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Автор: Grafiati
Опубліковано: 14 березня 2023

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Статті в журналах з теми "Brightway"

1

Mutel, Chris. "Brightway: An open source framework for Life Cycle Assessment." Journal of Open Source Software 2, no. 12 (April 19, 2017): 236. http://dx.doi.org/10.21105/joss.00236.

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Steubing, Bernhard, Daniel de Koning, Adrian Haas, and Christopher Lucien Mutel. "The Activity Browser — An open source LCA software building on top of the brightway framework." Software Impacts 3 (February 2020): 100012. http://dx.doi.org/10.1016/j.simpa.2019.100012.

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Rosenstein, Jeffrey M. "Milton W. Brightman." Experimental Neurology 142, no. 1 (November 1996): 1–3. http://dx.doi.org/10.1006/exnr.1996.0172.

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Feder, Toni. "SPEAR3 Pierces Brightly." Physics Today 57, no. 3 (March 2004): 34–35. http://dx.doi.org/10.1063/1.4796428.

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5

Shaw, Dash. "Go Burn Brightly." American Book Review 36, no. 2 (2015): 4. http://dx.doi.org/10.1353/abr.2015.0005.

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6

Szuromi, P. D. "CHEMISTRY: Binding Be Brightly." Science 305, no. 5684 (July 30, 2004): 577b. http://dx.doi.org/10.1126/science.305.5684.577b.

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7

Blain, Andrew. "Through a glass brightly." Nature 393, no. 6685 (June 1998): 520–21. http://dx.doi.org/10.1038/31107.

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8

Bradley, Donal. "Plastic lasers shine brightly." Nature 382, no. 6593 (August 1996): 671. http://dx.doi.org/10.1038/382671a0.

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Turner, Edwin L. "Through a lens brightly." Nature 417, no. 6892 (June 2002): 905–6. http://dx.doi.org/10.1038/417905a.

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Claridge, John. "Through the glass brightly." Physics World 4, no. 2 (February 1991): 34–35. http://dx.doi.org/10.1088/2058-7058/4/2/19.

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Дисертації з теми "Brightway"

1

Ferrara, Nicola. "Atmospheric emissions profiles of geothermal energy production to minimise the environmental footprint: an innovative methodological investigation based on LCA approach." Doctoral thesis, Università di Siena, 2021. http://hdl.handle.net/11365/1126994.

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The fight against the climate change is the biggest challenge of the 21st century. The keyword in this context is “decarbonisation”: the development and management of an economic system with low Greenhouses Gases emissions. In this thesis, the environmental assessment of power generation technologies exploiting a renewable and environmentally friendly energy source, namely the geothermal energy, is presented. To evaluate the advantages and hot-spots of implementing such technologies the Life Cycle Assessment method was employed as the most powerful analytical tool for environmental sustainability analysis with a life cycle approach. With this method, it is possible to investigate a system with a multi-criteria approach, not limited to climate change only, but overarching a wide portfolio of environmental impact categories and indicators such as acidification, use of fresh water and depletion of natural resources. A rigorous scientific analysis should always be based on high-quality and robust data. Therefore, the atmospheric emissions of all the geothermal plants currently operating in Italy were carefully collected and analysed. In addition to direct emissions information, an extensive work to build the complete Life Cycle inventory of a state-of-the-art geothermal power plant. From the methodological point of view, the application of the Life Cycle Assessment to geothermal power plants allowed to point out a critical aspect method itself. To this purpose, special relevance in this work is given to the results related to the toxicity issue because they are the ones that attract the most public opinion and at the same time are more uncertain and unreliable. To improve the dissemination of LCA results and the diffusion of correct information, an essential step could be the implementation of methodological advances that facilitate the use LCA. In this work the development of simplified LCA models is presented and discussed in terms of the support that can provide both for decision-makers in their function, but also for experienced LCA practitioners.
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2

Gillies, Robert A. "Person and experience : a study in the thought of Edgard Sheffield Brightman." Thesis, University of St Andrews, 1990. http://hdl.handle.net/10023/6448.

