Готові списки джерел за темами / Glycosides Analysis

Добірка наукової літератури з теми "Glycosides Analysis"

Автор: Grafiati

Опубліковано: 4 червня 2021

Оновлено: 31 січня 2023

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Статті в журналах з теми "Glycosides Analysis":

Zheng, Yunfeng, Weiping Duan, Jie Sun, Chenguang Zhao, Qizhen Cheng, Cunyu Li, and Guoping Peng. "Structural Identification and Conversion Analysis of Malonyl Isoflavonoid Glycosides in Astragali Radix by HPLC Coupled with ESI-Q TOF/MS." Molecules 24, no. 21 (October 31, 2019): 3929. http://dx.doi.org/10.3390/molecules24213929.

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In this study, four malonyl isoflavonoid glycosides (MIGs), a type of isoflavonoid with poor structural stability, were efficiently isolated and purified from Astragali Radix by a medium pressure ODS C18 column chromatography. The structures of the four compounds were determined on the basis of NMR and literature analysis. Their major diagnostic fragment ions and fragmentation pathways were proposed in ESI/Q-TOF/MS positive mode. Using a target precursor ions scan, a total of 26 isoflavonoid compounds, including eleven malonyl isoflavonoid glycosides coupled with eight related isoflavonoid glycosides and seven aglycones were characterized from the methanolic extract of Astragali Radix. To clarify the relationship of MIGs and the ratio of transformation in Astragali Radix under different extraction conditions, two MIGs (calycosin-7-O-glycoside-6″-O-malonate and formononetin-7-O-glycoside-6″-O-malonate) coupled with related glycosides (calycosin-7-O-glycoside and formononetin-7-O-glycoside) and aglycones (calycosin and formononetin) were detected by a comprehensive HPLC-UV method. Results showed that MIGs could convert into related glycosides under elevated temperature conditions, which was further confirmed by the conversion experiment of MIGs reference compounds. Moreover, the total contents of MIGs and related glycosides displayed no obvious change during the long-duration extraction. These findings indicated that the quality of Astragali Radix could be evaluated efficiently and accurately by using the total content of MIGs and related glycosides as the quality index.

Caffrey, Andrew, and Susan E. Ebeler. "The Occurrence of Glycosylated Aroma Precursors in Vitis vinifera Fruit and Humulus lupulus Hop Cones and Their Roles in Wine and Beer Volatile Aroma Production." Foods 10, no. 5 (April 24, 2021): 935. http://dx.doi.org/10.3390/foods10050935.

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Volatile aroma compounds found in grapes and hops may be present as both free volatiles and bound glycosides. Glycosides found in the raw materials are transferred to their respective fermented beverages during production where the odorless compounds may act as a reservoir of free volatiles that may be perceived by the consumer if hydrolyzed. A review of the literature on grape and wine glycosides and the emerging literature for glycosides in hops is presented in order to demonstrate the depth of history in grape glycoside research and may help direct new research on hop glycosides. Focus is brought to the presence of glycosides in the raw materials, the effect that winemaking and brewing have on glycoside levels, and current methods for the analysis of glycosidically linked aroma compounds.

Peng, Wenwen, Xiaoxiang Fu, Yuyan Li, Zhonghua Xiong, Xugen Shi, Fang Zhang, Guanghua Huo, and Baotong Li. "Phytochemical Study of Stem and Leaf of Clausena lansium." Molecules 24, no. 17 (August 28, 2019): 3124. http://dx.doi.org/10.3390/molecules24173124.

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Clausena lansium Lour. Skeels (Rutaceae) is widely distributed in South China and has historically been used as a traditional medicine in local healthcare systems. Although the characteristic components (carbazole alkaloids and coumarins) of C. lansium have been found to possess a wide variety of biological activities, little attention has been paid toward the other components of this plant. In the current study, phytochemical analysis of isolates from a water-soluble stem and leaf extract of C. lansium led to the identification of 12 compounds, including five aromatic glycosides, four sesquiterpene glycosides, two dihydrofuranocoumarin glycosides, and one adenosine. All compounds were isolated for the first time from the genus Clausena, including a new aromatic glycoside (1), a new dihydrofuranocoumarin glycoside (6), and two new sesquiterpene glycosides (8 and 9). The phytochemical structures of the isolates were elucidated using spectroscopic analyses including NMR and MS. The existence of these compounds demonstrates the taxonomic significance of C. lansium in the genus Clausena and suggests that some glycosides from this plant probably play a role in the anticancer activity of C. lansium to some extent.

Miyagawa, Yasuyuki, Takahito Mizukami, Hiroshi Kamitakahara та Toshiyuki Takano. "Synthesis and fundamental HSQC NMR data of monolignol β-glycosides, dihydromonolignol β-glycosides and p-hydroxybenzaldehyde derivative β-glycosides for the analysis of phenyl glycoside type lignin-carbohydrate complexes (LCCs)". Holzforschung 68, № 7 (1 жовтня 2014): 747–60. http://dx.doi.org/10.1515/hf-2013-0164.

