Готові списки джерел за темами / Fluorescence properties

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Добірка наукової літератури з теми "Fluorescence properties"

Автор: Grafiati
Опубліковано: 20 квітня 2024

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Статті в журналах з теми "Fluorescence properties"

1

Li, Yanlei, Yi Cai, Zhe Yang, Haiyan Chen, Zhanying Zhang, Dongsheng Yu, Weikai Fang, Yanfei Wang, and Chidong Xu. "Comparison of the Fluorescence Properties of Biological Solutions and Aerosols." International Journal of Engineering and Technology 15, no. 3 (August 2023): 115–18. http://dx.doi.org/10.7763/ijet.2023.v15.1231.

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The identification of biological aerosols is a subject of worldwide interest because of the potential for biological terrorism and the increase in public health emergencies. Laser-induced fluorescence is widely used to investigate the fluorescence properties of biomolecules and spores in solution; however, data on the fluorescence properties of the aerosolized forms are lacking. In this study, we report the fluorescence properties of tryptophan, serotonin, tyrosine, vitamin B6, and Bacillus thuringiensis and Bacillus subtilis spores in solution and aerosol form. Our results show that the shapes of the fluorescence spectra were generally consistent between the solution and aerosol states despite some wavelength shifts in the emission spectra. In contrast, the fluorescence intensity of the emissions varied significantly between the solutions and aerosols. Given the growing global interest in accurate measurement of airborne biological molecules and particles, our findings provide a useful baseline for further research into the development of laser-induced fluorescence-based bioaerosol detection instruments and can guide the ongoing design of effective measurement and engineering technologies.
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2

Amteghy, Ali H. "Synthesis, Fluorescence and Thermal Properties of Some Benzidine Schiff Base." NeuroQuantology 20, no. 3 (March 26, 2022): 135–49. http://dx.doi.org/10.14704/nq.2022.20.3.nq22053.

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A series of Schiff bases derived from benzidine and various aromatic aldehydes were prepared and characterized by spectroscopic methods. Fluorescence properties of prepared Schiff bases were studied in DMF solution. The thermo kinetic parameters. E, Δ H, Δ S and Δ G were calculated following Coats-Redfern method.
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3

Das, Joydip, Rosalie K. Crouch, and Parkson Lee-Gau Chong. "Fluorescence Properties of Pyrylretinol." Photochemistry and Photobiology 72, no. 3 (May 1, 2007): 415–20. http://dx.doi.org/10.1562/0031-8655(2000)0720415fpop2.0.co2.

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4

Das, Joydip, Rosalie K. Crouch, and Parkson Lee-Gau Chong. "Fluorescence Properties of Pyrylretinol." Photochemistry and Photobiology 72, no. 3 (2000): 415. http://dx.doi.org/10.1562/0031-8655(2000)072<0415:fpop>2.0.co;2.

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5

Nakashima, Nobuaki, Stephen R. Meech, Andrew R. Auty, Anita C. Jones, and David Phillips. "Fluorescence properties of ergosterol." Journal of Photochemistry 30, no. 2 (August 1985): 207–14. http://dx.doi.org/10.1016/0047-2670(85)85025-5.

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6

Ren, Pan Pan, Ren Jie Wang, and Shou Zhi Pu. "Photochromism Properties of a New Unsymmetrical Diarylethene Bearing a Benzene Unit." Advanced Materials Research 830 (October 2013): 266–69. http://dx.doi.org/10.4028/www.scientific.net/amr.830.266.

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A new photochromic diarylethene compound 1-[(2-methyl-5-(4-trifluoromethyphenyl)-3-thienyl)]-2-{2-methyl-5-[2-(1,3-dioxolane)]-3-thienyl} perfluorocyclopentene 1O was synthesized. Its optoelectronic properties, such as photochromism in solution as well as in poly-methylmethacrylate (PMMA) amorphous films, fluorescences switch were investigated in detail. Upon irradiated UV light it changes from colorless to purple in solution and PMMA amorphous film, respectively. Finally, the diarylethene 1O was exhibited remarkable fluorescence switch properties along with the cyclization reactions. When the concentration arrived at 1 × 10-5 mol L-1, the fluorescence peak had the maximum.
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7

Chen Maofu, 陈茂福, 吴静 Wu Jing, 律严励 Lü Yanli, and 陈庆俊 Chen Qingjun. "Fluorescence Properties of Municipal Wastewater." Acta Optica Sinica 28, no. 3 (2008): 578–82. http://dx.doi.org/10.3788/aos20082803.0578.

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8

Leung, A. F., L. Hayashibara, and J. Spadaro. "Fluorescence properties of uranyl nitrates." Journal of Physics and Chemistry of Solids 60, no. 3 (March 1999): 299–304. http://dx.doi.org/10.1016/s0022-3697(98)00294-7.

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9

Nutiu, Razvan, and Yingfu Li. "Aptamers with fluorescence-signaling properties." Methods 37, no. 1 (September 2005): 16–25. http://dx.doi.org/10.1016/j.ymeth.2005.07.001.

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Anstead, Gregory M., and John A. Katzenellenbogen. "Fluorescence properties of 2,3-diarylindenes." Journal of Physical Chemistry 92, no. 22 (November 1988): 6249–58. http://dx.doi.org/10.1021/j100333a017.

