Готові списки джерел за темами / Yeast Cell Surface

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Автор: Grafiati
Опубліковано: 6 вересня 2023

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Статті в журналах з теми "Yeast Cell Surface"

1

Bae, Jungu, Kouichi Kuroda, and Mitsuyoshi Ueda. "Proximity Effect among Cellulose-Degrading Enzymes Displayed on the Saccharomyces cerevisiae Cell Surface." Applied and Environmental Microbiology 81, no. 1 (October 10, 2014): 59–66. http://dx.doi.org/10.1128/aem.02864-14.

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ABSTRACTProximity effect is a form of synergistic effect exhibited when cellulases work within a short distance from each other, and this effect can be a key factor in enhancing saccharification efficiency. In this study, we evaluated the proximity effect between 3 cellulose-degrading enzymes displayed on theSaccharomyces cerevisiaecell surface, that is, endoglucanase, cellobiohydrolase, and β-glucosidase. We constructed 2 kinds of arming yeasts through genome integration: ALL-yeast, which simultaneously displayed the 3 cellulases (thus, the different cellulases were near each other), and MIX-yeast, a mixture of 3 kinds of single-cellulase-displaying yeasts (the cellulases were far apart). The cellulases were tagged with a fluorescence protein or polypeptide to visualize and quantify their display. To evaluate the proximity effect, we compared the activities of ALL-yeast and MIX-yeast with respect to degrading phosphoric acid-swollen cellulose after adjusting for the cellulase amounts. ALL-yeast exhibited 1.25-fold or 2.22-fold higher activity than MIX-yeast did at a yeast concentration equal to the yeast cell number in 1 ml of yeast suspension with an optical density (OD) at 600 nm of 10 (OD10) or OD0.1. At OD0.1, the distance between the 3 cellulases was greater than that at OD10 in MIX-yeast, but the distance remained the same in ALL-yeast; thus, the difference between the cellulose-degrading activities of ALL-yeast and MIX-yeast increased (to 2.22-fold) at OD0.1, which strongly supports the proximity effect between the displayed cellulases. A proximity effect was also observed for crystalline cellulose (Avicel). We expect the proximity effect to further increase when enzyme display efficiency is enhanced, which would further increase cellulose-degrading activity. This arming yeast technology can also be applied to examine proximity effects in other diverse fields.
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2

Nayyar, Ashima, Graeme Walker, Elisabetta Canetta, Forbes Wardrop, and Ashok K. Adya. "Influence of Cell Surface and Nanomechanical Properties on the Flocculation Ability of Industrial Saccharomyces cerevisiae Strains." Journal of Food Research 6, no. 5 (August 2, 2017): 1. http://dx.doi.org/10.5539/jfr.v6n5p1.

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In the past few years, atomic force microscopy (AFM) has provided novel information on the ultrastructural and nanomechanical properties of yeast cell walls that play a major role in determining the flocculation characteristics of the yeasts. In this study, we used AFM to visualize at the nanoscale the cell surface topography and to determine cell wall nanomechanical properties (e.g. elasticity and adhesion) of different strains of S. cerevisiae employed for brewing, winemaking and fuel alcohol production. Cell surface topography was found to correlate with the flocculation behaviour of these strains during their late stationary phase, with the cell surface of flocculent cells being rougher than that of weakly flocculent cells. The elastic modulus of the yeast cell walls showed that weakly flocculent strains had a more rigid cell wall than highly flocculent strains. This difference in elasticity seemed to have an effect on the adhesive properties of the yeast cell walls, with weakly flocculent yeasts displaying lower adhesion energy than the highly flocculent strains. These findings seem to indicate that yeast cell surface nanomechanical properties play an important role in governing flocculation.
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3

Shibasaki, Seiji, and Mitsuyoshi Ueda. "Progress of Molecular Display Technology Using Saccharomyces cerevisiae to Achieve Sustainable Development Goals." Microorganisms 11, no. 1 (January 3, 2023): 125. http://dx.doi.org/10.3390/microorganisms11010125.

