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Статті в журналах з теми "ZNF92"
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Bhattacharya, Udayan, Mohammad Kamran, Maroua Manai, Massimo Cristofanilli, and Tan A. Ince. "Cell-of-Origin Targeted Drug Repurposing for Triple-Negative and Inflammatory Breast Carcinoma with HDAC and HSP90 Inhibitors Combined with Niclosamide." Cancers 15, no. 2 (January 4, 2023): 332. http://dx.doi.org/10.3390/cancers15020332.
Повний текст джерелаАнотація:
We recently identified a cell-of-origin-specific mRNA signature associated with metastasis and poor outcome in triple-negative carcinoma (TNBC). This TNBC cell-of-origin signature is associated with the over-expression of histone deacetylases and zinc finger protein HDAC1, HDAC7, and ZNF92, respectively. Based on this signature, we discovered that the combination of three drugs (an HDAC inhibitor, an anti-helminthic Niclosamide, and an antibiotic Tanespimycin that inhibits HSP90) synergistically reduces the proliferation of the twelve tested TNBC cell lines. Additionally, we discovered that four out of five inflammatory breast carcinoma cell lines are sensitive to this combination. Significantly, the concentration of the drugs that are used in these experiments are within or below clinically achievable dose, and the synergistic activity only emerged when all three drugs were combined. Our results suggest that HDAC and HSP90 inhibitors combined with the tapeworm drug Niclosamide can achieve remarkably synergistic inhibition of TNBC and IBC. Since Niclosamide, HDAC, and HSP90 inhibitors were approved for clinical use for other cancer types, it may be possible to repurpose their combination for TNBC and IBC.
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Wang, Luqun, Qiong Liu, Hao Li, Li Lizhen, and Xin Wang. "The Establishment of Bortezomib Resistant Myeloma Cell Line KM3/BTZ and Explore the Resistance Mechanism." Blood 124, no. 21 (December 6, 2014): 5226. http://dx.doi.org/10.1182/blood.v124.21.5226.5226.
Повний текст джерелаАнотація:
Abstract Background: Multiple myeloma (MM) is an incurable B-cell malignancy resulting in significant morbidity and mortality, the incidence of second place in hematological malignancies. The proteasome inhibitor bortezomib inhibits IkappaB degradation, prevents NF-kappaB activation, and induces apoptosis in MM cells, has become first-line drug to MM. Despite its promising activity in traditional chemoresistant myeloma patients, however, some patients are resistant or become refractory to bortezomib. Chauhan D et al reported that Blockade of Hsp27 overcomes bortezomib resistance in lymphoma cells (Chauhan D et al., Cancer Re, 2003). However, bortezomib resistance mechanisms in MM remain controversial, the molecular basis of this reduced responsiveness is currently not fully understood. Objective: To establish a bortezomib-resistant cell line KM3 /BTZ of MM, then search for genes related to bortezomib resistance phenotype by analyzing the differential gene expression patterns with cDNA microarray,and explain the mechanism of bortezomib-resistant multiple myeloma. Methods: Bortezomib-resistant KM3/BTZ cell line was generated by increasing concentration of bortezomib to induce KM3 cells in vitro. MTT assay was employed to detect the cytotoxic effect on KM3 and KM3/BTZ cells. The cells apoptosis were analyzed by flow cytometry. We detect the gene expression profile changes between KM3/BTZ and its parent cell line KM3 cells by CDNA microarray. Combining of molecular annotation system MAS3.0 software and detailed analysis of documented resistance genes were used to analyze the data. Specific differentially-expressed genes were chosen for further verification using real-time RT-PCR. The expression of multi-drug resistance 1(MDR-1) gene mRNA was determined by RT-PCR. Results: The KM3/BTZ cell line was established successfully. Bortezomib resistance index values were significantly higher in KM3/BTZ (IC50 =351.2±3.51ng /ml) when compared to KM3 cells (IC50 = 17.8±1.03ng /ml),and the resistance index is 19.7 (P< 0.05). Through gene expression profiling filtering, compared with KM3,770 significantly differently expressed genes were screened out in the KM3/BTZ cell line, of which up and down regulated genes were 287 and 383 respectively, in which heat shock protein family gene HSPB2 was significantly up-regulated (Ratio=14.7455). Downregulated gene list in KM3/BTZ cell line included transcriptional regulators like ZNF family proteins (Tab 1). Upregulated genes in KM3/BTZ cell line included signal transduction related genes like MS4A family protein (Tab 1) also transcriptional regulators like ZNF family proteins (Tab 1). The expression trend of eight genes for further verification was almost consistent with the microarray, excepting gene of JUN (Fig 1). The expression of MDR-1 mRNA was not observed in either resistant or parental cells (Fig 2). Conclusion: We have successfully established bortezomib-resistant cell line KM3/BTZ. MDR-1 was not involved in bortezomib-resistant