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Добірка наукової літератури з теми "GCAPs"

Автор: Grafiati

Опубліковано: 11 березня 2023

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Статті в журналах з теми "GCAPs"

Ames, James B. "Structural Insights into Retinal Guanylate Cyclase Activator Proteins (GCAPs)." International Journal of Molecular Sciences 22, no. 16 (August 13, 2021): 8731. http://dx.doi.org/10.3390/ijms22168731.

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Retinal guanylate cyclases (RetGCs) promote the Ca2+-dependent synthesis of cGMP that coordinates the recovery phase of visual phototransduction in retinal rods and cones. The Ca2+-sensitive activation of RetGCs is controlled by a family of photoreceptor Ca2+ binding proteins known as guanylate cyclase activator proteins (GCAPs). The Mg2+-bound/Ca2+-free GCAPs bind to RetGCs and activate cGMP synthesis (cyclase activity) at low cytosolic Ca2+ levels in light-activated photoreceptors. By contrast, Ca2+-bound GCAPs bind to RetGCs and inactivate cyclase activity at high cytosolic Ca2+ levels found in dark-adapted photoreceptors. Mutations in both RetGCs and GCAPs that disrupt the Ca2+-dependent cyclase activity are genetically linked to various retinal diseases known as cone-rod dystrophies. In this review, I will provide an overview of the known atomic-level structures of various GCAP proteins to understand how protein dimerization and Ca2+-dependent conformational changes in GCAPs control the cyclase activity of RetGCs. This review will also summarize recent structural studies on a GCAP homolog from zebrafish (GCAP5) that binds to Fe2+ and may serve as a Fe2+ sensor in photoreceptors. The GCAP structures reveal an exposed hydrophobic surface that controls both GCAP1 dimerization and RetGC binding. This exposed site could be targeted by therapeutics designed to inhibit the GCAP1 disease mutants, which may serve to mitigate the onset of retinal cone-rod dystrophies.

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Imanishi, Yoshikazu, Lili Yang, Izabela Sokal, S?awomir Filipek, Krzysztof Palczewski, and Wolfgang Baehr. "Diversity of Guanylate Cyclase-Activating Proteins (GCAPs) in Teleost Fish: Characterization of Three Novel GCAPs (GCAP4, GCAP5, GCAP7) from Zebrafish (Danio rerio) and Prediction of Eight GCAPs (GCAP1-8) in Pufferfish (Fugu rubripes)." Journal of Molecular Evolution 59, no. 2 (August 2004): 204–17. http://dx.doi.org/10.1007/s00239-004-2614-y.

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Gorczyca, Wojciech A., Marcin Kobiałka, Marianna Kuropatwa, and Ewa Kurowska. "Ca2+ differently affects hydrophobic properties of guanylyl cyclase-activating proteins (GCAPs) and recoverin." Acta Biochimica Polonica 50, no. 2 (June 30, 2003): 367–76. http://dx.doi.org/10.18388/abp.2003_3691.

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Guanylyl cyclase-activating proteins (GCAPs) and recoverin are retina-specific Ca(2+)-binding proteins involved in phototransduction. We provide here evidence that in spite of structural similarities GCAPs and recoverin differently change their overall hydrophobic properties in response to Ca(2+). Using native bovine GCAP1, GCAP2 and recoverin we show that: i) the Ca(2+)-dependent binding of recoverin to Phenyl-Sepharose is distinct from such interactions of GCAPs; ii) fluorescence intensity of 1-anilinonaphthalene-8-sulfonate (ANS) is markedly higher at high [Ca(2+)](free) (10 microM) than at low [Ca(2+)](free) (10 nM) in the presence of recoverin, while an opposing effect is observed in the presence of GCAPs; iii) fluorescence resonance energy transfer from tryptophane residues to ANS is more efficient at high [Ca(2+)](free) in recoverin and at low [Ca(2+)](free) in GCAP2. Such different changes of hydrophobicity evoked by Ca(2+) appear to be the precondition for possible mechanisms by which GCAPs and recoverin control the activities of their target enzymes.

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Dejda, Agnieszka, Izabela Matczak, and Wojciech A. Gorczyca. "p19 detected in the rat retina and pineal gland is a guanylyl cyclase-activating protein (GCAP)." Acta Biochimica Polonica 49, no. 4 (December 31, 2002): 899–905. http://dx.doi.org/10.18388/abp.2002_3749.

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The Ca(2+)-dependent activation of retina-specific guanylyl cyclase (retGC) is mediated by guanylyl cyclase-activating proteins (GCAPs). Here we report for the first time detection of a 19 kDa protein (p19) with GCAP properties in extracts of rat retina and pineal gland. Both extracts stimulate synthesis of cGMP in rod outer segment (ROS) membranes at low (30 nM) but not at high (1 microM) concentrations of Ca(2+). At low Ca(2+), immunoaffinity purified p19 activates guanylyl cyclase(s) in bovine ROS and rat retinal membranes. Moreover, p19 is recognized by antibodies against bovine GCAP1 and, similarly to other GCAPs, exhibits a Ca(2+)-dependent electrophoretic mobility shift.

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Vinberg, Frans, Teemu T. Turunen, Hanna Heikkinen, Marja Pitkänen, and Ari Koskelainen. "A novel Ca2+-feedback mechanism extends the operating range of mammalian rods to brighter light." Journal of General Physiology 146, no. 4 (September 28, 2015): 307–21. http://dx.doi.org/10.1085/jgp.201511412.

