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Добірка наукової літератури з теми "Pseudomonas"

Автор: Grafiati
Опубліковано: 4 червня 2021
Оновлено: 24 серпня 2024

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Статті в журналах з теми "Pseudomonas"

1

Godfrey, S. A. C., S. A. Harrow, J. W. Marshall, and J. D. Klena. "Characterization by 16S rRNA Sequence Analysis of Pseudomonads Causing Blotch Disease of Cultivated Agaricus bisporus." Applied and Environmental Microbiology 67, no. 9 (September 1, 2001): 4316–23. http://dx.doi.org/10.1128/aem.67.9.4316-4323.2001.

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ABSTRACT Bacterial blotch of Agaricus bisporus has typically been identified as being caused by either Pseudomonas tolaasii (brown blotch) or Pseudomonas gingeri(ginger blotch). To address the relatedness of pseudomonads able to induce blotch, a pilot study was initiated in which pseudomonads were selectively isolated from mushroom farms throughout New Zealand. Thirty-three pseudomonad isolates were identified as being capable of causing different degrees of discoloration (separable into nine categories) of A. bisporus tissue in a bioassay. These isolates were also identified as unique using repetitive extragenic palindromic PCR and biochemical analysis. Relationships between these 33 blotch-causing organisms (BCO) and a further 22 selected pseudomonad species were inferred by phylogenetic analyses of near-full-length 16S rRNA gene nucleotide sequences. The 33 BCO isolates were observed to be distributed throughout thePseudomonas fluorescens intrageneric cluster. These results show that in addition to known BCO (P. tolaasii, P. gingeri, and Pseudomonas reactans), a number of diverse pseudomonad species also have the ability to cause blotch diseases with various discolorations. Furthermore, observation of ginger blotch discoloration of A. bisporus being independently caused by many different pseudomonad species impacts on the homogeneity and classification of the previously described P. gingeri.
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2

Freitas, J. Renato de, and James J. Germida. "Pseudomonas cepacia and Pseudomonas putida as winter wheat inoculants for biocontrol of Rhizoctonia solani." Canadian Journal of Microbiology 37, no. 10 (October 1, 1991): 780–84. http://dx.doi.org/10.1139/m91-134.

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Pseudomonas cepacia R55 and R85 and Pseudomonas putida R104, antagonistic towards plant pathogenic fungi in vitro, were assessed as seed inoculants for winter wheat (cv. Norstar) grown in a growth chamber in soil infested with Fusarium solani or Rhizoctonia solani isolate AG-1, AG 2-1, or AG-3. Infestation of soil with R. solani AG-1 or AG 2-1 reduced root dry weight of uninoculated plants by 62 and 78%, respectively, whereas R. solani AG-3 or F. solani had no effect on plant biomass. Pseudomonad inoculants increased (relative to plants subjected to disease) the winter wheat root dry weight by 92–128% and shoot dry weight by 28–48% in the soil infested with R. solani AG-1. The shoot material of all plants inoculated with pseudomonads also had significantly (P < 0.05) higher total Fe contents than the uninoculated treatment in the R. solani AG-1 infested soil. Pseudomonas cepacia R55 produced the highest (P < 0.01) total Fe contents in the shoots, but it had no effect on N and P content. Pseudomonas cepacia R85 significantly increased total N (P < 0.05) and total P (P < 0.01) of wheat shoots, and P. putida R104 increased the percentage (P < 0.05) and (or) total P content (P < 0.01) in the soil infested with R. solani AG-1. Pseudomonas cepacia R85 also significantly (P < 0.05) increased wheat shoot biomass in R. solani AG-3 infested soil. All three pseudomonads produced fluorescent siderophores when cultured in a low-iron medium. These results suggest an in situ antibiosis activity of three fluorescent pseudomonad strains towards phytopathogenic fungi and suggest that the plant growth response was probably due to protection against damage caused by R. solani. Key words: biocontrol, pseudomonads, Rhizoctonia solani, winter wheat, siderophores.
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3

FARRAG, SEHAM A., and ELMER H. MARTH. "Behavior of Listeria monocytogenes when Incubated Together with Pseudomonas Species in Tryptose Broth at 7 and 13°C." Journal of Food Protection 52, no. 8 (August 1, 1989): 536–39. http://dx.doi.org/10.4315/0362-028x-52.8.536.

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Tryptose broth (TB) was inoculated with Listeria monocytogenes (strain Scott A or California), Pseudomonas aeruginosa, Pseudomonas flourescens, or a combination of L. monocytogenes plus Pseudomonas species, and incubated at 7 or 13°C for 8 weeks. McBride Listeria Agar was used to determine numbers of L. monocytogenes and Pseudomonas Isolation Agar to enumerate Pseudomonas species at 0, 7, 14, 28, 42, or 56 d. At 13°C, presence of P. fluorescens had a slight negative effect on growth of L. monocytogenes strain Scott A, and was somewhat detrimental to its survival during the extended incubation. Growth of L. monocytogenes strain California was retarded by presence of P. fluorescens although the maximum population achieved by the pathogen was greater in the presence rather than absence of the pseudomonad; the pseudomonad did have a negative effect on survival of the pathogen. At the same temperature, P. aeruginosa had a negative effect on survival of L. monocytogenes strain California, but had essentially no effect on the other strain of the pathogen. Neither strain of L. monocytogenes affected growth of P. fluorescens nor P. aeruginosa. At 13°C the pH of TB generally decreased when L. monocytogenes grew by itself but increased when either pseudomonad grew by itself or together with the pathogen. At 7°C, growth of both pseudomonads was minimal. Presence of non-growing cells of P. fluorescens retarded somewhat growth of both L. monocytogenes strains early during the incubation. P. aeruginosa had no detectable effect on either strain of L. monocytogenes. The pH of TB decreased when L. monocytogenes grew by itself or together with either pseudomonad, and remained unchanged in TB inoculated with either pseudomonad.
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4

Katsuwon, J., R. Zdor, and A. J. Anderson. "Superoxide dismutase activity in root-colonizing pseudomonads." Canadian Journal of Microbiology 39, no. 4 (April 1, 1993): 420–29. http://dx.doi.org/10.1139/m93-061.

