Готові списки джерел за темами / Pure culture

Добірка наукової літератури з теми "Pure culture"

Автор: Grafiati
Опубліковано: 14 березня 2023

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Статті в журналах з теми "Pure culture"

1

Kåhrström, Christina Tobin. "An impure “pure culture”." Nature Reviews Microbiology 11, no. 5 (April 16, 2013): 301. http://dx.doi.org/10.1038/nrmicro3021.

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2

Agius, L. "Metabolic interactions of parenchymal hepatocytes and dividing epithelial cells in co-culture." Biochemical Journal 252, no. 1 (May 15, 1988): 23–28. http://dx.doi.org/10.1042/bj2520023.

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When parenchymal hepatocytes isolated from adult liver are co-cultured with other epithelial cells, the production of various plasma proteins by the hepatocytes is preserved for much longer than in conventional culture. This study examines some of the metabolic interactions between parenchymal hepatocytes and epithelial cells maintained in co-culture. The leakage of lactate dehydrogenase by hepatocytes co-cultured with epithelial cells was lower than in conventional hepatocyte culture. The epithelial cells have a high glycolytic rate and provide the hepatocytes with a continual supply of lactate. The [lactate] was lower in co-cultures of hepatocytes and epithelial cells than in pure epithelial cultures of similar density, suggesting lactate clearance by the hepatocytes. Alanine uptake was higher in conventional hepatocyte cultures, which lack an exogenous supply of lactate, than in parenchymal hepatocytes in co-culture. Studies with pure parenchymal hepatocytes incubated with increasing [lactate] suggest that lactate is utilized in preference to alanine as a gluconeogenic substrate by hepatocytes co-cultured with epithelial cells. Ketogenesis and carnitine palmitoyltransferase activity declined more slowly in hepatocytes co-cultured with epithelial cells than in conventional culture. It is concluded that the co-culture model has potential for long-term studies of carbohydrate and lipid metabolism.
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3

Koveshnikov, V. S., M. A. Matvienko та A. A. Rodionova. "Starter culture for kоumiss from pure dry bacterial cultures". Dairy Industry 65, № 9 (2020): 52–54. http://dx.doi.org/10.31515/1019-8946-2020-9-52-54.

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4

Hutchison, Leonard J. "Notes onLimacella Illinitain Pure Culture." Mycologia 80, no. 1 (January 1988): 111–14. http://dx.doi.org/10.1080/00275514.1988.12025507.

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5

Kostina, O. V. "On «Pure» Conditions in Culture." Philosophy. Psychology. Pedagogy 15, no. 2 (April 27, 2015): 8–13. http://dx.doi.org/10.18500/1819-7671-2015-15-2-8-13.

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6

Roldán, A., E. Descals, and M. Honrubia. "Pure culture studies on Tetracladium." Mycological Research 93, no. 4 (December 1989): 452–65. http://dx.doi.org/10.1016/s0953-7562(89)80039-5.

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7

Hasanuddin, Hasanuddin. "APLIKASI STARTER KULTUR MURNI PADA PEMBUATAN DURIAN FERMENTASI." Jurnal Agroindustri 1, no. 2 (November 25, 2011): 74–80. http://dx.doi.org/10.31186/j.agroind.1.2.74-80.

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This study looks into the effect of pure culture starters isolated from tempoyak on fermented durian (Durio zibethinus) and its acceptability. The use of 5% pure culture starters and mixed cultures of Pediococcus acidilactici, Lactobacillus plantarum, Lactobacillus curvatus Leuconostoc mesentroides and Staphylococcus saprophyticus were analyzed in terms of acceptabilty by sensory panelists. Statistical analysis shows a significant difference among fermented durian with pure culture and mixed culture starters for attributes of texture and viscosity, aroma and flavor, acidity and general acceptability.
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8

Molokwanne, P. E., and E. M. N. Chirwa. "Biological Cr(VI) reduction in indigenous sludge cultures from Gauteng, South Africa." Water Science and Technology 54, no. 10 (November 1, 2006): 177–84. http://dx.doi.org/10.2166/wst.2006.880.

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The Cr(VI) reducing capability of an acclimated indigenous culture cultivated from primary sludge was evaluated in batch and packed-bed bioreactor systems. Performance evaluation was carried out in unmodified cultures, cultures modified by substituting terminal organisms in the consortium by a known Cr(VI)-reducing organism (Escherichia coli ATCC 33456), and pure cultures of Cr(VI)-reducing organisms. A high Cr(VI) reduction rate was observed in modified cultures and in the pure culture of the Cr(VI)-reducing bacteria (Bacillus sp.). Furthermore, the Bacillus sp. pure culture outperformed both the unmodified and modified consortium cultures in reducing Cr(VI). Abiotic Cr(VI) reduction activity was evaluated in heat-killed and azide (N3−) inactivated control cultures. No significant Cr(VI) reduction was observed in the controls. This study is part of the continuing research to identify synergistic culture systems for treating toxic compounds from polluted environments.
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9

Reyniers, James A. "THE PURE-CULTURE CONCEPT AND GNOTOBIOTICS." Annals of the New York Academy of Sciences 78, no. 1 (December 15, 2006): 3–16. http://dx.doi.org/10.1111/j.1749-6632.1959.tb53091.x.

