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Статті в журналах з теми "Live Cell Imaging Biosensors"
Yoon, Sangpil, Yijia Pan, Kirk Shung, and Yingxiao Wang. "FRET-Based Ca2+ Biosensor Single Cell Imaging Interrogated by High-Frequency Ultrasound." Sensors 20, no. 17 (September 3, 2020): 4998. http://dx.doi.org/10.3390/s20174998.
Повний текст джерелаSnell, Nicole, Vishnu Rao, Kendra Seckinger, Junyi Liang, Jenna Leser, Allison Mancini, and M. Rizzo. "Homotransfer FRET Reporters for Live Cell Imaging." Biosensors 8, no. 4 (October 11, 2018): 89. http://dx.doi.org/10.3390/bios8040089.
Повний текст джерелаSecilmis, Melike, Hamza Yusuf Altun, Johannes Pilic, Yusuf Ceyhun Erdogan, Zeynep Cokluk, Busra Nur Ata, Gulsah Sevimli, et al. "A Co-Culture-Based Multiparametric Imaging Technique to Dissect Local H2O2 Signals with Targeted HyPer7." Biosensors 11, no. 9 (September 14, 2021): 338. http://dx.doi.org/10.3390/bios11090338.
Повний текст джерелаTiruthani, Karthik, Adam Mischler, Shoeb Ahmed, Jessica Mahinthakumar, Jason M. Haugh, and Balaji M. Rao. "Design and evaluation of engineered protein biosensors for live-cell imaging of EGFR phosphorylation." Science Signaling 12, no. 584 (June 4, 2019): eaap7584. http://dx.doi.org/10.1126/scisignal.aap7584.
Повний текст джерелаHouser, Mei CQ, Steven S. Hou, Florian Perrin, Yuliia Turchyna, Brian J. Bacskai, Oksana Berezovska та Masato Maesako. "A Novel NIR-FRET Biosensor for Reporting PS/γ-Secretase Activity in Live Cells". Sensors 20, № 21 (22 жовтня 2020): 5980. http://dx.doi.org/10.3390/s20215980.
Повний текст джерелаWoehler, Andrew. "Simultaneous Quantitative Live Cell Imaging of Multiple FRET-Based Biosensors." PLoS ONE 8, no. 4 (April 16, 2013): e61096. http://dx.doi.org/10.1371/journal.pone.0061096.
Повний текст джерелаVilchez Mercedes, Samuel A., Ian Eder, Mona Ahmed, Ninghao Zhu, and Pak Kin Wong. "Optimizing locked nucleic acid modification in double-stranded biosensors for live single cell analysis." Analyst 147, no. 4 (2022): 722–33. http://dx.doi.org/10.1039/d1an01802g.
Повний текст джерелаBanerjee, Swayoma, Luis Rene Garcia, and Wayne K. Versaw. "Quantitative Imaging of FRET-Based Biosensors for Cell- and Organelle-Specific Analyses in Plants." Microscopy and Microanalysis 22, no. 2 (February 16, 2016): 300–310. http://dx.doi.org/10.1017/s143192761600012x.
Повний текст джерелаDobrzyński, Maciej, Marc-Antoine Jacques, and Olivier Pertz. "Mining single-cell time-series datasets with Time Course Inspector." Bioinformatics 36, no. 6 (November 14, 2019): 1968–69. http://dx.doi.org/10.1093/bioinformatics/btz846.
Повний текст джерелаValetdinova, Kamila R., Tuyana B. Malankhanova, Suren M. Zakian, and Sergey P. Medvedev. "The Cutting Edge of Disease Modeling: Synergy of Induced Pluripotent Stem Cell Technology and Genetically Encoded Biosensors." Biomedicines 9, no. 8 (August 5, 2021): 960. http://dx.doi.org/10.3390/biomedicines9080960.
Повний текст джерелаДисертації з теми "Live Cell Imaging Biosensors"
Konishi, Yoshinobu. "Live-cell FRET imaging reveals a role of extracellular signal-regulated kinase activity dynamics in thymocyte motility." Kyoto University, 2019. http://hdl.handle.net/2433/242374.
Повний текст джерелаHung, Yin Pun. "Single Cell Imaging of Metabolism with Fluorescent Biosensors." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10147.
Повний текст джерелаKosmacek, Elizabeth Anne Ianzini Fiorenza Mackey Michael A. "Live cell imaging technology development for cancer research." [Iowa City, Iowa] : University of Iowa, 2009. http://ir.uiowa.edu/etd/388.
