Добірка наукової літератури з теми "7HF"

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Статті в журналах з теми "7HF"

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Chimplee, Siriphorn, Carl Smythe, Varomyalin Tipmanee, Suchada Sukrong та Kanyanatt Kanokwiroon. "Anticancer mechanism of 7-α-hydroxyfrullanolide on microtubules and computational prediction of its target binding in triple-negative breast cancer cells". PeerJ 10 (27 травня 2022): e13508. http://dx.doi.org/10.7717/peerj.13508.

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Background Triple-negative breast cancer (TNBC) responds poorly to the available drugs; thus, the mortality rate associated with TNBC remains high. 7-α-Hydroxyfrullanolide (7HF) possesses anticancer properties and arrests cells in the G2/M-phase via modulation of several proteins involved in the G2/M-phase transition, as well as the mitotic checkpoint in MDA-MB-468 (TNBC) cells. Microtubules (MTs) dynamically regulate cell division in the G2/M phase and are related to cancer cell stress response. However, antimitotic drug cytotoxicity to multiple cancer resistance developed in response to drugs are obstacles faced to date. Here, the activity and mechanism via which 7HF controls MTs dynamics was investigated in MDA-MB-468 cells. Methods 7HF uptake by MDA-MB-468 cells was assessed using spectrophotometry. The drug-like properties of 7HF were predicted using the Swiss-absorption, distribution, metabolism, and excretion (ADME) webtool. Then, the effect of 7HF treatment (6, 12, and 24 µM) on the dynamic arrangement of MTs was assessed for 1, 12, and 24 h using indirect immunofluorescence. Polymerization of α- and β-tubulin was assessed using different 7HF concentrations in a cell-free system for 1 h. Cell proliferation assay with bromodeoxyuridine plus propidium iodide staining and flow cytometry was performed at different 7HF concentrations and time points. The mechanism of action was assessed by detecting the expression of proteins, including Bub3, cyclin B1, p-Cdk1 (Tyr15), Rb, p-Rb (Ser780), Chk1, p-Chk1 (Ser345), Chk2, p-Chk2 (Ser516), and p-H2AX (Ser139), using western blotting. Molecular docking was used to predict the molecular interactions between 7HF and tubulins in MTs. Results We observed that 7HF was able to enter the MDA-MB-468 cells. The ADME webtool analysis predicted that it possesses the high passive permeation and gastrointestinal absorption properties of drugs. Various concentrations of 7HF disrupted the dynamic arrangement of spindle MTs by causing radial spindle array shrinkage and expansion of fibrous spindle density and radial array lengths in a time-dependent manner. 7HF reduced polymerization of α-, β-tubulin in dose-dependent manner. 7HF also triggered DNA damage response by inducing G2/M and G1 phase arrests in a concentration and time-dependent manner, which occurred due to the upregulation of Bub3, Chk1, p-Chk1 (Ser345), p-Cdk1 (Tyr15), and cyclin B1. According to molecular docking analysis, 7HF preferred to bind to β-tubulin over α-tubulin. The lactone, ketone, and hydroxyl groups of 7HF supported the 7HF-tubulin interactions. Hydrogen bonding with a hydrocarbon ring and salt bridge attractive forces were responsible for the binding versatility of 7HF. Conclusions This is the first study to investigate the molecular mechanism, MTs interacting sites, and the internalization and drug-like properties of 7HF in TNBC cells. The findings will be useful for developing 7HF-based treatment for patients with TNBC.
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Chimplee, Siriphorn, Sittiruk Roytrakul, Suchada Sukrong, Theera Srisawat, Potchanapond Graidist та Kanyanatt Kanokwiroon. "Anticancer Effects and Molecular Action of 7-α-Hydroxyfrullanolide in G2/M-Phase Arrest and Apoptosis in Triple Negative Breast Cancer Cells". Molecules 27, № 2 (9 січня 2022): 407. http://dx.doi.org/10.3390/molecules27020407.

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Triple negative breast cancer (TNBC) is a breast cancer subtype characterized by the absence of estrogen receptor, progesterone receptor and human epidermal growth factor receptor 2 expression. TNBC cells respond poorly to targeted chemotherapies currently in use and the mortality rate of TNBC remains high. Therefore, it is necessary to identify new chemotherapeutic agents for TNBC. In this study, the anti-cancer effects of 7-α-hydroxyfrullanolide (7HF), derived from Grangea maderaspatana, on MCF-7, MDA-MB-231 and MDA-MB-468 breast cancer cells were assessed using MTT assay. The mode of action of 7HF in TNBC cells treated with 6, 12 and 24 µM of 7HF was determined by flow cytometry and propidium iodide (PI) staining for cell cycle analysis and annexin V/fluorescein isothiocyanate + PI staining for detecting apoptosis. The molecular mechanism of action of 7HF in TNBC cells was investigated by evaluating protein expression using proteomic techniques and western blotting. Subsequently, 7HF exhibited the strongest anti-TNBC activity toward MDA-MB-468 cells and a concomitantly weak toxicity toward normal breast cells. The molecular mechanism of action of low-dose 7HF in TNBC cells primarily involved G2/M-phase arrest through upregulation of the expression of Bub3, cyclin B1, phosphorylated Cdk1 (Tyr 15) and p53-independent p21. Contrastingly, the upregulation of PP2A-A subunit expression may have modulated the suppression of various cell survival proteins such as p-Akt (Ser 473), FoxO3a and β-catenin. The concurrent apoptotic effect of 7HF on the treated cells was mediated via both intrinsic and extrinsic modes through the upregulation of Bax and active cleaved caspase-7–9 expression and downregulation of Bcl-2 and full-length caspase-7–9 expression. Notably, the proteomic approach revealed the upregulation of the expression of pivotal protein clusters associated with G1/S-phase arrest, G2/M-phase transition and apoptosis. Thus, 7HF exhibits promising anti-TNBC activity and at a low dose, it modulates signal transduction associated with G2/M-phase arrest and apoptosis.
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Mootz, Dietrich, and Klemens Bartmann. "Zur Kristallchemie von Supersäuren: Bildung und Struktur der tiefschmelzenden Addukte SbF5 · CF3SO3H und SbF5 · 7 HF [1] / Crystal Chemistry of Superacids: Formation and Structure of the Low-Melting Adducts SbF5 · CF3SO3H and SbF5 · 7 HF [1]." Zeitschrift für Naturforschung B 46, no. 12 (December 1, 1991): 1659–63. http://dx.doi.org/10.1515/znb-1991-1212.