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10

Lebreton, J. P., M. Daveau, M. Hiron, M. Fontaine, D. Biou, D. Gilbert та C. Guguen-Guillouzo. "Long-term biosynthesis of complement component C3 and α-1 acid glycoprotein by adult rat hepatocytes in a co-culture system with an epithelial liver cell-type". Biochemical Journal 235, № 2 (15 квітня 1986): 421–27. http://dx.doi.org/10.1042/bj2350421.

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We used a system of co-culture of adult rat hepatocytes with another epithelial cell type from rat liver to study the synthesis of two acute-phase reactants, alpha-1 acid glycoprotein (alpha 1AGP) and the third component of complement (C3), and we have obtained long-term secretion of these two proteins. After a period of adaptation corresponding to the first 2-4 days of the co-culture, hepatocytes secreted C3 and alpha 1AGP for at least 2 weeks at a mean level higher than that observed in the first days of a pure culture of hepatocytes. When pulse-chase analysis was performed on day 6 of co-culture, kinetics of synthesis of alpha 1AGP and C3 were the same as those observed on day 1 of a conventional culture of pure hepatocytes. Furthermore, intracellular and extracellular alpha 1AGP had Mr values respectively of 39,000 and of 42,000-52,000, identical with those observed in pure cultures of hepatocytes. Similarly, the molecular size and subunit structures of C3 were the same in co-culture and in cultures, indicating an identical processing of this protein. C3 produced in co-culture was also haemolytically active. Therefore, the system of adult hepatocytes co-cultured with this liver epithelial cell provides a physiological system in vitro which permits long-term synthesis of the two acute-phase reactants C3 and alpha 1AGP. This model opens the possibility to study the modulation of the synthesis of these two proteins during a long period by inflammatory agents or by hormones.
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Дисертації з теми "Pure culture"

1

Porcu, Elisabetta. "Pure Land Buddhism in modern Japanese culture /." Leiden : Brill, 2008. http://catalogue.bnf.fr/ark:/12148/cb413311546.

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2

MAZURE, NATHALIE. "Etude de la biodegradation de la morphine par une culture pure mycobacterium aurum, et les cultures mixtes, pseudomonas." Compiègne, 1993. http://www.theses.fr/1993COMP648S.

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La morpholine est un compose heterocyclique sature. C'est une base forte et ses proprietes de solvant en font un compose couramment utilise dans l'industrie chimique. Compose xenobiotique, la morpholine a ete classee pendant longtemps comme tres persistante. Cependant, l'isolement recent de cultures capables de la degrader l'a sortie definitivement des listes des composes non biodegradables. Les connaissances sur la biodegradabilite de la morpholine se resume en une voie de degradation hypothetique etablie a partir de mycobacterium chelonae. Dans ce travail, nous avons choisi d'aborder l'etude de la degradation de la morpholine en menant de front la microbiologie et la biologie moleculaire de deux populations, l'une pure (mycobacterium aurum: gram-positif) et l'autre mixte, essentiellement composee de pseudomonas (gram-negatif). Avec m. Aurum, nous avons etabli ses capacites degradatives et l'interet de l'immobilisation et observe l'existence d'une gangue en presence de morpholine, ainsi que d'un mecanisme de regulation des deux branches de la voie de degradation. Les genes de la morpholine ne s'exprimant pas ou peu dans e. Coli le392, des mutants mor- et eta- ont ete obtenus. La culture mixte nous a permis de suivre l'evolution d'une culture apres deux annees de degradation dans une station d'epuration et d'observer l'augmentation de ses capacites degradatives. Aucun phenotype relatif a la degradation n'a pu etre impute a la presence de grands plasmides dans l'une et l'autre des cultures malgre les echanges genetiques observes. Ces deux cultures ont montre que l'ouverture du cycle se faisait au niveau de liaison c-n et que la presence d'une double liaison l'empechait. Il semble que dans les cas, les genes soient chromosomiques
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3

Zarnegar, Abdolreza. "Purification and Characterization of an Inhibitor of Thymidine Uptake From Culture Supernatants of Human Tonsil Lymphocytes." Digital Commons @ East Tennessee State University, 1987. https://dc.etsu.edu/etd/2833.