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Abstract Twelve monolignol (coniferyl alcohol, sinapyl alcohol and p-coumaryl alcohol) β-glycosides (β-glucosides, β-galactosides, β-xylosides and β-mannosides) were synthesised to obtain fundamental NMR data for the analysis of phenyl glycoside type lignin-carbohydrate complexes (LCCs). That is, the 1,2-trans glycosides (the β-glucosides, β-galactosides and β-xylosides) and the 1,2-cis glycosides (the β-mannosides) were synthesized by means of Koenig-Knorr glycosylation and β-selective Mitsunobu glycosylation strategies, respectively. In addition, dihydromonolignol and p-hydroxybenzaldehyde derivative β-glycosides were also prepared from the corresponding monolignol glycosides and their intermediates, respectively. The correlation observed for the C1β-H1β bonds of the sugar moieties in the HSQC spectra of the all β-glycosides varied and were in the range of δC/δH 96–104/4.7–5.4 ppm. Especially, it was found that the correlations derived from the C1β-H1β bonds of the guaiacyl and p-hydroxyphenyl β-mannosides were close to those derived from the C1α-H1α bonds of the 4-O-methyl-α-D-glucuronic acid moieties described in the literature.

Yokosuka, Akihito, and Yoshihiro Mimaki. "Steroidal Glycosides from the Underground Parts of Agapanthus inapertus and Their Cytotoxic Activity." Natural Product Communications 2, no. 1 (January 2007): 1934578X0700200. http://dx.doi.org/10.1177/1934578x0700200107.

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Phytochemical investigation of the underground parts of Agapanthus inapertus (Liliaceae) has resulted in the isolation of three new spirostanol glycosides (1, 3, and 4), along with a known spirostanol glycoside (2) and two known spirostanols (5 and 6). The structures of the new glycosides were determined by spectroscopic analysis and the results of hydrolytic cleavage. The isolated compounds were evaluated for their cytotoxic activity against HL-60 human promyelocytic leukemia cells.

Agzamova, Manzura Adkhamovna, Ravshanjon Muratjanovich Khalilov та Abdulaziz Adilkhanovich Janibekov. "СHROMATOGRAPHIC ANALYSIS OF СYCLOSIVERSIOSIDE F". chemistry of plant raw material, № 2 (10 червня 2021): 267–74. http://dx.doi.org/10.14258/jcprm.2021028314.

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The plants Astragalus pterocephalus growing in Uzbekistan are a source of triterpene glycosides. The main triterpene glycoside, in terms of content, is a cycloartan glycoside – cyclosiversioside F. To obtain an individual biologically active compound cyclosiversioside F with 95% purity, a proposed method involves extraction with methanol, concentration and dilution with an equal volume of water, then followed by a sequential extraction from the aqueous extract with chloroform, ethyl acetate and butanol. Then a chromatographic separation of the purified amount of extractives on a column with silica gel, isolation of the substance and precipitation from a solvent system must be performed, followed by recrystallization and drying. The optimal conditions for the isolation and separation of the amount of extractive substances have been developed in order to obtain an individual glycoside. Cyclosiversioside F was authenticated by TLC in comparison with an authentic sample. Quantitative analysis of the glycoside was carried out by HPLC. The purity of cyclosiversioside F was confirmed by taking 1H and 13C NMR spectra.

Deng, Xuming, Hu Shang, Jiajia Chen, Jun Wu, Tao Wang, Yiqing Wang, Chensong Zhu, and Weijiang Sun. "Metabolomics Combined with Proteomics Provide a Novel Interpretation of the Changes in Flavonoid Glycosides during White Tea Processing." Foods 11, no. 9 (April 24, 2022): 1226. http://dx.doi.org/10.3390/foods11091226.

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In this study, nonvolatile metabolomics and proteomics were applied to investigate the change mechanism of flavonoid glycoside compounds during withering processing of white tea. With the extension of withering time, the content of the main flavonoid glycoside compounds significantly decreased, and then the flavonoid aglycones and water-soluble saccharides contents increased. However, the change trends of these compounds were inconsistent with the expression pattern of related biosynthesis pathway proteins, indicating that the degradation of flavonoid glycosides might exist in the withering process of white tea. One co-expression network that was highly correlated with variations in the flavonoid glycosides’ component contents during the withering process was identified via WGCNA. Further analysis revealed that the degradation of flavonoid glycosides may be related to the antioxidant action of tea leaves undergoing the withering process. Our results provide a novel characterization of white tea taste formation during processing.

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Perrone, Angela, Milena Masullo, Alberto Plaza, Arafa Hamed, and Sonia Piacente. "Flavone and Flavonol Glycosides from Astragalus eremophilus and Astragalus Vogelii." Natural Product Communications 4, no. 1 (January 2009): 1934578X0900400. http://dx.doi.org/10.1177/1934578x0900400117.

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Two new rhamnocitrin glycosides (1 and 2) were isolated from the aerial parts of Astragalus vogelii, along with one known rhamnocitrin glycoside (3). Two known flavonol glycosides (4 and 5) and four known flavone derivatives (6-9) were isolated from the aerial parts of Astragalus eremophilus. Their structures were elucidated by extensive spectroscopic methods including 1D- (1H, 13C and TOCSY) and 2D-NMR (DQF-COSY, HSQC, HMBC) experiments, as well as ESIMS analysis.

