Дисертації з теми "Zooxanthelles"
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Denis, Vianney. "Capacités et modalités d’adaptation de deux espèces de coraux zooxanthellés aux perturbations climatiques et anthropiques (île de la Réunion, Sud-Ouest de l’océan Indien)." Electronic Thesis or Diss., La Réunion, 2010. https://tel.archives-ouvertes.fr/tel-04058955.
Повний текст джерелаReef coral communities will undergo major changes in the next decades. The potentials of acclimatization and adaptation to environmental changes are compared between two zooxanthellate scleractinian corals dominant on Reunion coral reefs: the K-strategist Porites lutea and the r-strategist Acropora muricata. Different traits of the holobionts (survival, growth, regeneration, tissue biomass, protein content, lipid composition) and their zooxanthellae (genetic identity, photosynthetic parameters) are characterized in situ in two to four shallow reef flat sites, less than 11 km apart. Their environmental conditions offer a wide range of temperature, light, hydrodynamism and nutrient levels. P. lutea which is associated to the thermotolerant zooxanthellae Symbiodinium C15 has a high potential for acclimatization. After transplantation to a new environment, P. lutea quickly adjusts its growth and protein content, without suffering any mortality. In contrast, A. muricata, which is combined with the thermosensitive zooxanthellae C2/C3, does not display such a capacity for acclimatization and showed a high mortality. All the characteristics (except tissue biomass) of A. muricata and photosynthetic parameters, as well as tissue biomass of P. lutea, are marked by an "imprint" of the original site. This limited phenotypic plasticity suggests a genetic differentiation at small-scale. In A. muricata, it results in an increased tolerance to high temperatures in the most fluctuating environment. A. muricata also shows greater regenerative capacities than P. lutea. In the latter species, regeneration is correlated to solar radiation and temperature, through their control of the photosynthetic performance of symbiotic zooxanthellae. A seasonal change in autotrophy vs heterotrophy is detected in A. muricata at the site where exposition to oceanic environment is the highest. The phenotypic plasticity of P. lutea, a long-lived species, allows it to acclimatize to changing environmental conditions. Recovery capacities of A. muricata, in relation to its adaptive capacity to local conditions, would also allow this opportunistic species to live through the environmental changes that are expected in the context of global change, but within limits yet to be defined for these two scleractinian species
REYNAUD, VAGANAY STEPHANIE. "Controle environnemental de la physiologie et de la composition isotopique du squelette des scleractiniaires a zooxanthelles : approche experimentale." Nice, 2000. http://www.theses.fr/2000NICE5406.
Повний текст джерелаGattuso, Jean-Pierre. "Ecomorphologie, métabolisme, croissance et calcification du scléractiniaire à zooxanthelles Stylophora pistillata (Golfe d'Agaba, Mer Rouge) influence de l'éclairement /." Grenoble 2 : ANRT, 1987. http://catalogue.bnf.fr/ark:/12148/cb37605301r.
Повний текст джерелаAL-MOGHRABI, SALIM. "Metabolisme et transport des nutriments dans un modele d'association symbiotique animal-vegetal : les microcolonies d'un scleractiniaire a zooxanthelles galaxea fascicularis." Nice, 1992. http://www.theses.fr/1992NICE4588.
Повний текст джерелаMarchioretti, Manuel. "Nouvelles données écophysiologiques chez les scléractiniaires à zooxanthelles du genre stylophora(Schweigger,1819) : perspectives d'applications à la restauration des récifs coralliens." Nice, 1999. http://www.theses.fr/1999NICE5271.
Повний текст джерелаGOIRAN, CLAIRE. "La symbiose entre les scleractiniaires et les dinoflagelles : physiologie des zooxanthelles symbiodinium sp. du corail galaxea fascicularis, hors de l'association symbiotique." Nice, 1994. http://www.theses.fr/1994NICE4800.
Повний текст джерелаHill, Ross. "Coral bleaching : photosynthetic impacts on symbiotic dinoflagellates /." Electronic version, 2008. http://hdl.handle.net/2100/526.