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In the long history of microorganism use, yeasts have been developed as hosts for producing biologically active compounds or for conventional fermentation. Since the introduction of genetic engineering, recombinant proteins have been designed and produced using yeast or bacterial cells. Yeasts have the unique property of expressing genes derived from both prokaryotes and eukaryotes. Saccharomyces cerevisiae is one of the well-studied yeasts in genetic engineering. Recently, molecular display technology, which involves a protein-producing system on the yeast cell surface, has been established. Using this technology, designed proteins can be displayed on the cell surface, and novel abilities are endowed to the host yeast strain. This review summarizes various molecular yeast display technologies and their principles and applications. Moreover, S. cerevisiae laboratory strains generated using molecular display technology for sustainable development are described. Each application of a molecular displayed yeast cell is also associated with the corresponding Sustainable Development Goals of the United Nations.
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4

INOKUMA, Kentaro, and Tomohisa HASUNUMA. "Evolution of Yeast Cell Surface Engineering." Oleoscience 22, no. 3 (2022): 99–105. http://dx.doi.org/10.5650/oleoscience.22.99.

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5

Bagnat, M., and K. Simons. "Cell surface polarization during yeast mating." Proceedings of the National Academy of Sciences 99, no. 22 (October 8, 2002): 14183–88. http://dx.doi.org/10.1073/pnas.172517799.

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6

Shimoi, Hitoshi, Kazutoshi Sakamoto, Masaki Okuda, Ratchanee Atthi, Kazuhiro Iwashita, and Kiyoshi Ito. "The AWA1 Gene Is Required for the Foam-Forming Phenotype and Cell Surface Hydrophobicity of Sake Yeast." Applied and Environmental Microbiology 68, no. 4 (April 2002): 2018–25. http://dx.doi.org/10.1128/aem.68.4.2018-2025.2002.

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ABSTRACT Sake, a traditional alcoholic beverage in Japan, is brewed with sake yeasts, which are classified as Saccharomyces cerevisiae. Almost all sake yeasts form a thick foam layer on sake mash during the fermentation process because of their cell surface hydrophobicity, which increases the cells' affinity for bubbles. To reduce the amount of foam, nonfoaming mutants were bred from foaming sake yeasts. Nonfoaming mutants have hydrophilic cell surfaces and no affinity for bubbles. We have cloned a gene from a foam-forming sake yeast that confers foaming ability to a nonfoaming mutant. This gene was named AWA1 and structures of the gene and its product were analyzed. The N- and C-terminal regions of Awa1p have the characteristic sequences of a glycosylphosphatidylinositol anchor protein. The entire protein is rich in serine and threonine residues and has a lot of repetitive sequences. These results suggest that Awa1p is localized in the cell wall. This was confirmed by immunofluorescence microscopy and Western blotting analysis using hemagglutinin-tagged Awa1p. Moreover, an awa1 disruptant of sake yeast was hydrophilic and showed a nonfoaming phenotype in sake mash. We conclude that Awa1p is a cell wall protein and is required for the foam-forming phenotype and the cell surface hydrophobicity of sake yeast.
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7

Thiebault, F., and J. Coulon. "Influence of carbon source and surface hydrophobicity on the aggregation of the yeastKluyveromyces bulgaricus." Canadian Journal of Microbiology 51, no. 1 (January 1, 2005): 91–94. http://dx.doi.org/10.1139/w04-106.

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Aggregation of the yeast Kluyveromyces bulgaricus is mediated by the galactose-specific lectin KbCWL1. This lectin contains hydrophobic amino acids and its activity is calcium dependent. A specific fluorescent probe, 1-anilinonaphthalene-8-sulfonic acid in the free acid form (ANS; Sigma Chemical Co., St. Louis, Missouri), was used to study the hydrophobic areas on the cellular surface of K. bulgaricus. Changes in surface hydrophobicity during the growth and aggregation of yeast cells were studied. Surface hydrophobicity increased during growth and depended on the amount of yeast cells in the culture medium. During growth, the size of the hydrophobic areas on the cell surface was measured using ANS and was found to increase with the percentage of flocculating yeasts. Our results strongly suggest that the hydrophobic areas of the cell walls of yeast cells are involved in the aggregation of K. bulgaricus.Key words: aggregation, carbon source, fluorescence probe, hydrophobicity, yeast.
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8

Shipingana, N. N., N. Raghu, S. Veerana Gowda, T. S. Gopenath, M. S. Ranjith, A. Gnanasekaran, M. Karthikeyan, et al. "Cell signaling in yeast: A mini review." Journal of Biomedical Sciences 5, no. 2 (April 17, 2019): 18–22. http://dx.doi.org/10.3126/jbs.v5i2.23634.