Kırmızıbekmez, Hasan, Carla Bassarello, Sonia Piacente, Galip Akaydın, and İhsan Çalış. "Flavonoid, Phenylethanoid and Iridoid Glycosides from Globularia aphyllanthes." Zeitschrift für Naturforschung B 64, no. 2 (February 1, 2009): 252–56. http://dx.doi.org/10.1515/znb-2009-0217.

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A new flavone glycoside, 6-hydroxyluteolin 7-O-[6m-benzoyl-β -D-glucopyranosyl-(1 → 2)]-β - D-glucopyranoside (aphyllanthoside, 1) was isolated from the MeOH extract of the aerial parts of Globularia aphyllanthes. Besides this new compound, two flavonoid glycosides (6-hydroxyluteolin 7-O-[6m-(E)-caffeoyl-β -D-glucopyranosyl-(1 → 2)]-β -D-glucopyranoside and isoquercitrin), three phenylethanoid glycosides (verbascoside, rossicaside A, and trichosanthoside A), and 11 iridoid glycosides (aucubin, catalpol, 10-O-benzoylcatalpol, globularin, asperuloside, besperuloside, asperulosidic acid, daphylloside, scandoside, alpinoside and baldaccioside) were also obtained and characterized. Identification of the isolated compounds was carried out by spectroscopic analysis including 1D and 2D NMR experiments as well as HRMS

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Дисертації з теми "Glycosides Analysis":

Watermeyer, Jean Margaret. "Human testis angiotensin-converting enzyme: Crystal structure of a glycosylation mutant and investigation of a putative hinge-mechanism by normal mode analysis." Thesis, University of the Western Cape, 2004. http://etd.uwc.ac.za/index.php?module=etd&amp.

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Human angiotensin-converting enzyme (ACE) is a key enzyme in the regulation of blood pressure via the renin-angiotensin and kallikrein-kinin systems. A number of orally active drugs have been developed over the years that target somatic ACE, for the treatment of hypertension, myocardial infarction and congestive heart failure. Protein structural information about ACE is an important key for the understanding of the mechanism and substrate-specificity of the enzyme. However, this information has only begun to be elucidated in the past year, with the solution of crystal structures of human testis ACE (tACE), and homologues Drosophila AnCE and human ACE2. tACE is identical to the C-terminal domain of somatic ACE, which consists of two homologous domains, each having a slightly different substrate-specificity. This thesis describes the purification, crystallisation and X-ray crystal structure-determination of a glycosylation-deficient mutant of tACE, tACEG1,3, to 2.9 Å
.

Iland, Patrick. "A study of Glycosides in grapes and wines of Vitis vinifera cv. Shiraz." Title page, contents and summary only, 2001. http://web4.library.adelaide.edu.au/theses/09PH/09phI27.pdf.

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Includes a list of publications co-authored by the author during the preparation of this thesis. Bibliography: leaves 103-111. Studies the links between grape composition, wine composition and wine sensory properties. Developed a new method of measuring glycoside concentration in grapes (glycosyl-glucose assay) and a modified measurement of wine colour density in red wine. These were used to analyse samples of Shiraz grapes and wines from a comprehensive vineyard irrigation trial. Glycosyl-glucose concentrations shows promise for the prediction of wine composition and flavor intensity.

Ackloo, Suzanne. "Structural analysis of ginsenosides and sugars : an electrospray and tandem mass spectrometry study /." *McMaster only, 2001.

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Brimer, Leon. "Cyanogenic glycosides : occurence, analysis and removal from food and feed ; comparison to other classes of toxic and antinutritional glycosides ; technology and biotechnology for the removal of plant toxins /." Copenhagen : Department of Pharmacology and Pathobiology, Laboratory of Toxicology, Royal Veterinary and Agricultural Univ, 2000. http://www.gbv.de/dms/bs/toc/319505995.pdf.

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Wolleben, Charles Daniel. "An Analysis of the Reversible Phosphorylation of Glycogen Synthase in Rat Heart: a Dissertation." eScholarship@UMMS, 1986. http://escholarship.umassmed.edu/gsbs_diss/288.

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The aim of this study has been to explore the site specific phosphorylation pattern of rat heart glycogen synthase paying particular attention to phosphorylations that are important to the in vivo control of enzyme activity. This problem has been approached using techniques of immuneprecipitation of 32P labeled synthase from hormonally responsive, freshly isolated adult rat cardiomyocytes. Identification of the active subunit of rat heart glycogen synthase was accomplished by immuneprecipitating synthase from 32P-labeled cardiomyocytes and performing Western blot analysis on DEAE-cellulose fractions containing synthase activity. Using these methods, glycogen synthase activity has been localized to a protein of 88,000 daltons. Reverse phase HPLC analysis of synthase tryptic peptides from either hormone responsive cardiomyocytes or synthase treated in vitro with cAMP-dependent protein kinase and protein phosphatase-1 (PP-1) resulted in finding six reproducible peaks of phosphopeptides. The incorporation of radioactivity into peaks 1 and 2 was associated with both the treatment of cardiomyocytes with epinephrine and the in vitro phosphorylation of rat heart synthase with cAMP-dependent protein kinase. These same two peaks are selectively dephosphorylated when cAMP-dependent kinase treated synthase is incubated with protein phosphatase-1. This dephosphorylation of peaks 1 and 2 are coincident with the conversion of synthase from the D to the I form. Peak 3 is dephosphorylated upon treatment of cardiomyocytes with insulin and hyperphosphorylated in cardiomyocytes derived from alloxan diabetic animals. Taken together these results demonstrates the direct relationship between the phosphopeptides in peaks 1 and 2 and the inhibition of synthase activity in response to epinephrine treatment in the cell. This inhibition can be explained by the activity of cAMP-dependent protein kinase which can duplicate the intracellular, epinephrine-stimulated synthase phosphopeptide pattern. This inhibition can be relieved in vitro by protein phosphatase-1 which dephosphorylates peaks 1 and 2. The effect of insulin and alloxan diabetes is localized to peak 3 whose phosphorylation is unaffected in vitro by either cAMP-dependent protein kinase or protein phosphatase-1.