Повний текст джерелаGlobal climate change is leading to the rise of ocean temperatures and is triggering mass coral bleaching events on reefs around the world. This involves the expulsion of the symbiotic dinoflagellate algae, known as zooxanthellae, from the coral host. Coral bleaching is believed to occur as a result of damage to the photosynthetic apparatus of these symbionts, although the specific site of initial impact is yet to be conclusively resolved. This thesis examined a number of sites within the light reactions of photosynthesis and evaluated the efficiency of photoprotective heat dissipating pathways. Upon expulsion, the capacity for long-term survivorship of expelled zooxanthellae in the water column was also assessed. A reduction in photosystem II (PSII) photochemical efficiency during exposure to elevated temperature and high light (bleaching conditions) was found to be highly dependent upon the increase in abundance of QB non-reducing PSII centres (inactive PSII centres), indicating damage to the site of the secondary electron acceptor, QB, resulting in a limited capacity for its reduction. Therefore, this reduced the rate of the reoxidation of the primary electron acceptor, QA-. Fast induction curve (FIC) analysis of the rise from minimum fluorescence to maximum fluorescence revealed a lower amplitude in the J step along this curve, which was consistent with a reduction in the rate of QA reoxidation. This photoinhibition of PSII was found to occur once the effectiveness of excess energy dissipation through energy-dependent quenching and state-transition quenching was exceeded, suggesting that these mechanisms were incapable of preventing photodamage. Antenna size heterogeneity showed little change under bleaching conditions with a significant increase in PSIIbeta only apparent in one species of coral. The thermostability of the oxygen evolving complex (OEC) and thylakoid membrane were found to increase during exposure to bleaching conditions and exceeded bleaching thresholds of corals. This rapid rise in temperature-dependent thermostability also occurred over seasons, where variation in ocean temperatures was matched by gradual shifts in OEC and thylakoid membrane thermotolerance. Variation in thermostability between species was not found to be linked to zooxanthellae genotype, and instead was related to the bleaching susceptibility of the host. Despite this capacity for resilience to bleaching conditions, the PSII reaction centres did not exhibit such a mechanism for rapid acclimatisation. Corals can only be as tolerant to bleaching conditions as their most sensitive component allows. The formation of nonfunctional PSII centres is therefore suggested to be involved in the initial photochemical damage to zooxanthellae which leads to a bleaching response. Zooxanthellae were found to be expelled irrespective of OEC function and thylakoid membrane integrity, as these sites of the photosynthetic apparatus were still intact when cells were collected from the water column. Although zooxanthellae were photosynthetically competent and morphologically intact upon expulsion, their longevity in the water column was dependent on the time of expulsion following the onset of bleaching and the ambient water temperatures. The survivorship of these zooxanthellae was restricted to a maximum of 5 days in the water column which suggests that unless expelled zooxanthellae inhabit other environs of coral reefs which may be more favourable for survival, their capacity for persistence in the environment is extremely limited. Chlorophyll a fluorescence measurements are a common tool for investigating photosynthetic impacts to in hospite zooxanthellae of corals. Pathways causing dark-reduction of the plastoquinone pool are shown to be active in corals and affect measurements which require dark-adaptation. Pre-exposure to far-red light was found to be an effective procedure to oxidise the inter-system electron transport chain and ensure determination of the true maximum quantum yield of PSII and accurate FICs. It is concluded that the trigger for coral bleaching lies in the photosynthetic apparatus of zooxanthellae and evidence is presented in support of this impact site not being the OEC or thylakoid membrane.
Savage, Anne Margaret. "Genetic diversity and photosynthetic characteristics of zooxanthellae (Symbiodinium)." Thesis, University of York, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.369298.
Повний текст джерелаToyoshima, Junko. "Cell migration of zooxanthellae in the coral Montipora capitata." Thesis, University of Hawaii at Manoa, 2003. http://hdl.handle.net/10125/7050.
Повний текст джерелаSquire, Louise R. "Natural variations in the zooxanthellae of temperate symbiotic Anthozoa." Thesis, Bangor University, 2000. https://research.bangor.ac.uk/portal/en/theses/natural-variations-in-the-zooxanthellae-of-temperate-symbiotic-anthozoa(a6342fd8-ff91-441e-85db-8b5b1c59167e).html.
Повний текст джерелаWang, Jih-Terng. "Nutritional interactions between the alga Symbiodinium and sea anemone Aiptasia pulchella." Thesis, University of York, 1998. http://etheses.whiterose.ac.uk/9750/.
Повний текст джерелаJokiel, Paul L. "The Photobiology of the Reef Coral Pocillopora damicornis and Symbiotic Zooxanthellae." Thesis, University of Hawai'i, Honolulu, 1985. http://hdl.handle.net/10125/15318.
Повний текст джерелаTypescript. Thesis (Ph.D.)--University of Hawaii, 1985. Bibliography: leaves 201-221.
Burghardt, Ingo. "Biology, diversity and evolution of 'solarpowered' Nudibranchia (Mollusca: Gastropoda) and their symbiosis with Zooxanthellae." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=983779732.
Повний текст джерелаHancock, Harmony Alise. "One Step Closer to Non-Invasive: Quantifying Coral Zooxanthellae Pigment Concentrations Using Bio-Optics." NSUWorks, 2012. http://nsuworks.nova.edu/occ_stuetd/189.
Повний текст джерелаVenn, Alexander Ashley. "Coral bleaching : the significance of the molecular diversity and photoprotective pigments of zooxanthellae (Symbiodinium)." Thesis, University of York, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.423605.
Повний текст джерелаGibbons, Christopher Lynton. "Carbon flux in the temperate zooxanthellate sea anemone Anthopleura aureoradiata : a thesis submitted to the Victoria University of Wellington in fulfilment of the requirements for the degree of Master of Science in Marine Biology /." ResearchArchive@Victoria e-Thesis, 2008. http://hdl.handle.net/10063/869.
