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Добірка наукової літератури з теми "Plants Effect of stress on"

Автор: Grafiati

Опубліковано: 4 червня 2021

Оновлено: 27 липня 2024

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Статті в журналах з теми "Plants Effect of stress on"

Arora, Rajeev, Dharmalingam S. Pitchay, and Bradford C. Bearce. "EFFECT OF WATER STRESS ON HEAT STRESS TOLERANCE IN GERANIUM." HortScience 31, no. 6 (October 1996): 915A—915. http://dx.doi.org/10.21273/hortsci.31.6.915a.

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This study evaluated the effect of reversible water stress on heat stress tolerance (HST) in greenhouse-grown geraniums. Water stress was imposed by withholding irrigation until pots reached ≈30% (by weight) of well-watered (control) plant pots, and maintaining this weight for 7 days. Control plants were watered to just below field capacity, every other day. Leaf xylem water potential (LXWP, MPa), leaf-relative water content (LRWC,%), media water content (MWC, % fresh weight), and heat stress tolerance (HST, LT50) were determined for control and stressed plants. HST (LT50), defined as temperature causing half-maximal percent injury, was based on electrolyte leakage from leaf disks subjected to 25 to 60C. Control-watering was restored in stressed plants and above measurements made after 7 days of recovery. Data indicate: 1) LXWP, LRWC, and MWC in control and stressed plants were –0.378 and –0.804 MPa, 92.31% and 78.69% and 82.86% and 15.5%, respectively; 2) HST increased significantly in stressed as compared to control plants (LT50 of 55C vs. 51C); 3) control plants were near maximally injured by 53C treatment and sustained more than 3-fold greater injury than stressed plants at 53C. In recovered plants, LXWP and RWC reversed back to control levels, paralleled by loss of higher HST.

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Gupta, Sonal, and Ashwini A. Waoo. "Effect of salinity stress on phytochemical characteristics of Centella asiatica." Journal of Applied and Natural Science 14, no. 2 (June 18, 2022): 684–91. http://dx.doi.org/10.31018/jans.v14i2.3387.

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Salinity is one of the predominant abiotic stresses which affects plant growth by inducing excessive production of reactive oxygen species (ROS) that leads to oxidative damage of plant cells. Plants alleviate salinity stress by regulating intracellular concentrations of various phytochemicals like phenol, tannin, antioxidants, etc. The present work aimed to study the impact of salt stress on the production of various phytochemicals like phenol, tannin, flavonoids, antioxidants, total protein content, etc. The Salt stress response of the test plant Centella asiatica was studied by irrigating variant concentrations (50mM, 100mm, 150mm, 200mM, 250mm) of salt (NaCl). The phytochemical activity of the plants grown under salinity stress was estimated by using an appropriate biochemical assay. Comparative analysis of the photochemical activity of the test plants in comparison with the control revealed that various phytochemicals were increased in response to salt stress. Salt stress increased the levels of antioxidants from 10.79 to 14.31 μg/ml), phenol from 30.8 to 43.3 in μg/ml, flavonoids (from 490 to 683.33 in μg/ml), tannin from 55.5 to 64.5 in μg/ml, and proteins from 5720 to 6080 in μg/ml in the C. asiatica plants. To sum up, salt stress elicited phytochemical accumulation in the C. asiatica plant, thereby improving the plant's growth by enhancing its resistance to salt stress. This finding may play an important role in the sustainable cultivation of commercially important crops like C. asiatica.

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Shevchenko, A. V., I. G. Budzanivska, T. P. Shevchenko, and V. P. Polischuk. "Stress caused by plant virus infection in presence of heavy metals." Plant Protection Science 38, SI 2 - 6th Conf EFPP 2002 (December 31, 2017): 455–57. http://dx.doi.org/10.17221/10522-pps.

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Due to increased heavy metal content in Ukrainian soils, purpose of the work was to study relations between presence of heavy metals in soil and their effect on development of phytoviral infection. Experiments were conducted in Nicotiana tabacum – Potato virus X model system. Soluble salts of Cu, Zn and Pb were deposited in soil separately at the limiting concentrations simultaneously with virus infection of plants. Infected plants grown on usual soil showed symptoms of disease on 16 dpi as well as plants grown on soil with metals deposited. Contrary, combined effect of heavy metals and virus infection caused an increase of chlorophyll content comparing with control plants, therefore effect of heavy metals partially compensated the effect of virus infection on experimental plants.

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Krček, M., P. Slamka, K. Olšovská, M. Brestič, and M. Benčíková. "Reduction of drought stress effect in spring barley (Hordeum vulgare L.) by nitrogen fertilization." Plant, Soil and Environment 54, No. 1 (January 14, 2008): 7–13. http://dx.doi.org/10.17221/2781-pse.

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An effect of nitrogen rates (0.0 g, 1.0 g, 2.0 g N per pot) on NRA (nitrate reductase activity) in leaves of spring barley (cultivar Kompakt) was investigated in a pot experiment. Plants were grown under optimum moisture regime and drought stress was induced during the growth stages of tillering, shooting and earing. Before and after respective stress period plants were grown under optimal water regime. In all the fertilized and unfertilized treatments, NRA was significantly higher under optimal water regime than in drought stress conditions. Nitrogen fertilization alleviated adverse effects of drought stress on the yields of grain; the rate of 1 g N per pot increased the grain yield of plants stressed during tillering 3.73 times compared to unfertilized and stressed treatment. When the stress was induced during shooting or earing grain yields declined by over 50% compared to optimal water regime; when compared with stressed and unfertilized treatment, the rate of 1 g N however increased yield by 29% (stress at shooting) and 55% (stress at earing). NRA values were significantly higher when plants were grown under optimum water regime than under stress conditions as well as when fertilized with nitrogen compared to unfertilized control both under optimum water regime and drought stress.

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Ali-Ahmad, M., and S. M. Basha. "Effect of Water Stress on Composition of Peanut Leaves." Peanut Science 25, no. 1 (January 1, 1998): 31–34. http://dx.doi.org/10.3146/i0095-3679-25-1-8.

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Abstract Water stress was induced in peanut (Arachis hypogaea L. cv. Marc 1) plants by withholding water for 5 to 20 d. Leaves from the water-stressed plants were analyzed to determine the effect of water stress on amino acids, sugars, protein content, and polypeptide composition of peanut plants. The results showed that the total protein content of the leaves significantly increased when peanut plants were subjected to water stress for 5 to 20 d as compared to irrigated controls. Analysis of the leaf protein by SDS polyacrylamide gel electrophoresis showed higher levels of polypeptides in stressed leaves compared to the control leaves. Peanut leaves from water-stressed plants also showed higher amounts of free amino acids and soluble sugars as compared to the irrigated plants. Thus, water stress enhanced accumulation of proteins, free amino acids, and soluble sugars in the peanut plants.

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Handayani, Tri, and Kazuo Watanabe. "The combination of drought and heat stress has a greater effect on potato plants than single stresses." Plant, Soil and Environment 66, No. 4 (April 30, 2020): 175–82. http://dx.doi.org/10.17221/126/2020-pse.