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Sensory cells adjust their sensitivity to incoming signals, such as odor or light, in response to changes in background stimulation, thereby extending the range over which they operate. For instance, rod photoreceptors are extremely sensitive in darkness, so that they are able to detect individual photons, but remain responsive to visual stimuli under conditions of bright ambient light, which would be expected to saturate their response given the high gain of the rod transduction cascade in darkness. These photoreceptors regulate their sensitivity to light rapidly and reversibly in response to changes in ambient illumination, thereby avoiding saturation. Calcium ions (Ca2+) play a major role in mediating the rapid, subsecond adaptation to light, and the Ca2+-binding proteins GCAP1 and GCAP2 (or guanylyl cyclase–activating proteins [GCAPs]) have been identified as important mediators of the photoreceptor response to changes in intracellular Ca2+. However, mouse rods lacking both GCAP1 and GCAP2 (GCAP−/−) still show substantial light adaptation. Here, we determined the Ca2+ dependency of this residual light adaptation and, by combining pharmacological, genetic, and electrophysiological tools, showed that an unknown Ca2+-dependent mechanism contributes to light adaptation in GCAP−/− mouse rods. We found that mimicking the light-induced decrease in intracellular [Ca2+] accelerated recovery of the response to visual stimuli and caused a fourfold decrease of sensitivity in GCAP−/− rods. About half of this Ca2+-dependent regulation of sensitivity could be attributed to the recoverin-mediated pathway, whereas half of it was caused by the unknown mechanism. Furthermore, our data demonstrate that the feedback mechanisms regulating the sensitivity of mammalian rods on the second and subsecond time scales are all Ca2+ dependent and that, unlike salamander rods, Ca2+-independent background-induced acceleration of flash response kinetics is rather weak in mouse rods.

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Peshenko, Igor V., Elena V. Olshevskaya, and Alexander M. Dizhoor. "GUCY2D mutations in retinal guanylyl cyclase 1 provide biochemical reasons for dominant cone–rod dystrophy but not for stationary night blindness." Journal of Biological Chemistry 295, no. 52 (October 27, 2020): 18301–15. http://dx.doi.org/10.1074/jbc.ra120.015553.

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Mutations in the GUCY2D gene coding for the dimeric human retinal membrane guanylyl cyclase (RetGC) isozyme RetGC1 cause various forms of blindness, ranging from rod dysfunction to rod and cone degeneration. We tested how the mutations causing recessive congenital stationary night blindness (CSNB), recessive Leber's congenital amaurosis (LCA1), and dominant cone–rod dystrophy-6 (CORD6) affected RetGC1 activity and regulation by RetGC-activating proteins (GCAPs) and retinal degeneration-3 protein (RD3). CSNB mutations R666W, R761W, and L911F, as well as LCA1 mutations R768W and G982VfsX39, disabled RetGC1 activation by human GCAP1, -2, and -3. The R666W and R761W substitutions compromised binding of GCAP1 with RetGC1 in HEK293 cells. In contrast, G982VfsX39 and L911F RetGC1 retained the ability to bind GCAP1 in cyto but failed to effectively bind RD3. R768W RetGC1 did not bind either GCAP1 or RD3. The co-expression of GUCY2D allelic combinations linked to CSNB did not restore RetGC1 activity in vitro. The CORD6 mutation R838S in the RetGC1 dimerization domain strongly dominated the Ca2+ sensitivity of cyclase regulation by GCAP1 in RetGC1 heterodimer produced by co-expression of WT and the R838S subunits. It required higher Ca2+ concentrations to decelerate GCAP-activated RetGC1 heterodimer—6-fold higher than WT and 2-fold higher than the Ser838-harboring homodimer. The heterodimer was also more resistant than homodimers to inhibition by RD3. The observed biochemical changes can explain the dominant CORD6 blindness and recessive LCA1 blindness, both of which affect rods and cones, but they cannot explain the selective loss of rod function in recessive CSNB.

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Neuman, L. M., M. Van Nieuwland, M. Vermeer, D. Boumans, E. Colin, and C. Alves. "AB0375 EXTERNAL VALIDATION OF THE GIANT CELL ARTERITIS PROBABILITY SCORE IN THE NETHERLANDS." Annals of the Rheumatic Diseases 80, Suppl 1 (May 19, 2021): 1214–15. http://dx.doi.org/10.1136/annrheumdis-2021-eular.2466.