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This dissertation comprises exegesis and critique of the personalistic idealism of Edgar Sheffield Brightman (1884 - 1953). Chapter One offers a biography of Brightman's life and identifies the intellectual heritage out of which he emerged and in which his thought may be situated. Chapters Two to Seven contain major exegesis of Brightman's metaphysical system relevant to the detailed critique and redefinition of person which follows in the remaining two Chapters. In these chapters the implications of Brightman's account of experience are examined extensively. The critique develops from relatively minor difficulties to a comprehensive analysis of the relationship that holds between the way he viewed experience and the category of substance. The argument will show that there is a distinction between experience and person which Brightman failed to recognize. The person is discovered to be (what will be called) a functional unity of purposive will and body with experience being one dimension of the person rather than its defining criterion. The concept of person as a functional unity of purposive will and responsive body with experience being a dimension in the person is found to be a more consistent outworking of Brightman's experiential foundation than his own bifurcation of person and body.
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Meerbach, Christian, Remo Tietze, Sascha Voigt, Vladimir Sayevich, Volodymyr M. Dzhagan, Steven C. Erwin, Zhiya Dang, et al. "Brightly Luminescent Core/Shell Nanoplatelets with Continuously Tunable Optical Properties Title." Wiley VCH, 2019. https://tud.qucosa.de/id/qucosa%3A34602.

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A straightforward, rapid method to create colloidally stable and brightly luminescent core/shell CdSe-based nanoplatelets (NPLs) with fluorescence quantum yields (QYs) up to 50% is demonstrated. A layer-by-layer deposition technique based on a two-phase mixture ‒ consisting of a nonpolar phase which includes the NPLs, and a saturated ionic polar phase ‒ to separate the reagents and hinder the nucleation of the shell material is used. The deposition of the first sulfur layer leads to a significant red-shift (by more than 100 nm) of the optical absorption and emission of the NPLs. Hence, by varying either the sulfur precursor content or the reaction time one can precisely and continuously tune the absorption and emission maxima from 520 to 630 nm. This evolution of the absorption onset during the shell growth is explained quantitatively using density-functional theory and atomistic statistical simulations. The emission can be further enhanced by exposure of the NPL solution to ambient sunlight. Finally, it is demonstrated that the core/shell NPLs can be transferred from the organic solution to aqueous media with no reduction of their QY that opens the door to a broad range of practical applications.
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4

Ullman, Christopher Charles. "God's personhood and God's knowableness an evangelical critique of the religious epistemology of Edgar Sheffield Brightman /." Theological Research Exchange Network (TREN), 1994. http://www.tren.com.

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Sholto-Douglas, Alice. "“Brightly Colored Magic and Weird Worlds”: Sylvia Plath’s Creation of Personae Through Her Visual Poetics." Master's thesis, Faculty of Humanities, 2019. http://hdl.handle.net/11427/31237.

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Анотація:
In this dissertation, I extend existing acknowledgments of the impact of Sylvia Plath’s visual arts training on her writing in order to argue that her painterly sensibilities are central to her character construction. Specifically, I contend that Plath draws upon a set of visual techniques, which I categorise as hallucinations, mental images, dreams, blurriness, and visual-to-verbal re-inscription. The ability to control subjective experience through the imagination, a philosophy Plath discusses in her journals, acts as a framework for her narratives, and visual techniques become, for Plath’s personae, a method of manipulating their experiences through a blurring of the divide between individual imagination and subjective reality. Plath’s visual techniques further function to represent her personae’s psychic interiority in ways that not only illustrate the limits to expression of the traditionally literary but also offer a means of overcoming these limits through an alternative system of meaning-making. Thus, her personae’s agency exists at the level of form, through self representation that is not stymied by the limitations of the written word, as well as at the level of narrative, through her personae’s control of experience. Moreover, because these visual techniques appear frequently in narratives that are preoccupied with a gendered power dynamic, I contend that we should understand Plath’s moments of resistance to textual tradition as enabling her personae’s escape from patriarchal limitations to freedom and selfexpression. While this recognition of the significance of Plath’s visual techniques should not necessarily constitute a panacea to the constraints of traditional language, it does offer a new way of reading Plath which acknowledges her painterly sensibilities as crucial to the way in which she gives her personae agency and writes back to her literary forefathers.
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Crome, Andrew Peter. "The Jews and the Literal Sense : Hermeneutical Approaches in the Apocalyptic Commentaries of Thomas Brightman (1562-1607)." Thesis, University of Manchester, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.501973.