Повний текст джерелаKrämer, Wiebke [Verfasser], Kai [Akademischer Betreuer] Bischof, and Claudio [Akademischer Betreuer] Richter. "Photoecophysiology of symbiotic zooxanthellae of hermatypic corals / Wiebke Krämer. Gutachter: Kai Bischof ; Claudio Richter. Betreuer: Kai Bischof." Bremen : Staats- und Universitätsbibliothek Bremen, 2012. http://d-nb.info/1071992791/34.
Повний текст джерелаRosset, Sabrina Laura. "Plasticity & adaptations of the coral-zooxanthellae symbiosis : responses to nutrient availability & insight into inherent thermal tolerance." Thesis, University of Southampton, 2016. https://eprints.soton.ac.uk/402319/.
Повний текст джерелаLien, Yi-Tiang. "Molecular phylogenetic and ecological analyses of algal endosymbionts (zooxanthellae) in the scleractinian corals in the temperate region of Japan." Kyoto University, 2013. http://hdl.handle.net/2433/175072.
Повний текст джерела0048
新制・課程博士
博士(農学)
甲第17643号
農博第2005号
新制||農||1012(附属図書館)
学位論文||H25||N4764(農学部図書室)
30409
京都大学大学院農学研究科応用生物科学専攻
(主査)教授 山下 洋, 教授 左子 芳彦, 教授 朝倉 彰
学位規則第4条第1項該当
Magalon, Hélène. "Dispersion du corail Pocillopora meandrina et de ses algues symbiotiques en Polynésie Française." Paris 6, 2005. http://www.theses.fr/2005PA066100.
Повний текст джерелаBonalume, Clauber. "Utilizaçao de tecnicas fotometricas para estudo do branqueamento e da quantidade de algas simbiontes em colonias coralinas." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/250453.
Повний текст джерелаDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Química
Made available in DSpace on 2018-08-09T00:07:10Z (GMT). No. of bitstreams: 1 Bonalume_Clauber_M.pdf: 1295510 bytes, checksum: 20c20d40e0b0a19af56f06a1076fb4cb (MD5) Previous issue date: 2006
Resumo: Corais marinhos do mundo inteiro estão ameaçados pelas alterações climáticas que vêm acontecendo nas duas últimas décadas. O aquecimento da superfície terrestre associado à intensificação do fenômeno El Niño têm causado prejuízos à saúde dos corais marinhos com intensidade, extensão e escala temporal sem precedentes. Quando expostos a situações de estresse ambiental, corais podem sofrer branqueamento, perdendo suas algas simbiontes, as zooxantelas, e morrer se não as recuperarem. Desta forma, métodos de avaliação da saúde dos corais tornam-se necessários. Dois métodos fotométricos foram desenvolvidos neste trabalho. No primeiro, utilizou-se a técnica de reflectância difusa para estudo do histórico de branqueamento de fatias de núcleos de esqueletos coralinos através da análise da cor do núcleo na direção do crescimento do coral. Esse método mostrou-se sensível à variação de cor ao longo da amostra e foi capaz de evidenciar o histórico conhecido de branqueamento de 2 entre 3 amostras analisadas. O método é promissor para o estudo de esqueletos, mas depende sensivelmente do tratamento prévio das amostras. No segundo, fotografaram-se amostras de Mussismilia híspida em seu ambiente natural na Laje de Santos. Foram colhidas amostras dos corais fotografados e foi determinada a densidade de zooxantelas em cada amostra. Utilizando o software MatLab e uma rotina específica de análise multivariada, estabeleceu-se uma correlação entre aspectos da imagem digital e a densidade de zooxantelas. A correlação obtida é boa, com erros menores que 35 % na estimativa da densidade de zooxantelas a partir das imagens digitais. O método é bastante promissor e tem a vantagem de ser não destrutivo.
Abstract: In the last two decades marine corals around the world have been threatened by weather global changes. Coral health is declining at unprecedented intensity, extension and temporal scale, by world surface heating associated with the strengthening of the El Niño Southern Oscillation. When exposed to environmental stresses coral can bleach, loosing their symbiotic algae, the zooxanthellae, and die if they are permanently lost. The development of methods capable to assess coral health is necessary. Two photometric methods were developed. In the first one, difuse reflectance spectrophotomety was used to analize the bleaching history of slices of coral skeletons by color analysis along the growing direction of the colony. The method showed apropriate sensibility for measuring color changes along the samples and was able to disclose the bleaching history in two of the three samples studied. However, results depend strongly on the previous treatment of the skeleton samples. In the second method, samples of Mussismilia híspida coral colonies were photographed in their natural habitat in the Laje de Santos marine conservation park. Small samples of the photographed coral were taken and the density of zooxanthellae in each sample was measured. Using the MatLab sofware and a specific algorithm for multivariated image analisys (MIA), a correlation between features of the images and the density of zooxanthellae was established. The correlation obtained is fairly good, with less than 35 % error in the estimation of zooxanthellae density from the digital images. The method is quite promissing and has the advantage of being nondestructive.
