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Добірка наукової літератури з теми "Cyanobacteria"

Автор: Grafiati

Опубліковано: 4 червня 2021

Оновлено: 30 липня 2024

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Статті в журналах з теми "Cyanobacteria"

Nakayama, Takuro, Mami Nomura, Yoshihito Takano, Goro Tanifuji, Kogiku Shiba, Kazuo Inaba, Yuji Inagaki, and Masakado Kawata. "Single-cell genomics unveiled a cryptic cyanobacterial lineage with a worldwide distribution hidden by a dinoflagellate host." Proceedings of the National Academy of Sciences 116, no. 32 (June 24, 2019): 15973–78. http://dx.doi.org/10.1073/pnas.1902538116.

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Cyanobacteria are one of the most important contributors to oceanic primary production and survive in a wide range of marine habitats. Much effort has been made to understand their ecological features, diversity, and evolution, based mainly on data from free-living cyanobacterial species. In addition, symbiosis has emerged as an important lifestyle of oceanic microbes and increasing knowledge of cyanobacteria in symbiotic relationships with unicellular eukaryotes suggests their significance in understanding the global oceanic ecosystem. However, detailed characteristics of these cyanobacteria remain poorly described. To gain better insight into marine cyanobacteria in symbiosis, we sequenced the genome of cyanobacteria collected from a cell of a pelagic dinoflagellate that is known to host cyanobacterial symbionts within a specialized chamber. Phylogenetic analyses using the genome sequence revealed that the cyanobacterium represents an underdescribed lineage within an extensively studied, ecologically important group of marine cyanobacteria. Metagenomic analyses demonstrated that this cyanobacterial lineage is globally distributed and strictly coexists with its host dinoflagellates, suggesting that the intimate symbiotic association allowed the cyanobacteria to escape from previous metagenomic studies. Furthermore, a comparative analysis of the protein repertoire with related species indicated that the lineage has independently undergone reductive genome evolution to a similar extent as Prochlorococcus, which has the most reduced genomes among free-living cyanobacteria. Discovery of this cyanobacterial lineage, hidden by its symbiotic lifestyle, provides crucial insights into the diversity, ecology, and evolution of marine cyanobacteria and suggests the existence of other undiscovered cryptic cyanobacterial lineages.

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Hurley, Sarah J., Boswell A. Wing, Claire E. Jasper, Nicholas C. Hill, and Jeffrey C. Cameron. "Carbon isotope evidence for the global physiology of Proterozoic cyanobacteria." Science Advances 7, no. 2 (January 2021): eabc8998. http://dx.doi.org/10.1126/sciadv.abc8998.

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Ancestral cyanobacteria are assumed to be prominent primary producers after the Great Oxidation Event [≈2.4 to 2.0 billion years (Ga) ago], but carbon isotope fractionation by extant marine cyanobacteria (α-cyanobacteria) is inconsistent with isotopic records of carbon fixation by primary producers in the mid-Proterozoic eon (1.8 to 1.0 Ga ago). To resolve this disagreement, we quantified carbon isotope fractionation by a wild-type planktic β-cyanobacterium (Synechococcus sp. PCC 7002), an engineered Proterozoic analog lacking a CO2-concentrating mechanism, and cyanobacterial mats. At mid-Proterozoic pH and pCO2 values, carbon isotope fractionation by the wild-type β-cyanobacterium is fully consistent with the Proterozoic carbon isotope record, suggesting that cyanobacteria with CO2-concentrating mechanisms were apparently the major primary producers in the pelagic Proterozoic ocean, despite atmospheric CO2 levels up to 100 times modern. The selectively permeable microcompartments central to cyanobacterial CO2-concentrating mechanisms (“carboxysomes”) likely emerged to shield rubisco from O2 during the Great Oxidation Event.

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Kollmen, Jonas, and Dorina Strieth. "The Beneficial Effects of Cyanobacterial Co-Culture on Plant Growth." Life 12, no. 2 (January 31, 2022): 223. http://dx.doi.org/10.3390/life12020223.

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Cyanobacteria are ubiquitous phototrophic prokaryotes that find a wide range of applications in industry due to their broad product spectrum. In this context, the application of cyanobacteria as biofertilizers and thus as an alternative to artificial fertilizers has emerged in recent decades. The benefit is mostly based on the ability of cyanobacteria to fix elemental nitrogen and make it available to the plants in a usable form. However, the positive effects of co-cultivating plants with cyanobacteria are not limited to the provision of nitrogen. Cyanobacteria produce numerous secondary metabolites that can be useful for plants, for example, they can have growth-promoting effects or increase resistance to plant diseases. The effects of biotic and abiotic stress can as well be reduced by many secondary metabolites. Furthermore, the biofilms formed by the cyanobacteria can lead to improved soil conditions, such as increased water retention capacity. To exchange the substances mentioned, cyanobacteria form symbioses with plants, whereby the strength of the symbiosis depends on both partners, and not every plant can form symbiosis with every cyanobacterium. Not only the plants in symbiosis benefit from the cyanobacteria, but also vice versa. This review summarizes the beneficial effects of cyanobacterial co-cultivation on plants, highlighting the substances exchanged and the strength of cyanobacterial symbioses with plants. A detailed explanation of the mechanism of nitrogen fixation in cyanobacterial heterocysts is given. Finally, a summary of possible applications of co-cultivation in the (agrar-)industry is given.

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Rangel, Luciana M., Lúcia H. S. Silva, Elisabeth J. Faassen, Miquel Lürling, and Kemal Ali Ger. "Copepod Prey Selection and Grazing Efficiency Mediated by Chemical and Morphological Defensive Traits of Cyanobacteria." Toxins 12, no. 7 (July 21, 2020): 465. http://dx.doi.org/10.3390/toxins12070465.

