used by the Colony for the reconstruction of a new National Identity in those countries. Therefore, convincing the Indigenous culture to adopt Western culture. The parts of my essay are a metaphor of different stages of narcotic's production, best called 'mobile labs' of the Amazon jungle.I have taken this concept to tell my story and to build up the laboratory as final representation. The first stage of this process is to weigh and measure the ingredients, followed by a mixture of substances and chemicals. Once mixed, it proceeds to three stages of filtering and purification. Then it is subjected to a press for semi-solid consistency and introduced into the oven to reach the compact state. The blocks will finally be packed with plastic film and adhesive tape, protecting them from adverse environmental conditions during transport and storage. Each chapter of this essay is also connected to the 'machines' constructed for my solo exhibition, following the same steps of Purification. Through this essay, I wanted to broaden my concept relating three main aspects: the colonial strategies of domination in South America, my personal work experience in Europe, and finally the unfair working conditions in marginal societies. Each of the 'machines' constructed for my installation is functional, ready to be activated according to the different parts of the cooking process and as a representation of a 'production line' in the system of labor. I also have chosen to wrap my body, as well as carefully chosen representative objects of the popular culture of South America, to finally pack them in cling film. As a result of this process of Purification, I have produced the 'final products' ready to be consumed here in the Western European countries.
8

Aufy, A. A. S. M. "PROTEIN PURIFICATION FROM GASTROPODS." Doctoral thesis, Università degli Studi di Milano, 2011. http://hdl.handle.net/2434/152912.

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Gastropods are a class of invertebrates within the mollusks, commonly known as snails and slugs. Recently, scientific interest is directed on snails as pollution indicators, as destroyers of crops, but also as parasites carriers. Furthermore, some species of snails are considered as an important human food source in countries like France and Australia. In scientific research, snails are used as model animals especially in molecular biology and immunology. Some snails secrete purple matter with anti-cancer property; the snails use it to protect their eggs and scientists hope to create with it a weapon against breast cancer. In the light of this, the aim of the present work was to purify and characterize exoglycosidases, i.e. sugars-hydrolyzing enzymes, extracted from different species of snails and to study the glycosylation pattern of their tissues by using lectins as glycoprotein-specific antibodies. Purification work started with the screening of 7 different exoglycosidases (α-fucosidase, β-xylosidase, α-mannosidase, β-mannosidase, α-glucosidase, β-glucosidase, β- galactosidase and β-N-acetylglucosaminidase) in 8 different species of snails (Arion lusitanicus, Biomphalaria glabrata, Achatina fulica, Limax maximus, Cepaea hortensis, Lymnea stagnalis, Arianta arbustorum, Planorbarius corneus and egg from Achatina fulica). In order to create a purification scheme, many trials have been performed on the chosen enzyme, i.e. β-galactosidase from Arion lusitanicus. Ten snails were carefully washed after complete removal of the abdomen content to eliminate other sources of the enzyme. Snails were then homogenized and protein was precipitated by 1.2 M ammonium sulfate under cooling centrifugation. Then the precipitate was fractionated by the column hydrophobic interaction chromatography. Fractions exhibiting high enzyme activity were pooled, concentrated, desalted by ultrafiltration and then applied to affi gel blue. As the use of affi gel blue resulted in no binding between the enzyme and the gel beads, the unbound fractions were directly applied to anion exchange chromatography. As this procedure behaved as the same way of affi gel blue, the unbound fractions were pooled, concentrated to less than 2 ml and applied to size exclusion chromatography. Size exclusion chromatography exhibited high resolution purification, because a narrow peak was obtained, after testing the eluate for the enzyme activity. The fractions within this narrow peak were then applied to cation exchange chromatography, resulting in two new very sharp peaks. Fractions of the second peak from cation exchange chromatography were exposed to SDS-PAGE that indicated high quality purification, because just two proteins’ bands appeared. Finally, β-galactosidase specific affinity was the last purification step, where four different buffers were used (50mM sodium citrate pH 4.6, 1M NaCl in50 mM sodium citrate pH 4.6, 50 mM Tris/HCl pH 7.5 and 1M NaCl in 50 mM Tris/HCl pH 7.5) and the enzyme was eluted only with 50 mM Tris/HCl pH 7.5 buffer. Electrophoresis of this eluate resulted in a single protein band in one fraction, identified by LC-MS as galactocerebrosidase with molecular weight about 74 kDa. β-galactosidase characterization tests indicated that the optimal enzyme activity was at pH range 3.5 to 5.0 regardless to the used salt, i.e. the enzyme needs no special cations to be active, and indicated that the maximum activity of the enzyme is reached after 4 h incubation at 37 °C. β-galactosidase purification from species other than Arion lusitanicus resulted in different purified proteins but not the β-galactosidase. Moreover, as fucose and sialic acid are frequent and common modifications in snail glycans and they occur in a variety of different linkages and may therefore contribute to a number of recognition and adhesion processes, the glycosylation patterns of snails’ tissue were studied by different lectins, i.e. glycoproteins specific antibodies. We analysed eggs and adult land snails and water snails (Achatina fulica, Arion lusitanicus, Arianta arbustorum, Biomphalaria glabrata, Cepaea hortensis, Limax maximus, Lymnea stagnalis, Planorbarius corneus) for their N- and O-glycosylation pattern with a focus on their sialylation and fucosylation abilities. Their sialylation potential was investigated by Sambucus nigra agglutinin and Maackia amurensis agglutinin while their fucosylation potential was investigated by Aleuria aurantia lectin, Lens culinaris agglutin, Lotus tetragonolobus agglutinin and Ulex europaeus agglutinin before and after tissues’ digestion with glycopeptidase F and β-elimination, respectively. In conclusion, 1) β-galactosidase purification from Arion lusitanicus needs several purification steps and must be conducted with specific β-galactosidase affinity chromatography, otherwise several unwanted proteins will appear as co-purified proteins. 2) The same purification scheme of Arion lusitanicus, when adopted in other snails, did not result in purifying β-galactosidase. 3) From the lectin study, it was confirmed that fucose and sialic acid are frequent and common modifications in snail glycans.
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Blom, Hans. "Purification Processes for Complex Biomacromolecules." Doctoral thesis, Uppsala universitet, Fysikalisk-organisk kemi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-172892.