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Raevskaya, Alexandra, Vladimir Lesnyak, Danny Haubold, Volodymyr Dzhagan, Oleksandr Stroyuk, Nikolai Gaponik, Dietrich R. T. Zahn, and Alexander Eychmüller. "A Fine Size Selection of Brightly Luminescent Water-Soluble Ag-In-S and Ag-In-S/ZnS Quantum Dots." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-226647.

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A size-selected series of water-soluble luminescent Ag–In–S (AIS) and core/shell AIS/ZnS QDs were produced by a precipitation technique. Up to 10–11 fractions of size-selected AIS (AIS/ZnS) QDs emitting in a broad color range from deep-red to bluish-green were isolated with the photoluminescence (PL) quantum yield reaching 47% for intermediate fractions. The size of the isolated AIS (AIS/ZnS) QDs varied from ~2 nm to ~3.5 nm at a roughly constant chemical compo- sition of the particles throughout the fractions as shown by the X-ray photoelectron spectroscopy. The decrease of the mean AIS QD size in consecutive fractions was accompanied by an increase of the structural QD imperfection/disorder as deduced from a notable Urbach absorption “tail” below the fundamental absorption edge. The Urbach increased from 90–100 meV for the largest QDs up to 350 meV for the smallest QDs, indicating a broadening of the distribution of sub-bandgap states. Both the Urbach energy and the PL bandwidth of the size-selected AIS QDs increased with QD size reduction from 3–4 nm to ~2 nm and a distinct correlation was observed between these parameters. A study of size-selected AIS and AIS/ZnS QDs by UV photoelectron spectroscopy on Au and FTO substrates revealed their valence band level EVB at ~6.6 eV (on Au) and ~7 eV (on FTO) and pinned to the Fermi level of conductive substrates resulting in a masking of any possible size- dependence of the valence band edge position.
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Raithel, Jutta C. "In a mirror, brightly, the didactic value of visual imagery in an early icon and an ekphrastic sermon (the Enthroned Virgin of Mount Sinai and Pseudo-Gregory of Nazianzus, Oration 35)." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/mq43400.pdf.

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9

Yong, Amos. "From Pietism to Pluralism: Boston Personalism and the Liberal Era in American Methodist Theology, 1876-1953." PDXScholar, 1995. https://pdxscholar.library.pdx.edu/open_access_etds/3089.

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Boston personalism has generally been recognized as a philosophic system based upon a metaphysical idealism. What is less known, however, is that the founder of this school of thought and some of the major contributors to the early development of this tradition were committed members of the Methodist Episcopal Church. The purpose of this study is to examine the contributions made by the early Boston personalists to the cause of theological liberalism in the Methodist Church. It will be shown that personalist philosophers and theologians at Boston University ushered in and consolidated the liberal era in Methodist theology. Further, it will be argued that the religious demands of the philosophy of personalism eventually led some members of the tradition from theological liberalism to modernism and the beginnings of a religious pluralism. In other words, the thesis of this study is that the early Boston personalists were theological innovators in the Methodist Church, leading the denomination from its nineteenth-century evangelical pietism to the modernism and pluralism that was part of mid-twentieth century American Protestantism. The focus of this study will therefore be on the first two generations of personalists at Boston University: the founder of the personalist tradition, Borden Parker Bowne, and two of his most prominent students, Albert Cornelius Knudson and Edgar Sheffield Brightman. One chapter is devoted to each of figure, focused upon the impact of their personalist philosophy and methodology on their theology and philosophy of religion, and their influence on American Methodist theology. The period this study, which commences from the time of Bowne's appointment to the Department of Philosophy at Boston University in 1876 to the death of both Knudson and Brightman in 1953, reveals how Methodism grappled with the theological implications raised by the complexities of modernity and the emerging sciences. Attention will be focused on how the philosophical method of the personalists dictated their movement from pietism toward liberalism and onto modernism and pluralism. As such, this study demonstrates the integral role played by the Boston personalist tradition in theological development during the liberal era of American Methodism.
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Lin, Jeng-yi, and 林政毅. "The Effect of Habitual Domains Brightly Attitudes on Happiness." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/12417908583599548387.

