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Добірка наукової літератури з теми "Primary cell culture of bivalve"

Автор: Grafiati
Опубліковано: 8 червня 2024

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Статті в журналах з теми "Primary cell culture of bivalve":

1

Yoshino, T. P., U. Bickham, and C. J. Bayne. "Molluscan cells in culture: primary cell cultures and cell lines." Canadian Journal of Zoology 91, no. 6 (June 2013): 391–404. http://dx.doi.org/10.1139/cjz-2012-0258.

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In vitro cell culture systems from molluscs have significantly contributed to our basic understanding of complex physiological processes occurring within or between tissue-specific cells, yielding information unattainable using intact animal models. In vitro cultures of neuronal cells from gastropods show how simplified cell models can inform our understanding of complex networks in intact organisms. Primary cell cultures from marine and freshwater bivalve and gastropod species are used as biomonitors for environmental contaminants, as models for gene transfer technologies, and for studies of innate immunity and neoplastic disease. Despite efforts to isolate proliferative cell lines from molluscs, the snail Biomphalaria glabrata (Say, 1818) embryonic (Bge) cell line is the only existing cell line originating from any molluscan species. Taking an organ systems approach, this review summarizes efforts to establish molluscan cell cultures and describes the varied applications of primary cell cultures in research. Because of the unique status of the Bge cell line, an account is presented of the establishment of this cell line, and of how these cells have contributed to our understanding of snail host – parasite interactions. Finally, we detail the difficulties commonly encountered in efforts to establish cell lines from molluscs and discuss how these difficulties might be overcome.
2

Potts, Robert W. A., Alejandro P. Gutierrez, Yennifer Cortés-Araya, Ross D. Houston, and Tim P. Bean. "Developments in marine invertebrate primary culture reveal novel cell morphologies in the model bivalve Crassostrea gigas." PeerJ 8 (June 1, 2020): e9180. http://dx.doi.org/10.7717/peerj.9180.

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Cell culture provides useful model systems used in a wide range of biological applications, but its utility in marine invertebrates is limited due to the lack of immortalised cell lines. Primary cell and tissue cultures are typically used but remain poorly characterised for oysters, which can cause issues with experimental consistency and reproducibility. Improvements to methods of repeatable isolation, culture, and characterisation of oyster cells and tissues are required to help address these issues. In the current study, systematic improvements have been developed to facilitate the culture of primary cells from adult Pacific oyster tissues and identify novel cell morphologies that have not been reported previously. Cultures analysed by light microscopy, qPCR, and live cell imaging demonstrated maintenance of live, metabolically active Pacific oyster cells for several weeks post-explant. Interestingly, whole hearts dissected from adult oysters were found to continue contracting rhythmically up to 8 weeks after being transferred to a tissue culture system. Mantle tissue explants were also actively moving in the culture system. These improvements in primary cell culture of bivalves may be beneficial for research in ecotoxicology, virology, immunology, and genetic resistance to disease.
3

Dessai, Shanti Nilesh. "Primary culture of mantle cells of bivalve mollusc, Paphia malabarica." In Vitro Cellular & Developmental Biology - Animal 48, no. 8 (August 8, 2012): 473–77. http://dx.doi.org/10.1007/s11626-012-9538-4.

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4

Odintsova, N. A., and A. V. Khomenko. "Primary cell culture from embryos of the Japanese scallop Mizuchopecten yessoensis (Bivalvia)." Cytotechnology 6, no. 1 (May 1991): 49–54. http://dx.doi.org/10.1007/bf00353702.

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5

Morgan, Siân R., Laura Paletto, Benjamin Rumney, Farhana T. Malik, Nick White, Philip N. Lewis, Andrew R. Parker, Simon Holden, Keith M. Meek, and Julie Albon. "Establishment of long-term ostracod epidermal culture." In Vitro Cellular & Developmental Biology - Animal 56, no. 9 (October 2020): 760–72. http://dx.doi.org/10.1007/s11626-020-00508-8.

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Abstract Primary crustacean cell culture was introduced in the 1960s, but to date limited cell lines have been established. Skogsbergia lerneri is a myodocopid ostracod, which has a body enclosed within a thin, durable, transparent bivalved carapace, through which the eye can see. The epidermal layer lines the inner surface of the carapace and is responsible for carapace synthesis. The purpose of the present study was to develop an in vitro epidermal tissue and cell culture method for S. lerneri. First, an optimal environment for the viability of this epidermal tissue was ascertained, while maintaining its cell proliferative capacity. Next, a microdissection technique to remove the epidermal layer for explant culture was established and finally, a cell dissociation method for epidermal cell culture was determined. Maintenance of sterility, cell viability and proliferation were key throughout these processes. This novel approach for viable S. lerneri epidermal tissue and cell culture augments our understanding of crustacean cell biology and the complex biosynthesis of the ostracod carapace. In addition, these techniques have great potential in the fields of biomaterial manufacture, the military and fisheries, for example, in vitro toxicity testing.
6

Odintsova, Nelly A., Vyacheslav A. Dyachuk, and Leonid P. Nezlin. "Muscle and neuronal differentiation in primary cell culture of larval Mytilus trossulus (Mollusca: Bivalvia)." Cell and Tissue Research 339, no. 3 (February 6, 2010): 625–37. http://dx.doi.org/10.1007/s00441-009-0918-3.