Mestrado
Físico-Química
Mestre em Química
Foster, Kristi A. "Effects of Reduced Light and Elevated Temperature on the Zooxanthellae Concentrations and Diameters, Pigment Concentrations, and Colony Color of Montastrea cavernosa." NSUWorks, 2005. http://nsuworks.nova.edu/occ_stuetd/275.
Повний текст джерелаZamani, Neviaty Putri. "Effects of environmental stress on cell division and other cellular parameters of zooxanthellae in the tropical symbiotic anemone Heteractis malu, Haddon and Shackleton." Thesis, University of Newcastle Upon Tyne, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294899.
Повний текст джерелаKennedy, Emma Victoria. "Climate change impacts on Caribbean coral reefs : reef accretion and scope for acclimation through symbiont genetic diversity." Thesis, University of Exeter, 2013. http://hdl.handle.net/10871/13142.
Повний текст джерелаRibeiro, Filipa Fonseca. "Optimization of heterotrophic feeding of the soft coral Sarcophyton cf. glaucum." Master's thesis, Universidade de Aveiro, 2015. http://hdl.handle.net/10773/16072.
Повний текст джерелаHeterotrophic feeding has an important role in the processes of growth and reproduction of mixotrophic corals. The soft coral Sarcophyton cf. glaucum is a good candidate for aquaculture due to its economic interest for the marine aquarium trade and for the bioprospection of marine natural products. The lack of information on heterotrophic feeding of this species with preserved microalgae conducted to development of this work. The present study aimed to evaluate the effect of the conservation processes of microalgae in its suitability as heterotrophic feeding for the mixotrophic coral S. cf. glaucum. Additionally, we aimed to identify the most suitable freeze-dried microalgae species and cell density to be employed in the culture of this mixotrophic coral species. Two experiments were performed: in the first experiment the microalgae Nannochloropsis oculata was supplied to coral fragments in three different preservation forms (live paste, frozen and freeze-dried) at the concentration of 106 cell mL-1; in the second experiment three different microalgae species (Nannochloropsis oculata, Isochrysis galbana and Phaeodactylum tricornutum) were tested in two different amounts: 7.33 mg L-1 (corresponding to the concentration of 106 cell mL-1 of Nannochloropsis oculata) and 3.66 mg L-1. Growth rate, survival, organic weight and photobiology of coral fragments, as well as water quality in culture tanks, were evaluated in both experiments. Preserved forms of microalgae did not demonstrated differences in growth rate, organic weight and survival rate of coral fragments, but affected water quality. Freeze-dried microalgae seems to be a good feed supply for coral aquaculture, as it has the best results and it has the higher shell-life time and the lower associated costs. Between the species evaluated in second experiment, Isochrysis galbana promoted higher specific growth rate and higher percentage of organic weight in the coral fragments; additionally the culture tanks supplied with this microalgae species also presented a better water quality in the end of the experiment.
A alimentação heterotrófica desempenha um papel importante nos processos de crescimento e reprodução dos corais mixotróficos. O coral mole Sarcophyton cf. glaucum é uma espécie com potencial para a aquacultura, devido ao seu interesse económico no comércio de organismos ornamentais e na bioprospecção de produtos naturais marinhos. A insuficiente informação sobre a alimentação heterotrófica desta espécie conduziu ao desenvolvimento deste estudo, que teve como objetivo avaliar o efeito dos processos de conservação de microalgas na sua adequabilidade como alimento heterotrófico para o coral mixotrófico S. cf. glaucum. Adicionalmente, pretendeu-se identificar qual a espécie de microalga mais adequada, assim como a quantidade mais apropriada a aplicar no cultivo desta espécie. Realizaram-se duas experiências: na primeira experiência, a microalga Nannochloropsis oculata foi fornecida como alimento a fragmentos de coral em três formas de preservação distintas (pasta de alga viva, alga congelada e alga liofilizada) na dosagem de 106 cell mL-1; na segunda experiência, foram testadas três espécies de microalga liofilizada (Nannochloropsis oculata, Isochrysis galbana e Phaeodactylum tricornutum) em duas dosagens diferentes: 7.33 mg L-1 (correspondente à concentração de 106 cell mL-1 de N. oculata) e 3.66 mg L-1. Em ambas as experiências foram avaliados os seguintes parâmetros: taxa de crescimento dos fragmentos de coral, sobrevivência, peso orgânico, fotobiologia e qualidade da água. Os resultados não mostraram diferenças na taxa de crescimento e peso orgânico, entre os fragmentos de coral alimentados com as três formas de preservação de microalga, no entanto, foram observadas diferenças na qualidade da água. A microalga liofilizada evidencia ser uma boa alternativa como alimento heterotrófico para a aquacultura de corais, uma vez que apresenta os melhores resultados nos parâmetros analisados, tem um tempo de prateleira maior e poucos custos associados ao seu armazenamento. Entre as espécies avaliadas na segunda experiência, a microalga I. galbana foi a que promoveu uma taxa de crescimento mais elevada e uma maior percentagem de peso orgânico nos fragmentos de coral. Adicionalmente, os tanques em que se forneceu I. galbana como alimento heterotrófico apresentaram um maior equilíbrio na qualidade de água de cultivo.