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The melting diagram of the superacid system SbF5—CF3SO3H as well as of SbF5—HF has been studied by DTA and the crystal structure of one newly found low-melting adduct in each case determined. The adduct SbF5 · CF3SO3H (m.p. — 1°C, monoclinic, space group P21/c, Z = 4 formula units per unit cell) is composed of complex molecules F5SbOS(CF3)(O)OH which are linked by strong hydrogen bonds O-H ···F to infinite chains. The adduct SbF5 · 7HF (melting incongruently at —75°C, monoclinic, I 2/m, Z = 2) is a fluoronium salt, (H7F6)SbF6. The novel H7F6+ cation is formed as an unbranched chain by very strong hydrogen bonds between the respective F atoms, which become weaker from the center to the ends. A further hydrogen bond of this kind, between the cations and the octahedral SbF6- anions, again leads to a one-dimensional polymer.
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Larisang, Larisang, and Zeri Yusdinata. "Analisis Perbandingan Kondisi Existing dengan Metode Economic Order Quantity (EOQ) dalam menentukan Jumlah Persedian Bahan Baku WCP2 dan 7HF." JURNAL INDUSTRI KREATIF (JIK) 2, no. 2 (September 13, 2018): 19. http://dx.doi.org/10.36352/jik.v2i2.115.

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Jull, Laura G., Frank A. Blazich, and L. Eric Hinesley. "Seedling Growth of Atlantic White-Cedar as Influenced by Photoperiod and Day/Night Temperature." Journal of Environmental Horticulture 17, no. 3 (September 1, 1999): 107–13. http://dx.doi.org/10.24266/0738-2898-17.3.107.

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Abstract Seedlings of Atlantic white-cedar [Chamaecyparis thyoides (L.) B. S. P.] were grown in controlled-environment chambers for 12 weeks under short-or long-day conditions with 9-hr days at 18, 22, 26 or 30C (64, 72, 79 or 86F) in factorial combination with 15-hr nights at 14, 18, 22 or 26C (57, 64, 72 or 79F). Dry matter production was influenced by photoperiod and day/night temperature. For all day temperature × photoperiod interactions, except root:shoot ratio, growth was highest under long days. Day × night temperature interactions occurred for all growth measurements except root dry weight. Root dry weight was highest at 30/22C (86/72F); top (shoot) dry weight at 26/22C (79/72F). Nights of 14C (57F) resulted in the lowest top dry weight. Total plant dry weight was highest at nights of 22C (72F) for all day temperatures. At days of 30C (86F), total plant dry weight was highest with nights ≤ 22C (72F); however, data for 30/22C (86/72F) and 26/22C (79/72F) were similar. The highest root: shoot ratio occurred at nights of 14C (57F) with days ≤ 26C (79F). Mean relative growth rate was highest at nights of 22C (72F) with days of 26C (79F) or 30C (86F). Maximum stem caliper occurred at days of 22C (72F) with nights ≥ 18C (64F). Height and crown width were highest at 26/22C (79/72F). A day/night cycle of 30/22C (86/72F) with long days was optimal for seedling growth.
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Dalimunthe, Aminah, Poppy Anjelisa Zaitun Hasibuan, and Denny Satria. "The PI3KCA and AKT Inhibitory Activities of Litsea Cubeba Lour. Fruits and Heartwoods Towards Hela Cells." Open Access Macedonian Journal of Medical Sciences 7, no. 9 (May 14, 2019): 1422–24. http://dx.doi.org/10.3889/oamjms.2019.317.

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AIM: To investigated the activities of chloroform fractions at pH 7 of Litsea cubeba Lour. Fruits and heartwoods (CF-7F and CF-7H) in decrease expression of PI3KCA, Akt-1 and Akt-2 genes towards cervical cancer cell culture (HeLa) experiments in vitro. MATERIAL AND METHODS: CF-7F and CF-7H (12.5 and 25 µg/mL) were tested for its potential inhibition on gene expression of PI3KCA, Akt-1 and Akt-2 genes by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method. RESULT: CF-7F and CF-7H were showed the activity to reduce the expression of PI3KCA, Akt-1 and Akt-2 genes. CONCLUSION: Our results suggest that CF-7F and CF-7H significantly inhibit the expression of PI3KCA, Akt-1 and Akt-2 genes.
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Owen, James S., William A. Johnson, and Brian K. Maynard. "Effects of Auxin Concentration and Medium Temperature on Four Woody Plant Taxa Propagated by Stem Cuttings using Recirculating Subirrigation in a Growth Chamber." Journal of Environmental Horticulture 21, no. 3 (September 1, 2003): 126–30. http://dx.doi.org/10.24266/0738-2898-21.3.126.

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Abstract Four woody plant taxa (‘Sparkleberry’ holly, ‘Mariesii’ viburnum, ‘Shasta’ viburnum, and Red Sunset® maple) were propagated from softwood stem cuttings in a recirculating subirrigation propagation system to evaluate the effect on adventitious root formation of three auxin (Dip-n-Grow®) concentrations [0 (water), 20: 1, or 10: 1] and three medium temperatures [20C (68F), 23C (74F), or 26C (79F)]. All taxa showed a positive rooting response to auxin treatment. Compared to cuttings treated with water, root number of all taxa doubled when treated with the highest auxin concentration (10: 1 Dip-n-Grow® dilution). Length of the longest root of rooted cuttings increased with auxin treatment of two taxa. Medium temperature also affected rooting of all taxa. The intermediate temperature evaluated, 23C (74F), was optimal for all rooting parameters on cuttings of ‘Mariesii’ viburnum, and for root length and percentage rooting on cuttings of ‘Shasta’ viburnum and Red Sunset® maple. A propagation medium temperature of 26C (79F) resulted in the greatest root number on cuttings of ‘Shasta’ viburnum, while 20C (68F) was optimal for root number on cuttings of Red Sunset® maple. Root numbers on cuttings of ‘Mariesii’ viburnum were reduced at 20C (68F) and 26C (79F), which appeared to be sub-and supra-optimal temperatures, respectively, for root initiation. All rooting parameters on cuttings of ‘Sparkleberry’ holly increased with propagation medium temperature. Temperature-auxin interactions were apparent for root number and length on cuttings of ‘Mariesii’ and ‘Shasta’ viburnum. The recirculating subirrigation system was useful for evaluating the basal temperature and auxin dose interactions and was effective for rooting softwood stem cuttings without intermittent mist irrigation.
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Cseh, A., K. Kruppa, I. Molnár, M. Rakszegi, J. Doležel та M. Molnár-Láng. "Characterization of a new 4BS.7HL wheat–barley translocation line using GISH, FISH, and SSR markers and its effect on the β-glucan content of wheat". Genome 54, № 10 (жовтень 2011): 795–804. http://dx.doi.org/10.1139/g11-044.

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A spontaneous interspecific Robertsonian translocation was revealed by genomic in situ hybridization (GISH) in the progenies of a monosomic 7H addition line originating from a new wheat ‘Asakaze komugi’ × barley ‘Manas’ hybrid. Fluorescence in situ hybridization (FISH) with repetitive DNA sequences (Afa family, pSc119.2, and pTa71) allowed identification of all wheat chromosomes, including wheat chromosome arm 4BS involved in the translocation. FISH using barley telomere- and centromere-specific repetitive DNA probes (HvT01 and (AGGGAG)n) confirmed that one of the arms of barley chromosome 7H was involved in the translocation. Simple sequence repeat (SSR) markers specific to the long (L) and short (S) arms of barley chromosome 7H identified the translocated chromosome segment as 7HL. Further analysis of the translocation chromosome clarified the physical position of genetically mapped SSRs within 7H, with a special focus on its centromeric region. The presence of the HvCslF6 gene, responsible for (1,3;1,4)-β-d-glucan production, was revealed in the centromeric region of 7HL. An increased (1,3;1,4)-β-d-glucan level was also detected in the translocation line, demonstrating that the HvCslF6 gene is of potential relevance for the manipulation of wheat (1,3;1,4)-β-d-glucan levels.
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Glenn, Christopher T., Frank A. Blazich, and Stuart L. Warren. "Influence of Storage Temperatures on Long-term Seed Viability of Selected Native Ericaceous Species." Journal of Environmental Horticulture 16, no. 3 (September 1, 1998): 166–72. http://dx.doi.org/10.24266/0738-2898-16.3.166.