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Phytoplankton anti-grazer traits control zooplankton grazing and are associated with harmful blooms. Yet, how morphological versus chemical phytoplankton defenses regulate zooplankton grazing is poorly understood. We compared zooplankton grazing and prey selection by contrasting morphological (filament length: short vs. long) and chemical (saxitoxin: STX- vs. STX+) traits of a bloom-forming cyanobacterium (Raphidiopsis) offered at different concentrations in mixed diets with an edible phytoplankton to a copepod grazer. The copepod selectively grazed on the edible prey (avoidance of cyanobacteria) even when the cyanobacterium was dominant. Avoidance of the cyanobacterium was weakest for the “short STX-” filaments and strongest for the other three strains. Hence, filament size had an effect on cyanobacterial avoidance only in the STX- treatments, while toxin production significantly increased cyanobacterial avoidance regardless of filament size. Moreover, cyanobacterial dominance reduced grazing on the edible prey by almost 50%. Results emphasize that the dominance of filamentous cyanobacteria such as Raphidiopsis can interfere with copepod grazing in a trait specific manner. For cyanobacteria, toxin production may be more effective than filament size as an anti-grazer defense against selectively grazing zooplankton such as copepods. Our results highlight how multiple phytoplankton defensive traits interact to regulate the producer-consumer link in plankton ecosystems.

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Rajabpour, Nooshin, Bahareh Nowruzi, and Maryam Ghobeh. "Investigation of the toxicity, antioxidant and antimicrobial activities of some cyanobacterial strains isolated from different habitats." Acta Biologica Slovenica 62, no. 2 (December 1, 2019): 4–12. http://dx.doi.org/10.14720/abs.62.2.15753.

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Cyanobacteria are known as a source of fine chemicals, renewable fuels, and toxic compounds. The present study aimed at evaluating the toxicity and antioxidant and antimicrobial activities of four cyanobacterial strains isolated from different habitats. Due to the lack of information regarding the relationship between toxicity and biological activity of the cyanobacteria in terrestrial and aquatic ecosystems of Iran, we decided to conduct a preliminary study on the cyanobacterial strains in order to identify the potentially toxic cyanobacteria strains. In this respect, biosynthesis genes related to cyanobacterial toxins, anatoxins (anaC gene), nodularins (ndaF gene) and microcystins (mcyG gene) were amplified. In addition, antioxidant, antimicrobial and biochemical properties of cyanobacterial strains have also been evaluated. The results of the molecular analysis demonstrated that only Fischerella sp. contained the microcystins (mcyG) gene. In fact, this strain encounters numerous predators in its habitat, therefore antibacterial and antioxidant metabolites found in this strain have thought to play an important role in defense mechanisms. This case is the documentation of toxicity and promotion of biological activities of a soil cyanobacterium regarding survival in competitive ecological niches.

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Foster, Rachel A., and Jonathan P. Zehr. "Diversity, Genomics, and Distribution of Phytoplankton-Cyanobacterium Single-Cell Symbiotic Associations." Annual Review of Microbiology 73, no. 1 (September 8, 2019): 435–56. http://dx.doi.org/10.1146/annurev-micro-090817-062650.

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Cyanobacteria are common in symbiotic relationships with diverse multicellular organisms (animals, plants, fungi) in terrestrial environments and with single-celled heterotrophic, mixotrophic, and autotrophic protists in aquatic environments. In the sunlit zones of aquatic environments, diverse cyanobacterial symbioses exist with autotrophic taxa in phytoplankton, including dinoflagellates, diatoms, and haptophytes (prymnesiophytes). Phototrophic unicellular cyanobacteria related to Synechococcus and Prochlorococcus are associated with a number of groups. N2-fixing cyanobacteria are symbiotic with diatoms and haptophytes. Extensive genome reduction is involved in the N2-fixing endosymbionts, most dramatically in the unicellular cyanobacteria associated with haptophytes, which have lost most of the photosynthetic apparatus, the ability to fix C, and the tricarboxylic acid cycle. The mechanisms involved in N2-fixing symbioses may involve more interactions beyond simple exchange of fixed C for N. N2-fixing cyanobacterial symbioses are widespread in the oceans, even more widely distributed than the best-known free-living N2-fixing cyanobacteria, suggesting they may be equally or more important in the global ocean biogeochemical cycle of N.Despite their ubiquitous nature and significance in biogeochemical cycles, cyanobacterium-phytoplankton symbioses remain understudied and poorly understood.

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Caraco, N. F., and R. Miller. "Effects of CO2 on competition between a cyanobacterium and eukaryotic phytoplankton." Canadian Journal of Fisheries and Aquatic Sciences 55, no. 1 (January 1, 1998): 54–62. http://dx.doi.org/10.1139/f97-202.

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To distinguish whether there is a causal link between cyanobacterial dominance and low CO2 and (or) the associated high pH, we ran duplicate competition experiments using a factorial design of CO2 by alkalinity. In various treatments, three concentrations of alkalinity (ca. 50, 500, and 5000 µequiv. ·L-1) and CO2 (ca. 1.3, 13, and 130 µM) generated three pH values (ca. 7, 8, and 9). At the end of about a 1-week incubation, Aphanizomenon flos aquae was the only cyanobacterium present, while the chlorophytes Scenedesmus and Selenastrum along with unidentified flagellates comprised the eukaryotic phytoplankton. The treatments had a dramatic effect on cyanobacterial biomass, which varied from >90% to 0% of the total phytoplankton biomass across treatments. Variation in percent cyanobacteria was better related to pH than to CO2. At pH 8 and 9, percent cyanobacteria was relatively high at all CO2 concentrations. Only at pH 7 was percent cyanobacteria negatively related to CO2 concentration. These results demonstrate both direct and indirect effects of CO2 on cyanobacterial dominance but suggest that, for A. flos aquae, the indirect impact of CO2 (pH alteration) is most important. The impact of CO2 on this cyanobacterium, therefore, depends on the alkalinity of the system.

