Готові списки джерел за темами / Laser Fluorescence Imaging

Добірка наукової літератури з теми "Laser Fluorescence Imaging"

Автор: Grafiati
Опубліковано: 6 вересня 2023

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Статті в журналах з теми "Laser Fluorescence Imaging"

1

Hanson, Ronald K. "Planar laser-induced fluorescence imaging." Journal of Quantitative Spectroscopy and Radiative Transfer 40, no. 3 (September 1988): 343–62. http://dx.doi.org/10.1016/0022-4073(88)90125-2.

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2

Saitoh, Naoki, and Norimitsu Akiba. "Ultraviolet Fluorescence Imaging of Fingerprints." Scientific World JOURNAL 6 (2006): 691–99. http://dx.doi.org/10.1100/tsw.2006.143.

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We studied fluorescence imaging of fingerprints on a high-grade white paper in the deep ultraviolet (UV) region with a nanosecond-pulsed Nd-YAG laser system that consists of a tunable laser and a cooled CCD camera.Clear fluorescence images were obtained by time-resolved imaging with a 255- to 425-nm band-pass filter, which cuts off strong fluorescence of papers. Although fluorescence can be imaged with any excitation wavelength between 220 and 290 nm, 230 and 280 nm are the best in terms of image quality. However, the damage due to laser illumination was smaller for 266-nm excitation than 230- or 280-nm excitation.Absorption images of latent fingerprints on a high-grade white paper are also obtained with our imaging system using 215- to 280-nm laser light. Shorter wavelengths produce better images and the best image was obtained with 215 nm. Absorption images are also degraded slightly by laser illumination, but their damage is smaller than that of fluorescence images.
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3

Cappelli, M. A., P. H. Paul, and R. K. Hanson. "Laser‐induced fluorescence imaging of laser‐ablated barium." Applied Physics Letters 56, no. 18 (April 30, 1990): 1715–17. http://dx.doi.org/10.1063/1.103124.

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4

Gupta, Neelam. "Spectropolarimetric Imaging of Laser-Induced Fluorescence." IEEE Sensors Journal 10, no. 3 (March 2010): 503–8. http://dx.doi.org/10.1109/jsen.2009.2038189.

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5

Grönlund, Rasmus, Jenny Hällström, Ann Johansson, Kerstin Barup, and Sune Svanberg. "Remote Multicolor Excitation Laser-Induced Fluorescence Imaging." Laser Chemistry 2006 (January 10, 2006): 1–6. http://dx.doi.org/10.1155/2006/57934.

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Remote laser-induced fluorescence of stone materials was performed with application towards cultural heritage. Fluorescence was induced in targets ∼60 m from a mobile lidar laboratory by ultraviolet laser light, either from a frequency-tripled Nd:YAG laser or from an optical parametric oscillator system. Analysis was performed on combined spectra from the different excitation wavelengths and it was noted that important additional information can be gained when using several excitation wavelengths.
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6

Clark Brelje, T., and Robert L. Sorenson. "Multi-color laser scanning confocal microscopy with a krypton/argon ion laser." Proceedings, annual meeting, Electron Microscopy Society of America 49 (August 1991): 406–7. http://dx.doi.org/10.1017/s0424820100086337.

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Fluorescence is presently the most important imaging mode in biological confocal microscopy. The optical properties of laser scanning confocal microscopy (LSCM) are particularly favorable for fluorescence microscopy since the generally high signal-to-background ratio is enhanced by LSCM by rejecting out-of-focus fluorescent emissions. In addition, this improved imaging capability along the optical (z-)axis allows the optical sectioning of specimens by adjusting the plane of focus. This removes one of the most severe limitations of convential fluorescence microscopy, the necessity to examine monolayers of cells or thin sections of tissues.However, the advantages of LSCM for multi-color fluorscence microscopy are critically dependent on the availability of suitable light sources and fluorophores. By far the most commonly used light source is a small, air-cooled argon ion laser with emission wavelengths at 488 and 514 nm. Although the most frequently used fluorophores, fluorescein (FITC) and tetramethylrhodamine, can be excited by these wavelengths, it is impossible to specifically excite each fluorophore in the presence of the other fluorophore.
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7

Tan, Weihong, Philip G. Haydon, and Edward S. Yeung. "Imaging Neurotransmitter Uptake and Depletion in Astrocytes." Applied Spectroscopy 51, no. 8 (August 1997): 1139–43. http://dx.doi.org/10.1366/0003702971941656.