Повний текст джерелаChyan, Wen Ph D. Massachusetts Institute of Technology. "Fluorogenic probes for live-cell imaging of biomolecules." Thesis, Massachusetts Institute of Technology, 2018. http://hdl.handle.net/1721.1/118216.
Повний текст джерелаCataloged from PDF version of thesis.
Includes bibliographical references (pages 231-249).
Fluorogenic probes, small-molecule sensors that unmask brilliant fluorescence upon exposure to specific stimuli, are essential tools for chemical biology. Probes that detect enzymatic activity can be used to illuminate the complex dynamics of biological processes at a level of spatiotemporal detail and sensitivity unmatched by other techniques. This dissertation describes the development of new fluorophore chemistries to expand our current fluorogenic probe toolkit and the subsequent application of these probes to study dynamic cell transport processes. Chapter 1. Enzyme-Activated Fluorogenic Probes for Live-Cell and In Vivo Imaging. Chapter 1 reviews recent advances in enzyme-activated fluorogenic probes for biological imaging, organized by enzyme classification. This review surveys recent masking strategies, different modes of enzymatic activation, and the breadth of current and future probe applications. Key challenges, such as probe selectivity and spectroscopic requirements, are described in this chapter along with therapeutic and diagnostic opportunities that can be accessed by surmounting these challenges. Chapter 2. Electronic and Steric Optimization of Fluorogenic Probes for Biomolecular Imaging. In many fluorogenic probes, the intrinsic fluorescence of a small-molecule fluorophore is masked by ester masking groups until entry into a cell, where endogenous esterases catalyze the hydrolysis of esters, generating fluorescence. The susceptibility of masking groups to spontaneous hydrolysis is a major limitation of these probes. Previous attempts to address this problem have incorporated auto-immolative linkers at the cost of atom economy and synthetic adversity. In this chapter, I report on a linker-free strategy that employs adventitious electronic and steric interactions in easy-to-synthesize probes. I find that halogen-carbonyl n-->[pi]* interactions and acyl group size are optimized in 2',7'-dichlorofluorescein diisobutyrate. This probe is relatively stable to spontaneous hydrolysis but is a highly reactive substrate for esterases both in vitro and in cellulo, yielding a bright, photostable fluorogenic probe with utility in biomolecular imaging. Chapter 3. Cellular Uptake of Large Monofunctionalized Dextrans. Dextrans are a versatile class of polysaccharides with applications that span medicine, cell biology, food science, and consumer goods. In Chapter 3, I apply the electronically stabilized probe described in Chapter 2 to study the cellular uptake of a new type of large monofunctionalized dextran that exhibits unusual properties: efficient cytosolic and nuclear uptake. This dextran permeates various human cell types without the use of transfection agents, electroporation, or membrane perturbation. Cellular uptake occurs primarily through active transport via receptor-mediated processes. These monofunctionalized dextrans could serve as intracellular delivery platforms for drugs or other cargos. Chapter 4. Paired Nitroreductase-Probe System to Quantify the Cytosolic Delivery of Biomolecules. Cytosolic delivery of large biomolecules is a significant barrier to therapeutic applications of CRISPR, RNAi, and biologics such as proteins with anticancer properties. In Chapter 4, I describe a new paired enzyme-probe system to quantify cytosolic delivery of biomolecules-a valuable resource for elucidating mechanistic details and improving delivery of therapeutics. I designed and optimized a nitroreductase fusion protein that embeds in the cytosolic face of outer mitochondrial membranes, providing several key improvements over unanchored reporter enzymes. In parallel, I prepared and assessed a panel of nitroreductase-activated probes for favorable spectroscopic and enzymatic activation properties. Together, the nitroreductase fusion protein and fluorogenic probes provide a rapid, generalizable tool that is well-poised to quantify cytosolic delivery of biomolecules. Chapter 5. Future Directions. This chapter outlines several future directions for expanding the scope of fluorogenic probes and developing new biological applications. Additionally, Chapter 5 is followed by an appendix describing a tunable rhodol fluorophore scaffold for improved spectroscopic properties and versatility. Overall, the work described in this thesis illustrates the power of enzyme-activated fluorogenic probes to provide fresh insight into dynamic biological processes, with direct implications for improved therapeutic delivery.
by Wen Chyan.