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Background:Severe complications of giant cell arteritis (GCA), such as blindness and ischemic stroke, can be prevented by timely treatment. This requires early and accurate diagnosis. In 2019, Laskou et al. developed a clinical GCA probability score (GCAPS) that allows physicians to assess the probability of GCA at initial assessment. The GCAPS is suitable for easy implementation in daily practice. It has a high sensitivity (95.7%) and specificity (86.7%) at a cut-off value of 9.5 as proposed by Laskou et al.Objectives:The aim of this study was to externally validate the GCAPS in a general hospital.Methods:A retrospective cohort of patients with suspected GCA between January 1st 2017 and October 1st 2019 at Ziekenhuisgroep Twente (the Netherlands) was used. As the parameter extra-cranial artery abnormality was not available in our cohort, a modified version of the GCAPS was used (m-GCAPS). The m-GCAPS was compared to clinical diagnosis of a rheumatologist after six months in accordance with Laskou et al. The validity of the m-GCAPS was assessed by plotting a receiver operating characteristic (ROC) curve and assessing sensitivity and specificity for multiple cut-off values. For practical purposes, risk stratifications as proposed by Sebastian et al. (2020) were applied to our data (low-risk <9; intermediate-risk 9-12; high-risk >12).Results:In our cohort, 40 GCA patients and 95 controls (suspected of GCA) had complete records and were therefore used for analysis. In the patient group, 29 (72.5%) were female and mean (SD) age was 73.3 (1.6). The area under the ROC curve (AUC) was 0.83 (95% CI 0.75 – 0.91). At our optimal cut-off value of 10.5, sensitivity was 80.0% and specificity was 75.8%. In total, 5 patients and 45 controls were stratified as low, 7 patients and 35 controls as intermediate and 28 patients and 15 controls as high-risk.Conclusion:The m-GCAPS showed good discrimination in our cohort. Risk stratification of patients looks promising, although cut-off values by Sebastian et al. might need optimization based on population and modification to the GCAPS. Colour Doppler ultrasound is recommended by EULAR as the first-choice diagnostic test to confirm GCA in case of high pre-test probability and exclude GCA for low pre-test probability. The (m-)GCAPS may aid in quantification of this pre-test probability.References:[1]Laskou F, Coath F, Mackie SL et al. A probability score to aid the diagnosis of suspected giant cell arteritis. Clin Exp Rheumatol. 2019;37 Suppl 1(2):104–8.[2]Sebastian A, Tomelleri A, Kayani A et al. Probability-based algorithm using ultrasound and additional tests for suspected GCA in a fast-track clinic. RMD Open. 2020 Sep 1;6(3):e001297.[3]Dejaco C, Ramiro S, Duftner C et al. EULAR recommendations for the use of imaging in large vessel vasculitis in clinical practice. Ann Rheum Dis. 2018 May 1;77(5):636 LP – 643.Table 1.Cut-off values of the m-GCAPS stratified into risk categories as proposed by Sebastian et al. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) and the number of patients scoring above or below the cut-off values are described (optimal cut-off value in blue). The total number of patients with a score within low- (red), intermediate- (orange) and high- (green) risk categories are depicted.GCA+ (N)N=40GCA- (N)N=95Scorem-GCAPSGCA+ (N)GCA- (N)Sens. (%)Spec. (%)PPV (%)NPV (%)Low-risk <95 (12.5%)45 (47.4%)5.5<5.501410014.733.1100>5.540818.5<8.554587.547.441.290>8.53550Intermediate-risk9-127 (17.5%)35 (36.8%)9.5<9.58568058.945.187.5>9.5323910.5<10.58728075.858.290>10.5322311.5<11.510747577.958.888.1>11.53021High-risk>928 (70.0%)15 (15.8%)12.5<12.512807084.265.187>12.5281514.5<14.518895593.778.683.2>14.522619.5<19.537957.510010072>19.530Figure 1.ROC curve depicting sensitivity and (1-) specificity. The AUC was 0.83 (95.0% CI 0.75-0.91) with an optimal cut-off value of 10.5 (sensitivity 80.0%, specificity 75.8%).Disclosure of Interests:None declared

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Ingram, Norianne T., Alapakkam P. Sampath, and Gordon L. Fain. "Voltage-clamp recordings of light responses from wild-type and mutant mouse cone photoreceptors." Journal of General Physiology 151, no. 11 (September 27, 2019): 1287–99. http://dx.doi.org/10.1085/jgp.201912419.

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We describe the first extensive study of voltage-clamp current responses of cone photoreceptors in unlabeled, dark-adapted mouse retina using only the position and appearance of cone somata as a guide. Identification was confirmed from morphology after dye filling. Photocurrents recorded from wild-type mouse cones were biphasic with a fast cone component and a slower rod component. The rod component could be eliminated with dim background light and was not present in mouse lines lacking the rod transducin-α subunit (Gnat1−/−) or connexin 36 (Cx36−/−). Cones from Gnat1−/− or Cx36−/− mice had resting membrane potentials between −45 and −55 mV, peak photocurrents of 20–25 picoamps (pA) at a membrane potential Vm = −50 mV, sensitivities 60–70 times smaller than rods, and a total membrane capacitance two to four times greater than rods. The rate of activation (amplification constant) was largely independent of the brightness of the flash and was 1–2 s−2, less than half that of rods. The role of Ca2+-dependent transduction modulation was investigated by recording from cones in mice lacking rod transducin (Gnat1), recoverin, and/or the guanylyl-cyclase–activating proteins (GCAPs). In confirmation of previous results, responses of Gnat1−/−;Gcaps−/− cones and triple-mutant Gnat1−/−;Gcaps−/−;Rv−/− cones recovered more slowly both to light flashes and steps and were more sensitive than cones expressing the GCAPs. Cones from all four mouse lines showed significant recovery and escaped saturation even in bright background light. This recovery occurred too rapidly to be caused by pigment bleaching or metaII decay and appears to reflect some modulation of response inactivation in addition to those produced by recoverin and the GCAPs. Our experiments now make possible a more detailed understanding of the cellular physiology of mammalian cone photoreceptors and the role of conductances in the inner and outer segment in producing cone light responses.