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An ultraviolet (UV) laser-based optical microscope and charge-coupled device (CCD) detection system was used to obtain chemical images of biological cells. Subcellularstructures can be easily seen in both optical and fluorescence images. Laser-induced native fluorescence detection provides high sensitivity and low limits of detection, and it does not require coupling to fluorescent dyes. We were able to quantitatively monitor serotonin that has been taken up into and released from individual astrocytes on the basis of its native fluorescence. Different regions of the cells took up different amounts of serotonin with a variety of uptake kinetics. Similarly, we observed different serotonin depletion dynamics in different astrocyte regions. There were also some astrocyte areas where no serotonin uptake or depletion was observed. Potential applications include the mapping of other biogenic species in cells as well as the ability to image their release from specific regions of cells in response to external stimuli.
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8

Häkkänen, H. J., and J. E. I. Korppi-Tommola. "Laser-Induced Fluorescence Imaging of Paper Surfaces." Applied Spectroscopy 47, no. 12 (December 1993): 2122–25. http://dx.doi.org/10.1366/0003702934066307.

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Laser-induced fluorescence imaging has been used to study the microstructure of paper surfaces. Pulses from a XeCl-excimer laser, 10 ns in duration at 308 nm, were used for excitation, and fluorescence was collected at 420 nm. The excitation spot diameter was approximately 20 µm, and the sampling interval 0.15 mm. Within an area of 5*5 mm2, 1023 sampling points were recorded to generate 3D fluorescence maps of paper surfaces. Papers containing fluorescence whitening agents (FWAs) gave the highest average fluorescence signals. Coated papers with no FW As show weaker signals than the base sheet. For some thirty different paper samples, an obvious correlation between the amount of coating and the average intensity of the fluorescence signal was observed. Signal fluctuations around the average intensity values were sensitive to (1) the chemical pulp content in super calantered (SC) paper, (2) the amount of recycled fiber in newsprint, and (3) the amount of coating on the light-weight coated (LWC) paper surface. An effort was made to correlate fluorescence imaging results to predict mottling (diffusion of printing ink after printing) in various paper brands.
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9

Schneckenburger, Herbert. "Lasers in Live Cell Microscopy." International Journal of Molecular Sciences 23, no. 9 (April 30, 2022): 5015. http://dx.doi.org/10.3390/ijms23095015.

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Due to their unique properties—coherent radiation, diffraction limited focusing, low spectral bandwidth and in many cases short light pulses—lasers play an increasing role in live cell microscopy. Lasers are indispensable tools in 3D microscopy, e.g., confocal, light sheet or total internal reflection microscopy, as well as in super-resolution microscopy using wide-field or confocal methods. Further techniques, e.g., spectral imaging or fluorescence lifetime imaging (FLIM) often depend on the well-defined spectral or temporal properties of lasers. Furthermore, laser microbeams are used increasingly for optical tweezers or micromanipulation of cells. Three exemplary laser applications in live cell biology are outlined. They include fluorescence diagnosis, in particular in combination with Förster Resonance Energy Transfer (FRET), photodynamic therapy as well as laser-assisted optoporation, and demonstrate the potential of lasers in cell biology and—more generally—in biomedicine.
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10

Leppert, Jan, Jochen Krajewski, Sven Rainer Kantelhardt, Sven Schlaffer, Nadine Petkus, Erich Reusche, Gerion Hüttmann, and Alf Giese. "Multiphoton Excitation of Autofluorescence for Microscopy of Glioma Tissue." Neurosurgery 58, no. 4 (April 1, 2006): 759–67. http://dx.doi.org/10.1227/01.neu.0000204885.45644.22.