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Дисертації з теми "Fluorescence properties"

1

Zhou, Xiaobo. "Design, synthesis and sensing properties of chiral amine-based fluorescent probes." HKBU Institutional Repository, 2012. https://repository.hkbu.edu.hk/etd_ra/1442.

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2

Ferguson, A. J. "Fluorescence properties of diphenylpolyenes in solution." Thesis, University of Nottingham, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.238201.

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3

Pauff, James. "Fluorescence properties of metallo-beta-lactamase L1." Miami University Honors Theses / OhioLINK, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1111003051.

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4

Yu, Yanhua. "Synthesis and properties of triazole-containing fluorescent molecules." Thesis, Cachan, Ecole normale supérieure, 2013. http://www.theses.fr/2013DENS0028.

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Cette thèse se concentre sur le design et la synthèse de molécules fluorescentes contenant un motif triazole et un squelette benzothiadiazole (BTD), coumarine, 4,4-difluoro-4-bora-3a,4a-diaza-s-indacène (BODIPY) ou dicyanométhylène-4H-pyran (DCM) par chimie "click" et l’étude de leurs propriétés et applications en biologie et en chimie analytique. Dans le but de synthétiser des peptides fluorescents et d'étudier leurs applications, des acides aminés fluorescents contenant BTD, coumarine et BODIPY ont été préparés par réaction "click", et incorporés dans la somatostatine par synthèse peptidique en phase solide. Les peptides fluorescents synthétisés pourront être utilisés pour le développement d'un test de "binding" des analogues de la somatostatine. Des dérivés de BTD et BODIPY ont également été conçus et synthétisés pour servir de mimes de coudes beta- qui conduisent à des peptides courts qui pourraient être facilement détectés et étudiés en utilisant des techniques de fluorescence. La capacité des composés obtenus à former des liaisons hydrogène intramoléculaires a été étudiée par spectroscopie infrarouge. En outre, une série de macrocycles à base de BODIPY contenant un C-glucopyranoside conjugué ou non à des acides aminés tels que glycine, acid aspartique ou méthionine ont été synthétisés avec succès en utilisant une réaction "click" comme étape de macrocyclisation. Certains des composés synthétisés présentent des propriétés de reconnaissance sélective vers Cu2+, Fe3+, F- et CN- dans l'acétonitrile. Enfin, un nouveau capteur fluorescent, qui est capable de reconnaître les cations et anions d'une manière coopérative, a été conçu et synthétisé par chimie "click". Ce composé est très sensible à des combinaisons de Cu2+, F- et / ou Br- d’une manière séquence- et halogénure-dépendante
This thesis is focused on the design and synthesis of triazole-containing fluorescent molecules based on benzothiadiazole (BTD), coumarin, 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) or dicyanomethylene-4H-pyran (DCM) by “click” chemistry and investigation of their properties and applications in biology and analytical chemistry. In the aim to synthesize fluorescent peptides and investigate their applications, fluorescent amino acids containing BTD, coumarin and BODIPY were prepared by “click” reaction, and incorporated into somatostatin through solid phase peptide synthesis. The resulting fluorescent peptides could be used for the development of a binding assay for somatostatin analogues. BTD and BODIPY derivatives have also been designed and synthesized to act as beta-turn mimics which lead to short conformationally restricted peptides that could be easily detected and studied using fluorescence techniques. The ability of the synthesized compounds to form intramolecular hydrogen bond was studied by infrared spectroscopy. Moreover, a series of BODIPY-based macrocycles containing a C-glucopyranoside conjugated or not with various amino acids such as glycine, aspartic acid or methionine have been successfully synthesized by using “click” reaction as the macrocyclization step. Some of the synthesized compounds exhibited selective recognition properties towards Cu2+, Fe3+, F- and CN- in acetonitrile. Finally, a new fluorescent sensor, which has the ability to recognize cations and anions in a cooperative way, was designed and synthesized by “click” chemistry. This compound was highly sensitive to combinations of Cu2+, F– and/or Br– in a sequence- and halide-dependent way
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5

Manceau, Mathieu. "Single CdSe/CdS dot-in-rods fluorescence properties." Thesis, Paris 6, 2014. http://www.theses.fr/2014PA066362/document.