Wu, Yu Hui, and 吳鈺慧. "Heat shock proteins of coral and its symbiotic zooxanthellae." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/02241998699746796345.
Повний текст джерела國立中山大學
海洋資源學系
82
The coral community near a nuclear power plant of southern Taiwan has shown annual summer bleaching phenomenon due to heat stress by synthesizing highly conserved heat shock proteins ( hsp ). However, little is known of the biochemical responses concomitant with thermal bleaching of the coral. Our laboratory found that the coral cell of Acropora grandis and its symbiotic will synthesize 35 kDa protein in natural high seawater temper- ature. They synthesized hsp35 after heat shock, in coral cell this occurred at 29 ℃ while in zooxanthellae at 33 ℃. Hsp63 was also found in coral cell when 35S-methionine was added to label proteins for 2 hours after heat treatment for 1 hour at 35 ℃. Hsp35 sustained at least 13 hours in coral and zooxan- thellae at 35 ℃, and coral was bleached at this time. The coral tended to die and hsp35 disappeared when heat exposure lasted continuously for 22 hours at 35 ℃. This response was the same, even the coral exposured to 35 ℃ for only 3 hours. This indicated that coral is damaged when it temporally expo- sures to high temperature. If heat exposure was at 33 ℃, coral survived for at least 24 hours and hsp35 was not significant. The study shed light on a possibility of using hsp35 as an incicator of thermal bleaching in coral.
Buxton, LJ. "Assessing the photosynthetic impact of inorganic carbon in symbiotic dinoflagellates in culture and in coral host complexes." Thesis, 2008. http://hdl.handle.net/10453/29532.
Повний текст джерелаIncreased atmospheric carbon dioxide concentrations are causing warming of the Earth's atmosphere and having significant effects on the chemistry of the oceans. Disruption to seawater biochemistry is causing associated pH change known as ocean acidification and increased concentrations of dissolved inorganic carbon. Coral reefs are already at risk from increased seawater temperatures; however the impact of ocean acidification will be in addition to that of temperature change alone. The major objectives of this thesis were to provide insight into the effects of temperature, dissolved inorganic carbon concentration and seawater pH on the photosynthesis of the symbiotic algae Symbiodinium sp. both in vitro and in hospite. While it has been speculated that increased carbon dioxide may stimulate primary production, results presented here for short-term incubations show that Symbiodinium sp. photosynthesis is saturated at present concentrations (2 mM). However, photosynthesis in hospite can become significantly limited if DIC falls below 2 mM. This highlights the significant role of host inorganic carbon transport mechanisms for the maintenance in a healthy symbiosis. Symbiodinium sp. in culture however, show significant photosynthetic plasticity over a range of inorganic carbon concentrations (0.1 - 10 mM), and exhibit no photosynthetic inhibition at inorganic carbon concentrations well below ambient levels (0.4 - 2 mM). This suggests that Symbiodinium sp. have well developed carbon concentrating mechanisms and may be able to adapt to changes in inorganic carbon availability, whereas the same algae in hospite requires a higher DIC supply. Exposure to elevated temperature is known to cause photosynthetic inhibition in the coral symbiont Symbiodinium sp. Models of photophysiologcial thermal damage in corals can be generally divided into two broad groups; those that take place at the site of the light reactions and those that occur in the Calvin cycle. Results presented here have identified species- and cladal-specific heterogeneity in thermal inhibition of the dark reactions in the whole corals Stylophora pistillata and Pocillopora damicornis, and amongst Symbiodinium clades A, Band Cl. Furthermore, these patterns are not consistent when observed in vivo and in vitro. pH-drift experiments of Symbiodinium sp. grown in culture have identified significant cladal-specific pH ranges of clades A, B and subclade C1. Results show that Symbiodinium sp. are well adapted for living in rapidly changing pH and dissolved inorganic carbon environments. However, when measured in hospite in the C !- harbouring corals S. pistillata and P. damicornis, photosynthesis became significantly inhibited at pH 7.2 and 7.8 over short-term incubations. Furthermore, the thermal history of corals measured in the field had a significant effect on pH-susceptibility between summer and winter seasons. Results revealed an synergistic affect of elevated temperature and low pH on photosynthesis. The disparity between results observed in vivo and in vitro suggest that the cnidarian host may be considered the more vulnerable partner to changes in pH leading to photosynthetic inhibition and symbiotic dysfunction.