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Abstract Following harvest of capsules, drying, and seed extraction, seeds of Kalmia latifolia L. (mountain laurel), Leucothoe fontanesiana (Steud.) Sleum (drooping leucothoe), Rhododendron carolinianum Rehd. (Carolina rhododendron), Rhododendron catawbiense Michx. (Catawba rhododendron), and Rhododendron maximum L. (rosebay rhododendron) were stored for 0, 1, 2, 3, 4 or 5 years at −18,4 or 23C (0, 39 or 73F) and then germinated at 25C (77F) or an 8/16 hr thermoperiod of 25/15C (77/59F) with daily photoperiods of 0, 1 or 24 hr. Storage at −18 or 4C (0 or 39F) were most effective for maintaining seed viability of all species. After 5 years storage at −18 or 4C (0 or 39F), viability of L. fontanesiana, R. catawbiense, and R. maximum was relatively unchanged with total germination of 59%, 87%, and 88%, respectively. The same was noted for seeds of K. latifolia and R. carolinianum with total germination of 77% and 91%, respectively, after storage for 4 years at the same temperatures. Storage at 23C (73F) was the least effective for maintaining viability. After storage for 1 year at 23C (73F), germination decreased significantly for all species except R. carolinianum. By year 3, storage at 23C (73F) reduced seed viability of L. fontanesiana to essentially zero. The same occurred by year 4 for seeds of R. catawbiense and R. maximum stored at 23C (73F). Viability of K. latifolia also decreased under storage at 23C (73F) with germination of 14% noted by year 4. Viability of R. carolinianum did not decrease as rapidly as the other species when stored at 23C (73F) with total germination of 77% occurring by year 4. Regardless of storage duration, the photoperiod and temperature requirements for maximum germination of all species did not change.
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Lowder, Adam W., Helen T. Kraus, Frank A. Blazich, and Stuart L. Warren. "Day/Night Temperatures Influence Growth and Photosynthesis During Containerized Production of Selected Species of Helleborus (Hellebores)." Journal of Environmental Horticulture 28, no. 3 (September 1, 2010): 179–86. http://dx.doi.org/10.24266/0738-2898-28.3.179.

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Abstract Containerized seedlings of Helleborus foetidus L. (stinking hellebore), H. niger L. (Christmas rose), and H. ×hybridus L. (Lenten rose) were grown under long-day conditions in controlled-environment chambers for 95 days with 9-hr days of 14, 18, 22, 26, or 30C (57, 64, 72, 79, or 86F) in factorial combination with 15-hr nights of 10, 14, 18, 22, or 26C (50, 57, 64, 72, or 79F). Long-day conditions were provided by a 3-hr night interruption. Growth of each species responded differently to day and night temperatures. Calculated maximum root, top, and total dry weight, and leaf area of H. foetidus occurred with days/nights of 20/15, 18/13, 19/14, and 18/15C (68/59, 65/55, 66/57, and 65/59F), respectively. While night temperature (NT) had no effect on root:top ratio [RTR (root dry weight ÷ top dry weight)], RTR was greatest (0.65) with days of 22C (72F). Helleborus niger had calculated maximum root dry weight and total dry weight with days of 14C (57F) and nights of 16 and 13C (60 and 55F), respectively. Top growth of H. niger decreased linearly as NTs increased for days of 14 or 22C (57 or 72F). Day temperatures (DTs) had no effect on RTR, whereas RTR responded quadratically as NT increased with a calculated maximum RTR at nights of 19C (66F). Leaf area was maximized at days/nights of 14/10C (57/50F). At days of 22 or 26C (72 or 79F), top growth of H. ×hybridus responded quadratically as NT increased with maxima occurring at nights of 18 or 17C (64 or 63F). Root dry weight responded quadratically at days of 14, 22, or 26C (57, 72, or 79F) and calculated maxima occurred with nights of 18C (64F). At days of 22 or 26C (72 or 79F), there were quadratic responses in total dry weight with calculated maximum growth of H. ×hybridus at nights of 18 or 17C (64 or 63F), respectively. For days of 14, 22, or 30C (57, 72, or 86F), there were quadratic responses in RTR with greatest RTR calculated at nights of 15, 18, or 16C (59, 64, or 60F), respectively. There were quadratic responses at days of 22 or 26C (72 or 79F) for leaf area with calculated maxima at nights of 18 or 17C (64 or 63F), respectively. As DTs increased from 14 to 30C (57 to 86F) net CO2 assimilation (PN) of H. ×hybridus also increased linearly whereas increased NTs had no effect on PN. In contrast, stomatal conductance was not impacted by DT or NT.
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Дисертації з теми "7HF"

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Cromaz, Mario. "High-spin states in ¹§6§7Hf and ¹§7³§,¹§7§4W." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ27900.pdf.

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David, Karine. "Mise au point analytique de la mesure du rapport zr/hf dans les roches terrestres et extraterrestres par dilution isotopique en spectrometrie de masse a thermo-ionisation et a source plasma en multicollection. Utilisation des traceurs chimique zr/hf et isotopique #1#7#6hf/#1#7#7hf." Paris 7, 1997. http://www.theses.fr/1997PA077026.

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Анотація:
Apres avoir develloppe un protocole de purification du zirconium et de l'hafnium par chromatographie d'echange d'ions permettant de mesurer le rapport zr/hf par dilution isotopique (traceur double zirconium-hafnium) en spectrometrie de masse a thermoionisation (vg 354) et a source plasma avec un analyseur a double focalisation et une detection en multicollection (plasma 54), avec une precision a 2,5&, nous avons mesure les rapports chimique zr/hf et isotopique #1#7#6hf/#1#7#7hf dans des roches terrestres (basaltes oceaniques, roches et sediments continentaux) et extraterrestres (chondrites carbonees orgueil, allende et murchison). Les rapports zr/hf des roches terrestres sont significativement differents de la valeur chondritique. La gamme de variations des rapports zr/hf des basaltes de rides medio-oceaniques (morb) qui est similaire a celle du materiel continental montre que le rapport zr/hf a peu fractionne lors de l'extraction de la croute continentale. Afin d'identifier les differents processus (differenciation magmatique, fusion partielle et heterogeneites de sources) qui seraient succeptibles de fractionner le rapport zr/hf et d'expliquer les rapports plus eleves qui sont mesures dans les basaltes d'iles oceaniques, nous avons couple le rapport zr/hf avec differents traceurs geochimiques. L'etude effectuee sur les basaltes d'iles oceaniques differencies montre que le rapport zr/hf augmente condiserablement lors de la cristallisation de clinopyroxene. D'autre part, les rapports zr/hf plus eleves dans les oib que dans les morb suggerent egalement un fractionnement du rapport zr/hf lors de la fusion partielle, avec un coefficient de partage global solide/liquide du zirconium inferieur a celui de l'hafnium. A partir de l'etude couplee entre les rapports zr/hf et #1#7#6hf/#1#7#7hf effectuee sur l'ensemble des roches, nous suggerons que la presence de liquides carbonatitiques dans la source des oib produit un fractionnement important du rapport zr/hf lors de leur genese.
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Ambrosio, Simone Rodrigues. "Métodos bacteriológicos aplicados à tuberculose bovina: comparação de três métodos de descontaminação e de três protocolos para criopreservação de isolados." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-31102006-162337/.

