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Добірка наукової літератури з теми "GENE DELIVERY APPLICATIONS"

Автор: Grafiati
Опубліковано: 11 вересня 2023

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Статті в журналах з теми "GENE DELIVERY APPLICATIONS"

1

Huang, Rih-Yang, Zhuo-Hao Liu, Wei-Han Weng, and Chien-Wen Chang. "Magnetic nanocomplexes for gene delivery applications." Journal of Materials Chemistry B 9, no. 21 (2021): 4267–86. http://dx.doi.org/10.1039/d0tb02713h.

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Анотація:
This review paper covers the recent progress of magnetic nanoparticles (MNP)-based gene delivery. Cutting-edge applications of MNP-based gene delivery on cancer therapy, neural repairing, regenerative medicine and gene editing are also introduced.
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2

Chen, Chih-Kuang, Ping-Kuan Huang, Wing-Cheung Law, Chia-Hui Chu, Nai-Tzu Chen, and Leu-Wei Lo. "Biodegradable Polymers for Gene-Delivery Applications." International Journal of Nanomedicine Volume 15 (March 2020): 2131–50. http://dx.doi.org/10.2147/ijn.s222419.

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3

Katz, M. G., A. S. Fargnoli, L. A. Pritchette, and C. R. Bridges. "Gene delivery technologies for cardiac applications." Gene Therapy 19, no. 6 (March 15, 2012): 659–69. http://dx.doi.org/10.1038/gt.2012.11.

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4

Makkonen, Kaisa-Emilia, Kari Airenne, and Seppo Ylä-Herttulala. "Baculovirus-mediated Gene Delivery and RNAi Applications." Viruses 7, no. 4 (April 22, 2015): 2099–125. http://dx.doi.org/10.3390/v7042099.

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5

Suda, Takeshi, and Dexi Liu. "Hydrodynamic Gene Delivery: Its Principles and Applications." Molecular Therapy 15, no. 12 (December 2007): 2063–69. http://dx.doi.org/10.1038/sj.mt.6300314.

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6

Yin, Feng, Bobo Gu, Yining Lin, Nishtha Panwar, Swee Chuan Tjin, Junle Qu, Shu Ping Lau, and Ken-Tye Yong. "Functionalized 2D nanomaterials for gene delivery applications." Coordination Chemistry Reviews 347 (September 2017): 77–97. http://dx.doi.org/10.1016/j.ccr.2017.06.024.

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7

Rabiee, Navid, Shokooh Ahmadvand, Sepideh Ahmadi, Yousef Fatahi, Rassoul Dinarvand, Mojtaba Bagherzadeh, Mohammad Rabiee, Mohammadreza Tahriri, Lobat Tayebi, and Michael R. Hamblin. "Carbosilane dendrimers: Drug and gene delivery applications." Journal of Drug Delivery Science and Technology 59 (October 2020): 101879. http://dx.doi.org/10.1016/j.jddst.2020.101879.

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8

Wich, Peter R., and Jean M. J. Fréchet. "Degradable Dextran Particles for Gene Delivery Applications." Australian Journal of Chemistry 65, no. 1 (2012): 15. http://dx.doi.org/10.1071/ch11370.

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Анотація:
Successful gene therapy depends both on the effective transport and the stable expression of therapeutic genes to produce and regulate disease related proteins. In this context, non-viral gene delivery vehicles are regarded as one of the most promising approaches for the efficient and safe transport of genetic material to and into the target cells. This short review describes the development of novel particulate delivery vehicles based on the biopolymer dextran. This multifunctional platform was designed to safely transport genetic material across cell membranes, followed by an acid triggered release that causes overall high transfection efficiency. The biocompatibility and its unique tunability differentiate this new carrier system from previous particle systems, showing high potential for the treatment of several disease models in RNA interference related applications.
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9

Kafshdooz, Taiebeh, Leila Kafshdooz, Abolfazl Akbarzadeh, Younes Hanifehpour, and Sang Woo Joo. "Applications of nanoparticle systems in gene delivery and gene therapy." Artificial Cells, Nanomedicine, and Biotechnology 44, no. 2 (November 3, 2014): 581–87. http://dx.doi.org/10.3109/21691401.2014.971805.

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10

Contin, Mario, Cybele Garcia, Cecilia Dobrecky, Silvia Lucangioli, and Norma D’Accorso. "Advances in drug delivery, gene delivery and therapeutic agents based on dendritic materials." Future Medicinal Chemistry 11, no. 14 (July 2019): 1791–810. http://dx.doi.org/10.4155/fmc-2018-0452.

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Анотація:
Dendrimers are synthetic polymers that grow in three dimensions into well-defined structures. Their morphological appearance resembles a number of trees connected by a common point. Dendritic nanoparticles have been studied for a large number of pharmaceutical and biomedical applications including gene and drug delivery, clinical diagnosis and MRI. Despite the application of dendrimers, research is still in its childhood in comparison with liposomes and other nanomaterials. They are now playing a key role in several therapeutic strategies, with dendrimer-based products in clinical trials. The aim of this review is to describe the state-of-the-art of biomedical applications of dendrimers – and dendrimer conjugates – such as drug and gene delivery and antiviral activity.
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Більше джерел

Дисертації з теми "GENE DELIVERY APPLICATIONS"

1

Twaites, Beverley Ruth. "Polymer-biopolymer interactions : applications in gene delivery." Thesis, University of Portsmouth, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.402281.