multiple myeloma cells.HSPB2, ZNF and MS4A family genes are probably related to bortezomib resistance in KM3/BTZ cells. Our results suggest that they may be new targets to overcome resistance to bortezomib in patients with MM. Table 1. ZNF and MS4A family gene expression in KM3/BTZ and KM3 cells Gene B signal* B detection* A signal* A detection* BvsA signal log ratio* BvsA change* ZNF711 85.08 P 206.16 P 0.41269 D ZNF92 6.75 P 14.57 P 0.46328 D ZNF704 10.7 A 26.78 P 0.39955 D ZNF492 7.08 P 3.48 P 2.03448 I ZNF532 87.93 P 43.54 A 2.01952 I ZNF594 10.25 P 4.63 P 2.21382 I ZNF789 269.79 P 97.3 P 2.77276 I ZNF506 330.36 P 149.48 P 2.21006 I MS4A3 1721.58 P 145.3 P 11.84845 I MS4A4A 2688.23 P 100.8 P 26.66895 I MS4A6A 141.96 P 56.47 M 2.513901 I MS4A1 49.02 P 53.11 P 0.92299 NC P: High expression; A: Low expression; M:Marginally D: Decrease; I: Increase; NC: No change *: A:KM3; B: KM3/BTZ Figure 1 Figure 1. Correlations of gene expression changes between oligonueleotide Microarray and qRT-PCR, showing change of selected gene in KM3/BTZ compared with KM3.The Y-axis indicates the log2 transformed ratio of mRNA expression. Figure 2 Figure 2. The expression level of MDR-1 mRNA in KM3 and KM3/BTZ and K562/A cell lines. M: DNA Marker; 1-2: K562/A cell line; 3-4: KM3cell line; 5-6: KM3/BTZ cell line Disclosures No relevant conflicts of interest to declare.
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Lee, Il-Kwon, Jeong-Hwa Choi, Yu Ra Lee, Yeo-Kyeoung Kim, Hee Nam Kim, Kyeong-Soo Park, Je-Jung Lee, et al. "A Single Nucleotide Polymorphism and Its Haplotype of ZNF42 Transcriptional Modulator Gene Predisposes Individuals to High-Risk Acute Myelogenous Leukemia (AML)." Blood 106, no. 11 (November 16, 2005): 4248. http://dx.doi.org/10.1182/blood.v106.11.4248.4248.
Повний текст джерелаАнотація:
Abstract ZNF42 (MZF-1 or Human Zinc finger protein 42) is a hematopoietic transcription factor regulating the differentiation and proliferation of myeloid cells. ZNF42 belongs to the Krupple-class zinc finger protein, which is expressed in myeloid progenitor cells. As a transcriptional regulator it has been found to be pivotal in hematopoietic development, especially in granulopoiesis. ZNF42 is a bi-functional transcriptional regulator, by repressing transcription in non-hematopoietic cells, but trans-activating hematopoiesis-related genes. Despite the important role of ZNF42 in differentiation and proliferation in the myeloid lineage cells, molecular epidemiologic studies of ZNF42 have not been conducted. In this case-control study, we conducted a comprehensive analysis of the genomic region of ZNF42, including SNP discovery by re-sequencing of the promoter and exon-encompassing regions, haplotype construction and construction of linkage disequilibrium (LD) map and comparison LD structure among four different populations data from the International HapMap project. In total, 275 de novo AML patients plus age- and sex-matched controls were recruited and four coding non-synonymous SNPs were genotyped by Pyrosequencing for this study. All genotypes frequencies were in Hardy-Weinberg equilibrium. A non-synonymous SNP (G/A altering amino acid R51H) revealed strong association with increased susceptibility to AML. Relative to individuals with the GG genotype, those with the A allele (AA + AG) had a 4.8-fold (95% CI, 3.3–7.0) (p=4.111e-17) risk of development of AML. Also four common (> 5% frequency, cumulative frequency of over 96%) haplotypes were identified and the frequencies of the common haplotypes predicted were similar between cases and controls. When we studied if there is haplotype-based association to examine the contribution of common genetic variation at the ZNF42 locus to AML risk among Korean, two haplotypes (G-G and A-G from R51H-R130Q) showed statistically significant association.. These results suggest that ZNF42 variants and its haplotypes are significantly associated with increased susceptibility to AML.
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Hou, Zhao Xia, Zhao Lu Xue, Shao Hong Wang, Xiao Dan Hu, Hao Ran Lu, Chang Lei Niu, Hao Wang, Cai Wang, and Yin Zhou. "Thermal Stability and Structure of Tellurite Glass." Key Engineering Materials 512-515 (June 2012): 994–97. http://dx.doi.org/10.4028/www.scientific.net/kem.512-515.994.
Повний текст джерелаАнотація:
Oxyfluoride tellurite glass with the composition of TeO2-AlF3-LaF3-ZnO/ZnCl2/ZnF2 was prepared successfully. The thermal stability and structure of TeO2-AlF3-LaF3 system tellurite glass were studied bySubscript text DSC and IR spectra. The results indicated that glass transition temperature of TeO2-AlF3-LaF3-ZnO/ZnCl2/ZnF2 glass was higher than that of (1-x)TeO2-xAlF3 (x=10%, 20%, 30%, 40%, 50%, in mol%) binary glass system slightly. A small number of ZnF2/ZnCl2/ZnO (5mol%) improved glass thermal stability. After adding 5mol% ZnF2/ZnO/ZnCl2 into fluoride tellurite glass respectively, glass transition temperature increased in turn. The introduction of 5mol% different zinc compounds had a little impact on the glass network structure.