Schwarz, Jodi A. "Cellular and molecular aspects of cnidarian-algal associations." Thesis, 2002. http://hdl.handle.net/1957/32492.
Повний текст джерелаGraduation date: 2003
Tseng, Chih-Lin, and 曾智麟. "Variations in zooxanthellae and recovery of bleached colonies in Acropora intermedia." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/00163195368522569620.
Повний текст джерела國立中山大學
海洋生物研究所
93
The maximum quantum yield (Fv/Fm), zooxanthellae density, chlorophyll a concentration and protein concentration of non-bleached and bleached colonies of the reef coral Acropora intermedia were measured in inlet of The Third Nuclear Power Plant of Nanwan Bay in southern Taiwan. A significant positive correlation was found between Fv/Fm and chlorophyll a concentration per zooxanthellae of non-bleached colonies. The chlorophyll a concentration per zooxanthellae and zooxanthellae density of non-bleached colonies were lowest in summer, and were significantly negative correlated with total radiant heat and seawater temperature, respectively. This suggests that the seasonal variation exist, and they maybe regulated by seasonal fluctuation of radiation and temperature. The Fv/Fm, chlorophyll a concentration per cm-2, chlorophyll a concentration per zooxanthellae, zooxanthellae density and protein were significantly lower than those of the non-bleached colonies in the bleaching events. However, compared to the non-bleached colonies, zooxanthellae density, chlorophyll a concentration per cm-2 and protein of bleached colonies were increased and significantly higher than those of non-bleached colonies, then decreased to similar level. However, Fv/Fm increased to similar level, but chlorophyll a concentration per zooxanthellae remained lower. It suggests that number of zooxanthellae rapidly increased while remained stable chlorophyll a concentration during recovery.
Yang, Ya-Wen, and 楊雅雯. "Polymorphic symbiosis and phylogenetic analysis of zooxanthellae in the Indo- Pacific scleractinian corals." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/13624362212671261200.
Повний текст джерела國立中山大學
海洋生物研究所
89
Zooxanthellae are very important for the coral reef ecosystem. The diversity of coral hosts is high in the Indo-Pacific, but the diversity of zooxanthellae has not been broadly investigated. Southern Taiwan and Penghu Islands are coral reef and non-reefal communities, respectively. These localities were chosen as the sampling sites for this study to maximize the opportunity of surveying this region in the Indo-Pacific. Zooxanthellae diversity was investigated in 40 host species including 32 species of Scleractinia, 4 species of Actiniaria, 3 species of Milleporina and 1 species of Helioporacea using polymerase chain reaction (PCR) of the ssrRNA gene and restriction fragment length polymorphism (RFLP) patterns. The phylogenetic relationship of partial and complete sequences of the ssrRNA gene were also analysed. Aiptasia puchella harbors clade B; Oulastrea crispata only harbors clade E; while Acropora palifera and Montipora cactus harbor both clades C and E. Zooxanthellae isolated from all except the above 4 host species are identified as "clade C" sensu Rowan and Powers (1991a). Therefore, the clade C is the dominant type in the Indo-Pacific. Phylogenetic analyses based on partial and complete sequences obtained in this study and also from the GenBank data base demonstrate 4 clades (A, B, C and E) in the genus Symbiodinium. Clade E, classed as D3 RFLP type in previous studies, is a distinct clade differing from A, B and C by RFLP and sequencing data. Clade E has only been found in Scleractinia host species collected in shallow-water habitats in the Pacific. The composition of zooxanthellae clades and ecological pattern of polymorphic symbiosis is not consistent with the irradiance adaptation hypothesis in the Caribbean. A literature survey of zooxanthellae in Scleractinian hosts indicates a significant difference between the Caribbean and the Pacific. The documented biogeography of zooxanthellae clades and the ecological pattern of polymorphic symbiosis are also differ between the Caribbean and the Indo-Pacific.
SHUN, WEI-QIAN, and 孫維謙. "Preparation of DNA from scleractinian coral and zooxanthella and used for DNA hybridization." Thesis, 1992. http://ndltd.ncl.edu.tw/handle/53034627866790383600.
Повний текст джерелаCheng, Ying-Min, and 鄭英敏. "Intracellular Survival Mechanisms of Zooxanthellae in Cnidarian Digestive Cells—The Critical Role of ApRab5 and ApRab7." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/75853266093293634333.