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2

Shaw, Paul Andrew. "Improving gene delivery for gene therapy and DNA vaccination applications." Thesis, University of Cambridge, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.614094.

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3

Cifuentes, Rius Anna. "Tailoring Carbon Nanotubes Properties for Gene Delivery Applications." Doctoral thesis, Universitat Ramon Llull, 2013. http://hdl.handle.net/10803/127706.

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La teràpia gènica s’està convertit en una tècnica innovadora per tal de curar una malaltia mitjançant la inserció de gens dins les cèl•lules i òrgans d’un individu. El repte recau en l’alliberació eficient I segura de l’àcid nucleic terapèutic a les cèl•lules i òrgans objectiu. De tots els vectors sintètics desenvolupats recentment, els nanotubs de carboni són una elecció interessant que ja ha demostrat prometre considerablement com a sistema alliberació, gràcies a la seva proporció amplada-alçada i la seva capacitat de traspassar la membrana cel•lular. El problema que sorgeix és la seva limitada solubilització i l’agregació espontània in vivo. Amb l’objectiu de desenvolupar nous dissenys basats en nanotubs de carboni per a la formació de complexos capaços de transfectar ADN a les cèl•lules, amb un a bon registre de biocompatibilitat i viabilitat cel•lular, s’han desenvolupat diferents estratègies. En primer lloc, s’ha optimitzat la funcionalització covalent dels nanotubs per mitjà de tècniques de plasma. Aquest tipus de modificació permet aconseguir tan superfícies altament reactives capaces d’unir ADN a través d’una molècula enllaçant, com superfícies carregades positivament que permeten l’envolcall de l’àcid nucleic per interacció electrostàtica. En segon lloc, s’ha avaluat la dispersió de nanotubs de diferents mides per mitjà d’un agent estabilitzant incloent un surfactant, un polímer amfifílic i proteïnes. Aquesta naturalesa química de la superfície del nanotub, juntament amb altres propietats físiques com ara l’allargada o el diàmetre, té un efecte directe en la dispersibilitat, citotoxicitat i biodistribució d’aquest sistemes. L’ús de proteïnes per functionalitzar nanopartícules és encoratjador ja que forma la corona de proteïna a la seva superfície. Tals conjugats mostren una elevada capacitat de carregar ADN i permeten la regulació de la seva alliberació mitjançant la manipulació de la composició de la corona.
La terapia génica se está convirtiendo en una técnica innovadora para curar enfermedades mediante la inserción de genes dentro de las células y órganos de un individuo. El reto recae en la liberación eficiente y segura de un acido nucleico terapéutico a los órganos objectivo. De todos los vectores sintéticos desarrollados recientemente, los nanotubos de carbono son una elección interesante que ya ha demostrado prometer considerablemente como sistema de liberación gracias a su proporción anchura-altura y su capacidad de traspasar la membrana celular. El problema que surge es su limitada solubilización i la agregación espontanea in vivo. Con el objetivo de desarrollar nuevos diseños basados en nanotubos de carbono para la formación de complejos capaces te transfectar ADN a las células, con un buen registro de biocompatibilidad y viabilidad celular, se han desarrollado diferentes estrategias. En primer lugar, se ha optimizado la funcionalización covalente de los nanotubos por medio de técnicas de plasma. Este tipo de modificación permite conseguir tanto superficies altamente reactivas capaces de unir ADN a traves de una molécula enlazante, como cargadas positivamente que permiten el envoltorio del acido nucleico por interacción electrostática. En segundo lugar, se han evaluado la dispersión de nanotubos de medidas diferentes por mediado de un agente estabilizante que incluye un surfactante un polímero amfifílico y proteínas. Esta naturaleza química de la superficie del nanotubo, junto con otras propiedades físicas como su longitud o diámetro, tiene un efecto directo en la dispersibilidad, citotoxicidad y biodistribución de estos sitemas. El uso de proteínas para funcionalizar nanopartículas es alentador ya que forma la corona de proteínas en su superficie. Dichos compuestos muestran una elevada capacidad de cargar ADN y permiten la regulación de su liberación mediante la manipulación de la composición de la corona.
Gene therapy has become an increasing innovative technique to treat disease by the insertion of genes into individual’s cells and tissues. The challenge is to efficiently and safely deliver the therapeutic nucleic acid into the target cells and organs. Among the synthetic vectors recently developed, carbon nanotubes are an interesting choice as they have already demonstrated considerable promise as delivery systems due to their high aspect ratio and their capacity to translocate the cell membrane. The problem that arises is their limited solubilization and spontaneous aggregation in vivo. Aiming to engineer new carbon nanotube-based designs for the formation of complexes able to transfect DNA/RNA to cells with a good track of biocompatibility and cell viability, different strategies have been developed. Firstly, the covalent functionalization of carbon nanotubes by plasma techniques has been optimized. This type of modification allows to either achieving highly reactive surfaces able to covalently bind DNA towards a chemical linker or a positively charged nanotube surface enabling the wrapping of the nucleic acid by electrostatic interaction. Secondly, the dispersion of the differently-sized carbon nanotubes by means of a stabilizing agent including a surfactant, an amphiphilic polymer and proteins has been assessed. The chemical nature of the modifying moieties on the carbon nanotube, alongside to other physical properties such as length or diameter, has a direct effect on the dispersibility, cytotoxicity and biodistribution of these systems. The use of proteins in the nanoparticle functionalization is encouraging due to the formation of the protein corona on its surface. Such complex exhibits high DNA load capacities and allows a tunable payload release by manipulating the corona composition
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4