Ph. D. in Biological Chemistry
Büchele, Benjamin. "Live Cell Imaging des Hepatitis C Virus Replikationskomplexes." [S.l. : s.n.], 2004. http://nbn-resolving.de/urn:nbn:de:bsz:25-opus-59102.
Повний текст джерелаKosmacek, Elizabeth Anne. "Live cell imaging technology development for cancer research." Diss., University of Iowa, 2009. https://ir.uiowa.edu/etd/388.
Повний текст джерелаCaporale, Chiara. "Luminescent Iridium Tetrazolato Markers for Live Cell Imaging." Thesis, Curtin University, 2018. http://hdl.handle.net/20.500.11937/70386.
Повний текст джерелаDanylchuk, Dmytro. "Environment-sensitive targeted fluorescent probes for live-cell imaging." Thesis, Strasbourg, 2021. http://www.theses.fr/2021STRAF012.
Повний текст джерелаSpecific targeting, imaging and probing of cell plasma membranes and intracellular organelles can be addressed by rationally designed polarity-sensitive fluorescent probes. Here, a new efficient plasma membrane-targeting moiety was developed and tested in five cyanine dyes, showing excellent performance in cellular and in vivo microscopy. Next, the targeting moiety was grafted to a solvatochromic dye Prodan, yielding a plasma membrane probe with high lipid order sensitivity. Modifying a Nile Red using the moieties with varied alkyl chain lengths resulted in two solvatochromic plasma membrane probes: NR12A with high affinity to membranes for conventional microscopy, and NR4A, a low-affinity probe for PAINT super-resolution microscopy. Tethering Nile Red with organelle-targeted groups yielded an array of probes, able to sense polarity and lipid order in organelle membranes. The synthesized probes will find applications in bioimaging, cell biology, biophysics or mechanobiology
Sörman, Paulsson Elsa. "Evaluation of In-Silico Labeling for Live Cell Imaging." Thesis, Umeå universitet, Institutionen för fysik, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-180590.
Повний текст джерелаHan, Hongqing. "Towards accurate and efficient live cell imaging data analysis." Doctoral thesis, Humboldt-Universität zu Berlin, 2021. http://dx.doi.org/10.18452/22324.
Повний текст джерелаLive cell imaging based on time-lapse microscopy has been used to study dynamic cellular behaviors, such as cell cycle, cell signaling and transcription. Extracting cell lineage trees out of a time-lapse video requires cell segmentation and cell tracking. For long term live cell imaging, data analysis errors are particularly fatal. Even an extremely low error rate could potentially be amplified by the large number of sampled time points and render the entire video useless. In this work, we adopt a straightforward but practical design that combines the merits of manual and automatic approaches. We present a live cell imaging data analysis tool `eDetect', which uses post-editing to complement automatic segmentation and tracking. What makes this work special is that eDetect employs multiple interactive data visualization modules to guide and assist users, making the error detection and correction procedure rational and efficient. Specifically, two scatter plots and a heat map are used to interactively visualize single cells' visual features. The scatter plots position similar results in close vicinity, making it easy to spot and correct a large group of similar errors with a few mouse clicks, minimizing repetitive human interventions. The heat map is aimed at exposing all overlooked errors and helping users progressively approach perfect accuracy in cell lineage reconstruction. Quantitative evaluation proves that eDetect is able to largely improve accuracy within an acceptable time frame, and its performance surpasses the winners of most tasks in the `Cell Tracking Challenge', as measured by biologically relevant metrics.
Книги з теми "Live Cell Imaging Biosensors"
Kim, Sung-Bae, ed. Live Cell Imaging. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1258-3.
Повний текст джерелаPapkovsky, Dmitri B., ed. Live Cell Imaging. Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-404-3.
Повний текст джерелаLive cell imaging: Methods and protocols. New York, NY: Humana Press, 2010.
Знайти повний текст джерела1939-, Goldman Robert D., Swedlow Jason, and Spector David L, eds. Live cell imaging: A laboratory manual. 2nd ed. Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory Press, 2010.
Знайти повний текст джерелаLive cell imaging: A laboratory manual. 2nd ed. Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory Press, 2010.
Знайти повний текст джерела1939-, Goldman Robert D., Swedlow Jason, and Spector David L, eds. Live cell imaging: A laboratory manual. 2nd ed. Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory Press, 2010.
Знайти повний текст джерелаMasters, Barry R. Confocal microscopy and multiphoton excitation microscopy: The genesis of live cell imaging. Bellingham, WA: SPIE Press, 2006.
