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Добірка наукової літератури з теми "Algal Protein"

Автор: Grafiati
Опубліковано: 11 березня 2023

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Статті в журналах з теми "Algal Protein"

1

Hirakawa, Yoshihisa, Fabien Burki, and Patrick J. Keeling. "Genome-Based Reconstruction of the Protein Import Machinery in the Secondary Plastid of a Chlorarachniophyte Alga." Eukaryotic Cell 11, no. 3 (January 20, 2012): 324–33. http://dx.doi.org/10.1128/ec.05264-11.

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ABSTRACT Most plastid proteins are encoded by their nuclear genomes and need to be targeted across multiple envelope membranes. In vascular plants, the translocons at the outer and inner envelope membranes of chloroplasts (TOC and TIC, respectively) facilitate transport across the two plastid membranes. In contrast, several algal groups harbor more complex plastids, the so-called secondary plastids, which are surrounded by three or four membranes, but the plastid protein import machinery (in particular, how proteins cross the membrane corresponding to the secondary endosymbiont plasma membrane) remains unexplored in many of these algae. To reconstruct the putative protein import machinery of a secondary plastid, we used the chlorarachniophyte alga Bigelowiella natans , whose plastid is bounded by four membranes and still possesses a relict nucleus of a green algal endosymbiont (the nucleomorph) in the intermembrane space. We identified nine homologs of plant-like TOC/TIC components in the recently sequenced B. natans nuclear genome, adding to the two that remain in the nucleomorph genome ( B. natans TOC75 [BnTOC75] and BnTIC20). All of these proteins were predicted to be localized to the plastid and might function in the inner two membranes. We also show that the homologs of a protein, Der1, that is known to mediate transport across the second membrane in the several lineages with secondary plastids of red algal origin is not associated with plastid protein targeting in B. natans . How plastid proteins cross this membrane remains a mystery, but it is clear that the protein transport machinery of chlorarachniophyte plastids differs from that of red algal secondary plastids.
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2

Blaby-Haas, Crysten E., and Sabeeha S. Merchant. "Comparative and Functional Algal Genomics." Annual Review of Plant Biology 70, no. 1 (April 29, 2019): 605–38. http://dx.doi.org/10.1146/annurev-arplant-050718-095841.

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Over 100 whole-genome sequences from algae are published or soon to be published. The rapidly increasing availability of these fundamental resources is changing how we understand one of the most diverse, complex, and understudied groups of photosynthetic eukaryotes. Genome sequences provide a window into the functional potential of individual algae, with phylogenomics and functional genomics as tools for contextualizing and transferring knowledge from reference organisms into less well-characterized systems. Remarkably, over half of the proteins encoded by algal genomes are of unknown function, highlighting the volume of functional capabilities yet to be discovered. In this review, we provide an overview of publicly available algal genomes, their associated protein inventories, and their quality, with a summary of the statuses of protein function understanding and predictions.
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3

Sproles, Ashley E., Anthony Berndt, Francis J. Fields, and Stephen P. Mayfield. "Improved high-throughput screening technique to rapidly isolate Chlamydomonas transformants expressing recombinant proteins." Applied Microbiology and Biotechnology 106, no. 4 (February 2022): 1677–89. http://dx.doi.org/10.1007/s00253-022-11790-9.

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Abstract The single-celled eukaryotic green alga Chlamydomonas reinhardtii has long been a model system for developing genetic tools for algae, and is also considered a potential platform for the production of high-value recombinant proteins. Identifying transformants with high levels of recombinant protein expression has been a challenge in this organism, as random integration of transgenes into the nuclear genome leads to low frequency of cell lines with high gene expression. Here, we describe the design of an optimized vector for the expression of recombinant proteins in Chlamydomonas, that when transformed and screened using a dual antibiotic selection, followed by screening using fluorescence activated cell sorting (FACS), permits rapid identification and isolation of microalgal transformants with high expression of a recombinant protein. This process greatly reduces the time required for the screening process, and can produce large populations of recombinant algae transformants with between 60 and 100% of cells producing the recombinant protein of interest, in as little as 3 weeks, that can then be used for whole population sequencing or individual clone analysis. Utilizing this new vector and high-throughput screening (HTS) process resulted in an order of magnitude improvement over existing methods, which normally produced under 1% of algae transformants expressing the protein of interest. This process can be applied to other algal strains and recombinant proteins to enhance screening efficiency, thereby speeding up the discovery and development of algal-derived recombinant protein products. Key points • A protein expression vector using double-antibiotic resistance genes was designed • Double antibiotic selection causes fewer colonies with more positive for phenotype • Coupling the new vector with FACS improves microalgal screening efficiency > 60%
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4

K., Santhoshkumar, Prasanthkumar S., and J. G. Ray. "Chlorococcum humicola (Nageli) Rabenhorst as a Renewable Source of Bioproducts and Biofuel." Journal of Plant Studies 5, no. 1 (February 29, 2016): 48. http://dx.doi.org/10.5539/jps.v5n1p48.

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Анотація:
Among the diverse new generation biomass yielding species, green algae are the most promising organisms. Compared to biomass production of other organisms, production of algae is less laborious, quite fast, and more economical. Moreover, eutrophicated waters get naturally purified in the cultivation process of algae. Algal biomass from monoculture of specific species, which are rich in carbohydrates, proteins and lipids, is considered a good source of diverse bio-products and feed-stock for food, feeds and bio-fuels. Quantity and quality of algal biomass for specific products depend on the species and strains as well as environmental conditions of cultivation. In this connection, biomass productivity and oil-yield of a local strain of <em>Chlorococcum humicola </em>(Nageli) Rabenhorst was assessed in Bold’s Basal Medium. Long-term storage capacity of the alga was tried by entrapping the algal cells in sodium alginate beads, which showed viability up to 14 months. Estimation of total carbohydrate, protein, lipid and chemical characterization of oil as well as the feasibility of its conversion to biodiesel revealed the industrial potential of this local strain as a source of food and biofuel. Fatty acid profiling of the extracted oil showed that 70% are mono-saturated and 12.2 % are nutritionally important polyunsaturated fatty acids. The oil could be effectively trans-esterified to methyl esters and the conversion was confirmed by FTIR spectroscopy. Further standardization of the mass production of the alga in natural environmental conditions for biomass and oil is progressing to optimize its value as globally competent food, nutraceutical and biofuel resource.
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5

Zivanovic, Ana, and Danielle Skropeta. "c-AMP Dependent Protein Kinase a Inhibitory Activity of Six Algal Extracts from South Eastern Australia and Their Fatty Acid Composition." Natural Product Communications 7, no. 7 (July 2012): 1934578X1200700. http://dx.doi.org/10.1177/1934578x1200700731.

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c-AMP Dependent protein kinase (protein kinase A, PKA) is an important enzyme involved in the regulation of an increasing number of physiological processes including immune function, cardiovascular disease, memory disorders and cancer. The objective of this study was to evaluate the PKA inhibitory activity of a range of algal extracts, along with their fatty acid composition. Six algal species were investigated including two Chlorophyta ( Codium dimorphum and Ulva lactuca), two Phaeophyta ( Phyllospora comosa and Sargassum sp.) and two Rhodophyta ( Prionitis linearis and Corallina vancouveriensis), with the order of PKA inhibitory activity of their extracts identified as follows: brown seaweeds > red seaweeds > green seaweeds with the brown alga Sargassum sp. exhibiting the highest PKA inhibitory activity (84% at 100 μg/mL). GC/MS analysis identified a total of 18 fatty acids in the six algal extracts accounting for 72-87% of each extract, with hexadecanoic acid and 9,12-octadecadienoic acid as the dominant components. The most active extract ( Sargassum sp.) also contained the highest percentage of the saturated C14:0 fatty acid (12.8% of the total extract), which is a known to inhibit PKA. These results provide the first description of the PKA inhibitory activity of marine algae along with the first description of the fatty acid composition of these six algal species from South Eastern Australian waters. Importantly, this study reveals that abundant and readily available marine algae are a new and relatively unexplored source of PKA inhibitory compounds.
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6

Tipsukhon Pimpimol, Burassakorn Tongmee, Padivarada Lomlai, Prsert Prasongpol, Niwooti Whangchai, Yuwalee Unpaprom, and Rameshprabu Ramaraj. "Spirogyra cultured in fishpond wastewater for biomass generation." Maejo International Journal of Energy and Environmental Communication 2, no. 3 (December 31, 2020): 58–65. http://dx.doi.org/10.54279/mijeec.v2i3.245041.