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Several research groups have examined the effects of drought stress and heat stress on potato, but few investigations of the effects of combined drought-heat stress have been reported. Using five potato lines, the potato plants’ responses to drought stress, heat stress, as well as combined drought-heat stress were studied, to get the insight in phenotypic shift due to abiotic stresses. The experiment was conducted as a growth room experimental under non-stress and abiotic stresses (drought, heat, and combined drought-heat) conditions. The results demonstrated that potato plants responded to the abiotic stresses by decreasing their plant height, leaf size, cell membrane stability, and relative water content (RWC). However, increasing their leaf chlorophyll content under drought and combined drought-heat stresses. Generally, the combined drought-heat stress had a greater effect on the tested traits. The potato line L1 (84.194.30) showed the lowest level of wilting in all three types of abiotic stress, supported by a small RWC change compared to the control condition; L1 is thus considered relatively tolerant to abiotic stress. The potato lines’ different responses to each type of abiotic stress indicate that the potato lines have different levels of sensitivity to each abiotic stress.

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Asadova, B. "Salinity Factor Effect on Barley Seedlings Incubation." Bulletin of Science and Practice 8, no. 1 (January 15, 2022): 81–85. http://dx.doi.org/10.33619/2414-2948/74/11.

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Stress factors limit the development of living organisms, especially plants, and reduce their productivity. In this regard, the study of the effects of stress factors on plants and the discovery of adaptation mechanisms play an important role in the regulation of stress in the cell. From a biological point of view, stress is considered to be any change in the external environment that impairs the normal development of the plant or changes it in a negative direction. Stresses cause changes in the physiological activity of plants, weaken the process of biosynthesis in the cell, disrupt normal life and ultimately can cause plant death.

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Thakur, Jaya, and Bharat Shinde. "Effect of water stress and AM fungi on the growth performance of pea." International Journal of Applied Biology 4, no. 1 (June 29, 2020): 36–43. http://dx.doi.org/10.20956/ijab.v4i1.9446.

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The study was conducted to determine the effect of arbuscular mycorrhizal (AM) fungi inoculation on growth of pea grown under water stressed pot culture conditions. Water stress was given to the pea plants after 30 days at the interval of 4, 8 and 12 days. The data was collected at an interval of 15 days. Three replicates of each set were maintained. . The mixture of AM fungi used for current experiment included the species of Acaulospora denticulata, A. gerdemannii, Glomus macrocarpum, G. maculosum, G. fasciculatum and Scutellospora minuta. The mycorrhizal plants have shown more shoot and root length as compared to the control plants. The height of shoot and root was significantly decreased with the increase in drought stress. Mycorrhizal plants with low water stress showed enhanced shoot and root length than high water stress. The mycorrhizal plants have shown more number of leaves than control plants during drought stress. The number of leaves significantly reduced with the increase in drought stress. The leaves produced by the control plants were comparatively smaller than those of mycorrhizal plants. The dry weight of root and shoot of both control and mycorrhizal plants decreased with the increase in water stress. Mycorrhizal plants showed more dry weight of shoot and root as compared to control plants. Plants inoculated with AM fungi produce more dry weight than the control plants. The fresh weight of both control and mycorrhizal plants has been decreased with the increase in water stress interval and also the fresh weight of root and shoot was observed higher in mycorrhizal plants as compared to those of control plants.

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Kaňová, D., and E. Kula. "The effect of stress factors on birch Betula pendula Roth." Journal of Forest Science 50, No. 9 (January 11, 2012): 399–404. http://dx.doi.org/10.17221/4636-jfs.

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In a controlled pot trial, plants of birch (Betula pendula Roth) were treated in six variants: acid watering (pH 3), acid watering with spraying, drought, ammonium sulphate fertilisation, ammonium sulphate fertilisation in combination with drought, and control. The response to the treatment with ammonium sulphate in terms of the increment was discordant as it increased the sensitivity of birch to frost. Drought had a negative effect on increments. A combination of ammonium sulphate and drought; drought; ammonium sulphate and sprayed acid watering delayed the shedding of leaves; this was due to a longer vegetation period, significantly higher nitrogen content in these variants, with the exception of drought.

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Filiptsova, Halina G., and Vladimir M. Yurin. "Physiological and biochemical mechanisms of plants resistance to oxidative stress under peptide elicitor AtPep1." Journal of the Belarusian State University. Biology, no. 3 (November 5, 2021): 38–46. http://dx.doi.org/10.33581/2521-1722-2021-3-38-46.

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The effect of the peptide elicitor AtPep1 on the resistance of soybean and pea plants to oxidative stress was studied. The concentration of the peptide 10– 9 mol/L has the maximum elicitor effect on these plants. It was shown that treatment of the aerial part of seedlings with this peptide leads to an increase in the activity of peroxidase and superoxide dismutase and a decrease in the level of lipid peroxidation products in plants under oxidative stress. Revealed effects cause an increase in the plants resistance to stress.

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Більше джерел

Дисертації з теми "Plants Effect of stress on"

Eakes, Donald Joseph. "Moisture stress conditioning, potassium nutrition, and tolerance of Salvia splendens 'Bonfire' to moisture stress." Diss., Virginia Polytechnic Institute and State University, 1989. http://hdl.handle.net/10919/54350.

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The objective of this study was to determine the leaf water relations, gas exchange, and growth of the bedding plant salvia Salvia splendens 'Bonfire‘ as influenced by moisture stress conditioning (MSC - exposing plants to 4 sublethal dry-down cycles) and potassium (K) nutrition. Plants were fertilized with one of six K rates: 25, 75, 150, 300, 450, and 600 ppm as KCl in experiment one. Seven weeks after seeding plants were subjected to MSC. MSC and increasing K rate resulted in osmotic adjustment and increased cellular turgor potentials. Foliar K content increased as osmotic potentials decreased due to treatment. Although there was no interaction, MSC and high K rates both reduced transpiration (E), leaf conductance (g₁), and daily gravimetric water loss during well watered conditions. Greatest shoot dry weight occurred for plants grown with 300 ppm K and no-MSC. Gas exchange of salvia as influenced by MSC during the onset of moisture stress was determined in experiment two. On day one following final irrigation, MSC plants had lower mid-day E, g₁, hourly gravimetric water loss per unit leaf area, and net photosynthesis (Pn) compared to controls, despite no differences in leaf water potential (ψ₁). Percentage of stomatal inhibition of Pn (SI) was greater for MSC plants than controls with no differences in mesophyllic resistance to CO₂ (r_m). On day two, MSC plants had greater Pn, E, g₁, and hourly gravimetric water loss per unit leaf area, while SI and r_m were lower than controls. MSC plants maintained positive Pn rates and turgor to lower ψ₁ than control plants. Water use efficiency (WUE) estimates for MSC plants were greater than for controls. Salvia plants were fertilized with 75, 300, or 600 ppm K to determine the influence of K rate on gas exchange during the onset of moisture stress in a third experiment. On day one following final irrigation, plants grown with K rates of 300 and 600 ppm had lower E, g₁, hourly gravimetric water loss per unit leaf area, and Pn compared to 75 ppm K plants. On day two, 600 ppm K plants had greater Pn, E, and g₁ as the experiment was terminated compared to plants grown with 75 ppm K, although ψ₁ was similar. Potassium rate had little affect on WUE.
Ph. D.

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Le, Fevre Ruth Elizabeth. "Phytate and plant stress responses." Thesis, University of Cambridge, 2014. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708218.

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Choudhury, Feroza Kaneez. "Rapid Metabolic Response of Plants Exposed to Light Stress." Thesis, University of North Texas, 2018. https://digital.library.unt.edu/ark:/67531/metadc1157543/.

