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Статті в журналах з теми "Plant functional strategies":
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Yang, Yanzheng, Qiuan Zhu, Changhui Peng, Han Wang, and Huai Chen. "From plant functional types to plant functional traits." Progress in Physical Geography: Earth and Environment 39, no. 4 (May 7, 2015): 514–35. http://dx.doi.org/10.1177/0309133315582018.
Анотація:
Dynamic global vegetation models (DGVMs) typically track the material and energy cycles in ecosystems with finite plant functional types (PFTs). Increasingly, the community ecology and modelling studies recognize that current PFT scheme is not sufficient for simulating ecological processes. Recent advances in the study of plant functional traits (FTs) in community ecology provide a novel and feasible approach for the improvement of PFT-based DGVMs. This paper reviews the development of current DGVMs over recent decades. After characterizing the advantages and disadvantages of the PFT-based scheme, it summarizes trait-based theories and discusses the possibility of incorporating FTs into DGVMs. More importantly, this paper summarizes three strategies for constructing next-generation DGVMs with FTs. Finally, the method’s limitations, current challenges and future research directions for FT theory are discussed for FT theory. We strongly recommend the inclusion of several FTs, namely specific leaf area (SLA), leaf nitrogen content (LNC), carbon isotope composition of leaves (Leaf δ13C), the ratio between leaf-internal and ambient mole fractions of CO2 (Leaf Ci/Ca), seed mass and plant height. These are identified as the most important in constructing DGVMs based on FTs, which are also recognized as important ecological strategies for plants. The integration of FTs into dynamic vegetation models is a critical step towards improving the results of DGVM simulations; communication and cooperation among ecologists and modellers is equally important for the development of the next generation of DGVMs.
2
Shen, Yaou, Guangtang Pan, and Thomas Lübberstedt. "Haploid Strategies for Functional Validation of Plant Genes." Trends in Biotechnology 33, no. 10 (October 2015): 611–20. http://dx.doi.org/10.1016/j.tibtech.2015.07.005.
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Golluscio, Rodolfo A., and Osvaldo E. Sala. "Plant functional types and ecological strategies in Patagonian forbs." Journal of Vegetation Science 4, no. 6 (December 1993): 839–46. http://dx.doi.org/10.2307/3235623.
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Raevel, Valérie, Cyrille Violle, and François Munoz. "Mechanisms of ecological succession: insights from plant functional strategies." Oikos 121, no. 11 (March 13, 2012): 1761–70. http://dx.doi.org/10.1111/j.1600-0706.2012.20261.x.
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Adler, P. B., R. Salguero-Gomez, A. Compagnoni, J. S. Hsu, J. Ray-Mukherjee, C. Mbeau-Ache, and M. Franco. "Functional traits explain variation in plant life history strategies." Proceedings of the National Academy of Sciences 111, no. 2 (December 30, 2013): 740–45. http://dx.doi.org/10.1073/pnas.1315179111.
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Reich, P. B., I. J. Wright, J. Cavender‐Bares, J. M. Craine, J. Oleksyn, M. Westoby, and M. B. Walters. "The Evolution of Plant Functional Variation: Traits, Spectra, and Strategies." International Journal of Plant Sciences 164, S3 (May 2003): S143—S164. http://dx.doi.org/10.1086/374368.
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Butterfield, Bradley J., and John M. Briggs. "Regeneration niche differentiates functional strategies of desert woody plant species." Oecologia 165, no. 2 (August 5, 2010): 477–87. http://dx.doi.org/10.1007/s00442-010-1741-y.
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Xian, Ling, Jiao Yang, Samuel Wamburu Muthui, Wyckliffe Ayoma Ochieng, Elive Limunga Linda, and Junshuang Yu. "Which Has a Greater Impact on Plant Functional Traits: Plant Source or Environment?" Plants 13, no. 6 (March 21, 2024): 903. http://dx.doi.org/10.3390/plants13060903.
Анотація:
The deterioration of water quality caused by human activities has triggered significant impacts on aquatic ecosystems. Submerged macrophytes play an important role in freshwater ecosystem restoration. Understanding the relative contributions of the sources and environment to the adaptive strategies of submerged macrophytes is crucial for freshwater restoration and protection. In this study, the perennial submerged macrophyte Myriophyllum spicatum was chosen as the experimental material due to its high adaptability to a variable environment. Through conducting reciprocal transplant experiments in two different artificial environments (oligotrophic and eutrophic), combined with trait network and redundancy analysis, the characteristics of the plant functional traits were examined. Furthermore, the adaptive strategies of M. spicatum to the environment were analyzed. The results revealed that the plant source mainly influenced the operational pattern among the traits, and the phenotypic traits were significantly affected by environmental factors. The plants cultured in high-nutrient water exhibited a higher plant height, longer leaves, and more branches and leaves. However, their physiological functions were not significantly affected by the environment. Therefore, the adaptation strategy of M. spicatum to the environment mainly relies on its phenotypic plasticity to ensure the moderate acquisition of resources in the environment, thereby ensuring the stable and efficient operation of plant physiological traits. The results not only offered compelling evidence on the adaptation strategies of M. spicatum in variable environments but also provided theoretical support for the conservation of biodiversity and sustainable development.
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Buotte, Polly C., Charles D. Koven, Chonggang Xu, Jacquelyn K. Shuman, Michael L. Goulden, Samuel Levis, Jessica Katz, et al. "Capturing functional strategies and compositional dynamics in vegetation demographic models." Biogeosciences 18, no. 14 (July 30, 2021): 4473–90. http://dx.doi.org/10.5194/bg-18-4473-2021.
Анотація:
Abstract. Plant community composition influences carbon, water, and energy fluxes at regional to global scales. Vegetation demographic models (VDMs) allow investigation of the effects of changing climate and disturbance regimes on vegetation composition and fluxes. Such investigation requires that the models can accurately resolve these feedbacks to simulate realistic composition. Vegetation in VDMs is composed of plant functional types (PFTs), which are specified according to plant traits. Defining PFTs is challenging due to large variability in trait observations within and between plant types and a lack of understanding of model sensitivity to these traits. Here we present an approach for developing PFT parameterizations that are connected to the underlying ecological processes determining forest composition in the mixed-conifer forest of the Sierra Nevada of California, USA. We constrain multiple relative trait values between PFTs, as opposed to randomly sampling within the range of observations. An ensemble of PFT parameterizations are then filtered based on emergent forest properties meeting observation-based ecological criteria under alternate disturbance scenarios. A small ensemble of alternate PFT parameterizations is identified that produces plausible forest composition and demonstrates variability in response to disturbance frequency and regional environmental variation. Retaining multiple PFT parameterizations allows us to quantify the uncertainty in forest responses due to variability in trait observations. Vegetation composition is a key emergent outcome from VDMs and our methodology provides a foundation for robust PFT parameterization across ecosystems.
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Gong, Qi, Bin Wang, Xubiao Lu, Jiantao Tan, Yuke Hou, Taoli Liu, Yao-Guang Liu, and Qinlong Zhu. "Nicking Endonuclease-Mediated Vector Construction Strategies for Plant Gene Functional Research." Plants 9, no. 9 (August 25, 2020): 1090. http://dx.doi.org/10.3390/plants9091090.
Анотація:
Plant genetic engineering vectors, such as RNA interference (RNAi) and CRISPR/Cas9 vectors, are important tools for plant functional genomics. Efficient construction of these functional vectors can facilitate the study of gene function. Although some methods for vector construction have been reported, their operations are still complicated and costly. Here, we describe a simpler and low-cost vector construction method by nicking endonucleases-mediated DNA assembly (NEMDA), which uses nicking endonucleases to generate single-strand overhanging complementary ends for rapid assembly of DNA fragments into plasmids. Using this approach, we rapidly completed the construction of four RNAi vectors and a CRISPR/Cas9 knockout vector with five single-guide RNA (sgRNA)-expression cassettes for multiplex genome editing, and successfully achieved the goal of decreasing the expression of the target genes and knocking out the target genes at the same time in rice. These results indicate the great potential of NEMDA in assembling DNA fragments and constructing plasmids for molecular biology and functional genomics.
Дисертації з теми "Plant functional strategies":
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Santini, Gonzalez Bianca Ariana. "Plant functional traits and vegetation strategies." Thesis, University of Sheffield, 2015. http://etheses.whiterose.ac.uk/12119/.
Анотація:
In this thesis we tested the predictions from the CSR theory for the community membership. Predictions are that, in the absence of competition, species from all strategies (Competitors, Ruderals and Stress-tolerants) will persist in low-stress habitats, whereas in high-stress habitats, only species with Stress-tolerant traits will survive. CSR recognizes that species evolved similar traits to one universal stress. For this reason, we were interested in testing which strategies will survive in different sources of stress. Our results from field and greenhouse experiments suggest that CSR theory does not predict community membership from the initial stages of a plant life-cycle. Instead, we found that the habitat stress plays a major role in determining the species that are incorporated into a community. In this thesis we also used a trait-based approach to evaluate: 1) the relationship between key traits using annuals species, and 2) the links between genome size and phenotypic variation within species. Firstly, we studied the triangular relationship reported for seed mass and leaf area in woody species. These traits are involved in the plant reproduction strategy and plant water and energy-use. We found a triangular relationship in annuals species, suggesting that is conserved across groups (woody and annuals). We also found that the driver of this relationship is related to soil fertility. Finally, for the relationship between genome size and phenotypic variation within species, we found that larger genome species display higher variation in traits than small genome species. This can be a potential advantage in heterogeneous environments where the amount of phenotypic variation would allow the species to adapt to them.
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Nunes, Alice Maria Rodrigues. "Plant functional response to desertification and land degradation: contribution to restoration strategies." Doctoral thesis, Universidade de Aveiro, 2017. http://hdl.handle.net/10773/18814.
Анотація:
Doutoramento em Biologia e Ecologia das Alterações GlobaisÉ crucial entender o impacto das alterações climáticas nos ecossistemas áridos, pois estas poderão agravar a desertificação e degradação dos solos, comprometendo o funcionamento dos ecossistemas e os serviços a eles associados. Os atributos funcionais ligam as espécies aos processos do ecossistema, fornecendo um entendimento mecanicista da sua resposta ao clima. O principal objectivo desta tese foi modelar a resposta de ecossistemas áridos ao clima com base em atributos funcionais de plantas (AFP), usando um gradiente climático espacial para prever alterações no tempo. Pretendeu-se assim selecionar um indicador baseado em AFP para monitorizar os efeitos do clima, e contribuir para melhorar as estratégias de gestão e restauro de zonas áridas. Embora a maioria das métricas de diversidade funcional requeiram a quantificação dos AFP no campo, não existia consenso sobre qual o melhor método para ser usado à escala global. Comparámos diferentes métodos, e demonstrámos as vantagens do método dos quadrados pontuais na monitorização de fina-escala dos AFP em zonas áridas. Desconhecia-se quais os principais AFP que respondiam à aridez, o que é essencial para o seu uso como indicadores de alterações no ecossistema. Identificámos nove AFP que respondem à aridez. Esta afectou a respectiva média, e reduziu a diversidade funcional. O uso de gradientes climáticos no espaço para prever alterações no tempo, requer a comparação de ambos os padrões, para a respectiva validação. Verificámos que as variações climáticas entre anos afectam os AFP, indicando que as mudanças funcionais são transitórias. Contudo, a diversidade funcional diminuiu em condições climáticas mais limitantes. Assim, face a uma maior aridez, espera-se que os padrões de resposta no tempo convirjam para os verificados no espaço. Os AFP podem responder de forma mais previsível ao clima do que a diversidade de espécies. Desenvolvemos um indicador de diversidade funcional baseado em múltiplos AFP, que diminuiu de forma monotónica não-linear com a aridez, mostrando ter maior capacidade preditora da resposta ao clima do que a diversidade de espécies. Os factores biofísicos locais modulam o efeito do clima na vegetação. Verificámos que os factores topo-edáficos têm um papel chave nos AFP envolvidos na colonização por arbustos. Os factores climáticos pouco influíram, sugerindo que as alterações climáticas não promoverão a colonização por arbustos. Os AFP podem ser uma ferramenta essencial no restauro de zonas áridas. Apresentámos uma visão geral sobre projectos de restauro, mostrando a necessidade de uma melhor integração dos AFP no restauro de zonas áridas. Ao demonstrar que os AFP são indicadores consistentes do impacto do clima nos ecossistemas, contribuímos para melhorar as previsões dos efeitos das alterações climáticas nas zonas áridas, e desenvolvemos um indicador que pode ser usado para mapear áreas em risco de desertificação e degradação do solo, potencialmente a uma escala global.