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7

Le Marrec-Croq, F., D. Glaise, C. Guguen-Guillouzo, C. Chesne, A. Guillouzo, V. Boulo, and G. Dorange. "Primary cultures of heart cells from the scallp Pecten maximus (mollusca-bivalvia)." In Vitro Cellular & Developmental Biology - Animal 35, no. 5 (May 1999): 289–95. http://dx.doi.org/10.1007/s11626-999-0073-x.

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8

Сhadaeva, А. А., O. S. Povolyaeva, and S. G. Yurkov. "Primary cell culture in virology." "Veterinary Medicine" Journal 23, no. 01 (January 2020): 51–54. http://dx.doi.org/10.30896/0042-4846.2020.23.1.51-54.

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9

Ren, Daan, and Joseph D. Miller. "Primary cell culture of suprachiasmatic nucleus." Brain Research Bulletin 61, no. 5 (September 2003): 547–53. http://dx.doi.org/10.1016/s0361-9230(03)00193-x.

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10

Dyachuk, Vyacheslav. "Extracellular matrix is required for muscle differentiation in primary cell cultures of larval Mytilus trossulus (Mollusca: Bivalvia)." Cytotechnology 65, no. 5 (May 9, 2013): 725–35. http://dx.doi.org/10.1007/s10616-013-9577-z.

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Статті в журналах Дисертації Книги

Дисертації з теми "Primary cell culture of bivalve":

1

Fleurbaix, Emmanuel. "Évaluation écotoxicologique des éléments terres-rares : approches cellulaires chez différentes espèces aquatiques." Electronic Thesis or Diss., Université de Lorraine, 2021. http://www.theses.fr/2021LORR0324.

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Depuis 30 ans, l’utilisation croissante des Lanthanides dans les nouvelles technologies a entraîné des rejets importants de ces métaux vers les écosystèmes aquatiques. Dans une politique mondiale de développement durable visant à préserver la qualité des écosystèmes, la question de l’impact des Lanthanides sur les organismes aquatiques s’est naturellement posée. Néanmoins, les études restent peu nombreuses et aucun consensus ne subsiste concernant la toxicité des Lanthanides. Dans ce contexte, nous avons étudié la toxicité cellulaire des Lanthanides individuellement et en mélanges. Les effets toxiques ont été mis en évidence en mesurant la viabilité de cellules fibroblastiques (ZF4 ; ATCC®, CRL-2050™) et hépatiques (ZFL ; ATCC®, CRL-2643™) de poisson zèbre (Danio rerio), de cellules branchiales (RTgill-W1 ; ATCC®, CRL-2523™) de la truite arc-en-ciel (Oncorynchus mykiss), et des cellules primaires de glandes digestives de corbicule (Corbicula fluminea) exposées à ces métaux. Les résultats ont montré que les Lanthanides sont responsables d’effets toxiques directs sur nos modèles cellulaires. Concernant la toxicité des Lanthanides en mélanges, des effets synergiques ont été observés sur les 3 lignées cellulaires de poissons. Nous nous sommes également intéressés aux mécanismes de détoxification des Lanthanides dans les cellules ZF4 de Danio rerio. Nous avons décidé d’étudier ces acteurs en raison de leurs rôles respectifs dans les phases II et III de la détoxification cellulaire de métaux chez les bivalves et les poissons. Pour cela, la viabilité des cellules ZF4 a été mesurée après des expositions aux Lanthanides en présence d’inhibiteurs spécifiques des glutathion-S-transférases (acide éthacrynique) et des protéines MRP (MK571 et probénécide). Les résultats ont montré que les protéines MRP sensibles au MK571 jouent un rôle dans la détoxification des Lanthanides dans les cellules ZF4. Globalement, les résultats obtenus pour cette recherche ont confirmé que les effets toxiques des Lanthanides à l’échelle cellulaire sont pertinents pour prédire les effets in vivo, dans le cadre d’une évaluation de la toxicité de ces métaux
Since 30 years ago, the growing use of Lanthanides in new technologies has contributed to important releases of these metals into aquatic ecosystems. In a global sustainable development policy aimed at preserving the quality of ecosystems, the impact of Lanthanides on aquatic organisms has naturally been questioned. However, studies on the aquatic ecotoxicology of Lanthanides are incomplete, and no consensus is established yet. In this context, we studied the cellular toxicity of Lanthanides individually and in mixtures. To determine these toxic effects, cell viability was measured on Danio rerio fibroblast-like cells (ZF4; ATCC®, CRL-2050™), Danio rerio hepatic cells (ZFL; ATCC®, CRL-2643™), Oncorhynchus mykiss epithelial cells (RTgill-W1; ATCC®, CRL-2523™), and primary culture of Corbicula fluminea digestive glands exposed to Lanthanides. Direct toxicity of Lanthanides has been observed on all cellular models. Concerning the toxicity of Lanthanides in mixtures, synergistic effects have been underlined on the three fish cell lines. In this research, we focused on the mechanisms of the detoxification of Lanthanides in the case of ZF4 cells from Danio rerio. The effects of Lanthanides were assessed in the presence of specific inhibitors of glutathione-S-transferases (ethacrynic acid) and MRP-like (MK571 and probenecid), by cell viability measurements. We decided to study these actors of the cellular detoxification due to their respective roles in phases II and III of the cellular detoxification of metals in fishes and bivalves. Regarding the results, MRP-like proteins are effectively involved in the detoxification of Lanthanides in ZF4 cells. Overall, our results highlighted the relevance of the toxic effects of Lanthanides at the cellular level for the risk assessment of these metals
2