Знайти повний текст джерелаConfocal microscopy and multiphoton excitation microscopy: The genesis of live cell imaging. Bellingham, Wash: SPIE Press, 2005.
Знайти повний текст джерелаTsukahara, Shinji. Kagaku busshitsu no yūgaisei hyōka no kōritsuka o mezashita aratana shinkei dokusei shikenhō no kaihatsu: Kankyōshō kankyō kenkyū gijutsu kaihatsu suishinhi shūryō kenkyū seika hōkokusho : Heisei 20-nendo--Heisei 21-nendo = Use of live cell imaging for efficient neurotoxicology methods : environment research and technology development fund. [Tokyo]: Kankyōshō Sōgō Kankyō Seisakukyoku Kankyō Hokenbu Kankyō Anzenka Kankyō Risuku Hyōkashitsu, 2010.
Знайти повний текст джерелаTsukahara, Shinji. Kagaku busshitsu no yūgaisei hyōka no kōritsuka o mezashita aratana shinkei dokusei shikenhō no kaihatsu: Kankyōshō kankyō kenkyū gijutsu kaihatsu suishinhi shūryō kenkyū seika hōkokusho : Heisei 20-nendo--Heisei 21-nendo = Use of live cell imaging for efficient neurotoxicology methods : environment research and technology development fund. [Tokyo]: Kankyōshō Sōgō Kankyō Seisakukyoku Kankyō Hokenbu Kankyō Anzenka Kankyō Risuku Hyōkashitsu, 2010.
Знайти повний текст джерелаЧастини книг з теми "Live Cell Imaging Biosensors"
Bravo-Cordero, Jose Javier, Yasmin Moshfegh, John Condeelis, and Louis Hodgson. "Live Cell Imaging of RhoGTPase Biosensors in Tumor Cells." In Adhesion Protein Protocols, 359–70. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-538-5_22.
Повний текст джерелаSato, Moritoshi. "Genetically Encoded Fluorescent Biosensors for Live Cell Imaging of Lipid Dynamics." In Methods in Molecular Biology, 73–81. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-622-1_6.
Повний текст джерелаMishina, Natalia M., and Vsevolod V. Belousov. "Live-Cell STED Imaging with the HyPer2 Biosensor." In Methods in Molecular Biology, 21–28. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7265-4_3.
Повний текст джерелаKellenberger, Colleen A., Zachary F. Hallberg, and Ming C. Hammond. "Live Cell Imaging Using Riboswitch-Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensors." In RNA Scaffolds, 87–103. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2730-2_8.
Повний текст джерелаOuyang, Mingxing, Shaoying Lu, and Yingxiao Wang. "Genetically Encoded Fluorescent Biosensors for Live-Cell Imaging of MT1-MMP Protease Activity." In Methods in Molecular Biology, 163–74. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-622-1_13.
Повний текст джерелаManna, Sudeshna, Colleen A. Kellenberger, Zachary F. Hallberg, and Ming C. Hammond. "Live Cell Imaging Using Riboswitch–Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensors." In RNA Scaffolds, 121–40. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1499-0_10.
Повний текст джерелаHung, Yin Pun, and Gary Yellen. "Live-Cell Imaging of Cytosolic NADH–NAD+ Redox State Using a Genetically Encoded Fluorescent Biosensor." In Methods in Molecular Biology, 83–95. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-622-1_7.
Повний текст джерелаLopreside, Antonia, Maria Maddalena Calabretta, Laura Montali, Aldo Roda, and Elisa Michelini. "Live Cell Immobilization." In Handbook of Cell Biosensors, 1–18. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-319-47405-2_146-1.
Повний текст джерелаLopreside, Antonia, Maria Maddalena Calabretta, Laura Montali, Aldo Roda, and Elisa Michelini. "Live Cell Immobilization." In Handbook of Cell Biosensors, 479–96. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-23217-7_146.
Повний текст джерелаVan Kerckvoorde, Melinda, Matthew J. Ford, Patricia L. Yeyati, Pleasantine Mill, and Richard L. Mort. "Live Imaging and Analysis of Cilia and Cell Cycle Dynamics with the Arl13bCerulean-Fucci2a Biosensor and Fucci Tools." In Methods in Molecular Biology, 291–309. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1538-6_21.