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Deng, Ming-De, and John R. Coleman. "Ethanol Synthesis by Genetic Engineering in Cyanobacteria." Applied and Environmental Microbiology 65, no. 2 (February 1, 1999): 523–28. http://dx.doi.org/10.1128/aem.65.2.523-528.1999.

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ABSTRACT Cyanobacteria are autotrophic prokaryotes which carry out oxygenic photosynthesis and accumulate glycogen as the major form of stored carbon. In this research, we introduced new genes into a cyanobacterium in order to create a novel pathway for fixed carbon utilization which results in the synthesis of ethanol. The coding sequences of pyruvate decarboxylase (pdc) and alcohol dehydrogenase II (adh) from the bacterium Zymomonas mobilis were cloned into the shuttle vector pCB4 and then used to transform the cyanobacterium Synechococcus sp. strain PCC 7942. Under control of the promoter from the rbcLS operon encoding the cyanobacterial ribulose-1,5-bisphosphate carboxylase/oxygenase, thepdc and adh genes were expressed at high levels, as demonstrated by Western blotting and enzyme activity analyses. The transformed cyanobacterium synthesized ethanol, which diffused from the cells into the culture medium. As cyanobacteria have simple growth requirements and use light, CO2, and inorganic elements efficiently, production of ethanol by cyanobacteria is a potential system for bioconversion of solar energy and CO2 into a valuable resource.

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Olsson-Francis, Karen, Rosa de la Torre, and Charles S. Cockell. "Isolation of Novel Extreme-Tolerant Cyanobacteria from a Rock-Dwelling Microbial Community by Using Exposure to Low Earth Orbit." Applied and Environmental Microbiology 76, no. 7 (February 12, 2010): 2115–21. http://dx.doi.org/10.1128/aem.02547-09.

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ABSTRACT Many cyanobacteria are known to tolerate environmental extremes. Motivated by an interest in selecting cyanobacteria for applications in space, we launched rocks from a limestone cliff in Beer, Devon, United Kingdom, containing an epilithic and endolithic rock-dwelling community of cyanobacteria into low Earth orbit (LEO) at a height of approximately 300 kilometers. The community was exposed for 10 days to isolate cyanobacteria that can survive exposure to the extreme radiation and desiccating conditions associated with space. Culture-independent (16S rRNA) and culture-dependent methods showed that the cyanobacterial community was composed of Pleurocapsales, Oscillatoriales, and Chroococcales. A single cyanobacterium, a previously uncharacterized extremophile, was isolated after exposure to LEO. We were able to isolate the cyanobacterium from the limestone cliff after exposing the rock-dwelling community to desiccation and vacuum (0.7 Ã¯Â¿Â½ 10−3 kPa) in the laboratory. The ability of the organism to survive the conditions in space may be linked to the formation of dense colonies. These experiments show how extreme environmental conditions, including space, can be used to select for novel microorganisms. Furthermore, it improves our knowledge of environmental tolerances of extremophilic rock-dwelling cyanobacteria.

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Dash, Sidhartha Kumar, Jitendra Kumar Pandey, Mrutyunjay Jena, and Basanti Biswal. "Effect of Heat Stress and the Recovery Potential of Heterocystous Cyanobacterium, Anabaena iyengarii Bharadwaja 1935." Journal of Pure and Applied Microbiology 14, no. 4 (December 16, 2020): 2467–76. http://dx.doi.org/10.22207/jpam.14.4.24.

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Cyanobacteria, the major photosynthetic organisms, cover a large surface area of this planet. These organisms, being photosynthetic, have the capacity for sequestration of atmospheric carbon dioxide, a significant greenhouse gas that causes global warming. In this work, we have collected, developed pure culture, and identified 25 cyanobacterial species from semi arid agricultural rice fields of western Odisha with the high-temperature environmental setting. The purpose was to screen the cyanobacteria that can survive and grow at high temperatures with high photosynthetic efficiency. Cyanobacteria belong to genera Nostoc, Anabaena, Calothrix, and Hapalosiphon are observed to survive at 45°C. Among the cyanobacterial species, Anabaena iyengarii 17-SKD-2014 was found to exhibit higher growth, protein content, photosynthetic pigments, and photosynthetic O2 evolution at 45°C in comparison to other cyanobacterial isolates. Further, this cyanobacterium was grown at 50°C to analyze the cellular viability, and only up to ninth day incubated culture could recover from high-temperature stress after transferring to 25°C. Even though this indigenous cyanobacterial species failed to survive at 50°C in the laboratory conditions beyond a time limit, but this could be biotechnologically manipulated for effective carbon dioxide sequestration contributing to minimization of global warming.

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Дисертації з теми "Cyanobacteria"

Du, Plooy Schalk Jacobus. "Ecophysiology and nutrient uptake mechanisms facilitating the prolonged bloom persistence by Cyanothece sp. in Lake St Lucia, South Africa." Thesis, Nelson Mandela Metropolitan University, 2017. http://hdl.handle.net/10948/7344.