Birmelin, Claudia. "Development of primary cell culture systems from marine invertebrates for use in toxicology." Thesis, University of Surrey, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.265684.

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3

Bohm, Maren [Verfasser]. "The role of sialic acids in avian influenza virus infection of primary cell culture / Maren Bohm." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2010. http://d-nb.info/1004206291/34.

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4

Tradewell, Miranda Lee. "The central role of calcium dysregulation in a primary cell culture model of amyotrophic lateral sclerosis." Thesis, McGill University, 2009. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=32355.

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Amyotrophic lateral sclerosis (ALS; aka Lou Gehrig Disease) is an adult-onset, rapidly progressing motor neuron disease for which there is currently very little treatment. Glutamate excitoxicity, formation of proteinaceous inclusions, proteasome impairment, and mitochondrial dysfunction have been associated with both sporadic and familial ALS, but there are many questions about how these events fit together to cause motor neuron dysfunction and death. In culture models of familial ALS due to mutations in the enzyme Cu/Zn-superoxide dismutase (SOD1), treatments that reduce intracellular Ca2+ prolong viability and prevent formation of mutant SOD1 inclusions. Motor neurons are vulnerable to Ca2+ overload due to poor Ca2+ buffering and high glutamatergic input. Thus, disruption of calcium homeostasis may play an early and key role in ALS. The goal of this thesis was to investigate how levels of Ca2+ in cytosol and calcium-buffering organelles (mitochondria and endoplasmic reticulum) change in motor neurons in an experimental model of ALS and how these changes relate to other hallmarks of ALS pathogenesis (impaired mitochondrial and proteasome function). To achieve this goal, ALS-causing G93A-SOD1 was expressed in motor neurons of dissociated murine spinal cord-dorsal root ganglia cultures. Using microfluorometric techniques, increases in mitochondrial Ca2+ ([Ca2+]m) and endoplasmic reticular Ca2+ ([Ca2+]ER) were observed prior to an increase in cytosolic Ca2+ ([Ca2+]c). Decrease in mitochondrial membrane potential and rounding of their shape was observed concomitant with the early increase in [Ca2+]m. A further increase in [Ca2+]c was observed in motor neurons with mutant
La sclérose latérale amyotrophique (SLA, alias la maladie de Lou Gehrig) est une maladie neuromusculaire à évolution rapide qui commence à l'âge adulte, et pour laquelle il existe présentement très peu de traitements. L'excitotoxicité du glutamate, la formation d'inclusions protéiques, la déficience du protéasome et le dysfonctionnement mitochondrial ont tous été associés à la SLA sporadique et héréditaire, mais on se questionne toujours sur ce qui rassemble ces éléments qui, ensemble, mènent au dysfonctionnement neuromusculaire et au décès. Dans les modèles de culture de SLA héréditaire causée par des mutations de l'enzyme Cu/Zn-superoxyde dismutase (SOD1), les traitements réduisant le Ca2+ intra-cellulaire prolongent la viabilité et empêchent la formation d'inclusions mutantes de la SOD1. Les motoneurones sont vulnérables aux surcharges de Ca2+ causées par une régulation du Ca2+ inadéquate et par un grand apport glutamatergique. Ainsi, la perturbation de l'homéostasie du calcium joue peut-être un rôle précoce important dans la SLA. Le but de cette thèse était d'enquêter sur la façon dont les niveaux de Ca2+ à l'intérieur des organites assurant la régulation du cytosol et du calcium (mitochondrie et réticulum endoplasmique) changent dans les motoneurones d'un modèle expérimental de la SLA, ainsi que sur la façon dont ces changements sont liés à d'autres marques de pathogénie de la SLA (détérioration du fonctionnement mitochondrial et du protéasome). Afin d'atteindre cet objectif, la G93A-SOD1 causant la SLA a été introduite dans les cultures de motoneurones provenant de neurones ganglions de la racine dorsale mu
5