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Анотація:
Algae are aquatic organisms that can be found in a wide range of water bodies. Algae, a form of aquatic organism, is found in many different water types. Besides being relatively easy to maintain, algae are also numerous, making them a good choice for biomass production. The filamentous Spirogyra sp., a common green alga, tends to grow in freshwater. It is said that this macroalga has a wide variety of biotechnological applications. Research in this area highlights biomass's creation and builds on our understanding of the composition of macroalgae generated in fish farm wastewater. A study of Spirogyra in undisturbed fish farm wastewater was conducted in this study. Various algal species were evaluated for their qualities, including biomass yields and productivity, protein, fat, and carbohydrates. This investigation has confirmed that the nutrients in fish farm effluent are suitable for cultivating algal biomass. Protein, lipid, and carbohydrate levels in unaltered fish farm effluent were the highest for Spirogyra, with percentages of 19.03, 8.38, and 45.71%, respectively. Thus, it was the most suitable organism for various biomass-based applications and nutrient removal.
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7

Cavalier-Smith, T. "Genomic reduction and evolution of novel genetic membranes and protein-targeting machinery in eukaryote-eukaryote chimaeras (meta-algae)." Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences 358, no. 1429 (January 29, 2003): 109–34. http://dx.doi.org/10.1098/rstb.2002.1194.

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Анотація:
Chloroplasts originated just once, from cyanobacteria enslaved by a biciliate protozoan to form the plant kingdom (green plants, red and glaucophyte algae), but subsequently, were laterally transferred to other lineages to form eukaryote–eukaryote chimaeras or meta–algae. This process of secondary symbiogenesis (permanent merger of two phylogenetically distinct eukaryote cells) has left remarkable traces of its evolutionary role in the more complex topology of the membranes surrounding all non–plant (meta–algal) chloroplasts. It took place twice, soon after green and red algae diverged over 550 Myr ago to form two independent major branches of the eukaryotic tree (chromalveolates and cabozoa), comprising both meta–algae and numerous secondarily non–photosynthetic lineages. In both cases, enslavement probably began by evolving a novel targeting of endomembrane vesicles to the perialgal vacuole to implant host porter proteins for extracting photosynthate. Chromalveolates arose by such enslavement of a unicellular red alga and evolution of chlorophyll c to form the kingdom Chromista and protozoan infrakingdom Alveolata, which diverged from the ancestral chromalveolate chimaera. Cabozoa arose when the common ancestor of euglenoids and cercozoan chlorarachnean algae enslaved a tetraphyte green alga with chlorophyll a and b . I suggest that in cabozoa the endomembrane vesicles originally budded from the Golgi, whereas in chromalveolates they budded from the endoplasmic reticulum (ER) independently of Golgi–targeted vesicles, presenting a potentially novel target for drugs against alveolate Sporozoa such as malaria parasites and Toxoplasma . These hypothetical ER–derived vesicles mediated fusion of the perialgal vacuole and rough ER (RER) in the ancestral chromist, placing the former red alga within the RER lumen. Subsequently, this chimaera diverged to form cryptomonads, which retained the red algal nucleus as a nucleomorph (NM) with approximately 464 protein–coding genes (30 encoding plastid proteins) and a red or blue phycobiliprotein antenna pigment, and the chromobiotes (heterokonts and haptophytes), which lost phycobilins and evolved the brown carotenoid fucoxanthin that colours brown seaweeds, diatoms and haptophytes. Chromobiotes transferred the 30 genes to the nucleus and lost the NM genome and nuclear–pore complexes, but retained its membrane as the periplastid reticulum (PPR), putatively the phospholipid factory of the periplastid space (former algal cytoplasm), as did the ancestral alveolate independently. The chlorarachnean NM has three minute chromosomes bearing approximately 300 genes riddled with pygmy introns. I propose that the periplastid membrane (PPM, the former algal plasma membrane) of chromalveolates, and possibly chlorarachneans, grows by fusion of vesicles emanating from the NM envelope or PPR. Dinoflagellates and euglenoids independently lost the PPM and PPR (after diverging from Sporozoa and chlorarachneans, respectively) and evolved triple chloroplast envelopes comprising the original plant double envelope and an extra outermost membrane, the EM, derived from the perialgal vacuole. In all metaalgae most chloroplast proteins are coded by nuclear genes and enter the chloroplast by using bipartite targeting sequences – an upstream signal sequence for entering the ER and a downstream chloroplast transit sequence. I present a new theory for the four–fold diversification of the chloroplast OM protein translocon following its insertion into the PPM to facilitate protein translocation across it (of both periplastid and plastid proteins). I discuss evidence from genome sequencing and other sources on the contrasting modes of protein targeting, cellular integration, and evolution of these two major lineages of eukaryote ‘cells within cells’. They also provide powerful evidence for natural selection's effectiveness in eliminating most functionless DNA and therefore of a universally useful non–genic function for nuclear non–coding DNA, i.e. most DNA in the biosphere, and dramatic examples of genomic reduction. I briefly argue that chloroplast replacement in dinoflagellates, which happened at least twice, may have been evolutionarily easier than secondary symbiogenesis because parts of the chromalveolate protein–targeting machinery could have helped enslave the foreign plastids.
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8

Vasileva, Iv, and J. Ivanova. "BIOCHEMICAL PROFILE OF GREEN AND RED ALGAE – A KEY FOR UNDERSTANDING THEIR POTENTIAL APPLICATION AS FOOD ADDITIVES." Trakia Journal of Sciences 17, no. 1 (2019): 1–7. http://dx.doi.org/10.15547/tjs.2019.01.001.

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Анотація:
PURPOSE: With the improvements of the microalgal cultivation industry, it became possible to add algal biomass and its metabolites in foods in order to create a balanced and health-food. METHODS: By determining the growth and the biochemical composition (lipids, carbohydrates and proteins), two algal strains were evaluated as a potential source of food additives – the newly isolated strain of the green algae Scenedesmus sp. and the red algae Porphyridium cruentum). RESULTS: It turned out that in Scenedesmus sp. BGP the most abundant component were the proteins (up tp 45 %), which makes this alga an excellent unconventional protein producer. As opposed to it, the biochemical composition of Porphyridium cruentum was dominated by carbohydrates (up to 57%), but there was also a high content of some essential polyunsaturated fatty acids: arachidonic (AA, 20:4; 13-29%) and eicosapentaenoic acid (EPA, 20:5, 24-25%). CONCLUSIONS: Microalgae are a really remarkable source of biomass and a wide range of substances, but this area is poorly explored. Both of the microalgal strains proved to be important sources of functional ingredients that could be successfully used as food additives together or separately.
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9

Bocanegra, Aránzazu, Adrián Macho-González, Alba Garcimartín, Juana Benedí, and Francisco José Sánchez-Muniz. "Whole Alga, Algal Extracts, and Compounds as Ingredients of Functional Foods: Composition and Action Mechanism Relationships in the Prevention and Treatment of Type-2 Diabetes Mellitus." International Journal of Molecular Sciences 22, no. 8 (April 7, 2021): 3816. http://dx.doi.org/10.3390/ijms22083816.