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Анотація:
碩士
大同大學
資訊經營學系(所)
103
“All has occurred the matter, all is helps us to grow” all things encourages the faith, eagerly anticipates the opening life another leaf of window. Happiness is the joyful springhead, but the happy heart comes from the intrinsic self-subjective thought to feel the positive and negative mood psychology intensity to life all around. It is impossible to create a first-class enterprise if there are no happy employees. The main purpose of the current study is to explore the relationship between bright attitudes of the habitual domains and happiness of employees in enterprises, and to investigate which bright attitudes have significant effect on employees’ happiness. Originates mood of psychology faith by seven bright attitudes of the habitual domains, thorough understanding bright attitudes of view psychology faith to the staff happiness positive influence. And penetrates “the bright attitudes of the habitual domains meter” and “the happiness meter” two meters hands over by receives measuring to survey, the discussion custom domain bright point of view to the happiness the influence, then analyzes both the correlation degree. This research picks the network questionnaire survey to carry on the material collection, transmits in office jobholders primarily, total takes back five hundred effective questionnaire. The results indicated that the happiness can be categorized as four dimensions: self-respect and satisfaction, harmonious relationship, positive emotion, and optimism and joyfulness. The happiness of “harmonious relationship” was significantly positive affected by seven bright attitudes of the habitual domains: respectful life, everything helps growth, objectives with understanding and practice, duties and master, mission and joyful field, gratitude and charity, and enjoyment and devotion. All the seven bright attitudes had the effect on the happiness of “positive emotion”; within these attitudes, “gratitude and charity” had significantly negative effect but the other six attitudes had significantly positive effect on the “positive emotion”. Besides “gratitude and charity”, the other six attitudes also had significantly positive effect on the happiness of “optimism and joyfulness”. The happiness of “self-respect and satisfaction” was affected by the attitudes of “mission and joyful field”, “objectives with understanding and practice”, “everything helps growth” and “respectful life”. These results also suggest that the bright attitudes of the habitual domains could become the major source of people’s happiness in their daily life. Fulfills in our daily life, in other words, is familiar with the bright attitudes of the habitual domains is the people lives the happiness springhead and the origin, is maintains the happiness extension the kinetic energy faith element.
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Книги з теми "Brightway"

1

Bracken, Alexandra. Brightly woven. New York: Egmont USA, 2010.

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2

Bracken, Alexandra. Brightly woven. New York: Egmont USA, 2010.

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3

Lackey, Mercedes. Brightly burning. New York: Daw, 2000.

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4

Bracken, Alexandra. Brightly woven. New York: Egmont USA, 2010.

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5

Bracken, Alexandra. Brightly woven. New York, NY: Egmont USA, 2011.

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6

Lackey, Mercedes. Brightly Burning. New York: Penguin USA, Inc., 2009.

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7

Donne, Alexa. Brightly burning. Boston: Houghton Mifflin Harcourt, 2018.

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8

Lackey, Mercedes. Brightly burning. New York: DAW Books, 2000.

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9

Bracken, Alexandra. Brightly woven. New York: Egmont USA, 2010.

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10

Animals brightly colored. Watertown, MA: Charlesbridge, 1998.

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Частини книг з теми "Brightway"

1

Vishveshwara, C. V. "Through the Glass Brightly." In Astronomers' Universe, 147–57. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-08213-4_12.

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2

Crome, Andrew. "Thomas Brightman and Judeo-Centrism 1610–1640." In The Restoration of the Jews: Early Modern Hermeneutics, Eschatology, and National Identity in the Works of Thomas Brightman, 131–65. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-04762-1_5.