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Baehr, Wolfgang, and Krzysztof Palczewski. "Focus on Molecules: Guanylate cyclase-activating proteins (GCAPs)." Experimental Eye Research 89, no. 1 (June 2009): 2–3. http://dx.doi.org/10.1016/j.exer.2008.12.016.

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Peshenko, Igor V., and Alexander M. Dizhoor. "Guanylyl Cyclase-activating Proteins (GCAPs) Are Ca2+/Mg2+Sensors." Journal of Biological Chemistry 279, no. 17 (March 1, 2004): 16903–6. http://dx.doi.org/10.1074/jbc.c400065200.

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Дисертації з теми "GCAPs"

Prévèreau, Audrey-Anne. "Liaison membranaire et étude spectroscopique de la GCAP1." Master's thesis, Université Laval, 2014. http://hdl.handle.net/20.500.11794/25622.

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Les protéines activatrices de la guanylate cyclase (GCAPs) font partie de la famille des neuroprotéines sensibles au Ca²⁺ (NCS) et celle des protéines à EF-Hand. Il a été proposé que le mécanisme de Ca²⁺-myristoyl switch avait lieu chez toutes les protéines de la famille des NCS. Les travaux présentés dans ce mémoire permettent de déterminer si ce mécanisme est observé chez la GCAP1. En effet, des travaux de liaison membranaire à des monocouches de Langmuir effectués avec la GCAP1 ont permis d’observer ce mécanisme. De plus, l’utilisation d’un analogue du myristoyle, le 13-oxa-myristoyle, a aussi permis d’observer un Ca²⁺-myristoyl switch chez la GCAP1. Effectivement, des mesures en résonance magnétique nucléaire (RMN) ont démontré que la présence de cet analogue favorise l’extrusion du myristoyle. Finalement, différentes analyses par RMN ont été effectuées afin de déterminer si cette méthode pourrait permettre de déterminer la structure de la forme active de la GCAP1.

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Janski, Natacha. "Étude du mécanisme de nucléation des microtubules chez les plantes supéreures : Caractérisation des protéines GCPs et GIPs d’Arabidopsis thaliana." Université Louis Pasteur (Strasbourg) (1971-2008), 2007. https://publication-theses.unistra.fr/restreint/theses_doctorat/2007/JANSKI_Natacha_2007.pdf.

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Les microtubules (MTs) constituent l’un des réseaux du cytosquelette des cellules eucaryotes. Chez les plantes supérieures, les MTs montrent une importante dynamique de réorganisation au cours du cycle cellulaire. Cette dynamique est notamment liéé à la formation des MTs de novo par des protéines dites de « nucléation » présentes aux centres organisateurs de microtubules (MTOCs). Les mécanismes moléculaires de nucléation restent mal connus mais semblent conservés chez les eucaryotes. La -tubuline et les protéines GCPs (Gamma-tubulin Complex Proteins) ont été caractérisées chez les levures et les animaux comme étant impliquées dans la nucléation. Il est actuellement admis que ces complexes multiprotéiques eucaryotes formés dans le cytoplasme sont recrutés aux MTOCs où leur activation conduit à la nucléation des MTs. Les gènes orthologues d’Arabidopsis thaliana codant pour les AtGCP1-6 ont été identifiés. Les expériences menées lors de cette thèse montrent que les AtGCP1-3 interagissent entre elles in vivo. Les études de localisation ont permis d’identifier de nouveaux signaux d’adressage aux sites MTOCs des plantes. D’autre part, une nouvelle famille de protéines eucaryotes a été identifiée : les GIPs (GCP3 Interacting Proteins). Les AtGIPs, outre leurs interactions avec la protéine de nucléation AtGCP3 et la protéine TSA (Tonsoku Associated protein), sont retrouvées associées aux différents réseaux de MTs, tant chez l’animal que chez les plantes. Les travaux effectués lors de cette thèse ouvrent de nouvelles perspectives de recherche sur la régulation de l’activité des complexes de nucléation et ses implications lors du déroulement de la division cellulaire
Microtubules (MTs) are one of the cytoskeletal network of eukaryotic cells. In higher plants, MTs show an important dynamic leading to their reorganization throughout the cell cycle. This dynamic is in great part linked to de novo MTs formation, thanks to « nucleation » proteins found in MicroTubule Organizing Centers (MTOCs). The molecular mechanisms of nucleation are not well understood, but seem to be conserved in eukaryotes. Gamma-tubulin and GCPs (Gamma-tubulin Complex Proteins) have been shown in yeasts and animals to be involved in nucleation. It is admited that these proteins assembled in the cytoplasm into complexes recruted to MTOCs. Then, there are activated and able to nucleate a new MT. Orthologues encoding AtGCP1-6 have been identified in Arabidopsis thaliana. Experimentations done during this PhD show that most conserved AtGCPs interact together in vivo. Thanks to localization studies, new plant MTOC addressing sequences in GCP proteins have been characterized. Furthermore, a new eucaryotic protein familly has been identified : GIPs (GCP3 Interacting Proteins). AtGIPs interact with AtGCP3 and TSA (Tonsoku Associated protein), and moreover are associated with MTs networks, both in plants and animals. This work contributes to enlarge perspectives concerning nucleation complex research and regulation during cell division

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Lima, Bruno Miguel Ribeiro. "Avaliação das práticas de gestão da cadeia de abastecimento sustentável das empresas portuguesas do setor têxtil e vestuário." Master's thesis, Instituto Superior de Economia e Gestão, 2019. http://hdl.handle.net/10400.5/19328.