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Анотація:
Type-2 diabetes mellitus (T2DM) is a major systemic disease which involves impaired pancreatic function and currently affects half a billion people worldwide. Diet is considered the cornerstone to reduce incidence and prevalence of this disease. Algae contains fiber, polyphenols, ω-3 PUFAs, and bioactive molecules with potential antidiabetic activity. This review delves into the applications of algae and their components in T2DM, as well as to ascertain the mechanism involved (e.g., glucose absorption, lipids metabolism, antioxidant properties, etc.). PubMed, and Google Scholar databases were used. Papers in which whole alga, algal extracts, or their isolated compounds were studied in in vitro conditions, T2DM experimental models, and humans were selected and discussed. This review also focuses on meat matrices or protein concentrate-based products in which different types of alga were included, aimed to modulate carbohydrate digestion and absorption, blood glucose, gastrointestinal neurohormones secretion, glycosylation products, and insulin resistance. As microbiota dysbiosis in T2DM and metabolic alterations in different organs are related, the review also delves on the effects of several bioactive algal compounds on the colon/microbiota-liver-pancreas-brain axis. As the responses to therapeutic diets vary dramatically among individuals due to genetic components, it seems a priority to identify major gene polymorphisms affecting potential positive effects of algal compounds on T2DM treatment.
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10

Makwana, Hiren V., Priyanka G. Pandey, and Binita A. Desai. "Phytochemical Analysis and Evaluation of Total Phenolic Content of Algal Biomass Found in Tapi River in Surat." International Journal for Research in Applied Science and Engineering Technology 10, no. 4 (April 30, 2022): 2783–87. http://dx.doi.org/10.22214/ijraset.2022.41897.

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Анотація:
Abstract: The present study aims to assess the phytochemicals of algal biomass which has seven species identified microscopically. The qualitative phytochemical analysis was done on all the biomass in the Tapi River, Surat in Gujarat. For the qualitative phytochemical analysis total 12 different parameters were analysed and used on algal biomass. Extract prepared in water. Amongst the water extract showed the presence of a maximum number of phytochemical compounds. Next to that, water extract showed alkaloids, glycosides, flavonoids, saponins, terpenoids, phytosterol, coumarins, tannins, diterpenes, and quinones, carbohydrates, The protein present in algal biomass. The evaluation of total phenolic content presence in algal biomass. Keywords: Algal biomass, Phytochemical analysis, Algae, Phytochemicals, Total phenol content, Qualitative analysis of biomass, Tapi, Biomass Extract, Algae extract.
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Більше джерел

Дисертації з теми "Algal Protein"

1

Turkina, Maria. "Functional proteomics of protein phosphorylation in algal photosynthetic membranes." Doctoral thesis, Linköping : Univ, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-10708.

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2

Bosley, Amber L. "Algae Characterization and Processing Techniques." University of Toledo / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1321538296.

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3

Casey, Diane M. "DC3, a Calcium-Binding Protein Important for Assembly of the Chlamydomonas Outer Dynein Arm: a Dissertation." eScholarship@UMMS, 2005. http://escholarship.umassmed.edu/gsbs_diss/156.

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The outer dynein arm-docking complex (ODA-DC) specifies the outer dynein arm-binding site on the flagellar axoneme. The ODA-DC of Chlamydomonas contains equimolar amounts of three proteins termed DC1, DC2, and DC3 (Takada et al., 2002). DC1 and DC2 are predicted to be coiled-coil proteins, and are encoded by ODA3 and ODA1, respectively (Koutoulis et al., 1997; Takada et al., 2002). Prior to this work, nothing was known about DC3. To fully understand the function(s) of the ODA-DC, a detailed analysis of each of its component parts is necessary. To that end, this dissertation describes the characterization of the smallest subunit, DC3. In Chapter II, I report the isolation and sequencing of genomic and full-length cDNA clones encoding DC3. The sequence predicts a 21,341 D protein with four EF hands that is a member of the CTER (Calmodulin, Troponin C, Essential and Regulatory myosin light chains) group and is most closely related to a predicted protein from Plasmodium. The DC3 gene, termed ODA14, is intronless. Chlamydomonas mutants that lack DC3 exhibit slow, jerky swimming due to loss of some but not all, outer dynein arms. Some outer doublet microtubules without arms had a "partial" docking complex, indicating that DC1 and DC2 can assemble in the absence of DC3. In contrast, DC3 cannot assemble in the absence of DC1 or DC2. Transformation of a DC3-deletion strain with the wild-type DC3 gene rescued both the motility phenotype and the structural defect, whereas a mutated DC3 gene was incompetent to rescue. The results indicate that DC3 is important for both outer arm and ODA-DC assembly. As mentioned above, DC3 has four EF-hands: two fit the consensus pattern for calcium binding and one contains two cysteine residues within its binding loop. To determine if the consensus EF-hands are functional, I purified bacterially expressed wild-type DC3 and analyzed its calcium-binding potential in the presence and absence of DTT and Mg2+. As reported in Chapter III, the protein bound one calcium ion with an affinity (Kd) of ~1 x 10-5 M. Calcium binding was observed only in the presence of DTT and thus is redox sensitive. DC3 also bound Mg2+ at physiological concentrations, but with a much lower affinity. Changing the essential glutamate to glutamine in both EF-hands eliminated the calcium-binding activity of the bacterially expressed protein. To investigate the role of the EF hands in vivo, I transformed the modified DC3 gene into a Chlamydomonas insertional mutant lacking DC3. The transformed strain swam normally, assembled a normal number of outer arms, and had a normal photoshock response, indicating that the E to Q mutations did not affect ODA-DC assembly, outer arm assembly, or Ca2+-mediated outer arm activity. Thus, DC3 is a true calcium-binding protein, but the function of this activity remains obscure. In Chapter IV, I report the initial characterization of a DC3 insertional mutant having a phenotype intermediate between that of the DC3-deletion strain and wild type. Furthermore, I suggest future experiments that may help elucidate the specific role of DC3 in outer arm assembly and ODA-DC function. Lastly, I speculate that the ODA-DC may play a role in flagellar regeneration.
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4

Casey, Diane M. "DC3, a Calcium-Binding Protein Important for Assembly of the Chlamydomonas Outer Dynein Arm: a Dissertation." eScholarship@UMMS, 2003. https://escholarship.umassmed.edu/gsbs_diss/156.

Повний текст джерела
Анотація:
The outer dynein arm-docking complex (ODA-DC) specifies the outer dynein arm-binding site on the flagellar axoneme. The ODA-DC of Chlamydomonas contains equimolar amounts of three proteins termed DC1, DC2, and DC3 (Takada et al., 2002). DC1 and DC2 are predicted to be coiled-coil proteins, and are encoded by ODA3 and ODA1, respectively (Koutoulis et al., 1997; Takada et al., 2002). Prior to this work, nothing was known about DC3. To fully understand the function(s) of the ODA-DC, a detailed analysis of each of its component parts is necessary. To that end, this dissertation describes the characterization of the smallest subunit, DC3. In Chapter II, I report the isolation and sequencing of genomic and full-length cDNA clones encoding DC3. The sequence predicts a 21,341 D protein with four EF hands that is a member of the CTER (Calmodulin, Troponin C, Essential and Regulatory myosin light chains) group and is most closely related to a predicted protein from Plasmodium. The DC3 gene, termed ODA14, is intronless. Chlamydomonas mutants that lack DC3 exhibit slow, jerky swimming due to loss of some but not all, outer dynein arms. Some outer doublet microtubules without arms had a "partial" docking complex, indicating that DC1 and DC2 can assemble in the absence of DC3. In contrast, DC3 cannot assemble in the absence of DC1 or DC2. Transformation of a DC3-deletion strain with the wild-type DC3 gene rescued both the motility phenotype and the structural defect, whereas a mutated DC3 gene was incompetent to rescue. The results indicate that DC3 is important for both outer arm and ODA-DC assembly. As mentioned above, DC3 has four EF-hands: two fit the consensus pattern for calcium binding and one contains two cysteine residues within its binding loop. To determine if the consensus EF-hands are functional, I purified bacterially expressed wild-type DC3 and analyzed its calcium-binding potential in the presence and absence of DTT and Mg2+. As reported in Chapter III, the protein bound one calcium ion with an affinity (Kd) of ~1 x 10-5 M. Calcium binding was observed only in the presence of DTT and thus is redox sensitive. DC3 also bound Mg2+ at physiological concentrations, but with a much lower affinity. Changing the essential glutamate to glutamine in both EF-hands eliminated the calcium-binding activity of the bacterially expressed protein. To investigate the role of the EF hands in vivo, I transformed the modified DC3 gene into a Chlamydomonas insertional mutant lacking DC3. The transformed strain swam normally, assembled a normal number of outer arms, and had a normal photoshock response, indicating that the E to Q mutations did not affect ODA-DC assembly, outer arm assembly, or Ca2+-mediated outer arm activity. Thus, DC3 is a true calcium-binding protein, but the function of this activity remains obscure. In Chapter IV, I report the initial characterization of a DC3 insertional mutant having a phenotype intermediate between that of the DC3-deletion strain and wild type. Furthermore, I suggest future experiments that may help elucidate the specific role of DC3 in outer arm assembly and ODA-DC function. Lastly, I speculate that the ODA-DC may play a role in flagellar regeneration.
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5