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Background: Understanding cellular mechanism of communication is the main goal of systems biology. Unicellular yeasts are effective model to understand the molecular interactions that generate cell polarity induced by external inputs. The mechanisms of many extracellular stimuli are induced by complexes of cell surface receptors, G proteins. The mechanisms of many extracellular stimuli are induced by complexes of cell surface receptors, G proteins and mitogen activated protein (MAP) kinase complexes. Many components, their interrelationships, and their regulators of these mechanisms were initially identified in yeast. A complex web of sensing mechanisms and cooperation among signaling networks such as a cyclic adenosine monophosphate dependent protein kinase, mitogen-activated protein kinase cascade and 5-adenosine monophosphate activated protein kinase induce various changes in physiology, cell polarity, cell cycle progression and gene expression to achieve differentiation. Ras-cAMP pathway explained in yeast model with signalling function of the oncogenic mammalian Ras protein. So studies on yeast cells may enlighten some underlying mechanism which will be beneficial to understand the mechanisms of disease.
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9

Shibasaki, Seiji, Yuki Nakatani, Kazuaki Taketani, Miki Karasaki, Kiyoshi Matsui, Mitsuyoshi Ueda, and Tsuyoshi Iwasaki. "Construction of HGF-Displaying Yeast by Cell Surface Engineering." Microorganisms 10, no. 7 (July 7, 2022): 1373. http://dx.doi.org/10.3390/microorganisms10071373.

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Hepatocyte growth factor (HGF) has been investigated as a regulator for immune reactions caused by transplantation and autoimmune diseases and other biological functions. Previous studies demonstrated that cDNA-encoding HGF administration could inhibit acute graft-versus-host disease (GVHD) after treatment via hematopoietic stem cell transplantation. This study aimed to show the preparation of HGF protein on yeast cell surfaces to develop a tool for the oral administration of HGF to a GVHD mouse model. In this study, full-length HGF and the heavy chain of HGF were genetically fused with α-agglutinin and were successfully displayed on the yeast cell surface. This study suggested that yeast cell surface display engineering could provide a novel administration route for HGF.
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10

Coleman, David A., Soon-Hwan Oh, Xiaomin Zhao, and Lois L. Hoyer. "Heterogeneous distribution of Candida albicans cell-surface antigens demonstrated with an Als1-specific monoclonal antibody." Microbiology 156, no. 12 (December 1, 2010): 3645–59. http://dx.doi.org/10.1099/mic.0.043851-0.

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Despite an abundance of data describing expression of genes in the Candida albicans ALS (agglutinin-like sequence) gene family, little is known about the production of Als proteins on individual cells, their spatial localization or stability. Als proteins are most commonly discussed with respect to function in adhesion of C. albicans to host and abiotic surfaces. Development of a mAb specific for Als1, one of the eight large glycoproteins encoded by the ALS family, provided the opportunity to detect Als1 during growth of yeast and hyphae, both in vitro and in vivo, and to demonstrate the utility of the mAb in blocking C. albicans adhesion to host cells. Although most C. albicans yeast cells in a saturated culture are Als1-negative by indirect immunofluorescence, Als1 is detected on the surface of nearly all cells shortly after transfer into fresh growth medium. Als1 covers the yeast cell surface, with the exception of bud scars. Daughters of the inoculum cells, and sometimes granddaughters, also have detectable Als1, but Als1 is not detectable on cells from subsequent generations. On germ tubes and hyphae, most Als1 is localized proximal to the mother yeast. Once deposited on yeasts or hyphae, Als1 persists long after the culture has reached saturation. Growth stage-dependent production of Als1, coupled with its persistence on the cell surface, results in a heterogeneous population of cells within a C. albicans culture. Anti-Als1 immunolabelling patterns vary depending on the source of the C. albicans cells, with obvious differences between cells recovered from culture and those from a murine model of disseminated candidiasis. Results from this work highlight the temporal parallels for ALS1 expression and Als1 production in yeasts and germ tubes, the specialized spatial localization and persistence of Als1 on the C. albicans cell surface, and the differences in Als1 localization that occur in vitro and in vivo.
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Дисертації з теми "Yeast Cell Surface"

1

Nayyar, Ashima. "Yeast flocculation : understanding cell surface structure-function relationships in industrial yeast strains." Thesis, Abertay University, 2015. https://rke.abertay.ac.uk/en/studentTheses/cec13693-e667-4426-ba6c-6873e5c2b642.