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Lymphocytes from human tonsils were cultured in the absence of serum for 3 days. In the presence of the concentrated culture supernatant the proliferative response of PBL, to con A, as measured by the uptake of ('3)H-tdr, was significantly reduced. The suppressor substance was referred to as SMAL (suppressor of mitogen activated lymphocytes). The estimated molecular weight of SMAL under nondenaturing conditions was 100,000-300,000. SMAL also suppressed the incorporation of ('3)H-tdr by a variety of mouse and human tumor cell lines. The activity of SMAL was sensitive to pronase and heating at 100(DEGREES)C for 30 minutes but insensitive to RNase. Treatment with DNase, however, enhanced the activity of SMAL. SMAL activity was also destroyed by treatment with 5% TCA, 0.4 M HCl or 60% acetonitrile, but resistant to 6 M urea or dialysis against pH 2 buffer for 24 hours. SMAL activity was precipitated in 40-80% ammonium sulfate saturation. When applied to a phenyl-sepharose column no activity was recovered. SMAL was not produced by heat-killed tonsil lymphocytes or lymphocytes-treated with cycloheximide. Maximal production occurred in the first 24 hours of culture, and progressively less was produced in subsequent 24-hour intervals. Both T- and B lymphocyte-enriched culture supernatants contained SMAL. SMAL adhered strongly to DEAE-cellulose, but less than two-fold purification was achieved. Using QMA-Accell anion exchange medium, a 5-fold purification of SMAL with higher specific activity was obtained with HPLC. Activity of SMAL was recovered after native polyacrylamide gel electrophoresis by electroblotting to DEAE-cellulose paper followed by eluting the bound materials with salt. Two active components, one corresponding to a large and/or less negatively charged molecule and another corresponding to a small and/or highly acidic molecule, were recovered. HPLC-purified SMAL at relatively low doses inhibited the uptake and phosphorylation of ('3)H-tdr, without significant effect on cell proliferation. The inhibition of ('3)H-tdr uptake was favored over that of ('3)H-udr or ('3)H-adr, and this effect was reversible. At relatively high doses of HPLC-purified SMAL, the growth of mouse thymoma EL-4 and human T cell leukemia CEM-CM(,3) cell lines was inhibited.
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4

Brading, Melanie Gayle. "The influence of fluid dynamics and surface material on pure and binary culture biofilms." Thesis, University of Exeter, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307314.

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5

Wang, Hao. "Development and electrochemical characterization of a Pseudomonas aeruginosa-based pure culture microbial fuel cell." University of Dayton / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1311958719.

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6

Lamballe, Fabienne. "Reparation du dna dans les hepatocytes de rat adulte en culture pure et co-culture : mecanismes enzymatiques et expression d'oncogenes nucleaires." Rennes 1, 1989. http://www.theses.fr/1989REN10038.

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Deux modeles experimentaux disponibles: la culture pure d'hepatocytes de rat et la co-culture lorsque ces derniers sont associes avec les cellules epitheliales hepatiques. Dans les deux systemes etudies, deux dna ligases distinctes de part leurs proprietes catalytiques ont ete identifiees. Apres irradiation uv, seule la ligase ii est stimulee. Cette enzyme semble par consequent etre impliquee dans la reparation du dna. Cette stimulation correspond a une synthese de novo, mais il semble n'y avoir activation de la transcription que dans le modele de co-culture. Apres irradiation , les dna polymerases et ont stimulees en culture pure alors qu'en co-culture, seule la polymerase est activee. Apres irradiation uv, seule la polymerase semble etre activee. L'etude de l'expression d'oncogenes nucleaires montre que c-myc est stimule apres irradiation des cellules alors que l'expression de c-myb ne semble pas etre modifiee
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7

Bally, Matthias. "Physiology and ecology of nitrilotriacetate degrading bacteria in pure culture, activated sludge and surface waters /." [S.l.] : [s.n.], 1994. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=10821.

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Qiu, Yu-Shan. "Development of a successful method for quantifying viable oral anaerobic spirochetes from pure culture and periodontal pockets." Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=68246.

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Spirochetes are markedly prevalent in periodontal disease but are not included as predominant cultivable organisms because of the inability to quantify them by viable count. A successful method was developed for enumerating viable oral spirochetes in pure culture as colony-forming units (CFU) in new oral spirochete (NOS) medium with 0.7% agarose and using small tissue-culture flasks. Three species of oral spirochetes in log-phase growth in NOS broth were used for evaluation of the method. Reliable, consistent and reproducible viable counts of pure spirochete cultures were obtained.
This method was extended to enumerate viable oral spirochetes from periodontal pockets. The antibiotic rifampin (20 $ mu$g/ml) was found to be an excellent selective agent for such a count when added to NOS-agarose medium. Counts of cultivable oral spirochetes from 10 subgingival plaque samples ranged from 12.5% to 28.2% of the total cultivable anaerobic bacteria. In addition, by the use of this method thirteen new oral spirochetes were isolated.
The viable count technique was modified and employed to study the locomotion of spirochetes. Migration of oral spirochetes out of NOS-Bacto agar medium into NOS-agarose medium was observed and two locomotory phenotypes of oral spirochetes were detected.
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9

Kijkla, Pruch. "Biocide Mitigation of Carbon Steel and Stainless Steel Biocorrosion by Pure-Strain and Mixed-Culture Microbial Biofilms." Ohio University / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1619007982435067.

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Bertrand, François. "Les oidiums des cucurbitacees : maintien en culture pure, etude de leur variabilite et de la sensibilite chez le melon." Paris 11, 1991. http://www.theses.fr/1991PA112286.