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Several saprophytic fluorescent pseudomonads that are aggressive root colonizers express similar specific activities of superoxide dismutase during growth in liquid culture. The pseudomonads have the potential to produce hydrogen peroxide sensitive and hydrogen peroxide insensitive isoforms of superoxide dismutase with distinct mobilities in nondenaturing polyacrylamide gel electrophoresis. Synthesis of the hydrogen peroxide insensitive form is enhanced by limited iron availability, by exposure to Mn2+, and to a lesser extent by external sources of superoxide anion. Unlike Pseudomonas aeruginosa, a root-colonizing strain of Pseudomonas putida did not show regulation of isoform pattern by phosphate availability. A plasmid potentially encoding the pseudomonad hydrogen peroxide sensitive form complemented the superoxide dismutase deficiency in a mutant of Escherichia coli lacking expression of both Fe and Mn genes. Contact between the plant root and pseudomonad or E. coli cells that lack or express superoxide dismutase did not influence superoxide anion production from root surface enzymes. The pseudomonad and the superoxide dismutase deficient and producing E. coli strains survived exposure to the root equally well. Only the hydrogen peroxide sensitive isoform of superoxide dismutase was detected in P. putida cells associated with bean root surfaces.Key words: pseudomonads, activated oxygen, root surface colonization.
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5

McSpadden Gardener, Brian B. "Diversity and Ecology of Biocontrol Pseudomonas spp. in Agricultural Systems." Phytopathology® 97, no. 2 (February 2007): 221–26. http://dx.doi.org/10.1094/phyto-97-2-0221.

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Diverse Pseudomonas spp. may act as biological controls of plant pathogens, but the ecology of those natural populations is not well understood. And, while biocontrol potential has been identified in multiple pseudomonad strains, the linkages between genotype and phenotype have yet to be fully delineated. However, intensive studies of one class of biocontrol strains, i.e., those that can produce 2,4-diacetylphloroglucionl (DAPG), have provided new insights into the diversity, distribution, and interactions of biocontrol pseudomonads. Those studies also laid the foundation for future research and development of pseudomonad-based biocontrol strategies. Over the past several years, numerous studies have also revealed that biocontrol pseudomonads are widely distributed in agricultural soils, and that multiple crop and soil factors can affect their abundance and activities. Recent work has shown that a variety of farm management practices that reduce soilborne disease pressure can also alter the rhizosphere abundance of DAPG producers in complex ways. Such studies provide support for the hypothesis of an ecological feedback mechanism whereby a native biocontrol population increase and subsequently reduce root disease severity following infection. It is well established that complex biological interactions can take place among bio-control pseudomonads, plant pathogens, their hosts, and other members of the microbial community. The net result of such interactions likely dilutes biocontrol efficacy at the field scale. Nonetheless, inoculation can be effective, and several successful applications of biocontrol pseudomonads have been developed. Future applications of microbial ecology research will hopefully improve the consistency and efficacy of bio-control mediated by Pseudomonas spp. Current applications and future opportunities for improving pseudomonad-based biological control are discussed.
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6

Rahman, Mizanur, Mohammad Jobayer, Nadira Akter, Farook Ahamed, SM Shamsuzzaman, and Kazi Zulfiquer Mamun. "Rapid detection of Pseudomonad at species level by multiplex PCR in surgical units and ICU of Dhaka Medical College Hospital." Bangladesh Journal of Medical Microbiology 10, no. 2 (July 28, 2016): 22–26. http://dx.doi.org/10.3329/bjmm.v10i2.51928.

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Pseudomonads are the most important gram negative organisms involved in various types of infection. This cross sectional study was conducted from January to December 2010 to isolate and identify Pseudomonad at species level in different clinical samples by culture and multiplex polymerase chain reaction (PCR) and to evaluate the efficacy of PCR in rapid detection of the bacteria at species level. Wound swab and tips of endotracheal tube were collected from hospitalized patients from different surgical units and intensive care unit (ICU) of Dhaka Medical College Hospital, Dhaka. Pseudomonads were isolated and identified at species level by culture, microscopy, different biochemical tests and PCR. Among 230 samples, 52.6% were surgical wound, 34.3% were burn wound and 9.6% were traumatic wound samples and 3.4% were tips of endotracheal tubes. Twenty six percent isolated organisms were Pseudomonas spp., 30.4% were Escherichia coli, and 13.5% were Staphylococcus aureus. Others were Proteus, Klebsiella pneumoniae, Acinetobacter baumannii and Enterobacter spp. In 19.67% samples mixed infections by other organism (Esch coli, Staph aureus, Proteus spp, Klebsiella spp) with Pseudomonas were detected and its distribution was highest in traumatic and burn wound. Multiplex PCR and different biochemical tests were used to identify 3 bacterial species of Pseudomonad. Among the species identified, 95.52% was Pseudomonas aeruginosa, 2.99% was Stenotrophomonas maltophilia and 1.49% was Burkholderia cepacia. The sensitivity of multiplex PCR was 95.08% and specificity 94.67%. PCR was the most rapid and more accurate method for detection of Pseudomonad at species level. Bangladesh J Med Microbiol 2016; 10 (2): 22-26
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7

Campbell, James N., Kenneth Conn, Linnea Sorlie, and Fred D. Cook. "Inhibition of growth in canola seedlings caused by an opportunistic Pseudomonas sp. Under laboratory and field conditions." Canadian Journal of Microbiology 32, no. 3 (March 1, 1986): 201–7. http://dx.doi.org/10.1139/m86-041.