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Abstract OBJECTIVE: Intraoperative detection of residual tumor tissue in glioma surgery remains an important challenge because the extent of tumor removal is related to the prognosis of the disease. Multiphoton excited fluorescence tomography of living tissues provides high-resolution structural and photochemical imaging at a subcellular level. In this conceptual study, we have used multiphoton microscopy and fluorescence lifetime imaging (4D microscopy) to image cultured glioma cell lines, solid tumor, and invasive tumor cells in an experimental mouse glioma model and human glioma biopsy specimens. MATERIAL AND METHODS: A laser imaging system containing a mode-locked 80 MHz titanium:sapphire laser with a tuning range of 710 to 920 nm, a scan unit, and a time correlated single photon counting board was used to generate autofluorescence intensity images and fluorescence lifetime images of cultured cell lines, experimental intracranial gliomas in mouse brain, and biopsies of human gliomas. RESULTS: Multiphoton microscopy of native tumor bearing brain provided structural images of the normal brain anatomy at a subcellular resolution. Solid tumor, the tumor-brain interface, and single invasive tumor cells could be visualized. Fluorescence lifetime imaging demonstrated significantly different decay of the fluorescent signal in tumor versus normal brain, allowing a clear definition of the tumor-brain interface based on this parameter. Distinct fluorescence lifetimes of endogenous fluorophores were found in different cellular compartments in cultured glioma cells. The analysis of the relationship between the laser excitation wavelength and the lifetime of excitable fluorophores demonstrated distinct profiles for cells of different histotypes. CONCLUSION: Multiphoton excited fluorescence of endogenous fluorophores allows structural imaging of tumor and central nervous system histo-architecture at a subcellular level. The analysis of the decay of the fluorescent signal within specific excitation volumes by fluorescent lifetime imaging discriminates glioma cells and normal brain, and the excitation/lifetime profiles may further allow differentiation of cellular histotypes. This technology provides a noninvasive optical tissue analysis that may potentially be applied to an intraoperative analysis of resection plains in tumor surgery.
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Дисертації з теми "Laser Fluorescence Imaging"

1

Tecu, Kirk S. "Laser induced fluorescence imaging of counterflow diffusion flames /." free to MU campus, to others for purchase, 1997. http://wwwlib.umi.com/cr/mo/fullcit?p9841342.

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2

Sasaki, K., S. Matsui, H. Ito, and K. Kadota. "Dynamics of laser-ablation Ti plasmas studied by laser-induced fluorescence imaging spectroscopy." American Institute of Physics, 2002. http://hdl.handle.net/2237/7045.

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3

Capewell, Dale L. Goodwin David G. Goodwin David G. "Planar laser induced fluorescence imaging and Monte Carlo simulations of pulsed laser ablation /." Diss., Pasadena, Calif. : California Institute of Technology, 1997. http://resolver.caltech.edu/CaltechETD:etd-01102008-095243.

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4

Ombinda-Lemboumba, Saturnin. "Laser induced chlorphyll fluorescence of plant material." Thesis, Stellenbosch : University of Stellenbosch, 2007. http://hdl.handle.net/10019.1/3064.

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Thesis (MSc (Physics))--University of Stellenbosch, 2007.
Imaging and spectroscopy of laser induced chlorophyll fluorescence (LICF) are emerging as useful tools in plant physiology and agriculture since these methods allow an early detection of plant stress and transformation of plant tissue, before visual symptoms appear. Chlorophyll fluorescence is governed by photosynthetic efficiency and it depends on the plant species and physiological state. In addition, the laser induced fluorescence of chlorophyll molecules in the red and far red spectral range is also used to study basic processes and phenomena in photo-excited molecules. In the work reported here experimental setups used for laser induced chlorophyll fluorescence imaging and spectroscopy techniques were developed to investigate chlorophyll fluorescence under constant illumination and also to detect green-fluorescent protein (GFP) by looking at the chlorophyll fluorescence spectrum and image. He-Ne (wavelength 632 nm), tunable argon ion (wavelength 455 nm), and excimer (wavelength 308 nm) lasers were used as excitation sources. An Ocean Optics spectrometer was used to record the spectrum of the chlorophyll fluorescence and the variation of the chlorophyll fluorescence spectrum with time. The chlorophyll fluorescence spectrum of tobacco leaves expressing GFP was compared to that of control leaves. A charge-coupled device (CCD) camera was used to image the fluorescence from GFP expressing and control tobacco leaves to investigate the effect of GFP genes on chlorophyll fluorescence in relation to the state of the plant material. The spectral analysis technique and image processing procedures were elaborated in order to obtain better information on chlorophyll fluorescence. The results of this work show that the experimental setups and analytical procedures that were devised and used are suitable for laser induced chlorophyll fluorescence analysis. Fluorescence bleaching could be obtained from the time variation of the fluorescence spectrum, and plant expressing GFP can be distinguished from control plants by differences in the laser induced chlorophyll fluorescence.
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5