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Des isolats monoconidiens des oidiums des cucurbitacees (sphaerotheca fuliginea et erysiphe cichoracearum, en abrege s. F. Et e. C. ) sont maintenus en culture pure sur de jeunes cotyledons de concombre excises places sur un milieu saccharose-mannitol gelose. In vitro, la sporulation est reduite a l'obscurite ou par inhibition chimique de la photosynthese, accrue par apport de glucides ou de glycerol, legerement accrue par le mannitol, inhibee par l'inositol. S. F. Et e. C. Different par leur comportement en cas d'absence de photoperiode, d'augmentation d'eclairement, de baisse d'humidite relative et par leur germination sur de l'eau gelosee. Sur 147 echantillons de plantes parasitees collectes de 1987 a 1989, s. F. Domine en ete, e. C. Est plus frequent au printemps et sous abris. Des pathotypes se distinguent sur concombre (a) cv. Marketer, melon (b) cv. Vedrantais (1) et p. M. R. 45 (2), courgette (c) cv. Diamant et pasteque (d) cv. Sugar baby. Les pathotypes de e. C. (a, ab(1,2), ac et acd) sont ubiquistes. Pour s. F. , le pathotype a est seul trouve dans le nord de la france, ab(1,2)c domine dans le sud. Les clones de s. F. Se classent en 2 groupes de compatibilite sexuelle complementaires; 86 clones produisent peu de cleistotheces, 5 sont tres productifs. Chez e. C. , les clones a sont sexuellement compatibles avec les clones ac et acd, les clones ab ne forment pas de cleistotheces. Sur 456 genotypes de melon, 4 sont sensibles a s. F. A et e. C. A, 151 sont resistants a e. C. Ab et sensibles a s. F. Abc, 35 sont resistants a s. F. Et sensibles a e. C. Ab. La resistance a s. F. Ab(1,2)c n'est jamais trouvee sans la resistance a s. F. Ab(1)c
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Книги з теми "Pure culture"

1

Pure Land Buddhism in modern Japanese culture /c by Eisabetta Porcu. Leiden: Brill, 2008.

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2

Steinberg, Deborah Lynn. Pure culture: A feminist analysis of IVF ethos and innovation. Birmingham: University of Birmingham, 1992.

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3

Michael, Aris, ed. High peaks, pure earth: Collected writings on Tibetan history and culture. London: Serindia Publications, 1998.

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4

Freeman, Dale. How to talk pure Ozark in one easy lesson. Kimberling City, MO (P.O. Box 550, Kimberling City 65686): Ozark Postcard Publishers, 1991.

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5

Ŭi-bŏm, Wŏn. A history of Korean Buddhist culture and some essays: The Buddhist Pure Land & the Christian Kingdom of Heaven. Seoul, Korea: Jip Moon Dang Pub., 1992.

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6

Richardson, John Adkins. From pure visibility to virtual reality in an age of estrangement. Westport, Conn: Praeger, 1998.

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7

Définitions de la culture visuelle (2nd 1995 Montréal, Québec). Définitions de la culture visuelle II: Utopies modernistes, postformalisme et purete de la vision = Definitions of visual culture II : modernist utopias, postformalism and pure visuality. Montréal: Musee d'art contemporain de Montréal, 1996.

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8

The word on the street: Debunking the myth of "pure" standard English. Cambridge, Mass: Perseus Pub., 2000.

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9

Neilson, James. Attachment of bacteria to glass surfaces in pure culture and in mixed suspensions and the effect of growth conditionson that attachment. [s.l.]: typescript, 1991.

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10

Beyond pure reason: Ferdinand de Saussure's philosophy of language and its early romantic antecedents. New York: Columbia University Press, 2012.

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Частини книг з теми "Pure culture"

1

Gooch, Jan W. "Pure Culture." In Encyclopedic Dictionary of Polymers, 918. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_14617.

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2

Monahan, Barry. "Pure Male: Masculine Spaces and Stasis in Eugene O’Brien’s Pure Mule (2005)." In Masculinity and Irish Popular Culture, 207–20. London: Palgrave Macmillan UK, 2014. http://dx.doi.org/10.1057/9781137300249_16.

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3

Chua, Liana. "The Making of a “Not Yet Pure Christian” Village." In The Christianity of Culture, 81–105. New York: Palgrave Macmillan US, 2012. http://dx.doi.org/10.1057/9781137012722_4.

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4

Hamra, F. Kent, Karen M. Chapman, Zhuoru Wu, and David L. Garbers. "Isolating Highly Pure Rat Spermatogonial Stem Cells in Culture." In Methods in Molecular Biology™, 163–79. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-214-8_12.

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Ernst, Stephen G. "In vitro culture of pure species non-aspen poplars." In Micropropagation of Woody Plants, 195–207. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-015-8116-5_12.

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Rosenbaum, Miriam A., Carola Berger, Simone Schmitz, and Ronny Uhlig. "Microbial Electrosynthesis I: Pure and Defined Mixed Culture Engineering." In Bioelectrosynthesis, 181–202. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/10_2017_17.

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Itoh, Mayumi. "Pure Land Buddhism and Whaling Culture in the Chūgoku Region." In The Japanese Culture of Mourning Whales, 115–43. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-6671-9_7.

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8

Beloqui, A., M. E. Guazzaroni, and M. Ferrer. "Procedures for Protein Isolation in Pure Culture and Microbial Communities." In Handbook of Hydrocarbon and Lipid Microbiology, 4183–94. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-77587-4_326.