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Adhesion properties of microorganisms are crucial for many essential biological processes such as sexual reproduction, tissue or substrate invasion, biofilm formation and cell-cell aggregation. One of such controlled forms of cellular adhesion in yeast that occurs preferentially in the liquid environments is a process of asexual aggregation of cells which is also referred to as flocculation. The timing during growth and the causes of onset of yeast flocculation are of commercial interest to the brewing industry, as flocculation can determine the degree of attenuation of the wort. Early or premature flocculation is one common causes of ‘hung’ or ‘stuck’ fermentations giving rise to sweeter beer whereas a lack or delay in flocculation can cause filtration difficulties and some problems in obtaining a bright sparkling beer; in addition, the presence of excess yeast in beer during ageing can cause off flavours due to yeast autolysis. Despite this commercial interest, limited information is available about the onset of flocculation and the various factors that may be responsible in the process. In particular, what are the signals that trigger flocculation? Adhesion properties applicable in improving yeast biotechnology are dependent directly or indirectly on characteristics of cellular surfaces, usually the outer layer of the cell wall. Change in the structure and or composition of the cell wall leads to changes in the microbial adhesion properties. Exploring more into the cell wall and studying the nanoscale structure of the yeast cell wall would thus be beneficial to augment our understanding of flocculation.
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2

Proszynski, Tomasz. "Protein sorting and cell surface polarity in yeast." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2005. http://nbn-resolving.de/urn:nbn:de:swb:14-1131974045019-73555.

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The studies presented here were focused on the understanding of the principles for protein sorting from the Golgi to the cell surface. As a marker protein we used Fus1p, a type I plasma membrane protein that is O-glycosylated on the extracellular domain and plays a role in cell fusion during yeast mating. Additionally, we analyzed mechanisms responsible for asymmetric distribution of Fus1p in mating cells. We demonstrated that the glycans attached to the protein act as a sorting determinant for protein transport to the cell surface. In cells lacking PMT4, encoding a mannosyltransferase involved in the initial step of O-glycosylation, Fus1p was not glycosylated and accumulated in late Golgi structures. A similar defect in exocytosis was observed when a Fus1p mutant lacking the O-glycosylated domain was expressed in wild-type cells, however, the cell surface delivery could be rescued if the 33 amino acid portion of the Fus1p ectodomain, containing 15 potentially glycosylated sites was added to the protein. It was previously well documented in epithelial cells that different types of protein glycosylation and association with lipid rafts play a role of determinants for protein delivery to the apical plasma membrane. However, otherwise the machinery responsible for cargo sorting to the apical membrane is poorly understood. Our finding that also in yeast, protein glycosylation can function as a sorting determinant provides a new possibility to investigate underlying mechanisms...
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Foster, Alexander J. "Cell surface analysis of the basidiomycete yeast cryptococcus neoformans." Thesis, Aston University, 2004. http://publications.aston.ac.uk/11011/.

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Cell surface properties of the basidiomycete yeast Cryptococcus neoformans were investigated with a combination of novel and well proven approaches. Non-specific cell adhesion forces, as well as exposed carbohydrate and protein moieties potentially associated with specific cellular interaction, were analysed. Experimentation and analysis employed cryptococcal cells of different strains, capsular status and culture age. Investigation of cellular charge by particulate microelectrophoresis revealed encapsulated yeast forms of C. neoformans manifest a distinctive negative charge regardless of the age of cells involved; in turn, the neutral charge of acapsulate yeasts confirmed that the polysaccharide capsule, and not the cell wall, was responsible for this occurrence. Hydrophobicity was measured by MATH and HICH techniques, as well as by the attachment of polystyrene microspheres. All three techniques, where applicable, found C. neoformans yeast to be consistently hydrophilic; this state varied little regardless of strain and culture age. Cell surface carbohydrates and protein were investigated with novel fluorescent tagging protocols, flow cytometry and confocal microscopy. Cell surface carbohydrate was identified by controlled oxidation in association with biotin hydrazide and fluorescein-streptavidin tagging. Marked amounts of carbohydrate were measured and observed on the cell wall surface of cryptococcal yeasts. Furthermore, tagging of carbohydrates with selective fluorescent lectins supported the identification, measurement and observation of substantial amounts of mannose, glucose and N-acetyl-glucosamine. Cryptococcal cell surface protein was identified using sulfo-NHS-biotin with fluorescein-streptavidin, and then readily quantified by flow cytometry.
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4

George, Ellen. "The influence of brewing yeast physiology on cell surface properties." Thesis, Oxford Brookes University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.318535.