Jiang, Naibo. "Development of high repetition rate no planar laser induced fluorescence imaging." The Ohio State University, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=osu1150140816.

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6

Elson, Daniel S. "Development of ultrafast laser technology and its application to fluorescence lifetime imaging." Thesis, Imperial College London, 2003. http://hdl.handle.net/10044/1/12005.

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7

Auksorius, Egidijus. "Multidimensional fluorescence imaging and super-resolution exploiting ultrafast laser and supercontinuum technology." Thesis, Imperial College London, 2009. http://hdl.handle.net/10044/1/4201.

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This thesis centres on the development of multidimensional fluorescence imaging tools, with a particular emphasis on fluorescence lifetime imaging (FLIM) microscopy for application to biological research. The key aspects of this thesis are the development and application of tunable supercontinuum excitation sources based on supercontinuum generation in microstructured optical fibres and the development of stimulated emission depletion (STED) microscope capable of fluorescence lifetime imaging beyond the diffraction limit. The utility of FLIM for biological research is illustrated by examples of experimental studies of the molecular structure of sarcomeres in muscle fibres and of signalling at the immune synapse. The application of microstructured optical fibre to provide tunable supercontinuum excitation source for a range of FLIM microscopes is presented, including wide-field, Nipkow disk confocal and hyper-spectral line scanning FLIM microscopes. For the latter, a detailed description is provided of the supercontinuum source and semi-confocal line-scanning microscope configuration that realised multidimensional fluorescence imaging, resolving fluorescence images with respect to excitation and emission wavelength, fluorescence lifetime and three spatial dimensions. This included the first biological application of a fibre laser-pumped supercontinuum exploiting a tapered microstructured optical fibre that was able to generate a spectrally broad output extending to ~ 350 nm in the ultraviolet. The application of supercontinuum generation to the first super-resolved FLIM microscope is then described. This novel microscope exploited the concept of STED with a femtosecond mode-locked Ti:Sapphire laser providing a tunable excitation beam by pumping microstructured optical fibre for supercontinuum generation and directly providing the (longer wavelength) STED beam. This STED microscope was implemented in a commercial scanning confocal microscope to provide compatibility with standard biological imaging, and exploited digital holography using a spatial light modulator (SLM) to provide the appropriate phase manipulation for shaping the STED beam profile and to compensate for aberrations. The STED microscope was shown to be capable of recording super resolution in both the lateral and axial planes, according to the settings of the SLM.
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8

Lanigan, Peter Michael Pinto. "Applications of confocal and multiphoton laser scanning microscopes to multi-dimensional fluorescence imaging." Thesis, Imperial College London, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.439847.

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9

Sasaki, K., T. Wakasaki, S. Matsui, and K. Kadota. "Distributions of C_2 and C_3 radical densities in laser-ablation carbon plumes measured by laser-induced fluorescence imaging spectroscopy." American Institute of Physics, 2002. http://hdl.handle.net/2237/7043.

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10

Berckmuller, Martin. "A study of mixture formation in a lean burn research engine using laser fluorescence imaging." Thesis, Cranfield University, 1996. http://dspace.lib.cranfield.ac.uk/handle/1826/9933.