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9

Rhodes, Neil. "Pure and Common Greek in Early Tudor England." In The Culture of Translation in Early Modern England and France, 1500–1660, 54–70. London: Palgrave Macmillan UK, 2015. http://dx.doi.org/10.1057/9781137401496_4.

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10

Kuo, Chung-Shen, Wenliang Lu, and Yao-Lin Kui. "Corn (Zea mays L.): Production of Pure Lines Through Anther Culture." In Biotechnology in Agriculture and Forestry, 168–80. Berlin, Heidelberg: Springer Berlin Heidelberg, 1986. http://dx.doi.org/10.1007/978-3-642-61625-9_10.

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Тези доповідей конференцій з теми "Pure culture"

1

ABDULLA, Diar N., and Bahaa A. SHIHAB. "THE ANTIFUNGAL EFFECT OF CRUD ALCOHOLIC EXTRACT OF ANCHUSA STRIGOSA ON T. PEDIS." In IV.International Scientific Congress of Pure,Appliedand Technological Sciences. Rimar Academy, 2022. http://dx.doi.org/10.47832/minarcongress4-32.

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Tinea pedis is the most common type of fungal infection in human. The samples have been taken from fifty patients (26 female, 24 male) their ages ranged from 11 to over 60 years diagnosed as having Tinea pedis in Baghdad Teaching Hospital. All samples were examined directly after the addition of a drop of 10% KOH solution and then cultured on selective fungal culture media. The results showed that the rate of infection was more in females, and more in patients with age ranging from (30-39) years old, followed by those between (40-49) years old, and the causative fungi agents of fungal infection of T. pedis were mainly Trichophyton rubrum (72%) followed by Trichophyton mentagrophyte (32%) then Epidermophyton floccosum (12%) respectively. The inhibitory effect of alcoholic extract of Anchusa strigosa was tested in vitro on some causative fungi of Tenia pedis and the result were Compared with that of Antifungal Tolnaftate, and the result of it showed that Trichophyton mentagrophyte was affected by the extract more than Trichophyton rubrum and the percentage of growth inhibition by the extract for Trichophyton mentagrophyte at a concentration of (500) and (1000) P.P.M. were (65.11% and 100%) respectively, while that for Trichophyton rubrum were (53.68%, 87.36% and 100%) at a concentration of (500, 1000 and 1500) P.P.M. respectively. This extract was shown to be a promising antifungal agent in the future. Key words: Anchusa Strigosa, Tinea Pedis, Alcoholic Extract, Dermatophytes, Tolnaftate.
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2

Raversa, Aulia, and Nuria Haristiani. "Can Japanese Speak in Pure Japanese?: The Inevitability of Gairaigo in Japanese." In 3rd International Conference on Language, Literature, Culture, and Education (ICOLLITE 2019). Paris, France: Atlantis Press, 2020. http://dx.doi.org/10.2991/assehr.k.200325.077.

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3

Hosseinpour, Amin, Mahmood A. Mahdavi, and Reza Gheshlaghi. "Role of ohmic resistance on the performance of pure culture microbial fuel cell." In 2012 Iranian Conference on Renewable Energy and Distributed Generation (ICREDG). IEEE, 2012. http://dx.doi.org/10.1109/icredg.2012.6190454.

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4

Fontoura, Inglid, Ricardo Belo, Kumiko Sakane, Maria Angélica Gargione Cardoso, Sônia Khouri, Mituo Uehara, Leandro Raniero, and Airton A. Martin. "FT-IR microspectroscopy in rapid identification of bacteria in pure and mixed culture." In BiOS, edited by Anita Mahadevan-Jansen and Wolfgang Petrich. SPIE, 2010. http://dx.doi.org/10.1117/12.841918.

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Mahmood, Mustafa M., and Ahmed F. Z. Al-Dulaimy. "Effect of media culture, nano and normal micronutrients on some flowers and yield traits of Strawberry." In 1ST SAMARRA INTERNATIONAL CONFERENCE FOR PURE AND APPLIED SCIENCES (SICPS2021): SICPS2021. AIP Publishing, 2022. http://dx.doi.org/10.1063/5.0121341.

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6

Li, Yong-Feng, Nan-Qi Ren, Li-Jie Hu, Guo-Xiang Zheng, and Maryam Zadsar. "Fermentative Biohydrogen Production by Mixed and Pure Bacterial Culture: Designing of Processes and Engineering Control." In ASME 2005 International Solar Energy Conference. ASMEDC, 2005. http://dx.doi.org/10.1115/isec2005-76100.

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In this paper the research about applied bio-hydrogen production engineering is introduced. The advantages, disadvantages and characteristics of bio-hydrogen production systems and some technical issues on anaerobic fermentative bio-hydrogen producing systems will be discussed and focused on the schematic processing, designing strategies and engineering control of fermentation parameters and also the technical means to increase the evolved hydrogen and hydrogen evolution rate. The technology of bio-hydrogen production based on ethanol-type fermentative theory has been established. The mixed continuous culture and pure batch culture processes were proposed for hydrogen production.
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Du, Yongchao, Junfeng Dou, Lirong Cheng, Aizhong Ding, Fuqiang Fan, and Haiying Chen. "Biodegradation of Indeno (1,2,3-cd) Pyrene by a Pure Bacterial Culture of Pandoraea sp." In 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2010. http://dx.doi.org/10.1109/icbbe.2010.5517078.