Ronzitti, Giuseppe <1979&gt. "Le tossine algali alterano proteine dell'adesione cellulare." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2008. http://amsdottorato.unibo.it/644/1/Tesi_Ronzitti_Giuseppe.pdf.

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6

Ronzitti, Giuseppe <1979&gt. "Le tossine algali alterano proteine dell'adesione cellulare." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2008. http://amsdottorato.unibo.it/644/.

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7

Borgen, Kelly. "Evaluation of physicochemical properties of modified algae protein adhesives." Thesis, Kansas State University, 2012. http://hdl.handle.net/2097/13634.

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Анотація:
Master of Science
Department of Biological and Agricultural Engineering
Donghai Wang
Algae proteins have similar amino acid compositions as conventional plant proteins, and are comparatively richer in the essential amino acids. Algae protein has the potential to be used in the development of a wide variety of products, including foods, animal feeds, bioplastics, and adhesives. The utilization of algae protein for value-added products would increase the economic feasibility of algae biodiesel. This research evaluated the adhesion, rheological, morphological, and thermal properties of adhesives made from algae protein extracted from Cladophora sp. and modified with either sodium hydroxide (pH 9, 10, 11) or sodium dodecyl sulfate (SDS, 0.5, 1, and 3%). Both alkali-modified and SDS-modified algae protein adhesives displayed improved dry shear strength compared to unmodified algae protein. However, only 3% SDS-modified algae protein significantly improved the water resistance as shown in wet and soak shear strength tests. Thermal analysis using differential scanning calorimetry showed that SDS modification caused complete denaturation of the algae protein. SDS modification also increased the viscosity of the adhesive and created rougher particle surface texture. These data suggest that SDS modification can effectively increase shear strength and water resistance of algae protein adhesives caused by protein denaturation and protein structure change.
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8

Azevedo, Brian. "Algae as an economical protein source for dairy cattle nutrition." Click here to view, 2009. http://digitalcommons.calpoly.edu/dscisp/23/.

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Анотація:
Thesis (B.S.)--California Polytechnic State University, 2009.
Project advisor: Edwin H. Jaster. Title from PDF title page; viewed on Jan. 28, 2010. Includes bibliographical references. Also available on microfiche.
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9

Djabayan-Djibeyan, Pablo. "A comparison of lectins in green Venezuelan marine algae." Thesis, University of Portsmouth, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.343338.

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10

Lupatini, Anne Luize. "Extração de proteínas e carboidratos da biomassa de Spirulina platensis e caracterização da fração proteica." Universidade Tecnológica Federal do Paraná, 2016. http://repositorio.utfpr.edu.br/jspui/handle/1/2180.

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CAPES; CNPQ
A Spirulina platensis é reconhecida como uma fonte não convencional de proteínas, em função da sua constituição favorável deste nutriente (46 a 63%), possuindo concentração superior a das carnes e da soja. Além disso, apresenta potencial como matéria-prima para a produção de bioetanol, podendo acumular entre 8,0 e 14,0% de carboidratos. A fim de abranger o conceito de Biorrefinarias Integradas, o objetivo deste trabalho consistiu em avaliar a extração conjunta de proteínas e carboidratos da biomassa de Spirulina platensis utilizando tratamento ultrassônico e agitação em meio alcalino, e a posterior produção e caracterização do concentrado proteico. Na primeira etapa do trabalho, aplicou-se uma estratégia sequencial de planejamento experimental (Planejamento Fatorial Fracionário (PFF) seguido de Delineamentos Compostos Centrais Rotacionais (DCCR)) para seleção e maximização das variáveis com influência significativa sobre o processo de extração. Com as condições de extração otimizadas, foi possível atingir recuperação final de 75,85% e de 41,54% de proteínas e carboidratos, respectivamente. Na segunda etapa do trabalho foi realizada a precipitação de proteínas, para a separação da fase líquida contendo os carboidratos e obtenção do concentrado proteico, o qual foi caracterizado quimicamente e de acordo com sua funcionalidade tecnológica. O concentrado proteico apresentou coloração verde azulada com 75,97% de proteínas (b.s.), concentrações apreciáveis de aminoácidos, sendo o que o triptofano apresentou o maior escore químico (1,71) e o aminoácido limitante foi a histidina; na análise da estrutura secundária das proteínas, as conformações mais abundantes foram β-folha e α-hélice. Na etapa de avaliação da funcionalidade tecnológica observou-se que o pH apresentou influência nas propriedades de capacidade de absorção de água, capacidade de formação e estabilidade de espuma e emulsão, e capacidade de formação de gel, o que pode ser justificado pela solubilidade desta proteína, que é mínima em pH 3,0 e máxima em 9,0. A concentração de concentrado proteico também interferiu no desempenho destas propriedades; melhores resultados foram obtidos em maiores níveis de concentração, exceto para a capacidade de absorção de água e de óleo. Desta forma foi possível determinar que as proteínas de Spirulina platensis podem contribuir na formulação de alimentos, possuindo características eficazes de formação de emulsões, espumas ou géis, bem como pode ser utilizada como fonte suplementar de proteínas.
Spirulina platensis is considered an unconventional source of protein, because its avorably constitution on this component (46 to 63%), which is higher than the meat and soy. Furthermore, it has potential as a feedstock for bioethanol production and can accumulate between 8.0 to 14.0% of carbohydrate. In order to cover the concept of Integrated Biorefineries, the aim of this study was to evaluate the combined extraction of proteins and carbohydrates from Spirulina platensis biomass using sonication and agitation, under alkaline conditions, and the subsequent production and characterization of protein concentrate. The first stage of this work consisted of applying a sequential strategy of experimental design (Fractional Factorial Design FFD) and Central Composite Rotatable Design (CCRD)) by selecting and maximizing variables with significant influence on the protein and carbohydrates extraction. With the extraction conditions established, a final yield of 75.85% and 41.54% from protein and carbohydrate, respectively, was reached. In the second step, the protein concentrate obtained by precipitation was submitted to chemical and echnological functionality analyzes. The protein concentrate showed blue-green color with 75.97% of proteins (dry weight), appreciable concentrations of amino acids, where tryptophan had the highest chemical score (1.71) and the limiting amino acid was histidine; the secondary structure of proteins showed that the most abundant conformations present were β-sheet and α-helice. At the step of echnological functionality evaluation it was observed that the pH influenced on the properties of water absorption capacity, foaming and emulsion capacity and stability, and gelation capacity; it can be justified by the solubility of this protein which is minimal at pH 3.0 and maximum at 9.0. The level of addition of protein concentrate also interfered on the performance of these properties; better results have been obtained at higher concentrations levels, except for water and oil absorption capacity. Thus, it was confirmed that the Spirulina platensis proteins may contribute in different ormulations of foods, having effective characteristics to form emulsions, foams or gels, and can be used as a supplemental source of protein.
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Книги з теми "Algal Protein"

1

Lohrenz, Steven E. Primary production of particulate protien amino acids: Algal protein metabolism and its relationship to the composition of particulate organic matter. Woods Hole, Mass: Woods Hole Oceanographic Institution, 1985.