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Environmental stress conditions can drastically affect plant growth and productivity. In contrast to soil moisture or salinity that can gradually change over a period of days or weeks, changes in light intensity or temperature can occur very rapidly, sometimes over the course of minutes or seconds. So, in our study we have taken an metabolomics approach to identify the rapid response of plants to light stress. In the first part we have focused on the ultrafast (0-90 sec) metabolic response of local tissues to light stress and in the second part we analyzed the metabolic response associated with rapid systemic signaling (0-12 min). Analysis of the rapid response of Arabidopsis to light stress has revealed 111 metabolites that significantly alter in their level during the first 90 sec of light stress exposure. We further show that the levels of free and total glutathione accumulate rapidly during light stress in Arabidopsis and that the accumulation of total glutathione during light stress is dependent on an increase in nitric oxide (NO) levels. We further suggest that the increase in precursors for glutathione biosynthesis could be linked to alterations in photorespiration, and that phosphoenolpyruvate could represent a major energy and carbon source for rapid metabolic responses. Taken together, our analysis could be used as an initial road map for the identification of different pathways that could be used to augment the rapid response of plants to abiotic stress. In addition, it highlights the important role of glutathione in initial stage of light stress response. Light-induced rapid systemic signaling and systemic acquired acclimation (SAA) are thought to play an important role in the response of plants to different abiotic stresses. Although molecular and metabolic responses to light stress have been extensively studied in local leaves, and to a lesser degree in systemic leaves, very little is known about the metabolic responses that occur in the different tissues that connect the local to the systemic leaves. These could be important in defining the specificity of the systemic response as well as in supporting the propagation of different systemic signals, such as the reactive oxygen species (ROS) wave. Here we report that local application of light stress to one rosette leaf resulted in a metabolic response that encompassed local, systemic and transport tissues (tissues that connect the local and systemic tissues), demonstrating a high degree of physical and metabolic continuity between different tissues throughout the plant. We further show that the response of many of the systemically altered metabolites could be associated with the function of the ROS wave, and that the level of eight different metabolites is altered in a similar way in all tissues tested (local, systemic, and transport tissues). These compounds could define a core metabolic signature for light stress that propagates from the local to the systemic leaves. Taken together, our findings suggest that metabolic changes occurring in cells that connect the local and systemic tissues could play an important role in mediating rapid systemic signaling and systemic acquired acclimation to light stress.

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Kalifa, Ali. "Salt stress, and phosphorus absorption by potato plants cv. 'Russet Burbank'." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp04/mq29727.pdf.

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Ingarfield, Patricia Jean. "Effect of water stress and arbuscular mycorrhiza on the plant growth and antioxidant potential of Pelargonium reniforme Curtis and Pelargonium sidoides DC." Thesis, Cape Peninsula University of Technology, 2018. http://hdl.handle.net/20.500.11838/2794.

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Thesis (MTech (Horticulture))--Cape Peninsula University of Technology, 2018.
Pelargoniums have been studied extensively for their medicinal properties. P. reniforme and P. sidoides in particular are proven to possess antimicrobial, antifungal and antibiotic abilities due to their high antioxidant potential from compounds isolated from their tuberous roots. These plants have now been added to the medicine trade market and this is now causing concern for conservationists and they are generally harvested from the wild populations. This study evaluated the effect of water stress alone and in conjunction with arbuscular mycorrhiza on two species of Pelargoniums grown in a soilless medium. The experiment consisted of five different watering regimes which were applied to one hundred plants of each species without inoculation with arbuscular mycorrhiza and to one hundred plants of each species in conjunction with inoculation with AM. All the plants in the experiment were fed with a half-strength, standard Hoagland nutrient solution at varying rates viz. once daily to pot capacity, every three days to pot capacity, every six days to pot capacity, every twelve days to pot capacity and every twenty-four days to pot capacity. The objectives of the study were to measure the nutrient uptake, SPAD-502 levels (chlorophyll production) and metabolite (phenolics) formation of both species, grown under various rates of irrigation and water stress, as well with or without the addition of arbuscular mycorrhiza at planting out. Each treatment consisted of 10 replicates. SPAD-502 levels were measured weekly using a hand held SPAD-502 meter. Determination of nutrient uptake of macronutrients N, K, P, Ca, Mg and Na and micronutrients Cu, Zn, Mn, Al and B were measured from dry plant material at the end of the experiment by Bemlab, 16 Van Der Berg Crescent, Gants Centre, Strand. Plant growth in terms of wet and dry shoot and root weight were measured after harvest. Determination of concentrations of secondary metabolites (phenolic compounds) were assayed and measured spectrophotometrically at the end of the experiment. The highest significant reading of wet shoot weight for P. reniforme was taken in treatments 1 and 2 with and without mycorrhiza i.e. WF1, WF1M, WF2 and WF2M, with the highest mean found in WF1 with no mycorrhiza. This indicates that under high irrigation AM plays no part in plant growth, possibly due to leaching. More research is necessary in this regard. With regard to wet root weight, this was found to be not significant in any of the treatments, other than the longest roots being found in WF4. Measurements for dry root weight showed that WF1,2,3 and 5 were the most significant at P≤ 0.001 significance, with the highest weight found at treatment being WF3 and WF3M. The highest mean of shoot length of the plants was measured in treatment WF2 at moderate watering, but no statistical difference was found with water application and mycorrhiza addition. Nutrient uptake was increased in P. sidoides in all the different watering levels in the experiment except in the uptake of Mg. AM inoculation showed an increase in the uptake of Ca, while absorption of N occurred at higher water availability. K uptake was enhanced by the addition of AM in high water availability and K utilisation decreased as water stress increased. Medium to low watering resulted in higher leaf content in P. sidoides while the interaction between water availability and AM inoculation increased chlorophyll production towards the end of the experiment.

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Zhou, Maoqian 1961. "Nitrogen fixation by alfalfa as affected by salt stress and nitrogen levels." Thesis, The University of Arizona, 1989. http://hdl.handle.net/10150/277231.

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The growth and Nitrogen fixation by one low salt tolerant alfalfa (Medicago sativa L.) and two germination salt tolerant selections inoculated with were investigated at two salt levels (0, -0.6 Mpa) and two N rates (1, 5ppm) using a system which automatically recirculates a nutrient solution. The high level of salinity (-0.6 Mpa osmotic potential of culture solution) resulted in substantial reduction in the N fixation percentage and total fixed N. The effect of salinity was more pronounced for later cuttings than for the earlier cutting. The N fixation percentages were substantially decreased by increasing N level and the reduction was enhanced by time. The N treatment levels did not exhibit a significant effect on total fixed N. Cultivars did not differ in either growth or N fixation. However, the interaction of N and salinity significantly decreased the percentage and amount of N fixation.

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Zegeer, Abreeza May 1956. "Interactions between saline stress and benzyladenine on chili peppers (Capsicum annuum L.)." Thesis, The University of Arizona, 1989. http://hdl.handle.net/10150/277069.

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Exogenous application of BA (0, 50, 100 mg ul--1) had no significant effects on tolerance of chili peppers to salt (--0.75 MPa NaCl:CaCl₂, 3:1, w/w) as measured by vegetative and reproductive weights, numbers of reproductive structures, transpiration and total chlorophyll. When peppers were applied with microliter amounts of ¹⁴C labelled benzyladenine (BA; 44,400 dpm 1⁻¹), BA was translocated primarily acropetally from the site of application. Regardless of application site, translocated BA was ported primarily to expanding leaves, and BA was more readily absorbed by leaf as opposed to stem surfaces. Exogenous application of BA (0, 50, 100 mg ul⁻¹) had no significant effects on tolerance of chili peppers to salt (-0.75 MPa NaCl:CaCl₂, 3:1, w/w) as measured by vegetative and reproductive weights, numbers of reproductive structures, transpiration and total chlorophyll.