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BAQER, Batool Abd Al Ameer, and Maysoon Khaleefah ABBAS. "EFFECT OF CEPHALOSPORINS ON ( BIOFILM PRODUCTION AND PROTEASE ) ACTIVITIES BY SOME BACTERIA ISOLATED FROM OTITIS MEDIA." In III.International Scientific Congress of Pure,Appliedand Technological Sciences. Rimar Academy, 2021. http://dx.doi.org/10.47832/minarcongress3-1.

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30 samples (swab) were collected from patients suffering from Otitis media. Swabs were implanted on the culture media blood agar and MacConkey agar to isolate the bacteria and to diagnose them using microscopic, culture and biochemical tests and confirmed by the Vitck-2 system. Of the total, 18 isolates were selected which belong to 8 (26.6%) Staphylococcus aureus, 5 (16.6%) Klebsiella pneumonia, and 4 (13.3%) Escherichia coli. All isolates were investigated for sensitivity to (18) antibiotics, six of them from the cephalosporins group The results showed that all isolates were 100% resistance to Cefotaxime, Ceftazidime, Cefixime, Ceftriaxone, Cefepime, Cefoxitin, Aztronam, Ampenicillin, while the isolates showed the lowest percentage of resistance to Imipenem (4.54) %, while all differences showed a clear difference in some of their resistance. Of the antagonists (Vancomycin, Erythromycin, Rifampin) with a percentage of (95.45, 27.27, 18.18)%, respectively. but for concentrations (4–16)µg/ml were ineffective for some of them. The (Minimal inhibitory concentrations ) MIC test indicated that it ranged between (4–32)µg/ml for Ceftriaxone and (16–32)µg/ml for Ceftazidime. All isolates were shown ability have (100%) activity to produce (Biofilm) was tested on the Congo red agar (CRA) medium, and ability to produce a protease enzyme by (72.22%) on Skim milk agar medium. The results showed a effect of inhibitory concentrations of Ceftriaxone on the activity of biofilm production and the protease enzyme, further the results of this study showed that the following concentrations (1024, 512, 256 and 128)µg/ml were lethal to isolates, while (32–64)µg/ml were inhibitory, Also, the molecular diagnosis shown results of Agarose - gel electrophoresis of both (normal case) S. aureus, E. coli and Kl. pneumonia and heal isolates observed the presence of chromosomal and plasmid DNA bands in the normal status but alone chromosomal DNA bands occur with the isolates deal in Ceftriaxone at levels of (32–128 )µg/ml. In the study of the effect of some Gram-negative bacteria by using IL-2 human. It can be used in the diagnosis of inflammation of the Otitis Media. Key words: Cephalosporins, Otitis media, Biofilm, protease, E. coli, Staphylococcus aureus, Klebsiella pneumonia.
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Gong, Haibo, Antonios Kontsos, Yoontae Kim, Peter I. Lelkes, Qingwei Zhang, Donggang Yao, Kavan Hazeli, and Jack G. Zhou. "Micro Characterization of Mg and Mg Alloy for Biodegradable Orthopedic Implants Application." In ASME 2012 International Manufacturing Science and Engineering Conference collocated with the 40th North American Manufacturing Research Conference and in participation with the International Conference on Tribology Materials and Processing. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/msec2012-7395.

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Magnesium as a candidate metallic biomaterial for biodegradable orthopedic implants was evaluated in-vitro in terms of degradation behavior, biocompatibility and mechanical property both in macro- and micro-scale. Micro structure of pure Mg and AZ61 after degradation in both simulated body fluid (SBF) and cell culture environment were analyzed. Different from AZ61, pure Mg degraded at a higher rate and attracted large amount of salt precipitation which formed a layer covering the surface. Much less pitting degradation and salt deposition were observed on both pure Mg and AZ61 in cell culture environment compared to in SBF. After culturing for 7 days, EAhy926 cells growing on AZ61 showed significant higher proliferation rate as of cells growing on pure Mg. Higher proliferation rates indicated that cells grew better on slow-degrading AZ61 than on fast-degrading pure Mg. Cells growing on AZ61 proliferated much better and assembled together to form a consistent tissue-like micro-structure, while cells spread and reached out on the surface of pure Mg, possibly due to low cell density and lack of cellular communication. The elastic modulus and tensile yield strength of magnesium are closer to those of natural bone than other commonly used metallic biomaterials. It was shown that Mg was biodegradable, biocompatible and had appropriate mechanical strength, thus Mg and its alloys showed great potential for deployment in a new generation of biodegradable orthopedic implants.
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AlBERMANI, Oruba K., Isrra Adnan Auda KHADHIM, and Nebras Mohammed SAHI. "ANTIMICROBIAL SUSCEPTIBILITY PATTERN AND THE ANTAGONISTIC EFFECT OF LACTOBACILLUS IN FIGHTING SHIGELLA SPP. ISOLATED FROM DIARRHEIC CHILDREN." In III.International Scientific Congress of Pure,Appliedand Technological Sciences. Rimar Academy, 2021. http://dx.doi.org/10.47832/minarcongress3-9.