Nguyen, Phuc Dam. "Etude phytochimique de plantes de la médecine traditionnelle du Vietnam et du Laos. Evaluation biologique dans le domaine de la santé." Thesis, Reims, 2015. http://www.theses.fr/2015REIMP204/document.

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L’objectif de ce travail est de contribuer à l'amélioration des connaissances phytochimiques et biologiques des plantes medicinales, afin d’en valoriser et d’en promouvoir l'usage en médicine traditionnelle au Vietnam. Dans ce travail de thèse, nous avons mené une étude phytochimique sur trois espèces végétales: Cleome chelidonii (Cleomaceae), Dolichandrone spathacea (Bignoniaceae) et Flacourtia rukam (Salicaceae). 90 composés ont été isolés et leurs structures ont été déterminées à l’aide des techniques spectroscopiques de RMN 1D et 2D, par la spectrométrie de masse ESI-MS, des données spectrales en UV, IR, la mesure du pouvoir rotatoire et des courbes de CD, et par comparaison avec les données de la littérature. Parmi ces composés, 29 correspondent à des molécules nouvellement décrites. Les composés isolés peuvent être classés en plusieurs groupes : flavonoïdes, iridoides, saponosides, mégastigmanes, glycosides phénoliques, alcaloïdes, …L’évaluation de l’activité anti-radicalaire (test DPPH) a été effectuée sur les flavonoides de C. chelidonii, et les activités antimicrobiennes des extraits et composés de D. spathacea et F. rukam ont été mesurées. Parmi les composés testés, le glycoside de quercétol nouveau CF-3 est le seul à posséder une activité anti-radicalaire importante (CI50 = 17,74 µM) et le glucoside phénolique nouveau FRP-4 possède l’activité antibactérienne la plus importante contre trois bactéries à Gram positif (CMI = 31,2 µg/ml) et deux bactéries à Gram négatif (CMI = 125 µg/ml)
The objective of this work is to contribute to the improvement of phytochemical and biological knowledge of medicinal plants, in order to enhance and promote their uses in traditional medicine in Vietnam. In this thesis, we carried out a phytochemical study on three plants: Cleome chelidonii (Cleomaceae) Dolichandrone spathacea (Bignoniaceae) and Flacourtia rukam (Salicaceae). 90 compounds were isolated and their structures were determinated using the spectroscopic techniques of 1D & 2D NMR and by the ESI-MS mass spectrometry, spectral data UV, IR, measurement of optical rotation and CD, and by comparison with the literature data. Among them, 29 are new molecules. The isolated compounds may be classified into many groups: flavonoids, iridoids, saponins, megastigmanes, phenolic glycosides, alkaloids...The antiradical activity of the flavonoids of C. Chelidonii was evaluated by the DPPH test, and the antimicrobial activity were examinated on all extracts and compounds of D. spathacea and F. rukam. Among the tested compounds, the new flavonoid CF-3 has a significant anti-radical activity (IC50 = 17.74 µM) and the new phenolic glucoside FRP-4 has the most significant antibacterial activity against three Gram-positive bacteria (MIC = 31.2 µg / ml) and two gram-negative bacteria (MIC = 125 µg /ml)

Chiwona-Karltun, Linley. "A reason to be bitter : cassava classification from the farmers' perspective /." Stockholm : [Karolinska institutets bibl.], 2001. http://diss.kib.ki.se/2001/91-7349-078-4/.

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Street, Ian Philip. "Fluorinated carbohydrates as probes of mechanism and specificity in glycosyl transferases." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/29434.