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Wongareonwanakij, Sathaporn. "Effects of water stress and partial soil-drying on senescence of sunflower plants." Title page, contents and summary only, 1995. http://web4.library.adelaide.edu.au/theses/09A/09aw872.pdf.

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Bibliography: leaves 98-123. This thesis investigates the symptoms of leaf senescence in response to plant shoot water stress and demonstrates the effect of a non-hydraulic root signal in the senescence response of mature leaves of sunflower. The alleviation of the leaf soluble protein loss rate by excision of the root system in drying soil indicates that this signal originates in roots in dry soil and acts to promote protein loss.

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Attumi, Al-Arbe. "Effect of salt stress on phosphorus and sodium absorptions by soybean plants." Thesis, McGill University, 1997. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=20242.

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The radiotracer methodology was combined with the Hoagland solution culture of growing soybean in a greenhouse to investigate the absorptions of phosphorus (P), calcium (Ca), and sodium (Na) as a function of salinity. Salt stress was varied by using zero to 120 mM NaCl. The research was initiated because of a need to increase soybean production in the saline soils of the semi-arid regions of the world. Although P absorption increased with time at each concentration of NaCl, increasing its concentrations ([NaCl]) to 120 mM reduced P uptake considerably. The addition of inorganic P (Pi) to the salt medium improved P absorption significantly (P < 0.0001) in stem, petiole, and roots. Polynomial regressions showed the relationship between 22Na activity and [NaCl] for leaves and petiole to be cubic (R2 = 1) while in the stem a quadratic relationship prevailed. A maximum of P and Na absorption was observed at 40 mM NaCl. The relationship between 32P activity and increasing [NaCl] was linear for the roots (a positive slope) and the stem (a negative slope). 45Ca and 32P dual labelling part of the experiments failed to produce results because an unexpectedly high degree of tissue quenching which prevented from obtaining the minimum counting requirements for separation. Shoot fresh and dry weights decreased linearly with increasing [NaCl] as did the root fresh and dry weights. Leaf chlorophyll content during the last week of the final harvest showed a linear relationship with time. Chlorophyll increased with time linearly when the growth medium contained zero and 40 mM NaCl; whereas a negative slope was obtained for 80 and 120 mM NaCl. It seems that P fertilization of the soil could ameliorate the salt effect. 22 Na uptake results indicated that there is a mechanism for exclusion of Na from soybean plant parts.

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Maclear, Athlee. "Identification of cis-elements and transacting factors involved in the abiotic stress responses of plants." Thesis, Rhodes University, 2005. http://hdl.handle.net/10962/d1007236.

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Many stress situations limit plant growth, resulting in crop production difficulties. Population growth, limited availability and over-utilization of arable land, and intolerant crop species have resulted in tremendous strain being placed on agriculturalists to produce enough to sustain the world's population. An understanding of the principles involved in plant resistance to environmental stress will enable scientists to harness these mechanisms to create stress-tolerant crop species, thus increasing crop production, and enabling the farming of previously unproductive land. This research project uses computational and bioinformatics techniques to explore the promoter regions of genes, encoding proteins that are up- or down-regulated in response to specific abiotic stresses, with the aim of identifying common patterns in the cis-elements governing the regulation of these abiotic stress responsive genes. An initial dataset of fifty known genes encoding for proteins reported to be up- or down-regulated in response to plant stresses that result in water-deficit at the cellular level viz. drought, low temperature, and salinity, were identified, and a postgreSQL database created to store relevant information pertaining to these genes and the proteins encoded by them. The genomic DNA was obtained where possible, and the promoter and intron regions identified. The Neural Network Promoter Prediction (NNPP) software package was used to predict the transcription start signal (TSS) and the promoter searching software tool, TESS (Transcription Element Search Software) used to identify known and user-defined cis-elements within the promoter regions of these genes. Currently available promoter prediction software analysis tools are reported to predict one promoter per kilobase of DNA, whilst functional promoters are thought to only occur one in 30-40 kilobases, which indicates that a large perccntage of predictions are likely to be false positives (pedersen et. al., 1999). NNPP was chosen as it was rated as the highest performing promoter prediction software tool by Fickett and Hatzigeorgiou (1997) in a thorough review of eukaryotic promoter prediction algorithms, however results were less than promising as very few predicted TSS were identified in the area 50 bps up- and downstream of the gene start site, where biologically functional TSSs are known to occur (Reese, 2000; Fickett and Hatzigeorgiou, 1997). TESS results seemed to support the hypothesis that drought, low-temperature and high salinity plant stress response proteins have similar as-elements in their promoter regions, and suggested links to various other gene regulation mechanisms viz. gibberellin-, light-, auxin- and development-regulated gene expression, highlighting the vast complexity of plant stress response processes. Although far from conclusive, results provide a valuable basis for future comparative promoter studies that will attempt to deduce possible common transcriptional initiation of abiotic stress response genes.
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Книги з теми "Plants Effect of stress on"

M, Orcutt David, and Hale Maynard G, eds. The physiology of plants under stress. New York: Wiley, 1996.

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Sir, Fowden Leslie, Mansfield T. A, Stoddart John, and Rank Prize Funds, eds. Plant adaptation to environmental stress. London: Chapman & Hall, 1993.

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A, Khan Nafees, and Singh Sarvajeet, eds. Abiotic stress and plant responses. New Delhi: I.K. International Pub. House, 2008.

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Shabala, S. Plant stress physiology. Edited by C. A. B. International. Cambridge, MA: CABI, 2012.

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Kadukova, Jana. Phytoremediation and stress: Evaluation of heavy metal-induced stress in plants. Hauppauge, N.Y: Nova Science Publishers, 2010.

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K, Panda S., ed. Advances in stress physiology of plants. Jodhpur: Scientific Publishers (India), 2002.

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S, Basra Amarjit, ed. Stress-induced gene expression in plants. Chur, Switzerland: Harwood Academic Publishers, 1994.

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Haryana, Nikhil. Abiotic stress: New research. Hauppauge, N.Y: Nova Science Publisher's, Inc., 2011.

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Heinrich, Sandermann, ed. Molecular ecotoxicology of plants. Berlin: Springer, 2004.

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G, Alscher Ruth, and Cumming Jonathan R, eds. Stress responses in plants: Adaptation and acclimation mechanisms. New York: Wiley-Liss, 1990.

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Kaur, Harpreet, Renu Bhardwaj, Vinod Kumar, Anket Sharma, Ravinder Singh, and Ashwani Kumar Thukral. "Effect of pesticides on leguminous plants." In Legumes under Environmental Stress, 91–101. Chichester, UK: John Wiley & Sons, Ltd, 2015. http://dx.doi.org/10.1002/9781118917091.ch6.

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Ivanov, Anatoly A. "Response of Wheat Seedlings to Combined Effect of Drought and Salinity." In Stress Responses in Plants, 159–98. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-13368-3_7.