Stab, II Bernd Robert. "The Effects of Cell Culture Oxygen Levels on the Replicative Senescence Processes of Primary Human Fibroblasts." Diss., Virginia Tech, 2009. http://hdl.handle.net/10919/28468.

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Serial passaging of primary human fibroblasts leads to the formation of non-dividing senescent cells by a process termed replicative senescence. This tissue culture-based methodology is currently used as a model system to determine the underlying mechanisms of in vivo cellular aging and tumor suppression. Senescence is regarded as an alternative pathway to apoptosis, where cells undergo multiple changes in metabolic and cellular signaling pathways in order to prevent proliferation but still maintain a metabolically-active cell. Whether or not this model accurately reflects in vivo processes is presently controversial; however, replicative senescence is currently the most applicable model through which one can investigate the underlying causes of human cellular aging in the context of controlled environmental stress over time. This work was directed at understanding the molecular processes involved in replicative senescence with specific emphasis on the role of the mitochondria. A series of experiments were performed to assess changes during the induction of replicative senescence under conditions of low (3%) and high (20%) oxygen levels. Measurements were made at the transcriptional, protein, and metabolite levels. Microscopy wasalso utilized to monitor changes in mitochondrial morphology and volume. While previous studies have evaluated specific pathways and/or products; this work combines a more complete metabolomic, genomic, proteomic, and morphological picture of cells undergoing senescence and oxidative stress. Considering the low cell population densities of primary adherent fibroblasts and the subsequent low concentrations of small polar metabolites involved in glycolysis and the TCA cycle, methodologies needed to be developed in order to optimize metabolite extraction and liquid chromatography-mass spectrometric analysis. Protein kinase and transcriptional microarrays were also performed in order to quantify the changes in activated/deactivated signaling cascades as well as gene expression and relate these findings to metabolomic data. Mitochondrial dynamics of cells at different age time points and under different oxygen conditions were also assessed including mitochondrial size, shape, membrane potential, and percent volume per cell volume using confocal microscopy. The results obtained not only confirm the major pathways involved in senescence (p53/p21, PTEN/p27, and RTK/Raf/MAPK) but also provide evidence at both the transcriptional and protein levels for additional senescence-associated pathways. The majority of the changes observed were related to pathways involved in cellular stress, cell cycle control, and the survival response. Metabolic data suggested a –pooling effect– of glycolysis and TCA precursor molecules due to attenuation in enzyme function; this theory was also supported by an observed up regulation of gene expression as a compensatory mechanism. Mitochondria exhibited changes in membrane potential as well as volume and percent volume per cell which suggested compensatory hypertrophy and/or attenuation of mitochondrial fission processes. When the aforementioned analyses are tied together, a “theoretical model of senescence” can be formulated and is characterized by increased metabolic protein and associated metabolite levels due to attenuation in their respective enzyme function, resulting in increases in expression of their associated genes as a compensatory mechanism.
Ph. D.
6

Hang, Ta-Chun. "Optimization of primary endothelial culture methods and assessment of cell signaling pathways in the context of inflammation." Thesis, Massachusetts Institute of Technology, 2012. http://hdl.handle.net/1721.1/71467.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biological Engineering, 2012.
Cataloged from PDF version of thesis.
Includes bibliographical references.
Tissue engineering is a potentially valuable tool for clinical treatment of diseases where host tissues or organs need to be replaced. Progression of engineering metabolically complex organs and tissues has been severely limited by the lack of established, functional vasculature. The thesis work described herein focused on methods of establishing and studying specific endothelial cell types in vitro for potential applications in establishing functional microvascular architecture. To achieve these objectives, a model system of primary liver sinusoidal endothelial cells (LSEC) was initially studied due to the high metabolic requirements of the liver, as well as the unique phenotype that they possess. We were able to demonstrate that free fatty acids were able to rescue LSEC in culture, promote proliferation, and maintain their differentiated phenotype. Our work with lipid supplementation in serum-free conditions provides flexibility in engineering liver tissue with a functional vasculature comprised with relevant endothelial types encountered in vivo. Following up our work with LSEC, we explored the human dermal microvascular endothelial cell (HDMVEC) system to understand the signaling mechanisms involved in sprouting angiogenesis. Engineered tissues that are implanted will require integration with host vasculature. We established a method to collect large signaling data sets from a physiologically relevant in vitro culture system of HDMVEC that permitted angiogenic sprouting. We were able to find statistically significant data regarding how angiostatic cues like Platelet Factor 4 can modulate angiogenesis signaling pathways. Our results from working with both types of endothelial cell systems provide insight into potential methods for establishing specialized microvasculature for engineered tissues, both in propagation of differentiated endothelial cells in vitro and promotion of tissue/organ survival following their implantation.
by Ta-Chun Hang.
Ph.D.
7