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Cyanobacterial blooms are becoming more frequent worldwide, with possible negative effects on human health. The effects of climate change and eutrophication have been associated with persistent cyanobacterial blooms becoming more frequent. Altered water characteristics, salinity in particular, influence ecosystem dynamics that may lead to conditions conducive to cyanobacterial blooms. The occurrence of an 18-month long Cyanothece sp. bloom (the longest for any cyanobacterium recorded so far worldwide and the first of the genus) from June 2009 to December 2010 in Africa’s largest estuarine lake, St Lucia, highlighted the susceptibility of ecosystems to anthropogenic alterations. This study investigated the long-term survival and physiological adaptations of Cyanothece sp. to various and dynamic environmental conditions that contributed towards its bloom persistence. The main findings are the high salinities at which Cyanothece sp. could perform important physiological processes such as N uptake, N2 fixation and photosynthesis. Nutrient uptake (both nitrogen and phosphorus) was observed over the full experimental salinity range (0-300) while N2 fixation was only observed up to a salinity of 120. Nutrient uptake rates significantly decreased at this threshold salinity of 120. Interestingly, photosystem II activity was not observed in Cyanothece sp. during this study, but photosystem I activity was robust. Salinity had a minor influence on electron transport rates by photosystem I, high temperature (> 30°C) did however increase electron transport rates. Rapid responses to hypo-osmotic shock (i.e. osmotic downshift during freshening events) by Cyanothece sp. cells also helped minimize cell rupture due to high turgor pressure. Zooplankton abundance within the St Lucia system was negatively correlated with salinity, while grazing experiments indicated that the typical estuarine zooplankton species are able to graze on Cyanothece sp. cells. Therefore, the disappearance of zooplankton at salinities above 60 must have been an important factor in the bloom persistence. Apart from the ecological factors that were at play in St Lucia during the bloom period, the persistence of the Cyanothece sp. bloom can be attributed to the robust nature of their nutrient uptake, nitrogen fixation and photosynthetic systems to maintain activity despite extreme hypersalinity levels.

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Froscio, Suzanne M. "Investigation of the mechanisms involved in cylindrospermopsin toxicity : hepatocyte culture and reticulocyte lysate studies." Title page, contents and abstract only, 2002. http://web4.library.adelaide.edu.au/theses/09PH/09phf938.pdf.

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Bibliography: leaves 121-139. The aim of this study was to determine the extent to which protein synthesis inhibition, lowered glutathione (GSH) levels and toxin metabolism contribute to the toxicity of cyclindrospermopsin. Both hepatocyte cultures and reticulocyte lysates were utilized as in vitro tools of investigation. The findings imply that the inhibition of protein synthesis by direct action of the toxin cannot be considered a primary cause of hepatocyte cell death over an acute time frame. Cytochrome P450-derived metabolites may play a crucial role in cytotoxicity, and the toxicity process does not appear to involve oxidative damage.

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Stewart, Ian. "Recreational exposure to freshwater cyanobacteria : epidemiology, dermal toxicity and biological activity of cyanobacterial lipopolysaccharides /." [St. Lucia, Qld.], 2004. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe.pdf.

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Wang, Kai. "INTERACTIONS OF CYANOBACTERIA AND CO-OCCURRING MICROORGANISMS DURING CYANOBACTERIAL HARMFUL ALGAL BLOOMS." Kent State University / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=kent1619622253977384.

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Menke, Sharon M. "NifD: Its Evolution and Phylogenetic Use in Cyanobacteria." Miami University / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=miami1176983927.

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Lindberg, Pia. "Cyanobacterial Hydrogen Metabolism - Uptake Hydrogenase and Hydrogen Production by Nitrogenase in Filamentous Cyanobacteria." Doctoral thesis, Uppsala University, Physiological Botany, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3541.

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Molecular hydrogen is a potential energy carrier for the future. Nitrogen-fixing cyanobacteria are a group of photosynthetic microorganisms with the inherent ability to produce molecular hydrogen via the enzyme complex nitrogenase. This hydrogen is not released, however, but is recaptured by the bacteria using an uptake hydrogenase. In this thesis, genes involved in cyanobacterial hydrogen metabolism were examined, and the possibility of employing genetically modified cyanobacteria for hydrogen production was investigated.

Nostoc punctiforme PCC 73102 (ATCC 29133) is a nitrogen-fixing filamentous cyanobacterium containing an uptake hydrogenase encoded by hupSL. The transcription of hupSL was characterised, and putative regulatory elements in the region upstream of the transcription start site were identified. One of these, a binding motif for the global nitrogen regulator NtcA, was further investigated by mobility shift assays, and it was found that the motif is functional in binding NtcA. Also, a set of genes involved in maturation of hydrogenases was identified in N. punctiforme, the hypFCDEAB operon. These genes were found to be situated upstream of hupSL in the opposite direction, and they were preceded by a previously unknown open reading frame, that was found to be transcribed as part of the same operon.

The potential for hydrogen production by filamentous cyanobacteria was investigated by studying mutant strains lacking an uptake hydrogenase. A mutant strain of N. punctiforme was constructed, where hupL was inactivated. It was found that cultures of this strain evolve hydrogen during nitrogen fixation. Gas exchange in the hupL^- mutant and in wild type N. punctiforme was measured using a mass spectrometer, and conditions under which hydrogen production from the nitrogenase could be increased at the expense of nitrogen fixation were identified. Growth and hydrogen production in continuous cultures of a Hup^- mutant of the related strain Nostoc PCC 7120 were also studied.

This thesis advances the knowledge about cyanobacterial hydrogen metabolism and opens possibilities for further development of a process for hydrogen production using filamentous cyanobacteria.

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Berry, Gerald A. "Mosquito Larvicides from Cyanobacteria." FIU Digital Commons, 2014. http://digitalcommons.fiu.edu/etd/1449.

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Cyanobacteria (blue-green algae) produce a diverse array of toxic or otherwise bioactive metabolites. These allelochemicals may also play a role in defense against potential predators and grazers, particularly aquatic invertebrates and their larvae, including mosquitoes. Compounds derived from cyanobacteria collected from the Florida Everglades and other Florida waterways were investigated as insecticides against the mosquito Aedes aegypti, a vector of dengue and yellow fever. Screening of cyanobacterial biomass revealed several strains that exhibited mosquito larvicidal activity. Guided via bioassay guided fractionation, a non-polar compound from Leptolyngbya sp. 21-9-3 was found to be the most active component. Characterization revealed the prospective compound to be a monounsaturated fatty acid with the molecular formula C16H30O2. This is the first evidence of mosquito larvicidal activity for this particular fatty acid. With larvicidal becoming more prevalent, fatty acids should be explored for future mosquito control strategies.