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Pérez-Pastor, Alejandro, M. Carmen Ruiz-Sánchez, and María R. Conesa. "Drought stress effect on woody tree yield." In Water Stress and Crop Plants, 356–74. Chichester, UK: John Wiley & Sons, Ltd, 2016. http://dx.doi.org/10.1002/9781119054450.ch22.

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Hajiboland, R. "Effect of Micronutrient Deficiencies on Plants Stress Responses." In Abiotic Stress Responses in Plants, 283–329. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4614-0634-1_16.

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Khalid, Muhammad Fasih, Iqra Zakir, Rashid Iqbal Khan, Sobia Irum, Samreen Sabir, Nishat Zafar, Shakeel Ahmad, Mazhar Abbas, Talaat Ahmed, and Sajjad Hussain. "Effect of Water Stress (Drought and Waterlogging) on Medicinal Plants." In Medicinal Plants, 169–82. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-19-5611-9_6.

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Srivastava, Kavita, Sachidanand Singh, Anupam Singh, Tanvi Jain, Rahul Datta, and Abhidha Kohli. "Effect of Temperature (Cold and Hot) Stress on Medicinal Plants." In Medicinal Plants, 153–68. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-19-5611-9_5.

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Koshita, Yoshiko. "Effect of Temperature on Fruit Color Development." In Abiotic Stress Biology in Horticultural Plants, 47–58. Tokyo: Springer Japan, 2014. http://dx.doi.org/10.1007/978-4-431-55251-2_4.

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Tripathi, Durgesh Kumar, Swati Singh, Shweta Singh, Devendra Kumar Chauhan, Nawal Kishore Dubey, and Rajendra Prasad. "Silicon as a beneficial element to combat the adverse effect of drought in agricultural crops." In Water Stress and Crop Plants, 682–94. Chichester, UK: John Wiley & Sons, Ltd, 2016. http://dx.doi.org/10.1002/9781119054450.ch39.

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Hemati, Arash, Ebrahim Moghiseh, Arian Amirifar, Morteza Mofidi-Chelan, and Behnam Asgari Lajayer. "Physiological Effects of Drought Stress in Plants." In Plant Stress Mitigators, 113–24. Singapore: Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-16-7759-5_6.

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Flowers, T. S., and A. R. Yeo. "Effects of Salinity on Plant Growth and Crop Yields." In Environmental Stress in Plants, 101–19. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-73163-1_11.

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Baranova, E. N. "The effect of edaphic stress factors on plant cell compartments." In IX Congress of society physiologists of plants of Russia "Plant physiology is the basis for creating plants of the future". Kazan University Press, 2019. http://dx.doi.org/10.26907/978-5-00130-204-9-2019-57.

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Arkhipova, T. N., and E. V. Martynenko. "The effect of hormone producing bacteria on plant growth and stress tolerance." In IX Congress of society physiologists of plants of Russia "Plant physiology is the basis for creating plants of the future". Kazan University Press, 2019. http://dx.doi.org/10.26907/978-5-00130-204-9-2019-48.

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Kreslavsky, V. D., A. Yu Khudyakova, and V. Yu Lyubimov. "The effect of the phytochrome system on the stress resistance of the photosynthetic apparatus." In IX Congress of society physiologists of plants of Russia "Plant physiology is the basis for creating plants of the future". Kazan University Press, 2019. http://dx.doi.org/10.26907/978-5-00130-204-9-2019-237.

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Ponomareva, M. L., and S. N. Ponomarev. "Features of adaptation to winter stress and the effect of proline accumulation in winter cereals." In IX Congress of society physiologists of plants of Russia "Plant physiology is the basis for creating plants of the future". Kazan University Press, 2019. http://dx.doi.org/10.26907/978-5-00130-204-9-2019-362.

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Evseeva, N. V., A. Yu Denisova, G. L. Burygin, N. N. Pozdnyakova, and O. V. Tkachenko. "Coinoculation effect of potato microclones by rhizosphere bacteria under osmotic stress in vitro." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.067.

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Popescu, Monica. "ASCOPHYLLUM NODOSUM SEAWEED EXTRACT EFFECT ON DROUGHT STRESS IN BEAN PLANTS." In 17th International Multidisciplinary Scientific GeoConference SGEM2017. Stef92 Technology, 2017. http://dx.doi.org/10.5593/sgem2017h/63/s25.017.

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Ozolina, N. V., V. V. Gurina, and I. S. Nesterkina. "The effect of different types of abiotic stress on the dynamics of the content of common sterols of beet tonoplast (Beta vulgaris L.)." In IX Congress of society physiologists of plants of Russia "Plant physiology is the basis for creating plants of the future". Kazan University Press, 2019. http://dx.doi.org/10.26907/978-5-00130-204-9-2019-324.

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Zeng, Lizhang, and Da Xing. "Alteration in delayed fluorescence characterize the effect of heat stress on plants." In Photonics Asia 2004, edited by Yun-Jiang Rao, Osuk Y. Kwon, and Gang-Ding Peng. SPIE, 2005. http://dx.doi.org/10.1117/12.572779.

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Lukatkin, A. S., D. I. Bashmakov, E. Sh Sharkaeva, and A. A. Lukatkin. "Determining the effectiveness of growth regulators in the analysis of the effects of stress factors on plants." In IX Congress of society physiologists of plants of Russia "Plant physiology is the basis for creating plants of the future". Kazan University Press, 2019. http://dx.doi.org/10.26907/978-5-00130-204-9-2019-264.

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Pozhvanov, G. A., E. I. Sharova, and S. S. Medvedev. "Redox-dependent reorganization of the actin cytoskeleton in the root of arabidopsis under stress and regulatory effects." In IX Congress of society physiologists of plants of Russia "Plant physiology is the basis for creating plants of the future". Kazan University Press, 2019. http://dx.doi.org/10.26907/978-5-00130-204-9-2019-357.

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Mosquna, Assaf, and Sean Cutler. Systematic analyses of the roles of Solanum Lycopersicum ABA receptors in environmental stress and development. United States Department of Agriculture, January 2016. http://dx.doi.org/10.32747/2016.7604266.bard.

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Drought and other abiotic stresses have major negative effects on agricultural productivity. The plant hormone abscisic acid (ABA) regulates many responses to environmental stresses and can be used to improve crop performance under stress. ABA levels rise in response to diverse abiotic stresses to coordinate physiological and metabolic responses that help plants survive stressful environments. In all land plants, ABA receptors are responsible for initiating a signaling cascade that leads to stomata closure, growth arrest and large-scale changes in transcript levels required for stress tolerance. We wanted to test the meaning of root derived ABA signaling in drying soil on water balance. To this end we generated transgenic tomato lines in which ABA signaling is initiated by a synthetic agonist- mandipropamid. Initial study using a Series of grafting experiments indicate that that root ABA signaling has no effect on the immediate regulation of stomata aperture. Once concluded, these experiments will enable us to systematically dissect the physiological role of root-shoot interaction in maintaining the water balance in plants and provide new tools for targeted improvement of abiotic stress tolerance in crop plants.

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Handa, Avtar K., Yuval Eshdat, Avichai Perl, Bruce A. Watkins, Doron Holland, and David Levy. Enhancing Quality Attributes of Potato and Tomato by Modifying and Controlling their Oxidative Stress Outcome. United States Department of Agriculture, May 2004. http://dx.doi.org/10.32747/2004.7586532.bard.