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Karel, Marcus. Utilization of non-conventional systems for conversion of biomass to food components: Potential for utilization of algae in engineered foods. Cambridge, MA: Dept. of Applied Biological Sciences, Massachusetts Institute of Technology, 1985.

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3

Karel, Marcus. Utilization of non-conventional systems for conversion of biomass to food components: Final report. Cambridge, MA: Dept. of Chemical Engineering, Massachusetts Institute of Technology, 1989.

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4

United States. National Aeronautics and Space Administration, ed. Utilization of non-conventional systems for conversion of biomass to food components: Potential for utilization of algae in engineered foods. Cambridge, MA: Dept. of Applied Biological Sciences, Massachusetts Institute of Technology, 1985.

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5

Karel, Marcus. Utilization of non-conventional systems for conversion of biomass to food components: Potential for utilization of algae in engineered foods. Cambridge, MA: Dept. of Applied Biological Sciences, Massachusetts Institute of Technology, 1985.

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6

United States. National Aeronautics and Space Administration., ed. Utilization of non-conventional systems for conversion of biomass to food components: Potential for utilization of algae in engineered foods. Cambridge, MA: Dept. of Applied Biological Sciences, Massachusetts Institute of Technology, 1985.

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7

Karel, Marcus. Utilization of non-conventional systems for conversion of biomass to food components: Recovery optimization and characterization of algal proteins and lipids ; status report (March 1985 to June 1986). Cambridge, MA: Dept. of Applied Biological Sciences, Massachusetts Institute of Technology, 1986.

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8

Z, Nakhost, and United States. National Aeronautics and Space Administration, eds. Utilization of non-conventional systems for conversion of biomass to food components: Recovery optimization and characterization of algal proteins and lipids ; status report (March 1985 to June 1986). Cambridge, MA: Dept. of Applied Biological Sciences, Massachusetts Institute of Technology, 1986.

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9

United States. National Aeronautics and Space Administration, ed. Utilization of non-conventional systems for conversion of biomass to food components: Final report. Cambridge, MA: Dept. of Chemical Engineering, Massachusetts Institute of Technology, 1989.

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10

United States. National Aeronautics and Space Administration., ed. Utilization of non-conventional systems for conversion of biomass to food components: Final report. Cambridge, MA: Dept. of Chemical Engineering, Massachusetts Institute of Technology, 1989.

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Частини книг з теми "Algal Protein"

1

Richard, Hamilton, Nizovtseva Irina, Chernuskin Dmitri, and Marina G. Kalyuzhnaya. "C1-Proteins Prospect for Production of Industrial Proteins and Protein-Based Materials from Methane." In Algal Biorefineries and the Circular Bioeconomy, 251–76. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003195429-7.

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2

Rojo, Elena M., Alejandro Filipigh, David Moldes, Marisol Vega, and Silvia Bolado. "Potential of Microalgae for Protein Production." In Algal Biorefineries and the Circular Bioeconomy, 91–132. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003195405-4.

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3

Knoshaug, Eric P., Alida T. Gerritsen, Calvin A. Henard, and Michael T. Guarnieri. "Methods for Algal Protein Isolation and Proteome Analysis." In Methods in Molecular Biology, 51–59. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0195-2_5.

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4

Nigam, Mohit, Ruchi Yadav, and Garima Awasthi. "In-Silico Construction of Hybrid ORF Protein to Enhance Algal Oil Content for Biofuel." In Advances in Biomedical Engineering and Technology, 67–89. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-6329-4_8.

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5

Yucetepe, Aysun. "Strategies for Nanoencapsulation of Algal Proteins, Protein Hydrolysates and Bioactive Peptides: The Effect of Encapsulation Techniques on Bioactive Properties." In Nanotechnology in the Life Sciences, 211–27. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-81557-8_9.

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Chirdon, William M. "Utilization of Biorefinery Waste Proteins as Feed, Glues, Composites, and Other Co-Products." In Algal Biorefineries, 367–92. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-20200-6_11.

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7

Irvani, Neda, Alan Carne, Dominic Agyei, and Indrawati Oey. "Algae as an Alternative Source of Protein." In Alternative Proteins, 65–84. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9780429299834-4.

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8

Olatunji, Ololade. "Aquatic Plants and Algae Proteins." In Springer Series on Polymer and Composite Materials, 211–32. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-34709-3_10.

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9

Jeon, You-Jin, and Kalpa Samarakoon. "Recovery of Proteins and their Biofunctionalities from Marine Algae." In Marine Proteins and Peptides, 253–69. Chichester, UK: John Wiley & Sons, Ltd, 2013. http://dx.doi.org/10.1002/9781118375082.ch12.

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Grimme, L. H., I. Damm, D. Steinmetz, and B. Scheffczyk. "Pigment-Protein Complexes of Algal Thylakoid Membranes: Variations in Pattern, Pigment Composition and Reaction Centre II Types During The Cell Cycle of Chlorella Fusca and after Adaptation to Low Light Intensities." In Progress in Photosynthesis Research, 347–50. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3535-8_84.

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Тези доповідей конференцій з теми "Algal Protein"

1

Quiroz-Arita, Carlos. "High-protein algal bioproducts: An economic and environmental sustainability review and risk analysis." In Proposed for presentation at the International Conference on Algal Biomass, Biofuels & Bioproducts held June 14-16, 2021, Virtual, United States. US DOE, 2021. http://dx.doi.org/10.2172/1873059.

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2

Freeman, Eric, Lisa Mauck Weiland, and Ryan Soncini. "Water Purification Through Selective Transport." In ASME 2011 Conference on Smart Materials, Adaptive Structures and Intelligent Systems. ASMEDC, 2011. http://dx.doi.org/10.1115/smasis2011-5062.

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Accumulation of inorganic nitrates and phosphates in regions such as the Mississippi river basin has resulted in catastrophic growth of algal blooms. These algal blooms deplete the surrounding oxygen and asphyxiate nearby aquatic life, resulting in large regions incapable of sustaining life. Using biomimicry principles to design a tailored active material to selectively transport these pollutants may offer a strategy to restore these dead zones to health. Theoretically a combination of selected protein transporters may be employed to create a selective sponge to reclaim these nitrates and phosphates. Presented is a feasibility study of various configurations of transporters, and a unique solution for restoring the aquatic ecosystem back to health.
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3

Calinescu, Ioan, Alin Vintila, Aurel Diacon, Mircea Vinatoru, Ana Maria Galan, and Sanda Velea. "GROWTH OF NANNOCHLORIS ALGAE IN THE PRESENCE OF MICROWAVES (CONTINUOUS REACTOR)." In Ampere 2019. Valencia: Universitat Politècnica de València, 2019. http://dx.doi.org/10.4995/ampere2019.2019.9820.