It is crucial to anticipate the impacts of climate change on drylands, as it may aggravate desertification and land degradation, hampering ecosystems functioning and associated services. Functional traits determine species’ responses to environment, and their influence on ecosystem processes, thus providing a mechanistic tool to monitor ecosystems’ response to climate. The main aim of this thesis was to model the response of Mediterranean dryland ecosystems to climate, based on plant functional traits (PFT), using a spatial climatic gradient to predict changes over time. It aimed at selecting a traitbased indicator to track climate change effects on drylands, and contribute to improve land management and restoration strategies to mitigate land degradation. Although most trait-based metrics require the quantification of PFT in the field, there is no consensus about the best plant-sampling method to do it, to be used at a global scale. By comparing the performance of different methods, we demonstrated the advantages of the point-intercept method to perform fine-scale monitoring of PFT in drylands. Understanding which PFT respond to climate is essential to their use as indicators of ecosystems´ changes. Yet, this is not well established in drylands. We identified nine PFT responding to aridity. It affected PFT means, and reduced functional diversity. Studying climatic gradients in space to infer changes over time, requires a validation between the two. We found that inter-annual climatic fluctuations greatly affected PFT, indicating that functional changes are transitory. Yet, functional diversity was reduced under more limiting climatic conditions. Hence, we hypothesize that if drier conditions prevail in time, changes over time will approach those found along space. PFT are likely to respond in a more predictable way to environment than species diversity. We built a multi-trait functional diversity indicator, which showed a monotonic non-linear decrease with increasing aridity, responding in a more predictable way to climate than species diversity. Local biophysical factors modulate the effect of climate on plant communities. We explored the relative effect of these factors, and found that topo-edaphic factors played a major role shaping PFT associated to shrub encroachment. Climatic factors had a minor influence, suggesting that climate change will not promote shrub encroachment in Mediterranean drylands. PFT may be an important tool to improve dryland restoration. We provide a comprehensive overview of the current restoration practice in Mediterranean drylands, showing the need for a better integration of trait-based ecology into dryland restoration. By showing that PFT are consistent indicators of the impact of climate on dryland ecosystems, this work contributed to improve predictions on the effects of climate change on drylands, and enabled the development of a trait-based indicator which can be used to map areas at risk of desertification and land degradation, potentially at a global scale.
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Singhabahu, S. A. D. Sanjeewa. "Plant-based strategies aimed at expressing a functional human adenosine deaminase at high levels." Thesis, University of East London, 2012. http://roar.uel.ac.uk/1889/.
Анотація:
An inherited disorder, ADA deficiency, is a form of severe combined immunodeficiency, which is ultimately caused by an absence of adenosine deaminase (ADA), a key enzyme of the purine salvage pathway. The absence of ADA-activity in sufferers eventually results in a dysfunctional immune system due to the build-up of toxic metabolites. To date, this has been treated with mixed success, using PEG-ADA, made from purified bovine ADA coupled to polyethylene glycol. It is likely however, that an enzyme replacement therapy protocol based on recombinant human ADA would be a more effective treatment for this disease. Therefore, as a preliminary step to produce biologically active human ADA in transgenic tobacco plants and tobacco BY-2 cell suspensions a human ADA cDNA has been inserted into a plant expression vector under the control of the CaMV 35S promoter and terminator. In an attempt to maximise the yield various recombined gene constructs containing apoplast targeting sequences were tested along with different translational regulatory sequences such as TMV omega and RUBISCO untranslated regions. Tobacco plants and BY-2 cells transformed with cytosolic constructs showed levels of recombinant ADA of up to 80 ng mg-1 TSP. By comparison, transgenic calli expressing constructs containing apoplast-directing signals showed higher levels of recombinant ADA expression of up to 115 ng mg-1 TSP. The most significant ADA activities were measured in transgenic BY-2 cell suspensions, however. Where, incorporation of a signal for arabinogalactan addition at the C-terminus of the recombinant ADA gene, targeted for secretion, produced a maximum yield of approximately 13 mg L-1. Representing a 336-fold increase over ADA activities recorded in a BY-2 suspension transformed with a cytosolic counterpart.
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Rande, Hugo. "Effets du niveau de pollution métallique et des stratégies fonctionnelles sur les types d’effets en jeu dans les interactions entre plantes au niveau d’anciens sites miniers des Pyrénées." Electronic Thesis or Diss., Bordeaux, 2024. http://www.theses.fr/2024BORD0032.
Анотація:
Les interactions entre plantes dans les milieux impactés par les métaux/métalloïdes dépendent de nombreux facteurs et sont encore très peu connues. Tout d’abord elles semblent dépendre du niveau de pollution métallique du milieu, mais aussi des stratégies fonctionnelles végétales des plantes interagissant. D’autre part, les plantes peuvent avoir plusieurs types d’effets sur leur environnement proche, effets s’exprimant à des temporalités différentes. En effet, les plantes ont un effet immédiat via leur canopée et racines sur les ressources et le microclimat à proximité. Aussi, au cours d’une saison de végétation, les plantes peuvent avoir des effets liés à la production de la litière et sa décomposition dans les sols sous leur canopée. A plus long-terme, lorsque ce cycle de production/décomposition est répété au fil des années, les plantes vont avoir un effet lié à la dynamique de la matière organique dans les sols. Dans cette thèse, notre objectif principal était de différencier ces effets, et de comprendre comment les stratégies fonctionnelles végétales des plantes pouvaient influencer les différents effets en jeu le long de gradients de pollution métallique. Nous avons étudié ces effets pendant trois années (entre 2020 et 2022) dans une ancienne vallée minière des Pyrénées Ariégeoises (Sentein, France). Dans cette zone d’étude, nous avons étudié les interactions entre plantes par des méthodes observationnelles et de transplantations de cibles avec contrôle de la présence de canopée et/ou de la litière des plantes, sur trois sites d’étude : un terril avec une pollution homogène et deux zones de résidus miniers avec des pollutions hétérogènes créant un gradient de pollution. Le long des gradients étudiés, les effets de canopée et de prélèvement racinaire ont suivi l’Hypothèse du Gradient de Stress, passant de la compétition à la facilitation avec l’augmentation de la pollution. Cette facilitation était d’autant plus forte que les espèces produisant l’effet sont dites « exploitatrices » (en lien avec l’exploitation des ressources du sol et Leaf Economic Spectrum), et bénéficiait le plus aux plantes les moins tolérantes aux métaux. Les effets positifs étaient surtout liés à l’amélioration des conditions microclimatiques lors d’épisodes chauds et secs en été. Concernant les effets liés à la production et décomposition des litières, des effets négatifs sur les plantes cibles ont été démontrés, suggérant des effets dits d’allélopathie élémentaire, et liés à la forte concentration en éléments métalliques dans les litières en décomposition. Ces effets négatifs de litière étaient maximums dans les milieux les moins pollués où les plantes métallophytes accumulatrices (qui ont des fortes teneurs en métaux dans leurs feuilles) et les plantes moins tolérantes aux métaux interagissaient. Ils étaient particulièrement marqués pour les cibles sensibles à la pollution métallique. Les résultats de cette thèse donnent des pistes potentielles pour utiliser la facilitation dans un cadre de phyto-management de milieux pollués par les métaux/métalloïdes, en prenant en compte explicitement les stratégies fonctionnelles végétales des plantes en interactions et le niveau de pollution en jeu. Des résultats obtenus pendant la canicule de 2022 nous donnent aussi une bonne vision des évolutions attendues des différents effets impliqués dans les interactions entre plantes dans les écosystèmes métallifères dans un contexte de changement climatiquePlant-plant interactions have been overlooked in metal/metalloids-impacted environments and are likely driven by several factors whose influence is barely known. First, plant-plant interactions depend on the level of metal pollution, but also on the functional plant strategies of the interacting plants. Furthermore, plants can have several type of effects on their immediate environment, acting at different timescales. Plants canopy and roots have an instantaneous influence on the microclimate and available resources in their immediate vicinity. Then, during a growing season, the production of litter and its decomposition beneath their canopy can influence soil chemical and physical properties. In the longer term, when this cycle of litter production/decomposition is repeated over the years, the dynamics of the organic matter will influence soil conditions even more. In this thesis, our main objective was to delineate these effects, and to understand how plant functional strategies can influence these various effects along metal pollution gradients. We studied these effects during three consecutive years (from 2020 to 2022) in a former mining valley in the French Pyrenees (Sentein, Ariège, France). In this area, we studied interactions between plants using observational and target transplantation methods controlling for the presence of plant canopy and/or plant litter, in three study sites: a slag heap with homogeneous pollution and two mine tailings areas with heterogeneous pollution creating a gradient of pollution. Along these gradients, short-term canopy and root-uptake effects followed the Stress Gradient Hypothesis, switching from competition to facilitation as pollution increased. This facilitation was stronger when the species producing the effect were acquisitive (in relation with soil resources and the Leaf Economic Spectrum), and benefits more the low metal-tolerant plants. These positive effects were mainly due to the improvement of micro-climatic conditions during hot and dry episodes in summer. Concerning the effects linked to litter production and decomposition, negative effects on target plants were found, suggesting the so-called “elemental allelopathic” effects, in relation with the high concentration of metallic elements in the decomposing litter. These negative litter effects were more important in the least polluted environments, where metal-accumulating metallophyte plants (which have high concentration of metals in their leaves) and less metal-tolerant plants interacts. They were particularly marked for targets sensitive to metal pollution. The results of this thesis give important perspectives regarding the use of facilitation for the phyto-management of metals/metalloids-polluted environments, given that the functional strategies of interacting plants and the level of pollution involved are explicitly considered. Additionnaly, the results obtained during the 2022 heatwave provide useful insights regarding the expected evolution of the different effects driving plant interactions in metalliferous ecosystems in a climate change context
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Males, Jamie Oliver. "Structure-function relationships in the water-use strategies and ecological diversity of the Bromeliaceae." Thesis, University of Cambridge, 2017. https://www.repository.cam.ac.uk/handle/1810/267920.