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If Pseudomonas rp2, a field-isolated fluorescent pseudomonad, is present on canola (rape) seeds at the time of sprouting, it causes an inhibition of root growth leading to death or delayed maturation of the plant. Inhibitory strains of this type comprise less than 10% of the fluorescent pseudomonads isolated from local field samples, but they were found in widely dispersed sources. By present standards, these Pseudomonas strains would be considered soil saprophytes, since they survive in sterile soil at 4 and −20 °C in the absence of plant material, and since they do not match taxonomically with established plant pathogenic Pseudomonas spp. tested. Under laboratory conditions, inoculation of seeds with Pseudomonas rp2 caused death in 30%, and delayed development in 68% of infected plants. Minimum bacterial load, recoverable from the seed surface and capable of causing inhibition, was 10–20 colony-forming units per seed. The effect of inoculation of seeds with Pseudomonas rp2 on stand, rate of growth, and seed yield was quantitated under field conditions. The results reflected values obtained under laboratory conditions. The potential economic and ecological significance of this type of infection is discussed.
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8

Beaulieu, C., S. Gill, L. Miville, and P. Dion. "Genetic regions of Pseudomonas aureofaciens strain 211 involved in nopaline catabolism." Canadian Journal of Microbiology 34, no. 7 (July 1, 1988): 843–49. http://dx.doi.org/10.1139/m88-145.

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A DNA fragment from the nopaline catabolism region of the Ti plasmid of Agrobacterium tumefaciens showed no detectable homology to the total DNA of several nopaline-utilizing strains of Pseudomonas spp. From one of these pseudomonads, Pseudomonas aureofaciens strain 211, mutants defective in the catabolism of nopaline but not arginine, have been obtained by mutagenesis with transposon Tn5, and also with TnV using a new suicide plasmid vector. The DNA fragment bearing the TnV insertion has been cloned and found to hybridize with DNA of every pseudomonad tested, independently of the capacity to utilize nopaline, but not with Agrobacterium DNA. In a separate experiment, nonmutagenized DNA of strain 211 was cloned in a cosmid vector. Transfer of one of these clones to Pseudomonas putida strain KT2440 conferred on the recipient the capacity for nopaline utilization. However, this cosmid clone was only partially functional, since it did not complement a TnV mutant.
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9

Meyer, Jean-Marie, Valérie A. Geoffroy, Nader Baida, Louis Gardan, Daniel Izard, Philippe Lemanceau, Wafa Achouak, and Norberto J. Palleroni. "Siderophore Typing, a Powerful Tool for the Identification of Fluorescent and Nonfluorescent Pseudomonads." Applied and Environmental Microbiology 68, no. 6 (June 2002): 2745–53. http://dx.doi.org/10.1128/aem.68.6.2745-2753.2002.

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ABSTRACT A total of 301 strains of fluorescent pseudomonads previously characterized by conventional phenotypic and/or genomic taxonomic methods were analyzed through siderotyping, i.e., by the isoelectrophoretic characterization of their main siderophores and pyoverdines and determination of the pyoverdine-mediated iron uptake specificity of the strains. As a general rule, strains within a well-circumscribed taxonomic group, namely the species Pseudomonas brassicacearum, Pseudomonas fuscovaginae, Pseudomonas jessenii, Pseudomonas mandelii, Pseudomonas monteilii, “Pseudomonas mosselii,” “Pseudomonas palleronii,” Pseudomonas rhodesiae, “Pseudomonas salomonii,” Pseudomonas syringae, Pseudomonas thivervalensis, Pseudomonas tolaasii, and Pseudomonas veronii and the genomospecies FP1, FP2, and FP3 produced an identical pyoverdine which, in addition, was characteristic of the group, since it was structurally different from the pyoverdines produced by the other groups. In contrast, 28 strains belonging to the notoriously heterogeneous Pseudomonas fluorescens species were characterized by great heterogeneity at the pyoverdine level. The study of 23 partially characterized phenotypic clusters demonstrated that siderotyping is very useful in suggesting correlations between clusters and well-defined species and in detecting misclassified individual strains, as verified by DNA-DNA hybridization. The usefulness of siderotyping as a determinative tool was extended to the nonfluorescent species Pseudomonas corrugata, Pseudomonas frederiksbergensis, Pseudomonas graminis, and Pseudomonas plecoglossicida, which were seen to have an identical species-specific siderophore system and thus were easily differentiated from one another. Thus, the fast, accurate, and easy-to-perform siderotyping method compares favorably with the usual phenotypic and genomic methods presently necessary for accurate identification of pseudomonads at the species level.
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10

Tryfinopoulou, P., E. Tsakalidou, and G. J. E. Nychas. "Characterization of Pseudomonas spp. Associated with Spoilage of Gilt-Head Sea Bream Stored under Various Conditions." Applied and Environmental Microbiology 68, no. 1 (January 2002): 65–72. http://dx.doi.org/10.1128/aem.68.1.65-72.2002.