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Les nanocristaux colloïdaux synthétisés par voie chimique sont des sources prometteuses de lumière non-Classique à température ambiante. Ce travail est consacré à l'étude des propriétés optiques d'un type particulier de nanocristaux colloïdaux, appelé coeur-Bâtonnet, dans lequel un noyau de Seleniure de Cadmium (CdSe) sphérique est entouré d'une coquille de Sulfure de Cadmium (CdS) de forme cylindrique. En étudiant des particules de type coeur-Bâtonnet à température ambiante avec un microscope confocal, une caractérisation complète des propriétés optiques de ces émetteurs est réalisée. Nous étudions d'abord la statistique de clignotement de ces émetteurs. Nous montrons que les émetteurs coeur-Bâtonnet avec des coquilles épaisses se caractérisent par un clignotement réduit sur des échelles de temps courts, inférieurs à quelques millisecondes. Ensuite, une caractérisation détaillée de la statistique de photons des émetteurs coeur-Bâtonnet tenant compte du phénomène de clignotement est réalisée. La polarisation de l'émission est également étudié. Nous montrons que la polarisation d'émission peut être controlée en changeant la géométrie de la structure.Enfin, nous présentons également des expériences de couplage de ces émetteurs avec des dispositifs photoniques. Nous montrons la possibilité d'exciter un émetteur coeur-Bâtonnet en utilisant un nanofil d'oxyde de Zinc (ZnO). Nous montrons aussi que nous sommes en mesure d'orienter efficacement des nanoémetteurs uniques en utilisant la formation controlée de défauts dans des cristaux liquides
Wet-Chemically synthesized colloidal nanocrystals are promising room temperature non-Classical light sources. This work is devoted to the study of the optical properties of a particular type of colloidal nanocrystals, called dot-In-Rods, in which a spherical Cadmium Selenide (CdSe) core is surrounded by a rod-Like Cadmium Sulfide (CdS) shell. By studying single dot-In-Rods at room-Temperature with a confocal microscope, a complete characterization of the optical, and especially quantum optical, properties of dot-In-Rods is provided for several geometrical parameters. We first study the blinking statistics of such emitters. We show that dot-In-Rods with thick shells are characterized by a reduced blinking that happens on fast timescales, typically on millisecond timescales. We then go on with a detailed characterization of the photon statistics of dot-In-Rods. A complete description of the photon statistics taking into account the blinking process is realized. The polarization of the emission is also investigated. We show that the emission polarization can be tuned by engineering the geometry. Finally, we also present experiments where we couple dot-In-Rods with various photonic devices. We demonstrate the possibility of excitation of a single emitter using a Zinc Oxyde (ZnO) nanowire. Using defects in liquid crystals, we also show that we are able to efficiently orientate single nanoemitters
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6

Lutkouskaya, Katsiaryna. "Fluorescence properties of dye-zeolite L nanochannel materials /." [S.l.] : [s.n.], 2007. http://www.zb.unibe.ch/download/eldiss/07lutkouskaya_k.pdf.

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7

Vall-Llosera, Gemma. "Fluorescence properties of trendy molecules studied with synchrotron radiation /." Stockholm : School of Engineering Sciences, Royal Institute of Technology, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4148.

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8

Grazios, Fabio. "Fluorescence properties of single nitrogen-vacancy centre in diamond." Thesis, University of Oxford, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.543481.

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9

Ismail, G. H. "Studies on the fluorescence and photoyellowing properties of wool." Thesis, City University London, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.378863.

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10

Jazmati, A. K. "Optical properties and laser induced fluorescence from BGO:RE waveguides." Thesis, University of Sussex, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297953.

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Книги з теми "Fluorescence properties"

1

Haunton, Kathleen Mary. The fluorescence properties of irradiated polymers. Uxbridge: Brunel University, 1992.

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2

N, Berberan-Santos M., ed. Fluorescence of supermolecules, polymers, and nanosystems. Berlin: Springer, 2008.

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3

Simpson, W. S. Fluorescence properties of wool and other animal fibres. Christchurch: Wronz, 1992.

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4

Berberan-Santos, Mario N., and Alberto U. Acun a. Fluorescence of supermolecules, polymers, and nanosystems. Berlin: Springer, 2010.

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5

V, Tuchin V., European Optical Society, Society of Photo-optical Instrumentation Engineers., European Laser Association, and Netherlands Medical Laser Association, eds. Controlling tissue optical properties: Applications in clinical study : 5-6 July 2000, Amsterdam, Netherlands. Bellingham, Wash., USA: SPIE, 2000.

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6

J, Bigio Irving, European Optical Society, Society of Photo-optical Instrumentation Engineers., European Laser Association, and Netherlands Medical Laser Association, eds. Optical biopsy and tissue optics: 5-6 July 2000, Amsterdam, Netherlands. Bellingham, Wash., USA: SPIE, 2000.

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7

Kubicki, Jacek. Spektralne i fotofizyczne właściwości wzbudzonych cząsteczek i krótko żyjących indywiduów przejściowych na przykładzie tioketonów i zasad Schiffa: Spectral and photophysical properties of tioketones and Schiff bases : excited molecules and transient species. Poznań: Wydawnictwo Naukowe Uniwersytetu im. Adama Mickiewicza, 2008.

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8

United States. National Aeronautics and Space Administration., ed. Bio-optical properties of the Arabian Sea as determined by in situ and Sea WiFS data: Final report prepared under NASA grant NAGW-2154, 1 February 1993 - 30 April 1997. San Diego, CA: CHORS, Center for Hydro-Optics & Remote Sensing, San Diego State University, 1997.

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9

United States. National Aeronautics and Space Administration., ed. Bio-optical properties of the Arabian Sea as determined by in situ and Sea WiFS data: Final report prepared under NASA grant NAGW-2154, 1 February 1993 - 30 April 1997. San Diego, CA: CHORS, Center for Hydro-Optics & Remote Sensing, San Diego State University, 1997.

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10

1941-, Alfano Robert R., International Biomedical Optics Society, and Society of Photo-optical Instrumentation Engineers., eds. Optical biopsy III: 23-24 January 2000, San Jose, California. Bellingham, Wash., USA: SPIE, 2000.