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Mestrado em Gestão e Estratégia Industrial
O presente trabalho tem como objetivo avaliar as práticas de Gestão da Cadeia de Abastecimento Sustentável (GCAS) adotadas pelas empresas do Setor Têxtil e Vestuário (STV), bem como testar o eventual impacto que a adoção de tais práticas exerce no desempenho dessas empresas. A revisão de literatura permitiu a identificação de um instrumento de pesquisa, originalmente redigido na língua inglesa, o qual serviu como base para a realização do presente trabalho, após ter sido alvo de um processo de adaptação cultural e linguística. Este questionário foi remetido a 621 empresas, das quais 107 responderam (17,2%). A validade de construto foi apurada através da análise fatorial exploratória, da qual resultaram quatro construtos nas práticas de GCAS - práticas de gestão ambiental, integração da cadeia de abastecimento, práticas de inclusão social dos funcionários, e práticas de inclusão social da comunidade - e de outros quatro construtos no desempenho de GCAS ? competitividade, desempenho ambiental, desempenho social centrado nos funcionários, e desempenho social centrado na comunidade. Ambas as escalas exibem uma elevada fiabilidade, sendo o valor do alfa de Cronbach de 0,859 e 0,869, respetivamente. Através do estabelecimento de um score "próprio" para cada um dos construtos repartido em quartis, verifica-se que as empresas do STV têm vindo a adotar as práticas de GCAS a um nível suficiente, sendo igualmente suficiente o seu desempenho. A avaliação do coeficiente de correlação de Pearson permite concluir a existência de correlações positivas significativas entre as práticas de GCAS e o desempenho de GCAS.
The objective of this paper is to evaluate the Sustainable Supply Chain Management (GCAS) practices adopted by Textile and Clothing (STV) companies, as well as to test the possible impact of adopting such practices has on the performance of these companies. The literature review allowed the identification of a survey instrument, originally written in the English language, which served as the basis for the accomplishment of the present study, after having undergone a process of cultural and linguistic adaptation. This survey was sent to 621 companies, of which 107 answered (17.2%). Construct validity was assessed through exploratory factor analysis, resulting in four constructs in GCAS practices - environmental management practices, supply chain integration, employee social inclusion practices, and community social inclusion practices - and four other constructs on GCAS performance - competitiveness, environmental performance, employee-centered social performance, and community-centered social performance. Both scales exhibit high reliability, with Cronbach's alpha value of 0.859 and 0.869, respectively. Through the establishment an "own" score for each of the constructs divided into quartiles (bad, insufficient, sufficient and good), it is found that TCS companies have been adopting SSCM practices at a sufficient level, and their performance in the various dimensions of sustainability is also sufficient. Using a Pearson correlation coefficient, a correlation analysis was performed between the SSCM practices and the SSCM performance, which concluded that there were significant positive correlations.
info:eu-repo/semantics/publishedVersion

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Chakroun, Almahdi. "Rôle du facteur de transcription Otx2 dans le contrôle de la prolifération des précurseurs granulaires du cervelet et des médulloblastomes." Thesis, Université Côte d'Azur (ComUE), 2016. http://www.theses.fr/2016AZUR4114.

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Le facteur de transcription à homéodomaine Otx2 est essentiel au développement du système nerveux, et en particulier du cervelet, où il est exprimé dans les précurseurs des neurones granulaires (PCGs). Au cours du développement, les PCGs passent par des périodes prolifératives très intenses qui les exposent à la transformation tumorale. Ces cellules seraient ainsi à l’origine de la formation des cancers du cervelet, les médulloblastomes (MBs). Une altération génétique particulière est cependant retrouvée dans plus de 75% des MBs : la surexpression d’Otx2. Cette thèse vise à comprendre le rôle d’Otx2 dans le contrôle de la prolifération dans un contexte normal ou tumoral. D’abord, nous avons entrepris d’identifier et d’isoler ces précurseurs à partir de souriceaux, afin d’analyser leur caractéristiques prolifératives. Nos résultats ont montré que les cellules « Otx2+ » contiennent une fraction proliférative plus importante que les cellules « Otx2-». L’analyse du cycle cellulaire des cellules « Otx2+» montre également que ces dernières présentent des propriétés prolifératives distinctes. Ensuite, nous avons mis en oeuvre une approche de gain et de perte de fonction d’Otx2 dans une lignée de MBs exprimant Otx2 (HD-MB03). Nos résultats indiquent que la surexpression d’Otx2 stimule la prolifération de cette lignée, alors que sa perte de fonction la diminue. Enfin, Nous avons identifié par des analyses protéomiques par spectrométrie de masse des partenaires impliqués dans la régulation du cycle cellulaire, en particulier au niveau des phases S et G2M. Ces résultats suggèrent un mécanisme d’action unique d’Otx2 dans la régulation du cycle cellulaire dans le cervelet et les MBs
The homeobox transcription factor Otx2 is essential for the development of the central nervous system. During cerebellum development, Otx2 is expressed by granule cell precursors (GCPs), which have a high proliferation rate. Deregulation of GCPs proliferation may favor oncogenic processes, as seems to occur in medulloblastoma (MB), a malignant and invasive tumor of the cerebellum. A recurrent genetic alteration in medulloblastoma is the overexpression of Otx2 in 75% of the cases. The objective of this thesis is to study the role of Otx2 in the control of proliferation during normal and oncogenic development of the cerebellum. First, we investigated the role of Otx2 in the control of GCPs proliferation. Our results show that Otx2+ GCPs have an increased proliferation rate compared to «Otx2-» GCPs. In the second part of this work, we tested the oncogenic potential of Otx2 using the medulloblastoma cell line HD-MB03. We performed gain and loss of function experiments to analyze the effect of Otx2 expression on the proliferation of this cell line. Our results indicate that the overexpression of Otx2 increases the proliferation rate of HD-MB03 tumor cells. Conversely, Otx2 silencing significantly decreases it. Finally, to shed the light on the mechanism of action of Otx2 in the control of proliferation in cerebellum and medulloblastoma, we analyzed Otx2 protein partners in both cases by mass spectrometry analysis after immunoprecipating Otx2-protein complexes. We identified several protein partners that play an important role in cell cycle regulation, more specifically in S and G2M phases. Our project shows a pro-proliferative effect of Otx2 in cerebellum and medulloblastoma