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ABSTRACT The population dynamics of pseudomonads in gilt-head sea bream Mediterranean fish (Sparus aurata) stored under different conditions were studied. Phenotypic analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins were performed to identify a total of 106 Pseudomonas strains isolated from S. aurata stored under different temperatures (at 0, 10, and 20°C) and packaging conditions (air and a modified atmosphere of 40% CO2-30% N2-30% O2). Pseudomonas lundensis was the predominant species, followed by Pseudomonas fluorescens, while Pseudomonas fragi and Pseudomonas putida were detected less frequently. Fluorescent Pseudomonas strains dominated under air conditions, while proteolytic and less lipolytic strains dominated under modified-atmosphere packaging. Different storage conditions appear to govern the selection of pseudomonads in gilt-head sea bream fish.
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Більше джерел

Дисертації з теми "Pseudomonas"

1

Frenken, Leo G. "Pseudomonas glumae lipase : characterization, biogenese and protein engineering = Pseudomona glumae lipase /." [S.l. : s.n.], 1993. http://www.gbv.de/dms/bs/toc/131132261.pdf.

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2

Wilson, Neil Lewis. "Integrons in pseudomonads are associated with hotspots of genomic diversity." Phd thesis, Australia : Macquarie University, 2008. http://hdl.handle.net/1959.14/13295.

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Thesis (PhD)--Macquarie University, Division of Environmental & Life Sciences, Department of Biological Sciences, 2008.
Bibliography: p. 257-274.
Literature review -- General materials and methods -- Characterisation of strain collection -- Distribution of integrons and gene cassettes in pseudomonas -- Genomic context of pseudomonas integrons -- Evolutionary analysis of pseudomonas spp. integrons 199 -- Final discussion -- Appendix -- References.
Integrons associated with mobile genetic elements have played a central role in the emergence and spread of multiple antibiotic resistance in many pathogenic bacteria. However, the discovery of integrons in the chromosomes of diverse, non-pathogenic bacteria suggests that integrons have a broader role in bacterial evolution. The Pseudomonas stutzeri species complex is a well studied model for bacterial diversity. Members of the complex are genetically closely related, but sub-taxa are not able to be defined by exclusively shared sets of phenotypic characters. Rather, on the basis of total DNA:DNA similarity, Ps. stutzeri strains have been divided into 17 different groups (termed genomovars). Two Ps. stutzeri strains have been found to contain Chromosomal Integrons (CIs). This thesis involved exploration of the hypothesis that a CI was present in the common ancestor of the Ps. stutzeri species complex and assessed the impact of integrons on diversity across all Pseudomonads. The history and significance of integrons is discussed in Chapter 1 as part of a literature review, and general materials and methods are provided in Chapter 2. Chapters 3 - 6 comprise the sections in which data generated during my PhD project are presented. A comprehensive analysis of the relationships between the strains being analysed is presented in Chapter 3. In Chapter 4, results of PCR and hybridisation screening for integrons across the strain collection are presented. In Chapter 5 the recovery of additional integrons and in depth sequence analysis of the recovered integrons are described. Finally, Chapter 6 contains statistical analyses of integron-associated genes and Chapter 7 contains a final discussion the most significant findings. Twenty-three Pseudomonas spp. strains were screened for the presence of integrons. All but three were found to contain integron-like sequences; however, most integron sequences recovered contained inactivated core integrons. viii Despite having a chromosomal locus, integrons in Pseudomonas were found to have properties indicative of frequent horizontal transfer. Evidence was also obtained which suggests that integrons have been acquired at the same locus on multiple independent occasions. This has not been observed in other families of chromosomal integrons and suggests that the loci at which integrons in Pseudomonas are found are hotspots for recombination.
Mode of access: World Wide Web.
xiii, 274 p. ill
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3

Bredenbruch, Florian. "Einfluss des Pseudomonas-Quinolon-Signals auf die interbakterielle Kommunikation von Pseudomonas aeruginosa." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=979194091.

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4

Manuel, Jerrylynn Laguras. "An Investigation of the Impact of the Stringent Response on the Growth Inhibition of Sclerotinia sclerotiorum by Biocontrol Pseudomonads Pseudomonas sp. DF41 and Pseudomonas chlororaphis PA23." American Society for Microbiology, 2011. http://hdl.handle.net/1993/4791.

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The stringent response (SR) is a global regulatory mechanism that allows bacteria to survive starvation. The plant surface is one environment where a fluctuation in nutrient availability is experienced. Because both Pseudomonas sp. DF41 and Pseudomonas chlororaphis PA23 are able to protect canola from the fungal pathogen Sclerotinia sclerotiorum when applied as a foliar spray, we sought to investigate the impact of this response on the antifungal activities of these two biocontrol strains. The SR exerts its effects on gene transcription through production of the alarmone(p)ppGpp. Metabolism of (p)ppGpp is governed by two enzymes; RelA acts as a synthetase, while SpoT can function as either a synthetase or a hydrolase. To investigate how the SR affects the ability of strains DF41 and PA23 to inhibit the fungal pathogen,relA and relAspoT mutants were generated through allelic exchange. Strain DF41 relA and relAspoT mutants were found to exhibit increased antifungal activity due to enhanced lipopeptide (LP) antibiotic production. Addition of relA, but not spoT in trans restored the mutant phenotype to that of the parent. The influence of the SR on the regulatory mechanisms governing strain DF41 biocontrol was also investigated. It was determined that relA forms part of the Gac regulon while RpoS is under SR control. In fact, addition of rpoS in trans restored protease activity to wild-type levels, but did not attenuate antifungal activity. The SR mutants PA23relA and PA23relAspoT, also exhibited increased growth inhibition of S. sclerotiorum in vitro compared to the wild type. Both mutants showed enhanced production of the antifungal factors pyrrolnitrin, lipase and protease and were complemented by the addition of relA but not spoT. Herein, the SR was found to regulate that Gac system, QS, and RpoS. The presence of gacS or rpoS in multicopy restored the mutant phenotype to that of the wild type.In summary, these findings suggest that the SR negatively influences the biocontrol activities of strains DF41 and PA23. It is evident that the SR is merely one mechanism by which DF41 and PA23-mediated antagonism is regulated.
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5

Turner, Keith Holte. "Bistability in Pseudomonas aeruginosa." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10159.