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Elsayed, E. Mostafa, Mohamed M. Rashad, H. F. Y. Khalil, M. R. Hussein, M. M. B. El-Sabbah, and I. A. Ibrahim. "Electrochemical Performance of Nanocrystalline Zinc Ferrite Films Synthesized Using Electrodeposition." Key Engineering Materials 835 (March 2020): 1–6. http://dx.doi.org/10.4028/www.scientific.net/kem.835.1.
Повний текст джерелаАнотація:
Nanocrystalline spinel zinc ferrite ZnFe2O4 thin film has been studied and synthesized via the electrodeposition-anodization process. Electrodeposited ZnFe2 alloys were obtained from aqueous sulphate bath. The resulted alloys were electrochemically oxidized in strong alkaline solution (1 M KOH) at room temperature to the analogous hydroxides. The electroanodized ZnFe2 alloy film was annealed in air at 400 °C for 2 h to get the required zinc ferrite. The electrochemical factors controlling of the electrodeposition of ZnFe2 alloys such as the bath temperature, agitation, the current density were studied and optimized. The crystal structure, crystal size and microstructure of the produced ferrites were investigated using X-ray diffraction (XRD) and scanning electron microscopy (SEM). The deposited film was mainly composed of ZnFe2O4 based on XRD studies. The produced film had a spinel structure and the crystallite size was 4.9 nm. SEM micrograph of the resulted zinc ferrite particles shows compact crystallites shapes and agglomerated chains with smallest semicircular particles like morphology.
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Zhang, Ye, and Hong Bing Ji. "Preparation of ZnF2 Nano-Particles by Electrochemical Anodization Method." Advanced Materials Research 476-478 (February 2012): 1616–20. http://dx.doi.org/10.4028/www.scientific.net/amr.476-478.1616.
Повний текст джерелаАнотація:
ZnF2 nano-particles were prepared by electrochemical anodization method at room temperature. Zn foil acted as anode and Pb sheet served as cathode, and the complex solution of NH4F-H2O2-C2H5OH was used as electrolyte. The morphology, composition and crystalline structure of the as-prepared product were characterized by SEM, EDS and XRD techniques. The results showed that the product was rhombic nano-particles with the length of 50-200nm and the width of 50-100nm, the crystalline structure of the product was ZnF2 and ZnO, and the ZnF2 was the dominant component. Effects of NH4F concentration, applied voltage and anodization time on the morphology of the product were investigated.
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Maletin, M., E. G. Moshopoulou, and V. V. Srdic. "Magnetic properties of ZnFe2 O4 and In-doped ZnFe2 O4 nanoparticles." physica status solidi (a) 205, no. 8 (May 26, 2008): 1831–34. http://dx.doi.org/10.1002/pssa.200723633.
Повний текст джерела
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Richter, Theresia M. M., Sylvain LeTonquesse, Nicolas S. A. Alt, Eberhard Schlücker, and Rainer Niewa. "Trigonal-Bipyramidal Coordination in First Ammoniates of ZnF2: ZnF2(NH3)3 and ZnF2(NH3)2." Inorganic Chemistry 55, no. 5 (February 19, 2016): 2488–98. http://dx.doi.org/10.1021/acs.inorgchem.5b02837.
Повний текст джерела
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Asseid, Fathi M., Jack M. Miller, and James H. Clark. "FT-IR and 29Si, 27Al, and 19F MAS NMR studies of the adsorption of CdF2, ZnF2, and CuF2 onto montmorillonite K10; activity towards Friedel–Crafts alkylation." Canadian Journal of Chemistry 70, no. 9 (September 1, 1992): 2398–404. http://dx.doi.org/10.1139/v92-304.
Повний текст джерелаАнотація:
ZnF2, CdF2, and CuF2 have been adsorbed onto the surface of montmorillonite K10, and the infrared and 19F, 27Al, and 29Si MAS NMR spectra of the resulting reagents over a range of loadings and activation temperatures have been obtained. CuF2 was observed to attack the SiO2 layer and form the complex CuSiF6, ZnF2 tends to attack the aluminium oxide layer, in which Zn isomorphously replaces Al, and forms AlF3 and AlF4− complexes. The spectroscopic evidence rules out the formation of any Al–F and (or) Si–F species as CdF2 is adsorbed on the surface of montmorillonite K10. The reactivity of MF2–K10 reagents towards the Friedel–Crafts reaction of benzene with benzyl chloride varies from one reagent to another. ZnF2–K10 was observed to be the most reactive and CuF2 was the least reactive.