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Анотація:
Dada a importância do Programa Nacional de Controle e Erradicação da Brucelose e Tuberculose (PNCEBT), a necessidade de uma eficiente caracterização bacteriológica dos focos como ponto fundamental do sistema de vigilância e as dificuldades encontradas pelos laboratórios quanto aos métodos de isolamento de Mycobacterium bovis fizeram crescer o interesse do meio científico por estudos, sobretudo moleculares, de isolados M. bovis. Para a realização dessas técnicas moleculares, é necessária abundância de massa bacilar, obtida através da manutenção dos isolados em laboratório e repiques em meios de cultura. Entretanto o crescimento fastidioso do M. bovis em meios de cultura traz grandes dificuldades para essas operações. Assim sendo, o presente estudo teve por objetivos: 1º) Comparar três métodos de descontaminação para homogeneizados de órgãos, etapa que precede a semeadura em meios de cultura, onde 60 amostras de tecidos com lesões granulomatosas, provenientes de abatedouros bovinos do Estado de São Paulo, foram colhidas e imersas em solução saturada de Borato de Sódio e transportadas para o Laboratório de Zoonoses Bacterianas do VPS-FMVZ-USP, onde foram processadas até 60 dias após a colheita. Essas amostras foram submetidas a três métodos de descontaminação: Básico (NaOH 4%), Ácido (H2SO4 12%) e 1- Hexadecylpyridinium chloride a 1,5% (HPC) e o quarto método foi representado pela simples diluição com solução salina (controle). Os resultados foram submetidos à comparação de proporções, pelo teste de χ², na qual verificou-se que o método HPC foi o que apresentou menor proporção de contaminação (3%) e maior proporção de sucesso para isolamento de BAAR (40%). 2º) Comparar três diferentes meios criopreservates para M. bovis, foram utilizados 16 isolados identificados pela técnica de spoligotyping. Cada um desses isolados foi solubilizado em três meios (solução salina, 7H9 original e 7H9 modificado), e armazenado em três diferentes temperaturas (-20ºC, -80ºC e -196ºC), sendo descongelado em três diferentes tempos (45, 90 e 120 dias de congelamento). Antes do congelamento e após o descongelamento foram feitos cultivos quantitativos em meios de Stonebrink Leslie. Os porcentuais de redução de Unidades Formadoras de Colônias (UFC) nas diferentes condições foram calculados e comparados entre si através de métodos paramétricos e não-paramétricos. Os resultados obtidos foram: na análise da variável tempo, em 90 dias de congelamento foi observada uma maior proporção de perda de M. bovis, quando comparado ao tempo 120 dias (p=0,0002); na análise da variável temperatura, foi observada uma diferença estatística significativa entre as proporções de perda média nas temperaturas de -20ºC e -80ºC (p<0,05); na análise da variável meio, foi observada uma diferença significativa (p=0,044) entre os meios A e C, para 45 dias de congelamento e -20ºC de temperatura de criopreservação. Embora as medianas dos porcentuais de perdas de UFC terem sido sempre inferiores a 4,2%, permitiram sugerir que o melhor protocolo de criopreservação de isolados de M. bovis é solubilizá-los em 7H9 modificado e mantê-los à temperatura de -20ºC
In the context of the National Program of Control and Eradication of Brucellosis and Tuberculosis (PNCEBT), the necessity of an efficient bacteriologic characterization of the infected herds as a cornerstone of the monitoring system and the difficulties faced by the laboratories regarding the methods for Mycobacterium bovis isolation led to a growing interest for scientific studies, especially molecular, of M. bovis isolates. To use these molecular techniques it is necessary to have an abundant bacillary mass, obtained through the maintenance of isolates in laboratory and replication in culture media. However the fastidious growth of M. bovis in culture media brings out great difficulties for these activities. Thus, the present study has the following objectives: First, to compare three decontamination methods for organ homogenates, phase that precedes the sowing in culture media, 60 samples of tissues with granulomatosis injuries, proceeding from bovine slaughterhouses in the State of São Paulo, were obtained, immersed in sodium borato saturated solution and transported to the Laboratório de Zoonoses Bacterianas of the VPS-FMVZ-USP, where they were processed up to 60 days after the sampling. These samples were submitted to three methods of decontamination: Basic NaOH 4%, Acid (H2SO4 12%) and 1- Hexadecylpyridinium chloride (HPC) 1.5% and a simple dilution with saline solution (control method). The results were analysed by means of the &chi test to compare proportions, and it was verified that HPC method presented the smallest proportion of contamination (3%) and the greatest proportion of success for M. bovis isolation (40%). Second, to compare three different cryopreservation media for M. bovis, 16 isolates identified by the technique of spoligotyping were used. Each one of these isolates was solubilized in three media (original saline solution, 7H9 and 7H9 modified), and stored in three different temperatures (-20ºC, -80ºC and -196ordm;C), and defrosted in three different time periods (45, 90 and 120 days of freezing). Before the freezing and after the unfreezing, quantitative cultivations in Stonebrink Leslie media were carried out. The proportions of Colony-Forming Units (CFU) loss in the different conditions were calculated and compared with one another through parametric and non-parametric methods. The results obtained were: in the analysis of the variable time, at 90 days of freezing a bigger proportion of CFU loss was observed when compared to 120 days (p=0,0002); in the analysis of the variable temperature, a statistically significant difference was observed between the average proportions of CFU loss for the temperatures of -20ºC and -80ºC (p<0,05); in the analysis of the variable media, a significant difference was observed (p=0,044) between the media A and C, for 45 days of freezing and -20ºC of cryopreservation temperature. Althougth the medium ones of the proportion of losses of CFU to always have been inferior 4,2%, had allowed to suggest that the best protocol for cryopreservation of M. bovis isolates is to solubilize them in 7H9 modified medium and to keep them at a temperature of -20ºC
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4

Low, David Wayne. "Application of transyt-7F on a downtown bus loop." Thesis, Georgia Institute of Technology, 1985. http://hdl.handle.net/1853/22343.