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Background: Shigella is an major source of bacterial gastroenteritis in lack of health awareness in society. The handling of shigellos is mostly requires antibiotics. The using of probiotic of Lactic acid bacteria possess the counter effect against many dangerous bacterial pathogens which associated with gastroenteritis like Shigella spp. Aim: the purpose of this study to estimate the influence of lactic bacteria of the genus Lactobacillus on the population Shigella as a main pathogen involved in gastroenteritis in children. Patients and methods: A total of 50 stool specimens were collected during the period September2019 to January 2020 from diarrheic children patients age range(1-3)years. Standard bacteriological methods were used to isolate, identify, and determine the antimicrobial susceptibility pattern of Shigellaisolates, and we used fresh culture of Lactobacillus 24 h (previously isolated as member of fecal microbiota from healthy person and identified by molecular assay). Then we done centrifugation to obtain supernatant which have test bioactive materials like bacteriocin. These bacteriocin materials subjected to own antibacterial activity against other bacterial pathogen like Shigella spp. By using agar diffusion method . Results: All the 14 Shigella spp. isolates show 100% resistance to nalidixic acid,. cotrimoxazole , and High resistance to ciprofloxacin (85%), and moderate resistant of ampicillin (64%). In agar- well diffusion method indicated the high antagonistic activity of the strains of Lactobacillus 2, 3, and 4 isolated from health GI tract against all Shigella spp., as a result of their activity the total elimination of Shigella within 24 h was observed. conclusion: the Shigella spp. Strains exhibited antibiotic resistant against more one type of antiobiotic The lactobacilii strains tested during this study showed strong antimicrobial activity against Shigella spp. Key words: Shigellos is, Lactobacillus, 16S Rrna.
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Звіти організацій з теми "Pure culture"

1

Fuller, M. E., and J. F. Jr Manning. Differential sensitivity of aerobic gram-positive and gram-negative microorganisms to 2,4,6-trinitrotoluene (TNT) leads to dissimilar growth and TNT transformation: Results of soil and pure culture studies. Office of Scientific and Technical Information (OSTI), July 1996. http://dx.doi.org/10.2172/434457.

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2

Levisohn, Sharon, Maricarmen Garcia, David Yogev, and Stanley Kleven. Targeted Molecular Typing of Pathogenic Avian Mycoplasmas. United States Department of Agriculture, January 2006. http://dx.doi.org/10.32747/2006.7695853.bard.

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Intraspecies identification (DNA "fingerprinting") of pathogenic avian mycoplasmas is a powerful tool for epidemiological studies and monitoring strain identity. However the only widely method available for Mycoplasma gallisepticum (MG) and M. synoviae (MS)wasrandom amplified polymorphic DNA (RAPD). This project aimed to develop alternative and supplementary typing methods that will overcome the major constraints of RAPD, such as the need for isolation of the organism in pure culture and the lack of reproducibility intrinsic in the method. Our strategy focussed on recognition of molecular markers enabling identification of MG and MS vaccine strains and, by extension, pathogenic potential of field isolates. Our first aim was to develop PCR-based systems which will allow amplification of specific targeted genes directly from clinical material. For this purpose we evaluated the degree of intraspecies heterogeneity in genes encoding variable surface antigens uniquely found in MG all of which are putative pathogenicity factors. Phylogenic analysis of targeted sequences of selected genes (pvpA, gapA, mgc2, and lp) was employed to determine the relationship among MG strains.. This method, designated gene targeted sequencing (GTS), was successfully employed to identify strains and to establish epidemiologically-linked strain clusters. Diagnostic PCR tests were designed and validated for each of the target genes, allowing amplification of specific nucleotide sequences from clinical samples. An mgc2-PCR-RFLP test was designed for rapid differential diagnosis of MG vaccine strains in Israel. Addressing other project goals, we used transposon mutagenesis and in vivo and in vitro models for pathogenicity to correlated specific changes in target genes with biological properties that may impact the course of infection. An innovative method for specific detection and typing of MS strains was based on the hemagglutinin-encoding gene vlhA, uniquely found in this species. In parallel, we evaluated the application of amplified fragment length polymorphism (AFLP) in avian mycoplasmas. AFLP is a highly discriminatory method that scans the entire genome using infrequent restriction site PCR. As a first step the method was found to be highly correlated with other DNA typing methods for MG species and strain differentiation. The method is highly reproducible and relatively rapid, although it is necessary to isolate the strain to be tested. Both AFLP and GTS are readily to amenable to computer-assisted analysis of similarity and construction of a data-base resource. The availability of improved and diverse tools will help realize the full potential of molecular typing of avian mycoplasmas as an integral and essential part of mycoplasma control programs.
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3

Bercovier, Herve, and Ronald P. Hedrick. Diagnostic, eco-epidemiology and control of KHV, a new viral pathogen of koi and common carp. United States Department of Agriculture, December 2007. http://dx.doi.org/10.32747/2007.7695593.bard.