Повний текст джерелаТези доповідей конференцій з теми "Live Cell Imaging Biosensors"
Pavlinska, Zuzana, Zuzana Kronekova, Igor Lacik, Dusana Trelova, Filip Razga, Veronika Nemethova, Lucia Uhelska, Alzbeta Marcek Chorvatova, Tibor Teplicky, and Dusan Chorvat. "A bio-inspired design of live cell biosensors." In Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XV, edited by Alexander N. Cartwright, Dan V. Nicolau, and Dror Fixler. SPIE, 2018. http://dx.doi.org/10.1117/12.2288789.
Повний текст джерелаLu, Shaoying, and Yingxiao Wang. "Application of FRET biosensors and computational analysis for live cell imaging." In SPIE BiOS: Biomedical Optics, edited by Alexander P. Savitsky and Yingxiao Wang. SPIE, 2009. http://dx.doi.org/10.1117/12.812183.
Повний текст джерелаKamioka, Yuji, Kenta Sumiyama, Rei Mizuno, and Michiyuki Matsuda. "Live imaging of transgenic mice expressing FRET biosensors." In 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2013. http://dx.doi.org/10.1109/embc.2013.6609453.
Повний текст джерелаBoero, Cristina, Maria Antonietta Casulli, Jacopo Olivo, Lorenzo Foglia, Sandro Carrara, and Giovanni De Micheli. "Live demonstration: In-situ biosensors array for cell culture monitoring." In 2014 IEEE Biomedical Circuits and Systems Conference (BioCAS). IEEE, 2014. http://dx.doi.org/10.1109/biocas.2014.6981676.
Повний текст джерелаMoscelli, Nicola, Wojciech Witarski, Sander van den Driesche, and Michael J. Vellekoop. "In incubator live cell imaging platform." In SPIE Microtechnologies, edited by Ulrich Schmid, José Luis Sánchez-Rojas, and Monika Leester-Schaedel. SPIE, 2011. http://dx.doi.org/10.1117/12.886908.
Повний текст джерелаSchneckenburger, Herbert, Verena Richter, Sarah Bruns, Thomas Bruns, Mathis Piper, Petra Weber, Michael Wagner, and Christoph Cremer. "Axial tomography in 3D live cell microscopy." In Advances in Microscopic Imaging, edited by Francesco S. Pavone, Emmanuel Beaurepaire, and Peter T. So. SPIE, 2017. http://dx.doi.org/10.1117/12.2286602.
Повний текст джерелаRen, Juan, and Qingze Zou. "Modeling of sample deformation in atomic force microscope imaging on live cell: Live mammalian cell imaging example?" In 2017 American Control Conference (ACC). IEEE, 2017. http://dx.doi.org/10.23919/acc.2017.7963048.
Повний текст джерелаSim, J. Y., N. Borghi, A. Ribeiro, M. Sorokina, O. Shcherbakova, D. Ramallo, A. Dunn, W. J. Nelson, and B. L. Pruitt. "Uniaxial cell stretcher enables high resolution live cell imaging." In 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2012. http://dx.doi.org/10.1109/memsys.2012.6170320.
Повний текст джерелаMellors, Ben O., Hamid Dehghani, Christopher R. Howle, and Abigail M. Spear. "Cell trauma detection using infra-red live cell imaging." In Preclinical and Clinical Optical Diagnostics, edited by J. Quincy Brown and Ton G. van Leeuwen. SPIE, 2019. http://dx.doi.org/10.1117/12.2525012.
Повний текст джерелаSchneckenburger, H., V. Richter, and C. Cremer. "Multi-modal Imaging in Live Cell Microscopy." In 2020 International Conference Laser Optics (ICLO). IEEE, 2020. http://dx.doi.org/10.1109/iclo48556.2020.9285496.
Повний текст джерелаЗвіти організацій з теми "Live Cell Imaging Biosensors"
Ray, Judhajeet. Aptamer sensors for live-cell imaging of Pol II promoter activity. Office of Scientific and Technical Information (OSTI), December 2014. http://dx.doi.org/10.2172/1227286.
Повний текст джерелаZhang, Yun. Real time imaging of live cell ATP leaking or release events by chemiluminescence microscopy. Office of Scientific and Technical Information (OSTI), December 2008. http://dx.doi.org/10.2172/964390.
Повний текст джерелаBelkin, Shimshon, Sylvia Daunert, and Mona Wells. Whole-Cell Biosensor Panel for Agricultural Endocrine Disruptors. United States Department of Agriculture, December 2010. http://dx.doi.org/10.32747/2010.7696542.bard.
Повний текст джерела