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5

Moreira, Catarina Alfaya. "DESENVOLVIMENTO DE PROTEÍNA SINTÉTICA QUIMÉRICA BASEADA EM TALE (TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR) PARA APLICAÇÃO EM SISTEMA BIOSSENSOR DE DNA. 72f 2017." Instituto de Ciências da Saúde, 2017. http://repositorio.ufba.br/ri/handle/ri/25293.

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As abordagens de Biologia Sintética possuem potencial de aumentar a produção de ferramentas diagnósticas adequadas para utilização na prática, como os biossensores de DNA. O controle dos sistemas biológicos permite o aprimoramento de tecnologias em uso e surgimento de novas tecnologias, como o diagnóstico molecular através da identificação de sequências específicas de DNA. A maioria dos métodos moleculares de diagnóstico de DNA se baseiam em hibridização. Contudo, atualmente, as proteínas ligadoras de DNA tem tido destaque, devido às suas numerosas vantagens. As principais vantagens são a identificação de sequências em DNA dupla-fita, eliminando o passo de desnaturação, e a alta especificidade pelo alvo. Neste contexto, o objetivo deste trabalho foi desenvolver uma proteína sintética quimérica para aplicação em sistema biossensor de DNA que permitirá rápida identificação de uma sequência específica de DNA de Corynebacterium striatum, um importante patógeno emergente. Foi construída, neste estudo, uma proteína quimérica biossensora composta por: (I) um domínio de ligação ao DNA derivado de proteínas TALEs (proteínas efetoras do tipo ativadores transcricionais) o qual reconhece especificamente uma sequência de 18 pb de um gene único de C.striatum codificador de uma permease para transporte de ferro; combinado a (II) uma cromoproteína tsPurple (Tinsel Purple) para permitir detecção visual da sequência alvo. Para construção do gene codificador da proteína TALE específica, o gene específico de C. striatum foi analisado com a ferramenta Tal Effector Nucleotide Targeter (Cornell University) e uma sequência-alvo de 18pb foi definida. A construção de domínios TAL foi realizada utilizando a ferramenta GeneArtTM Perfect Match TAL (Thermo Scientific); o gene sintético encontra-se clonado no plasmídeo pTALE. Para construir as sequências gênicas quiméricas, foram desenhados primers de PCR para isolamento da sequência da tsPurple com a adição de sítios artificiais de restrição para EcoRI e BamHI; os produtos de PCR digeridos foram clonados no plasmídeo pTALE, downstream à sequência da TALE específica. Essas sequências fusionadas foram então sub-clonadas em um vetor de expressão pDESTTM17, através de recombinação utilizando tecnologia Gateway para expressão das proteínas quiméricas. A expressão da proteína quimérica foi induzida pela adição de 1mM de IPTG. A expressão da proteína quimérica foi confirmada através de análises de SDS PAGE 10% , que demonstraram a superexpressão da proteína esperada de 151kDa; além disso, foi observada a forte coloração roxa no precipitado bacteriano, em razão da funcionalidade da cromoproteína TsPurple.
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6

Moskaluk, John. "Arterial priority option for the TRANSYT-7F traffic-signal-timing program." Diss., Georgia Institute of Technology, 1987. http://hdl.handle.net/1853/19428.

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7

Touati, Fayçal. "Intégration d'antennes sur silicium en technologie BiCMOS pour des objets communicants de faible puissance au delà de 10 GHz." Grenoble INPG, 2006. http://www.theses.fr/2006INPG0032.

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Les Objets Communicants sont des dispositifs dotés de capacités de communication et de traitement de l'information. La transmission RF des données se fait souvent dans des milieux confinés (propagation indoor) et dans des bandes de fréquences au-delà des standards de la téléphonie mobile. De nos jours, les puces à front-end radio assurant la communication comprennent des éléments passifs tels que les inductances et les condensateurs, l'idée d'intégrer (intégration au sens microélectronique) la partie antenne et sa logique de commutation semble être une piste intéressante pour des applications à des fréquences élevées, dans le but de proposer des dispositifs RF destinés aux Objets Communicants. Aussi, cette intégration permettrait de disposer d'un seul processus de fabrication, limitant de ce fait l'adjonction d'éléments externes réduisant d'autant les coûts de fabrication du fait que la partie radio serait réalisée d'un seul bloc. Le but de la thèse est de simuler, concevoir, et caractériser expérimentalement des antennes dipôles intégrées et couplées à des VCO sur des puces en silicium en utilisant des technologies « standards» du type BiCMOS. La gamme de fréquences envisagées va de 10 GHz à 20 GHz. Il s'agit au terme de cette étude, d'être en mesure de déterminer les effets des paramètres technologiques du substrat silicium sur les performances de rayonnement de l'antenne intégrée, et de proposer des solutions d'intégration qui optimisent les performances de cet élément
The RF transmission of the data are subject to indoor propagation at high frequency bands, beyond mobile telephony spectrum standards. Nowaday, the onchip radio front-end contains passive components such like, spiral inductors and capacitors. Thinking about integrating (in terms of microelectronic integration) the antenna including its switching capabilities, seems to be interesting in the field of Smart Deviees operating at high frequency ranges. The aim of this study, is to simulate, design and measure dipole antennas directly coupled to VCO's, using standard (industrial type) silicon BiCMOS technology. The considered operating frequencies are in the range of 10 to 20 GHz. By achieving this study, the technological issues for antenna integration will be highlighted and the capabilities of the considered standard BiCMOS microelectronic technologies will be investigated to allow on-chip antenna implementation with acceptable performances
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Skaza, Fore. "Structure du noyau exotique 8HE par les réactions directes 8HE(P,P')8HE, 8HE(P,D)7HE et 8HE(P,T)6HE." Paris 11, 2004. http://www.theses.fr/2004PA112173.