Kraft, Robert, Allon Kahn, José L. Medina-Franco, Mikayla L. Orlowski, Cayla Baynes, Fabian López-Vallejo, Kobus Barnard, Gerald M. Maggiora, and Linda L. Restifo. "A cell-based fascin bioassay identifies compounds with potential anti-metastasis or cognition-enhancing functions." The Company of Biologists, 2013. http://hdl.handle.net/10150/605272.

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A first-of-its-kind, proof-of-concept drug screen with implications for two unmet medical needs.
The actin-bundling protein fascin is a key mediator of tumor invasion and metastasis and its activity drives filopodia formation, cell-shape changes and cell migration. Small-molecule inhibitors of fascin block tumor metastasis in animal models. Conversely, fascin deficiency might underlie the pathogenesis of some developmental brain disorders. To identify fascin-pathway modulators we devised a cell-based assay for fascin function and used it in a bidirectional drug screen. The screen utilized cultured fascin-deficient mutant Drosophila neurons, whose neurite arbors manifest the 'filagree' phenotype. Taking a repurposing approach, we screened a library of 1040 known compounds, many of them FDA-approved drugs, for filagree modifiers. Based on scaffold distribution, molecular-fingerprint similarities, and chemical-space distribution, this library has high structural diversity, supporting its utility as a screening tool. We identified 34 fascin-pathway blockers (with potential anti-metastasis activity) and 48 fascin-pathway enhancers (with potential cognitive-enhancer activity). The structural diversity of the active compounds suggests multiple molecular targets. Comparisons of active and inactive compounds provided preliminary structure-activity relationship information. The screen also revealed diverse neurotoxic effects of other drugs, notably the 'beads-on-a-string' defect, which is induced solely by statins. Statin-induced neurotoxicity is enhanced by fascin deficiency. In summary, we provide evidence that primary neuron culture using a genetic model organism can be valuable for early-stage drug discovery and developmental neurotoxicity testing. Furthermore, we propose that, given an appropriate assay for target-pathway function, bidirectional screening for brain-development disorders and invasive cancers represents an efficient, multipurpose strategy for drug discovery.
8

Song, Miyeoun. "Organ and primary culture of medaka (Oryzias latipes) testis: Test systems for the analysis of cell proliferation and differentiation." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2003. http://nbn-resolving.de/urn:nbn:de:swb:14-1059039500031-89370.

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In cultured medaka testis fragments, cells remained viable for the entire culture period (17h), and spermatids that developed from spermatocytes were viable and motile. Primary cultures were characterized over a period of two days with respect to cell viability and the distribution of adherent and suspended cells. These two cell populations were maintained in dynamic equilibrium in vitro for several days. Proliferating cells were predominant among clusters of suspended cells, as determined by BrdU labeling, and CFSE and propidium iodide PI labeling. Based on cytological criteria, the proliferating cells were mostly spermatogonia and possibly also preleptotene spermatocytes. Differentiation of spermatocytes into spermatids or spermatozoa was also observed, mainly among the suspended cells. These results suggest that the organ and primary culture systems are suitable systems for studying the effects of substances that interfere with spermatogenesis in the medaka, a model vertebrate. The organ and primary culture systems were used to analyze the effects of a synthetic estrogen, EE2, on cell proliferation in medaka testis. Both organ and primary culture were suitable for this purpose consistently small concentrations (0.01 and 1 nM) of EE2 stimulated cell proliferation slightly, while higher concentrations (100 nM) had an inhibitory effect. To investigate the effect of phytoestrogens on cell proliferation in spermatogenesis, selected flavonoids [genistein (1, 10, 100 µg/ml), quercetin (0.01, 1, 100 µM), and 8-prenylnarigenin (0.001, 0.1, 1, 10 µM)] were added to medaka testis primary cultures. Genistein and quercetin inhibited cell proliferation in the cultures while 8-prenylnarigenin had no effect. In a second series of experiments the addition of genistein (10 µg/ml) to primary cultures significantly inhibited both cell proliferation and cell differentiation as determined by flow cytometry using CFSE/PI labeling.
9

Klingbeil, Maria Fátima Guarizo. ""Comparação de dois métodos de obtenção celular para cultura primária de queratinócitos bucais humanos"." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/85/85131/tde-17052007-144619/.