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Schröder, Thomas [Verfasser], Hauke [Akademischer Betreuer] Lilie, Daniel [Akademischer Betreuer] Huster, and Karl-Wilhelm [Akademischer Betreuer] Koch. "Konformationsänderung des Guanylatzyklase-aktivierenden Proteins 2 (GCAP-2) zur Aktivierung der Sehstäbchenaußensegment-Guanylatzyklase (ROS-GC1) / Thomas Schröder. Betreuer: Hauke Lilie ; Daniel Huster ; Karl-Wilhelm Koch." Halle, Saale : Universitäts- und Landesbibliothek Sachsen-Anhalt, 2011. http://d-nb.info/102513513X/34.

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Wang, Chen-Wen, and 王貞雯. "Bioactive Effects and Potential Applications of GCAs Extracts." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/38299715498810110316.

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碩士
大仁科技大學
製藥科技研究所
103
Alpinia officinarum, Coronaribus Gingerlily, Alpinia zerumbet (GCAs) are commonly used herbal food supplements. They are widely cultivated and consumed throughout Taiwan. GCAs are full of volatile compounds consisting mainly of monoterpene (mainly limonene) hydrocarbons and exist especially in rhizomes or leaves. GCAs are generally recognized as aromatic and safe and have been used in various cuisine, and as food flavoring agents , liqueurs , beverages. GCAs have also been widely used as additives in cosmetic, perfumes, soaps, and articles for daily use. The major methods used for the assessment of bioactivity of GCAs have been summarized, which include in vitro and in vivo mucosal protection test, coronary artery dilatation test and the scavenging of DPPH free radical test. In this study, the effects of GCAs on mucosal cell growth were evaluated in vitro using a normal rat gastric mucosal cell line RGM-1. On the other hand, GCAs were also evaluated organ toxicity in vivo using ethanol-induced gastric mucosal damage model. GCAs produced significant bioactivity effect in the coronary artery dilatation and RGM-1 cell line test. No deterious effects on organs were observed.

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Venkatesan, Jagadeesh Kumar [Verfasser]. "Molecular and functional characterization of Ribeye-GCAP2 interaction in the photoreceptor ribbon synapse / Jagadeesh Kumar Venkatesan." 2010. http://d-nb.info/1011158906/34.

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Tao, Hongbin [Verfasser]. "Yield and nitrogen uptake of lowland rice (Oryza sativa L.) in a water-saving ground cover rice production system (GCRPS) in Beijing, North China / vorgelegt von Hongbin Tao." 2004. http://d-nb.info/971625190/34.

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Kreye, Christine [Verfasser]. "Greenhouse gas emissions (methane and nitrous oxide) and water use in a water-saving ground cover rice production system (GCRPS) in North China, Beijing / vorgelegt von Christine Kreye." 2004. http://d-nb.info/975516833/34.

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Mati, Jacob Mwathi. "Global civil society advocacy alliances and networks in the changing terrain of global governance and development : a critical inquiry into the politics and dynamics in crafting and operations of the Global Action against Poverty (GCAP)." Thesis, 2009. http://hdl.handle.net/10539/6102.

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The last few decades have witnessed the emergence of global civil society advocacy networks as major players in global governance. This research aimed at using a case study of GCAP in critically analysing the politics and dynamics of crafting a global civil society advocacy alliance. Specifically, the study aimed to: a) identify, analyse, and document GCAP’s experiences, strategies and challenges in trans-national networking and advocacy; b) generate knowledge on recent developments in global civil society networking and advocacy. The study analyses the study phenomenon using two central features of GCAP: networking and advocacy. Chapter one attempts to give a background of the study and also discusses the methods used. Chapter two lays the theoretical framework and operationalises the concepts explored in the study. The report argues that alliances are very different from ‘normal’ forms of organisations because they are made up of diverse forms of organisations, coming together voluntarily to achieve a specific purpose. They are therefore, by their very nature, complex, unstable, and difficult to co-ordinate. Chapters Three and Four look at such intricacies and complexities of crafting and operations of global advocacy networks. I conclude this research arguing that despite challenges in alliances building and operations, global civil society organisations will still need to network if they are to remain relevant and effective in current global governance context. It is only in their unity that they will be able to confront their common challenges.