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Original objectives and revisions-The proposed research included these original objectives: field validation of diagnostic tests (PCR), the development and evaluation of new sensitive tools (LC-PCR/TaqManPCR, antibody detection by ELISA) including their use to study the ecology and the epidemiology of KHV (virus distribution in the environment and native cyprinids) and the carrier status of fish exposed experimentally or naturally to KHV (sites of virus replication and potential persistence or latency). In the course of the study we completed the genome sequence of KHV and developed a DNA array to study the expression of KHV genes in different conditions. Background to the topics-Mass mortality of koi or common carp has been observed in Israel, USA, Europe and Asia. These outbreaks have reduced exports of koi from Israel and have created fear about production, import, and movements of koi and have raised concerns about potential impacts on native cyprinid populations in the U.S.A. Major conclusions-A suite of new diagnostic tools was developed that included 3 PCR assays for detection of KHV DNA in cell culture and fish tissues and an ELISA assay capable of detecting anti-KHV antibodies in the serum of koi and common carp. The TKPCR assay developed during the grant has become an internationally accepted gold standard for detection of viral DNA. Additionally, the ELISA developed for detecting serum anti-KHV antibodies is now in wide use as a major nonlethal screening tool for evaluating virus status of koi and common carp populations. Real time PCR assays have been able to detect viral DNA in the internal organs of survivors of natural and wild type vaccine exposures at 1 and 10³ genome equivalents at 7 months after exposure. In addition, vaccinated fish were able to transmit the virus to naive fish. Potential control utilizing hybrids of goldfish and common carp for production demonstrated they were considerably more resistant than pure common carp or koi to both KHV (CyHV-3). There was no evidence that goldfish or other tested endemic cyprinids species were susceptible to KHV. The complete genomic sequencing of 3 strains from Japan, the USA, and Israel revealed a 295 kbp genome containing a 22 kbp terminal direct repeat encoding clear gene homologs to other fish herpesviruses in the family Herpesviridae. The genome encodes156 unique protein-coding genes, eight of which are duplicated in the terminal repeat. Four to seven genes are fragmented and the loss of these genes may be associated with the high virulence of the virus. Viral gene expression was studies by a newly developed chip which has allowed verification of transcription of most all hypothetical genes (ORFs) as well as their kinetics. Implications, both scientific and agricultural- The results from this study have immediate application for the control and management of KHV. The proposal provides elements key to disease management with improved diagnostic tools. Studies on the ecology of the virus also provide insights into management of the virus at the farms that farmers will be able to apply immediately to reduce risks of infections. Lastly, critical issues that surround present procedures used to create “resistant fish” must be be resolved (e.g. carriers, risks, etc.). Currently stamping out may be effective in eradicating the disease. The emerging disease caused by KHV continues to spread. With the economic importance of koi and carp and the vast international movements of koi for the hobby, this disease has the potential for even further spread. The results from our studies form a critical component of a comprehensive program to curtail this emerging pathogen at the local, regional and international levels.
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4

Porat, Ron, Doron Holland, and Linda Walling. Identification of Citrus Fruit-Specific and Pathogen-Induced Promoters and Their Use in Molecular Engineering. United States Department of Agriculture, January 2001. http://dx.doi.org/10.32747/2001.7585202.bard.

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This one year BARD project was funded to develop methods to monitor promoter activity a gene expression patterns in citrus fruit. To fulfill this goal, we divided the research tasks between both labs so that the Israeli side evaluated the use of microprojectile bombardment ; a tool to evaluate transient gene expression in various citrus fruit tissues, and the US side optimized technical parameters required for Agrobacterium-mediated transformation of various citrus cultivars. Microprojectile bombardment appeared to be a very efficient method for transient gene expression analysis in citrus leaf tissues but was somewhat less applicable in fruit tissues. Nevertheless, we did succeeded to achieve significant levels of 35S-GUS gene expression in young green flavedo tissue. However, only single random spots of 35S-GUS gene expression were detected mature flavedo and in juice sacs and albedo tissue. Overall, we assume that following some more technical improvements particle bombardment could provide a useful technique to rapidly analyze promoter activity at least in the flavedo tissue. For Agrobacterium-mediated transformation, we found that shoot cultures of 'Washington' navel oranges,'Fairchild' mandarins,'Eureca' lemons,'Troyer' citrange and various grapefruits provided a more reliable and consistent source of tissue for transformation than germinated seedlings. Moreover, various growth media's (McCown, Quoirin & Lepoivre, DCR) further improved shoot and root growth relative to MS mineral media, which is commonly used. Also pure white light (using bulbs which do not emit UV or blue light) improved shoot growth in various citrus varieties, and paromomycin appeared to be a more efficient antibiotic for the selection of transgenic plants than Kanamycin. Overall, these optimizations improve transformation efficacy and shoot growth and rooting capacity. In addition to the development of transformation methods, both Israeli and US labs achieved progress in the identification of citrus fruit-specific promoters. In Israel, we isolated a 3.6 kb promoter fragment of the thiamine biosynthesis c-thi gene, which is highly expressed in fruit peel tissue, whereas in the US we isolated a 1.5 kb promoter fragment of the citrus seed-specific cDNA CssH. The identification of more fruit-specific cDNAs and their corresponding promoter regions is currently in progress.
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