Uthe, Peter Benjamin Ashby Valerie. "The development of polycationic materials for gene delivery applications." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2010. http://dc.lib.unc.edu/u?/etd,2917.

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Анотація:
Thesis (Ph. D.)--University of North Carolina at Chapel Hill, 2010.
Title from electronic title page (viewed Jun. 23, 2010). "... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Chemistry." Discipline: Chemistry; Department/School: Chemistry.
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5

TURCHIANO, GIANDOMENICO. "Defining an innovative and safe non-viral gene delivery system: perspective analysis for gene therapy applications." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2013. http://hdl.handle.net/10281/43579.

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The Sleeping Beauty (SB) transposon-based integration system is a valuable tool for functional genomics in several model organisms and represent a promising vector for gene therapy in humans. The SB transposase was found to bind the inverted repeats of regenerated salmonid transposons in a substrate-specific manner, and to mediate precise cut-and-paste transposition in fish as well as in mouse and human cells. However, a major bottleneck of any transposon-based application is the efficiency of transpositional activity. Therefore, considerable effort has been made to improve the transposition efficiency of SB by modifying its IRs and systematically mutating the transposase gene. The combined effect of these modifications resulted in the generation of the hyperactive SB100X transposase and of the high-capacity “sandwich” transposon (SA). This new system shows an almost 100-fold enhancement of transposition as compared to the first-generation transposon system. I address my work to the molecular characterization of “sandwich” SB-mediated integrants in epithelial cell lines and in primary keratinocytes. The interest in keratinocytes is related to a SB-mediated gene therapy application for the dystrophic form of epidermolysis bullosa (DEB) caused by mutations in the type-VII collagen gene (COL7A1). Delivering the >9 kb COL7A1 cDNA by a retroviral or lentiviral vector is problematic, due to the large size and highly repeated nature of its sequence, which induce genetic rearrangements during reverse transcription and integration. The Sleeping Beauty transposon-based integration system can potentially overcome these issues.
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6

LaManna, Caroline Marie. "Synthesis, characterization, and evaluation of photo-active amphiphiles for gene delivery applications." Thesis, Boston University, 2013. https://hdl.handle.net/2144/12803.

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Анотація:
Thesis (Ph.D.)--Boston University PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you.
Gene therapy has the potential to alter the landscape of medical therapeutic techniques by offering a means of introducing or knocking out genes to treat a number of diseases. Both viral and nonviral vectors are currently being utilized in gene therapy clinical trials. To overcome the obstacles in the cellular uptake and transfection pathways which impede nonviral gene delivery, novel lipids, polymers, and dendrimers are being engineered. Cationic lipid vectors have been widely characterized as gene delivery tools as they electrostatically interact with the anionic nucleic acid backbone to form a supramolecular structure (lipoplex). This complex allows the nucleic acid to be protected from enzymatic degradation during transport and interacts with the cell membrane to facilitate internalization by endocytosis. A limitation of current systems is a lack of mechanism for release of the nucleic acid, which is an integral step toward transcription and translation. The use of a charge-reversal or charge-switching amphiphile has been previously described by which the amphiphile initially has a net positive charge and is rendered negatively charged upon enzymatic removal of a terminal ester group. In order to further improve the transfection efficacy of cationic lipids and to impart an externally controlled release mechanism, we have developed a library of novel photo-active chargereversal lipids which can be triggered by ultraviolet (UV) light. In this work, we describe the synthesis and characterization of photo-active lipids for binding and releasing deoxyribonucleic acid (DNA) and evaluate the cellular uptake kinetics and transfection efficiency in vitro. The binding, release, and cellular uptake behaviors of lipoplexes were found to be dependent on lipid composition and resulting supramolecular structures. The transfection efficiency of the photo-active lipoplexes was further affected by variables associated with cellular incubation and UV exposure. Continued development of controlled release gene delivery vectors, including photoactive lipids, will enhance the understanding and utility of gene therapy by providing spatiotemporal control of the process.
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7

Narayanasamy, Kaarjel Kauslya. "Preparation and evaluation of polymer coated magnetic nanoparticles for applications in gene delivery." Thesis, Keele University, 2018. http://eprints.keele.ac.uk/5002/.