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The Compounds 2-deoxy-2-fluoro-β-D-glucosyl fluoride (1), 2,4-dinitrophenyl 2-deoxy-2-fluoro-β-D-glucoside (2), 2-deoxy-2-fluoro-β-D-galactosyl fluoride (3) and 2-deoxy-2-fluoro-β-D-mannosyl fluoride (4) were all found to be potent covalent inhibitors of β-glucosidase from Alcaligenes faecalis (pABG5 β-glucosidase), which function through the accumulation of a stable glycosyl-enzyme intermediate. The mechanism of action for these inhibitors was investigated and found to be both specific and active site directed, involving a 1:1 stoichiometric formation of an enzyme inhibitor complex. Investigation of the pre-steady state kinetics for the inhibition reaction provided values for the rates of formation (kon) and hydrolysis (koff) of the glycosylated-enzyme intermediate. The values of kon determined in this manner are 5.9, 25, 3.6 and 5.6 min.⁻¹ for compounds 1,2,3 and 4 respectively, while the values of koff were found to be much smaller; 1.5x10⁻⁵, 5.4 x 10⁻³ and 1.0 x 10⁻³ min.⁻¹, respectively for the intermediates formed from compounds 1, 3 and 4. The isolated intermediates were also found to be competent in the catalysis of transglycosylation reactions and evidence for the participation of a specific binding site for the acceptor compound in this process was gained. The results of these kinetic experiments were corroborated by data obtained from ¹⁹F-NMR spectra of the glycosyl-enzyme intermediate and by isolation and subsequent characterization of the transglycosylation products. The stereochemistry of the inhibition reaction was investigated by a simple ¹⁹F-NMR experiment, and was found to be consistent with a double displacement reaction mechanism, as would be expected from the generally accepted reaction mechanism for this type of enzyme. The compound D-glucal was found to be a substrate for pABG5 β-glucosidase (kcat = 2.28 min.⁻¹ Km = 0.85 mM). Hydration of this compound by pABG5 β-glucosidase in deuterated buffers demonstrated that the double bond of D-glucal was deuterated stereo-specifically from below the α-face. The compound 2-fluoro-D-glucal was found to be a weak competitive inhibitor (Ki = 30 mM) of pABG5 β-glucosidase. Glycogen phosphorylase catalyses the reversible phosphorolysis of glycogen. A series of deoxy analogues of the enzymes natural substrate, α-D-glucose 1-phosphate have been prepared and along with a number of deoxyfluoro analogues tested as substrates. All were found to act as substrates but at exceedingly slow rates. The large rate reductions when compared with the normal substrate can be attributed to a deleterious combination of electronic and binding effects in the modified substrates reducing the stability of the enzymic transition states. A linear free energy relationship between kcat and the first order rate constant for the acid catalysed hydrolysis of the same series of deoxy and deoxyfluoro glucopyranosyl phosphates was demonstrated, suggesting similar transition states for the two reactions and implicating an oxocarbonium ion-like transition state in the enzymic reaction. The binding data obtained from the steady state kinetics of these analogues suggests that hydrogen bonding interactions are qualitatively conserved in the glucopyranose binding site during the T- to R-state conformational transition of the enzyme and that interactions between the enzyme and the hydroxyl groups at the 3- and 6-positions of the glucopyranose ring of the substrate are potentially important for stabilization of the enzymic transition state. The specificity of the substrate phosphate binding site has been probed using the compounds 2-deoxy-2-fluoro-α-D-glucopyranosyl phosphate (5), (1-deoxy-α-D-glucopyranosyl) methylphosphonate (6) and 2-deoxy-2-fluoro-α-D-glucopyranosyl phosphofluoridate (7). The results suggested that phosphorylase b can bind both the monanionic and dianionic forms of its substrate with approximately equal affinity. NMR studies of the ternary enzyme-ligand complexes formed with glycogen phosphorylase b and 5 or 6 indicated that no proton donation occurred in the ground-state active site complex. A preliminary investigation into the ability of the cellulase complex from a number of different wood-degrading fungi to hydrolyse p-nitrophenyl β-glucoside has been carried out. This work is aimed at producing environmentally safe fungicides, which are activated by the β-glucosidase component of the cellulase complex in these organisms and this study was carried out in conjunction with Forintek Canada.
Science, Faculty of
Chemistry, Department of
Graduate

Mosina, Leticia Leticia. "Structure-function analysis of a novel multi-functional glycoside hydrolase." Thesis, University of Pretoria, 2019. http://hdl.handle.net/2263/77859.

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The present study presents the research findings of the first ever multi-modular Paenibacillus mucilaginosus glycoside hydrolase (PmGH). Furthermore, we report the successful crystallisation of a multi-modular GH. The GH is composed of two catalytic modules (GH5 and GH6) and two carbohydrate binding modules (both CBM3). Functional analysis demonstrated that the cellulase, mannanase and xylanase activities of PmGH (130 kDa) were attributed to the GH5 catalytic domain. The presence of the GH6 catalytic domain resulted in slightly increased cellulase activity in PmGH. Optimal PmGH activity and functional stability was highest at pH 4-6 and at 40-60°C The structural properties of PmGH that determine its robust nature were further investigated. Homology modelling of PmGH showed the GH5 and GH6 domains to be independent but provided no structural information for the CBMs and linker regions. However, successful homology modelling of the individual domains indicated that the combination of the modules makes PmGH structurally and functionally novel. Glycoside hydrolases occur as independent modules or as part of a multi-modular protein with other catalytic and/or non-catalytic modules. Multiple combinations of these modules can occur in nature resulting in novel proteins such as PmGH. In an attempt to determine the PmGH crystal structure, a range of crystallisation conditions were tested. After extensive screening and optimisation, multiple PmGH crystals were diffracted, using both local diffraction and Synchrotron radiation sources (ESRF, France). Overall ~90% of the PmGH protein crystals did not diffract and of the remaining ~10% yielded unsatisfactory data. Phasing by molecular replacement also yielded no structural solutions. Alternative phasing methods such as multi-wavelength anomalous dispersion were also unsuccessful due to the quality of the diffraction data collected. Given the severe lack of multi-modular GH crystal structures in protein structure databases, the present study highlights the major limitations in structural studies of these important enzymes.
Thesis (PhD)--University of Pretoria, 2018.
Genetics
PhD
Unrestricted

Mertz, Blake. "Computational analysis of the phylogeny and thermodynamics of glycoside hydrolases." [Ames, Iowa : Iowa State University], 2008.