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Les réactions directes de diffusions élastique et inélastique d'un faisceau d'8He sur une cible de protons ont été mesurées au GANIL. Le faisceau d'8He produit par le dispositif SPIRAL à une énergie de 15. 6 A. MeV était envoyé sur une cible de protons. Le dispositif expérimental était composé de l'ensemble des huit télescopes MUST pour la détection des particules légères de recul, d'un ensemble de six lattes de scintillateurs plastiques pour la détection des éjectiles lourds et de deux détecteurs de faisceaux CATS pour la reconstruction événement par événement de la position et de l'angle d'incidence du faisceau sur la cible. Ce dispositif a aussi permis de mesurer les réactions de transfert d'un neutron vers l'7He et de deux neutrons vers l'6He. Les spectres en énergie d'excitation des 6,7,8He ont été mesurés ainsi que les distributions angulaires associées à chaque voie de réaction. Les états observés pour les trois noyaux sont en accord avec les données de la littérature. Nous indiquons la présence d'un premier état excité dans l'7He à 0. 9 MeV et nous donnons aussi pour la première fois la position d'un second état excité de l'8He à 5. 4 MeV, état dont la présence était seulement suggérée par une expérience précédente. Des calculs CCBA ont permis d'extraire un facteur spectroscopique de 4. 4±1. 3 pour le transfert d'un neutron de l'8He vers l'7He, valeur en accord avec une sous-couche p3/2 fermée pour l'8He. Pour l'analyse des distributions angulaires de diffusion élastique et inélastique, il s'est avéré nécessaire de prendre en compte explicitement dans le formalisme, via des calculs en voies couplées, les couplages à la réaction de transfert d'un neutron
The elastic and inelastic scattering reactions of an 8He beam on a proton target were measured at GANIL. The 8He beam produced by the SPIRAL facility at an energy of 15. 6 A. MeV impinged on a proton target and the recoil proton was detected in an array of eight telescopes, the wall of strip-detectors, MUST, devoted to the detection of light charged. The experimental setup was also by a plastic scintillator wall for the detection of heavy projectile, and by two beam tracking detectors CATS for the measure event by event of the incident position and angle of the beam on the target. This setup allowed also to measure the one and two neutron transfer reactions 8He(p,d)7He and 8He(p,t)6He. The excitation energy spectrum for 6,7,8He and the angular distributions associated to each reaction were measured. The observed excited states are in agreement with the data of the literature. We indicate the presence of a first excited state for 7He at 0. 9 MeV and we give for the first time the position of a second excited state in 8He; the presence of such state was just suggested in a previous experiment. From CCBA calculations a spectroscopic factor of 4. 4±1. 4 for the pick-up of one neutron from 8He to 7He was extracted from the data. This value is in agreement with a closed p3/2 sub-shell for 8He. To analyse successfully the angular distributions for elastic and inelastic scattering, it was necessary to take into account in the formalism, used to describe the reactions, the couplings to the one neutron transfer reaction. This was done via coupled channels reactions calculations
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Poblete, González Cristián Ignacio. "Estudio de las especies transientes generadas durante la fotorreducción por aminas de oxoisoaporfinas : 5-metoxi-7H-dibenzo[de,h]quinolin-7-ona y 7H-dibenzo[de,h]quinolin-7-ona." Tesis, Universidad de Chile, 2007. http://repositorio.uchile.cl/handle/2250/105633.

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Memoria para optar al título de Químico Farmacéutico
Los sistemas fotosintéticos naturales y artificiales en los cuales la energía de la luz es colectada y almacenada como energía química, es un proceso de conversión que es iniciado con la transferencia fotoinducida de un electrón, que es seguida por otras etapas químicas que permiten la separación y estabilización cinética de los llamados “sistemas de carga separada”. Este es el caso de las 7H-dibenzo[de,h]quinolin-7-onas, conocidas como oxoisoaporfinas, que al ser fotorreducidas en presencia de aminas generan fotoproductos iónicos metaestables, capaces de almacenar una fracción apreciable de la energía de fotoexcitación. Los fotoproductos que se forman vía una transferencia secuencial de electrón-protón-electrón, revierten térmicamente a las oxoisoaporfinas originales, generando productos de oxidación de la amina. La reconversión del fotoproducto metaestable permite la reutilización del sistema en tanto exista amina remanente que reaccione con la oxoisoaporfina regenerada. Se estudiaron los transientes de la fotorreducción por aminas de la oxoisoaporfina y 5-metoxi-oxoisoaporfina, el triplete excitado, el anión radical y el radical neutro. Los intermediarios transientes fueron generados por láser flash-fotólisis, en presencia y ausencia de aminas terciarias, con y sin hidrógenos alfa. Si la amina no es capaz de donar protones, la reacción procederá sólo hasta la formación de A-·. Se identificaron y se caracterizaron los tripletes y radicales de las dos oxoisoaporfinas en estudio y se determinaron los parámetros cinéticos
Natural and artificial photosynthetic systems in which the energy of the light is collected and stored as chemical energy, is a process beggining with a photoinduced single electron transfer, PET, followed by other stages wich allow the separation and kinetic stabilization of “separate-charge systems”. This is the case of 7H-dibenzo[de,h]quinolin-7-onas, well-known as oxoisoaporphines, wich are photoreduced by amines, generating ionic metastable photoproducts capable to store an appreciable fraction of the photoexcitation energy. The photoproducts, formed via a sequential transfer of electron-proton-electron, revert thermally to the original oxoisoaporphines generating amine photooxidation products. The back reaction of the metastable photoproducts allows the reuse of the system as long as amine remains in the system, to react with the regenerated oxoisoaporphine. In this work we studied the transient species involved in the photoreduction by amines of oxoisoaporphine and 5-methoxy-oxoisoaporphine; the excited triplet state of oxoisoaporphines, the radical anion formed after electron transfer and the neutral hydrogen radical, generated by proton transfer. Photoreduction intermediary transients were generated by laser flash-photolysis, in the presence and absence of tertiary amines, with and without transferable α- hydrogen. If the amine is not able to donate protons, the reaction will only proceed until the formation of A-·, allowing its characterization. With amines capables to donate an α- H, photoreduction continues to the formation of neutral hydrogen radical AH·, and eventually to metastable photoproducts. In this work triplet excited states were characterized, radical anions and neutral hydrogen radicals for both oxoisoaporphines and the kinetic parameters through evolve were also determined
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SINER, ANGELA. "In vivo metabolism of 7H-dibenzo[c.g.] carbazole (DBC) and benzo[a]pyrene (BaP)." University of Cincinnati / OhioLINK, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1014752553.

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Книги з теми "7HF"

1

Instruments, Texas. SN54/74F logic data book. 2nd ed. [Freising]: [Texas Instruments Deutschland], 1987.

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2

Instruments, Texas. F logic data book (SN54/74F). Dallas: Texas Instruments, 1994.

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3

Instruments, Texas. 74F-logic data book: SN54/SN74F. (s.l.): Texas Instruments, 1987.

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4

Archives départementales de la Savoie. Henry Bordeaux: Inventaire-index de la correspondance reçue, 1891-1973 (73F). [Chambéry?]: Conseil général de la Savoie, 1993.

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5

National Defense University. Center for Technology and National Security Policy, ed. The 71F advantage: Applying Army research psychology for health and performance gains. Washington, D.C: Published for the Center for Technology and National Security Policy by National Defense University Press, 2010.

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6

Aṭibbāʼ Baghdādīyūn mutamayyizūn fī al-qarnayn 6 wa 7H/12 wa 13M. Baghdād: Dār al-Shuʼūn al-Thaqāfīyah al-ʻĀmmah, 2016.

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7

Benjamin, Walter. Trois pièces radiophoniques. [Paris]: C.Bourgois, 1987.

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8

Kūrdistān: Dirāsah fī al-nuẓum al-tijārīyah wa-al-mālīyah (min al-qarn 4-7H/10-13M). Kūyah, [Irbīl]: Markaz al-Abḥāth wa-al-Nashr, 2011.

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9

Parker, Lloyd E. G. Preliminary testing and evaluation of new computer programs for traffic analysis: Task, evaluation of supercomputer potential. Downsview, Ont: Research and Development Branch, Ontario Ministry of Transportation, 1990.

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10

Ben, Jonson. La foire de la Saint-Barthélemy. Montréal: Leméac, 1994.

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Частини книг з теми "7HF"

1

Dorgelo, Marlou. "7f Mondhygiëne." In Klinisch redeneren bij ouderen, 224–37. Houten: Bohn Stafleu van Loghum, 2017. http://dx.doi.org/10.1007/978-90-368-1487-4_12.