Анотація:
The Bromeliaceae is one of the largest and most ecologically diverse angiosperm families in the Neotropics. In recent years, this family has begun to emerge as a model system for the study of plant evolutionary ecology and physiology, and major advances have been made in understanding the factors involved in episodes of rapid diversification and adaptive radiation in specific bromeliad lineages. However, despite a long tradition of ecophysiological research on the Bromeliaceae, an integrative, evolutionarily-contextualised synthesis of the links between anatomical) physiological, and ecological aspects of bromeliad biology has hitherto been lacking. The overarching aim of this research project was therefore to use new quantitative data representing a wide range of bromeliad taxonomic and functional groups to elucidate how variation in leaf traits connected by structure-function relationships influences ecological differentiation among bromeliad taxa. Special emphasis was placed on hydraulic and water relations traits because of fast-paced contemporary developments in these fields. The methodologies employed included an assessment of the diversity of bromeliad hydrological habitat occupancy, quantification of key anatomical and physiological traits and their correlations, investigation of the links between vascular and extra-xylary anatomy and hydraulic efficiency and vulnerability, quantification of stomatal sensitivity to leaf-air vapour pressure deficit and stomatal kinetics, and a case study of trait-mediated niche segregation among congeneric epiphytic bromeliad species on the Caribbean island of Trinidad. The results highlight how divergences in a range of continuous and categorical anatomical traits underpin differences in physiological capacities and sensitivities, which in turn determine environmental relations and ecological distinctiveness. This research project therefore provides critical insights into the mechanistic basis of evolutionary diversification in a highly ecologically important family. It also represents the most comprehensive analysis of the significance of trait variation for ecological differentiation across any major radiation of herbaceous angiosperms.
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a, Daryuby Ospina Sonia Daryuby Ospina. "Linking plant strategies and ecosystem function : an assessment of the contribution of biodiversity to neotropical grassland productivity." Thesis, Bangor University, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.534448.
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ZANZOTTERA, MAGDA. "TRAIT-BASED FUNCTIONAL CHARACTERIZATION OF PLANT SPECIES AND COMMUNITIES: TRENDS AND ADAPTIONS TO ENVIRONMENT IN ALPINE AND EUROPEAN VEGETATION." Doctoral thesis, Università degli Studi di Milano, 2021. http://hdl.handle.net/2434/844440.
Анотація:
Over the last few decades, plant functional traits (morphological, physiological or phenological features, measurable at the individual level, with a direct or indirect effect on whole-plant fitness) have become widely applied to plant ecology; they provide many insights into plant responses to ecological factors, plant resource acquisition and use, plant assemblages and relations within and between communities, from individual to ecosystem scale. A small set of traits that co-vary recurrently among environments has been used to globally identify certain axes of variation, representing the different plant strategies of resources acquisition and use. In particular, plant species have been classified within a three-way strategy model (CSR) proposed by Grime, according to the distinct trait combinations the are selected under conditions of competition (C), abiotic limitation to growth (S) and periodic biomass destruction (R). Variation in plant functioning (captured by variation in trait values) and species distribution depend on variation in environmental factors that can change gradually through space and time identifying gradients which can be determined by different variables such as altitude, latitude, successional stage, resource availability, soil composition, disturbance events (grazing, fire, trampling, floods etc.) and many others.
In this study, plant functional traits and Grime’s CSR adaptive plant strategies were applied in order to gain a functional characterization of plant communities of European vegetation at different levels (within and between communities), to highlight functional similarities and/or differences and to identify common patterns and responses to environmental factors.
In details, was investigated the role of an additional trait associated to leaf nutrient content (i.e. leaf sulfur content) within the framework of both global spectrum of plant form and function and the CSR plant adaptive strategies, which resulted to be a consistent addition to the acknowledged and commonly used trait set as it was related to other traits identifying the leaf economics spectrum. Plant inter-specific trait variation in response to changes in single ecological factors was also assessed by considering gradients of different Ecological Indicators referring to key environmental drivers, demonstrating that temperature, light conditions and nutrients were associated with clear effects on plant traits, underlining that responses to changes in land use and increased soil nutrient loading could trigger and strengthen responses to climate alteration. CSR plant strategies were also applied to investigate alien species success and adaption to different habitats, showing that alien species occupy the same CSR space and, therefore, the same niches of native species, being also particularly competitive and associated to relatively productive habitats which are highly prone to invasion.
Plant functional traits variation was then studied at the community level considering a topographic sequence in in an alpine pasture, in relation to a variety of abiotic and biotic factors, which showed a strong relationship between vegetation, soil properties, topography, and grazing supporting the ability of plant strategy variation to reflect ecological parameters. In alpine environment, multiple plant communities (floristically and ecologically defined, corresponding to Habitat of EU Commmunity interest) were also functionally characterized along a successional gradient at a regional scale, finding a clear pattern of community-level trait variation that reflected the plant economics spectrum (from acquisitive and fast-growing characteristics in pioneer succession stages, to conservative and stress-tolerant features toward the succession climax) demonstrating that plant trait trade-offs can undergo adaptation at the regional scale caused by local environmental conditions. Finally, the functional characterization of classes of European vegetation using their woody diagnostic species was applied to investigate whether traits and CSR strategies reliably indicate plant-environment relations underpinned by the phytosociological classification method, suggesting a solid link between the two main approaches to vegetation description and classification, phytosociology and functional ecology.
This Ph.D. project allowed an overall better insight into plant ecology and functioning, starting from the species-level and then moving to the community-level, analysing plant adaptive strategies and trait interactions, and identifying trends and responses to environmental factors.
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Yoshikawa, Tetsuro. "Interactions between fleshy-fruited plants and avian fruit-consumers with different feeding strategies and ecological functions." Kyoto University, 2011. http://hdl.handle.net/2433/142315.
Анотація:
Kyoto University (京都大学)0048
新制・課程博士
博士(農学)
甲第16117号
農博第1853号
新制||農||988(附属図書館)
学位論文||H23||N4587(農学部図書室)
28696
京都大学大学院農学研究科森林科学専攻
(主査)教授 井鷺 裕司, 教授 太田 誠一, 教授 大澤 晃
学位規則第4条第1項該当
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Lane, Robin. "Evaluation of a standardized protocol for parent training in positive behavior support using a multiple baseline design." [Tampa, Fla] : University of South Florida, 2008. http://purl.fcla.edu/usf/dc/et/SFE0002707.
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Vincenzi, Leonardo Braga de. "Gestão social no planejamento urbano?: o caso da cidade do Rio de Janeiro." reponame:Repositório Institucional do FGV, 2008. http://hdl.handle.net/10438/16977.
Анотація:
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Paralelamente ao surgimento da gestão estratégica, emerge no cenário nacional um novo enfoque administrativo: a gestão social. E o planejamento urbano é uma das áreas da administração pública que mais pode beneficiar-se desse modelo de tomada de decisão. A Lei do Estatuto da Cidade estabelece um conjunto de regras para a ordenação do uso do solo urbano, e obriga os municípios com mais de 20 mil habitantes a apresentar um Plano Diretor. Ela traz, entre outras novidades legislativas, a gestão social e a função social da propriedade, repercutindo sob aspectos como, dentre outros, o econômico, o político, o social e o ambiental. O Brasil – até há poucas décadas basicamente um país agrário – passou por um acelerado processo de urbanização, com a migração de trabalhadores oriundos do campo. Até então, o planejamento urbano era feito por uma estrutura burocrática centralizada, autoritária, e com interesses sobretudo especulativos e econômicos. Em decorrência, ocorreram um crescimento desordenado da população de baixa renda na área urbana, bem como uma acentuada proliferação de atividades urbanas informais. O presente trabalho analisa, brevemente e de forma geral, como a implementação da gestão social pelo Estatuto da Cidade e o Plano Diretor alteram essa realidade, e como ela pode influenciar o desenvolvimento e o planejamento urbano carioca.
In parallel to strategic management, a new administrative approach emerges in the national scene: the social management. Urban planning is one of the areas of the public administration that can best be benefited from this model of decision-making. The City Statute Law establishes a set of rules aiming at urban land utilization. It makes compulsory the presentation of a Director Plan by all municipalities with more than twenty thousand inhabitants. This brings, among other legislative innovations, social management and the social function of property, with repercussions in several aspects such as, among others, economic, political, social and environmental conditions. Brazil – basically an agrarian country up to a few decades ago – went through a fast urbanization process due to workers migration from rural areas. The urban planning used to be carried out by a bureaucratic, centralized, authoritative structure, mainly with speculative and economic interests. As a result, a disorderedly low income population growth came up to urban areas with striking proliferation of informal urban activities. This paper analyses briefly and in a general view how implementation of social management through the City Statute Law and the Director Plan is changing this situation and how it may influence urban development and planning in Rio de Janeiro City.
Книги з теми "Plant functional strategies":
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Mertens, Elke. Visualizing landscape architecture: Functions, concepts, strategies. Basel: Birkhäuser, 2010.
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Cevelev, Aleksandr. Strategic development of railway transport logistics. ru: INFRA-M Academic Publishing LLC., 2021. http://dx.doi.org/10.12737/1194747.
Анотація:
The monograph is devoted to the methodology of material and technical support of railway transport. According to the types of activities, the nature of the material and technical resources used, technologies, means and management systems, Russian railways belong to the category of high-tech industries that must have high quality and technical level, reliability and technological efficiency in operation. For this reason, the logistics system itself, both in structure and in the algorithm of the functions performed as a whole, needs a serious improvement in the quality of its work. The economic situation in Russia requires a revision of the principles and mechanisms of management based on the corporate model of supply chain management, focused on logistics knowledge. In the difficult economic conditions of the current decade, it is necessary to improve the quality of the supply organization of enterprises and structural divisions of railway transport, directly related to the implementation of the process approach, the advantage of which is a more detailed regulation of management actions and their mutual coordination. In order to increase the efficiency of its activities and develop the management system, Russian Railways is developing a lean production system aimed at further expanding the implementation of the principles of customer orientation, ideology and corporate culture.
At the present time, the solution of many issues is impossible without a cybernetic approach to the formulation of problems of material and technical support and logistics analysis of information technologies, to the implementation of the developed algorithms and models of development strategies and concepts for improving the business processes of the production system. The management strategy, or the general plan for the implementation of activities for the management of material resources, is based on a fundamental assessment of the alignment and correlation of forces and factors operating in the economic and political field, taking into account the impact on the specific form of the management strategy.
The materials will be useful to the heads and specialists of the directorates of the MTO, CDZs and can be used in the scientific research of bachelors, masters and postgraduates interested in the economics of railway transport and supply logistics.
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Taberlet, Pierre, Aurélie Bonin, Lucie Zinger, and Eric Coissac. Environmental DNA for functional diversity. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198767220.003.0010.
Анотація:
Chapter 10 “Environmental DNA for functional diversity” discusses the potential of environmental DNA to assess functional diversity. It first focuses on DNA metabarcoding and discusses the extent to which this approach can be used and/or optimized to retrieve meaningful information on the functions of the target community. This knowledge usually involves coarsely defined functional groups (e.g., woody, leguminous, graminoid plants; shredders or decomposer soil organisms; pathogenicity or decomposition role of certain microorganisms). Chapter 10 then introduces metagenomics and metatranscriptomics approaches, their advantages, but also the challenges and solutions to appropriately sampling, sequencing these complex DNA/RNA populations. Chapter 10 finally presents several strategies and software to analyze metagenomes/metatranscriptomes, and discusses their pros and cons.
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Long, Sebastian. Designing a Games User Research lab from scratch. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198794844.003.0006.
Анотація:
Towards informing the development of new playtesting labs, this chapter outlines the process we used at Player Research for setting up our labs. Setting up a functional lab environment requires a range of considerations—across floor plans, materials selection, technology choices, testing strategies, and more—and in this chapter we describe some of the key lessons we learned along the way.
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Rengel, Roberto J. The Interior Plan. 3rd ed. Bloomsbury Publishing Inc, 2023. http://dx.doi.org/10.5040/9781501369681.