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Книги з теми "GCAPs"

Commission, European, ed. GCAS operational requirements and certification: GORAC. Luxembourg: Office for Official Publications of the European Communities, 1998.

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J, Dent N., ed. Implementing international good practices: GAPs, GCPs, GLPs, GMPs. Buffalo Grove, IL: Interpharm Press, 1993.

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United States. National Aeronautics and Space Administration., ed. Trade study plan for graphite composite primary structure (GCPS). [Downey, Calif.]: Rockwell Aerospace, Space Systems Division, 1994.

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United States. National Aeronautics and Space Administration., ed. Test plans, GCPS task 7, subtask 7.1, IHM development. [Downey, Calif.]: Rockwell Aerospace, Space Systems Division, 1994.

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J, Foose Thomas, and IUCN/SSC Captive Breeding Specialist Group., eds. Rhino global captive action plan (GCAP): 1 September 1992. [S.l.]: IUCN/SSC Captive Breeding Specialist Group, 1992.

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United States. National Aeronautics and Space Administration., ed. Test plans, GCPS task 4, subtask 4.2, thrust structure development. [Downey, Calif.]: Rockwell Aerospace, Space Systems Division, 1994.

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United States. National Aeronautics and Space Administration., ed. Milestone 4: Thrust structure concepts & IHM screening graphite composite primary structure (GCPS). [Downey, Calif.]: Rockwell Aerospace, Space Systems Division, 1994.

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United States. National Aeronautics and Space Administration., ed. Structural arrangement trade study: Reusable hydrogen composite tank system and graphite composite primary structures (GCPS) : executive summary. [Washington, DC: National Aeronautics and Space Administration, 1995.

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United States. National Aeronautics and Space Administration., ed. Addendum to structural arrangement trade study: Reusable hydrogen composite tank system (RHCTS) and graphite composite primary structures (GCPS). [Washington, DC: National Aeronautics and Space Administration, 1995.

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Частини книг з теми "GCAPs"

Gorczyca, Wojciech A., and Izabela Sokal. "GCAPs: Ca2+-Sensitive Regulators of retGC." In Advances in Experimental Medicine and Biology, 319–32. Boston, MA: Springer US, 2002. http://dx.doi.org/10.1007/978-1-4615-0121-3_19.

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Koch, Karl-Wilhelm, and Jens Christoffers. "Mapping Calcium-Sensitive Regions in GCAPs by Site-Specific Fluorescence Labelling." In Methods in Molecular Biology, 583–94. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9030-6_36.

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Seal, U. S., T. J. Foose, and S. Ellis. "Conservation Assessment and Management Plans (CAMPs) and Global Captive Action Plans (GCAPs)." In Creative Conservation, 312–25. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0721-1_16.

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Dizhoor, Alexander. "Site-Directed and Natural Mutations in Studying Functional Domains in Guanylyl Cyclase Activating Proteins (GCAPs)." In Advances in Experimental Medicine and Biology, 291–301. Boston, MA: Springer US, 2002. http://dx.doi.org/10.1007/978-1-4615-0121-3_17.

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Anil Sethi, Anjula Garg, Jan Sacharko, Regina List, Jesse O. Bollinger, Lisiunia Romanienko, Allyson Reaves, David B. Howard, et al. "GCAP." In International Encyclopedia of Civil Society, 751. New York, NY: Springer US, 2010. http://dx.doi.org/10.1007/978-0-387-93996-4_9115.

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Baehr, Wolfgang, Iswari Subbaraya, Wojciech A. Gorczyca, and Krzysztof Palczewski. "Guanylate Cyclase-Activating Protein (GCAP)." In Degenerative Diseases of the Retina, 339–47. Boston, MA: Springer US, 1995. http://dx.doi.org/10.1007/978-1-4615-1897-6_38.

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Koch, Karl-Wilhelm. "GCAP (Guanylate Cyclase–Activating Protein)." In Encyclopedia of Signaling Molecules, 2041–45. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-67199-4_12.

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Koch, Karl-Wilhelm. "GCAP (Guanylate Cyclase–Activating Protein)." In Encyclopedia of Signaling Molecules, 1–5. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4614-6438-9_12-1.

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Meigs, Thomas E., Alex Lyakhovich, Hoon Shim, Ching-Kang Chen, Denis J. Dupré, Terence E. Hébert, Joe B. Blumer, et al. "GCAP (Guanylate Cyclase–Activating Protein)." In Encyclopedia of Signaling Molecules, 769–73. New York, NY: Springer New York, 2012. http://dx.doi.org/10.1007/978-1-4419-0461-4_12.

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Nabors, Laura, Filiberto Toledano-Toledano, and Brandon Workman. "Graded Chronic Pain Scale (GCPS)." In Handbook of Assessment in Mindfulness Research, 1–11. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-77644-2_65-1.

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Тези доповідей конференцій з теми "GCAPs"

Burns, Amy, Daniel Harper, Arthur F. Barfield, Shawn Whitcomb, and Brian Jurusik. "Auto GCAS for analog flight control system." In 2011 IEEE/AIAA 30th Digital Avionics Systems Conference (DASC). IEEE, 2011. http://dx.doi.org/10.1109/dasc.2011.6096148.