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Книги з теми "Glycosides Analysis":

Brimer, Leon. Cyanogenic glycosides: occurence, analysis and removal from food and feed: Comparison to other classes of toxic and antinutritional glycosides : technology and biotechnology for the removal of plant toxins. Copenhagen: Royal Veterinary and Agricultural University, 2000.

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Wagner, Hildebert. Plant drug analysis: A thin layer chromatography atlas. 2nd ed. Dordrecht: Springer, 2009.

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Wagner, Hildebert. Plant drug analysis: A thin layer chromatography atlas. 2nd ed. Berlin: Springer, 1996.

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Wagner, Hildebert. Plant drug analysis: A thin layer chromatography atlas. 2nd ed. Dordrecht: Springer, 2009.

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Wagner, Hildebert. Plant drug analysis: A thin layer chromatography atlas. 2nd ed. Dordrecht: Springer, 2009.

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Yukihiro, Shoyama, ed. Analysis of natural glycosides. Trivandrum: Research Signpost, 2007.

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Uddin, Ahmad Viqar, and Basha Anwer 1947-, eds. Spectroscopic data of steroid glycosides. New York: Springer, 2007.

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Ahmad, Viqar Uddin, and Anwer Basha. Spectroscopic Data of Steroid Glycosides. Springer, 2010.

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Spectroscopic data of steroid glycosides. New York: Springer, 2007.

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(Editor), Anwer Basha, and Viqar Uddin Ahmad (Editor), eds. Spectroscopic Data of Steroid Glycosides. Springer, 2007.

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Частини книг з теми "Glycosides Analysis":

Wölwer-Rieck, U. "CHAPTER 4. Analysis of Steviol Glycosides." In Food Chemistry, Function and Analysis, 84–112. Cambridge: Royal Society of Chemistry, 2018. http://dx.doi.org/10.1039/9781788010559-00084.

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Frentzen, M., P. Besrukow, A. Ackermann, S. Pierog, B. Schiermeyer, J. Winter, U. Wölwer-Rieck, and D. Kraus. "CHAPTER 9. Steviol Glycosides in Dentistry." In Food Chemistry, Function and Analysis, 162–84. Cambridge: Royal Society of Chemistry, 2018. http://dx.doi.org/10.1039/9781788010559-00162.

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Brinker, A. M., and D. S. Seigler. "Determination of Cyanide and Cyanogenic Glycosides from Plants." In Plant Toxin Analysis, 359–81. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-662-02783-7_15.

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Mensah, Michael Amoafo. "Cyanogenic Glycosides as Food Toxins." In Analysis of Naturally Occurring Food Toxins of Plant Origin, 25–52. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003222194-3.

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Perret, J. "CHAPTER 3. Steviol Glycosides Production: Traditional Versus New Technologies." In Food Chemistry, Function and Analysis, 59–83. Cambridge: Royal Society of Chemistry, 2018. http://dx.doi.org/10.1039/9781788010559-00059.

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Williams, Patrick J., and I. Leigh Francis. "Sensory Analysis and Quantitative Determination of Grape Glycosides." In ACS Symposium Series, 124–33. Washington, DC: American Chemical Society, 1996. http://dx.doi.org/10.1021/bk-1996-0637.ch012.

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Philippaert, Koenraad, Caroline Wuyts, Caroline Simoens, and Rudi Vennekens. "CHAPTER 10. Sensory Effects of Steviol Glycosides: Taste Perception and Beyond." In Food Chemistry, Function and Analysis, 185–203. Cambridge: Royal Society of Chemistry, 2018. http://dx.doi.org/10.1039/9781788010559-00185.

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Goekjian, Peter G., Alexander Wei, and Yoshito Kishi. "Conformational Analysis of C-Glycosides and Related Compounds: Programming Conformational Profiles of C- and O-Glycosides." In Carbohydrate-Based Drug Discovery, 305–40. Weinheim, FRG: Wiley-VCH Verlag GmbH & Co. KGaA, 2005. http://dx.doi.org/10.1002/3527602437.ch11.

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Oehme, A., W. Schwab, and M. Wüst. "CHAPTER 2. Biosynthesis of Steviol Glycosides and Related Diterpenes in Leaves and Glandular Trichomes of Stevia rebaudiana Bertoni." In Food Chemistry, Function and Analysis, 32–58. Cambridge: Royal Society of Chemistry, 2018. http://dx.doi.org/10.1039/9781788010559-00032.

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Testai, Lara, and Vincenzo Calderone. "CHAPTER 8. Stevia rebaudiana Bertoni: Beyond Its Use as a Sweetener. Pharmacological and Toxicological Profile of Steviol Glycosides of Stevia rebaudiana Bertoni." In Food Chemistry, Function and Analysis, 148–61. Cambridge: Royal Society of Chemistry, 2018. http://dx.doi.org/10.1039/9781788010559-00148.