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The opportunistic pathogen P. aeruginosa is a leading cause of hospital-accquired infections, and is also the primary cause of morbidity and mortality in patients with cystic fibrosis (CF). In this thesis, I describe the identification and characterization of a novel LysR-type transcription regulator (LTTR) of P. aeruginosa named BexR. I show that BexR exhibits reversible ON/OFF bistable expression, which leads to the bistable expression of several genes including one encoding a virulence factor. I present results suggesting that this bistable expression depends on positive feedback of BexR. This work illuminates the simplicity with which a transcription regulatory network can exhibit a complex behavior and generate phenotypic diversity in a clonal population.
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6

Silistre, Hazel. "Riboregulation in Pseudomonas aeruginosa." Thesis, University of Nottingham, 2016. http://eprints.nottingham.ac.uk/32634/.

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The opportunistic human pathogen Pseudomonas aeruginosa controls virulence, production of secondary metabolites, motility, biofilm formation, growth in anaerobic conditions, intracellular and intercellular signalling and the switch from an acute to a chronic mode of infection at the transcriptional and post-transcriptional levels by modulation of the Gac/Rsm system. Cell density-dependent signal accumulation and environmental stimulators such as pH changes and ion limitation activate the GacS/GacA two-component system which in turn triggers transcription of the small regulatory RNAs RsmY and RsmZ. These sRNAs sequester multiple copies of the RNA-binding protein RsmA, antagonising its function. The RsmA/CsrA proteins act as translational repressors by binding to the GGA-motifs in the untranslated region of target mRNAs and blocking ribosome binding. In this study, the biological function of RsmN, an RsmA homologue with a conserved RNA-binding pocket but a distinct protein folding, the predicted autoregulatory mechanism of RsmN, the nature of target transcripts of RsmN, and the cross-regulation between the two Rsm proteins were investigated. The positive control of proteolytic and elastinolytic activities and swarming motility by RsmN has been demonstrated using single and inducible double deletion mutants of rsmN. Furthermore, rsmN deletion increased microcolony formation during biofilm formation. Regulation by RsmN was most apparent in the absence of RsmA, when rsmN expression was induced via a multicopy plasmid and at temperatures lower than 37°C. The double deletion of rsmA and rsmN affected growth, diminished proteolytic and elastinolytic activities, triggered autolysis and led to the increased secretion of the type VI secretion system protein Hcp1. Moreover, the double deletion of rsmA and rsmN altered the colony morphology of P. aeruginosa. Mutagenesis of the functionally critical, conserved RNA-binding residue which is identified as Arg44 in RsmA and Arg62 in RsmN resulted in the loss of RsmN function. In a genome-wide analysis by RNASeq, target transcripts were co-purified with RsmN from 37°C and 34°C cultures of a wild-type strain expressing rsmN in multicopy numbers. RNASeq results indicated that small regulatory RNAs such as CrcZ, RsmY and RgsA are common targets of RsmN and RsmA, whereas PhrS is a target of RsmN only. Other common RsmA and RsmN targets included transcriptional regulators, heat shock proteins, proteases, starvation response proteins, components of the denitrification pathway, outer membrane proteins required for pore formation, type III and type VI secretion system proteins and RsmA. Transcripts of heat shock proteins, the tss operon genes and rsmA were enriched by RsmN at 37°C but not at 34°C whereas the lasB transcript was enriched by RsmN at 34°C but not at 37°C. Based on the list of common targets of RsmA and RsmN and the results obtained from phenotypic assays, induction of the lytic Pf4 prophage, accumulation of alkyl quinolone or c-di-GMP signalling molecules, imbalanced redox state, carbon starvation, increased membrane permeability, and aggregation of misfolded proteins are suggested as possible mechanisms triggering the excessive autolysis of the rsmNind ΔrsmA mutant under uninducing conditions. The data gathered so far suggests that rsmN is differentially expressed, with increased RsmN activity at temperatures below 37°C in comparison with RsmA, and, RsmA and RsmN collectively contribute to the regulation of secondary metabolite production, motility and microcolony formation in P. aeruginosa.
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7

Daly, Philip J. "Permeability of pseudomonas aeruginosa." Thesis, Aston University, 1986. http://publications.aston.ac.uk/12458/.

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8

Ullstrom, Catherine Ann MacDonald. "Comparison of protein OprF from Pseudomonas syringae with protein OprF from Pseudomonas aeruginosa." Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/29200.