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2

Meister, M., D. Aleksandrov, T. Aumann, L. Axelsson, T. Baumann, M. J. G. Borge, L. V. Chulkov, et al. "Low-lying resonance states in 7He." In Exotic Nuclei and Atomic Masses, 227–28. Berlin, Heidelberg: Springer Berlin Heidelberg, 2003. http://dx.doi.org/10.1007/978-3-642-55560-2_81.

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3

Dorgelo, Marlou. "7h Decubitus en huidletsel." In Klinisch redeneren bij ouderen, 255–79. Houten: Bohn Stafleu van Loghum, 2017. http://dx.doi.org/10.1007/978-90-368-1487-4_14.

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4

Winkelmann, Jochen. "Diffusion coefficient of 7H-benzanthracen-7-one into hexane and acetic acid ethyl ester solution." In Diffusion in Gases, Liquids and Electrolytes, 1593. Berlin, Heidelberg: Springer Berlin Heidelberg, 2017. http://dx.doi.org/10.1007/978-3-540-73735-3_1358.

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5

Teranishi, Katsunori, Saori Tanabe, Atsuko Komoda, Makoto Hisamatsu, and Tetsuya Yamada. "Synthesis and Enhanced Chemiluminescence of New Mono-Cyclomaltooligosaccharide-Bound 6-Phenylimidazo[1,2-a]Pyrazin-3(7H)-Ones." In Proceedings of the Ninth International Symposium on Cyclodextrins, 153–56. Dordrecht: Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-011-4681-4_36.

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6

Roesky, H. W., J. Lucas, and Arland D. Norman. "1,3,5,7-Tetramethyl-1H ,5H -[1,4,2,3]Diazadiphospholo-[2,3-B ][1,4,2,3]Diazadiphosphole-2,6(3H ,7H )-Dione and a Molybdenum Complex." In Inorganic Syntheses, 122–25. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2007. http://dx.doi.org/10.1002/9780470132555.ch39.

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7

Gangjee, A., Y. Zeng, J. J. McGuire, R. L. Kisliuk, and E. Chu. "Synthesis of N-[4-[(2-Amino-6-Methyl-3,4-Dihydro-4-Oxo-7H-Pyrrolo[2,3-d]-Pyrimidin-5-Yl)Methyl]Benzoyl]-L-Glutamic Acid as an Antifolate." In Chemistry and Biology of Pteridines and Folates, 439–43. Boston, MA: Springer US, 2002. http://dx.doi.org/10.1007/978-1-4615-0945-5_74.

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8

Hirota, E., K. Kuchitsu, T. Steimle, J. Vogt, and N. Vogt. "222 C14H8O6 8b,8c-Dihydro-8b,8c-dimethyl-1H,3H,5H,7H-furo[3'',4'':1',3']cyclopropa[1',2',3':3,4]pentaleno[1,6-cd]pyran-1,3,5,7-tetrone." In Molecules Containing Three or Four Carbon Atoms and Molecules Containing Five or More Carbon Atoms, 409–10. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-41504-3_353.

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9

"7he." In 'Eliza', 92–109. Routledge, 2017. http://dx.doi.org/10.4324/9781315256818-10.

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"§ 75f [Sperrabrede]." In §§ 17-83, 970–78. De Gruyter, 2022. http://dx.doi.org/10.1515/9783111097510-063.

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Тези доповідей конференцій з теми "7HF"

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Wuosmaa, A. H. "Excited States In 7He From d(6He,p)7He." In NUCLEI AT THE LIMITS. AIP, 2005. http://dx.doi.org/10.1063/1.1905341.

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Goldmeer, Jeffrey, William York, and Paul Glaser. "Fuel and Combustion System Capabilities of GE’s F and HA Class Gas Turbines." In ASME Turbo Expo 2017: Turbomachinery Technical Conference and Exposition. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/gt2017-64588.

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Анотація:
The world is currently undergoing a shift in the power industry with an increase in the amount of electrical power being generated from natural gas. This is being driven, in part, by the increase in availability of natural gas derived from shale gas and the reduction in global natural gas prices. In parallel, there is a second trend; a shift of new gas turbines installations to higher efficiency F and HA-class gas turbine combined cycle platforms. Supporting these industry trends, GE’s DLN 2.6+ combustion system, which is available for the 7F, 9F, 7HA, and 9HA gas turbines offers a high degree of operational and fuel flexibility. The latest evolution of this combustion technology for GE’s 7F gas turbines has been commissioned on 25 units, including new units and upgrades to existing turbines. The increased fuel and operational flexibility of this system is aided by GE’s advanced control systems that includes a high fidelity digital twin. This paper details recent developments of the DLN 2.6+ combustion system as well as validation examples for both the DLN 2.6+ and the digital twin controls system.
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WOLSKI, R., Yu M. TCHUVIL'SKY, D. D. BOGDANOV, M. S. GOLOVKOV, A. S. FOMICHEV, A. M. RODIN, S. V. STEPANTSOV, S. I. SIDARCHUK, G. M. TER-AKOPIAN, and Yu Ts OGANESSIAN. "CLUSTERING OF 8He AND 7He TESTED BY 8He + p TRANSFER REACTIONS." In Proceedings of the International Symposium on Post-Symposium of YKIS01. WORLD SCIENTIFIC, 2002. http://dx.doi.org/10.1142/9789812777577_0003.

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Demyanova, Alla S., Boris A. Chernyshev, Sergey A. Gohcharov, Yury B. Gurov, Sergey V. Lapushkin, Alexey A. Ogloblin, and Vyacheslav G. Sandukovsky. "Halo-like Structure of 7He and Transition from Neutron Halo to Neutron Skin." In Proceedings of 13th International Conference on Nucleus-Nucleus Collisions. Journal of the Physical Society of Japan, 2020. http://dx.doi.org/10.7566/jpscp.32.010026.

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Karim, Hasan, Jayaprakash Natarajan, Venkat Narra, Jun Cai, Shreekrishna Rao, Jonathan Kegley, and Joseph Citeno. "Staged Combustion System for Improved Emissions Operability and Flexibility for 7HA Class Heavy Duty Gas Turbine Engine." In ASME Turbo Expo 2017: Turbomachinery Technical Conference and Exposition. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/gt2017-63998.