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Freqüentemente as condutas terapêuticas utilizadas no tratamento de patologias bucais são cirúrgicas, resultando em falhas de continuidade da mucosa bucal. A possibilidade de obtenção de epitélios transplantáveis, a partir do cultivo in vitro de células da mucosa bucal, abre novas perspectivas de utilização, não se restringindo somente ao seu local de origem, ou seja, a boca, mas também como material de reconstrução para outras regiões, tais como: uretra, córnea, superfície ocular e epitélio córneo-limbal. Os métodos utilizados para a obtenção dessas células ainda são controversos na literatura. Neste sentido, avaliamos e comparamos a eficiência de dois métodos, enzimático e explante, para a obtenção de queratinócitos de mucosa bucal humana. Os fragmentos utilizados para a obtenção dessas células foram obtidos durante procedimentos cirúrgicos de pacientes voluntários saudáveis. Os queratinócitos foram cultivados sobre uma camada de sustentação, feeder-layer, confeccionada com fibroblastos murinos irradiados (3T3 - Swiss albino). Neste estudo foram comparados: o tempo para a obtenção dos queratinócitos, o rendimento obtido entre os dois métodos, a duração da vida útil em cultura, a capacidade que estas células tiveram em formar um epitélio in vitro e a morfologia dos mesmos. Os resultados obtidos, na avaliação dos dois métodos, comprovaram a possibilidade de obtenção dos queratinócitos, a partir de um pequeno fragmento bucal, porém pode-se verificar que existem vantagens e restrições peculiares a cada um dos métodos estudados.
The therapeutic procedures frequently used in oral treatments for the pathological diseases are surgical, resulting in failures of the mucosal continuity.The possibility to obtain transplantable oral epithelia from an in vitro cell culture opens new utilization perspectives not only to where it comes from, but also as a reconstructive matherial for other parts of the human body, such as: urethra, epithelia corneo-limbal, cornea, ocular surface. Many researchers still use controversial methods for obtaining cells. It was therefore evaluated and compared the efficiency in both methods: enzimatic and direct explant to obtain oral keratinocytes from human oral mucosa. Fragments of intra oral epithelial tissues from healthy human subjects, undergoing dental surgeries, were donated to the research project. The keratinocytes were cultivated over a feeder-layer from a previously irradiated 3T3 Swiss albino fibroblasts. In this study it was compared the time needed in the cell obtaintion, the best cell amount between both methods, the life-span, the cell capacity to form an in vitro epithelia and its morphologic structure. The results in the accessment of both methods have shown the possibility to obtain keratinocytes from a small oral fragment, but at the same time we may verify the advantages and peculiar restrictions for each one of both analyzed methods.
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Geyer, Simone [Verfasser], and S. [Akademischer Betreuer] Scholpp. "Establishment of a three-dimensional cell culture system to study tubular structures - A comparative study of neuronal differentiation in zebrafish and in 2D and 3D zebrafish primary cell culture / Simone Geyer. Betreuer: S. Scholpp." Karlsruhe : KIT-Bibliothek, 2015. http://d-nb.info/1112224580/34.

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Книги з теми "Primary cell culture of bivalve":

1

Schantz, Jan-Thorsen. A manual for primary human cell culture. Hackensack, NJ: World Scientific, 2004.

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Koller, Manfred R. Human Cell Culture: Volume IV: Primary Hematopoietic Cells. Dordrecht: Kluwer Academic Publishers, 2002.

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R, Koller Manfred, Palsson Bernhard, and Masters John R. W, eds. Primary mesenchymal cells. Dordrecht: Kluwer Academic Publishers, 2001.

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R, Koller Manfred, Palsson Bernhard, and Masters J. R. W, eds. Primary hematopoietic cells. Dordrecht: Kluwer Academic Publishers, 1999.

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5

1936-, Neumann K. H., Barz W. 1935-, and Reinhard Ernst 1926-, eds. Primary and secondary metabolism of plant cell cultures. Berlin: Springer-Verlag, 1985.

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W, Masters John R., ed. Human cancer in primary culture: A handbook. Dordrecht: Kluwer Academic Publishers, 1991.

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J, Schripsema, and Verpoorte R, eds. Primary and secondary metabolism of plant cell cultures III. Dordrecht: Kluwer Academic Publishers, 1995.

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8

Yang, Phillip Chung-Ming. Hypertrophic response in primary single-cell culture of adult rat myocardial cells. [New Haven: s.n.], 1989.