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Bibby, T. S. "Photosynthetic complexes of cyanobacteria." Thesis, Imperial College London, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.595520.

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Lee, Elvina. "Molecular systematics of cyanobacteria." Thesis, Lee, Elvina (2016) Molecular systematics of cyanobacteria. PhD thesis, Murdoch University, 2016. https://researchrepository.murdoch.edu.au/id/eprint/34883/.

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Cyanobacteria constitute a phylum of ubiquitous cosmopolitan bacteria with the ability to perform oxygenic photosynthesis. Their ancient origins, ecological and economic potential, biotechnological applications and impact on water systems have attracted much interest from the academia, industry, health authorities and regulators. Despite this, cyanobacteria classification and nomenclature still remains difficult. One of the aims of this project was to further our understanding of cyanobacteria systematics by (1) testing polyphasic characterization methods and (2) examining the effect of various phylogenetic reconstruction strategies. Additionally, (3) Next Generation Sequencing (NGS) assays using novel cyanobacteria 16S rDNA targeted primers were implemented to provide better taxa resolution than that offered by “universal” 16S rDNA primers. Cyanobacteria strains from various water sources in Australia were isolated, characterised at four loci commonly used for cyanobacteria molecular classification, and tested for the presence of genes implicated in toxin and terpene (odour) production. A total of 55 novel cyanobacterial strains were isolated and maintained in culture, forming the first known collection of cyanobacteria isolates from Western Australia. Comparison of molecular– and morphology– based identifications not only showed the limitations of the current methods (only 45% of the isolates showed agreement) but also provided the opportunity to suggest guidelines and conceive a way forward towards more effective identification approaches. Examination of alternative phylogenetic markers, workflows and stringencies showed that between alignment algorithms, alignment curations and tree building methods, the latter had the greatest effect on tree topology. This result was consistent regardless of locus, alignment and curation strategy employed. Finally, two sets of novel cyanobacteria-targeted primers were designed for use with NGS technologies. As compared to the universal 16S rRNA primers, these primers showed higher specificity and preferential amplification of cyanobacteria and proteobacteria DNA. Of the sequences obtained using these two new primer pairs, cyanobacteria sequences comprised 50.5% and 54.4%, while proteobacteria sequences comprised 44.5% and 40.3% respectively. In comparison, with the universal 16S rRNA primers, cyanobacteria and proteobacteria comprised 15.3% and 33.4% respectively of the sequences analysed. Using morphological and molecular methods, this project provides a snapshot of the as yet unstudied freshwater cyanobacterial diversity found in Western Australia using polyphasic methods. The limitations of the current identification approaches, uncovered during the first phase of the project, were harnessed to develop a method to assess the variability of phylogenetic reconstructions. Finally, novel cyanobacteria specific NGS primers demonstrated how adopting the latest NGS technology represents a promising advance in the molecular investigation of cyanobacteria.

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Williams, Philip. "Chemical investigations of marine cyanobacteria : the search for new anticancer agents from the sea /." Thesis, University of Hawaii at Manoa, 2003. http://hdl.handle.net/10125/6878.

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Книги з теми "Cyanobacteria"

1968-, Huisman Jef, Matthijs Hans C. P, and Visser Petra M, eds. Harmful cyanobacteria. Dordrecht: Springer, 2005.

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Sharma, Naveen K., Ashwani K. Rai, and Lucas J. Stal, eds. Cyanobacteria. Chichester, UK: John Wiley & Sons, Ltd, 2014. http://dx.doi.org/10.1002/9781118402238.

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Sedmak, Bojan. Cyanobacteria and their toxins: What are they, where can we find them, why are they able to prevail and how do they behave? Ljubljana: National Institute of Biology, 2012.

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Bruno, Milena. Nuove sostanze neurotossiche prodotte da alghe: La [beta]-N-metilammino-L-alanina. Roma: Istituto superiore di sanità, 2012.

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1955-, Rai Amar N., Bergman Birgitta, and Rasmussen Ulla, eds. Cyanobacteria in symbiosis. Dordrecht: Kluwer Academic Pub., 2002.

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Huisman, Jef, Hans C. P. Matthijs, and Petra M. Visser, eds. Harmful Cyanobacteria. Berlin/Heidelberg: Springer-Verlag, 2005. http://dx.doi.org/10.1007/1-4020-3022-3.

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Loïc, Charpy, Larkum A. W. D, and Musée océanographique de Monaco, eds. Marine cyanobacteria. Monaco: Musée océanographique, 1999.

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Peter, Fay, and Van Baalen C. 1925-1986, eds. The Cyanobacteria. Amsterdam, The Netherlands: Elsevier Science Publishers B.V., 1987.

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Kondratʹeva, Nadezhda Vasilʹevna. Morfologii͡a︡ populi͡a︡t͡s︡iĭ prokarioticheskikh vodorosleĭ. Kiev: Nauk. dumka, 1989.

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Rai, Amar N., Birgitta Bergman, and Ulla Rasmussen, eds. Cyanobacteria in Symbiosis. Dordrecht: Springer Netherlands, 2002. http://dx.doi.org/10.1007/0-306-48005-0.

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Частини книг з теми "Cyanobacteria"

Borowitzka, Michael A. "Patents on cyanobacteria and cyanobacterial products and uses." In Cyanobacteria, 329–38. Chichester, UK: John Wiley & Sons, Ltd, 2013. http://dx.doi.org/10.1002/9781118402238.ch21.

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Oren, Aharon. "Cyanobacteria: biology, ecology and evolution." In Cyanobacteria, 1–20. Chichester, UK: John Wiley & Sons, Ltd, 2013. http://dx.doi.org/10.1002/9781118402238.ch1.

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Sharma, Naveen K., and Lucas J. Stal. "The economics of cyanobacteria-based biofuel production: challenges and opportunities." In Cyanobacteria, 167–80. Chichester, UK: John Wiley & Sons, Ltd, 2013. http://dx.doi.org/10.1002/9781118402238.ch10.