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Частини книг з теми "Fluorescence properties"

1

Ohtsubo, Seiji, and Mitsuo Kusano. "Indocyanine Green Fluorescence Properties." In ICG Fluorescence Imaging and Navigation Surgery, 9–20. Tokyo: Springer Japan, 2016. http://dx.doi.org/10.1007/978-4-431-55528-5_2.

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2

Zhou, Bing, Yi Lin, Barbara A. Harruff, and Ya-Ping Sun. "Photoluminescence Properties of Carbon Nanotubes." In Springer Series on Fluorescence, 363–80. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/4243_2007_011.

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3

Stöckl, Martin T., Ranieri Bizzarri, and Vinod Subramaniam. "Studying Membrane Properties Using Fluorescence Lifetime Imaging Microscopy (FLIM)." In Springer Series on Fluorescence, 215–40. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/4243_2012_48.

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4

Alfimov, M. V., and S. P. Gromov. "Fluorescence Properties of Crown-Containing Molecules." In Applied Fluorescence in Chemistry, Biology and Medicine, 161–78. Berlin, Heidelberg: Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-59903-3_8.

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5

Loseva, Elizaveta, Jaap van Krugten, Aniruddha Mitra, and Erwin J. G. Peterman. "Single-Molecule Fluorescence Microscopy in Sensory Cilia of Living Caenorhabditis elegans." In Single Molecule Analysis, 133–50. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3377-9_7.

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AbstractIntracellular transport of organelles and biomolecules is vital for several cellular processes. Single-molecule fluorescence microscopy can illuminate molecular aspects of the dynamics of individual biomolecules that remain unresolved in ensemble experiments. For example, studying single-molecule trajectories of moving biomolecules can reveal motility properties such as velocity, diffusivity, location and duration of pauses, etc. We use single-molecule imaging to study the dynamics of microtubule-based motor proteins and their cargo in the primary cilia of living C. elegans. To this end, we employ standard fluorescent proteins, an epi-illuminated, widefield fluorescence microscope, and primarily open-source software. This chapter describes the setup we use, the preparation of samples, a protocol for single-molecule imaging in primary cilia of C. elegans, and data analysis.
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6

Berberan-Santos, Mário N., Evgeny N. Bodunov, and Bernard Valeur. "Luminescence Decays with Underlying Distributions of Rate Constants: General Properties and Selected Cases." In Springer Series on Fluorescence, 67–103. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/4243_2007_001.

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7

Bagatolli, L. A. "LAURDAN Fluorescence Properties in Membranes: A Journey from the Fluorometer to the Microscope." In Springer Series on Fluorescence, 3–35. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/4243_2012_42.

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Bower, D. I. "Infrared dichroism, polarized fluorescence and Raman spectroscopy." In Structure and Properties of Oriented Polymers, 181–233. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5844-2_4.

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Gonnord, Pauline, and Rajat Varma. "Unveiling Biophysical and Biological Properties of a Hypothetical Membrane Receptor by Exploiting Recent Imaging Advances." In Springer Series on Fluorescence, 367–88. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/4243_2012_53.

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10

Szigeti, Z., E. Pölös, and E. Lehoczki. "Fluorescence Properties of Paraquat Resistant Conyza Leaves." In Applications of Chlorophyll Fluorescence in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing, 109–14. Dordrecht: Springer Netherlands, 1988. http://dx.doi.org/10.1007/978-94-009-2823-7_13.

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Тези доповідей конференцій з теми "Fluorescence properties"

1

Maruoka, Shoji, Yohei Mitsui, Shinpei Okawa, Yoko Hoshi, and Yukio Yamada. "Measurement of Fluorescence Properties in Light Scattering Medium." In ASME/JSME 2011 8th Thermal Engineering Joint Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/ajtec2011-44458.

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The purpose of this study is to measure the fluorescence properties of Indocyaninegreen (ICG) which is a fluorescence dye to be used as a fluorescence probe for the use of fluorescence imaging in biomedical applications. The fluorescence molecular imaging is expected to solve the issues in preclinical studies which require a lot of time, labors and sacrificed animals. Information of living body can be obtained by measuring the fluorescent properties of the probe in biological media. The absorption and emission spectra and the lifetime of ICG in non-scattering and scattering media were measured in this study. ICG was dissolved in water, in plasma, in Intralipid, and in a mixture of plasma and Intralipid to simulate the environment in living tissues. The absorption and emission spectra were measured using a fluorescence spectrophotometer. The fluorescence lifetimes were measured using a time-resolved measurement method. Results suggest that the fluorescent properties are affected by the reaction between ICG and biological tissues.
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2

Pushkarevskaya, A. A., A. V. Aralov, and A. A. Lomzov. "HYBRIDIZATION AND FLUORESCENCE PROPERTIES OF MODIFIED OLIGODEOXYRIBONUCLEOTIDES CONTAINING A N6-MODIFIED ADENINE." In X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-207.

