Готові списки джерел за темами / Cytochemistry

Добірка наукової літератури з теми "Cytochemistry"

Автор: Grafiati

Опубліковано: 4 червня 2021

Оновлено: 4 березня 2023

Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями

Оберіть тип джерела:

Ознайомтеся зі списками актуальних статей, книг, дисертацій, тез та інших наукових джерел на тему "Cytochemistry".

Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.

Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.

Статті в журналах з теми "Cytochemistry":

Takizawa, T., and T. Saito. "Freeze-Fracture Enzyme Cytochemistry: Application of Enzyme Cytochemistry to Freeze-Fracture Cytochemistry." Journal of Electron Microscopy 45, no. 3 (June 1, 1996): 242–46. http://dx.doi.org/10.1093/oxfordjournals.jmicro.a023440.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Cuevas, P., J. A. Gutierrez-Diaz, D. Reimers, M. Dujovny, F. G. Diaz, and J. I. Ausman. "Intramembranous cytochemistry." Neurosurgery 20, no. 2 (February 1987): 243???8. http://dx.doi.org/10.1097/00006123-198702000-00008.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Farhi, Diane C. "Haematological Cytochemistry." American Journal of Clinical Pathology 104, no. 2 (August 1, 1995): 230.2–230. http://dx.doi.org/10.1093/ajcp/104.2.230a.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Kitazawa, Sohei, Teruyuki Ohno, Ryuma Haraguchi, and Riko Kitazawa. "Histochemistry, Cytochemistry and Epigenetics." ACTA HISTOCHEMICA ET CYTOCHEMICA 55, no. 1 (February 26, 2022): 1–7. http://dx.doi.org/10.1267/ahc.21-00095.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Amiry-Moghaddam, Mahmood, and Ole Petter Ottersen. "Immunogold cytochemistry in neuroscience." Nature Neuroscience 16, no. 7 (June 25, 2013): 798–804. http://dx.doi.org/10.1038/nn.3418.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Roels, F., B. De Prest, and G. De Pestel. "Liver and chorion cytochemistry." Journal of Inherited Metabolic Disease 18, S1 (January 1995): 155–71. http://dx.doi.org/10.1007/bf00711437.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Gossrau, R. "Cytochemistry of membrane proteases." Histochemical Journal 17, no. 7 (July 1985): 737–71. http://dx.doi.org/10.1007/bf01003312.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Neame, PB, P. Soamboonsrup, GP Browman, RM Meyer, A. Benger, WE Wilson, IR Walker, N. Saeed, and JA McBride. "Classifying acute leukemia by immunophenotyping: a combined FAB- immunologic classification of AML." Blood 68, no. 6 (December 1, 1986): 1355–62. http://dx.doi.org/10.1182/blood.v68.6.1355.1355.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Abstract A panel of commercially available monoclonal antibodies and five heteroantisera were used to distinguish and subtype 138 cases of acute leukemia (AL). The immunophenotype was compared with the French- American-British (FAB) classification obtained on the cases. The immunophenotype discriminated acute myelogenous leukemia (AML) from acute lymphoblastic leukemia (ALL) and recognized cases not distinguished by cytochemistry (22% of cases), mixed lineage phenotypes (13% of cases), and cases with separate populations of lymphoblasts and myeloblasts (one case). Using the immunologic panel and derived criteria to subtype AML, correspondence of the immunophenotype to the FAB subtypes M1, M2, M4, and M5 was possible in greater than 80% of cases. A combined classification of the immunophenotype and FAB morphology/cytochemistry was devised for AML subtyping. It is recommended that immunophenotyping should be done at least in all cases with negative orinconclusive cytochemistry. At present, we suggest that until a “gold standard” for identifying leukemic subtypes is developed, the best method for typing acute leukemia is by using a combination of morphology, cytochemistry and immunophenotyping.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