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General The final goal and overall objective of the current research has been to modify lipid hydroperoxidation in order to create desirable phenotypes in two important crops, potato and tomato, which normally are exposed to abiotic stress associated with such oxidation. The specific original objectives were: (i) the roles of lipoxygenase (LOX) and phospholipids hydroperoxide glutathione peroxidase (PHGPx) in regulating endogenous levels of lipid peroxidation in plant tissues; (ii) the effect of modified lipid peroxidation on fruit ripening, tuber quality, crop productivity and abiotic stress tolerance; (iii) the effect of simultaneous reduction of LOX and increase of PHGPx activities on fruit ripening and tuber quality; and (iv) the role of lipid peroxidation on expression of specific genes. We proposed to accomplish the research goal by genetic engineering of the metabolic activities of LOX and PHGPx using regulatable and tissue specific promoters, and study of the relationships between these two consecutive enzymes in the metabolism and catabolism of phospholipids hydroperoxides. USA Significant progress was made in accomplishing all objectives of proposed research. Due to inability to regenerate tomato plants after transforming with 35S-PHGPx chimeric gene construct, the role of low catalase induced oxidative stress instead of PHGPx was evaluated on agronomical performance of tomato plant and fruit quality attributes. Effects of polyamine, that protects DNA from oxidative stress, were also evaluated. The transgenic plants under expressing lipoxygenase (LOX-sup) were crossed with catalase antisense (CAT-anti) plants or polyamine over producing plants (SAM-over) and the lines homozygous for the two transgenes were selected. Agronomical performance of these line showed that low catalase induced oxidative stress negatively affected growth and development of tomato plants and resulted in a massive change in fruit gene expression. These effects of low catalase activity induced oxidative stress, including the massive shift in gene expression, were greatly overcome by the low lipoxygenase activity. Collectively results show that oxidative stress plays significant role in plant growth including the fruit growth. These results also for the first time indicated that a crosstalk between oxidative stress and lipoxygenase regulated processes determine the outcome during plant growth and development. Israel Regarding PHGPx, most of the study has concentrated on the first and the last specific objectives, since it became evident that plant transformation with this gene is not obvious. Following inability to achieve efficient transformation of potato and tomato using a variety of promoters, model plant systems (tobacco and potato cell cultures, tobacco calli and plantlets, and Arabidopsis) were used to establish the factors and to study the obstacles which prohibited the regeneration of plants carrying the genetic machinery for overproduction of PHGPx. Our results clearly demonstrate that while genetic transformation and over-expression of PHGPx occurs in pre-developmental tissue stage (cell culture, calli clusters) or in completed plant (Arabidopsis), it is likely that over-expression of this enzyme before tissue differentiation is leading to a halt of the regeneration process. To support this assumption, experiments, in which genetic engineering of a point-mutated PHGPx gene enable transformation and over-expression in plants of PhSPY modified in its catalytic site and thus inactive enzymatically, were successfully carried out. These combined results strongly suggest, that if in fact, like in animals and as we established in vitro, the plant PHGPx exhibits PH peroxidase activity, these peroxides are vital for the organisms developmental process.

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Kirova, Elisaveta. Effect of Nitrogen Nutrition Source on Antioxidant Defense System of Soybean Plants Subjected to Salt Stress. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, February 2020. http://dx.doi.org/10.7546/crabs.2020.02.09.

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Alchanatis, Victor, Stephen W. Searcy, Moshe Meron, W. Lee, G. Y. Li, and A. Ben Porath. Prediction of Nitrogen Stress Using Reflectance Techniques. United States Department of Agriculture, November 2001. http://dx.doi.org/10.32747/2001.7580664.bard.

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Commercial agriculture has come under increasing pressure to reduce nitrogen fertilizer inputs in order to minimize potential nonpoint source pollution of ground and surface waters. This has resulted in increased interest in site specific fertilizer management. One way to solve pollution problems would be to determine crop nutrient needs in real time, using remote detection, and regulating fertilizer dispensed by an applicator. By detecting actual plant needs, only the additional nitrogen necessary to optimize production would be supplied. This research aimed to develop techniques for real time assessment of nitrogen status of corn using a mobile sensor with the potential to regulate nitrogen application based on data from that sensor. Specifically, the research first attempted to determine the system parameters necessary to optimize reflectance spectra of corn plants as a function of growth stage, chlorophyll and nitrogen status. In addition to that, an adaptable, multispectral sensor and the signal processing algorithm to provide real time, in-field assessment of corn nitrogen status was developed. Spectral characteristics of corn leaves reflectance were investigated in order to estimate the nitrogen status of the plants, using a commercial laboratory spectrometer. Statistical models relating leaf N and reflectance spectra were developed for both greenhouse and field plots. A basis was established for assessing nitrogen status using spectral reflectance from plant canopies. The combined effect of variety and N treatment was studied by measuring the reflectance of three varieties of different leaf characteristic color and five different N treatments. The variety effect on the reflectance at 552 nm was not significant (a = 0.01), while canonical discriminant analysis showed promising results for distinguishing different variety and N treatment, using spectral reflectance. Ambient illumination was found inappropriate for reliable, one-beam spectral reflectance measurement of the plants canopy due to the strong spectral lines of sunlight. Therefore, artificial light was consequently used. For in-field N status measurement, a dark chamber was constructed, to include the sensor, along with artificial illumination. Two different approaches were tested (i) use of spatially scattered artificial light, and (ii) use of collimated artificial light beam. It was found that the collimated beam along with a proper design of the sensor-beam geometry yielded the best results in terms of reducing the noise due to variable background, and maintaining the same distance from the sensor to the sample point of the canopy. A multispectral sensor assembly, based on a linear variable filter was designed, constructed and tested. The sensor assembly combined two sensors to cover the range of 400 to 1100 nm, a mounting frame, and a field data acquisition system. Using the mobile dark chamber and the developed sensor, as well as an off-the-shelf sensor, in- field nitrogen status of the plants canopy was measured. Statistical analysis of the acquired in-field data showed that the nitrogen status of the com leaves can be predicted with a SEP (Standard Error of Prediction) of 0.27%. The stage of maturity of the crop affected the relationship between the reflectance spectrum and the nitrogen status of the leaves. Specifically, the best prediction results were obtained when a separate model was used for each maturity stage. In-field assessment of the nitrogen status of corn leaves was successfully carried out by non contact measurement of the reflectance spectrum. This technology is now mature to be incorporated in field implements for on-line control of fertilizer application.

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Christopher, David A., and Avihai Danon. Plant Adaptation to Light Stress: Genetic Regulatory Mechanisms. United States Department of Agriculture, May 2004. http://dx.doi.org/10.32747/2004.7586534.bard.