Повний текст джерела國立中山大學
海洋生物研究所
92
Marine cnidarian-microalgal endosymbiosis is an ecologically important intracellular association. However, its underlying molecular mechanisms are essentially unknown. In light of the critical roles of host phagocytosis in intracellular fates of a variety of microbes, and the Rab small GTPases as key mediators of host-symbiont interaction, we set out to investigate the potential involvement of Aiptasia Rab proteins in the model photosynthetic endosymbiosis between the sea anemone, Aiptasia pulchella and the symbiotic dinoflagellate (commonly called zooxanthellae), Symbiodinium spp. Many Aiptasia Rab homologue-encoding cDNA fragments were first cloned through our degenerate RT-PCR and RACE reactions. Significantly, Aiptasia homologues of Rab5 and Rab7 (ApRab5 and ApRab7), two Rabs known to be critical regulators of phagosome maturation were also identified in the screen. The overall sequence identities of ApRab5 and ApRab7 to those of human Rab5C and Rab7 were very extensive, and EGFP reporter, protein fractionation, and immuno-fluorescence studies all suggested that the similarity of the Aiptasia Rabs to their human counterparts extended to the functional levels. Finally, although the phagosomes enclosing latex beads stained positive for ApRab5 and ApRab7 with kinetics characteristics of normal phagosomal maturation, the phagosomes housing zooxanthellae only stained positive for ApRab5. Furthermore, the association of ApRab5 with and the exclusion of ApRab7 from the zooxanthellae-containing phagosomes could be reversed by the heat-killed or photosynthesis-impaired symbionts. Overall, our present study has identified ApRab5 and ApRab7 as potential key regulators of the Aiptasia-Symbiodinium endosymbiosis
Jiang, Hie, and 姜海. "The Effects of Different Nitrogenous Sources to the Sea Anemone Aiptasia Pulchella and Its Symbiotic Zooxanthellae." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/16850892768706313993.
Повний текст джерела國立中山大學
海洋生物學系
85
The aims of this study are to understand the role of algal symbiosis and translocation regulation of symbiotic sea anemone Aiptasia pulchella through different nitrogenous sources. The effects of different nitrogenous treatments on symbiotic interaction were investigated by the carbon and nitrogen stable isotope composition of sea anemone tissue and zooxanthellae. Colonies of the sea anemones were fed with newly hatched Artemia nauplii and/or a fish feed or raised in the culture water with or without enrichment of ammonium every other day. The result shows that there was no significant difference in δ13C of zooxanthellae among treatments, indicating that the carbon source of the zooxanthellae is independent of nitrogenous source and is dependent of photosynthesis. The δ13C and δ15N values of sea anemone tissue and zooxanthellae indicate that the addition of ammonium caused a decrease in translocation of nitrogenous matters and photosynthetic products from zooxanthellae to the host, and more over; the input of ammonium caused a tendency toward heterotrophy for the zooxanthellae.
Michalek-Wagner, Kirsten. "The chemical ecology of the soft coral-zooxanthellae association and its signficance to the bleaching process." Thesis, 1999. https://researchonline.jcu.edu.au/27503/1/27503-michalek-wagner-1999-thesis.pdf.
Повний текст джерела"Seasonal change in the chlorophyll content, density and types of symbiotic algae in Hong Kong corals." 2010. http://library.cuhk.edu.hk/record=b5894274.
Повний текст джерела"November 2009."
Thesis (M.Phil.)--Chinese University of Hong Kong, 2010.
Includes bibliographical references (leaves 268-277).
Abstracts in English and Chinese.
Acknowledgements --- p.i
Abstract --- p.iii
Content --- p.vii
List of Tables --- p.ix
List of Figures --- p.xx
Chapter Chapter One --- General Introduction --- p.1
Introduction --- p.1
Coral Reefs --- p.1
Coral-Algal Symbiosis --- p.2
The Dinoflagellate Symbiodinium --- p.11
Coral Communities in Hong Kong --- p.18
Objectives --- p.21
Study Sites - Tung Ping Chau Marine Park --- p.21
Coral Species chosen for the Experiment --- p.23
Platygyra acuta --- p.23
Porites lutea --- p.23
Thesis Outline --- p.24
Chapter Chapter Two --- Temporal variation in photophysiological parameters of Hong Kong corals and their relationship with the environmental factors --- p.28
Introduction --- p.28
Methods and Materials --- p.33
Study Sites --- p.33
Coral Sampling for zooxanthellae density and chlorophyll concentration analysis --- p.34
Measurement of changes in the photosynthtic activity of the corals --- p.38
Environmental Factors --- p.38
Data analysis --- p.39
Results --- p.41
Density of Zooxanthellae --- p.41
Concentrations of Chlorophyll a and c2 (per zooxanthella) --- p.43
Concentrations of Chlorophyll a and c2 (per surface area) --- p.46
Chlorophyll ratio --- p.49
Effective quantum yield --- p.51
Photosynthetically active radiation (PAR) --- p.54
Intra-colony variation for Platygyra acuta --- p.56
Comparison between Platygyra acuta and Porites lutea --- p.64
Environmental factors and their correlations with photophysiological parameters in corals --- p.68
Discussion --- p.73
Density of zooxanthellae --- p.74
Concentrations of Chlorophyll a and c2 --- p.80
Chlorophyll ratio --- p.85
Effective quantum yield --- p.88
Intra-colony variation --- p.94
Between species comparison --- p.98
Summary --- p.107
Chapter Chapter Three --- PCR-RFLP Analysis on Symbiodinium in Platygyra acuta --- p.241
Introduction --- p.241
Methods and Materials --- p.244
Sample collection --- p.244
Molecular phylotyping --- p.245
Results --- p.247
Discussion --- p.249
Summary --- p.257
Chapter Chapter Four --- Summary and Conclusion --- p.262
References --- p.268
Burghardt, Ingo [Verfasser]. "Biology, diversity and evolution of 'solarpowered' Nudibranchia (Mollusca: Gastropoda) and their symbiosis with Zooxanthellae / vorgelegt von Ingo Burghardt." 2006. http://d-nb.info/983779732/34.