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The major outer membrane protein OprF from Pseudomonas aeruginosa was compared with OprF from the fluorescent phytopathogen Pseudomonas syringae. The P. syringae oprF gene was subcloned and sequenced and found to code for a sequence of 344 amino acids containing a 24 amino acid leader sequence. The mature protein, with a deduced molecular weight of 34,225, contained four cysteine residues and an alanine-proline rich area. Comparison of the P. syringae OprF amino acid sequence with the P. aeruginosa OprF and the E. coli OmpA sequences showed that the sequences were most similar at the carboxy-terrninal ends. Restriction enzyme site heterogeneity near the oprF gene from nine different P. syringae pathovars was determined. All pathovars had a conserved SalI site within the gene and conserved PstI. and BamHI sites near the ends of the gene. The location of the PstI and the SalI sites outside the gene was variable, although similar. Immunological relatedness between P. syringae OprF from the different pathovars and P. aeruginosa OprF was confirmed. Protein OprF from all the pathovars was shown to be 2-mercaptoethanol modifiable and more easily heat modifiable than was OprF from P. aeruginosa.
Science, Faculty of
Microbiology and Immunology, Department of
Graduate
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9

Shafik, Hussain latif. "Taxonomie des pseudomonas phytopathogènes du groupe de pseudomonas syringae : études phénotypique et génotypique." Angers, 1994. http://www.theses.fr/1994ANGE0012.

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La taxonomie des pseudomonas fluorescents phytopathogènes oxydase négative bien qu'ayant évolué dans le temps est actuellement floue et insatisfaisante. Ce groupe comprend 52 pathovars de p. Syringae et 5 espèces proches (p. Cichorii et p. Viridiflava, p. Amygdali, p. Ficuserectae, p. Meliae). Les caractères phénotypiques (20 caractères biochimiques et l'assimilation de 147 substrats hydrocarbones) de 655 souches des pathovars de p. Syringae et des 5 espèces proches ont été étudiés. Les données ont été interprétées par l'utilisation d'une méthode de taxonomie numérique et du coefficient de capacite de diagnostic (ccd). Les résultats font apparaitre la grande diversité phénotypique des pathovars p. Syringae. Des souches de certains pathovars comme pv. Glycineae, pv. Phaseolicola, pv. Savastanoi, pv. Porri, pv. Avellanae, pv. Persicae, pv. Tomato, pv. Morsprunorum, pseudomonas sp. (magnolia sp. ) Forment des phenons que l'on différencie par les caractères phénotypiques. D'autres pathovars comme pv. Syringae, pv. Pisi, pv. Aptata, pv. Atropurpurea, pv. Japonica, pv. Dysoxyli, pv. Eriobotryae, pv. Lapsa, pv. Striafaciens et pv. Atrofaciens s'agrègent dans le même phenon a des distances faibles et ne sont pas identifiables par les caractères biochimiques. Les caractères génomiques des souches-type des 52 pathovars de p. Syringae et des 5 espèces proches ont été étudiés par hybridations quantitatives adn/adn par la methode de la nucléase s1. Il a été mis en évidence 9 espèces génomiques qui regroupent un nombre variable de pathovars : p. Syringae (10), p. Savastanoi (20), p. Tomato (14), p. Porri (8), p. Avellanae (2), p. Helianthi (2), p. Tremae (1), p. Cannabinae (1) et p. Viridiflava (2). La validation de ces 9 espèces sera proposée. Cependant, aucune caractérisation biochimique ne permet pas encore leur identification.
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10

Linscott, Andrea J. (Andrea Jane). "Regulatory Divergence of Aspartate Transcarbamoylase from the Pseudomonads." Thesis, University of North Texas, 1996. https://digital.library.unt.edu/ark:/67531/metadc277625/.

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Aspartate transcarbamoylase (ATCase) was purified from 16 selected bacterial species including existing Pseudomonas species and former species reassigned to new genera. An enormous diversity was seen among the 16 enzymes with each class of ATCase being represented. The smallest class, class C, with a catalytically active homotrimer, at 100 kDa, was found in Bacillus and other Gram positive bacteria. In this report, the ATCases from the Gram negatives, Shewanella putrefaciens and Stenotrophomonas maltophilia were added to class C membership. The enteric bacteria typify class B ATCases at 310 kDa, with a dodecameric structure composed of two catalytic trimers coupled to three regulatory dimers. A key feature of class B ATCases is the dissociability of the holoenzyme into regulatory and catalytic subunits which were enzymatically active. In this report, the ATCase from Pseudomonas indigofera was added to class B ATCases. The largest class, at 480 kDa, class A, contains the fluorescent Pseudomonas including most members of the 16S rRNA homology group I. Two polypeptides are produced from overlapping pyrBC' genes. The former, pyrB, encodes a 34 kDa catalytic polypeptide while pyrC' encodes a 45 kDa dihydroorotase-like polypeptide. Two non active trimers are made from six 34 kDa chains which are cemented by six 45 kDa chains to form the active dodecameric structure. Dissociation of the holoenyzme into its separate active subunits has not been possible. In this report, the ATCases from Comamonas acidovorans and C. testosteroni, were added to the class A enzymes. An even larger class of ATCase than class A at 600 kDa was discovered in Burkholderia cepacia. Stoichiometric measurements predict a dodecamer of six 39 kDa polypeptides and six 60 kDa polypeptides. Unlike other large pseudomonads ATCases, the enzyme from B. cepacia was dissociable into smaller active forms. Both the holoenzyme and its dissociated forms were regulated by nucleotide effectors. A new class of ATCase was proposed for B. cepacia type enzymes.
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Книги з теми "Pseudomonas"

1

Ramos, Juan-Luis, ed. Pseudomonas. Boston, MA: Springer US, 2004. http://dx.doi.org/10.1007/978-1-4419-9088-4.

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2

Ramos, Juan-Luis, Johanna B. Goldberg, and Alain Filloux, eds. Pseudomonas. Dordrecht: Springer Netherlands, 2015. http://dx.doi.org/10.1007/978-94-017-9555-5.