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Анотація:
With the advent of powerful gene editing tools such as CRISPR/Cas9, advances in gene therapy have gained a second wind. Despite this, disease therapy still has not progressed beyond clinical trials due to limitations in current delivery methods. The work presented in this thesis studies the development of a non-viral gene delivery method which is the nanomagnetic transfection method, which is the delivery of genes to cells using magnetic nanoparticles (MNPs) with a cationic surface charge and an external magnetic _eld. The advantage of nanomagnetic transfection over other non-viral chemical methods is the low dosage required to transfect cells coupled with a short transfection time. The presence of an external magnet provides targeting functionality, whereby the MNPs carrying the gene of interest are pulled towards the cells, thus increasing the e_ciency of cell to MNP contact. The research looks at the synthesis of MNPs using thermal decomposition to obtain particles with a narrow size distribution and exhibiting a combination of Brownian and N_eel relaxation. The MNPs were coated with polyethyleneimine (PEI), which binds and condenses DNA to deliver into cells for protein expression. PEI is known to be toxic to cells at high concentrations, hence PEI not bound to MNPs were removed using dialysis. A unique study observing the gradual loading of PEI coating on MNPs using AC susceptometry (ACS) is described. ACS provided information on the MNP coating and aggregation process that was not accessible through dynamic light scattering (DLS) due to the additional presence of non-magnetic polymer particulates in the suspensions. In combination with complementary structural characterization techniques, a simple method was derived to obtain dense, uniform PEI coatings a_ording high-stability suspensions without excessive quantities of unbound PEI to reduce cytotoxic e_ects. This method can be used for improving coating and functionalization therefore advancing MNP-drug/gene delivery studies. The PEI-coated MNPs were subsequently studied for their transfection capabilities in HeLa cells and compared to commercial MNP transfection agents. It was found that nanomagnetic transfection had higher GFP reporter expression compared to Lipofectamine and PEI. The parameters a_ecting transfection activity were determined in order to improve transfection rates of synthesized MNPs. A trade-o_ between transfection e_ciency and cytotoxicity was observed, where the presence of unbound PEI improved transfection but a_ected cell viability. To overcome this, polymers and block-copolymers with a lower charge density should be developed. The proton-sponge e_ect, which is the mechanism of MNP-PEI escape from the endolysosome was studied by measuring the AC susceptibility of MNP-PEI in live cells. However, the low transfection e_ciency of MNP-PEI and low sensitivity of the AC susceptometer made it di_cult to obtain conclusive evidence. A novel study using Raman spectroscopy to obtain _ngerprint spectra of the MNP-PEI complexes and to determine their localization in cells is reported. Individual spectra of MNP and PEI were obtained, as well as the area map of the cell, however the localization of MNPs within the cell was not possible due to the limited sensitivity of the Raman spectrometer. Finally, the e_ect of the MNP-PEI transfection agents on cells were identi_ed. It was observed that MG-63 and HeLa cells expressed increased cell stress with the formation of actin stress _bres and increased cell adhesion. Between the two transfection components, PEI antagonized the cell adhesion e_ect compared to MNPs. In addition, the genes associated with actin _bres and cell adhesion were identi_ed, which were ACTA2, ACTN1, MVCL, VCL, P4HA2, PCDHB12, SVIL, and TGFBI, which showed increased expression to MNP-PEI treatment. Collectively, the study conducted reports the development of an MNP transfection agent, from synthesis to application.
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8

Nelson, Ashley M. "Design of Functional Polyesters for Electronic and Biological Applications." Diss., Virginia Tech, 2015. http://hdl.handle.net/10919/74914.