Повний текст джерелаLEE, JIN-JIE, and 李進傑. "The Effect of pH and Culture Medium on the Growth of Symbiotic Zooxanthellae Isolated from Taiwan Common Cnidarian." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/9vy9jt.
Повний текст джерела國立高雄海洋科技大學
水產養殖研究所
106
The cnidarians form the amazing coral reef ecosystem mutually with zooxanthellae, its foundation was based on the intracellular symbiosis. By endosymbiosis, zooxanthellae were capable of living in cnidarian host gastrodermal endoderm cells with large numbers. Traditionally, the pH of symbiosomal lumen was thought to be 6-7. However, a recently report by utilizing pH indicators indicated that the pH of symbiosomal lumen in coral cells is below 4, revealing that the regulatory mechanism of symbiosome acidification should be clarified. Therefore, this study hypothesized that the pH of symbiosomal lumen in different cnidarian were different. Through culturing the fresh-isolated zooxanthellae with the different medium, the most suitable pH range of different zooxanthellae were evaluated. In this study, a total of eight of cnidarians species, and one test bivalves. The current results indicate that the zooxanthellae from Aiptasia pulchella, Bolocera mcmurrichi, Fungiidae spp. and Sinularia polydactyla, which were can growth at 6.2 and 8.6. The immunofluorescence showed that V-ATPase D was not abundantly distributed on the symbiosomal membrane of coral cells, suggesting symbiosomes were not acidic vesicles. Keyword:Cnidarians、Symbiosome、Zooxanthellae、pH
Kampmann, Heike [Verfasser]. "Photobiologische, energetische und genetische Aspekte des mutualistischen Zusammenlebens von Zooxanthellen (Symbiodinium sp.) und Steinkorallen im Golf von Aqaba, Jordanien / vorgelegt von Heike Kampmann." 2003. http://d-nb.info/969446098/34.
Повний текст джерелаSong, Po-Ching, and 宋柏青. "Study on the iron-harvesting pathway of host-harbored zooxanthellae and the high temperature induces Symbiodinium iron-deficiency genes expression during Aiptasia-Symbiodinium endosymbiosis." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/98501562492808508766.
Повний текст джерела國立中山大學
海洋科學系研究所
104
Coral bleaching is the consequence of disruption of the mutualistic Cnidaria-dinoflagellate association. Elevated seawater temperatures have been proposed as the most likely cause of coral bleaching whose severity is enhanced by a limitation in the bioavailability of iron. Iron is required by numerous organisms including the zooxanthellae residing inside the symbiosome of cnidarian cells. However, the knowledge of how symbiotic zooxanthellae obtain iron from the host cells and how elevated water temperature affects the association is very limited. Since cellular iron acquisition is known to be mediated through transferrin receptor-mediated endocytosis, a vesicular trafficking pathway specifically regulated by Rab4 and Rab5, we set out to examine the roles of these key proteins in the iron acquisition by the symbiotic Symbiodinium. Thus, we hypothesized that the iron recruitments into symbiotic zooxanthellae-housed symbiosomes may be dependent on rab4/rab5-mediated fusion with vesicles containing iron-bound transferrins and will be retarded under elevated temperature. In this study, we cloned a novel monolobal transferrin (ApTF) gene from the tropical sea anemone Aiptasia pulchella and confirmed that the association of ApTF with A. pulchella Rab4 (ApRab4) or A. pulchella Rab5 (ApRab5) vesicles is inhibited by elevated temperature through immunofluorescence analysis. We confirmed the iron-deficient phenomenon by demonstrating the induced overexpression of iron-deficiency-responsive genes, flavodoxin and high-affinity iron permease 1, and reduced intracellular iron concentration in zooxanthellae under desferrioxamine B (iron chelator) and high temperature treatment. In conclusion, our data are consistent with algal iron deficiency being a contributing factor for the thermal stress-induced bleaching of symbiotic cnidarians.
Boutilier, Ryan Michael. "Aspects of Nitrogen Metabolism in Symbiotic Cnidarians." Thesis, 2012. http://hdl.handle.net/10214/3874.
Повний текст джерелаMann, Timothy. "Investigation of the effect of GFP-type proteins on photoinduced proton flux in reef corals." Thesis, 2017. http://hdl.handle.net/1959.7/uws:51204.
Повний текст джерела