A panel of commercially available monoclonal antibodies and five heteroantisera were used to distinguish and subtype 138 cases of acute leukemia (AL). The immunophenotype was compared with the French- American-British (FAB) classification obtained on the cases. The immunophenotype discriminated acute myelogenous leukemia (AML) from acute lymphoblastic leukemia (ALL) and recognized cases not distinguished by cytochemistry (22% of cases), mixed lineage phenotypes (13% of cases), and cases with separate populations of lymphoblasts and myeloblasts (one case). Using the immunologic panel and derived criteria to subtype AML, correspondence of the immunophenotype to the FAB subtypes M1, M2, M4, and M5 was possible in greater than 80% of cases. A combined classification of the immunophenotype and FAB morphology/cytochemistry was devised for AML subtyping. It is recommended that immunophenotyping should be done at least in all cases with negative orinconclusive cytochemistry. At present, we suggest that until a “gold standard” for identifying leukemic subtypes is developed, the best method for typing acute leukemia is by using a combination of morphology, cytochemistry and immunophenotyping.

Takizawa, Toshihiro. "5′-Nucleotidase in Rat Photoreceptor Cells and Pigment Epithelial Cells Processed by Rapid-freezing Enzyme Cytochemistry." Journal of Histochemistry & Cytochemistry 46, no. 9 (September 1998): 1091–95. http://dx.doi.org/10.1177/002215549804600914.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

This report describes the subcellular distribution of 5′-nucleotidase (5′-NT) in rat photoreceptor cells and pigment epithelial cells processed by rapid-freeze enzyme cytochemistry. There was a striking difference in the ultrastructural localization of 5′-NT activity between rod outer segments after freeze-substitution fixation and conventional fixation. By rapid-freezing enzyme cytochemistry, 5′-NT activity was localized in the extradiscal space of intact nonvacuolated discs, whereas by conventional cytochemistry it was shown in the intradiscal space of artifactual vacuolated discs. In the freeze-substituted retinal cells, an appreciable difference in functional 5′-NT molecules was also found. The soluble 5′-NT on the cytoplasmic side of the disc membrane was vital in the rod outer segments, whereas the membrane-bound ecto-5′-NT on the exoplasmic (external) surface of the apical process was active in the pigment epithelial cells. Rapid-freezing enzyme cytochemistry should be worth employing as a method to reveal the fine localization of enzyme activity at the level of cell ultrastructures, which are poorly preserved by conventional fixation, and should provide information approximate to that in living cells.

Більше джерел

Дисертації з теми "Cytochemistry":

Paniccia, Rocco. "Detection of nucleoplasmic glycoconjugates using lectin cytochemistry." Thesis, McGill University, 1990. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=59968.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

The lectins Ulex europaeus 1 (UEA 1), Ricinus communis 1 (RCA 1), Wheat Germ Agglutinin (WGA), and Lens culinaris agglutinin (LCA) which are specific for L-fucose, D-galactose, N-acetylglucosamine, and D-mannose respectively, have been used to localize cytochemically the presence of these sugar residues in certain human, mammalian, and amphibian cell types. Thin sections of Lowicryl K4M-embedded human distal colon, rat duodenum, and frog dorsal root ganglion were incubated with UEA 1-gold, RCA 1-gold, WGA/ovomucoid-gold, or LCA-gold. UEA-1, RCA-1, and LCA binding sites were present in the nuclei of human colonic cells, rat duodenal goblet and columnar cells and frog dorsal root ganglion neurons, satellite cells, and Schwann cells. Most of the nuclear binding sites were localized in the euchromatin compartment, while extranuclear labelling was largely restricted to sites known to contain glycoproteins. WGA binding sites were present in the nuclei of rat duodenum columnar cells and frog dorsal root ganglion neurons, satellite cells, and Schwann cells, but no labelling of human colonic cell nuclei was observed. Thus, we report the presence of these sugars in the nuceloplasm of human colonic cells (with the exception of WGA), rat duodenal villous columnar cells, and frog dorsal root ganglion cells examined.