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Lv, Hao, Yao Ming Ding, Ai Mei Liu, Ju Fang Tong, Xu Nong Yi, and Qian Guang Li. "Nucleation Agent Choice of Li2O–Al2O3–SiO2 Glass Ceramic Having Ideal Micro Spherical Crystal Grains." Advanced Materials Research 311-313 (August 2011): 1332–35. http://dx.doi.org/10.4028/www.scientific.net/amr.311-313.1332.
Повний текст джерелаАнотація:
The nucleation and crystallization of Li2O-Al2O3-SiO2(LAS) glass ceramic with one(P2O5, TiO2, ZnF2) and two nucleating agents(P2O5+TiO2, P2O5+ZnF2) are investigated by the differential thermal analysis(DTA)，X-ray diffraction(XRD) and the scanning electron microscopy(SEM). Research results show that the LAS glass ceramic with nucleating agent of P2O5can obtain ideal micro spherical crystal grains and the crystallization behavior can be better controlled.
Дисертації з теми "ZNF92"
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Trusso, Maria Allegra. "THE GENETICS OF BIPOLAR DISORDER AND THE ROLE OF HETEROZYGOSITY FOR NEURONAL CEROID LIPOFUSCINOSIS." Doctoral thesis, Università di Siena, 2022. http://hdl.handle.net/11365/1214195.
Повний текст джерелаАнотація:
Introduction. Bipolar Disorder (BD) is an heritable chronic mental disorder causing psychosocial
impairment, affecting patients with depressive/manic episodes. The familial transmission of BD does not
follow any of the simple Mendelian patterns of inheritance, demonstrating the involvement of multiple
susceptibility genes.
Materials and Method. Whole Exome Sequencing (WES) was performed in eight subjects of a large family
counting twelve BD affected people. We selected variants in common between the affected subjects, once
including and once excluding a “borderline” subject with moderate anxiety and traits of obsessive-
compulsive disorder.
Results. Results were in favour of a Digenic model of transmission, with a heterozygous missense variant in
CLN6 resulting in a “borderline” phenotype that if combined with a heterozygous missense variant in ZNF92
is responsible for the more severe BD phenotype. Both rare missense changes are predicted to disrupt the
protein function.
Conclusions. Loss of both alleles in CLN6 causes Neuronal Ceroid Lipofuscinosis, a severe progressive
neurological disorder of childhood. Our results indicate that heterozygous CLN6 carriers, previously reported
as healthy, may be susceptible to bipolar disorder late in life. Additional variants, such as that in ZNF92
reported here, may further worsen the phenotype in a setting of digenic disorder. Further investigation on a
larger cohort should be performed in order to better characterize the contribution of each gene.
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PANICO, MARIA BEATRICE. "Espressione e localizzazione subcellulare della Zinc Finger Protein 9, prodotto del gene della distrofia miotonica tipo 2." Doctoral thesis, Università degli Studi di Roma "Tor Vergata", 2008. http://hdl.handle.net/2108/413.
Повний текст джерелаАнотація:
Espressione e localizzazione subcellulare della Zinc Finger Protein 9, prodotto del gene della distrofia miotonica tipo 2
Dr. M. B. Panico
Il difetto genetico alla base della distrofia miotonica di tipo 2 (DM2) è una espansione CCTG localizzata nell’introne 1 della zinc finger protein 9 (ZNF9) (cromosoma 3q21). ZNF9 è una proteina di 19kDalton di peso molecolare, altamente conservata ed espressa in numerosi tessuti, ma la sua localizzazione cellulare e la sua funzione sono ancora sconosciute. Pertanto, abbiamo utilizzato un anticorpo policlonale precedentemente caratterizzato per studiare attraverso esperimenti di immunofluorescenza (IF) la localizzazione subcellulare di ZNF9 nel tessuto muscolare normale umano e di ratto. In sezioni muscolari longitudinali, l’anticorpo per ZNF9 mostrava un pattern di bandeggiatura trasversale regolare di 1-2 μm di spessore che si estendeva per tutta la lunghezza della fibra. In esperimenti di doppia IF osservata al microscopio confocale, ZNF9 presentava una colocalizzazione con proteine localizzate a livello della giunzione I-Z come l’ATPasi del reticolo sarcoplasmatico Ca/Mg, la regione T12 della titina e la desmina. Inoltre, in sezioni muscolari di pazienti con DM2 la distribuzione intracellulare di ZNF9 risultava paragonabile a quella dei controlli. Questi dati indicano che la proteina ZNF9 è espressa ad un livello elevato nelle miofibrille normali e la sua distribuzione cellulare non è alterata nella DM2.Expression and subcellular localization of myotonic dystrophy type 2 protein ZNF9 Dr. M.B.Panico The genetic defect underlying myotonic dystrophy type 2 (DM2) is a CCTG expansion located in intron 1 of the zinc finger protein 9 (ZNF9) gene in chromosome 3q21. ZNF9 is a 19kDa highly conserved protein expressed in various tissues, but its cellular localization and function are still unclear. We have therefore used a previously characterized polyclonal antibody to detect by immunofluorescence (IF) the subcellular localization of ZNF9 in normal human and rat skeletal muscle. In longitudinally sectioned myofibers, IF reactivity for ZNF9 appeared as regular transverse bands 1-1.2 μm thick, throughout the fiber width. In double IF experiments observed by confocal microscopy, ZNF9 showed co-localization with proteins localized at the I-Z band junction such as the sarcoplasmic reticulum Ca/Mg ATPase, the T12 region of titin and desmin. Moreover, in DM2 muscle the ZNF9 intracellular distribution was comparable to that of control. These data indicate that ZNF9 is highly expressed in normal myofibers and its cellular distribution is apparently not disrupted in DM2.