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Анотація:
Melt polymerization and novel monomers enabled the synthesis of polyesters for electronic and biological applications. Inspiration from nature and a passion for environmental preservation instigated an emphasis on the incorporation of renewable resources into polymeric materials. Critical analysis of current research surrounding bisphenol-A replacements and ioncontaining segmented polyurethanes aided in identifying benchmark polymers, including limitations, challenges, and future needs. Structure-property-morphology relationships were investigated to evaluate the polymers for success in the proposed applications as well as to improve understanding of polyester compositions to further design and develop sophisticated polymers for emerging applications. Aiming to utilize the reported [2 + 2] cycloaddition of the known mesogen 4,4’-dimethyltrans-stilbene dicarboxylate (SDE) to overcome ultraviolet (UV) induced degradation issues in electronic encasings, the synthesis of copolyesters containing SDE ensued. 1,6-Hexanediol (HD) and 1,4-butanediol comonomers in varying weight ratios readily copolymerized with SDE under melt transesterification conditions to afford a systematic series of copolyesters. Differential scanning calorimetry revealed all copolyesters exhibited liquid crystalline transitions and melting temperatures ranged from 196 °C – 317 °C. Additionally, melt rheology displayed shear thinning to facilitate melt processing. Compression molded films exhibited high storage moduli, a glassy plateau until the onset of flow, and tensile testing revealed a Young’s iii modulus of ~900 MPa for poly(SDE-HD). These properties enable a wide range of working temperatures and environments for electronic applications. Adding complexity to linear liquid crystalline copolyesters, copolymerization with oligomeric hydroxyl-functionalized polyethers afforded segmented liquid crystalline copolyesters. 4,4’-Biphenyl dicarboxylate (BDE), commercially available diols containing 4, 5, 6, 8, or 10 methylene units, and introducing poly(tetramethylene oxide) or a Pluronic® triblock oligoethers in varying weight % were used to synthesize multiple series of segmented copolyesters. Comparing melting transitions as a function of methylene spacer length elucidated the expected even-odd effect and melting temperatures ranged from 150 °C to 300 °C. Furthermore, incorporating the flexible soft segment did not prevent formation of a liquid crystalline morphology. Complementary findings between differential scanning calorimetry and small-angle X-ray scattering confirmed a microphase-separated morphology. Thermomechanical analysis revealed tunable plateau moduli and temperature windows based on both soft segment content and methylene spacer length, and tensile testing showed the strain at break doubled from 75 weight % to 50 weight % hard segment content. The same compositions Young’s moduli decreased from 107 ± 12 MPa at 75 weight % hard segment to 19 ± 1 MPa with 50 weight % hard segment, demonstrating the mechanical trade-off and range of properties possible with small compositional changes. These segmented copolyesters could find use in high-performance applications including electronic and aerospace industries. A two-step synthesis transformed caffeine into a novel caffeine-containing methacrylate (CMA). Conventional free radical copolymerization with a comonomer known to provide a low glass transition temperature (Tg), 2-ethylhexyl methacrylate (EHMA), allowed the investigation of the effect of small amounts of pendant caffeine on polymer properties. Thermal and iv thermomechanical testing indicated CMA incorporation dramatically increased the storage modulus, however, a microphase-separated morphology was not attained. Association of the pendant caffeine groups through non-covalent π-π stacking could present opportunities for novel thermoplastics and it is proposed that placing the pendant group further from the backbone, and potentially increasing the concentration, could aid in promoting microphase-separation. Alkenes are reactive sites for placing functional groups, particularly those required for polyester synthesis. Methyl 9-decenoate (9-DAME), a plant-based fatty acid, provided a platform for novel biodegradable, renewable, polyesters. A formic acid hydration reaction generated an isomeric mixture of AB hydroxyester or AB hydroxyacid monomers for melt polymerization. Thermal analysis elucidated the plant-based polyesters exhibited a single transition, a Tg of about -60 °C. Aliphatic polyesters commonly crystallize, thus the isomeric mixture of secondary alcohols seemed to introduce enough irregularity to prevent crystallization. These polyesters offer an amorphous, biodegradable, sustainable replacement for applications currently using semi-crystalline poly(ε-caprolactone), which is not obtained from renewable monomers and also exhibits a -60 °C Tg. Additional applications requiring low-Tg polymers such as pressure sensitive adhesives or thermoplastic elastomers could also benefit from these novel polyesters. 9-DAME also was transformed into an ABB’ monomer after an epoxidation and subsequent hydrolysis. Successful gelation under melt transesterification conditions provided evidence that the multifunctional monomer could perform as a renewable, biodegradable, branching and/or crosslinking agent. Novel copolyesters comprised of a bromomethyl imidazolium diol and adipic acid demonstrated potential as non-viral gene delivery vectors. Melt polycondensation produced water dispersible polyesters which bound deoxyribonucleic acid at low N/P ratios. The v polyplexes showed stability in water over 24 h and no cytotoxic effect on human cervical cancer cells (HeLa). A luciferase transfection assay revealed the copolyesters successfully underwent endocytosis and released the nucleic acid better than controls. The copolyesters with pendant imidazolium functionality also provided tunable Tgs, -41 °C to 40 °C, and the ability to electrospin into fibers upon blending with poly(ethylene oxide). These additional properties furthered potential applications to include pressure sensitive adhesives and biocompatible antibacterial bandages.
Ph. D.
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9

Allen, Michael H. Jr. "Imidazole-Containing Polymerized Ionic Liquids for Emerging Applications: From Gene Delivery to Thermoplastic Elastomers." Diss., Virginia Tech, 2013. http://hdl.handle.net/10919/49593.