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Driven by global warming, a relentless march towards increased fuel efficiency has resulted in increased firing temperature for HA-class engines without an increase in baseload emissions. Moreover, emissions compliance for CO, NOx, and unburned hydrocarbons are desired over increased range in gas turbine load. In addition, exceptional gas turbine operational flexibility is desired to address potential intermittency due to the penetration of renewables in the electrical grid. Staged/sequential combustion is a state of the technology to provide operational flexibility and reduced emissions in power generation gas turbines. GE Power’s 7HA-class gas turbine combustion system combines GE’s proven DLN-2.6+ combustion technology, that has run reliably for over 1.3 million fired hours across more than eighty 9FA.03, 9F.05 & 7FA gas turbine engines, with an axially fuel staged system (AFS). Axially staging combustion to two zones allows for increased firing temperature at baseload (while maintaining the same NOx level) by operating the later/second stage hotter than the first/primary stage. During low load operation as the gas turbine firing temperature is reduced, percentage fuel split in the staged fuel system can either be reduced significantly or turned off and thereby keeping the overall combustion system into emissions compliance over a wider range of firing temperatures. This paper presents both the development testing of the staged combustion in the FA and HA class gas turbine combustion system rigs at GE Power’s Gas Turbine Technology Laboratory and the validation testing of staged combustion system for the 7HA.01 engine completed during Spring 2016 at GE Power’s engine test facility in Greenville, SC. The paper also discusses the significant simplification of operational principle and flexibility of startup, loading and baseload operation of the 7HA combustion system. Discussion of engine test results will show how axial fuel staging was utilized to demonstrate emissions compliance ( NOx (15% O2) < 25 ppm; CO < 9 ppm), operation from 14% load to 100% load with low combustion dynamics and also to enable wide wobbe capability, which is a normalized measure of fuel flexibility.
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Hansen, D. L., S. Pong, P. Rosenthal, and J. Gorelick. "Total Ionizing Dose Testing of SiGe 7HP discrete Heterojunction Bipolar Transistors for ELDRS Effects." In 2007 IEEE Radiation Effects Data Workshop. IEEE, 2007. http://dx.doi.org/10.1109/redw.2007.4342569.

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Caamaño, M., D. Cortina, C. E. Demonchy, B. Jurado, W. Mittig, F. Rejmund, M. Rejmund, et al. "Production and Characterization of the 7H Resonance." In INTERNATIONAL SYMPOSIUM ON EXOTIC NUCLEI. AIP, 2007. http://dx.doi.org/10.1063/1.2746577.

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Feigl, Markus, Geoff Myers, Stephen R. Thomas, and Raub Smith. "7H™ Combustion System Performance With Fuel Moisturization." In ASME Turbo Expo 2006: Power for Land, Sea, and Air. ASMEDC, 2006. http://dx.doi.org/10.1115/gt2006-90281.

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This paper describes the concept and benefits of the fuel moisturization system for the GE H System™ steam-cooled industrial gas turbine. The DLN2.5H combustion system and fuel moisturization system are both described, along with the influence of fuel moisture on combustor performance as measured during full-scale, full-pressure rig testing of the DLN2.5H combustion system. The lean, premixed DLN2.5H combustion system was targeted to deliver single-digit NOx and CO emissions from 40% to 100% combined cycle load in both the Frame 7H (60 Hz) and Frame 9H (50 Hz) heavy-duty industrial gas turbines. These machines are also designed to yield a potential combined-cycle efficiency of 60 percent or higher. Fuel moisturization contributes to the attainment of both the NOx and the combined-cycle efficiency performance goals, as discussed in this paper.
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Freitag, I., S. Knoke, A. Tünnermann, and H. Welling. "Injection-Locked Nd:YAG High Power Lasers Pumped by Fiber-Coupled Diode Lasers." In The European Conference on Lasers and Electro-Optics. Washington, D.C.: Optica Publishing Group, 1996. http://dx.doi.org/10.1364/cleo_europe.1996.cma3.

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For Michelson-type gravitational wave detectors a strain sensitivity of at least 10-21 is desired. This ultra-high sensitivity places challengingly high performance requirements on the laser light source. The laser has to work at high power levels in single-frequency operation (tens of Watts) with excellent frequency stability (FM-noise < 3·10-7Hz/√Hz ) , and intensity noise as close as possible to the quantum noise limit. Beyond that, the lifetime of this system must be in the range of several 10.000h at low operational costs. We report on the development towards a diode-pumped Nd:YAG laser system, based on the injection locking technique, that fulfills these requirements.
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Joshi, Siddharth, Chul Kim та Gert Cauwenberghs. "A 6μW/MHz charge buffer with 7fF input capacitance in 65nm CMOS for non-contact electropotential sensing". У 2016 IEEE International Symposium on Circuits and Systems (ISCAS). IEEE, 2016. http://dx.doi.org/10.1109/iscas.2016.7539210.

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Звіти організацій з теми "7HF"

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Matsumura, Akihiko. Spectroscopy of 28Al(Lambda), 12B(Lambda), 7He(Lambda) by the (e,e'K+) Reaction. Office of Scientific and Technical Information (OSTI), January 2009. http://dx.doi.org/10.2172/992499.

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Hodgson, D. A., and J. Bednarski. Preliminary surficial materials of Kagloryuak River (77F) and Burns Lake (77G), Victoria Island, Northwest Territories. Natural Resources Canada/ESS/Scientific and Technical Publishing Services, 1994. http://dx.doi.org/10.4095/194078.

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McCurdy, M. W., R. J. McNeil, Y. E. Abramovitch, and S. J. A. Day. Regional lake sediment geochemical data, Nonacho Basin - east arm of Great Slave Lake region, Northwest Territories (NTS 75C and 75F). Natural Resources Canada/ESS/Scientific and Technical Publishing Services, 2012. http://dx.doi.org/10.4095/292064.

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ARMY ENGINEER DISTRICT ROCK ISLAND IL. Upper Mississippi River System Environmental Management Program Definite Project Report (R-7F) with Integrated Environmental Assessment, Lake Chautauqua. Fort Belvoir, VA: Defense Technical Information Center, June 1991. http://dx.doi.org/10.21236/ada251891.

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Kanasewich, E. R., and Z. Berkes. Reprocessed and Interpreted Seismic Reflection Data From the Arctic Platform, Parry Islands Fold Belt, and the Sverdrup Basin of eastern Melville Island, Canadian Arctic Islands: a Study of Geophysical Data Acquired By Industry Within Portions of NTS 78F, 78G, 79B and 88E. Natural Resources Canada/ESS/Scientific and Technical Publishing Services, 1988. http://dx.doi.org/10.4095/130495.

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Aeromagnetic total field map, 77F/SW, Victoria Island, NWT. Natural Resources Canada/ESS/Scientific and Technical Publishing Services, 1998. http://dx.doi.org/10.4095/215851.

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Aeromagnetic total field map, 77F/NW, Victoria Island, NWT. Natural Resources Canada/ESS/Scientific and Technical Publishing Services, 1998. http://dx.doi.org/10.4095/215852.

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Uranium map (eU), map 96-7F, Nepisiguit Lakes, New Brunswick. Natural Resources Canada/ESS/Scientific and Technical Publishing Services, 1996. http://dx.doi.org/10.4095/216590.

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Uranium / Thorium map, map 96-7H, Nepisiguit Lakes, New Brunswick. Natural Resources Canada/ESS/Scientific and Technical Publishing Services, 1996. http://dx.doi.org/10.4095/216608.

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Environmental, Health and Safety Assessment: ATS 7H Program (Phase 3R) Test Activities at the GE Power Systems Gas Turbine Manufacturing Facility, Greenville, SC. Office of Scientific and Technical Information (OSTI), November 1998. http://dx.doi.org/10.2172/1753.

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