Henry, Yvonne. "An ultrastructural study of the secretory cycle in digestive glands of Dionaea muscipula Ellis." Thesis, Queen's University Belfast, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.235851.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Long, Joel Andrew. "Ultrastructure and cytochemistry of sporogenesis in two bryophytes, Notothylas and Takakia /." Available to subscribers only, 2006. http://proquest.umi.com/pqdweb?did=1251821251&sid=10&Fmt=2&clientId=1509&RQT=309&VName=PQD.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Song, Xiu-Zhen. "Immunocytochemical localization of photosystems I and II in the green alga Tetraselmis subcordiformis." Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=26152.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

The distribution of photosystem I (PS I) and photosystem II (PS II) in a primitive green alga Tetraselmis subcordiformis, which belongs to Prasinophyceae and does not have grana in its chloroplast, was studied by immunoelectron microscopy. Two PS I antibodies were used: one against a PS I component of maize, the other against the 60 and 62 KDa PS I reaction centre proteins of the cyanobacterium Synechococcus elongatus. Both antibodies showed that 76-78% of the labelling is on the appressed thylakoid membranes and only 22-24% is located on the unappressed membranes. Use of antiserum against cp-47 of PS II from S. elongatus also gives 76% of the labelling on appressed thylakoid membranes and 24% on unappressed thylakoid membranes. Cytochemical detection of PS I activity by the photooxidation of 3,3$ sp prime$-diaminobenzidine and of PS II activity by the photoreduction of distyryl nitroblue tetrazolium chloride also revealed that PS I and PS II activities exist on both types of thylakoid membranes. Therefore, our results indicate that the distribution of PS I and PS II in green algae may differ from that in higher plants.

Wang, Yuchong. "High-throughput investigations of the sub-cellular localisation of proteins and lipids in Saccharomyces cerevisiae." Thesis, University of Cambridge, 2015. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.709145.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Stenzel, Deborah Joan. "Ultrastructural and cytochemical studies of blastocystis sp." Thesis, Queensland University of Technology, 1994.

Знайти повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Ameye, Laurent. "Control of biomineralization in echinoderms :ultrastructure and cytochemistry of the organic matrix." Doctoral thesis, Universite Libre de Bruxelles, 1999. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/211943.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Soole, Kathleen Lydia. "Characterisation of the NADH dehydrogenases associated with isolated plant mitochondria /." Title page, contents and summary only, 1989. http://web4.library.adelaide.edu.au/theses/09PH/09phs711.pdf.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Mutasa, H. C. F. "Cytology, cytochemistry and ultrastructure of blood cells with special reference to myeloid leukaemias." Thesis, University of Cambridge, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335133.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Tang, Liang. "Characterization of tubulins from parasitic nematodes (Brugia malayi, B. pahangi and Nippostrongylus brasiliensis) and comparison with mammalian brain tubulin." Thesis, McGill University, 1988. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=75933.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

The properties of tubulins from Brugia malayi, B. pahangi, Nippostrongylus brasiliensis and rat brain were compared. Tubulins from all nematodes and rat brain were partially purified by polylysine agarose chromatography, those of brain also by cycles of assembly/disassembly, and all by taxol-induced assembly. The tubulins were compared with respect to concentration ($ mu$g tubulin/mg soluble protein), drugs binding and isoforms. The tubulins of B. malayi and B. pahangi were similar. However, the tubulin from these filariae were different from those of N. brasiliensis. Even larger differences were detected between the nematode tubulins and those of rat brain. However, all tubulins reacted to $ alpha$- and $ beta$-tubulin monoclonal antibodies, and had similar mobility on SDS-PAGE. Different peptide maps were obtained for N. brasiliensis tubulin compared with rat brain tubulin. Tubulins of N. brasiliensis bound more mebendazole than did those of Brugia nematodes (B$ sb{ rm max}$: pmoles/$ mu$g tubulin). The binding of benzimidazoles to nematode tubulins was much higher than to rat brain tubulin. Benzimidazole binding to brain tubulin was influenced by the degree of assembly of the tubulin. This did not appear to be the case for the nematode tubulins. In vitro translation of B. malayi mRNA resulted in two isoforms for both $ alpha$- and $ beta$-tubulins in contrast to the 4 $ alpha$- and 4-5 $ beta$-isoforms found naturally. This suggest post translational modification of tubulin may take place in B. malayi. This study has characterized some of the differences that exist between mammalian tubulins and those of nematodes on the one hand, and between the tubulins of a gastrointestinal nematode (N. brasiliensis) and those of filariae (B. malayi and B. pahangi) on the other hand.