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Milou Schuurmans, R., Hans C. P. Matthijs, Lucas J. Stal, and Klaas J. Hellingwerf. "Cyanobacterial cellulose synthesis in the light of the photanol concept." In Cyanobacteria, 181–95. Chichester, UK: John Wiley & Sons, Ltd, 2013. http://dx.doi.org/10.1002/9781118402238.ch11.

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Colica, Giovanni, and Roberto De Philippis. "Exopolysaccharides from cyanobacteria and their possible industrial applications." In Cyanobacteria, 197–207. Chichester, UK: John Wiley & Sons, Ltd, 2013. http://dx.doi.org/10.1002/9781118402238.ch12.

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Bermejo, Ruperto. "Phycocyanins." In Cyanobacteria, 209–25. Chichester, UK: John Wiley & Sons, Ltd, 2013. http://dx.doi.org/10.1002/9781118402238.ch13.

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Samantaray, Shilalipi, Ranjana Bhati, and Nirupama Mallick. "Cyanobacterial polyhydroxyalkanoates: an alternative source for plastics." In Cyanobacteria, 227–44. Chichester, UK: John Wiley & Sons, Ltd, 2013. http://dx.doi.org/10.1002/9781118402238.ch14.

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Hamilton, David P., Susanna A. Wood, Daniel R. Dietrich, and Jonathan Puddick. "Costs of harmful blooms of freshwater cyanobacteria." In Cyanobacteria, 245–56. Chichester, UK: John Wiley & Sons, Ltd, 2013. http://dx.doi.org/10.1002/9781118402238.ch15.

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Woodhouse, Jason N., Melissa Rapadas, and Brett A. Neilan. "Cyanotoxins." In Cyanobacteria, 257–68. Chichester, UK: John Wiley & Sons, Ltd, 2013. http://dx.doi.org/10.1002/9781118402238.ch16.

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Catarina Guedes, A., Nadpi G. Katkam, João Varela, and Francisco Xavier Malcata. "Photobioreactors for cyanobacterial culturing." In Cyanobacteria, 270–92. Chichester, UK: John Wiley & Sons, Ltd, 2013. http://dx.doi.org/10.1002/9781118402238.ch17.

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Тези доповідей конференцій з теми "Cyanobacteria"

Gerasimenko, Lyudmila M., Georgi A. Zavarzin, Alexei Y. Rozanov, and Galina T. Ushatinskaya. "Cyanobacterial mats and mineralization of cyanobacteria." In SPIE's International Symposium on Optical Science, Engineering, and Instrumentation, edited by Richard B. Hoover. SPIE, 1998. http://dx.doi.org/10.1117/12.319850.

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Teneva, Ivanka, Dzhemal Moten, Detelina Belkinova, Tsvetelina Mladenova, and Balik Dzhambazov. "TOXIC POTENTIAL OF ANABAENOPSIS ELENKINII (CYANOBACTERIA) ISOLATED FROM A BLOOM IN LAKE VAYA (BULGARIA)." In 23rd SGEM International Multidisciplinary Scientific GeoConference 2023. STEF92 Technology, 2023. http://dx.doi.org/10.5593/sgem2023/5.1/s20.36.

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Cyanobacteria are ancient photosynthetic organisms that under certain conditions (high temperature, eutrophication) can grow rapidly and form "cyanobacterial blooms". Very often, these blooms are accompanied by production of cyanotoxins, which in most cases are dangerous to the other organisms. Knowing the producers of cyanotoxins is of utmost importance, especially today when climate change has been shown to increase the frequency of toxic cyanobacterial blooms worldwide. The aim of the present study was to characterize the toxic potential of the cyanobacterium Anabaenopsis elenkinii V.V.Miller 1923 isolated from Lake Vaia during a cyanobacterial bloom. The isolated strain (Anabaenopsis elenkinii) was identified based on a morphological analysis using light microscopy, and its taxonomic affiliation and phylogenetic position were confirmed by applying the Maximum Likelihood (ML) method for phylogenetic analysis based on the 16S rDNA sequence. The production of cyanotoxins was analyzed by applying immunological methods (ELISA) for detection of microcystins, cylindrospermopsin and saxitoxins. The toxic potential of Anabaenopsis elenkinii was evaluated in vitro by methyl-thiazole-tetrazolium (MTT) cytotoxicity and superoxide dismutase (SOD) activity assays using HT-29 cells. Our analyzes indicated that Anabaenopsis elenkinii produces microcystins (0.42 ng/mL), cylindrospermopsin (0.10 ng/mL) and saxithixins (0.05 ng/mL). The MTT cytotoxicity assay showed that the medium, in which the cyanobacterial strain was grown, significantly reduced the viability of HT-29 cells and this effect was dose- and time-dependent. In addition, 50% inhibition of the SOD activity was also observed. This is the first report of Anabaenopsis elenkinii as a producer of cyanotoxins. Our results provide valuable information about the toxin-producing cyanobacteria. They demonstrate the potential danger of "cyanobacterial blooms" where Anabaenopsis elenkinii is a dominant species.

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Jain, Aadhar, Erica E. Jung, Michael Kalontarov, and David Erickson. "Thermal and Optical Analysis of a Stacked Photobioreactor Design." In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-66263.