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Novel imidazole-containing polyelectrolytes based on poly(1-vinylimidazole) (poly(1VIM)) were functionalized with various hydroxyalkyl-substituents to investigate the influence of charge density and hydrogen bonding on nonviral DNA delivery.  Copolymers with higher charge densities exhibited increased cytotoxicity, whereas increased hydroxyl concentrations remained nontoxic.  DNA binding affinity increased with increased charge densities and increased hydroxyl content.  Dynamic light scattering determined the copolymers which delivered DNA most effectively maintained an intermediate binding affinity between copolymer and DNA.  Copolymers containing higher charge densities or hydroxyl concentrations bound DNA too tightly, preventing its release inside the cell.  Copolymers with lower charge densities failed to protect the DNA from enzymatic degradation.  Tuning hydrogen bonding concentration allowed for a less toxic and more effective alternative to conventional, highly charged polymers for the development of nonviral DNA delivery vehicles.  The synthesis of amine-containing imidazolium copolymers functionalized with low concentrations of folic acid enabled the investigation of additional polymer modifications on nonviral gene delivery.   Functionalization of 1VIM with various hydroxyalkyl and alkyl groups and subsequent conventional free radical polymerization afforded a series of imidazolium-containing polyelectrolytes.  Hydroxyl-containing homopolymers exhibited higher thermal stabilities and lower Tg's compared to the respective alkyl-analog.  X-ray scattering demonstrated the polarity of the hydroxyl group facilitated solvation of the electrostatic interactions disrupting the nanophase-separated morphology observed in the alkylated systems.  Impedance spectroscopy determined hydroxyl-containing imidazolium homopolymers displayed higher ionic conductivities compared to the alkyl-containing analogs which was attributed to increased solvation of electrostatic interactions in the hydroxyl analogs. Beyond functionalizing 1VIM monomers and homopolymers to tailor various properties, the synthesis of novel architectures in a controlled fashion remains difficult due to the radically unstable N-vinyl propagating radical.  The regioisomer 4-vinylimidazole (4VIM) contains two resonance structures affording increased radical stability of the propagating radical.  Nitroxide-mediated polymerization (NMP) and atom transfer radical polymerization (ATRP) failed to control 4VIM homopolymerizations; however, reversible addition-fragmentation chain transfer (RAFT) demonstrated unprecedented control.  Linear pseudo-first order kinetics were observed and successful chain extension with additional 4VIM suggested preservation of the trithiocarbonate functionality. Effectively controlling the polymerization of 4VIM enabled the design of amphoteric block copolymers for emerging applications.  The design of ABA triblock copolymers with 4VIM as a high Tg supporting outer block and di(ethylene glycol) methyl ether methacrylate (DEGMEMA) as a low Tg, inner block, required the development of a new difunctional RAFT chain transfer agent (CTA).  The difunctional CTA successfully mediated the synthesis of the ABA triblock copolymer, poly(4VIM-b-DEGMEMA-b-4VIM), which exhibited microphase separated morphologies.  The amphoteric nature of the imidazole ring required substantially lower concentrations of outer block incorporation compared to traditional triblock copolymers to achieve similar mechanical properties and microphase separated morphologies.
Ph. D.
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10

Perouzel, Eric. "Synthesis formulation and applications of new stabilisation agents for liposome based gene delivery system." Thesis, Imperial College London, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271482.

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Книги з теми "GENE DELIVERY APPLICATIONS"

1

M, Amiji Mansoor, ed. Polymeric gene delivery: Principles and applications. Boca Raton, Fla: CRC Press, 2005.

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Twaites, Beverley Ruth. Polymer-biopolymer interactions: Applications in gene delivery. Portsmouth: University of Portsmouth, School of Pharmacy and Biomedical Sciences, 2004.

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D, Lasic D., and Papahadjopoulos Demetrios, eds. Medical applications of liposomes. Amsterdam: Elsevier, 1998.

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4

Bremner, K. Helen. Application of nuclear localization sequences to non-viral gene delivery systems. Birmingham: University of Birmingham, 2002.

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5

Carlisle, Robert. The application of adenovirus transduction mechanisms to enhance the activity of synthetic gene delivery systems. Birmingham: University of Birmingham, 2002.

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6

Zimmer, Vanessa. Gene Delivery: Methods and Applications. Nova Science Publishers, Incorporated, 2019.

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7

Gene Delivery: Methods and Applications. Nova Science Publishers, Incorporated, 2019.

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8

Amiji, Mansoor M. Polymeric Gene Delivery: Principles and Applications. CRC, 2004.

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9

Amiji, Mansoor M. Polymeric Gene Delivery: Principles and Applications. Taylor & Francis Group, 2004.

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10

Amiji, Mansoor M. Polymeric Gene Delivery: Principles and Applications. Taylor & Francis Group, 2004.

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Частини книг з теми "GENE DELIVERY APPLICATIONS"

1

Amponsah, Seth Kwabena, Ismaila Adams, and Kwasi Agyei Bugyei. "Clinical Applications of siRNA." In Gene Delivery Systems, 65–76. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003186069-4.

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2

Trimal, Kavita, and Kalpana Joshi. "COVID-19 Vaccine Development and Applications." In Gene Delivery Systems, 197–221. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003186069-12.

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3

Barua, Sonia, and Yashwant Pathak. "siRNA Delivery for Therapeutic Applications Using Nanoparticles." In Gene Delivery Systems, 103–13. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003186083-8.

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4

Faldu, Khushboo, Sakshi Gurbani, and Jigna Shah. "Clinical Applications of Gene Therapy for Immuno-Deficiencies." In Gene Delivery Systems, 195–206. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003186083-14.

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5

Pandey, Prachi, Jayvadan Patel, and Samarth Kumar. "CRISPER Gene Therapy Recent Trends and Clinical Applications." In Gene Delivery Systems, 179–94. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003186083-13.

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6

Patel, Kshama, Preetam Dasika, and Yashwant V. Pathak. "The Current State of Non-Viral Vector–Based mRNA Medicine Using Various Nanotechnology Applications." In Gene Delivery Systems, 89–103. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003186069-6.

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7

Santhakumaran, Latha M., Alex Chen, C. K. S. Pillai, Thresia Thomas, Huixin He, and T. J. Thomas. "Nanotechnology in Nonviral Gene Delivery." In Nanofabrication Towards Biomedical Applications, 251–87. Weinheim, FRG: Wiley-VCH Verlag GmbH & Co. KGaA, 2005. http://dx.doi.org/10.1002/3527603476.ch10.