Анотація:
The Interior Plan is an introductory-level text introducing students to the process of interior design space planning. Topics include the design of effective spatial sequences, functional relationships among project parts, arrangement of furniture, planning effective circulation systems, making spaces accessible, and designing safe environments with efficient emergency egress systems. Exercises throughout the book facilitate learning by encouraging students to apply ideas and concepts immediately after reading about them. In the third edition, the author expands on the evolution of design ideas and how they affect interior environments and the people who use them, thinking sustainably, and how interior elevations and reflective ceilings affect the plan. New to This Edition -New and updated examples in residential, commercial, retail, and hospitality planning -New spreads addressing interior elevations, reflected ceiling plans, and interior planning for sustainability -New pedagogical features in every chapter, including learning objectives, discussion questions, and key terms Instructor Resources -Instructor’s Guide provides suggestions for planning the course and using the text in the classroom, supplemental assignments, and lecture notes -PowerPoint® presentations include images from the book and provide a framework for lecture and discussion STUDIO Features Include: -Download examples of common design pitfalls and how to avoid them -Review concepts with flashcards of essential vocabulary and design strategies
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Fullner, Sheryl Kindle. The Shoestring Library. ABC-CLIO, LLC, 2010. http://dx.doi.org/10.5040/9798216014430.
Анотація:
An organized collection of budget saving methods, materials, and strategies, these tips are all tried-and-true examples of ways to stretch the media specialist's budget and time, and change even the drabbest library into an inviting oasis of learning. The Shoestring Library offers hope, incentive, and direction to librarians who lack everything but passion. The book is organized around 300 hints—more than 114 of which are green alternatives—for administering a library in tough times. The book is divided into two parts, Support Functions and Physical Plant. The support section investigates such topics as how to best use volunteers and how to get free materials for your library. The physical plant section, amply supplemented with dozens of photos, helps a librarian identify and fix problems with dismal walls, ceilings, windows, shelving, desks, seating, and lighting, proposing low-cost or no-cost solutions to all these woes. Instructions are practical and thorough, concentrating on reuse, re-purposing, and recycling, and each idea is reversible. For quick access, chapters sport distinctive icons; time-management tips, for example, are distinguished by an alarm clock. Designed for institutions from preschools through college, The Shoestring Library proves that there is no expiration date on learning.
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Matthews, Joseph R. Research-Based Planning for Public Libraries. ABC-CLIO, LLC, 2013. http://dx.doi.org/10.5040/9798216007890.
Анотація:
This book will help public library administrators, managers, and board members to better plan, strategize, and understand their communities, enabling public libraries to become dynamic, proactive institutions. Research-Based Planning for Public Libraries: Increasing Relevance in the Digital Age takes readers through a logical and effective process for developing a plan and implementing it within the various functions of the library. Grounded in research and best practices, the book offers practical, easy-to-implement advice and direction for today’s public library administrators, managers, and board members. Covering everything from goal-setting, policy-making, and budgeting, to collections, promotions, and access and evaluation, the book details how to better provide and promote access, convey its value to customers, and make the library a more integral part of the community. The author inspires library staff and administrators to reinvent themselves to meet�and overcome�the current challenges they face. The information is specifically tailored towards public librarians, particularly those in management or administration, as well as to LIS faculty and students of public librarianship and library management.
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Roe, Simon, ed. Protein Purification Techniques. Oxford University Press, 2001. http://dx.doi.org/10.1093/oso/9780199636747.001.0001.
Анотація:
Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set)are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.
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Yang, Jingduan, and Daniel A. Monti. Clinical Acupuncture and Ancient Chinese Medicine. Oxford University Press, 2017. http://dx.doi.org/10.1093/med/9780190210052.001.0001.
Анотація:
Clinical Acupuncture and Ancient Chinese Medicine provides health care professionals interested in learning or practicing acupuncture the essential theoretical foundation of Chinese medicine on which an effective acupuncture therapy must be based. It describes in detail the human energetic anatomy, physiology, pathophysiology, and etiology for both mental and physical functions of children, men, women, and the elderly. It offers a step-by-step algorithm for diagnosing physical or mental ailments with diagnostic techniques and formulation processes and treating them with effective strategies, plans, and acupuncture techniques. Clinical Acupuncture and Ancient Chinese Medicine also presents acupuncture as an energy medicine, in contrast to modern medicine, which is a more biochemically and structurally based medicine. Both are integral parts in the spectrum of human medicine, more complementary than alternative to each other. This book helps readers to study and practice acupuncture as part of their continued medical education (CME) and as a natural expansion of their practice to provide additional care for their patients at the energetic level at which a majority of ailments lay. The content is organized in a way that parallels modern medicine so readers can more easily relate and understand concepts that may be otherwise foreign to them. This book describes human health with the belief that a human being is essentially an energetic being and that the interaction of human energy with the energy of nature and the universe is critical to maintaining a healthy life. It provides useful contents for self-care and the ancient practice of life cultivation.
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Lederman, Gordon. Reorganizing the Joint Chiefs of Staff. Greenwood Publishing Group, Inc., 1999. http://dx.doi.org/10.5040/9798216007630.
Анотація:
The Goldwater-Nichols Department of Defense Reorganization Act of 1986 is the most important legislation to affecting U.S. national defense in the last 50 years. This act resulted from frustration in Congress and among certain military officers concerning what they believed to be the poor quality of military advice available to civilian decision-makers. It also derived from the U.S. military's perceived inability to conduct successful joint or multi-service operations. The act, passes after four years of legislative debate, designated the Chairman of the Joint Chiefs of Staff as the principal military advisor to the President and sought to foster greater cooperation among the military services. Goldwater-Nichols marks the latest attempt to balance competing tendencies within the Department of Defense, namely centralization versus decentralization and geographic versus functional distributions of power. As a result of the Goldwater-Nichols Act, the Chairman of the Joint Chiefs has achieved prominence, but his assignment is somewhat contradictory: the spokesman and thus the advocate for the Commander in Chief, while simultaneously the provider of objective advice to the President. While the act did succeed in strengthening the CINCs' authority and in contributing to the dramatic U.S. achievements in the Gulf War, the air and ground campaigns revealed weaknesses in the CINCs' capability to plan joint operations. In addition, the increased role of the military in ad hoc peacekeeping operations has challenged the U.S. military's current organizational structure for the quick deployment of troops from the various services. Rapid technological advances and post-Cold War strategic uncertainty also complicate the U.S. military's organizational structure.
Частини книг з теми "Plant functional strategies":
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Gutschick, Vincent P. "Functional Biology and Plant Strategies." In A Functional Biology of Crop Plants, 1–36. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4615-9801-5_1.
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Hurrah, Ishfaq Majid, Tabasum Mohiuddin, Sayanti Mandal, Mimosa Ghorai, Sayan Bhattacharya, Potshangbam Nongdam, Abdel Rahman Al-Tawaha, et al. "Strategies and Applications of Genomic Editing in Plants with CRISPR/Cas9." In CRISPR and Plant Functional Genomics, 59–80. Boca Raton: CRC Press, 2024. http://dx.doi.org/10.1201/9781003387060-4.
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Sen, Priyabrata, Charu Lata, Kanti Kiran, and Tapan Kumar Mondal. "RNA Interference (RNAi) in Functional Genomics of Wheat." In Concepts and Strategies in Plant Sciences, 239–64. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-63372-1_10.
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Khan, Mohammad Saghir, Almas Zaidi, P. A. Wani, Munees Ahemad, and Mohammad Oves. "Functional Diversity Among Plant Growth-Promoting Rhizobacteria: Current Status." In Microbial Strategies for Crop Improvement, 105–32. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-642-01979-1_6.
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Singh, Ram Kushal, and Sujeet Pratap Singh. "Prospects of Functional Genomics in Sugarcane." In Advances in Plant Breeding Strategies: Breeding, Biotechnology and Molecular Tools, 489–511. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-22521-0_17.
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Kumar, Vinay, Lata Jain, Swati Jain, and Ravindra Soni. "Microbe-Mediated Plant Functional Traits and Stress Tolerance: The Multi-Omics Approaches." In Survival Strategies in Cold-adapted Microorganisms, 233–51. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-2625-8_11.
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Anai, Toyoaki. "Mutant-Based Reverse Genetics for Functional Genomics of Non-model Crops." In Advances in Plant Breeding Strategies: Breeding, Biotechnology and Molecular Tools, 473–87. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-22521-0_16.
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Brown, Gary, and Gary R. Feulner. "The Vascular Flora of the United Arab Emirates." In A Natural History of the Emirates, 387–425. Cham: Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-37397-8_13.
Анотація:
AbstractThis chapter discusses the flora of the United Arab Emirates (UAE), focusing on various aspects of the individual plant species. A brief overview of salient features of the flora is given in terms of species number and families, followed by a short discussion regarding some of the taxonomic problems surrounding the naming and identification of species. With respect to biogeographical aspects, it is emphasised that the current flora of the nation represents a distinct snapshot in time that has been shaped by a diversity of events in the past, all of which continue to operate on different spatio-temporal scales. After a brief discussion of keystone and foundation species in the UAE desert, autecological aspects are examined, as these are fundamental to understanding the response of plants to a changing environment. Following on from this topic, the concepts of life forms, plant functional groups and plant strategies are touched upon. In the final section, some remarkable features of the reproductive biology of desert plants are described. Chapter 5 examines the typical plant communities and also looks at the main threats to the flora and vegetation of the country.
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Guo, Hui-jun, Yong-dun Xie, Lin-shu Zhao, Hong-chun Xiong, Jia-yu Gu, Shi-rong Zhao, and Lu-xiang Liu. "Progress of mutant resource development and tilling on starch biosynthesis in wheat." In Mutation breeding, genetic diversity and crop adaptation to climate change, 280–84. Wallingford: CABI, 2021. http://dx.doi.org/10.1079/9781789249095.0028.
Анотація:
Abstract Induced mutations have been widely utilized for the development of plant mutant germplasm and varieties since 1927 and have contributed to genetic diversity enhancement and food security in the world. Mutant resources are essential for gene identification and functional characterization by forward and reverse genetic strategies. The publishing of annotated wheat reference genomes is greatly promoting the progress of wheat functional genomic research. Mutant resources of a broad spectrum and diversified wild- types will be the prerequisites in this process, in part due to the polyploid nature of wheat. This review describes the progress of mutant resource development derived from the winter wheat cultivar 'Jing411'. The segregating M2 population has been used for mining functional mutant alleles of key genes involved in starch biosynthesis and could be further used for allele mining of any other target genes. The morphological mutant resources developed from various mutagens have been, and are going to be, used to develop genetic populations for gene mapping and the genetic analysis of biological functions.
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Schimel, David, Philip A. Townsend, and Ryan Pavlick. "Prospects and Pitfalls for Spectroscopic Remote Sensing of Biodiversity at the Global Scale." In Remote Sensing of Plant Biodiversity, 503–18. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-33157-3_19.
Анотація:
AbstractImaging spectroscopy is a powerful new approach for observing aspects of the biological diversity of the Earth, conveying information about plant functional traits, habitat, and plant diversity itself. For decades, spectroscopic data suitable for this application have mainly been collected by aircraft. But in the next decade, global coverage from space by high-quality spectroscopic data will become available, preceded by instruments providing “global access”—not wall-to-wall coverage but data from almost anywhere in the world. For decades, scientists have experimented with and discussed optimal strategies for collecting spectroscopic data, but the next set of missions is now sufficiently well-defined that ecologists should consider how best to use the data that can now be expected. The anticipated flood of data will provide a new window on diversity, characterizing it in new ways that comprehensively sample space and change over time. Spectroscopic data will be peta-scale or larger, perhaps as much as 10 TB per day, and the data themselves will be high dimensional, requiring and allowing advanced big data techniques to be fully exploited. These data raise specific challenges such as how to characterize aggregate ecosystem characteristics, since the traits observed will change with phenology. Pixels will be fixed at ~30 m, 10–106 times larger than the plants they sample; other instrument objectives are likely in the range of 10 nm spectral sampling, coverage from 400 to 2500 nm with signal to noise in the range of 250–400. Imaging spectroscopy from space represents a huge opportunity for global ecology, but many conceptual, algorithmic, and theoretical issues will challenge the users.