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Torre, Eliana do Nascimento. "Metaloproteinases da matriz extracelular e cárie dentária: avaliação dos fluoretos TiF4, SnF2, ZnF2 e NaF na expressão das MMP-2 e MMP-9 salivares humanas." Universidade Federal de Pelotas, 2014. http://repositorio.ufpel.edu.br:8080/handle/prefix/3468.
Повний текст джерелаАнотація:
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Enzimas bacterianas foram consideradas as principais responsáveis pela degradação da matriz dentinária durante o processo de lesão de cárie dentária. No entanto, a literatura emergente sugere que enzimas derivadas do hospedeiro, e, em particular, as metaloproteinases da matriz (MMPs) contidas na dentina e na saliva podem desempenhar um papel importante neste processo, pela sua capacidade em degradar a matriz orgânica dentinária. Estes fatos possibilitam o estudo de novas opções terapêuticas para a prevenção e tratamento da lesão de cárie dentária. Uma revisão de literatura foi feita, como primeira parte desta tese, para elucidar a presença, localização e o nível de atividade das principais MMPs encontradas no tecido cariado humano, abordando temas relevantes, sobre o papel local das MMPs em relação a este tecido. A seleção dos trabalhos foi feita na base de dados Pubmed, em artigos com idioma inglês, até novembro de 2014. De 411 artigos elegíveis, 14 foram selecionados para o estudo completo, e 08 foram incluídos nesta revisão. A partir desta análise, respeitando os critérios de inclusão/exclusão propostos, pode-se concluir que a literatura possui poucos dados sobre a presença, localização, e nível de atividade enzimática no tecido cariado humano. Além disto, foi encontrada também uma heterogeneidade grande, a respeito das metodologias utilizadas para alcançar os objetivos de cada trabalho, e em relação à classificação do tipo de tecido dentinário cariado estudado. As MMPs mais frequentemente estudadas na dentina cariada humana foram, a MMP-2 e a MMP-9, seguidas pelas MMP-8 e MMP-20. Contudo pode-se sugerir que a MMP-2 parece ser, dentre as metaloproteinases estudadas, a que existe em maior quantidade no tecido dentinário cariado, localizada principalmente nos túbulos dentinários da dentina afetada (interna), próximo ao tecido pulpar. Como segunda parte, foi avaliada a possibilidade de utilização de alguns fluoretos como inibidores de MMPs salivares humanas, para interferir na prevenção ou progressão de lesões de cárie. Os fluoretos de estanho (SnF2), fluoreto de zinco (ZnF2) e o tetrafluoreto de titânio (TiF4), foram testados em comparação com o fluoreto de sódio (NaF), para a inibição de MMPs salivares. Saliva de doadores saudáveis foi usada em ensaio por zimografia e testadas as soluções em concentrações clínicas relevantes. A zimografia mostrou inibição das MMP-2 e MMP-9 em ordem decrescente para os fluoretos ZnF2e TiF4> NaF e SnF2. Com as limitações de um estudo in vitro, pode-se sugerir que devido aos fluoretos testados terem mostrado capacidade de inibição de MMP-2 e -9 salivares,uma nova visão e perspectiva se abre, para o estudo destes materiais, na prevenção das lesões de cárie dentária.
Bacterial enzymes were considered the main responsible factor for the degradation of the dentine matrix during the carious lesion. However, the emerging literature suggests that host derived enzymes, and, specially, the matrix metalloproteinases (MMPs) found in the dentine and in the saliva may play an important role in this process, due to their capacity to contribute to degradation of the dentine organic matrix. These facts are important as they enable the study of new therapeutic options for the dental caries lesions prevention and treatment. A literature review was carried out, as the first part of this thesis, to clarify the presence, location and the activity level of the main MMPs found in the human carious tissues,approaching relevant issues, on the local role of the MMPs in regard to this tissue. The selection of the papersdone based on the Pubmed database, in the English language, until November 2014. From 411 eligible papers, 14 were selected for the full study, and 8 were included in this review. From this analysis, respecting the inclusion/exclusion criteria proposed, it can be concluded that the literature has little data on the presence, location and enzymatic activity level in the human carious tissue. Besides this, great differences were found concerning the methodologies used to reach the purposes of their work, and in regard to the classificationof the type of carious dentinal tissue studied. The MMPs more often studied in the carious lesions inhuman dentine were the MMP-2, andMMP-9, followed by MMP-8 and MMP-20. Besides this, it can be suggested that the MMP-2 seems to be, among the studied metalloproteinases, the one in larger quantity in the carious dentinal tissue, mainly in the dentinal tubulesof the affected dentine (internal), proximitywith the pulp tissue. As a second part, the possibility of some fluoride usage as inhibitors of MMPs human salivary has been studied, to interferein the prevention or progressionof carious lesions. The tin fluoride (SnF2), the zinc fluoride (ZnF2) and the titanium tetrafluoride (TiF4), were tested in comparison to the sodium fluoride (NaF), for the inhibition of salivary MMPs. Saliva of healthy donors was used in Zymography, and the solutions were tested in relevant clinical concentrations. The Zymography showed inhibition of the MMP-2 and MMP-9 in decreasing order for the fluorides ZnF2 and TiF4> NaF and SnF2.With the limitations of an in vitro study, it can be suggested that due to the fact, the fluorides tested have shown the capacity of inhibiting the salivary MMP-2 and MMP-9, a new view and perspective arises for the study of these materials in the prevention of dental caries lesion.