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Thomasson, J. Alex, Xiongzhe Han, G. C. Bagnall, Chao Sima, Yeyin Shi, William L. Rooney, Chenghai A. Yang, Jinha Jung, and Anjin Chang. "UAV data reliability improvements based on multifunctional GCPs." In Autonomous Air and Ground Sensing Systems for Agricultural Optimization and Phenotyping IV, edited by J. Alex Thomasson, Mac McKee, and Robert J. Moorhead. SPIE, 2019. http://dx.doi.org/10.1117/12.2518541.

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Goerke, Roland, and Werner Berger. "Tactical ground collision avoidance system T-GCAS®." In 2012 IEEE/AIAA 31st Digital Avionics Systems Conference (DASC). IEEE, 2012. http://dx.doi.org/10.1109/dasc.2012.6382953.

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Toutin, T., D. Hoja, E. Hoeppner, A. Remond, and C. King. "GCPs selection from multi-source data over mountainous topography." In IGARSS '98. Sensing and Managing the Environment. 1998 IEEE International Geoscience and Remote Sensing. Symposium Proceedings. (Cat. No.98CH36174). IEEE, 1998. http://dx.doi.org/10.1109/igarss.1998.702206.

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Goerke, R., and W. Berger. "A400M tactical ground collision avoidance system T-GCAS®." In 2012 IEEE/AIAA 31st Digital Avionics Systems Conference (DASC). IEEE, 2012. http://dx.doi.org/10.1109/dasc.2012.6382282.

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Jiao, Dian, Jiwei Hu, and Wanlin Wang. "A Method of Lunar Mapping SAR Baseline Estimation Without GCPs." In 2018 IEEE International Conference on Information and Automation (ICIA). IEEE, 2018. http://dx.doi.org/10.1109/icinfa.2018.8812525.

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Fan, Ning-jun, Ling-jun Zhao, Gang-yao Kuang, and Ying Cui. "Location of air-borne SAR imagery without GCPs and error analysis." In The International Conference on Remote Sensing,Environment and Transportation Engineering. Paris, France: Atlantis Press, 2013. http://dx.doi.org/10.2991/rsete.2013.3.

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Carpenter, James, Kenneth Gahan, and Richard Cobb. "Automatic-Ground Collision Avoidance System (Auto-GCAS) for Performance Limited Aircraft." In AIAA Aviation 2019 Forum. Reston, Virginia: American Institute of Aeronautics and Astronautics, 2019. http://dx.doi.org/10.2514/6.2019-3657.

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Hruska, Jonas, Luis Padua, Telmo Adao, Emanuel Peres, Jose Martinho, and Joaquim J. Sousa. "Target Influence on Ground Control Points (GCPs) Identification in Aerial Images." In IGARSS 2020 - 2020 IEEE International Geoscience and Remote Sensing Symposium. IEEE, 2020. http://dx.doi.org/10.1109/igarss39084.2020.9324222.

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Chen, Jun, Wei An, Xinpu Deng, Jungang Yang, and Zhichao Sha. "Space based optical staring sensor LOS determination and calibration using GCPs observation." In SPIE Security + Defence, edited by David A. Huckridge, Reinhard Ebert, and Stephen T. Lee. SPIE, 2016. http://dx.doi.org/10.1117/12.2240285.

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Звіти організацій з теми "GCAPs"

Toutin, Th, D. Hoja, E. Hoeppner, A. Rémond, and C. King. GCPs Selection from Multi-source Data Over Mountainous Topography. Natural Resources Canada/ESS/Scientific and Technical Publishing Services, 1998. http://dx.doi.org/10.4095/219387.

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Brodie, Katherine, Brittany Bruder, Richard Slocum, and Nicholas Spore. Simultaneous mapping of coastal topography and bathymetry from a lightweight multicamera UAS. Engineer Research and Development Center (U.S.), August 2021. http://dx.doi.org/10.21079/11681/41440.

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Анотація:

A low-cost multicamera Unmanned Aircraft System (UAS) is used to simultaneously estimate open-coast topography and bathymetry from a single longitudinal coastal flight. The UAS combines nadir and oblique imagery to create a wide field of view (FOV), which enables collection of mobile, long dwell timeseries of the littoral zone suitable for structure-from motion (SfM), and wave speed inversion algorithms. Resultant digital surface models (DSMs) compare well with terrestrial topographic lidar and bathymetric survey data at Duck, NC, USA, with root-mean-square error (RMSE)/bias of 0.26/–0.05 and 0.34/–0.05 m, respectively. Bathymetric data from another flight at Virginia Beach, VA, USA, demonstrates successful comparison (RMSE/bias of 0.17/0.06 m) in a secondary environment. UAS-derived engineering data products, total volume profiles and shoreline position, were congruent with those calculated from traditional topo-bathymetric surveys at Duck. Capturing both topography and bathymetry within a single flight, the presented multicamera system is more efficient than data acquisition with a single camera UAS; this advantage grows for longer stretches of coastline (10 km). Efficiency increases further with an on-board Global Navigation Satellite System–Inertial Navigation System (GNSS-INS) to eliminate ground control point (GCP) placement. The Appendix reprocesses the Virginia Beach flight with the GNSS–INS input and no GCPs.

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