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In this work, we present thermal and optical analysis of a stacked photobioreactor design for growth of fuel producing photosynthetic cyanobacteria to achieve significantly higher volume and energy efficiency as compared to traditional photobioreactor designs. Our photobioreactor design incorporates racks of propagating slab waveguides [1], stacked over each other with spacing of a few hundred microns, in order to optimize light, fluid and gas delivery — the three essential ingredients for cyanobacterial growth — to the cyanobacteria growing in between the racks. The use of propagating slab waveguides provides a mechanism for efficient localized delivery of light to the cyanobacteria. However, it is important to analyze the light distribution of such waveguide systems in the photobioreactors to ensure they always remain within the optimal range for the bacteria. Further, the close packing of cyanobacteria in a closed system raises concerns regarding heat entrapment within the reactor, due to the heat produced as waste by the cyanobacteria. Higher temperatures can lead to a significant loss in efficiency in fuel producing and growth centers of the bacteria. Therefore it is important to design the reactor with appropriate thermal conditions for constraining the temperatures within optimal range for the bacteria. Here we attempt to simulate the thermal characteristics of such a system and estimate the temperature map of the system, and use these to dictate the design parameters and characteristics of the photobioreactor.

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Bataeva, Yulia, Lilit Grigoryan, Andrey Sorokin, and Olga Novichenko. "Study of the cyanobacteria effect on increasing in the rate of soil fertility in the arid zone." In "The Caspian in the Digital Age" within the framework of the International Scientific Forum "Caspian 2021: Ways of Sustainable Development". Dela Press Publishing House, 2022. http://dx.doi.org/10.56199/dpcsebm.adlz1478.

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We have studied the morphological and physicochemical characteristics of the soils of the Baer knolls in the Astrakhan region. Cyanobacterial communities were identified from the soil samples of the Baer knolls, the dominant forms of which was filamentous and heterocyst. The study of the cyanobacteria effect on increasing in the rate of fertility found that in all soil samples with cyanobacterial cultures was an increase the mass fraction of organic matter and pH of the water extract after three months of exposure.

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Vourc’h, Thomas, Julien Léopoldès, Annick Méjean, and Hassan Peerhossaini. "Motion of Active Fluids: Diffusion Dynamics of Cyanobacteria." In ASME 2016 Fluids Engineering Division Summer Meeting collocated with the ASME 2016 Heat Transfer Summer Conference and the ASME 2016 14th International Conference on Nanochannels, Microchannels, and Minichannels. American Society of Mechanical Engineers, 2016. http://dx.doi.org/10.1115/fedsm2016-7526.

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Cyanobacteria are photosynthetic micro-organisms colonizing all aquatic and terrestrial environments. The motility of such living micro-organisms should make their diffusion distinct from typical Brownian motion. This diffusion can be investigated in terms of global behavior (Fickian or not) and in terms of displacement probabilities, which provide more detail about the motility process. Using cyanobacterium Synechocystis sp. PCC 6803 as the model micro-organism, we carry out time-lapse video microscopy to track and analyze the bacteria’s trajectories, from which we compute the mean-squared displacement (MSD) and the distribution function of displacement probabilities. We find that the motility of Synechocystis sp. PCC 6803 is intermittent: high-motility “run” phases are separated by low-motility “tumble” phases corresponding to trapped states. However, this intermittent motility leads to a Fickian diffusive behavior, as shown by the evolution of the MSD with time.

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Berberoglu, Halil, Natasha Barra, Laurent Pilon, and Jenny Jay. "Growth CO2 Consumption, and H2 Production of Anabaena Variabilis ATCC 29413-U Under Different Irradiances and CO2 Concentrations." In ASME 2006 International Mechanical Engineering Congress and Exposition. ASMEDC, 2006. http://dx.doi.org/10.1115/imece2006-16144.

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Hydrogen production by cultivation of cyanobacteria in photobioreactors offers a clean and renewable alternative to thermochemical or electrolytic hydrogen production technologies with the added advantage of CO2 mitigation. The objective of this study is to experimentally investigate the CO2 consumption, growth, and H2 production of the cyanobacteria Anabaena variabilis ATCC 29413-U under atmosphere containing argon and CO2. Parameters investigated are irradiance and initial CO2 mole fraction in the gas phase. The CO2 consumption half-time, defined as the time at which the CO2 concentration in the gas phase decreases to half of its initial value, appears to be an appropriate time scale for modeling cyanobacterial CO2 consumption, growth, and H2 production. The half-time depends on both the initial CO2 mole fraction and the irradiance. Also, two regimes of growth have been identified depending on irradiance. Below 5,000 lux, the irradiance and the initial CO2 mole fraction have a coupled effect on cyanobacterial growth. Above 5,000 lux, growth depends only on the initial CO2 mole fraction. Furthermore, the optimum initial CO2 mole fraction around 0.05 has been identified for maximum growth and CO2 consumption rates. The growth and CO2 consumption were not inhibited by irradiance up to about 16,000 lux. Finally, the proposed empirical models can be used in conjunction with mass transfer and light transfer models to design and optimize the operating conditions of a photobioreactor for maximum hydrogen production and/or CO2 consumption.

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Zorina, A. A. "Protein kinases in cyanobacteria." In IX Congress of society physiologists of plants of Russia "Plant physiology is the basis for creating plants of the future". Kazan University Press, 2019. http://dx.doi.org/10.26907/978-5-00130-204-9-2019-182.

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Kouzminov, Fyodor I., Eugeny G. Maximov, Maxim Y. Gorbunov, and Victor V. Fadeev. "Fluorescent diagnostics of cyanobacteria." In SPIE Photonics Europe, edited by Jürgen Popp, Wolfgang Drexler, Valery V. Tuchin, and Dennis L. Matthews. SPIE, 2010. http://dx.doi.org/10.1117/12.854028.

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Silverman, Shaelyn N., Sebastian Kopf, Sanjoy Som, Brad M. Bebout, and Richard Gordon. "MEASURING N2 PRESSURE USING CYANOBACTERIA." In GSA Annual Meeting in Seattle, Washington, USA - 2017. Geological Society of America, 2017. http://dx.doi.org/10.1130/abs/2017am-299364.