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The properties of oligodeoxyribonucleotides containing an analog of N6-modified adenine studied. The thermodynamic parameters of duplexes formation containing a fluorescent nucleoside have been determined, and the fluorescence quantum yield of a nucleotide, oligonucleotides and duplexes containing it were determined. The potential of using such oligomers as fluorescent probes has been demonstrated.
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Corp, Lawrence A., James E. McMurtrey III, Moon S. Kim, Craig S. T. Daughtry, Emmett W. Chappelle, and Charles L. Mulchi. "Physical properties of leaf level fluorescence." In AeroSense '97, edited by Ram M. Narayanan and James E. Kalshoven, Jr. SPIE, 1997. http://dx.doi.org/10.1117/12.277616.

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Kane, C. L. "Electron Interactions and Excitons in Carbon Nanotube Fluorescence Spectroscopy." In ELECTRIC PROPERTIES OF SYNTHETIC NANOSTRUCTURES: XVII International Winterschool/Euroconference on Electronic Properties of Novel Materials. AIP, 2004. http://dx.doi.org/10.1063/1.1812117.

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Piramidowicz, R., A. Jusza, K. Anders, J. D. Fidelus, M. Godlewski, and S. Yatsunenko. "Fluorescence properties of Pr:YSZ Doped polymer nanocomposites." In 12th European Quantum Electronics Conference CLEO EUROPE/EQEC. IEEE, 2011. http://dx.doi.org/10.1109/cleoe.2011.5942836.

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Porque, Jerome, Shibin Jiang, Bor-Chyuan Hwang, Vladimir Fuflyigin, Edward J. Salley, Jing Zhao, and Nasser Peyghambarian. "Fluorescence properties of erbium-doped germanate glasses." In Symposium on Integrated Optoelectronics, edited by Shibin Jiang. SPIE, 2000. http://dx.doi.org/10.1117/12.382880.

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Mazurak, Zbigniew, K. Gornik, and D. Sek. "Fluorescence properties of the Schiff-base polymers." In 2000 International Conference on Application of Photonic Technology (ICAPT 2000), edited by Roger A. Lessard and George A. Lampropoulos. SPIE, 2000. http://dx.doi.org/10.1117/12.406464.

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Carbonaro, Carlo M., Riccardo Corpino, Pier Carlo Ricci, Daniele Chiriu, and Carla Cannas. "Fluorescence properties of dye doped mesoporous silica." In FUNDAMENTALS AND APPLICATIONS IN SILICA AND ADVANCED DIELECTRICS (SIO2014): X International Symposium on SiO2, Advanced Dielectrics and Related Devices. AIP Publishing LLC, 2014. http://dx.doi.org/10.1063/1.4900452.

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Huang, C. Q., Q. Cai, and W. J. Tian. "Fluorescence properties of RE-doped silica spheres." In Photonics Asia 2010, edited by Xuping Zhang, Hai Ming, and Alan Xiaolong Wang. SPIE, 2010. http://dx.doi.org/10.1117/12.870593.

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Mele, E. J. "Excitons in Carbon Nanotube Fluorescence Spectroscopy." In MOLECULAR NANOSTRUCTURES: XVII International Winterschool Euroconference on Electronic Properties of Novel Materials. AIP, 2003. http://dx.doi.org/10.1063/1.1628072.

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Звіти організацій з теми "Fluorescence properties"

1

Strand, Michael P. Coastal Benthic Optical Properties Fluorescence Imaging Laser Line Scan Sensor. Fort Belvoir, VA: Defense Technical Information Center, September 2002. http://dx.doi.org/10.21236/ada628584.

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2

Mazel, Charles. In Situ Spectral Properties (Reflectance and Fluorescence) of Benthic Substrates and Organisms. Fort Belvoir, VA: Defense Technical Information Center, September 1997. http://dx.doi.org/10.21236/ada628810.

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3

Sanamyan, Tigran, Sudhir Trivedi, and Mark Dubinskii. Fluorescence Properties of Fe2+- and Co2+-doped Hosts of CdMnTe Compositions as Potential Mid-Infrared Laser Materials. Fort Belvoir, VA: Defense Technical Information Center, September 2011. http://dx.doi.org/10.21236/ada553046.

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Baral, Aniruddha, Jeffery Roesler, and Junryu Fu. Early-age Properties of High-volume Fly Ash Concrete Mixes for Pavement: Volume 2. Illinois Center for Transportation, September 2021. http://dx.doi.org/10.36501/0197-9191/21-031.