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Maguer, Jean-Jacques. "Synthèse, étude structurale et magnétique d'halogénures engageant des éléments 3d ou 4f, dans les systèmes : LiF-ZnF2-InF3, BaF2-BaCl2-MF2 et AF-YbF3(A=H3O+)." Le Mans, 1995. http://www.theses.fr/1995LEMA1001.
Повний текст джерелаАнотація:
Ce trvail de these se developpe en trois etudes concernant la synthese, la determination structurale et la caracterisation de phases solides fluorees. Une etude du systeme ternaire lif-znf#2-inf#3 a permis de preparer, par croissance cristalline en flux de chlorures et par synthese de poudre en phase solide, trois nouvelles phases presentant un empilement quasi hexagonal compact d'ions f#-. Dans ces composes, le taux d'occupations () des sites octaedriques est superieur au taux classiquement observe dans les composes de type amm'f#6 ( = 0. 5): li#4znin#2f#1#2 ( = 0. 583) est un derive de type tri--pbo#2 ; li#5#. #5zn#1#. #2#5in#2f#1#4 ( = 0. 625), et li#6#. #5zn#1#. #7#5in#2f#1#6 ( = 0. 641) sont des polytypes de rutile, respectivement hepta- et tetrarutile. Prealablement, la determination structurale des composes lizninf#6 et limninf#6 a montre l'existence d'un nouvel ordre cationique dans la structure type na#2sif#6. Une nouvelle famille de chlorofluorures ba#2m#2f#7cl et ba#2mm'f#7cl (m, m' = mn#2#+, fe#2#+, co#2#+, ni#2#+, zn#2#+) a egalement ete obtenue par croissance en flux de chlorures. La structure bidimensionnelle de ces composes est constituee d'octaedres mf#5cl qui partagent quatre de leurs sommets pour former des plans gaufres de formulation mx#4. Dans ces plans existe une succession de distances courtes m-f et longues m-cl selon la sequence f-m-cl-m-f. Malgre la presence du chlore, le comportement magnetique de ces composes reste assez similaire a celui des series bamf#4. Cependant, les composes contenant du nickel presentent une forte singularite sous la forme d'un pic de susceptibilite prononce lors de l'apparition probable de l'ordre magnetique 3d. L'etude par diffraction de neutrons a mis en evidence un desordre cationique pour la structure des composes mixtes. Deux types de structures magnetiques presentant chacune un doublement de parametre (vecteur de propagation k = 1/2 o o) ont ete determinees. Ces structures different par l'orientation parallele ou perpendiculaire des moments magnetiques par rapport aux plans d'octaedres, selon l'anisotropie des cations. Elles sont similaires aux structures magnetiques des composes bacof#4 et banif#4. Un processus de chimie basse temperature de precurseurs a permis de synthetiser le compose (h#3o)yb#3f#1#0,h#2o, dont la structure a ete determinee par diffraction des rayons x sur poudre. Sa description structurale est basee sur l'existence d'unites octaedriques d'antiprismes ybf#8, notees uoa##8#, dont le centre est un cube anionique. Sur la base de ces considerations, ce compose est decrit comme un empilement de type carbone diamant d'uoa##8#. Plusieurs composes de la famille aln#3f#1#0 (a=alcalin, ln=terre rare) sont ainsi reconsideres en terme d'empilement compact d'uoa##8# de type cubique f ou hexagonal. Cette description a partir d'unites geometriques de polyedres elementaires (ugp), dont l'empilement constitue un reseau simple (de type cubique f compact, hexagonal compact, carbone diamant,), a ete appliquee a d'autres types structuraux. Cette nouvelle perception des structures complexes a partir d'ugp pourra etre utilisee comme base de reflexion cristallochimique pour la synthese de nouveaux composes
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Chen, Chieh-Ming, and 陳傑明. "Electronic and Atomic Structure Study of ZnFe2-yCryO4 (y=0~2) Electrode for the Electrochemical Reactivity." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/21668291126207762017.