Тези доповідей конференцій з теми "Plant functional strategies":
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Cetiner, Sacit M., David L. Fugate, Roger A. Kisner, Michael D. Muhlheim, and Richard T. Wood. "Development of a Supervisory Control System Concept for Advanced Small Modular Reactors." In ASME 2014 Small Modular Reactors Symposium. American Society of Mechanical Engineers, 2014. http://dx.doi.org/10.1115/smr2014-3403.
Анотація:
Small modular reactors (SMRs) can provide the United States with a safe, sustainable, and carbon-neutral energy source. Because of their small size and, in many cases, simplified nuclear island configurations, it is expected that capital costs will be less for SMRs compared to that of large, Generation III+ light-water reactors. Advanced SMRs (AdvSMRs), which use coolants other than water as the primary heat transport medium, introduce several passive safety concepts and controls features that further reduce the complexity of primary system designs by eliminating redundant components and systems. Under U.S. Department of Energy (DOE) Office of Nuclear Energy (NE), the Supervisory Control of Multi-Modular SMR Plants project was established to enable innovative control strategies and methods to supervise multi-unit plants, accommodate shared systems, identify opportunities to increase the level of automation, define economic metrics based on the relationship between control and staffing levels, and permit flexible co-generation operational regimes. This paper documents current findings from the Supervisory Control project. Specifically, it defines and documents strategies, functional elements, and an architectural structure for supervisory control of a representative generic AdvSMR plant. More specifically, this research advances the state-of-the art by incorporating decision making into the supervisory control system architectural layers through the introduction of tiered taxonomy of plant systems and subsystems. The proposed architecture has the features of planning and scheduling, analyzing plant status, diagnosing problems as they develop and predicting potential future problems, making decisions based on these features, and generating validated commands to lower control layers in the architecture.
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Dias, Fernanda Furlan Goncalves, Juliana M. Leite Nobrega De Moura Bell, and Kazunori Machida. "Bioprocessing strategies to improve the extractability and functional properties of lipids, proteins, and carbohydrates from full-fat chickpea flour." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/tajg6981.
Анотація:
Interest in sustainable cost-effective and efficient extraction processes of plant-based matrices has risen due to health, environmental, and social concerns. The concurrent extraction of lipids, proteins, and carbohydrates can be achieved by aqueous and enzymatic extraction processes, addressing low extractability by mechanical pressing and the use of flammable solvents. Being a source of proteins, starch, and fiber, chickpeas can be used as a matrix to generate added value compounds for food, feed, and fuel applications. The application of alkaline protease, preceded or not by carbohydrase pretreatments, was evaluated on the extractability of oil, protein, and carbohydrates from full-fat chickpea flour and protein functionality. Enzymatic extraction increased oil and protein extractability from 49.8 to 72.0–77.1% and 62.8 to 83.5–86.1%, respectively. Although the use of carbohydrase pretreatments, before the addition of protease, did not increase oil and protein extractability, it did increase the carbohydrate content of the extracts from 7.7 to 9.2–9.3 mg/mL, with the release of new oligosaccharides revealed by LC-MS/MS. Enzymatic extraction produced proteins with higher solubility (25.6 vs. 68.2–73.6%) and in vitro protein digestibility (83.8 vs. 90.79–94.7%). Post-extraction -galactosidase treatment of the extracts completely hydrolyzed flatulence-causing oligosaccharides into simple sugars. However, because high water usage is a requirement to maximize extractability in single-stage extractions, a two-stage countercurrent extraction process was developed to reduce water usage without loss in extractability. Countercurrent extraction reduced ~50% of the water used in the extraction while increasing oil and protein extractability to 95.8 and 95.7%, respectively. Techno-economic analysis demonstrated improved annual profitability of the countercurrent process despite its higher operating costs. This study highlights the effectiveness of solvent-free enzymatic strategies to maximize process extractability and feasibility, shedding light on the development of a more competitive process to produce chickpea proteins, lipids, and potential prebiotic oligosaccharides for subsequent industrial applications.
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Stroia, Ciprian, Madalina Corcan, Ionel Samfira, Marius Stroia, and Lucretiu Dancea. "THE BIODIVERSITY OF THE GRASSLANDS IN WESTERN ROMANIA FOR THEIR SUSTAINABLE." In 22nd SGEM International Multidisciplinary Scientific GeoConference 2022. STEF92 Technology, 2022. http://dx.doi.org/10.5593/sgem2022v/6.2/s25.42.
Анотація:
The productivity of the grasslands is determined by the biodiversity of the plant species present in the vegetation, their quality, but also their exploitation. For their inventory, a series of determination methods are used, methods that are difficult to manage and that require a good knowledge of the plant species. For an agronomic characterization of the plots, it is sufficient to consider only the main species that dominate the plant biomass. A more simplified method is that of botanical surveys which focus only on recognizing the dominant grasses in grasslands. This method allows for an agronomic diagnosis of a grassland without modifying the variables studied, namely, the percentage of biomass participation and the percentage of each functional type of grasses. The method allows the application of a functional typology that can estimate the value of use (forage quality, productivity, precocity or lateness) of a grassland. The purpose of this work is to classify the species by plant families, establish the pastoral value and inventory the grass species, as well as classify them from a morphofunctional point of view, in order to implement some management strategies aimed at ensuring an optimal exploitation of the grasslands taken into study. The measurements were made on three grasslands belonging to the town of Sasca Montana, Romania, located in the south of the Western Carpathians (44�53'13"N 21�42'33"E), at an altitude of 237 m. In this area, the average annual temperatures is between 9 - 10�C, and the precipitation level is between 700 and 800 mm. The three studied grasslands have a similar floristic composition, but with a different participation percentage of species. The dominant grass species, from a morpho-functional point of view, are species of type C (Festuca valesiaca being the dominant species), which shows that these grasslands are weak and do not adapt to mowing practices, although the species included in this morpho-functional type are species with a fairly good fodder value in the vegetative phase.
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Bushart, Sean, Karen Kim, and Michael Naughton. "Program Change Management During Nuclear Power Plant Decommissioning." In ASME 2010 13th International Conference on Environmental Remediation and Radioactive Waste Management. ASMEDC, 2010. http://dx.doi.org/10.1115/icem2010-40129.
Анотація:
Decommissioning a nuclear power plant is a complex project. The project involves the coordination of several different departments and the management of changing plant conditions, programs, and regulations. As certain project Milestones are met, the evolution of such plant programs and regulations can help optimize project execution and cost. This paper will provide information about these Milestones and the plant departments and programs that change throughout a decommissioning project. The initial challenge in the decommissioning of a nuclear plant is the development of a definitive plan for such a complex project. EPRI has published several reports related to decommissioning planning. These earlier reports provided general guidance in formulating a Decommissioning Plan. This Change Management paper will draw from the experience gained in the last decade in decommissioning of nuclear plants. The paper discusses decommissioning in terms of a sequence of major Milestones. The plant programs, associated plans and actions, and staffing are discussed based upon experiences from the following power reactor facilities: Maine Yankee Atomic Power Plant, Yankee Nuclear Power Station, and the Haddam Neck Plant. Significant lessons learned from other sites are also discussed as appropriate. Planning is a crucial ingredient of successful decommissioning projects. The development of a definitive Decommissioning Plan can result in considerable project savings. The decommissioning plants in the U.S. have planned and executed their projects using different strategies based on their unique plant circumstances. However, experience has shown that similar project milestones and actions applied through all of these projects. This allows each plant to learn from the experiences of the preceding projects. As the plant transitions from an operating plant through decommissioning, the reduction and termination of defunct programs and regulations can help optimize all facets of decommissioning. This information, learned through trial in previous plants, can be incorporated into the decommissioning plan of future projects so that the benefits of optimization can be realized from the beginning of the projects. This process of the collection of information and lessons learned from plant experiences is an important function of the EPRI Decommissioning Program.
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Prabhu, Padmanabha J., and Damian A. Testa. "Steam Generator Asset Management Program." In 18th International Conference on Nuclear Engineering. ASMEDC, 2010. http://dx.doi.org/10.1115/icone18-29471.
Анотація:
The Steam Generator Asset Management Program (SGAMP) is a long term program designed to maximize the performance and reliability of the steam generators. The SGAMP focuses on plant specific conditions and hence is applicable to the original or the replacement steam generators. It is recommended that the utility and the vendor form a joint steam generator management team (SGMT) to develop, monitor and implement a long-term plan to address steam generator operation, maintenance and life extension goals. The SGMT will consist of representatives from operations, chemistry, maintenance and engineering functions and will be responsible for making decisions related to the steam generators. The charter of the SGMT is to develop a steam generator strategic plan that will cost-effectively manage steam generator options. The strategic plan is consistent with the Steam Generator Program Guidelines (NEI 97-06 in the United States). The strategic plan is a living document and is revised periodically to incorporate inspection results, new technology developments, lessons learned and industry experience. Cost-benefit analyses of strategies may be performed to prolong steam generator operability through steam generator performance modeling (tube degradation, fouling, etc.), diagnostic tools, regulatory strategy, condition monitoring and operational assessment strategy, and maintenance strategy. The SGMT will provide input regarding potential maintenance of the steam generators with schedule and cost impacts for each outage. It will also recommend engineering evaluations to be performed in support of program goals and will develop short- and long-term recommendations. These recommendations will address action plans, performance measures and results. Secondary side inspection and cleaning strategy should be developed (techniques and frequency) to maximize performance cost-effectively. This paper is based on Westinghouse experience gained by working with several pressurized water reactor (PWR) plant operators in the United States (US).
6
Ai, Yongfeng. "Pulse starch as a promising gelling agent and resistant starch source for industrial applications." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/gkpg9582.
Анотація:
As sustainable and protein-rich crops, pulses—including pea, lentil, faba bean, and dry bean—have attracted tremendous interests for the production of plant-based proteins. Nevertheless, starch is the main constituent of pulse grains, the content of which typically ranges from 35% to 52%. Therefore, the global pulse processing industry is developing effective strategies to find new markets for this co-product from pulse protein fractionation. Our recent research demonstrated that: (1) under normal processing conditions (e.g., at 95 °C cooking), round pea and lentil starches formed remarkably stronger gels than commercial starches from other botanical sources; (2) under high-temperature processing conditions (e.g., at 140 °C cooking), wrinkled pea starch developed the firmest gel among common native starches. The gelation mechanisms of various pulse starches over cooking temperatures of 95–140 °C will be illustrated in the presentation. Having relatively higher amylose contents (35–80%) than common commercial starches, pulse starches can be more suitable for the development of resistant starch (RS), a new type of dietary fiber that can deliver low glycemic and insulinemic benefits to consumers. Heat-moisture treatment (HMT) effectively increased the RS contents of round pea (CDC Bronco variety) and wrinkled pea (MPG87 variety) starches from 5.8% to 11.0% and from 21.4% to 29.9%, respectively. The “structure-function-digestibility” relationships of the HMT-modified pea starches will be discussed. The presented unique functional and nutritional attributes of pulse starches will be meaningful for the agri-food industry to generate new value from this leading carbohydrate component in pulse seeds.