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KIEŁBASA, SZYMON M., HANSPETER HERZEL, and ILKA M. AXMANN. "REGULATORY ELEMENTS OF MARINE CYANOBACTERIA." In Proceedings of the 7th Annual International Workshop on Bioinformatics and Systems Biology (IBSB 2007). IMPERIAL COLLEGE PRESS, 2007. http://dx.doi.org/10.1142/9781860949920_0001.

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Звіти організацій з теми "Cyanobacteria"

Tarachiu, Alexandru. Cyanobacteria and their uses. ResearchHub Technologies, Inc., September 2023. http://dx.doi.org/10.55277/researchhub.bmbtgw2j.

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McQueen, Andrew, Alyssa Calomeni-Eck, Ciera Kinley-Baird, Elizabeth Smith, Gerard Clyde, and Marvin Boyer. Management strategy for overwintering cyanobacteria in sediments contributing to harmful algal blooms (HABs). Engineer Research and Development Center (U.S.), May 2024. http://dx.doi.org/10.21079/11681/48472.

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Cyanobacteria that cause harmful algal blooms (HABs) can overwinter in sediments as resting cells (akinetes or vegetative colonies) and contribute to seasonal bloom resurgences. However, to date there has been limited focus on management tactics specifically targeting the control of cyanobacterial sources from sediments. Targeting resting cells in sediments for preventative management may provide a viable approach to delay onset and mitigate blooms (Calomeni et al. 2022). However, there are limited resources for this novel strategy. Given the growing global impact of HABs, there is a need to develop management strategies focused on sediments as a potential source and contributor to HABs. Therefore, the objective of this report is to provide a management strategy in terms of approaches, information, and case study examples for managing overwintering cyanobacteria in sediments with the goal of mitigating seasonal HAB occurrences.

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Pokrzywinski, Kaytee, Kaitlin Volk, Taylor Rycroft, Susie Wood, Tim Davis, and Jim Lazorchak. Aligning research and monitoring priorities for benthic cyanobacteria and cyanotoxins : a workshop summary. Engineer Research and Development Center (U.S.), August 2021. http://dx.doi.org/10.21079/11681/41680.

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In 2018, the US Army Engineer Research and Development Center partnered with the US Army Corps of Engineers–Buffalo District, the US Environmental Protection Agency, Bowling Green State University, and the Cawthron Institute to host a workshop focused on benthic and sediment-associated cyanobacteria and cyanotoxins, particularly in the context of harmful algal blooms (HAB). Technical sessions on the ecology of benthic cyanobacteria in lakes and rivers; monitoring of cyanobacteria and cyanotoxins; detection of benthic and sediment-bound cyanotoxins; and the fate, transport, and health risks of cyanobacteria and their associated toxins were presented. Research summaries included the buoyancy and dispersal of benthic freshwater cyanobacteria mats, the fate and quantification of cyanotoxins in lake sediments, and spatial and temporal variation of toxins in streams. In addition, summaries of remote sensing methods, omic techniques, and field sampling techniques were presented. Critical research gaps identified from this workshop include (1) ecology of benthic cyanobacteria, (2) identity, fate, transport, and risk of cyanotoxins produced by benthic cyanobacteria, (3) standardized sampling and analysis protocols, and (4) increased technical cooperation between government, academia, industry, nonprofit organizations, and other stakeholders. Conclusions from this workshop can inform monitoring and management efforts for benthic cyanobacteria and their associated toxins.

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Ruffing, Anne, Christine Alexandra Trahan, and Howland D. T. Jones. Genetic engineering of cyanobacteria as biodiesel feedstock. Office of Scientific and Technical Information (OSTI), January 2013. http://dx.doi.org/10.2172/1088046.

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Matthew Pandelakis, Matthew Pandelakis. Can we trick cyanobacteria into growing faster? Experiment, September 2014. http://dx.doi.org/10.18258/3496.

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Hutchins, David. Nitrogen and iron interactions in filamentous cyanobacteria. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.5817.

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Overman, Corina. Modeling Vertical Migration of Cyanobacteria and Zooplankton. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.7054.

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Davis, Ryan. Developing an Efficient Cyanobacteria Sugar Production Platform. Office of Scientific and Technical Information (OSTI), December 2018. http://dx.doi.org/10.2172/1528990.

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Pokrzywinski, Kaytee, Cliff Morgan, Scott Bourne, Molly Reif, Kenneth Matheson, and Shea Hammond. A novel laboratory method for the detection and identification of cyanobacteria using hyperspectral imaging : hyperspectral imaging for cyanobacteria detection. Engineer Research and Development Center (U.S.), June 2021. http://dx.doi.org/10.21079/11681/40966.

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To assist US Army Corps of Engineers resource managers in monitoring for cyanobacteria bloom events, a laboratory method using hyperspectral imaging has been developed. This method enables the rapid detection of cyanobacteria in large volumes and has the potential to be transitioned to aerial platforms for field deployment. Prior to field data collection, validation of the technology in the laboratory using monocultures was needed. This report describes the development of the detection method using hyperspectral imaging and the stability/reliability of these signatures for identification purposes. Hyperspectral signatures of different cyanobacteria were compared to evaluate spectral deviations between genera to assess the feasibility of using this imaging method in the field. Algorithms were then developed to spectrally deconvolute mixtures of cyanobacteria to determine relative abundances of each species. Last, laboratory cultures of Microcystis aeruginosa and Anabaena sp. were subjected to varying macro (nitrate and phosphate) and micro-nutrient (iron and magnesium) stressors to establish the stability of signatures within each species. Based on the findings, hyperspectral imaging can be a valuable tool for the detection and monitoring of cyanobacteria. However, it should be used with caution and only during stages of active growth for accurate identification and limited interference owing to stress.

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Carmichael, Wayne W. Freshwater Cyanobacteria (Blue-Green Algae) Toxins: Isolation and Characterization. Fort Belvoir, VA: Defense Technical Information Center, October 1985. http://dx.doi.org/10.21236/ada180183.

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