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This thesis details various techniques and considerations for the purification of complex biomacromolecules.   Initially an α-mannosidase from babaco fruit was purified using anion exchange-, lectin affinity- and size exclusion chromatography.  The enzyme was approximately 260-280 kDa in size with an apparent an unusual octagonal stoichiometry and displayed properties similar to other known plant α-mannosidases.   Mucins were fractionated by ion exchange and size exclusion chromatography to assess the properties that govern the mucin surface coating interactions in biomaterial research.  Commercially available mucins, of bovine and porcine origin, as wells as crude human mucin were tested. All showed to consist of a population of molecules which differ in size, charge and composition.   The third part of the thesis concerns different aspects of plasmid DNA purification processes. A two-step method for analysis of plasmid DNA consisting of size exclusion followed by thiophilic adsorption chromatography was evaluated. It allowed determination of the supercoiled plasmid DNA concentration in all process steps without requirement for extensive sample preparation. This method was shown to be fully comparable in terms of accuracy to capillary gel electrophoresis, considered as the industry standard. Purification of plasmid DNA generally involves bacterial cell alkaline lysis, which creates a solution with flocculate material which needs to be removed prior to further processing. The addition of ammonium hydrogen carbonate to the suspension was evaluated to clarify the solution. The released carbon dioxide and ammonium lifts the flocculate to the surface and allows draining of a clear solution. The method is fully scalable, does not affect the plasmid DNA quality and requires no special equipment. Thiophilic adsorption chromatography was evaluated for simplification of an existing commercial large scale purification process and was shown to increase both product purity and yields of several tested plasmids. Also, implementation of this step significantly reduced overall production process time.
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Leah, Labib. "Helicase Purification for DNA Sequencing." Thesis, Université d'Ottawa / University of Ottawa, 2014. http://hdl.handle.net/10393/31341.

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BACKGROUND: A method to increase accuracy and ease-of-use, while decreasing time and cost in deoxyribonucleic acid (DNA) sequence identification, is sought after. Helicase, which unwinds DNA, and avidin, which strongly attracts biotin for potential attraction of biotinylated DNA segments, were investigated for use in a novel DNA sequencing method. AIM: This study aimed to (1) purify bacteriophage T7 gene product 4 helicase and helicase-avidin fusion protein in a bacterial host and (2) characterize their functionality. METHODS: Helicase and helicase-avidin were cloned for purification from bacteria. Helicase-avidin was solubilised via urea denaturation/renaturation. DNA and biotin binding were assessed using Electrophoretic Mobility Shift Assays and biotinylated resins, respectively. RESULTS: (1) Helicase and helicase-avidin proteins were successfully purified. (2) Helicase protein was able to bind DNA and avidin protein strongly bound biotin. CONCLUSION: Helicase and helicase-avidin can be purified in a functional form from a bacterial host, thus supporting further investigation for DNA sequencing purposes.
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Книги з теми "Purification":

1

Bonner, Philip L. R. Protein Purification. Second edition. | Boca Raton : Taylor & Francis, 2018. | Series: Basics: Taylor & Francis, 2018. http://dx.doi.org/10.1201/9780429458187.

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2

Litwack, Gerald, ed. Receptor Purification. Totowa, NJ: Humana Press, 1990. http://dx.doi.org/10.1007/978-1-4612-0461-9.