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High-volume fly ash concrete (HVFAC) is more cost-efficient, sustainable, and durable than conventional concrete. This report presents a state-of-the-art review of HVFAC properties and different fly ash characterization methods. The main challenges identified for HVFAC for pavements are its early-age properties such as air entrainment, setting time, and strength gain, which are the focus of this research. Five fly ash sources in Illinois have been repeatedly characterized through x-ray diffraction, x-ray fluorescence, and laser diffraction over time. The fly ash oxide compositions from the same source but different quarterly samples were overall consistent with most variations observed in SO3 and MgO content. The minerals present in various fly ash sources were similar over multiple quarters, with the mineral content varying. The types of carbon present in the fly ash were also characterized through x-ray photoelectron spectroscopy, loss on ignition, and foam index tests. A new computer vision–based digital foam index test was developed to automatically capture and quantify a video of the foam layer for better operator and laboratory reliability. The heat of hydration and setting times of HVFAC mixes for different cement and fly ash sources as well as chemical admixtures were investigated using an isothermal calorimeter. Class C HVFAC mixes had a higher sulfate imbalance than Class F mixes. The addition of chemical admixtures (both PCE- and lignosulfonate-based) delayed the hydration, with the delay higher for the PCE-based admixture. Both micro- and nano-limestone replacement were successful in accelerating the setting times, with nano-limestone being more effective than micro-limestone. A field test section constructed of HVFAC showed the feasibility and importance of using the noncontact ultrasound device to measure the final setting time as well as determine the saw-cutting time. Moreover, field implementation of the maturity method based on wireless thermal sensors demonstrated its viability for early opening strength, and only a few sensors with pavement depth are needed to estimate the field maturity.
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Olson, L. C., R. D. Knight, H L Crow, and H. A. J. Russell. Chemostratigraphic logging of the Lower Ordovician and Precambrian, Bells Corners borehole calibration facility, Ottawa, Ontario. Natural Resources Canada/CMSS/Information Management, 2022. http://dx.doi.org/10.4095/330519.

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Geochemical data were collected from a 120-meter-deep borehole (BC81-2) located at the Geological Survey of Canada's Bells Corners Borehole Calibration Facility in Ottawa, Ontario. This report documents geochemical data collected for the most frequently logged of the six boreholes located at the facility. The geochemical data were collected using a portable X-ray fluorescence (pXRF) spectrometer with a subset of samples collected for modern laboratory based fusion and multi-acid methods to calibrate the pXRF data for improved accuracy. Borehole geochemistry provides a characterization of Precambrian and overlying Ordovician rocks in the Ottawa area that augments the understanding of geophysical properties obtained from the calibration borehole. The combined geochemical and geophysical datasets further define rock characteristics, formational boundaries, as well as alteration zones. The results demonstrate the usefulness of pXRF derived geochemical data and chemostratigraphy of rock cores. The chemostratigraphic data augment data collected with spectral gamma logging tools and support an enhanced interpretation of geological contacts across transitional boundaries and the nature of the matrix mineralogy.
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Choudhary, Ruplal, Victor Rodov, Punit Kohli, John D. Haddock, and Samir Droby. Antimicrobial and antioxidant functionalized nanoparticles for enhancing food safety and quality: proof of concept. United States Department of Agriculture, September 2012. http://dx.doi.org/10.32747/2012.7597912.bard.

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General concept. The reported 1-year study tested the feasibility ofpreparing antimicrobial and antioxidant nanoparticlesfunctionalized with natural phenolic compounds, as a first step to reach the ultimate goal - improving safely and quality of foods by developing novel antimicrobial and antioxidant food-contacting materials. The secondary objectives of the study were (a) selecting the most promising phenoliccompounds, (b) building nanoparticles with the selected phenolicgrafted on their Surface, and (c) testing antimicrobial and antioxidant properties of these particles. The study was expected to provide a " go/no go" decision as concerning the prospects of phenolic- bound nanoparticles as antimicrobial and antioxidant agents. Results. In course of the feasibility study, curucminwas chosen as the most promising phenoliccompound due to its high antibacterial activity exceeding other tested compounds by at leas one order of magnitude. Lipsome-typephospholipid/polydiacetylene(PDA) nanoparticlesfunctionalizedwith curcuminwere successfully built. The pitfall of limited curcumin amount that could be covalently bound to theparticle surface was circumvented by inclusion of curcunun in the liposome body. It was suggested onthe basis of fluorescence spectroscopy that curcuminwas bound by hydrophobic forces in the bi1ayer periphery of the Liposomesand therefore mightexert a contact effect on microorganisms. The curcumin­ functionalizednanoparticles(CFN) were shown to have a strong bactericidal activity towards both Gram-negative (E. coli) and Gram-positive (B. ce,·e11s) bacteria, but only limited effect against yeast. Furthermore, beyond the originallyplanned objectives, preliminary trials showed that CFN could be bound to silanized glass surface rendering aנבtiנnicrobial activity to the glass. Tnaddition, the particles showed antioxidantcapacity. Tberefore, it ,vas co11cluded tlוattlוeaims of tlוefeasibility study bad been successfully reached an
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Epel, Bernard L., Roger N. Beachy, A. Katz, G. Kotlinzky, M. Erlanger, A. Yahalom, M. Erlanger, and J. Szecsi. Isolation and Characterization of Plasmodesmata Components by Association with Tobacco Mosaic Virus Movement Proteins Fused with the Green Fluorescent Protein from Aequorea victoria. United States Department of Agriculture, September 1999. http://dx.doi.org/10.32747/1999.7573996.bard.