7
Oboimov, Anton, Oleg Zolotarev, Ivan Cheremisenov, E. Fedoseeva, A. Tokarev, E. Razina, and A. Galieva. "Assessing the risk of a nuclear power plant in a terrorist attack. Application of physical protection systems. Ray-tracing technology optimization techniques." In International Conference "Computing for Physics and Technology - CPT2020". ANO «Scientific and Research Center for Information in Physics and Technique», 2020. http://dx.doi.org/10.30987/conferencearticle_5fd755c05b7c88.92154155.
Анотація:
Terrorism today is one of the main threats to human civilization. In recent years, international terror has proved its extreme cruelty, its readiness to stop at nothing in order to achieve its goals. As a result of committing terrorist acts, innocent people, children, women and old people suffer. For a terrorist, human life has no value. With the modern variety of potentially dangerous objects and their functional orientation, it is impossible to ensure safety with standard solutions, using uniform strategies, tactical schemes and techniques, and the same type of equipment. If we simply follow the path of ensuring the maximum level of safety, it may turn out that at most facilities the cost of the applied technological means will be many times greater than the cost of the technological equipment of the facility and even the facility itself. Hence, it follows that in order to create effective physical protection systems or to effectively modernize existing physical protection systems, it is necessary to analyze these systems, to identify the weakest and most protected places that need strengthening. At the same time, an important difference must be taken into account between physical protection systems and other safety systems used to protect against natural disasters (such as earthquakes, floods, tornadoes, hurricanes, etc.) and against abnormal operating conditions (such as fire, malfunctions in electrical equipment, etc.). Unlike human violators, security breaches are subject to strict laws and do not happen without reason. For example, the fire of a fire can only burn while there is fuel and oxygen. If one of these elements is removed, the fire will go out. Also, a fire cannot decide where and when it will occur, and how it will progress. Conversely, the human adversary (intruder) has the ability to decide whether to attack, when and how to attack, can adjust to the measures of the physical protection system to counter it, and possibly even outwit and defeat them. All this introduces a significant element of uncertainty into the process of analyzing and evaluating the effectiveness of the physical protection system. Taking into account the above, the task of developing a methodological approach to risk assessment when committing a terrorist attack using physical protection systems, taking into account the optimization of calculations of cost-intensive algorithms in real time, becomes more and more urgent. In this article, the authors attempt to create this approach.
8
Akbar, Sajjad. "Industrial Maintenance Strategies." In 14th International Conference on Nuclear Engineering. ASMEDC, 2006. http://dx.doi.org/10.1115/icone14-89148.
Анотація:
Industrial plants have become more complex due to technological advancement. This has made the task of maintenance more difficult. The maintenance costs in terms of resources and downtime loss are so high that maintenance function has become a critical factor in a plant’s profitability. Industry should devote as much forethought to the management of maintenance function as to production. Maintenance has grown from an art to a precise, technical engineering science. Planning, organizing scheduling and control of maintenance using modern techniques pays dividends in the form of reduced costs and increased reliability. The magnitude and the dimension of maintenance have multiplied due to development in the engineering technologies. Production cost and capacities are directly affected by the breakdown time. Total operating cost including the maintenance cost plays an important role in replacement dimension. The integrated system approach would bring forth the desired results of high maintenance standards. The standards once achieved and sustained, would add to the reliability of the plan and relieve heavy stresses and strains on the engineering logistic support.
9
Nopper, Helmut, Roland Ro¨ßner, and Andre´ Zander. "Aging and Plant Life Management with the Software Tool COMSY." In 14th International Conference on Nuclear Engineering. ASMEDC, 2006. http://dx.doi.org/10.1115/icone14-89597.
Анотація:
Within the scope of PLEX, a systematic and efficient ageing and plant life management system is becoming more and more important to ensure a safe and economical power plant operation in spite of continuous plant ageing. For the methodical implementation of PLIM & PLEX strategies, AREVA NP has developed the software tool COMSY. This knowledge-based program integrates degradation analysis tools with an inspection data management system. COMSY provides the capability to establish a program guided technical documentation by utilizing a virtual plant model which includes information regarding thermal hydraulic operation, water chemical conditions and materials applied for mechanical components. It provides the option to perform a plant-wide screening for identifying system areas, which are sensitive for degradation mechanisms typically experienced in nuclear power plants (FAC, corrosion fatigue, IGSCC, Pitting, etc.). If a system area is identified as being susceptible to degradation, a detailed analysis function enables the condition-oriented service life evaluation of vessels and piping systems in order to localize and conservatively quantify the effect of degradation. Based on these forecasts with COMSY, specific strategies can be developed to mitigate the effect of degradation and inspection activities can be focused on degradation sensitive areas. In addition, a risk-informed assessment tool serves to optimize inspection activities in respect to degradation potential and the associated damage consequence. After an in-service inspection is performed for a distinct location, the inspection data is to be evaluated according to generally accepted procedures. For this purpose an integrated inspection data management system module provides standardized, interactively operated evaluation functions. The key inspection results are transmitted as feedback in respect to the as-is condition of the component. Subsequently, all further life evaluations of the associated component are calibrated against the inspection results. The compiled condition-oriented knowledge provides the basis for a continuous optimization resulting in tailored inspection and maintenance programs geared to the specific plant. The systematic closed loop process ensures the generation of up-to-date plant documentation relating to the technical as-is status of the plant, as all the data involved in the process are compiled in a “living” documentation structure. The implementation of COMSY in various nuclear power plants has confirmed that systematic plant life management makes good economic sense, as cost reductions can be achieved while increasing the plants availability.
10
Noman, M., J. Zafar, and A. Awase. "Base Management Production System Optimization." In PAPG/SPE Pakistan Section Annual Technical Symposium and Exhibition. SPE, 2023. http://dx.doi.org/10.2118/217368-ms.
Анотація:
ABSTRACT Base management provides framework for managing the production base and maximizing recovery through efficient depletion. A vital element of base management practice is system optimization which includes modelling, prioritizing, and implementing strategies to enhance production & reserves of all fields. The process starts with a holistic network model1 that includes integrating reservoir, wellbore hydraulics, gathering system and production facilities. System wide evaluation is conducted using this integrated model and recommendations are networked with cross-functional teams. Finally, these observations are ranked and executed as per business requirements. Given the vast number of active/shut-in wells & fundamentally different field types operated by UEP, this approach is pivotal to effectively manage base production. This study focuses on key achievements in past few years which delivered fruitful results in terms of production and reserves addition. Below is the summary of activities: Area-1 comprises of aging fields and the focus has been on creating network flexibility, re-accessing bypassed zones, and compressors modifications to bring the system in the most optimized operating window. Area-2 is primarily gas producing region and opportunities on Well hydraulics, surface compression, system de-bottlenecking, and plant optimization remain in the forefront. Figure-3 demonstrates a case of successfully managed base through multiple optimization activities. The initiatives discussed in this study significantly contributed to optimizing base production of Area-1 & 2, enabling them to maintain optimum production levels.
Звіти організацій з теми "Plant functional strategies":
1
Ron, Eliora, and Eugene Eugene Nester. Global functional genomics of plant cell transformation by agrobacterium. United States Department of Agriculture, March 2009. http://dx.doi.org/10.32747/2009.7695860.bard.
Анотація:
The aim of this study was to carry out a global functional genomics analysis of plant cell transformation by Agrobacterium in order to define and characterize the physiology of Agrobacterium in the acidic environment of a wounded plant. We planed to study the proteome and transcriptome of Agrobacterium in response to a change in pH, from 7.2 to 5.5 and identify genes and circuits directly involved in this change. Bacteria-plant interactions involve a large number of global regulatory systems, which are essential for protection against new stressful conditions. The interaction of bacteria with their hosts has been previously studied by genetic-physiological methods. We wanted to make use of the new capabilities to study these interactions on a global scale, using transcription analysis (transcriptomics, microarrays) and proteomics (2D gel electrophoresis and mass spectrometry). The results provided extensive data on the functional genomics under conditions that partially mimic plant infection and – in addition - revealed some surprising and significant data. Thus, we identified the genes whose expression is modulated when Agrobacterium is grown under the acidic conditions found in the rhizosphere (pH 5.5), an essential environmental factor in Agrobacterium – plant interactions essential for induction of the virulence program by plant signal molecules. Among the 45 genes whose expression was significantly elevated, of special interest is the two-component chromosomally encoded system, ChvG/I which is involved in regulating acid inducible genes. A second exciting system under acid and ChvG/Icontrol is a secretion system for proteins, T6SS, encoded by 14 genes which appears to be important for Rhizobium leguminosarum nodule formation and nitrogen fixation and for virulence of Agrobacterium. The proteome analysis revealed that gamma aminobutyric acid (GABA), a metabolite secreted by wounded plants, induces the synthesis of an Agrobacterium lactonase which degrades the quorum sensing signal, N-acyl homoserine lactone (AHL), resulting in attenuation of virulence. In addition, through a transcriptomic analysis of Agrobacterium growing at the pH of the rhizosphere (pH=5.5), we demonstrated that salicylic acid (SA) a well-studied plant signal molecule important in plant defense, attenuates Agrobacterium virulence in two distinct ways - by down regulating the synthesis of the virulence (vir) genes required for the processing and transfer of the T-DNA and by inducing the same lactonase, which in turn degrades the AHL. Thus, GABA and SA with different molecular structures, induce the expression of these same genes. The identification of genes whose expression is modulated by conditions that mimic plant infection, as well as the identification of regulatory molecules that help control the early stages of infection, advance our understanding of this complex bacterial-plant interaction and has immediate potential applications to modify it. We expect that the data generated by our research will be used to develop novel strategies for the control of crown gall disease. Moreover, these results will also provide the basis for future biotechnological approaches that will use genetic manipulations to improve bacterial-plant interactions, leading to more efficient DNA transfer to recalcitrant plants and robust symbiosis. These advances will, in turn, contribute to plant protection by introducing genes for resistance against other bacteria, pests and environmental stress.
2
Ostersetzer-Biran, Oren, and Jeffrey Mower. Novel strategies to induce male sterility and restore fertility in Brassicaceae crops. United States Department of Agriculture, January 2016. http://dx.doi.org/10.32747/2016.7604267.bard.
Анотація:
Abstract Mitochondria are the site of respiration and numerous other metabolic processes required for plant growth and development. Increased demands for metabolic energy are observed during different stages in the plants life cycle, but are particularly ample during germination and reproductive organ development. These activities are dependent upon the tight regulation of the expression and accumulation of various organellar proteins. Plant mitochondria contain their own genomes (mtDNA), which encode for rRNAs, tRNAs and some mitochondrial proteins. Although all mitochondria have probably evolved from a common alpha-proteobacterial ancestor, notable genomic reorganizations have occurred in the mtDNAs of different eukaryotic lineages. Plant mtDNAs are notably larger and more variable in size (ranging from 70~11,000 kbp in size) than the mrDNAs in higher animals (16~19 kbp). Another unique feature of plant mitochondria includes the presence of both circular and linear DNA fragments, which undergo intra- and intermolecular recombination. DNA-seq data indicate that such recombination events result with diverged mitochondrial genome configurations, even within a single plant species. One common plant phenotype that emerges as a consequence of altered mtDNA configuration is cytoplasmic male sterility CMS (i.e. reduced production of functional pollen). The maternally-inherited male sterility phenotype is highly valuable agriculturally. CMS forces the production of F1 hybrids, particularly in predominantly self-pollinating crops, resulting in enhanced crop growth and productivity through heterosis (i.e. hybrid vigor or outbreeding enhancement). CMS lines have been implemented in some cereal and vegetables, but most crops still lack a CMS system. This work focuses on the analysis of the molecular basis of CMS. We also aim to induce nuclear or organellar induced male-sterility in plants, and to develop a novel approach for fertility restoration. Our work focuses on Brassicaceae, a large family of flowering plants that includes Arabidopsis thaliana, a key model organism in plant sciences, as well as many crops of major economic importance (e.g., broccoli, cauliflower, cabbage, and various seeds for oil production). In spite of the genomic rearrangements in the mtDNAs of plants, the number of genes and the coding sequences are conserved among different mtDNAs in angiosperms (i.e. ~60 genes encoding different tRNAs, rRNAs, ribosomal proteins and subunits of the respiratory system). Yet, in addition to the known genes, plant mtDNAs also harbor numerous ORFs, most of which are not conserved among species and are currently of unknown function. Remarkably, and relevant to our study, CMS in plants is primarily associated with the expression of novel chimericORFs, which likely derive from recombination events within the mtDNAs. Whereas the CMS loci are localized to the mtDNAs, the factors that restore fertility (Rfs) are identified as nuclear-encoded RNA-binding proteins. Interestingly, nearly all of the Rf’s are identified as pentatricopeptide repeat (PPR) proteins, a large family of modular RNA-binding proteins that mediate several aspects of gene expression primarily in plant organelles. In this project we proposed to develop a system to test the ability of mtORFs in plants, which are closely related to known CMS factors. We will induce male fertility in various species of Brassicaceae, and test whether a down-relation in the expression of the recombinantCMS-genes restores fertility, using synthetically designed PPR proteins.