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Algae are very effective in capturing the sun's energy, carbon dioxide from the atmosphere, and nutrients to turn them into useful substances (carbohydrates, oils, proteins, etc.). Besides the main metabolites, there are also secondary metabolites, such as carotenoids (astaxanthin, β-carotene, lutein, lycopene, and canthaxanthin [1]). Both major and compounds existing in small amounts in algae are useful. Oils and carbohydrates could provide biofuels, proteins can get products with nutritional value and from carotenoids can be prepared food supplements. Obtaining biofuel from algae has not yet proved to be economically viable [2, 3]. A much higher interest might be getting food supplements from algae. To increase their value as ingredients for food supplements, algal oils should have a higher degree of unsaturation (rich in omega 3) and an increased carotenoid content to be an important additional benefit in over all processing of algae. There are studies that refer to the influence of environmental factors on algae composition [2], but the microwave influence on algae growth, especially algal metabolites composition change is very poor studied. In this paper, besides the experiments for the activation of algal growth in discontinuous reactors [4] additional work was conducted in a continuous photobioreactor. The goal was checking not only the growth of microalgae but also their content in polyunsaturated oil and in carotenoids. By microwave-controlled irradiation of the nutrient and algae flux, which is recirculated through the photobioreactor and through a glass reactor located in a TE-type monomod cavity, the lipid content of the algae increased, but only, the modification of the lipid fraction content was significantly increased in the concentration of polyunsaturated acids with 16 and 18 carbon atoms. As far as carotenoids are concerned, the algae nannochloris has a higher carotenoid content over many known vegetables holding carotene or lycopene (carrots or tomatoes). Besides oil increasing microwave treatment produced a significant increase in carotenoid content of algae. They can be extracted together with omega-3-rich algal oil and are the basis of very valuable dietary supplements.
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4

Bounnit, Touria, Imen Saadaoui, Rihab Rasheed, Hareb Al jabri, Sami Sayadi, and Ahmad Ayesh. "Assessment of SnO2 Nanoparticles’ Impact on local Pichoclorum Atomus Growth Performance, Cell Morphology and Metabolites Content." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2021. http://dx.doi.org/10.29117/quarfe.2021.0034.

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Oxide nanoparticles are among the most used nanomaterials and have received considerable attention over their potential ecological effects. Increasing investigations report toxicity of certain oxide nanoparticles, however, there are also studies showing opposite results, highlighting the fact that these nanoparticles may differ in their toxicological effects, which depend on particle variety and size, test organism species, and test method. The current study investigates the ecotoxicity of SnO2 nanoparticles on a local marine algae isolate. Five different concentrations (1, 5, 25, 50 and 100mg/L) were tested and the culture was followed for 72h. Algae growth, morphology and metabolites were followed each 24h. The obtained data showed that the SnO2 presented a toxicity on the algae growth that was decreasing with the dose, with lower doses presenting more negative impacts than the higher doses. In parallel, the slow growth observed at 1-5 mg/L was explained by the dramatic damages caused by the SnO2 on the cell morphology, which was detected using the scanning electronic microscopy. Indeed, this low negative impact of higher concentrations of SnO2 (50-100mg/L) is explained by the high agglomeration of ten particles leading to reduced effect on the cell morphology and health. Furthermore, and in accordance with the morphological data, the metabolites analysis data revealed that SnO2 nanoparticles induced stress, which was manifested by an increase in the lipid content and decrease in the proteins, a metabolite that is involved in the algal growth.
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5

B.C., Meskhi, Mozgovoy A.V., Rudoy D.V., Olshevskaya A.V., Smirnova O.A., Sarkisian D.S., and Maltseva T.A. "ALTERNATIVE SOURCES OF PROTEIN AS A RAW MATERIAL FOR THE PRODUCTION OF NEW FOOD PRODUCTS: PROBLEMS AND PROSPECTS." In OF THE ANNIVERSARY Х INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE «INNOVATIVE TECHNOLOGIES IN SCIENCE AND EDUCATION» («ITSE 2022» CONFERENCE). DSTU-Print, 2022. http://dx.doi.org/10.23947/itse.2022.160-166.

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The article discusses the problems and prospects of using non-traditional raw materials as an alternative source of protein in food production. The main types of raw materials that contain a large amount of protein and can serve as a substitute for the classic food protein are given: insects, vegetable raw materials, cultured meat, algae and synthetic amino acids. The methods of obtaining them are considered, the advantages and disadvantages are revealed.
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6

Hobbs, Raymond, and Xiaolei Sun. "Integrated Wind, Sun, Fossil, Biomass and Nuclear for Energy Sustainability." In ASME 2009 3rd International Conference on Energy Sustainability collocated with the Heat Transfer and InterPACK09 Conferences. ASMEDC, 2009. http://dx.doi.org/10.1115/es2009-90129.

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The Advanced Hydrogasification Process (AHP) is being developed at Arizona Public Service (APS) to utilize the America’s abundant western coal supply to address concerns of diminishing domestic oil and natural gas resources as energy providers, while also incorporating a renewable energy and reducing greenhouse gas emissions. The AHP utilizes coal as a source for carbon, and hydrogen produced by renewable energy in the hydrogasification process to produce substitute natural gas (SNG) that can be fed to existing natural gas pipeline. The hydrogen will be produced through water electrolysis using wind firmed with off peak nuclear/coal electricity. The CO2 produced from the process will be recycled through biological approach — algae farming. With water and sun, algae will convert CO2 into starch, protein and lipids by photosynthesis.
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7

Beaulieu, Lucie. "Algae: A Key Protein Source for the Development of New Functional Ingredients." In Virtual 2021 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2021. http://dx.doi.org/10.21748/am21.150.

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Natsir, Hasnah, Marinda, Ahyar Ahmad, Abdul Wahid Wahab, Nunuk Hariani Soekamto, Siti Fauziah, Yusriadi, Rafsanjany Ramadan, Harningsih Karim, and Fatahu. "Hydrolisis enzymatic protein from microsimbiont red algae Eucheuma cottonii as an antibacterial." In INTERNATIONAL CONFERENCE ON ENERGY AND ENVIRONMENT (ICEE 2021). AIP Publishing, 2021. http://dx.doi.org/10.1063/5.0059610.

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Sethi, Purnima, Mohit Prasad, and Sukhdev Roy. "All-optical switching in LOV2-C250S protein mutant from Chlamydomonas reinhardtii green algae." In 2009 International Conference on Emerging Trends in Electronic and Photonic Devices & Systems (ELECTRO-2009). IEEE, 2009. http://dx.doi.org/10.1109/electro.2009.5441039.

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10

Khairunnur, Siti, Ahyar Ahmad, Rugaiyah A. Arfah, Firdaus Zenta, Nursiah La Nafie, Hasnah Natsir, and Andi Akbar. "Isolation and identification of anticancer-protein-producing symbiotic bacteria from green algae Caulerpa lentillifera." In THE 9TH INTERNATIONAL CONFERENCE OF THE INDONESIAN CHEMICAL SOCIETY ICICS 2021: Toward a Meaningful Society. AIP Publishing, 2022. http://dx.doi.org/10.1063/5.0104089.

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Звіти організацій з теми "Algal Protein"

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Mitchell, Brian G., Amir Neori, Charles Yarish, D. Allen Davis, Tzachi Samocha, and Lior Guttman. The use of aquaculture effluents in spray culture for the production of high protein macroalgae for shrimp aqua-feeds. United States Department of Agriculture, January 2013. http://dx.doi.org/10.32747/2013.7597934.bard.

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The FAO has projected a doubling in world demand for seafood during the 21 ed from aquaculture of marine fish and shrimps fed primarily on fishmeal-based aquafeeds. However, current practices of high intensity monoculture of shrimp in coastal ponds and fish in offshore pens have been strongly criticized as being ecologically and socially unsustainable. This view derives from un- checked eutrophication of coastal marine ecosystems from fish farm effluents, and the destruction of coastal estuarine ecosystems by shrimp farm constructions, plus aquaculture’s reliance on wild-caught small fish - which are excellent food for humans, but instead are rendered into fishmeal and fish oil for formulating aquafeeds. Fishmeal-sparing and waste- reduction aquafeeds can only delay the time when fed aquaculture product are priced out of affordability for most consumers. Additionally, replacement of fishmeal protein and fish oil by terrestrial plant sources such as soybean meal and oil directly raises food costs for human communities in developing nations. New formulations incorporating sustainably-produced marine algal proteins and oils are growing in acceptance as viable and practical alternatives. This BARD collaborative research project investigated a sustainable water-sparing spray/drip culture method for producing high-protein marine macrophyte meals for incorporation into marine shrimp and fish diets. The spray culture work was conducted at laboratory-scale in the USA (UCSD-SIO) using selected Gracilariaand Ulvastrains isolated and supplied by UCONN, and outdoors at pilot-scale in Israel (IOLR-NCM) using local strains of Ulvasp., and nitrogen/phosphorus-enriched fish farm effluent to fertilize the spray cultures and produce seaweed biomass and meals containing up to 27% raw protein (dry weight content). Auburn University (USA) in consultation with TAMUS (USA) used the IOLR meals to formulate diets and conduct marine shrimp feeding trials, which resulted in mixed outcomes, indicating further work was needed to chemically identify and remove anti-nutritional elements present in the IOLR-produced seaweed meals.
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2

Christopher, David A., and Avihai Danon. Plant Adaptation to Light Stress: Genetic Regulatory Mechanisms. United States Department of Agriculture, May 2004. http://dx.doi.org/10.32747/2004.7586534.bard.