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The coordination and regulation of growth and development in multicellular organisms is dependent, in part, on the controlled short and long-distance transport of signaling molecule: In plants, symplastic communication is provided by trans-wall co-axial membranous tunnels termed plasmodesmata (Pd). Plant viruses spread cell-to-cell by altering Pd. This movement scenario necessitates a targeting mechanism that delivers the virus to a Pd and a transport mechanism to move the virion or viral nucleic acid through the Pd channel. The identity of host proteins with which MP interacts, the mechanism of the targeting of the MP to the Pd and biochemical information on how Pd are alter are questions which have been dealt with during this BARD project. The research objectives of the two labs were to continue their biochemical, cellular and molecular studies of Pd composition and function by employing infectious modified clones of TMV in which MP is fused with GFP. We examined Pd composition, and studied the intra- and intercellular targeting mechanism of MP during the infection cycle. Most of the goals we set for ourselves were met. The Israeli PI and collaborators (Oparka et al., 1999) demonstrated that Pd permeability is under developmental control, that Pd in sink tissues indiscriminately traffic proteins of sizes of up to 50 kDa and that during the sink to source transition there is a substantial decrease in Pd permeability. It was shown that companion cells in source phloem tissue export proteins which traffic in phloem and which unload in sink tissue and move cell to cell. The TAU group employing MP:GFP as a fluorescence probe for optimized the procedure for Pd isolation. At least two proteins kinases found to be associated with Pd isolated from source leaves of N. benthamiana, one being a calcium dependent protein kinase. A number of proteins were microsequenced and identified. Polyclonal antibodies were generated against proteins in a purified Pd fraction. A T-7 phage display library was created and used to "biopan" for Pd genes using these antibodies. Selected isolates are being sequenced. The TAU group also examined whether the subcellular targeting of MP:GFP was dependent on processes that occurred only in the presence of the virus or whether targeting was a property indigenous to MP. Mutant non-functional movement proteins were also employed to study partial reactions. Subcellular targeting and movement were shown to be properties indigenous to MP and that these processes do not require other viral elements. The data also suggest post-translational modification of MP is required before the MP can move cell to cell. The USA group monitored the development of the infection and local movement of TMV in N. benthamiana, using viral constructs expressing GFP either fused to the MP of TMV or expressing GFP as a free protein. The fusion protein and/or the free GFP were expressed from either the movement protein subgenomic promoter or from the subgenomic promoter of the coat protein. Observations supported the hypothesis that expression from the cp sgp is regulated differently than expression from the mp sgp (Szecsi et al., 1999). Using immunocytochemistry and electron microscopy, it was determined that paired wall-appressed bodies behind the leading edge of the fluorescent ring induced by TMV-(mp)-MP:GFP contain MP:GFP and the viral replicase. These data suggest that viral spread may be a consequence of the replication process. Observation point out that expression of proteins from the mp sgp is temporary regulated, and degradation of the proteins occurs rapidly or more slowly, depending on protein stability. It is suggested that the MP contains an external degradation signal that contributes to rapid degradation of the protein even if expressed from the constitutive cp sgp. Experiments conducted to determine whether the degradation of GFP and MP:GFP was regulated at the protein or RNA level, indicated that regulation was at the protein level. RNA accumulation in infected protoplast was not always in correlation with protein accumulation, indicating that other mechanisms together with RNA production determine the final intensity and stability of the fluorescent proteins.
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8

Steffens, John C., and Eithan Harel. Polyphenol Oxidases- Expression, Assembly and Function. United States Department of Agriculture, January 1995. http://dx.doi.org/10.32747/1995.7571358.bard.

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Polyphenol oxidases (PPOs) participate in the preparation of many plant products on the one hand and cause considerable losses during processing of plant products on the other hand. However, the physiological functions of plant PPO were still a subject of controversy at the onset of the project. Preliminary observations that suggested involvement of PPOs in resistance to herbivores and pathogens held great promise for application in agriculture but required elucidation of PPO's function if modulation of PPO expression is to be considered for improving plant protection or storage and processing of plant products. Suggestions on a possible role of PPO in various aspects of chloroplast metabolism were also relevant in this context. The characterization of plant PPO genes opened a way for achieving these goals. We reasoned that "understanding PPO targeting and routing, designing ways to manipulate its expression and assessing the effects of such modifications will enable determination of the true properties of the enzyme and open the way for controlling its activity". The objective of the project was to "obtain an insight into the function and biological significance of PPOs" by examining possible function(s) of PPO in photosynthesis and plant-pest interactions using transgenic tomato plants; extending our understanding of PPO routing and assembly and the mechanism of its thylakoid translocation; preparing recombinant PPOs for use in import studies, determination of the genuine properties of PPOs and understanding its assembly and determining the effect of PPO's absence on chloroplast performance. Results obtained during work on the project made it necessary to abandon some minor objectives and devote the effort to more promising topics. Such changes are mentioned in the 'Body of the report' which is arranged according to the objectives of the original proposal. The complex expression pattern of tomato PPO gene family was determined. Individual members of the family are differentially expressed in various parts of the plant and subjected to developmentally regulated turnover. Some members are differentially regulated also by pathogens, wounding and chemical wound signals. Wounding systemically induces PPO activity and level in potato. Only tissues that are developmentally competent to express PPO are capable of responding to the systemic wounding signal by increased accumulation of PPO mRNA. Down regulation of PPO genes causes hyper susceptibility to leaf pathogens in tomato while over expression regulation of PPO expression in tomato plants is their apparent increased tolerance to drought. Both the enhanced disease resistance conferred by PPO over expression and the increased stress tolerance due to down regulation can be used in the engineering of improved crop plants. Photosynthesis rate and variable fluorescence measurements in wild type, and PPO-null and over expressing transgenic tomato lines suggest that PPO does not enable plants to cope better with stressful high light intensities or reactive oxygen species. Rather high levels of the enzyme aggravate the damage caused under such conditions. Our work suggests that PPO's primary role is in defending plants against pathogens and herbivores. Jasmonate and ethylene, and apparently also salicylate, signals involved in responses to wounding and defense against herbivores and pathogens, enhance markedly and specifically the competence of chloroplasts to import and process pPPO. The interaction of the precursor with thylakoid membranes is primarily affected. The routing of PPO shows other unusual properties: stromal processing occurs in two sites, resulting in intermediates that are translocated across thylakoids by two different mechanisms - a DpH- and a Sec-dependent one. It is suggested that the dual pattern of processing and routing constitutes a'fail safe' mechanism, reflecting the need for a rapid and flexible response to defense challenges. Many of the observations described above should be taken into consideration when manipulation of PPO expression is contemplated for use in crop improvement.
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Engel, Bernard, Yael Edan, James Simon, Hanoch Pasternak, and Shimon Edelman. Neural Networks for Quality Sorting of Agricultural Produce. United States Department of Agriculture, July 1996. http://dx.doi.org/10.32747/1996.7613033.bard.