3
Whitham, Steven A., Amit Gal-On, and Tzahi Arazi. Functional analysis of virus and host components that mediate potyvirus-induced diseases. United States Department of Agriculture, March 2008. http://dx.doi.org/10.32747/2008.7591732.bard.
Анотація:
The mechanisms underlying the development of symptoms in response to virus infection remain to be discovered in plants. Insight into symptoms induced by potyviruses comes from evidence implicating the potyviral HC-Pro protein in symptom development. In particular, recent studies link the development of symptoms in infected plants to HC-Pro's ability to interfere with small RNA metabolism and function in plant hosts. Moreover, mutation of the highly conserved FRNK amino acid motif to FINK in the HC-Pro of Zucchini yellow mosaic virus (ZYMV) converts a severe strain into an asymptomatic strain, but does not affect virus accumulation in cucurbit hosts. The ability of this FINK mutation to uncouple symptoms from virus accumulation creates a unique opportunity to study symptom etiology, which is usually confounded by simultaneous attenuation of both symptoms and virus accumulation. Our goal was to determine how mutations in the conserved FRNK motif affect host responses to potyvirus infection in cucurbits and Arabidopsis thaliana. Our first objective was to define those amino acids in the FRNK motif that are required for symptoms by mutating the FRNK motif in ZYMV and Turnip mosaic virus (TuMV). Symptom expression and accumulation of resulting mutant viruses in cucurbits and Arabidopsis was determined. Our second objective was to identify plant genes associated with virus disease symptoms by profiling gene expression in cucurbits and Arabidopsis in response to mutant and wild type ZYMV and TuMV, respectively. Genes from the two host species that are differentially expressed led us to focus on a subset of genes that are expected to be involved in symptom expression. Our third objective was to determine the functions of small RNA species in response to mutant and wild type HC-Pro protein expression by monitoring the accumulation of small RNAs and their targets in Arabidopsis and cucurbit plants infected with wild type and mutant TuMV and ZYMV, respectively. We have found that the maintenance of the charge of the amino acids in the FRNK motif of HC-Pro is required for symptom expression. Reduced charge (FRNA, FRNL) lessen virus symptoms, and maintain the suppression of RNA silencing. The FRNK motif is involved in binding of small RNA species including microRNAs (miRNA) and short interfering RNAs (siRNA). This binding activity mediated by the FRNK motif has a role in protecting the viral genome from degradation by the host RNA silencing system. However, it also provides a mechanism by which the FRNK motif participates in inducing the symptoms of viral infection. Small RNA species, such as miRNA and siRNA, can regulate the functions of plant genes that affect plant growth and development. Thus, this binding activity suggests a mechanism by which ZYMVHC-Pro can interfere with plant development resulting in disease symptoms. Because the host genes regulated by small RNAs are known, we have identified candidate host genes that are expected to play a role in symptoms when their regulation is disrupted during viral infections. As a result of this work, we have a better understanding of the FRNK amino acid motif of HC-Pro and its contribution to the functions of HC-Pro, and we have identified plant genes that potentially contribute to symptoms of virus infected plants when their expression becomes misregulated during potyviral infections. The results set the stage to establish the roles of specific host genes in viral pathogenicity. The potential benefits include the development of novel strategies for controlling diseases caused by viruses, methods to ensure stable expression of transgenes in genetically improved crops, and improved potyvirus vectors for expression of proteins or peptides in plants.
4
Lers, Amnon, and Pamela J. Green. LX Senescence-Induced Ribonuclease in Tomato: Function and Regulation. United States Department of Agriculture, September 2003. http://dx.doi.org/10.32747/2003.7586455.bard.
Анотація:
Natural leaf senescence, which occurs even when growth conditions are near optimal, has a negative influence on yield. Postharvest induced senescence contributes to the losses of quality in flowers, foliage, and vegetables. Strategies designed to control the senescence process in crop plants could therefore have great applied significance. However, the successful design of such strategies requires a better insight into the senescence machinery and control in higher plants. A main feature of senescence is the hydrolysis of macromolecules by hydrolases of various types such as ribonucleases (RNases) and proteases. Previously we had identified and characterized the tomato LX RNase gene demonstrating its transcript to be highly and specifically induced during senescence. This reported study was focused on LX but also had broadened our research to other senescence-associated nucleic acids degrading enzymes to learn about their function and the regulation of their encoding genes. Beside tomato we used parsley and Arabidopsis for the study of: the bi-functional nuclease which has a role in senescence. The study of different senescence- associated nucleases in few plant systems will allow a more general view on function and regulation of these enzymes in senescence. The specific original proposed objectives included: 1. Study the consequences of alterations in LX RNase level on tomato leaf senescence and general development; 2. Analyze stimuli which may participate in senescence-specific activation of the LX gene; 3. Clone the senescence-associated BFNI nuclease gene homologue from tomato. 4. Further characterize the sequences required for senescence-specific gene expression. Homozygous transgenic plants in which LX gene was either inhibited or over-expressed were generated. In both of these LX mutated plants no major phenotypic consequences were observed, which may suggests that LX is not essential for plant growth under optimal growth conditions. Lack of any abnormalities in the LX over-expressing lines suggests that special system exist to allow function of the RNase only when needed. Detailed analyses of growth under stress and consequences to RNA metabolism are underway. We have analyzed LX expression on the protein level demonstrating that it is involved also in petal senescing. Our results suggest that LX is responding to complex regulation involving developmental, organ dependent factors and responds differently to hormonal or environmental stimuli in the different plant organs. The cloned 1.4 kb promoter was cloned and its analysis revealed that probably not all required elements for senescence induction are included. Biochemical analysis of senescence-associated be-functional nucleases in the different plants, tomato, parsley and Arabidopsis, suggests they belong to a sub-class within the type I plant nucleases. The parsley PcNUC1/2 nuclease protein was purified from senescing leaves its and activity was studied in vitro revealing endo-, double strand, nucleolytic activity and exo-nucleolytic activity. Its encoding gene was cloned and found to be induced on the mRNA level. The promoter of the related Arabidopsis BFNI nuclease was shown in both tomato and Arabidopsis to be able and direct senescence-specific expression suggesting that, at least part, the gene is regulated on the transcriptional level and that the mechanism for this senescence-specific regulation is conserved between different plants. Few plants in which the BFNI gene is mutated were identified which are subjected now to detailed analysis. Our results suggest that the senescence-related nucleic acid degrading enzymes share similarities in both function and regulation between different plants and possibly have important functions in processes un-related to senescence. Still, the function of these enzymes, at least in some cases is not essential to plant development under optimal growth conditions. We are now at the stage which permits in depth investigation of the specific functions and mode of molecular regulation of senescence-associated nucleases with the aid of the research tools developed. The isolated senescence-specific promoter, shown to be active in heterologous plant system, could be utilized in agricultural-related biotechnological applications for retardation of senescence.
5
Lers, Amnon, and Pamela J. Green. Analysis of Small RNAs Associated with Plant Senescence. United States Department of Agriculture, March 2013. http://dx.doi.org/10.32747/2013.7593393.bard.
Анотація:
Senescence is an agriculturally significant process due to its negative impact to crop yield and postharvest quality. The genetic regulatory systems controlling senescence induction and progress respond to both developmental and environmental stress signals and involve numerous gene expression changes. Knowledge about the key molecular factors which control senescence is very limited. MicroRNAs (miRNAs) are a class of small RNAs which typically function by guiding cleavage of target messenger RNAs. They have been shown to play major roles in a variety of plant processes including development, responses to environmental stresses, and senescence. The long-term goal of this work is to elucidate roles of small RNAs associated with plant senescence. The hypothesis underlying this research is that miRNA-mediated regulation makes important contributions to the senescence process in plants. Specific, original research objectives included: 1) Profiling of small RNAs from senescing plants; 2) Data Analysis and public access via a user-friendly web interface; 3) Validation of senescence-associated miRNAs and target RNAs; 4) Development of transgenic plants for functional analysis of miRNAs in Arabidopsis. Major revisions made in the research compared to the original work plan included 1) Exclusion of the planned work with tomato as recommended by the BARD review panel; 2) Performing miRNA study also in senescing Arabidopsis siliques, in addition to senescing leaves. To identify senescenceregulation of miRNAs in Arabidopsis thaliana, eight small RNA libraries were constructed and sequenced at four different stages of development and senescence from both leaves and siliques, resulting in more than 200 million genome-matched sequences. Parallel Analysis of RNA Ends (PARE) libraries, which enable the large-scale examination of miRNA-guided cleavage products, were also constructed and sequenced, resulting in over 750 million genome-matched sequences. These massive datasets lead to the identification of new miRNAs, as well as new regulation of known miRNAs and their target genes during senescence, many of which have established roles in nutrient responsiveness and cell structural integrity. In keeping with remobilization of nutrients thought to occur during senescence, many miRNAs and targets had opposite expression pattern changes between leaf and silique tissues during the progression of senescence. Taken together, these findings highlight the integral role that miRNAs may play in the remobilization of resources and alteration of cellular structure that is known to occur in senescence. Experiments were initiated for functional analysis of specific senescence-associated miRNAs and respective target genes. Transgenic Arabidopsis plants were generated in which miR408, found in this study to be significantly induced in leaf senescence, was over-expressed either constitutively or under a senescence-specific promoter. These plants are currently being characterized for any altered phenotypes. In addition T-DNA knock out mutants for various target genes identified in this research are being analyzed. This work provides insights about specific miRNAs that contribute to leaf and silique senescence. The knowledge generated may suggest new strategies to monitor and alter the progression of senescence in crops for agricultural improvement.
6
Gafni, Yedidya, and Vitaly Citovsky. Inactivation of SGS3 as Molecular Basis for RNA Silencing Suppression by TYLCV V2. United States Department of Agriculture, November 2013. http://dx.doi.org/10.32747/2013.7593402.bard.