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Тези доповідей конференцій з теми "Glycosides Analysis":

Kwon, Ha-Jeong, and Yong-Duk Park. "Analysis of glycosides in traditional chinese medicine using electrochemical detection." In 2010 3rd International Conference on Biomedical Engineering and Informatics (BMEI). IEEE, 2010. http://dx.doi.org/10.1109/bmei.2010.5640085.

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Kaushik, Vivek, Yogesh Kaulkarni, Clayton Wright, George O'Doherty, Neelam Azad, and Anand Iyer. "Abstract 3205: Structural analysis of cardiac glycosides to determine the basis for tumoristatic activity." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-3205.

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Martono, Yohanes, Sugeng Riyanto, Abdul Rohman, and Sudibyo Martono. "Improvement method of fast and isocratic RP-HPLC analysis of major diterpene glycoside from Stevia rebaudiana leaves." In PROCEEDINGS OF THE 12TH INTERNATIONAL CONFERENCE ON SYNCHROTRON RADIATION INSTRUMENTATION – SRI2015. Author(s), 2016. http://dx.doi.org/10.1063/1.4958509.

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Girme, A., A. Mirgal, and L. Hingorani. "Role of high-performance thin-layer chromatography method in separation and analysis of withanosides-withanolides with flavonoid glycoside in Withania somnifera." In GA – 70th Annual Meeting 2022. Georg Thieme Verlag KG, 2022. http://dx.doi.org/10.1055/s-0042-1759030.

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Звіти організацій з теми "Glycosides Analysis":

Tian, Huichuan, Jiajun Ren, and Meilan Zhang. Alprostadil Combined with Tripterygium Glycosides in the Treatment of Diabetic nephropathy：A Systematic Review and Meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, August 2020. http://dx.doi.org/10.37766/inplasy2020.8.0063.

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Li, Zifeng, Xiaolan Fu, Long Yin, Xiaoqiang Hou, and Caiyun Chang. Clinical effect of Tripterygium Glycosides in the treatment of connective tissue disease-related interstitial lung disease：Meta analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, August 2021. http://dx.doi.org/10.37766/inplasy2021.8.0028.

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He, Mingyu, Tianying Chang, and Shoulin Zhang. Efficacy and safety of Tripterygium wilfordii glycosides in treatment of IgA nephropathy：A systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, January 2022. http://dx.doi.org/10.37766/inplasy2022.1.0037.

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Shi, Hongshuo, Pin Deng, Lei Wang, Wenbin Liu, Yinghao Li, Chengda Dong, Yanfang Wang, Guomin Si, and Tiantian Yang. The Efficacy and Safety of Tripterygium Glycosides for Diabetic Kidney Disease: An Overview of Systematic Reviews and Meta-Analyses. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, March 2022. http://dx.doi.org/10.37766/inplasy2022.3.0065.

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Morrison, Mark, Joshuah Miron, Edward A. Bayer, and Raphael Lamed. Molecular Analysis of Cellulosome Organization in Ruminococcus Albus and Fibrobacter Intestinalis for Optimization of Fiber Digestibility in Ruminants. United States Department of Agriculture, March 2004. http://dx.doi.org/10.32747/2004.7586475.bard.

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Анотація:

Improving plant cell wall (fiber) degradation remains one of the highest priority research goals for all ruminant enterprises dependent on forages, hay, silage, or other fibrous byproducts as energy sources, because it governs the provision of energy-yielding nutrients to the host animal. Although the predominant species of microbes responsible for ruminal fiber degradation are culturable, the enzymology and genetics underpinning the process are poorly defined. In that context, there were two broad objectives for this proposal. The first objective was to identify the key cellulosomal components in Ruminococcus albus and to characterize their structural features as well as regulation of their expression, in response to polysaccharides and (or) P AA/PPA. The second objective was to evaluate the similarities in the structure and architecture of cellulosomal components between R. albus and other ruminal and non-ruminal cellulolytic bacteria. The cooperation among the investigators resulted in the identification of two glycoside hydrolases rate-limiting to cellulose degradation by Ruminococcus albus (Cel48A and CeI9B) and our demonstration that these enzymes possess a novel modular architecture specific to this bacterium (Devillard et al. 2004). We have now shown that the novel X-domains in Cel48A and Cel9B represent a new type of carbohydrate binding module, and the enzymes are not part of a ceiluiosome-like complex (CBM37, Xu et al. 2004). Both Cel48A and Cel9B are conditionally expressed in response to P AA/PPA, explaining why cellulose degradation in this bacterium is affected by the availability of these compounds, but additional studies have shown for the first time that neither PAA nor PPA influence xylan degradation by R. albus (Reveneau et al. 2003). Additionally, the R. albus genome sequencing project, led by the PI. Morrison, has supported our identification of many dockerin containing proteins. However, the identification of gene(s) encoding a scaffoldin has been more elusive, and recombinant proteins encoding candidate cohesin modules are now being used in Israel to verify the existence of dockerin-cohesin interactions and cellulosome production by R. albus. The Israeli partners have also conducted virtually all of the studies specific to the second Objective of the proposal. Comparative blotting studies have been conducted using specific antibodies prepare against purified recombinant cohesins and X-domains, derived from cellulosomal scaffoldins of R. flavefaciens 17, a Clostridium thermocellum mutant-preabsorbed antibody preparation, or against CbpC (fimbrial protein) of R. albus 8. The data also suggest that additional cellulolytic bacteria including Fibrobacter succinogenes S85, F. intestinalis DR7 and Butyrivibrio fibrisolvens Dl may also employ cellulosomal modules similar to those of R. flavefaciens 17. Collectively, our work during the grant period has shown that R. albus and other ruminal bacteria employ several novel mechanisms for their adhesion to plant surfaces, and produce both cellulosomal and non-cellulosomal forms of glycoside hydrolases underpinning plant fiber degradation. These improvements in our mechanistic understanding of bacterial adhesion and enzyme regulation now offers the potential to: i) optimize ruminal and hindgut conditions by dietary additives to maximize fiber degradation (e.g. by the addition of select enzymes or PAA/PPA); ii) identify plant-borne influences on adhesion and fiber-degradation, which might be overcome (or improved) by conventional breeding or transgenic plant technologies and; iii) engineer or select microbes with improved adhesion capabilities, cellulosome assembly and fiber degradation. The potential benefits associated with this research proposal are likely to be realized in the medium term (5-10 years).