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3

Ramos, Juan L., and Alain Filloux, eds. Pseudomonas. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3909-5.

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4

Ramos, Juan-Luis, ed. Pseudomonas. Boston, MA: Springer US, 2004. http://dx.doi.org/10.1007/978-1-4419-9084-6.

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5

Ramos, Juan-Luis, ed. Pseudomonas. Boston, MA: Springer US, 2004. http://dx.doi.org/10.1007/978-1-4419-9086-0.

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6

Montie, Thomas C., ed. Pseudomonas. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4899-0120-0.

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7

Ramos, Juan-Luis, and Alain Filloux, eds. Pseudomonas. Dordrecht: Springer Netherlands, 2007. http://dx.doi.org/10.1007/978-1-4020-6097-7.

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8

Ramos, Juan-Luis, and Roger C. Levesque, eds. Pseudomonas. Boston, MA: Springer US, 2006. http://dx.doi.org/10.1007/0-387-28881-3.

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9

C, Montie Thomas, ed. Pseudomonas. New York: Plenum Press, 1998.

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10

1956-, Ramos Juan-Luis, ed. Pseudomonas. Boston: Kluwer Academic/Plenum, 2004.

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Частини книг з теми "Pseudomonas"

1

Cohen, Taylor S., Dane Parker, and Alice Prince. "Pseudomonas aeruginosa Host Immune Evasion." In Pseudomonas, 3–23. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-9555-5_1.

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2

Pizarro-Tobías, Paloma, Zulema Udaondo, Amalia Roca, and Juan L. Ramos. "Events in Root Colonization by Pseudomonas putida." In Pseudomonas, 251–86. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-9555-5_10.

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3

Holloway, Bruce. "My Memories of Pseudomonas in the Twentieth Century." In Pseudomonas, 289–314. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-9555-5_11.

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4

Dieppois, Guennaelle, Onya Opota, Jorge Lalucat, and Bruno Lemaitre. "Pseudomonas entomophila: A Versatile Bacterium with Entomopathogenic Properties." In Pseudomonas, 25–49. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-9555-5_2.

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5

Hughes, Ami Joy, and Alan R. Hauser. "Pseudomonas Activation of the Inflammasome." In Pseudomonas, 51–74. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-9555-5_3.

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6

Casabona, María-Guillermina, Sylvie Elsen, Valentina Cogoni, and Ina Attrée. "P. aeruginosa Type VI Secretion Machinery: Another Deadly Syringe." In Pseudomonas, 75–97. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-9555-5_4.

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7

Toyofuku, Masanori, George A. O’Toole, and Nobuhiko Nomura. "Anaerobic Life Style of Pseudomonas aeruginosa." In Pseudomonas, 99–117. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-9555-5_5.

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8

Mercado-Blanco, Jesús. "Pseudomonas Strains that Exert Biocontrol of Plant Pathogens." In Pseudomonas, 121–72. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-9555-5_6.

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9

Morita, Yuji, Junko Tomida, and Yoshiaki Kawamura. "Resistance and Response to Anti-Pseudomonas Agents and Biocides." In Pseudomonas, 173–87. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-9555-5_7.

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10

Wysoczynski, Christina L., and Mair E. A. Churchill. "Lessons from the QSCR Structure for Quorum Sensing." In Pseudomonas, 189–214. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-017-9555-5_8.

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Тези доповідей конференцій з теми "Pseudomonas"

1

Нейдорф, А. Р., М. А. Морозова, and М. М. Оганисян. "THE PROBLEM OF ASSESSING WATER QUALITY IN THE CULTIVATION OF STURGEON IN THE USV." In DEVELOPMENT AND MODERN PROBLEMS OF AQUACULTURE. ООО "ДГТУ-Принт" Адресс полиграфического предприятия 344003 пл Гагарина, зд. 1, 2023. http://dx.doi.org/10.23947/aquaculture.2023.79-81.

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The article discusses the results of the analysis of microbiological indicators of water quality in the basins of the UZV. The analysis of pool water was carried out according to 8 indicators (HPV, E. coli, generalized coliform bacteria, spores of sulfite-reducing clostridium, enterococci, Pseudomonas aeromonas, Pseudomonas). Pathogenicity and antibiotic resistance factors of isolated strains of pathogenic aeromonads and pseudomonads were determined
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2

Vlasova, A. I., and O. M. Minaeva. "Efficiency of Pseudomonas aureofaciens colonization in wheat rhizosphere under model condition." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.274.

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The survival of Pseudomonas aureofaciens in the wheat rhizosphere was studied. A significant increase in the number of pseudomonads and tetracycline resistant bacteria was shown when introduced into the model system of strains.
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3

Gerasimchuk, A. L., D. V. Antsiferov, P. A. Bukhtiyarova, and D. A. Ivasenko. "OBTAINING A LIPOPHILIC BACTERIAL CONSORTIUM FOR FAT DEPOSITS UTILIZATION." In STATE AND DEVELOPMENT PROSPECTS OF AGRIBUSINESS. DSTU-PRINT, 2020. http://dx.doi.org/10.23947/interagro.2020.1.81-84.