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Crome, Andrew. "Historicising Allegory: Thomas Brightman and the Historicising Commentary." In The Restoration of the Jews: Early Modern Hermeneutics, Eschatology, and National Identity in the Works of Thomas Brightman, 59–95. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-04762-1_3.

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4

Geldhof, John G., Svea G. Olsen, and Asia A. Thogmartin. "The Morning Sun Shines Brightly: Positive Youth in a Global." In Springer Series on Child and Family Studies, 567–77. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-70262-5_37.

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Weisl, Angela Jane. "“She Appears as Brightly Radiant as She Once was Foul”: Medieval Conversion Narratives and Contemporary Makeover Shows." In Cultural Studies of the Modern Middle Ages, 47–61. New York: Palgrave Macmillan US, 2007. http://dx.doi.org/10.1057/9780230610040_3.

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Crome, Andrew. "Introduction." In The Restoration of the Jews: Early Modern Hermeneutics, Eschatology, and National Identity in the Works of Thomas Brightman, 1–28. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-04762-1_1.

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Crome, Andrew. "Hermeneutics and the Jews in Protestant Thought." In The Restoration of the Jews: Early Modern Hermeneutics, Eschatology, and National Identity in the Works of Thomas Brightman, 29–58. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-04762-1_2.

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"Portrait: Edgar Sheffield Brightman." In American Philosophical Association Centennial Series, 404. Philosophy Documentation Center, 2013. http://dx.doi.org/10.5840/apapa2013642.

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Hull, Richard T. "Biography: Edgar Sheffield Brightman." In American Philosophical Association Centennial Series, 405–9. Philosophy Documentation Center, 2013. http://dx.doi.org/10.5840/apapa2013643.

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Cooper, David. "‘Stars, stars, brightly shine’." In Béla Bartók, 276–308. Yale University Press, 2015. http://dx.doi.org/10.12987/yale/9780300148770.003.0010.

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Тези доповідей конференцій з теми "Brightway"

1

Petrov, Valery. "Silver halide holograms and HOEs obtained in a brightly lit environment." In Optoelectronics and High-Power Lasers & Applications, edited by Ivan Cindrich and Sing H. Lee. SPIE, 1998. http://dx.doi.org/10.1117/12.310587.

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Yersin, Hartmut, Rafal Czerwieniec, and Alexander Hupfer. "Singlet harvesting with brightly emitting Cu(I) and metal-free organic compounds." In SPIE Photonics Europe, edited by Barry P. Rand, Chihaya Adachi, and Volker van Elsbergen. SPIE, 2012. http://dx.doi.org/10.1117/12.921372.

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3

Zhang, Feng, Cheng Chen, Xiangang Wu, Xiangmin Hu, Hailong Huang, Junbo Han, Bingsuo Zou, Yuping Dong, and Haizheng Zhong. "Brightly-Luminescent and Color-Tunable Colloidal CH3NH3PbX3 (X=Br, I, Cl) Quantum Dots: Potential Alternatives for Display Technology." In Photonics for Energy. Washington, D.C.: OSA, 2015. http://dx.doi.org/10.1364/pfe.2015.pw2e.3.

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Su, Qingzhi, Chaoyang Chai, and Feng Zhao. "Synthesis and Photophysical Properties of Four-Coordinate N-heterocyclic Carbene Copper(Ⅰ) Complex Emitting Material with Brightly Luminescence." In 2018 7th International Conference on Energy and Environmental Protection (ICEEP 2018). Paris, France: Atlantis Press, 2018. http://dx.doi.org/10.2991/iceep-18.2018.277.

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Reeves, Adam, James Schirillo, and Lawrence Arend. "Depth, lightness, and brightness of ach romatic surfaces." In OSA Annual Meeting. Washington, D.C.: Optica Publishing Group, 1987. http://dx.doi.org/10.1364/oam.1987.thc3.