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(Editor), F. Koller, B. Palsson (Editor), and J. R. Masters (Editor), eds. Human Cell Culture: Primary Mesenchymal Cells (Human Cell Culture, Volume 5) (Human Cell Culture). Springer, 2000.

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Human Cell Culture: Volume IV: Primary Hematopoietic Cells (Human Cell Culture). Springer, 1999.

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Частини книг з теми "Primary cell culture of bivalve":

1

O’Sullivan, Finbar, Paula Meleady, Shirley McBride, and Martin Clynes. "Primary Culture." In Animal Cell Culture Techniques, 115–28. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-80412-0_8.

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2

Martin, Bernice M. "Primary Cell Culture." In Tissue Culture Techniques, 113–36. Boston, MA: Birkhäuser Boston, 1994. http://dx.doi.org/10.1007/978-1-4612-0247-9_5.

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Gooch, Jan W. "Primary Cell Culture." In Encyclopedic Dictionary of Polymers, 917. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_14559.

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Milan, K. L., Goutham V. Ganesh, Dhamodharan Umapathy, Md Enamul Hoque, and K. M. Ramkumar. "Primary Cell Culture." In Advanced Mammalian Cell Culture Techniques, 18–20. Boca Raton: CRC Press, 2023. http://dx.doi.org/10.1201/9781003397755-6.

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Larsen, Therese Juhlin, Naja Zenius Jespersen, and Camilla Scheele. "Adipogenesis in Primary Cell Culture." In Brown Adipose Tissue, 73–84. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/164_2018_142.

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6

Darbinyan, Armine, Rafal Kaminski, Martyn K. White, Paul D. Pozniak, Nune Darbinian, and Kamel Khalili. "Isolation and Propagation of Primary Human and Rodent Embryonic and." In Neuronal Cell Culture, 51–61. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1437-2_5.

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Ng, Kee Woei, Mohan Chothirakottu Abraham, David Tai Wei Leong, Chris Morris, and Jan-Thorsten Schantz. "Primary Culture of Specific Cell Types and the Establishment of Cell Lines." In Animal Cell Culture, 205–30. Chichester, UK: John Wiley & Sons, Ltd, 2011. http://dx.doi.org/10.1002/9780470669815.ch7.

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Terrance, Mystica, Tarini Gunawardena, Hong Ouyang, Julie Avolio, Wenming Duan, Sowmya Thanikachalam, and Theo J. Moraes. "Primary Human Nasal Epithelial Cell Culture." In Methods in Molecular Biology, 213–23. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3507-0_13.

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Neumann, Karl-Hermann, Ashwani Kumar, and Jafargholi Imani. "Primary Metabolism." In Plant Cell and Tissue Culture – A Tool in Biotechnology, 209–31. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-49098-0_9.

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Neumann, Karl-Hermann, Jafargholi Imani, and Ashwani Kumar. "Primary Metabolism." In Plant Cell and Tissue Culture - A Tool in Biotechnology, 161–79. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-93883-5_9.

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Тези доповідей конференцій з теми "Primary cell culture of bivalve":

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Yong Jiang, Zhe Sun, and Tong-cun Zhang. "The primary culture of vascular endothelial cell in rat." In 2011 International Symposium on Information Technology in Medicine and Education (ITME 2011). IEEE, 2011. http://dx.doi.org/10.1109/itime.2011.6132114.

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Zhilnikova, M. V., O. S. Troitskaya, D. D. Novak, A. A. Nushtaeva, and O. A. Koval. "ESTABLISHMENT AND CHARACTERIZATION OF PRIMARY UVEAL MELANOMA CELL CULTURE." In X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-320.

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Анотація:
The primary human uveal melanoma (UM) cell culture was established and key surface and intracellular markers of oncotransformed melanocytes were characterized. Based on the primary cell culture, genetically modified cell culture of UM expressing fluorescent protein mKate2 and luciferase Luc2 was created.
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Shimba, Kenta, Atsushi Saito, Akimasa Takeuchi, Yuzo Takayama, Kiyoshi Kotani, and Yasuhiko Jimbo. "Bidirectional synaptic connection between primary and stem cell-derived neurons in co-culture device." In 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2013. http://dx.doi.org/10.1109/embc.2013.6611087.

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Eric, Sceusi, Asif Rashid, Yunfei Zhou, Shaija Samuel, Fan Fan, Ling Xia, Xiang-Cang Ye, et al. "Abstract 5199: Primary culture of human midgut carcinoid cell lines and isolation of a putative carcinoid stem cell population." In Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.am2011-5199.