Більше джерел

Книги з теми "Cytochemistry":

Hayhoe, F. G. J. Haematological cytochemistry. 3rd ed. Edinburgh: Churchill Livingstone, 1994.

Знайти повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

C, Vaughn Kevin, ed. CRC handbook of plant cytochemistry. Boca Raton, Fla: CRC Press, 1987.

Знайти повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Yamashita, Shuji. Histochemistry and cytochemistry of nuclear receptors. Jena, Germany: Urban & Fischer, 2001.

Знайти повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Dashek, William V. Methods in Plant Electron Microscopy and Cytochemistry. New Jersey: Humana Press, 2000. http://dx.doi.org/10.1385/1592592325.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Dashek, William V., ed. Methods in Plant Electron Microscopy and Cytochemistry. Totowa, NJ: Humana Press, 2000. http://dx.doi.org/10.1007/978-1-59259-232-6.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Sun, Tsieh. Atlas of cytochemistry & immunochemistry of hematologic neoplasms. Chicago: American Society of Clinical Pathologists Press, 1985.

Знайти повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Vorbrodt, Andrzej. Ultrastructural cytochemistry of blood-brain barrier endothelia. Stuttgart: G. Fischer, 1988.

Знайти повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

1928-, Ogawa Kazuo, and Barka Tibor, eds. Electron microscopic cytochemistry and immunocytochemistry in biomedicine. Boca Raton: CRC Press, 1993.

Знайти повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

V, Dashek William, ed. Methods in plant electron microscopy and cytochemistry. Totowa, N.J: Humana Press, 2000.

Знайти повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Yoshihiro, Akimoto, ed. Histochemistry and cytochemistry of endogenous animal lectins. Jena: Gustav Fischer, 1998.

Знайти повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Більше джерел

Частини книг з теми "Cytochemistry":

Pavelka, Margit. "Cytochemistry." In Functional Morphology of the Golgi Apparatus, 33–55. Berlin, Heidelberg: Springer Berlin Heidelberg, 1987. http://dx.doi.org/10.1007/978-3-642-72826-6_4.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Dykstra, Michael J., and Laura E. Reuss. "Cytochemistry." In Biological Electron Microscopy, 197–208. Boston, MA: Springer US, 2003. http://dx.doi.org/10.1007/978-1-4419-9244-4_9.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Dykstra, Michael J. "Cytochemistry." In Biological Electron Microscopy, 295–308. Boston, MA: Springer US, 1992. http://dx.doi.org/10.1007/978-1-4684-0010-6_14.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Patel, Kamlesh R., and Trevor A. Thorpe. "Morphogenesis (Cytochemistry)." In Cell and Tissue Culture in Forestry, 183–201. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-4484-8_9.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Miquel, Jaime. "Ultrastructure and cytochemistry." In Drosophila as a Model Organism for Ageing Studies, 193–200. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4899-2683-8_15.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Petrali, John P., and Kenneth R. Mills. "Microwave-Assisted Cytochemistry." In Springer Protocols Handbooks, 165–71. Totowa, NJ: Humana Press, 2001. http://dx.doi.org/10.1007/978-1-59259-128-2_13.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Morré, D. J. "Plasma Membrane Cytochemistry." In The Plant Plasma Membrane, 76–92. Berlin, Heidelberg: Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-74522-5_4.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