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8

Halley, Patrick D., Christopher R. Lucas, Nikša Roki, Nicholas J. Vantangoli, Kurtis P. Chenoweth, and Carlos E. Castro. "DNA Origami Nanodevices for Therapeutic Delivery Applications." In Biotechnologies for Gene Therapy, 161–94. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-93333-3_8.

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Šebestík, Jaroslav, Milan Reiniš, and Jan Ježek. "Dendrimers in Gene Delivery." In Biomedical Applications of Peptide-, Glyco- and Glycopeptide Dendrimers, and Analogous Dendrimeric Structures, 141–47. Vienna: Springer Vienna, 2012. http://dx.doi.org/10.1007/978-3-7091-1206-9_14.

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Dev Jayant, Rahul, Abhijeet Joshi, Ajeet Kaushik, Sneham Tiwari, Rashmi Chaudhari, Rohit Srivastava, and Madhavan Nair. "Chapter 8. Nanogels for Gene Delivery." In Nanogels for Biomedical Applications, 128–42. Cambridge: Royal Society of Chemistry, 2017. http://dx.doi.org/10.1039/9781788010481-00128.

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Тези доповідей конференцій з теми "GENE DELIVERY APPLICATIONS"

1

Dandia, Hiren, Snehal Valvi, Rahul Thorat, Arvind Ingle, Abhijit De, Shubhada Chiplunkar, and Prakriti Tayalia. "Scaffold Based Gene Delivery for Immunotherapeutic Applications." In National Research Scholars' Meet 2021 - Abstracts. Thieme Medical and Scientific Publishers Pvt. Ltd., 2022. http://dx.doi.org/10.1055/s-0042-1755513.

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2

Flick, Eva, Wenzhong Li, Jonas Norpoth, Christian Jooss, Gustav Steinhoff, and Hans H. Gatzen. "Characterization of a Magnetic Microactuator for Manipulating Nanoparticles in Gene Delivery Applications." In ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASMEDC, 2010. http://dx.doi.org/10.1115/nemb2010-13025.

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Magnetically driven gene delivery provides a valuable approach for a single cell transfection. Therapeutic genes can be manipulated by conjugating them to magnetic nanoparticles. The magnetic force required for the manipulation of such complexes can be provided by an electromagnetic micro-actuator designed as micro pole row. The analytical calculations, simulations, and the thin-film fabrication of this micro pole row were carried out previously [1]. This paper presents characterization results of the first microactuator fabrication batch.
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3

Olton, Dana, Dong Hyun Lee, Charles Sfeir, and Prashant N. Kumta. "Novel Nanostructured Calcium Phosphate Based Delivery Systems for Non-Viral Gene Delivery." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176286.

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Calcium phosphate (CaP) based approaches remain an attractive option for delivering plasmid DNA (pDNA) into cultured cells [1, 2]. However, two major limitations associated with this vector exist. First, it yields lower transfection efficiencies with respect to its’ viral counterparts. Second, CaP mediated gene delivery leads to transient transgene expression. Thus, we hypothesized that these concerns could respectively be addressed by: (1) synthesizing particles with precise control of the materials’ parameters including (i.e. Ca/P ratio, particle size, crystal structure, and microstructure) and (2) incorporating the particles into a 3-D biodegradable fibrin scaffold. The goal of this study was therefore to synthesize and optimize the efficacy of both nano-structured CaP (NanoCaPs) particles and a composite scaffold comprised of fibrin, CaP and pDNA for non-viral gene delivery applications.
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4

Weibing Lu, Hilal Gul, Peng Xu, Woon T. Ang, James Xing, Jian Zhang, and Jie Chen. "A novel gene delivery system using magnetic nanodarts." In 2009 IEEE/NIH Life Science Systems and Applications Workshop (LiSSA) Formerly known as LSSA and. IEEE, 2009. http://dx.doi.org/10.1109/lissa.2009.4906738.

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5

Jones, Frank R., Elizabeth S. Gabitzsch, and Joseph P. Balint. "The Ad5 [E1-, E2b-]-based vector: a new and versatile gene delivery platform." In SPIE Sensing Technology + Applications, edited by Šárka O. Southern. SPIE, 2015. http://dx.doi.org/10.1117/12.2183244.

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6

Wong, Peter, Michael A. Choi, Hilal Gul-Uludag, Woon T. Ang, Peng Xu, James Xing, and Jie Chen. "Ultrasound-mediated gene delivery into hard-to-transfect KG-1 cells." In 2011 IEEE/NIH 5th Life Science Systems and Applications Workshop (LiSSA). IEEE, 2011. http://dx.doi.org/10.1109/lissa.2011.5754179.

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7

Serša, Gregor. "CLINICAL APPLICATIONS OF ELECTROCHEMOTHERAPY." In Symposium with International Participation HEART AND … Akademija nauka i umjetnosti Bosne i Hercegovine, 2019. http://dx.doi.org/10.5644/pi2019.181.01.