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Original Objectives: 1. Purify and biochemically characterize RB60 orthologs in higher plant chloroplasts; 2. Clone the gene(s) encoding plant RB60 orthologs and determine their structure and expression; 3. Manipulate the expression of RB60; 4. Assay the effects of altered RB60 expression on thylakoid biogenesis and photosynthetic function in plants exposed to different light conditions. In addition, we also examined the gene structure and expression of RB60 orthologs in the non-vascular plant, Physcomitrella patens and cloned the poly(A)-binding protein orthologue (43 kDa RB47-like protein). This protein is believed to a partner that interacts with RB60 to bind to the psbA5' UTR. Thus, to obtain a comprehensive view of RB60 function requires analysis of its biochemical partners such as RB43. Background & Achievements: High levels of sunlight reduce photosynthesis in plants by damaging the photo system II reaction center (PSII) subunits, such as D1 (encoded by the chloroplast tpsbAgene). When the rate of D1 synthesis is less than the rate of photo damage, photo inhibition occurs and plant growth is decreased. Plants use light-activated translation and enhanced psbAmRNA stability to maintain D1 synthesis and replace the photo damaged 01. Despite the importance to photosynthetic capacity, these mechanisms are poorly understood in plants. One intriguing model derived from the algal chloroplast system, Chlamydomonas, implicates the role of three proteins (RB60, RB47, RB38) that bind to the psbAmRNA 5' untranslated leader (5' UTR) in the light to activate translation or enhance mRNA stability. RB60 is the key enzyme, protein D1sulfide isomerase (Pill), that regulates the psbA-RN :Binding proteins (RB's) by way of light-mediated redox potentials generated by the photosystems. However, proteins with these functions have not been described from higher plants. We provided compelling evidence for the existence of RB60, RB47 and RB38 orthologs in the vascular plant, Arabidopsis. Using gel mobility shift, Rnase protection and UV-crosslinking assays, we have shown that a dithiol redox mechanism which resembles a Pill (RB60) activity regulates the interaction of 43- and 30-kDa proteins with a thermolabile stem-loop in the 5' UTR of the psbAmRNA from Arabidopsis. We discovered, in Arabidopsis, the PD1 gene family consists of II members that differ in polypeptide length from 361 to 566 amino acids, presence of signal peptides, KDEL motifs, and the number and positions of thioredoxin domains. PD1's catalyze the reversible formation an disomerization of disulfide bonds necessary for the proper folding, assembly, activity, and secretion of numerous enzymes and structural proteins. PD1's have also evolved novel cellular redox functions, as single enzymes and as subunits of protein complexes in organelles. We provide evidence that at least one Pill is localized to the chloroplast. We have used PDI-specific polyclonal and monoclonal antisera to characterize the PD1 (55 kDa) in the chloroplast that is unevenly distributed between the stroma and pellet (containing membranes, DNA, polysomes, starch), being three-fold more abundant in the pellet phase. PD1-55 levels increase with light intensity and it assembles into a high molecular weight complex of ~230 kDa as determined on native blue gels. In vitro translation of all 11 different Pill's followed by microsomal membrane processing reactions were used to differentiate among PD1's localized in the endoplasmic reticulum or other organelles. These results will provide.1e insights into redox regulatory mechanisms involved in adaptation of the photosynthetic apparatus to light stress. Elucidating the genetic mechanisms and factors regulating chloroplast photosynthetic genes is important for developing strategies to improve photosynthetic efficiency, crop productivity and adaptation to high light environments.
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Nango, Mamoru. Assembly of Photosynthetic Antenna Protein Complexes from Algae for Development of Nano-biodevice and Its Fuelization. Fort Belvoir, VA: Defense Technical Information Center, May 2013. http://dx.doi.org/10.21236/ada586787.

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Vakharia, Vikram, Shoshana Arad, Yonathan Zohar, Yacob Weinstein, Shamila Yusuff, and Arun Ammayappan. Development of Fish Edible Vaccines on the Yeast and Redmicroalgae Platforms. United States Department of Agriculture, February 2013. http://dx.doi.org/10.32747/2013.7699839.bard.

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Betanodaviruses are causative agents of viral nervous necrosis (VNN), a devastating disease of cultured marine fish worldwide. Betanodavirus (BTN) genome is composed of two single-stranded, positive-sense RNA molecules. The larger genomic segment, RNA1 (3.1 kb), encodes the RNA-dependent RNA polymerase, while the smaller genomic segment, RNA 2 (1.4kb), encodes the coat protein. This structural protein is the host-protective antigen of VNN which assembles to form virus-like particles (VLPs). BTNs are classified into four genotypes, designated red-spotted grouper nervous necrosis virus (RGNNV), barfin flounder nervous necrosis virus (BFNNV), tiger puffer nervous necrosis virus (TPNNV), and striped jack nervous necrosis virus (SJNNV), based on phylogenetic analysis of the coat protein sequences. RGNNV type is quite important as it has a broad host-range, infecting warm-water fish species. At present, there is no commercial vaccine available to prevent VNN in fish. The general goal of this research was to develop oral fish vaccines in yeast and red microalgae (Porphyridium sp.) against the RGNNV genotype. To achieve this, we planned to clone and sequence the coat protein gene of RGNNV, express the coat protein gene of RGNNV in yeast and red microalgae and evaluate the immune response in fish fed with recombinantVLPs antigens produced in yeast and algae. The collaboration between the Israeli group and the US group, having wide experience in red microalgae biochemistry, molecular genetics and large-scale cultivation, and the development of viral vaccines and eukaryotic protein expression systems, respectively, was synergistic to produce a vaccine for fish that would be cost-effective and efficacious against the betanodavirus infection.
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Ohad, Itzhak, and Himadri Pakrasi. Role of Cytochrome B559 in Photoinhibition. United States Department of Agriculture, December 1995. http://dx.doi.org/10.32747/1995.7613031.bard.