van Deurs., B., M. Møller, and O. W. Petersen. "Ultrastructural Cytochemistry and Immunocytochemistry." In Theory and Strategy in Histochemistry, 387–95. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-73742-8_27.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Roels, F., B. De Prest, and G. De Pestel. "Liver and chorion cytochemistry." In Diagnosis of human peroxisomal disorders, 155–71. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-011-9635-2_13.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Van Duijn, P., and M. Van Der Ploeg. "Microscopic Cytochemistry as Matrix Chemistry." In Ciba Foundation Symposium 73 - Trends in Enzyme Histochemistry and Cytochemistry, 209–29. Chichester, UK: John Wiley & Sons, Ltd., 2008. http://dx.doi.org/10.1002/9780470720561.ch12.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Тези доповідей конференцій з теми "Cytochemistry":

Demircan, Turan. "Differential Proteomics Analysis of Axolotl’s Regenerating Tail." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.op-08.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Yucel, Deniz. "The Behavior of Menstrual Blood-Derived Stem Cells on Electrospun Polymeric Fibers." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.op-09.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Tuğlu, Ibrahim Mehmet. "The effect of Electro Magnetic Fields on Stem and Cancer Cell." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.op-10.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Bhouri, Wissem. "Biological activities of natural compounds extracted from {rhamnus alaternus} plant." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.op-11.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Bilgic, Elif. "Endocannabinoid induced apoptotic cell death on endometriotic cells." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.op-12.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Yildiz, Mustafa. "Effect of folic acid on testicular toxicity induced by bisphenol A in male Wistar rats." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.op-13.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Cumbul, Alev. "Optimizing Hydroxy Apatite Based Scaffold with Harmony of Stem Cells for Tooth Tissue Engineering." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.op-42.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Kostal, Vratislav. "Label-free investigation of cancer cell behavior using quantitative phase imaging." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.op-58.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Karaca, Turan. "Pendrin and sodium / iodide symporter (NIS) protein expression in testicular tissue of diabetic rat*." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.pp-167.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Beyaz, Feyzullah. "Immunohistochemical investigation of TLR4 in rat testis and epididymis during postnatal development." In 15th International Congress of Histochemistry and Cytochemistry. Istanbul: LookUs Scientific, 2017. http://dx.doi.org/10.5505/2017ichc.pp-195.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Звіти організацій з теми "Cytochemistry":

Zlotkin, Eliahu, Shizuo G. Kamita, Nor Chejanovsky, and S. Maeda. Targeting of an Expressed Insect Selective Neurotoxin by its Recombinant Baculovirus: Pharmacokinetic and Pharmacodynamic Aspects. United States Department of Agriculture, July 1995. http://dx.doi.org/10.32747/1995.7571354.bard.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Анотація:

Objectives: 1) Clarification of the mode of potentiation of an expressed insect selective neurotoxin (AaIT) by its recombinant baculovirus. 2) In vitro formation and/or modification of neuroactive polypeptides for the design of new improved recombinant baculoviruses. Results: 1) A combined utilization of bioassays, LM-cytochemistry, the highly resolutive EM immunogold and electrical recording from the CNS of baculovirus and AaIT - expressing – recombinant baculovirus infected larvae it has been shown that the recombinant virus potentiates the effect of the toxin. Potentiation is achieved through its continuous expression in the infected tracheal epithelia thus providing a: a) Local supply of freshly produced toxin in the vicinity of its traget sites; b) Translocation of the expressed toxin to the insect CNS. The latter exposes the recombinant toxin to new, critical, target sites which are inaccessible through the natural route of scorpion envenomation. 2) Subjecting a recombinant AaIT toxin to a newly designed system of random mutagenesis results in large numbers of new AaIT genes with amino acid substitutions. The new or modified toxin genes were inserted into a linear BmNPV expressed in silkworm cell culture and assayed on blowfly and silkworm larvae. Thus a system for mass formation and screening of neuroactive agents was developed. Contribution to agriculture: 1) Demonstration of the insecticidal mechanism, capacity and utility of the combination of neuroactive polypeptides and recombinant pathogens. 2) Development of a simple in vitro system for the formation and selection of new neuroactive polypeptides.