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3

Litwack, Gerald, ed. Receptor Purification. Totowa, NJ: Humana Press, 1990. http://dx.doi.org/10.1007/978-1-4612-0477-0.

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4

Scopes, Robert K. Protein Purification. New York, NY: Springer New York, 1987. http://dx.doi.org/10.1007/978-1-4757-1957-4.

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5

Scopes, Robert K. Protein Purification. New York, NY: Springer New York, 1994. http://dx.doi.org/10.1007/978-1-4757-2333-5.

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6

Ladisch, Michael R., Richard C. Willson, Chih-duen C. Painton, and Stuart E. Builder, eds. Protein Purification. Washington, DC: American Chemical Society, 1990. http://dx.doi.org/10.1021/bk-1990-0427.

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7

Kohl, Arthur L. Gas purification. 5th ed. Houston, Tex: Gulf Pub., 1997.

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8

Janson, Jan-Christer, ed. Protein Purification. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2011. http://dx.doi.org/10.1002/9780470939932.

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9

Nikolaj, Gertsen, and Sønderby Linus, eds. Water purification. Hauppauge, N.Y: Nova Science Publishers, 2009.

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10

Gerald, Litwack, ed. Receptor purification. Clifton, N.J: Humana Press, 1990.

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Частини книг з теми "Purification":

1

Van Meter, Rodney. "Purification." In Quantum Networking, 175–94. Chichester, UK: John Wiley & Sons, Ltd, 2014. http://dx.doi.org/10.1002/9781118648919.ch9.

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2

Morris, Stephen. "Purification." In The New Palgrave Dictionary of Economics, 11050–54. London: Palgrave Macmillan UK, 2018. http://dx.doi.org/10.1057/978-1-349-95189-5_2679.

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3

Morris, Stephen. "Purification." In The New Palgrave Dictionary of Economics, 1–6. London: Palgrave Macmillan UK, 2008. http://dx.doi.org/10.1057/978-1-349-95121-5_2679-1.

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4

Ron, Nathan. "Purification." In Erasmus and the “Other”, 141–45. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-24929-8_10.

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5

Durlauf, Steven N., and Lawrence E. Blume. "Purification." In Game Theory, 279–85. London: Palgrave Macmillan UK, 2010. http://dx.doi.org/10.1057/9780230280847_29.

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6

Bährle-Rapp, Marina. "purification." In Springer Lexikon Kosmetik und Körperpflege, 461. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_8651.

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7

Gallaher, Timothy K., and Howard H. Wang. "Serotonin Receptors." In Receptor Purification, 1–21. Totowa, NJ: Humana Press, 1990. http://dx.doi.org/10.1007/978-1-4612-0461-9_1.

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8

Dattatreyamurty, Bosukonda, and Leo E. Reichert. "The Follicle Stimulating Hormone Receptor." In Receptor Purification, 191–215. Totowa, NJ: Humana Press, 1990. http://dx.doi.org/10.1007/978-1-4612-0461-9_10.

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9

Akamizu, Takashi, Michele De Luca, and Leonard D. Kohn. "Thyrotropin Receptor." In Receptor Purification, 217–48. Totowa, NJ: Humana Press, 1990. http://dx.doi.org/10.1007/978-1-4612-0461-9_11.

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10

Leedman, Petu J., and Leonard C. Harrison. "Thyrotropin Receptor Purification." In Receptor Purification, 249–68. Totowa, NJ: Humana Press, 1990. http://dx.doi.org/10.1007/978-1-4612-0461-9_12.

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Тези доповідей конференцій з теми "Purification":

1

Kishimoto, Y. "Liquid Scintillator Purification." In TOPICAL WORKSHOP ON LOW RADIOACTIVITY TECHNIQUES: LRT 2004. AIP, 2005. http://dx.doi.org/10.1063/1.2060471.

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2

Reimann, Oliver, Dominik Sarma, Robert Zitterbart, Gavin Noble, and Oliver Seitz. "Enabling Parallel Peptide Purification by a Novel Traceless Purification Linker." In 35th European Peptide Symposium. Prompt Scientific Publishing, 2018. http://dx.doi.org/10.17952/35eps.2018.021.

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3

Christensen, Henrik, Jørgen Dinesen, Henriette Hare Engell, Lars Christian Larsen, Kent Kammer Hansen, and Eivind M. Skou. "Electrochemical Exhaust Gas Purification." In SAE 2000 World Congress. 400 Commonwealth Drive, Warrendale, PA, United States: SAE International, 2000. http://dx.doi.org/10.4271/2000-01-0478.

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4

SUDARSHAN, E. C. G. "DECOHERENCE, PURIFICATION AND ENTANGLEMENT." In Proceedings of the XXII Solvay Conference on Physics. WORLD SCIENTIFIC, 2003. http://dx.doi.org/10.1142/9789812704634_0005.