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5

Murai, Toshiyuki. "Studies on genetic display of hydrolytic enzymes on yeast cell surface." Kyoto University, 1998. http://hdl.handle.net/2433/182354.

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Shiraga, Seizaburo. "Studies on modification of functions of hydrolytic enzymes by yeast cell surface engineering." 京都大学 (Kyoto University), 2005. http://hdl.handle.net/2433/144560.

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Kyoto University (京都大学)
0048
新制・課程博士
博士(工学)
甲第11889号
工博第2582号
新制||工||1362(附属図書館)
23669
UT51-2005-N723
京都大学大学院工学研究科合成・生物化学専攻
(主査)教授 森 泰生, 教授 今中 忠行, 教授 青山 安宏
学位規則第4条第1項該当
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Zou, Wen. "STUDIES ON THE NEW FUNCTIONAL YEAST STRAINS CONSTRUCTED AND SCREENED BY CELL SURFACE ENGINEERING." 京都大学 (Kyoto University), 2001. http://hdl.handle.net/2433/150264.

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8

Hennig, Stefan. "Utilization of yeast pheromones and hydrophobin-based surface engineering for novel whole-cell sensor applications." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-223271.

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Whole-cell sensors represent an emerging branch in biosensor development since they obviate the need for enzyme/antibody purification and provide the unique opportunity to assess global parameters such as genotoxicity and bioavailability. Yeast species such as Saccharomyces cerevisiae are ideal hosts for whole-cell sensor applications. However, current approaches almost exclusively rely on analyte-induced expression of fluorescent proteins or luciferases that imply issues with light scattering and/or require the supply of additional substrates. In this study, the yeast α-factor mating pheromone, a peptide pheromone involved in cell-cell communication in Saccharomyces cerevisiae, was utilized to create the whole-cell sensor read-out signal, in particular by employing engineered sensor cells that couple the response to a user-defined environmental signal to α-factor secretion. Two novel immunoassays - relying on hydrophobin-based surface engineering - were developed to quantify the α-factor. Hydrophobins are amphiphilic fungal proteins that self-assemble into robust monolayers at hydrophobic surfaces. Two recombinant hydrophobins, either lacking (EAS) or exposing the α-factor pheromone (EAS-α) upon self-assembly, were used to functionalize polystyrene supports. In a first approach (competitive immunoassay), pheromone-specific antibodies initially bound to the functionalized surface (due to the α-factor exposed by the hydrophobin layer) were competitively detached by soluble α-factor. In a second approach, the antibodies were first premixed with pheromone-containing samples and subsequently applied to functionalized surfaces, allowing for the attachment of antibodies that still carried available binding sites (inverse immunoassay). Both immunoassays enabled quantitative assessment of the yeast pheromone in a unique but partially overlapping dynamic range and allowed for facile tuning of the assay sensitivity by adjustment of the EAS-α content of the hydrophobin layer. With a limit of detection of 0.1 nM α-factor, the inverse immunoassay proved to be the most sensitive pheromone quantification assay currently available. Due to the high stability of hydrophobin monolayers, functionalized surfaces could be reused for multiple consecutive measurements. Favorably, both immunoassays proved to be largely robust against the changes in the sample matrix composition, allowing for pheromone quantification in complex sample matrices such as yeast culture supernatants. Hence, these immunoassays could also be applied to study the pheromone secretion of wild-type and engineered Saccharomyces cerevisiae strains. Additionally, a proof-of-concept whole-cell sensor for thiamine was developed by combining the hydrophobin-based immunoassays with engineered sensor cells of Schizosaccharomyces pombe modulating the secretion of the α-factor pheromone in response to thiamine. Since this read-out strategy encompasses intrinsic signal amplification and enables flexible choice of the transducer element, it could contribute to the development of miniaturized, portable whole-cell sensors for on-site application.
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Chetty, Bronwyn Jean. "Improvement of cell-surface adhered cellulase activities in recombinant strains of Saccharomyces cerevisiae engineered for consolidated bioprocessing." University of Western Cape, 2021. http://hdl.handle.net/11394/8357.