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Bacterial strains with lipolytic activities Pseudomonas citronellolis A13, Pseudomonas extremaustralis Б11, Pseudomonas nitroreducens Д14, Pseudomonas sp. B21, Microvirgula curvata B37, Brevibacillus sp. 28Д, Bacillus subtilis spp. B10, B34 were isolated from fat-containing industrial wastewater of food industry and were used for obtaining a lipophilic bacterial consortium that perspective for fat deposits utilization.
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4

Kokoulin, M. S., E. V. Sokolova, L. A. Romanenko, Yu N. Elkin та N. A. Komandrova. "Липополисахариды морских бактерий рода Pseudomonas". У XVI Всероссийская молодежная школа-конференция памяти В. Е. Васьковского "Актуальные проблемы химии и биологии". Дальневосточное отделение Российской академии наук, 2017. http://dx.doi.org/10.47471/16_2017_09_04_09_09.

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5

Smirnova, S. V., and А. A. Zhdanova. "VIRULENCE FACTORS OF PSEUDOMONAS AERUGINOSA." In Инновационное развитие агропромышленного, химического, лесного комплексов и рациональное природопользование, 218–23. НовГУ им. Ярослава Мудрого, 2023. http://dx.doi.org/10.34680/978-5-89896-873-1/2023.innovation.38.

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6

Zeng, Xiaoxi, Xueduan Liu, Pei Jiang, Wen Li, and Jianxin Tang. "Cadmium Removal by Pseudomonas aeruginosa E1." In 2009 International Conference on Energy and Environment Technology. IEEE, 2009. http://dx.doi.org/10.1109/iceet.2009.351.

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7

Mallappa, Anupama, Catriona Middleton, Stewart Cox, Aditi Majethia, and Lynne Mcdonald. "1186 Pseudomonas in the ‘hot tub’." In Royal College of Paediatrics and Child Health, Abstracts of the RCPCH Conference–Online, 15 June 2021–17 June 2021. BMJ Publishing Group Ltd and Royal College of Paediatrics and Child Health, 2021. http://dx.doi.org/10.1136/archdischild-2021-rcpch.458.

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8

Pilipchuk, T. A., L. N. Valentovich, and E. I. Kalamiyets. "Genome characterization of Pseudomonas phages BIM BV-45 and BIM BV-46 – the components of biopesticide Multiphage to control bacterial diseases of vegetable crops." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.197.

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The conducted investigation resulted in full nucleotide sequencing of Pseudomonas phage BIM BV-45 and Pseudomonas phage BIM BV-46 (registration numbers in GenBank NCBI MT094430 and MT094431) – components of biopesticide Multiphage.
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9

Boynton, Lin, Ilenne Del Valle, Ilenne Del Valle, Hsiao-Ying Cheng, Hsiao-Ying Cheng, Carrie A. Masiello, Carrie A. Masiello, Joff Silberg, and Joff Silberg. "ENGINEERING PSEUDOMONAS STUTZERI AS A BIOGEOCHEMICAL BIOSENSOR." In GSA Annual Meeting in Denver, Colorado, USA - 2016. Geological Society of America, 2016. http://dx.doi.org/10.1130/abs/2016am-286788.

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10

Alzubaidy, Mohanad W. Mahdi, Asmaa M. Salih Almohaidi, Ammar Ahmed Sultan, and Sana M. H. AL-Shimmary. "Virulence gene of Pseudomonas aeruginosa with nanoparticle." In XIAMEN-CUSTIPEN WORKSHOP ON THE EQUATION OF STATE OF DENSE NEUTRON-RICH MATTER IN THE ERA OF GRAVITATIONAL WAVE ASTRONOMY. AIP Publishing, 2019. http://dx.doi.org/10.1063/1.5116966.

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Звіти організацій з теми "Pseudomonas"

1

Ornston, L. N. Negative Control of Biodegradation in Pseudomonas. Fort Belvoir, VA: Defense Technical Information Center, March 1988. http://dx.doi.org/10.21236/ada193875.

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2

Krawiec, S. Cloning and expression of the sulfoxide/sulfone/sulfonate/sulfate genes in Pseudomonads and Thiobacillae. [Pseudomonas, Thiobacillus, Rhodococcus]. Office of Scientific and Technical Information (OSTI), February 1992. http://dx.doi.org/10.2172/5653703.

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3

Lewis, Kim. Genetics of Persister Formation in Pseudomonas aeruginosa. Fort Belvoir, VA: Defense Technical Information Center, December 2012. http://dx.doi.org/10.21236/ada580295.

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4

Schnider, Shirley. The biological properties of Pseudomonas aeruginosa bacteriophage 7V. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.771.

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5

Lessie, T. G. Genomic plasticity and catabolic potential of Pseudomonas cepacia. Office of Scientific and Technical Information (OSTI), April 1996. http://dx.doi.org/10.2172/224251.

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6

Blake, Robert, and III. Transformation and Precipitation of Toxic Metals by 'Pseudomonas maltophilia'. Fort Belvoir, VA: Defense Technical Information Center, May 1991. http://dx.doi.org/10.21236/ada238232.

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7

Blake, Robert, and II. Transformation and Precipitation of Toxic Metals by Pseudomonas maltophilia. Fort Belvoir, VA: Defense Technical Information Center, May 1990. http://dx.doi.org/10.21236/ada224329.

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8

Daniel P. Molloy. Factors Affecting Zebra Mussel Kill by the Bacterium Pseudomonas fluorescens. Office of Scientific and Technical Information (OSTI), February 2004. http://dx.doi.org/10.2172/876491.

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9

Frost, John W. Green Synthesis of Phloroglucinol: Exploiting Pseudomonas fluorescens and Scale-Up. Fort Belvoir, VA: Defense Technical Information Center, January 2008. http://dx.doi.org/10.21236/ada593488.

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10

Benson, Deanne. A study of RNA bacteriophage 7s infection of Pseudomonas aeruginosa. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.2139.

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