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Lean burn in spark-ignition engines offers a significant efficiency advantage compared with stoichiometric operation. The lean operation is restricted by increasing cyclic fluctuation in torque. In order to make use of the efficiency advantage and meet the mandatory emission standards the lean operation limit has to be further extended. This requires particular control of the mixing of fuel and air. To study the effect of mixture formation on cyclic variability and to provide quantitative information on the mixing of air and fuel planar laser-induced fluorescence (PLIF) was developed and applied to an operating SI engine. The method is based on imaging the fluorescence of a fluorescent marker (3-pentanone) mixed with the fuel (iso-octane). 3-pentanone was found to have similar vaporisation characteristics to those of iso-octane as well as low absorption and suitable spectral properties. The technique was applied to an one-cylinder SI engine with a cylinder head configuration based on the Honda VTEC-E lean burn system. The mixture formation process during the inlet and compression stroke could be described by measuring the average fuel concentration in four planes, between 0.7 and 15.2 mm below the spark plug, in a section of the cylinder orthogonal to the cylinder axis. The results showed that for 4-valve pent-roof cylinder head systems with swirl inlet flows, fuel impinging on the cylinder wall opposite to the inlet valves has a major influence on the mixture formation process. In order to quantify the cyclic variability in the mixture formation process and its contribution to cyclic variability in combustion the fuel concentration in a plane near the spark plug was measured on a large number of cycles. It could be shown, that the fuel concentration in a small region close to the spark plug has a dominating effect on the subsequent pressure development for lean mixtures. Variations in the mixture concentration in the vicinity of the spark plug contribute significantly to cyclic variations in combustion. In order to address the issue of nonuniformity in residual gas concentration prior to ignition a laser induced fluorescence method was developed to measure nitric oxide (NO) concentrations in the unburned charge in the same one-cylinder research engine. Measurements of average and instantaneous NO concentrations revealed, that the residual gas is not homogeneously mixed with the air and that significant cyclic variations in the local residual gas concentration exist.
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Книги з теми "Laser Fluorescence Imaging"

1

Paul, P. H. Applications of planar laser-induced fluorescence imaging diagnostics to reacting flows. Washington, D. C: American Institute of Aeronautics and Astronautics, 1990.

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2

Karsten, König, Tanke H. J, Schneckenburger Herbert, Society of Photo-optical Instrumentation Engineers., European Optical Society, European Laser Association, and Netherlands Medical Laser Association, eds. Laser microscopy: 7-8 July 2000, Amsterdam, Netherlands. Bellingham, Wash., USA: SPIE, 2000.

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3

Brandt, Roland, and Lidia Bakota. Laser scanning microscopy and quantitative image analysis of neuronal tissue. New York: Humana Press, 2014.

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4

R, Hicks Y., and United States. National Aeronautics and Space Administration., eds. Imaging fluorescent combustion species in gas turbine flame tubes: On complexities in real systems. [Washington, DC]: National Aeronautics and Space Administration, 1997.

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5

United States. National Aeronautics and Space Administration., ed. Quantitative PLIF imaging in high-pressure combustion: Final technical report for the period June 11, 1990 to September 20, 1996. Stanford, CA: High Temperature Gasdynamics Laboratory, Mechanical Engineering Department, Stanford University, 1997.

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6

Rinaldo, Cubeddu, Commission of the European Communities. Directorate-General for Science, Research, and Development., and Society of Photo-optical Instrumentation Engineers., eds. Proceedings of optical biopsy and fluorescence spectroscopy and imaging: 9-10 September 1994, Lille, France. Bellingham, Wash., USA: SPIE, 1994.

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7

R, Lakowicz Joseph, and Geddes Chris D, eds. Topics in fluorescence spectroscopy. New York: Plenum Press, 1991.

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8

Basché, T. Single-molecule optical detection, imaging and spectroscopy. Weinheim: VCH, 1997.

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9

1941-, Alfano Robert R., ed. Advances in optical biopsy and optical mammography. New York: New York Academy of Sciences, 1998.

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10

Alfano, Robert R., and Stavros G. Demos. Optical biopsy IX: 24-26 January 2011, San Francisco, California, United States. Bellingham, Wash: SPIE, 2011.