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The objectives of this project were to develop procedures and models, based on neural networks, for quality sorting of agricultural produce. Two research teams, one in Purdue University and the other in Israel, coordinated their research efforts on different aspects of each objective utilizing both melons and tomatoes as case studies. At Purdue: An expert system was developed to measure variances in human grading. Data were acquired from eight sensors: vision, two firmness sensors (destructive and nondestructive), chlorophyll from fluorescence, color sensor, electronic sniffer for odor detection, refractometer and a scale (mass). Data were analyzed and provided input for five classification models. Chlorophyll from fluorescence was found to give the best estimation for ripeness stage while the combination of machine vision and firmness from impact performed best for quality sorting. A new algorithm was developed to estimate and minimize training size for supervised classification. A new criteria was established to choose a training set such that a recurrent auto-associative memory neural network is stabilized. Moreover, this method provides for rapid and accurate updating of the classifier over growing seasons, production environments and cultivars. Different classification approaches (parametric and non-parametric) for grading were examined. Statistical methods were found to be as accurate as neural networks in grading. Classification models by voting did not enhance the classification significantly. A hybrid model that incorporated heuristic rules and either a numerical classifier or neural network was found to be superior in classification accuracy with half the required processing of solely the numerical classifier or neural network. In Israel: A multi-sensing approach utilizing non-destructive sensors was developed. Shape, color, stem identification, surface defects and bruises were measured using a color image processing system. Flavor parameters (sugar, acidity, volatiles) and ripeness were measured using a near-infrared system and an electronic sniffer. Mechanical properties were measured using three sensors: drop impact, resonance frequency and cyclic deformation. Classification algorithms for quality sorting of fruit based on multi-sensory data were developed and implemented. The algorithms included a dynamic artificial neural network, a back propagation neural network and multiple linear regression. Results indicated that classification based on multiple sensors may be applied in real-time sorting and can improve overall classification. Advanced image processing algorithms were developed for shape determination, bruise and stem identification and general color and color homogeneity. An unsupervised method was developed to extract necessary vision features. The primary advantage of the algorithms developed is their ability to learn to determine the visual quality of almost any fruit or vegetable with no need for specific modification and no a-priori knowledge. Moreover, since there is no assumption as to the type of blemish to be characterized, the algorithm is capable of distinguishing between stems and bruises. This enables sorting of fruit without knowing the fruits' orientation. A new algorithm for on-line clustering of data was developed. The algorithm's adaptability is designed to overcome some of the difficulties encountered when incrementally clustering sparse data and preserves information even with memory constraints. Large quantities of data (many images) of high dimensionality (due to multiple sensors) and new information arriving incrementally (a function of the temporal dynamics of any natural process) can now be processed. Furhermore, since the learning is done on-line, it can be implemented in real-time. The methodology developed was tested to determine external quality of tomatoes based on visual information. An improved model for color sorting which is stable and does not require recalibration for each season was developed for color determination. Excellent classification results were obtained for both color and firmness classification. Results indicted that maturity classification can be obtained using a drop-impact and a vision sensor in order to predict the storability and marketing of harvested fruits. In conclusion: We have been able to define quantitatively the critical parameters in the quality sorting and grading of both fresh market cantaloupes and tomatoes. We have been able to accomplish this using nondestructive measurements and in a manner consistent with expert human grading and in accordance with market acceptance. This research constructed and used large databases of both commodities, for comparative evaluation and optimization of expert system, statistical and/or neural network models. The models developed in this research were successfully tested, and should be applicable to a wide range of other fruits and vegetables. These findings are valuable for the development of on-line grading and sorting of agricultural produce through the incorporation of multiple measurement inputs that rapidly define quality in an automated manner, and in a manner consistent with the human graders and inspectors.
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Lesser, Michael P. Coastal Benthic Optical Properties (CoBOP) of Coral Reef Environments: Small Scale Fluorescent Optical Signatures and Hyperspectral Remote Sensing of Coral Reef Habitats. Fort Belvoir, VA: Defense Technical Information Center, September 2001. http://dx.doi.org/10.21236/ada627969.

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