Повний текст джерелаАнотація:
碩士淡江大學
物理學系碩士班
103
In the report, while the Cyclic-Voltage (CV) result of ZnFe2-yCryO4 (y=0~2) showed that the different ratio y have the same oxidation potential but the different reduction potential. Therefore, it is important to explore the connection between electronic and atomic structure by Synchrotron radiation technologies. Extended x-ray absorption fine structure (EXAFS) showed the normal spinel structure, and the EXAFS fitting showed the bond length of Fe-O and Cr-O. X-ray absorption near edge structure (XANES) showed the unoccupied state electronic structure, and X-ray emission spectroscopy (XES) showed the occupied state information. Compared with electronic structure and electrochemical performance, Find out the relationship between the occupied/unoccupied state and charge/discharge potential.
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Datu, Andrea-Kaye. "Regulation of Poly (A)-Binding Protein Expression in Response to Heat Shock and Recovery." Thesis, 2012. http://hdl.handle.net/10214/4056.
Повний текст джерелаАнотація:
Gene expression at the level of mRNA translation is critical for cells to respond to external signals; it allows changes in protein synthesis without triggering transcription of a new set of genes. Control of mRNA translation and stability is important in several cellular processes including cell growth and differentiation. Thus regulation of the cellular machinery involved in mRNA translation is crucial. Poly (A) binding protein (PABP1), eukaryotic elongation factor 1A (eEF1A) and ribosomal protein S6 (RPS6) are important members of the cellular mRNA translation machinery, the mRNAs that encode these proteins belong to the terminal oligo pyrimidine tract (TOP) containing family. Translation of the TOP mRNAs is regulated by growth signals and usually codes for several proteins involved in mRNA translation. Our laboratory has previously reported up regulation of PABP1 mRNA translation during recovery from heat shock. It was also shown that the terminal oligopyrmidine tract (TOP) cis-element of PABP1 mRNA is responsible for the preferential increase of PABP1 mRNA translation; however the mechanism for achieving this is unknown. In the studies reported here, we showed that translation of eEF1A and RPS6 expression was similarly enhanced during recovery from heat shock. Analyses of samples of in vivo cross linked RNA– protein complexes, immunoprecipitated by ZNF9 antibody, for the presence of specific mRNAs showed that the cellular nucleic acid binding protein ZNF9 binds not only to TOP mRNAs but also mRNA that lack the TOP element such as to β-actin mRNA. To elucidate the mechanism of activation of TOP mRNA translation, as a candidate trans acting factor, siRNA was
used to deplete the cellular level of ZNF9 from heat shocked HeLa cells to examine its potential role in stimulation of TOP mRNA translation during recovery from heat shock. Results show that the knock down of ZNF9 disallowed the preferred stimulation of PABP1, eEF1A and RPS6 expression during recovery from heat shock. There was no detectable effect on the constitutive expression of either β-actin or PABP1, eEF1A and RPS6 in exponentially growing HeLa cells. These results suggest that binding of ZNF9 to TOP mRNAs per se does not inhibit translation, but more likely it acts as a general facilitator of mRNA translation. It is possible that modification of the interaction between ZNF9 with other unknown protein factors is responsible for its preferred effect on all three TOP mRNAs studied here. Additionally, results also suggest that a different TOP sequences amongst the observed TOP mRNAs responds similarly to ZNF9.
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Huan-Hsuan and 胡桓軒. "Arginine methylation of ZNF9 and putative differential recognition by anti-Sm autoantibody from SLE patients." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/04454469045144720266.
Повний текст джерелаАнотація:
碩士中山醫學大學
生物醫學科學學系碩士班
95
Anti-Sm is one type of autoantibodies from systemic lupus erythematosus (SLE) patients. Methylation status of specific arginine residues on SmD-1 has been reported to cause differential recognition by anti-Sm. Previous studies in our laboratory used Anti-Sm autosera from three different local SLE patients to analyze AdOx-treated (proteins presumably at hypomethylation state) and untreated (proteins at normal methylation state) HeLa cell extracts by western blots. There were no significant differences between the signals corresponding to SmD1 in samples of different methylation status. However, a few weaker signals were consistently detected from cell extracts treated with AdOx than the ones without. One interesting protein identified by mass spectrometry is ZNF9, which has the GAR domain, a common feature in substrates of protein arginine methyltransferases. We observed that recombinant GST-ZNF9 expressed in Escherichia coli could be methylated by recombinant RMT1 and PRMT1 in vitro. However, no significant differences could be detected between the signals corresponding to GST-ZNF9 of different methylation status after a non-radiation in vitro methylation experiment by western blot with a methylarginine specific antibody 7E6. Therefore, the E. coli expressed recombinant ZNF9 protein could not be used to investigate whether the arginine methylation status affect the recognition of anti-Sm. Nevertheless, FLAG-ZNF9 expressed in HeLa cells could be detected by 7E6 and anti-Sm, while the ones expressed in AdOx-treated HeLa cells has much weaker signals. Furthermore, wild-type FLAG-ZNF9 could be recognized by SYM10 (symmetric dimethylarginine specific antibody) and ASYM24 (asymmetric dimethylarginine specific antibody) but GAR-domain deleted mutant ZNF9 could not. We also observed that FLAG-ZNF9 may interact with two different protein arginine methyltransferase PRMT1 and PRMT5 by an co-immunoprecipitation experiment. In summery, in this study we demonstrate that ZNF9 has both symmetric and asymmetric dimethylarginine modifications and can be differentially recognized by anti-Sm due to the methylation status.