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Simental, Alfred, Steve Lee, Pedro A. De Andrade Filho, Nathaniel R. Peterson, Saied Mirshahidi, Penelope Duerksen-Hughes, and Xiangpeng Yuan. "Abstract 60: Characterization of papillary thyroid carcinoma primary cell culture derived cancer stem-like cells." In Abstracts: AACR-AHNS Head and Neck Cancer Conference: Optimizing Survival and Quality of Life through Basic, Clinical, and Translational Research; April 23-25, 2017; San Diego, CA. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1557-3265.aacrahns17-60.

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Praveen, Kesavan Nair, Nicole Streiner, Martin Vo, Kenna Anderes, Koichi Yokota, and Takeshi Ikeya. "Abstract 5270: Evaluation of Cell-able spheroid culture system for culturing patient derived primary tumor cells." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-5270.

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Goji, Hiroshi, Manami Shimomura, Yasushi Uemura, Tetsuya Nakatsura, M. Mamunur Rahman, and Manabu Itoh. "Abstract 329: Establishment of three-dimensional primary tumor cell culture method and novel drug sensitivity test." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-329.

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Schlaermann, Philipp, and Thomas F. Meyer. "Abstract 2028: A novel human gastric primary cell culture system for modelling Helicobacter pylori infection in vitro." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-2028.

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Muñoz, Juan José Augusto Moyano, Sandra A. Drigo, Tiago Goss, Hellen Kuasne, Fabio Marchi, Gustavo Guimaraes, Ademar Lopes, Cristovam S. Neto, and Silvia R. Rogatto. "Abstract 3248: Establishment and characterization of primary penile carcinoma cell culture versus xenograft by using transcriptome analysis." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-3248.

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Shimomura, Manami, Ruriko Sakamoto, Kaori Yoshida, Ikuyo Ueda, Masahito Yonemura, Yoshihisa Shimada, and Tetsuya Nakatsura. "Abstract 5437: Novel drug sensitivity test using three-dimensional primary lung cancer cell culture on microfabricated surface." In Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.am2011-5437.

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Звіти організацій з теми "Primary cell culture of bivalve":

1

Heifetz, Yael, and Michael Bender. Success and failure in insect fertilization and reproduction - the role of the female accessory glands. United States Department of Agriculture, December 2006. http://dx.doi.org/10.32747/2006.7695586.bard.

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Анотація:
The research problem. Understanding of insect reproduction has been critical to the design of insect pest control strategies including disruptions of mate-finding, courtship and sperm transfer by male insects. It is well known that males transfer proteins to females during mating that profoundly affect female reproductive physiology, but little is known about the molecular basis of female mating response and no attempts have yet been made to interfere with female post-mating responses that directly bear on the efficacy of fertilization. The female reproductive tract provides a crucial environment for the events of fertilization yet thus far those events and the role of the female tract in influencing them are poorly understood. For this project, we have chosen to focus on the lower reproductive tract because it is the site of two processes critical to reproduction: sperm management (storage, maintenance, and release from storage) and fertilization. E,fforts during this project period centered on the elucidation of mating responses in the female lower reproductive tract The central goals of this project were: 1. To identify mating-responsive genes in the female lower reproductive tract using DNA microarray technology. 2. In parallel, to identify mating-responsive genes in these tissues using proteomic assays (2D gels and LC-MS/MS techniques). 3. To integrate proteomic and genomic analyses of reproductive tract gene expression to identify significant genes for functional analysis. Our main achievements were: 1. Identification of mating-responsive genes in the female lower reproductive tract. We identified 539 mating-responsive genes using genomic and proteomic approaches. This analysis revealed a shift from gene silencing to gene activation soon after mating and a peak in differential gene expression at 6 hours post-mating. In addition, comparison of the two datasets revealed an expression pattern consistent with the model that important reproductive proteins are pre-programmed for synthesis prior to mating. This work was published in Mack et al. (2006). Validation experiments using real-time PCR techniques suggest that microarray assays provide a conservativestimate of the true transcriptional activity in reproductive tissues. 2.lntegration of proteomics and genomics data sets. We compared the expression profiles from DNA microarray data with the proteins identified in our proteomic experiments. Although comparing the two data sets poses analyical challenges, it provides a more complete view of gene expression as well as insights into how specific genes may be regulated. This work was published in Mack et al. (2006). 3. Development of primary reproductive tract cell cultures. We developed primary cell cultures of dispersed reproductive tract cell types and determined conditions for organ culture of the entire reproductive tract. This work will allow us to rapidly screen mating-responsive genes for a variety of reproductive-tract specifi c functions. Scientific and agricultural significance. Together, these studies have defined the genetic response to mating in a part of the female reproductive tract that is critical for successful fertllization and have identified alarge set of mating-responsive genes. This work is the first to combine both genomic and proteomic approaches in determining female mating response in these tissues and has provided important insights into insect reproductive behavior.

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