Анотація:
The Israeli isolate of Tomato yellow leaf curl geminivirus(TYLCV-Is) is a major tomato pathogen, causing extensive crop losses in Israel and in the south-eastern U.S. Yet, little is known about the molecular mechanisms of its interaction with tomato cells. One of the most interesting aspects of such interaction is how the invading virus counteracts the RNA silencing response of the plant. In the former BARD project, we have shown that TYLCV-Is V2 protein is an RNA silencing suppressor, and that this suppression is carried out via the interaction of V2 with the SGS3 component of the plant RNA silencing machinery. This reported project was meant to use our data as a foundation to elucidate the molecular mechanism by which V2 affects the SGS3 activity. While this research is likely to have an important impact on our understanding of basic biology of virus-plant interactions and suppression of plant immunity, it also will have practical implications, helping to conceive novel strategies for crop resistance to TYLCV-Is. Our preliminary data in regard to V2 activities and our present knowledge of the SGS3 function suggest likely mechanisms for the inhibitory effect of V2 on SGS3. We have shown that V2 possess structural and functional hallmarks of an F-box protein, suggesting that it may target SGS3 for proteasomal degradation. SGS3 contains an RNA-binding domain and likely functions to protect the cleavage produces of the primary transcript for subsequent conversion to double-stranded forms; thus, V2 may simply block the RNA binding activity of SGS3. V2 may also employ a combination of these mechanisms. These and other possibilities were tested in this reported project.
7
Prusky, Dov B., Tesfaye Mengiste, and Robert Fluhr. Mechanisms activated by fungal-based host pH modulators during quiescent infections and active postharvest disease development. United States Department of Agriculture, 2011. http://dx.doi.org/10.32747/2011.7597911.bard.
Анотація:
This project aims were to provide new insights on the mechanisms activated during alkalinization and acidification of the infection court by Colletotrichum and Botrytis spp. respectively that will lead to quiescent infection-development on tomato fruits. We have chosen these pathogens due to their contrasting life style of alkalinization and acidification, respectively. We will study the roles of these fungal-based host-pH modulators in modulating host gene expression during quiescent infection development and compare these roles with those governing active colonization as a basis for developing novel strategies for control of postharvest diseases. The aims will be pursued through: Characterization of the effects of pH modulation on fungal-plant cell-cell signaling and on the fungal and plant transcriptome during the initial stages of fungal quiescence. The unpublished material that is presented as short abstract is considered one of the key point modulating Characterization of expression profiles of tomato fruits affected by acidifying and alkalinizing pathogensduring the transformation of quiescent to active infections by Colletotrichum and Botrytis. Functional analysis of selected genes involved in signaling pathways that affects the quiescent and active infections of Colletotrichum and Botrytis.
8
Li, Li, Joseph Burger, Nurit Katzir, Yaakov Tadmor, Ari Schaffer, and Zhangjun Fei. Characterization of the Or regulatory network in melon for carotenoid biofortification in food crops. United States Department of Agriculture, April 2015. http://dx.doi.org/10.32747/2015.7594408.bard.
Анотація:
The general goals of the BARD research grant US-4423-11 are to understand how Or regulates carotenoid accumulation and to reveal novel strategies for breeding agricultural crops with enhanced β-carotene level. The original objectives are: 1) to identify the genes and proteins in the Or regulatory network in melon; 2) to genetically and molecularly characterize the candidate genes; and 3) to define genetic and functional allelic variation of these genes in a representative germplasm collection of the C. melo species. Or was found by the US group to causes provitamin A accumulation in chromoplasts in cauliflower. Preliminary genetic study from the Israeli group revealed that the melon Or gene (CmOr) completely co-segregated with fruit flesh color in a segregating mapping population and in a wide melon germplasm collection, which set the stage for the funded research. Major conclusions and achievements include: 1). CmOris proved to be the gene that controls melon fruit flesh color and represents the previously described gflocus in melon. 2). Genetic and molecular analyses of CmOridentify and confirm a single SNP that is responsible for the orange and non-orange phenotypes in melon fruit. 3). Alteration of the evolutionarily conserved arginine in an OR protein to both histidine or alanine greatly enhances its ability to promote carotenoid accumulation. 4). OR promotes massive carotenoid accumulation due to its dual functions in regulating both chromoplast biogenesis and carotenoid biosynthesis. 5). A bulk segregant transcriptome (BSRseq) analysis identifies a list of genes associated with the CmOrregulatory network. 6). BSRseq is proved to be an effective approach for gene discovery. 7). Screening of an EMS mutation library identifies a low β mutant, which contains low level of carotenoids due to a mutation in CmOrto produce a truncated form of OR protein. 8). low β exhibits lower germination rate and slow growth under salt stress condition. 9). Postharvest storage of fruit enhances carotenoid accumulation, which is associated with chromoplast development. Our research uncovers the molecular mechanisms underlying the Or-regulated high level of carotenoid accumulation via regulating carotenoidbiosynthetic capacity and storage sink strength. The findings provide mechanistic insights into how carotenoid accumulation is controlled in plants. Our research also provides general and reliable molecular markers for melon-breeding programs to select orange varieties, and offers effective genetic tools for pro-vitamin A enrichment in other important crops via the rapidly developed genome editing technology. The newly discovered low β mutant could lead to a better understanding of the Or gene function and its association with stress response, which may explain the high conservation of the Or gene among various plant species.
9
Levy, Maggie, Raymond Zielinski, and Anireddy S. Reddy. IQD1 Function in Defense Responses. United States Department of Agriculture, January 2012. http://dx.doi.org/10.32747/2012.7699842.bard.
Анотація:
The main objective of the proposed research was to study IQD1's mechanism of action and elucidate its role in plant protection. Preliminary experiments suggest that IQD1 binds CaM in a Ca²⁺-dependent manner and functions in general defense responses. We propose to identify proteins and genes that interact with IQD1, which may provide some clues to its mechanism of action. We also plan to dissect IQD1's integration in defense pathways and to study and modulate its binding affinity to CaM in order to enhance crop resistance. Our specific objectives were: (1) Analysis of IQD1's CaM-binding properties; (2) Identification of IQD1 targets;(3) Dissection of IQD1 integration into defense signaling pathways. Analysis of IQD1's CaM-binding properties defined four potential classes of sequences that should affect CaM binding: one is predicted to raise the affinity for Ca²⁺-dependent interaction but have no effect on Ca²⁺-independent binding; a second is predicted to act like the first mutation but eliminate Ca²⁺-independent binding; a third has no predicted effect on Ca²⁺-dependent binding but eliminates Ca²⁺-independent binding; and the fourth is predicted to eliminate or greatly reduce both Ca²⁺-dependent and Ca²⁺-independent binding. Following yeast two hybrid analysis we found that IQD1 interact with AtSR1 (Arabidopsis thalianaSIGNALRESPONSIVE1), a calcium/calmodulin-binding transcription factor, which has been shown to play an important role in biotic and abiotic stresses. We tested IQD1 interaction with both N-terminal or C-terminal half of SR1. These studies have uncovered that only the N-terminal half of the SR1 interacts with the IQD1. Since IQD1 has an important role in herbivory, its interaction with SR1 suggests that it might also be involved in plant responses to insect herbivory. Since AtSR1, like IQD1, is a calmodulin-binding protein and the mutant showed increased sensitivity to a herbivore, we analyzed WT, Atsr1 and the complemented line for the levels of GS to determine if the increased susceptibility of Atsr1 plants to T. ni feeding is associated with altered GS content. In general, Atsr1 showed a significant reduction in both aliphatic and aromatic GS levels as compared to WT. In order to study IQD1's molecular basis integration into hormone-signaling pathways we tested the epistatic relationships between IQD1 and hormone-signaling mutants. For that purpose we construct double mutants between IQD1ᴼXᴾ and mutants defective in plant-hormone signaling and GS accumulation. Epitasis with SA mutant NahG and npr1-1 and JA mutant jar1-1 suggested IQD1 function is dependent on both JA and SA as indicated by B. cinerea infection assays. We also verified the glucosinolate content in the crosses siblings and found that aliphatic GSL content is reduced in the double transgenic plants NahG:IQD1ᴼXᴾ as compare to parental lines while the aliphatic GSL content in the npr1-1:IQD1ᴼXᴾ and jar1-1: IQD1ᴼXᴾ double mutants was intimidated to the parental lines. This suggests that GSL content dependency on SA is downstream to IQD1. As a whole, this project should contribute to the development of new defense strategies that will improve crop protection and reduce yield losses and the amount of pesticides required; these will genuinely benefit farmers, consumers and the environment.
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Christopher, David A., and Avihai Danon. Plant Adaptation to Light Stress: Genetic Regulatory Mechanisms. United States Department of Agriculture, May 2004. http://dx.doi.org/10.32747/2004.7586534.bard.
Анотація:
Original Objectives: 1. Purify and biochemically characterize RB60 orthologs in higher plant chloroplasts; 2. Clone the gene(s) encoding plant RB60 orthologs and determine their structure and expression; 3. Manipulate the expression of RB60; 4. Assay the effects of altered RB60 expression on thylakoid biogenesis and photosynthetic function in plants exposed to different light conditions. In addition, we also examined the gene structure and expression of RB60 orthologs in the non-vascular plant, Physcomitrella patens and cloned the poly(A)-binding protein orthologue (43 kDa RB47-like protein). This protein is believed to a partner that interacts with RB60 to bind to the psbA5' UTR. Thus, to obtain a comprehensive view of RB60 function requires analysis of its biochemical partners such as RB43. Background & Achievements: High levels of sunlight reduce photosynthesis in plants by damaging the photo system II reaction center (PSII) subunits, such as D1 (encoded by the chloroplast tpsbAgene). When the rate of D1 synthesis is less than the rate of photo damage, photo inhibition occurs and plant growth is decreased. Plants use light-activated translation and enhanced psbAmRNA stability to maintain D1 synthesis and replace the photo damaged 01. Despite the importance to photosynthetic capacity, these mechanisms are poorly understood in plants. One intriguing model derived from the algal chloroplast system, Chlamydomonas, implicates the role of three proteins (RB60, RB47, RB38) that bind to the psbAmRNA 5' untranslated leader (5' UTR) in the light to activate translation or enhance mRNA stability. RB60 is the key enzyme, protein D1sulfide isomerase (Pill), that regulates the psbA-RN :Binding proteins (RB's) by way of light-mediated redox potentials generated by the photosystems. However, proteins with these functions have not been described from higher plants. We provided compelling evidence for the existence of RB60, RB47 and RB38 orthologs in the vascular plant, Arabidopsis. Using gel mobility shift, Rnase protection and UV-crosslinking assays, we have shown that a dithiol redox mechanism which resembles a Pill (RB60) activity regulates the interaction of 43- and 30-kDa proteins with a thermolabile stem-loop in the 5' UTR of the psbAmRNA from Arabidopsis. We discovered, in Arabidopsis, the PD1 gene family consists of II members that differ in polypeptide length from 361 to 566 amino acids, presence of signal peptides, KDEL motifs, and the number and positions of thioredoxin domains. PD1's catalyze the reversible formation an disomerization of disulfide bonds necessary for the proper folding, assembly, activity, and secretion of numerous enzymes and structural proteins. PD1's have also evolved novel cellular redox functions, as single enzymes and as subunits of protein complexes in organelles. We provide evidence that at least one Pill is localized to the chloroplast. We have used PDI-specific polyclonal and monoclonal antisera to characterize the PD1 (55 kDa) in the chloroplast that is unevenly distributed between the stroma and pellet (containing membranes, DNA, polysomes, starch), being three-fold more abundant in the pellet phase. PD1-55 levels increase with light intensity and it assembles into a high molecular weight complex of ~230 kDa as determined on native blue gels. In vitro translation of all 11 different Pill's followed by microsomal membrane processing reactions were used to differentiate among PD1's localized in the endoplasmic reticulum or other organelles. These results will provide.1e insights into redox regulatory mechanisms involved in adaptation of the photosynthetic apparatus to light stress. Elucidating the genetic mechanisms and factors regulating chloroplast photosynthetic genes is important for developing strategies to improve photosynthetic efficiency, crop productivity and adaptation to high light environments.