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Original Objectives: 1. Purify and biochemically characterize RB60 orthologs in higher plant chloroplasts; 2. Clone the gene(s) encoding plant RB60 orthologs and determine their structure and expression; 3. Manipulate the expression of RB60; 4. Assay the effects of altered RB60 expression on thylakoid biogenesis and photosynthetic function in plants exposed to different light conditions. In addition, we also examined the gene structure and expression of RB60 orthologs in the non-vascular plant, Physcomitrella patens and cloned the poly(A)-binding protein orthologue (43 kDa RB47-like protein). This protein is believed to a partner that interacts with RB60 to bind to the psbA5' UTR. Thus, to obtain a comprehensive view of RB60 function requires analysis of its biochemical partners such as RB43. Background & Achievements: High levels of sunlight reduce photosynthesis in plants by damaging the photo system II reaction center (PSII) subunits, such as D1 (encoded by the chloroplast tpsbAgene). When the rate of D1 synthesis is less than the rate of photo damage, photo inhibition occurs and plant growth is decreased. Plants use light-activated translation and enhanced psbAmRNA stability to maintain D1 synthesis and replace the photo damaged 01. Despite the importance to photosynthetic capacity, these mechanisms are poorly understood in plants. One intriguing model derived from the algal chloroplast system, Chlamydomonas, implicates the role of three proteins (RB60, RB47, RB38) that bind to the psbAmRNA 5' untranslated leader (5' UTR) in the light to activate translation or enhance mRNA stability. RB60 is the key enzyme, protein D1sulfide isomerase (Pill), that regulates the psbA-RN :Binding proteins (RB's) by way of light-mediated redox potentials generated by the photosystems. However, proteins with these functions have not been described from higher plants. We provided compelling evidence for the existence of RB60, RB47 and RB38 orthologs in the vascular plant, Arabidopsis. Using gel mobility shift, Rnase protection and UV-crosslinking assays, we have shown that a dithiol redox mechanism which resembles a Pill (RB60) activity regulates the interaction of 43- and 30-kDa proteins with a thermolabile stem-loop in the 5' UTR of the psbAmRNA from Arabidopsis. We discovered, in Arabidopsis, the PD1 gene family consists of II members that differ in polypeptide length from 361 to 566 amino acids, presence of signal peptides, KDEL motifs, and the number and positions of thioredoxin domains. PD1's catalyze the reversible formation an disomerization of disulfide bonds necessary for the proper folding, assembly, activity, and secretion of numerous enzymes and structural proteins. PD1's have also evolved novel cellular redox functions, as single enzymes and as subunits of protein complexes in organelles. We provide evidence that at least one Pill is localized to the chloroplast. We have used PDI-specific polyclonal and monoclonal antisera to characterize the PD1 (55 kDa) in the chloroplast that is unevenly distributed between the stroma and pellet (containing membranes, DNA, polysomes, starch), being three-fold more abundant in the pellet phase. PD1-55 levels increase with light intensity and it assembles into a high molecular weight complex of ~230 kDa as determined on native blue gels. In vitro translation of all 11 different Pill's followed by microsomal membrane processing reactions were used to differentiate among PD1's localized in the endoplasmic reticulum or other organelles. These results will provide.1e insights into redox regulatory mechanisms involved in adaptation of the photosynthetic apparatus to light stress. Elucidating the genetic mechanisms and factors regulating chloroplast photosynthetic genes is important for developing strategies to improve photosynthetic efficiency, crop productivity and adaptation to high light environments.

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Fromm, Hillel, and Joe Poovaiah. Calcium- and Calmodulin-Mediated Regulation of Plant Responses to Stress. United States Department of Agriculture, September 1993. http://dx.doi.org/10.32747/1993.7568096.bard.

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We have taken a molecular approach to clone cellular targets of calcium/calmodulin (Ca2+/CaM). A 35S-labeled recombinant CaM was used as a probe to screen various cDNA expression libraries. One of the isolated clones from petunia codes for the enzyme glutamate decarboxylase (GAD) which catalyzes the conversion of glutamate to g-aminobutyric acid (GABA). The activity of plant GAD has been shown to be dramatically enhanced in response to cold and heat shock, anoxia, drought, mechanical manipulations and by exogenous application of the stress phytohormone ABA in wheat roots. We have purified the recombinant GAD by CaM-affinity chromatography and studied its regulation by Ca2+/CaM. At a physiological pH range (7.0-7.5), the purified enzyme was inactive in the absence of Ca2+ and CaM but could be stimulated to high levels of activity by the addition of exogenous CaM (K0.5 = 15 nM) in the presence of Ca2+ (K 0.5 = 0.8 mM). Neither Ca2+ nor CaM alone had any effect on GAD activity. Transgenic tobacco plants expressing a mutant petunia GAD lacking the CaM-binding domain, or transgenic plants expressing the intact GAD were prepared and studied in detail. We have shown that the CaM-binding domain is necessary for the regulation of glutamate and GABA metabolism and for normal plant development. Moreover, we found that CaM is tightly associated with a 500 kDa GAD complex. The tight association of CaM with its target may be important for the rapid modulation of GAD activity by Ca2+ signaling in response to stresses.

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Seginer, Ido, Daniel H. Willits, Michael Raviv, and Mary M. Peet. Transpirational Cooling of Greenhouse Crops. United States Department of Agriculture, March 2000. http://dx.doi.org/10.32747/2000.7573072.bard.

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Background Transplanting vegetable seedlings to final spacing in the greenhouse is common practice. At the time of transplanting, the transpiring leaf area is a small fraction of the ground area and its cooling effect is rather limited. A preliminary modeling study suggested that if water supply from root to canopy is not limiting, a sparse crop could maintain about the same canopy temperature as a mature crop, at the expense of a considerably higher transpiration flux per leaf (and root) area. The objectives of this project were (1) to test the predictions of the model, (2) to select suitable cooling methods, and (3) to compare the drought resistance of differently prepared seedlings. Procedure Plants were grown in several configurations in high heat load environments, which were moderated by various environmental control methods. The difference between the three experimental locations was mainly in terms of scale, age of plants, and environmental control. Young potted plants were tested for a few days in small growth chambers at Technion and Newe Ya'ar. At NCSU, tomato plants of different ages and planting densities were compared over a whole growing season under conditions similar to commercial greenhouses. Results Effect of spacing: Densely spaced plants transpired less per plant and more per unit ground area than sparsely spaced plants. The canopy temperature of the densely spaced plants was lower. Air temperature was lower and humidity higher in the compartments with the densely spaced plants. The difference between species is mainly in the canopy-to-air Bowen ratio, which is positive for pepper and negative for tomato. Effect of cooling methods: Ventilation and evaporative pad cooling were found to be effective and synergitic. Air mixing turned out to be very ineffective, indicating that the canopy-to-air transfer coefficient is not the limiting factor in the ventilation process. Shading and misting, both affecting the leaf temperature directly, proved to be very effective canopy cooling methods. However, in view of their side effects, they should only be considered as emergency measures. On-line measures of stress: Chlorophyll fluorescence was shown to accurately predict photosynthesis. This is potentially useful as a rapid, non-contact way of assessing canopy heat stress. Normalized canopy temperature and transpiration rate were shown to correlate with water stress. Drought resistance of seedlings: Comparison between normal seedlings and partially defoliated ones, all subjected to prolonged drought, indicated that removing about half of the lowermost leaves prior to transplanting, may facilitate adjustment to the more stressful conditions in the greenhouse. Implications The results of this experimental study may lead to: (1) An improved model for a sparse canopy in a greenhouse. (2) A better ventilation design procedure utilizing improved estimates of the evaporation coefficient for different species and plant configurations. (3) A test for the stress resistance of transplants.

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Amir, Rachel, David J. Oliver, Gad Galili, and Jacline V. Shanks. The Role of Cysteine Partitioning into Glutathione and Methionine Synthesis During Normal and Stress Conditions. United States Department of Agriculture, January 2013. http://dx.doi.org/10.32747/2013.7699850.bard.