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The aim of this research project was to obtain information on the role of the cytochrome b559 in the function of Photosystem-II (PSII) with special emphasis on the light induced photo inactivation of PSII and turnover of the photochemical reaction center II protein subunit RCII-D1. The major goals of this project were: 1) Isolation and sequencing of the Chlamydomonas chloroplast psbE and psbF genes encoding the cytochrome b559 a and b subunits respectively; 2) Generation of site directed mutants and testing the effect of such mutation on the function of PSII under various light conditions; 3) To obtain further information on the mechanism of the light induced degradation and replacement of the PSII core proteins. This information shall serve as a basis for the understanding of the role of the cytochrome b559 in the process of photoinhibition and recovery of photosynthetic activity as well as during low light induced turnover of the D1 protein. Unlike in other organisms in which the psbE and psbF genes encoding the a and b subunits of cytochrome b559, are part of an operon which also includes the psbL and psbJ genes, in Chlamydomonas these genes are transcribed from different regions of the chloroplast chromosome. The charge distribution of the derived amino-acid sequences of psbE and psbF gene products differs from that of the corresponding genes in other organisms as far as the rule of "positive charge in" is concerned relative to the process of the polypeptide insertion in the thylakoid membrane. However, the sum of the charges of both subunits corresponds to the above rule possibly indicating co-insertion of both subunits in the process of cytochrome b559 assembly. A plasmid designed for the introduction of site-specific mutations into the psbF gene of C. reinhardtii. was constructed. The vector consists of a DNA fragment from the chromosome of C. reinhardtii which spans the region of the psbF gene, upstream of which the spectinomycin-resistance-conferring aadA cassette was inserted. This vector was successfully used to transform wild type C. reinhardtii cells. The spectinomycin resistant strain thus obtained can grow autotrophically and does not show significant changes as compared to the wild-type strain in PSII activity. The following mutations have been introduced in the psbF gene: H23M; H23Y; W19L and W19. The replacement of H23 involved in the heme binding to M and Y was meant to permit heme binding but eventually alter some or all of the electron transport properties of the mutated cytochrome. Tryptophane W19, a strictly conserved residue, is proximal to the heme and may interact with the tetrapyrole ring. Therefore its replacement may effect the heme properties. A change to tyrosine may have a lesser affect on the potential or electron transfer rate while a replacement of W19 by leucine is meant to introduce a more prominent disturbance in these parameters. Two of the mutants, FW19L and FH23M have segregated already and are homoplasmic. The rest are still grown under selection conditions until complete segregation will be obtained. All mutants contain assembled and functional PSII exhibiting an increased sensitivity of PSII to the light. Work is still in progress for the detailed characterization of the mutants PSII properties. A tobacco mutant, S6, obtained by Maliga and coworkers harboring the F26S mutation in the b subunit was made available to us and was characterized. Measurements of PSII charge separation and recombination, polypeptide content and electron flow indicates that this mutation indeed results in light sensitivity. Presently further work is in progress in the detailed characterization of the properties of all the above mutants. Information was obtained demonstrating that photoinactivation of PSII in vivo initiates a series of progressive changes in the properties of RCII which result in an irreversible modification of the RCII-D1 protein leading to its degradation and replacement. The cleavage process of the modified RCII-D1 protein is regulated by the occupancy of the QB site of RCII by plastoquinone. Newly synthesized D1 protein is not accumulated in a stable form unless integrated in reassembled RCII. Thus the degradation of the irreversibly modified RCII-D1 protein is essential for the recovery process. The light induced degradation of the RCII-D1 protein is rapid in mutants lacking the pD1 processing protease such as in the LF-1 mutant of the unicellular alga Scenedesmus obliquus. In this case the Mn binding site of PSII is abolished, the water oxidation process is inhibited and harmful cation radicals are formed following light induced electron flow in PSII. In such mutants photo-inactivation of PSII is rapid, it is not protected by ligands binding at the QB site and the degradation of the inactivated RCII-D1 occurs rapidly also in the dark. Furthermore the degraded D1 protein can be replaced in the dark in absence of light driven redox controlled reactions. The replacement of the RCII-D1 protein involves the de novo synthesis of the precursor protein, pD1, and its processing at the C-terminus end by an unknown processing protease. In the frame of this work, a gene previously isolated and sequenced by Dr. Pakrasi's group has been identified as encoding the RCII-pD1 C-terminus processing protease in the cyanobacterium Synechocystis sp. PCC 6803. The deduced sequence of the ctpA protein shows significant similarity to the bovine, human and insect interphotoreceptor retinoid-binding proteins. Results obtained using C. reinhardtii cells exposes to low light or series of single turnover light flashes have been also obtained indicating that the process of RCII-D1 protein turnover under non-photoinactivating conditions (low light) may be related to charge recombination in RCII due to back electron flow from the semiquinone QB- to the oxidised S2,3 states of the Mn cluster involved in the water oxidation process.
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Stern, David B., and Gadi Schuster. Manipulation of Gene Expression in the Chloroplast: Control of mRNA Stability and Transcription Termination. United States Department of Agriculture, December 1993. http://dx.doi.org/10.32747/1993.7568750.bard.

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Chloroplasts are the site of photosynthesis and of other essential biosynthetic activities in plant cells. Chloroplasts are semi-autonomous organelles, since they contain their own genomes and protein biosynthetic machinery, but depend on the coordinate expression of nuclear genes to assemble macromolecular complexes. The bioeingineering of plants requires manipulation of chloroplast gene expression, and thus a knowledge of the molecular mechanisms that modulate mRNA and protein production. In this proposal the heterotrophic green alga Chlamydomonas reinhardtii has been used as a model system to understand the control and interrelationships between transcription termination, mRNA 3' end processing and mRNA stability in chloroplasts. Chlamydomonas is a unique and ideal system in which to address these issues, because the chloroplast can be easily manipulated by genetic transformation techniques. This research uncovered new and important information on chloroplast mRNA 3' end formation and mRNA stability. In particular, the 3' untranslated regions of chloroplast mRNAs were shown not to be efficient transcription terminators. The endonucleolytic site in the 3' untranslated region was characterized by site directed mutagensis and the role of several 3' untranslated regions in modulating RNA stability and translation has been studied. This information will allow us to experimentally manipulate the expression of chloroplast genes in vivo by post-transcriptional mechanisms, and should be widely applicable to other higher plant systems.
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Schuster, Gadi, and David Stern. Integrated Studies of Chloroplast Ribonucleases. United States Department of Agriculture, September 2011. http://dx.doi.org/10.32747/2011.7697125.bard.

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Gene regulation at the RNA level encompasses multiple mechanisms in prokaryotes and eukaryotes, including splicing, editing, endo- and exonucleolytic cleavage, and various phenomena related to small or interfering RNAs. Ribonucleases are key players in nearly all of these post-transcriptional mechanisms, as the catalytic agents. This proposal continued BARD-funded research into ribonuclease activities in the chloroplast, where RNase mutation or deficiency can cause metabolic defects and is often associated with plant chlorosis, embryo or seedling lethality, and/or failure to tolerate nutrient stress. The first objective of this proposal was to examined a series of point mutations in the PNPase enzyme of Arabidopsis both in vivo and in vitro. This goal is related to structure-function analysis of an enzyme whose importance in many cellular processes in prokaryotes and eukaryotes has only begun to be uncovered. PNPase substrates are mostly generated by endonucleolytic cleavages for which the catalytic enzymes remain poorly described. The second objective of the proposal was to examine two candidate enzymes, RNase E and RNase J. RNase E is well-described in bacteria but its function in plants was still unknown. We hypothesized it catalyzes endonucleolytic cleavages in both RNA maturation and decay. RNase J was recently discovered in bacteria but like RNase E, its function in plants had yet to be explored. The results of this work are described in the scientific manuscripts attached to this report. We have completed the first objective of characterizing in detail TILLING mutants of PNPase Arabidopsis plants and in parallel introducing the same amino acids changes in the protein and characterize the properties of the modified proteins in vitro. This study defined the roles for both RNase PH core domains in polyadenylation, RNA 3’-end maturation and intron degradation. The results are described in the collaborative scientific manuscript (Germain et al 2011). The second part of the project aimed at the characterization of the two endoribonucleases, RNase E and RNase J, also in this case, in vivo and in vitro. Our results described the limited role of RNase E as compared to the pronounced one of RNase J in the elimination of antisense transcripts in the chloroplast (Schein et al 2008; Sharwood et al 2011). In addition, we characterized polyadenylation in the chloroplast of the green alga Chlamydomonas reinhardtii, and in Arabidopsis (Zimmer et al 2009). Our long term collaboration enabling in vivo and in vitro analysis, capturing the expertise of the two collaborating laboratories, has resulted in a biologically significant correlation of biochemical and in planta results for conserved and indispensable ribonucleases. These new insights into chloroplast gene regulation will ultimately support plant improvement for agriculture.
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NREL Discovers Novel Protein Interaction in Green Algae that Suggests New Strategies to Improve Hydrogen Photoproduction (Fact Sheet). Office of Scientific and Technical Information (OSTI), February 2011. http://dx.doi.org/10.2172/1009293.

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