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Частини книг з теми "Laser Fluorescence Imaging"

1

Kohl, M., J. Neukammer, U. Sukowski, H. Rinneberg, H. J. Sinn, E. A. Friedrich, G. Graschew, P. Schlag, and D. Wohrle. "In Vitro Imaging of Tumors by Delayed Fluorescence." In Laser in der Medizin / Laser in Medicine, 312–16. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-50234-7_77.

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2

Schneckenburger, H., I. Tregub, R. Sailer, A. Rück, and W. S. L. Strauß. "Time-Resolved Fluorescence Spectroscopy and Imaging of Porphyrins." In Laser in der Medizin / Laser in Medicine, 627–30. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-80264-5_146.

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3

Clegg, R. M., P. C. Schneider, and T. M. Jovin. "Fluorescence Lifetime-Resolved Imaging Microscopy (FLIM)." In Biomedical Optical Instrumentation and Laser-Assisted Biotechnology, 143–56. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-009-1750-7_12.

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4

Kaneko, Junichi, Yoshinori Inagaki, Takeaki Ishizawa, and Norihiro Kokudo. "Near-Infrared Laser Photodynamic Therapy for Human Hepatocellular Carcinoma Cell Line Tumor with Indocyanine Green Fluorescence." In Fluorescence Imaging for Surgeons, 185–93. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-15678-1_19.

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5

Nakhosteen, J. A., and B. Khanavkar. "Autofluorescence Bronchoscopy: The Laser Imaging Fluorescence Endoscope." In Interventional Bronchoscopy, 236–42. Basel: KARGER, 1999. http://dx.doi.org/10.1159/000062106.

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6

König, Karsten. "Cellular Response to Laser Radiation in Fluorescence Microscopes." In Methods in Cellular Imaging, 236–51. New York, NY: Springer New York, 2001. http://dx.doi.org/10.1007/978-1-4614-7513-2_14.

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7

König, K., P. Fergin, M. W. Berns, and B. J. Tromberg. "Rapid Spectrally-Resolved Fluorescence Imaging of Skin After Topical ALA-Administration." In Laser in der Medizin / Laser in Medicine, 587–90. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-80264-5_138.

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8

Wells, K. Sam, David R. Sandison, James Strickler, and Watt W. Webb. "Quantitative Fluorescence Imaging with Laser Scanning Confocal Microscopy." In Handbook of Biological Confocal Microscopy, 27–39. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4615-7133-9_3.

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9

Leitz, Guenther, Armen Kurkdjian, Pierre Manigault, Abdellah Harim, and Karl Otto Greulich. "Laser Microperforation of Medicago Sativa Root Hair Cells." In Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence, 197–205. Boston, MA: Springer US, 1993. http://dx.doi.org/10.1007/978-1-4615-2828-9_22.

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10

Ebert, Bernd, and Dirk Grosenick. "Optical Imaging of Breast Tumors and of Gastrointestinal Cancer by Laser-Induced Fluorescence." In Molecular Imaging in Oncology, 331–50. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-10853-2_11.

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Тези доповідей конференцій з теми "Laser Fluorescence Imaging"

1

Kawata, Satoshi, and Rieko Arimoto. "Laser-scan fluorescence microscope with annular excitation optics." In OSA Annual Meeting. Washington, D.C.: Optica Publishing Group, 1990. http://dx.doi.org/10.1364/oam.1990.mpp1.

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We discuss the three-dimensional imaging characteristics of a laser-scan fluorescence microscope with an annular pupil in the excitation optics. As is well known, the use of an annular pupil in a conventional incoherent imaging system increases the depth of focus and the lateral resolution as compared with the use of a circular pupil.1,2 However, an annular pupil has not been practically used for fluorescence microscopy because it stops a large amount of the fluorescent light arriving at the objective lens. In the case of a laser-scan fluorescence microscope, we may place an annular pupil in the excitation optics rather than in the imaging optics. In this case, we do not waste fluorescent light because the objective-lens pupil is fully open.
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2

Cubeddu, R., A. Pifferi, P. Taroni, G. Valentini, G. Canti, C. Lindquist, S. Andersson-Engels, S. Svanberg, I. Wang, and K. Svanberg. "Advanced Laser Imaging Techniques in Medical Diagnosis." In The European Conference on Lasers and Electro-Optics. Washington, D.C.: Optica Publishing Group, 1998. http://dx.doi.org/10.1364/cleo_europe.1998.ctue1.