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The objective of this research is to study the nature of the competition for cysteine (Cys), the first organic sulfur-containing compound, between its two main metabolites, glutathione (GSH) and methionine (Met). GSH plays a central role in protecting plants during various stresses, while Met, an essential amino acid, regulates essential processes and metabolites in plant cells through its metabolite S-adenosyl-Met. Our results, which are based on flux analysis and measurements of Met- metabolites, show that the flux towards Met synthesis is high during non-stress conditions, however the flux is significantly reduced under stress conditions, when there is high synthesis of GSH. Under oxidative stress the expression level of the regulatory enzyme of Met synthesis, cystathionine g-synthase (CGS) was reduced. By using three different systems, we have found that that GSH down regulates the expression level of CGS, thus reducing Met synthesis. We have found that this regulation occurs at the post-transcriptional level, and further studies have shown that it occurs at post-translationaly. To reveal how oxidative stress affects the flux towards Met and GSH, flux analysis was performed. We have found that the level of Met is significantly reduced, while the level of glutathione significantly increases during stress. Under stress conditions most of the glutathione is converted from GSH to GSSG (the oxidised form of glutathione). These results suggest that under normal growth conditions, Cys is channelled towards both pathways to support GSH accumulation and the synthesis of growth-essential Met metabolites. However, during oxidative stress, when a high level of GSH is required to protect the plants, the levels of GSH increase while those of CGS are reduced. This reduction leaves more Cys available for GSH synthesis under stress conditions. In addition we have also studied the effects of high GSH level on the transcriptome profile. The analysis revealed that GSH affects the expression level of many major genes coding to enzymes or proteins associated with photosynthesis, starch degradation, hormone metabolism (especially genes associated with jasmonate), biotic stress (especially genes associated with PR-proteins), cytochrome P450 genes, regulation of transcription and signaling (especially genes associated with receptor kinases and calcium). These results suggest that indeed GSH levels affect different pathways and metabolites in plants.

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Wolf, Shmuel, and William J. Lucas. Involvement of the TMV-MP in the Control of Carbon Metabolism and Partitioning in Transgenic Plants. United States Department of Agriculture, October 1999. http://dx.doi.org/10.32747/1999.7570560.bard.

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The function of the 30-kilodalton movement protein (MP) of tobacco mosaic virus (TMV) is to facilitate cell-to-cell movement of viral progeny in infected plants. Our earlier findings have indicated that this protein has a direct effect on plasmodesmal function. In addition, these studies demonstrated that constitutive expression of the TMV MP gene (under the control of the CaMV 35S promoter) in transgenic tobacco plants significantly affects carbon metabolism in source leaves and alters the biomass distribution between the various plant organs. The long-term goal of the proposed research was to better understand the factors controlling carbon translocation in plants. The specific objectives were: A) To introduce into tobacco and potato plants a virally-encoded (TMV-MP) gene that affects plasmodesmal functioning and photosynthate partitioning under tissue-specific promoters. B) To introduce into tobacco and potato plants the TMV-MP gene under the control of promoters which are tightly repressed by the Tn10-encoded Tet repressor, to enable the expression of the protein by external application of tetracycline. C) To explore the mechanism by which the TMV-MP interacts with the endogenous control o~ carbon allocation. Data obtained in our previous project together with the results of this current study established that the TMV-MP has pleiotropic effects when expressed in transgenic tobacco plants. In addition to its ability to increase the plasmodesmal size exclusion limit, it alters carbohydrate metabolism in source leaves and dry matter partitioning between the various plant organs, Expression of the TMV-MP in various tissues of transgenic potato plants indicated that sugars and starch levels in source leaves are reduced below those of control plants when the TMV-MP is expressed in green tissue only. However, when the TMV-MP was expressed predominantly in PP and CC, sugar and starch levels were raised above those of control plants. Perhaps the most significant result obtained from experiments performed on transgenic potato plants was the discovery that the influence of the TMV-MP on carbohydrate allocation within source leaves was under developmental control and was exerted only during tuber development. The complexity of the mode by which the TMV-MP exerts its effect on the process of carbohydrate allocation was further demonstrated when transgenic tobacco plants were subjected to environmental stresses such as drought stress and nutrients deficiencies, Collectively, these studies indicated that the influence of the TMV-MP on carbon allocation L the result of protein-protein interaction within the source tissue. Based on these results, together with the findings that plasmodesmata potentiate the cell-to-cell trafficking of viral and endogenous proteins and nucleoproteins complexes, we developed the theme that at the whole plant level, the phloem serves as an information superhighway. Such a long-distance communication system may utilize a new class of signaling molecules (proteins and/or RNA) to co-ordinate photosynthesis and carbon/nitrogen metabolism in source leaves with the complex growth requirements of the plant under the prevailing environmental conditions. The discovery that expression of viral MP in plants can induce precise changes in carbon metabolism and photoassimilate allocation, now provide a conceptual foundation for future studies aimed at elucidating the communication network responsible for integrating photosynthetic productivity with resource allocation at the whole-plant level. Such information will surely provide an understanding of how plants coordinate the essential physiological functions performed by distantly-separated organs. Identification of the proteins involved in mediating and controlling cell-to-cell transport, especially at the companion cell-sieve element boundary, will provide an important first step towards achieving this goal.

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Jander, Georg, Gad Galili, and Yair Shachar-Hill. Genetic, Genomic and Biochemical Analysis of Arabidopsis Threonine Aldolase and Associated Molecular and Metabolic Networks. United States Department of Agriculture, January 2010. http://dx.doi.org/10.32747/2010.7696546.bard.

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Since the amino acids threonine and isoleucine can be limiting in mammalian diet and there is interest in increasing their abundance in certain crop plants. To meet this need, a BARD proposal was written with two main research objectives: (i) investigate new avenues for manipulating threonine and isoleucine content in plants and (ii) study the role of threonine aldolase in plant metabolism. Research conducted to meet these goals included analysis of the sub-cellular localization of threonine aldolase in the plant, analysis of metabolic flux in developing embryos, over- and under-expression of Arabidopsis threonine aldolases, and transcriptional and metabolic analysis of perturbations resulting from altered threonine aldolase expression. Additionally, the broader metabolic effects of increasing lysine biosynthesis were investigated. An interesting observation that came up in the course of the project is that threonine aldolase activity affects methionine gamma-lyase in Arabidopsis. Further research showed that threonine deaminase and methionine gamma-lyase both contribute to isoleucine biosynthesis in plants. Therefore, isoleucine content can be altered by manipulating the expression of either or both of these enzymes. Additionally, both enzymes contribute to the up to 100-fold increase in isoleucine that is observed in drought-stressed Arabidopsis. Toward the end of the project it was discovered that through different projects, both groups had been able to independently up-regulate phenylalanine accumulation by different mechanisms. The Galili lab transformed Arabidopsis with a feedbackinsensitive bacterial enzyme and the Jander lab found a feedback insensitive mutation in Arabidopsis arogenate dehydratase. Exchange of the respective plant lines has allowed a comparative analysis of the different methods for increasing phenylalanine content and the creation of double mutants. The research that was conducted as part of this BARD project has led to new insights into plant amino acid metabolism. Additionally, new approaches that were found to increase the accumulation of threonine, isoleucine, and phenylalanine in plants have potential practical applications. Increased threonine and isoleucine levels can increase the nutritional value of crop plants. Elevated isoleucine accumulation may increase the osmotic stress tolerance of plants. Up-regulation of phenylalanine biosynthesis can be used to increase the production of downstream higher-value plant metabolites of biofuel feed stocks.

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