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Анотація:
Gilchrist’s1 subjects saw a test paper as adjacent to either a paper in a distant brightly lit room or a second paper in a closer dimly lit room (the apparent depth of the test being determined by interposition cues). The test and two other papers were always retinally adjacent. Gilchrist reported that grey-scale matches to the test were strongly influenced by the apparently coplanar paper; the other noncoplanar paper had virtually no effect. We wondered what Gilchrist's subjects had matched: brightness or lightness. We accurately simulated the rooms (excluding shadows) on a high-quality monitor. Luminance ratios (unlike Gilchrist's) under each illuminant were natural, not exceeding 30:1. Lightness matches showed Gilchrist's effect but to a lesser degree whether depth was cued by interposition alone or by stereo in addition. Brightness matches showed no effect of perceived depth. Results were replicated with a method of adjustment.
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Gordon, James, and Israel Abramov. "Scaling Procedures for Specifying Color Appearance." In Color Appearance. Washington, D.C.: Optica Publishing Group, 1987. http://dx.doi.org/10.1364/ca.1987.ma3.

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The world around us is often brightly colored. For various reasons it may be necessary to specify these colors precisely. One way is to describe each color in terms of the mixture of three primaries needed to match it. But this tells us only about equivalences and not about appearance. Furthermore, colorimetric matches remain stable even though appearances can change drastically with viewing conditions (1;2). We propose that hue and saturation scaling can be used to specify appearance precisely and reliably. The general techniques have been described earlier (3-5). We have examined several methodological variants to find one that is easily and reliably used, obtained "standard" functions from large groups of subjects under a variety of viewing conditions, and have tested the applicability of the technique with untrained observers. We have also used multidimensional scaling (MDS) (6;7) to derive uniform appearance diagrams (UAD) from the scaling data; from these spaces we have derived discrimination functions.
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Swihart, Mark T., Xuegeng Li, Yuanqing He, William D. Kirkey, Alexander N. Cartwright, Yudhisthira Sahoo, and Paras N. Prasad. "High-rate synthesis and characterization of brightly luminescent silicon nanoparticles with applications in hybrid materials for photonics and biophotonics." In Optical Science and Technology, SPIE's 48th Annual Meeting, edited by David L. Andrews, Zeno Gaburro, Alexander N. Cartwright, and Charles Y. C. Lee. SPIE, 2003. http://dx.doi.org/10.1117/12.509337.

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8

Schirillo, James A., and Steven K. Shevell. "Perceived brightness, but not lightness, is influenced by retinally non-adjacent coplanar surfaces." In OSA Annual Meeting. Washington, D.C.: Optica Publishing Group, 1992. http://dx.doi.org/10.1364/oam.1992.tuc4.

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We previously reported (OSA, 1991; ARVO, 1992) that perceived brightness of an achromatic surface is influenced by information about the illuminant derived from perceptually coplanar but retinally noncontiguous surfaces. A 6:1 luminance range on a CRT screen simulated the surface reflectances of a single Mondrian. Corresponding spatial locations in a second Mondrian were assigned luminances that were one-fifth that of the first Mondrian. This created the appearance of two Mondrians with identical reflectances, but different illuminations. When viewed through a haploscope each Mondrian was perceived in a separate stereoscopic depth plane. The test appeared in either the depth plane of the far, dimly illuminated Mondrian or the depth plane of the near, brightly illuminated Mondrian. The previous results confound changes of reflectance and illumination. We obtained lightness judgments by using the same stimuli to isolate perceived reflectance. The results show that brightness of the test changed with depth, but not lightness. This supports the claim that brightness measures are due to perceived illumination differences across depth planes.
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9

Yersin, Hartmut, Markus J. Leitl, and Rafal Czerwieniec. "TADF for singlet harvesting: next generation OLED materials based on brightly green and blue emitting Cu(I) and Ag(I) compounds." In SPIE Organic Photonics + Electronics, edited by Franky So and Chihaya Adachi. SPIE, 2014. http://dx.doi.org/10.1117/12.2061010.

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10

Hantgan, R. R. "LOCALIZATION OF THE DOMAINS OF FIBRIN INVOLVED IN BINDING TO PLATELETS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643773.