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Electroporation has several biomedical and industrial applications. The biomedical applications are in the field of drug or gene delivery. Electrochemotherapy utilizes electroporation for the increased delivery of cytotoxic drugs like bleomycin or cisplatin into tumors. The use of electrochemotherapy has spread throughout Europe for the treatment of cutaneous tumors or metastases. It is in the NICE guidelines and is becoming standard ablative technique in treatment of cancer. The technological advancements have also enabled the use of electrochemotherapy for the treatment of deep seated tumors, such as soft tissue or liver tumors. Clinical studies demonstrate good effectiveness on fibrosarcomas, colorectal liver metastases and hepatocellular carcinoma. However, electrochemotherapy is a local treatment that also induces moderate local immune response. This so called “in situ vaccination” induced by electrochemotherapy can be exploited in combined treatment with immune checkpoint inhibitors or electrogene therapy with immunostimulating effect. Therefore, gene electrotransfer of plasmid coding for interleukin 12 (IL-12), in combination with electrochemotherapy could result in transformation of electrochemotherapy from local into systemic treatment. This is also of our current interest, and we are undertaking steps to bring this idea from preclinical into clinical testing.
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Brown, Paige K., Ammar T. Qureshi, Daniel J. Hayes, and W. Todd Monroe. "Targeted Gene Silencing With Light and a Silver Nanoparticle Antisense Delivery System." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53647.

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Targeted delivery and controlled release of oligonucleotide therapeutics in vivo are essential aspects of an ideal delivery vehicle. Here we demonstrate the synthesis and in vitro/intracellular characterization of silver nanoparticle (SNP) photolabile nucleic acid conjugates, with the aim of developing a nanoparticulate platform for inducible gene silencing. Due to unique size related properties, nanostructures are being increasingly utilized for intracellular diagnostics and delivery applications. While most nanoscale delivery platforms are polymeric in composition, studies of metallic nanoparticles have highlighted their suitability for delivery of therapeutic agents such as antisense oligonucleotides [1]. The potential benefits of noble metal nanoparticles in delivery applications include tunable size and shape, ease of bulk synthesis and functionalization via ‘wet chemistry’ techniques, and enhanced stability of tethered DNA [2]. Silver is one of the best surface-enhancing substrates available for nanostructure synthesis [3]. SNP composites afford external control over surface-tethered drug release via external triggers.
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9

Vlaskou, Dialechti, Pallab Pradhan, Christian Bergemann, Alexander L. Klibanov, Karin Hensel, Georg Schmitz, Christian Plank, et al. "Magnetic Microbubbles: Magnetically Targeted and Ultrasound-Triggered Vectors for Gene Delivery in Vitro." In 8TH INTERNATIONAL CONFERENCE ON THE SCIENTIFIC AND CLINICAL APPLICATIONS OF MAGNETIC CARRIERS. AIP, 2010. http://dx.doi.org/10.1063/1.3530059.

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Delyagina, Evgenya, Wenzhong Li, Anna Schade, Anna-L. Kuhlo, Nan Ma, Gustav Steinhoff, Urs Häfeli, Wolfgang Schütt, and Maciej Zborowski. "Low Molecular Weight Polyethyleneimine Conjugated to Magnetic Nanoparticles as a Vector for Gene Delivery." In 8TH INTERNATIONAL CONFERENCE ON THE SCIENTIFIC AND CLINICAL APPLICATIONS OF MAGNETIC CARRIERS. AIP, 2010. http://dx.doi.org/10.1063/1.3530058.

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Звіти організацій з теми "GENE DELIVERY APPLICATIONS"

1

Radu, Daniela Rodica. Mesoporous Silica Nanomaterials for Applications in Catalysis, Sensing, Drug Delivery and Gene Transfection. Office of Scientific and Technical Information (OSTI), January 2004. http://dx.doi.org/10.2172/837277.

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2

Hackett, Kevin, Shlomo Rottem, David L. Williamson, and Meir Klein. Spiroplasmas as Biological Control Agents of Insect Pests. United States Department of Agriculture, July 1995. http://dx.doi.org/10.32747/1995.7613017.bard.

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Toward development of spiroplasmas as novel toxin-delivery systems for biocontrol of beetle pests in the United States (Leptinotarsa decemlineata) and Israel (Maladera matrida), media for cultivating beetle-associated spiroplasmas were improved and surveys of these spiroplasmas were conducted to provide transformable strains. Extensive surveys of spiroplasmas yielded promising extrachromosomal elements for vector constructs. One, plasmid pCT-1, was cloned, characterized, and used as a source of spiroplasma origin of replication in our shuttle vectors. The fibrillin gene was isolated and sequenced and its strong promoter was also used in the constructs. Means for transforming these vectors into spiroplasmas were developed and optimized, with electroporation found to be suitable for most applications. Development and optimization of means for using large unilamellar vesicles (LUVs) in spiroplasma transformation represents a breakthrough that should facilitate insertion of large clusters of virulence genes. With completion of the vector, we should thus be poised to genetically engineer spiroplasmas with genes that will express toxins lethal to our target beetles, thus providing an effective and inexpensive alternative to conventional means of beetle control.
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