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Δρακόπουλος, Βασίλειος. "Μελέτη της δομής με χρήση της φασματοσκοπίας Raman των υγρών φθοριούχων μιγμάτων : LnF3 - KF (Ln: La, Ce, Nd, Sm, Dy, Yb, Y) και ZnF2 - AF (A:K,Cs)". Thesis, 2000. http://nemertes.lis.upatras.gr/jspui/handle/10889/2297.
Повний текст джерелаЧастини книг з теми "ZNF92"
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Morrison, Clyde Arthur. "ZnF2." In Crystal Fields for Transition-Metal Ions in Laser Host Materials, 48–50. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-95686-7_9.
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"Relationship between the structural modifications and luminescence efficiencies of ZnF2-MO-TeO2 glasses doped with Ho3+ and Er3+ ions." In Current Trends on Lanthanide Glasses and Materials, 136–58. Materials Research Forum LLC, 2017. http://dx.doi.org/10.21741/9781945291159-6.
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Winblad, Stefan, and Anne-Berit Ekström. "Myotonic Dystrophy." In Cognitive and Behavioral Abnormalities of Pediatric Diseases. Oxford University Press, 2010. http://dx.doi.org/10.1093/oso/9780195342680.003.0057.
Повний текст джерелаАнотація:
The myotonic dystrophies, type 1 (DM1) and 2 (DM2) are progressive, autosomal, dominantly inherited disorders, mainly characterized by muscle weakness and atrophy but also by a variable impact on heart, eye, brain, and the endocrine and the gastrointestinal system (Meola 2000). The worldwide prevalence is approximately 1 in 8,000. They are considered to be most common in Western Europe and Japan, but less prevalent in Southeast Asia, and rare or absent in southern and central Africa (Emery 1991). A prevalence of 18 in 340,000 children has been reported (Darin and Tulinius 2000). The cause of myotonic dystrophies is an unstable inherited repeat DNA expansions. Expansions are elements occurring and repeated throughout the human genome, typically polymorphic in the general population. Repeats can become unstable during DNA replication and, depending on specific repeat motif and location, expanded repeats can become pathogenic. In disease states, the number of repeats exceeds the normal range, leading to various pathogenic mechanisms (Ranum and Cooper 2006). DM1 is associated with an expanded (CTG)n repeat (>50 to several thousands) within the noncoding 3′ untranslated region of the myotonic dystrophy protein kinase (DMPK) gene on chromosome 19q13.3. In DM2, another mutation exists, namely an expanded CCTG tetranucleotide repeat (from 75 to 11,000 repeats) in the first intron of the zinc finger protein 9 (ZNF9) gene on chromosome 3q21 (Day and Ranum 2005). This means that two unrelated genes are associated with similar phenotypes although there are differences, including the age of onset and severity of symptoms (Meola 2000). The first signs of a DM2 disease are typically shown in adulthood, and no study has as yet systematically described cognitive or behavioral abnormalities in a childhood DM2 phenotype. Consequently, the following chapter focuses on a description of DM1. In this disorder, the age of onset is variable, meaning that there are congenital cases, as well as children, adults and patients experiencing the first symptoms very late in life. DM1 is traditionally divided into categories, each presenting with specific clinical features and broadly associated with the age of onset and extent of genetic abnormality.
Тези доповідей конференцій з теми "ZNF92"
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de Souza Antero, Giulia, Jaqueline V. Gunha, Aloisi Somer, and Andressa Novatski. "EFEITO DA ADIÇÃO DE ZNF2 EM VIDROS TELURETOS." In XXI Semana da Física. Ponta Grossa, Paraná: Even3, 2018. http://dx.doi.org/10.29327/xxifisica.128783.
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Banshchikov, A. G., N. F. Kartenko, A. K. Kaveev, M. M. Moisseeva, and Nikolai S. Sokolov. "Growth and structural characterization of ZnF2 epitaxial layers on Si." In SPIE Proceedings, edited by Zhores I. Alferov and Leo Esaki. SPIE, 2002. http://dx.doi.org/10.1117/12.510497.
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Naresh, V., and S. Buddhudu. "VIS-NIR emission analysis of Tm3+ doped LiF-ZnF2-AlF3-B2O3-SiO2 glasses." In NANOFORUM 2014. AIP Publishing LLC, 2015. http://dx.doi.org/10.1063/1.4917890.
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Kochanowicz, Marcin, and Dominik Dorosz. "Thermal and optical properties of SiO2-Al2O3-PbF2-ZnF2-ZnO-LaF3-Nd2O3 glasses." In Optical Fibers and Their Applications 2008. SPIE, 2008. http://dx.doi.org/10.1117/12.804518.
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