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The molecular basis of platelet-fibrin interactions has been investigated by using synthetic peptides as potential inhibitors of binding fibrin protofibrils and fibrinogen to ADP-stimulated platelets, adhesion of fibrin fibers to the platelet surface, and platelet-mediated clot retraction. Synthetic peptides RGDS and HHLGGAKQAGDV, corresponding to regions of the fibrinogen α and γ chains previously identified as platelet recognition sites, inhibited the binding of radiolabelled soluble fibrin oligomers to ADP-stimulated platelets with IC50 values of 12 and 40 μM, respectively. The IC50 values obtained with fibrinogen as the ligand were 3-fold higher. Synthetic GPRP and GHRP, corresponding to the N-terminal sequences of the fibrin α and β chains, were minimally effective in blocking soluble fibrin oligomer binding to ADP-stimulated platelets. The extent of fibrin:platelet adhesion was determined with a microfluorimetric technique which measures the quantity of fluorescein-labelled fibrin attached to the surface of platelets. The signal obtained from the brightly fluorescent platelet:fibrin adducts was time- and concentration-dependent, and was fully inhibited by a monoclonal antibody directed against the glycoprotein II:IIIa complex (HP1-1D, kindly provided by Dr. W. Nichols). Inhibition of fibrin:platelet adhesion by RGDS, HHGGAKQAGDV, and GHRP all exhibited a similar, linear dependence on the peptide concentration, reaching 1/2 maximum at about 200 μM, suggesting nonspecific effects. GPRP inhibited fibrin assembly but did not appear to have specific effects on fibrin:platelet adhesion. The time course of clot retraction was followed by right angle light scattering intensity measurements. Only RGDS affected clot retraction, causing a 4-fold decrease in rate at 230 μM. These results indicate that fibrinogen and fibrin protofibrils, which are obligatory intermediates in the fibrin assembly pathway, share a set of common platelet recognition sites located at specific regions of the α and γ chains of the multinodular fibrin(ogen) molecules. The RGDS site is also involved in mediating interactions between the three dimensional fibrin network and ADP-stimulated platelets.
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Звіти організацій з теми "Brightway"

1

Bostock, Richard M., Dov Prusky, and Martin Dickman. Redox Climate in Quiescence and Pathogenicity of Postharvest Fungal Pathogens. United States Department of Agriculture, May 2003. http://dx.doi.org/10.32747/2003.7586466.bard.

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Monilinia fructicola causes brown rot blossom blight and fruit rot in stone fruits. Immature fruit are highly resistant to brown rot but can become infected. These infections typically remain superficial and quiescent until they become active upon maturation of the fruit. High levels of chlorogenic acid (CGA) and related compounds occur in the peel of immature fruit but these levels decline during ripening. CGA inhibits cutinase expression, a putative virulence factor, with little or no effect on spore germination or hyphal growth. To better understand the regulation of cutinase expression by fruit phenolics, we examined the effect of CGA, caffeic acid (CA) and related compounds on the redox potential of the growth medium and intracellular glutathione (GSH) levels. The presence of CA in the medium initially lowered the electrochemical redox potential of the medium, increased GSH levels and inhibited cutinase expression. Conidia germinated in the presence of CA, CGA, or GSH produced fewer appressoria and had elongated germ tubes compared to the controls. These results suggest that host redox compounds can regulate fungal infectivity. In order to genetically manipulate this fungus, a transformation system using Agrobacterium was developed. The binary transformation vector, pPTGFPH, was constructed from the plasmid pCT74, carrying green fluorescent protein (GFP) driven by the ToxA promoter of Pyrenophora tritici-repentis and hygromycin B phosphotransferase (hph) under control of the trpC promoter of from Aspergillus nidulans, and the binary vector pCB403.2, carrying neomycin phosphotransferase (nptII) between the T-DNA borders. Macroconidia of M. fructicola were coincubated with A. tumefaciens strain LBA 4404(pPTGFPH) on media containing acetosyringone for two days. Hygromycin- and G418-resistant M. fructicola transformants were selected while inhibiting A. tumefaciens with cefotaxime. Transformants expressing GFP fluoresced brightly, and were formed with high efficiency and frequency of T-DNA integration frequency. The use of these transformants for in situ studies on stone fruit tissues is discussed.
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