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Fluorescence images, based on the emission of endogenous or exogenous fluorophores, are being investigated for cancer diagnosis. Several photosensitizers, presently studied for the Photodynamic therapy of tumors, localize in neoplastic tissues more than in healthy surrounding ones and are fluorescent. Therefore, they are also suitable markers for diagnosis. A non-invasive diagnostic procedure calls for sensitizer doses much lower than the therapeutic ones. In such a condition, the exogenous fluorescence is very faint, and can be overcome by the natural tissue fluorescence. The enhancement of the signal to noise ratio can be achieved using either spectral filters (multicolor imaging) or a time gated acquisition. Multicolor imaging is based on the acquisition of three spectrally different images, one in the red region, which mainly collects the exogenous signal, one in the green-yellow region, which accounts for natural fluorescence, and one in the blue region, for normalization purposes. A pseudo-image is then calculated using a suitable mathematical algorithm.
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3

Yazdanfar, Siavash, Stephen A. Latham, Deborah S. Lee, Carl S. Lester, Robert J. Filkins, Stephen J. Lomnes, and John V. Frangioni. "Intraoperative near-infrared fluorescence imaging." In 2007 Quantum Electronics and Laser Science Conference. IEEE, 2007. http://dx.doi.org/10.1109/qels.2007.4431039.

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4

Yeung, Edward S., Wei Tong, and Sheri Lillard. "Cell Imaging by Laser-Induced Native Fluorescence Microscopy." In Laser Applications to Chemical and Environmental Analysis. Washington, D.C.: Optica Publishing Group, 1998. http://dx.doi.org/10.1364/lacea.1998.lma.2.

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The high degree of heterogeneity of the nervous and endocrine systems makes it extremely important for real-time monitoring of dynamic chemical changes at the single-cell level to gain a better understanding of the interaction of cells with their environment. Secretion mediated by exocytosis is one of the fundamental phenomena whose mechanism mimics the release of neurotransmitters at synaptic sites. Although the regulation of the secretory pathway has been studied extensively, its molecular mechanism is still not clear. It is important to develop methods that can follow real-time secretory processes with both high temporal and high spatial resolution. The native fluorescence of some proteins and neurotransmitters excited by a deep-UV laser has been shown to be a powerful probe for single-cell analysis. The advantages of direct native fluorescence detection include: (1) no chemical derivatization with fluorescent dyes is needed so no contamination or additional background will be introduced; (2) uncertainties about the efficiencies of the derivatization reaction are eliminated to ensure fast and quantitative response, without influences from slow reaction kinetics or incomplete equilibrium; and (3) the biological integrity of the cells will not be unnecessarily disturbed by having additional reagents or from exposure to artificial environments. We report the coupling of laser-induced native fluorescence detection with capillary electrophoresis (CE) to quantitatively monitor the secretion of insulin, serotonin and catecholamines from single cells. The uptake of serotonin by single living astrocytes was also recorded by native fluorescence imaging microscopy. The catecholamine (mainly epinephrine and norepinephrine) secreting adrenal chromaffin cells have been used as “model nerve terminals” to elucidate the molecular mechanism of neurotransmitter secretion at the nerve terminal. The in vitro dynamics of catecholamine release from bovine adrenal chromaffin cells was monitored with both high spatial and high temporal resolution.
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5

Thompson, Jill C. "Ultraviolet-laser-induced fluorescence imaging sensor." In Orlando '91, Orlando, FL, edited by Sankaran Gowrinathan, Raymond J. Mataloni, Sr., and Stanley J. Schwartz. SPIE, 1991. http://dx.doi.org/10.1117/12.44549.

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6

Hilton, Peter J. "Laser-induced fluorescence imaging of bacteria." In 1998 International Conference on Applications of Photonic Technology, edited by George A. Lampropoulos and Roger A. Lessard. SPIE, 1998. http://dx.doi.org/10.1117/12.328703.