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5

Kuźniak, Marcin. "Acrylic purification and coatings." In TOPICAL WORKSHOP ON LOW RADIOACTIVITY TECHNIQUES: LRT-2010. AIP, 2011. http://dx.doi.org/10.1063/1.3579566.

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6

Giammarchi, M., M. Balata, A. Goretti, A. Ianni, L. Ioannucci, L. Miramonti, and S. Nisi. "Water purification in Borexino." In LOW RADIOACTIVITY TECHNIQUES 2013 (LRT 2013): Proceedings of the IV International Workshop in Low Radioactivity Techniques. AIP, 2013. http://dx.doi.org/10.1063/1.4818110.

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7

Soni, Apurva, Kushagra Singh, and Praveen Kumar. "Smart Water Purification Technique." In 2020 2nd International Conference on Advances in Computing, Communication Control and Networking (ICACCCN). IEEE, 2020. http://dx.doi.org/10.1109/icacccn51052.2020.9362834.

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8

Wang, Bing. "Purification of fumarated rosin." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/iywn7624.

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Fumarated rosin can be manufactured by Diel-Alder reaction of rosin, a by-product from kraft paper pulping method. There is always about 50% unreacted rosin left in the process. Recently, there is renewed demand for higher purity of fumarated rosin, due to its multifunctional character. We have developed a new and scalable method to increase the purity of fumarated rosin. We will be discussing the details of this inventive method.
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Singh, Himanshu, and A. V. Subramanyam. "Language Guided Adversarial Purification." In ICASSP 2024 - 2024 IEEE International Conference on Acoustics, Speech and Signal Processing (ICASSP). IEEE, 2024. http://dx.doi.org/10.1109/icassp48485.2024.10446676.

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10

Freeman, Eric, Lisa Mauck Weiland, and Ryan Soncini. "Water Purification Through Selective Transport." In ASME 2011 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. ASMEDC, 2011. http://dx.doi.org/10.1115/smasis2011-5062.

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Accumulation of inorganic nitrates and phosphates in regions such as the Mississippi river basin has resulted in catastrophic growth of algal blooms. These algal blooms deplete the surrounding oxygen and asphyxiate nearby aquatic life, resulting in large regions incapable of sustaining life. Using biomimicry principles to design a tailored active material to selectively transport these pollutants may offer a strategy to restore these dead zones to health. Theoretically a combination of selected protein transporters may be employed to create a selective sponge to reclaim these nitrates and phosphates. Presented is a feasibility study of various configurations of transporters, and a unique solution for restoring the aquatic ecosystem back to health.

Звіти організацій з теми "Purification":

1

Baker, J. D. Polonium purification. Office of Scientific and Technical Information (OSTI), September 1996. http://dx.doi.org/10.2172/385614.

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2

Majumdar, S., A. K. Guha, Y. T. Lee, T. Papadopoulos, and S. Khare. Liquid membrane purification of biogas. Office of Scientific and Technical Information (OSTI), March 1991. http://dx.doi.org/10.2172/5065322.

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3

Bartscherer, K. A., J. J. de Pablo, M. C. Bonnin, and J. M. Prausnitz. Purification of aqueous cellulose ethers. Office of Scientific and Technical Information (OSTI), July 1990. http://dx.doi.org/10.2172/6084196.

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4

Pegah Hassanzadeh, Pegah Hassanzadeh. Water Purification for Developing Countries. Experiment, August 2014. http://dx.doi.org/10.18258/3218.

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5

Tepper, Gary. The SELEX Air Purification System. Fort Belvoir, VA: Defense Technical Information Center, January 2010. http://dx.doi.org/10.21236/ada518955.

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6

Wu, J. Argon Purification Reference and Recommendation. Office of Scientific and Technical Information (OSTI), May 1991. http://dx.doi.org/10.2172/1031767.

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7

Goodell, Margaret, and Jeffrey Rosen. Purification of Mammary Gland Stem Cells. Fort Belvoir, VA: Defense Technical Information Center, September 2001. http://dx.doi.org/10.21236/ada397791.

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8

Thompson, M. C., and R. A. Pierce. Purification Testing for HEU Blend Program. Office of Scientific and Technical Information (OSTI), June 1998. http://dx.doi.org/10.2172/676767.

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9

Stote, Robert E. Protocol for Initial Purification of Bacteriocin. Fort Belvoir, VA: Defense Technical Information Center, October 2015. http://dx.doi.org/10.21236/ada627577.

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10

Leppla, Stephen H. Production and Purification of Anthrax Toxin. Fort Belvoir, VA: Defense Technical Information Center, May 1986. http://dx.doi.org/10.21236/ada170131.

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