Borch, Thomas, Yitzhak Hadar, and Tamara Polubesova. Environmental fate of antiepileptic drugs and their metabolites: Biodegradation, complexation, and photodegradation. United States Department of Agriculture, January 2012. http://dx.doi.org/10.32747/2012.7597927.bard.

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Many pharmaceutical compounds are active at very low doses, and a portion of them regularly enters municipal sewage systems and wastewater-treatment plants following use, where they often do not fully degrade. Two such compounds, CBZ and LTG, have been detected in wastewater effluents, surface waters, drinking water, and irrigation water, where they pose a risk to the environment and the food supply. These compounds are expected to interact with organic matter in the environment, but little is known about the effect of such interactions on their environmental fate and transport. The original objectives of our research, as defined in the approved proposal, were to: Determine the rates, mechanisms and products of photodegradation of LTG, CBZ and selected metabolites in waters exposed to near UV light, and the influence of DOM type and binding processes on photodegradation. Determine the potential and pathways for biodegradation of LTG, CBZ and selected metabolites using a white rot fungus (Pleurotusostreatus) and ADP, and reveal the effect of DOM complexation on these processes. Reveal the major mechanisms of binding of LTG, CBZ and selected metabolites to DOM and soil in the presence of DOM, and evaluate the effect of this binding on their photodegradation and/or biodegradation. We determined that LTG undergoes relatively slow photodegradation when exposed to UV light, and that pH affects each of LTG’s ability to absorb UV light, the efficiency of the resulting reaction, and the identities of LTG’sphotoproducts (t½ = 230 to 500 h during summer at latitude 40 °N). We observed that LTG’sphotodegradation is enhanced in the presence of DOM, and hypothesized that LTG undergoes direct reactions with DOM components through nucleophilic substitution reactions. In combination, these data suggest that LTG’s fate and transport in surface waters are controlled by environmental conditions that vary with time and location, potentially affecting the environment and irrigation waters. We determined that P. ostreatusgrows faster in a rich liquid medium (glucose peptone) than on a natural lignocellulosic substrate (cotton stalks) under SSF conditions, but that the overall CBZ removal rate was similar in both media. Different and more varied transformation products formed in the solid state culture, and we hypothesized that CBZ degradation would proceed further when P. ostreatusand the ᵉⁿᶻʸᵐᵃᵗⁱᶜ ᵖʳᵒᶠⁱˡᵉ ʷᵉʳᵉ ᵗᵘⁿᵉᵈ ᵗᵒ ˡⁱᵍⁿⁱⁿ ᵈᵉᵍʳᵃᵈᵃᵗⁱᵒⁿ. ᵂᵉ ᵒᵇˢᵉʳᵛᵉᵈ ¹⁴C⁻Cᴼ2 ʳᵉˡᵉᵃˢᵉ ʷʰᵉⁿ ¹⁴C⁻ᶜᵃʳᵇᵒⁿʸˡ⁻ labeled CBZ was used as the substrate in the solid state culture (17.4% of the initial radioactivity after 63 days of incubation), but could not conclude that mineralization had occurred. In comparison, we determined that LTG does not degrade in agricultural soils irrigated with treated wastewater, but that P. ostreatusremoves up to 70% of LTG in a glucose peptone medium. We detected various metabolites, including N-oxides and glycosides, but are still working to determine the degradation pathway. In combination, these data suggest that P. ostreatuscould be an innovative and effective tool for CBZ and LTG remediation in the environment and in wastewater used for irrigation. In batch experiments, we determined that the sorption of LTG, CBZ and selected metabolites to agricultural soils was governed mainly by SOM levels. In lysimeter experiments, we also observed LTG and CBZ accumulation in top soil layers enriched with organic matter. However, we detected CBZ and one of its metabolites in rain-fed wheat previously irrigated with treated wastewater, suggesting that their sorption was reversible, and indicating the potential for plant uptake and leaching. Finally, we used macroscale analyses (including adsorption/desorption trials and resin-based separations) with molecular- level characterization by FT-ICR MS to demonstrate the adsorptive fractionation of DOM from composted biosolids by mineral soil. This suggests that changes in soil and organic matter types will influence the extent of LTG and CBZ sorption to agricultural soils, as well as the potential for plant uptake and leaching.