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7

Harris, T. D., J. J. Macklin, J. K. Trautman, and L. E. Brus. "Imaging and Time-Resolved Spectroscopy of Single Molecules." In Laser Applications to Chemical and Environmental Analysis. Washington, D.C.: Optica Publishing Group, 1996. http://dx.doi.org/10.1364/lacea.1996.lwd.5.

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Recent progress in the fluorescence detection of individual molecules [1-8] suggests that a single dye molecule can be a useful tool to probe chemical identity and activity. Measurement of fluorescence lifetime [5,6] and spectrum [6] can be augmented by knowledge of molecular orientation using polarized light [3], and triplet [2] and photoisomer excitation, as well as diffusion processes, via fluorescence-intensity correlation. Applications of fluorescent probes include the study of the dynamic conformation of membrane-bound proteins, transport of and signaling by messenger molecules, and the optical detection of the sequence of DNA. While molecules can be spatially located using near-field microscopy [5-8], near-field probes can perturb the molecule under study. We show here that molecular properties can be determined easily and in a non-perturbative manner using far-field illumination, and we obtain unperturbed spectral and lifetime data that cannot be extracted from an ensemble measurement.
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8

Andersson, S., S. Montan, S. Svanberg, J. Ankerst, E. Kjellen, and K. Svanberg. "Tissue diagnostics using laser-induced fluorescence techniques." In International Laser Science Conference. Washington, D.C.: Optica Publishing Group, 1986. http://dx.doi.org/10.1364/ils.1986.fe2.

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The fluorescence emission from tissue subject to UV light excitation can be utilized for diagnostic purposes. The discrimination between tumors and normal tissue is of particular interest. The natural tissue fluorescence can be used but improved results are obtained using the agent hematoporphyrin derivative that is selectively retained in tumors. We have used laser-induced fluorescence for point measurements1 as well as for multicolor imaging2 of different kinds of rat tissue. For optimized characterization it is important to utilize the full spectral information and to form dimensionless contrast functions of measured spectral intensities. The importance of selecting the proper excitation wavelength is emphasized. The development of clinical instrumentation for point measurements and imaging is discussed.
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9

Sandwall, Peter, Henry Spitz, Howard Elson, Michael Lamba, William Connick, and Henry Fenichel. "Radio-fluorogenic dosimetry with violet diode laser-induced fluorescence." In SPIE Medical Imaging, edited by Bruce R. Whiting and Christoph Hoeschen. SPIE, 2014. http://dx.doi.org/10.1117/12.2043794.

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10

Lanigan, P. M. P., C. Dunsby, J. McGinty, D. S. Elson, J. Requejo-lsidro, I. Munro, N. Galletly, et al. "An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy." In 2005 Conference on Lasers and Electro-Optics (CLEO). IEEE, 2005. http://dx.doi.org/10.1109/cleo.2005.202127.

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Звіти організацій з теми "Laser Fluorescence Imaging"

1

Strand, Michael P. Coastal Benthic Optical Properties Fluorescence Imaging Laser Line Scan Sensor. Fort Belvoir, VA: Defense Technical Information Center, September 2002. http://dx.doi.org/10.21236/ada628584.

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2

Chyu, M. K. Use of a laser-induced fluorescence thermal imaging system for film cooling heat transfer measurement. Office of Scientific and Technical Information (OSTI), April 1996. http://dx.doi.org/10.2172/226040.

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3

Franks, Peter J., and Jules S. Jaffe. Planar Laser Imaging of Scattering and Fluorescence of Zooplankton Feeding in Layers of Phytoplankton in situ. Fort Belvoir, VA: Defense Technical Information Center, January 2006. http://dx.doi.org/10.21236/ada521889.

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4

Franks, Peter J., and Jules S. Jaffe. Planar Laser Imaging of Scattering and Fluorescence of Zooplankton Feeding in Layers of Phytoplankton in situ. Fort Belvoir, VA: Defense Technical Information Center, September 2007. http://dx.doi.org/10.21236/ada574182.

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5

Airborne laser induced fluorescence imaging. Innovative technology summary report. Office of Scientific and Technical Information (OSTI), June 1999. http://dx.doi.org/10.2172/354882.

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