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>Magister Scientiae - MSc
Consolidated bioprocessing (CBP), in which a single organism in a single reactor is responsible for the conversion of pretreated lignocellulosic biomass to bioethanol, remains an attractive option for production of commodity products if an organism fit for this process can be engineered. The yeast Saccharomyces cerevisiae requires engineered cellulolytic activity to enable its use in CBP production of second generation bioethanol. Current recombinant yeast strains engineered for this purpose must overcome the drawback of generally low secretion titres. A promising strategy for directly converting lignocellulose to ethanol is by displaying heterologous cellulolytic enzymes on the cell surface by means of the glycosylphosphatidylinositol (GPI) or similar anchoring systems. Recently, a strain producing cell-adhered enzymes in a ratio-optimized manner was created that showed significant crystalline cellulose hydrolysis.
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Hennig, Stefan, Gerhard Rödel, and Kai Ostermann. "Hydrophobin-Based Surface Engineering for Sensitive and Robust Quantification of Yeast Pheromones." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-214807.

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Detection and quantification of small peptides, such as yeast pheromones, are often challenging. We developed a highly sensitive and robust affinity-assay for the quantification of the α-factor pheromone of Saccharomyces cerevisiae based on recombinant hydrophobins. These small, amphipathic proteins self-assemble into highly stable monolayers at hydrophilic-hydrophobic interfaces. Upon functionalization of solid supports with a combination of hydrophobins either lacking or exposing the α-factor, pheromone-specific antibodies were bound to the surface. Increasing concentrations of the pheromone competitively detached the antibodies, thus allowing for quantification of the pheromone. By adjusting the percentage of pheromone-exposing hydrophobins, the sensitivity of the assay could be precisely predefined. The assay proved to be highly robust against changes in sample matrix composition. Due to the high stability of hydrophobin layers, the functionalized surfaces could be repeatedly used without affecting the sensitivity. Furthermore, by using an inverse setup, the sensitivity was increased by three orders of magnitude, yielding a novel kind of biosensor for the yeast pheromone with the lowest limit of detection reported so far. This assay was applied to study the pheromone secretion of diverse yeast strains including a whole-cell biosensor strain of Schizosaccharomyces pombe modulating α-factor secretion in response to an environmental signal.
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Книги з теми "Yeast Cell Surface"

1

Ueda, Mitsuyoshi, ed. Yeast Cell Surface Engineering. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-5868-5.

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2

George, Ellen. The influence of brewing yeast physiology on cell surface properties. Oxford: Oxford Brookes University, 1996.

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3

Rhymes, Maureen Ruth. The effect of starvation on brewing yeast cell surface physical characteristics. Oxford: Oxford Brookes University, 1998.

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4

Ueda, Mitsuyoshi. Yeast Cell Surface Engineering: Biological Mechanisms and Practical Applications. Springer, 2019.

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5

Read, Nick D. Fungal cell structure and organization. Edited by Christopher C. Kibbler, Richard Barton, Neil A. R. Gow, Susan Howell, Donna M. MacCallum, and Rohini J. Manuel. Oxford University Press, 2017. http://dx.doi.org/10.1093/med/9780198755388.003.0004.

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Human pathogenic fungi produce three basic ‘cell’ types: hyphae, yeast cells, and spores. The organization and subcellular structure of these different cell types and their modes of growth and formation are reviewed. Growth and form is the consequence of how new cell surface is formed. This is generated by the delivery of vesicles to the surface which provides new membrane and the enzymes for cell wall synthesis. To generate these various cell types, the pathway of vesicle secretion to the surface has to be carefully regulated. These vesicles have to be transported through the cell by the cytoskeleton, and in filamentous cells these vesicles accumulate at a supply centre called the Spitzenkörper before docking and fusion with the hyphal apex. Ultimately, membrane is also endocytosed and recycled behind actively expanding regions of the fungal surface. These various processes are described and particular emphasis is given to the structural and organizational features of fungal cells that play roles in their pathogenesis and virulence.
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Частини книг з теми "Yeast Cell Surface"

1

Ueda, Mitsuyoshi. "Principle of Cell Surface Engineering of Yeast." In Yeast Cell Surface Engineering, 3–14. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-5868-5_1.

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2

Aoki, Wataru. "Engineering Antibodies and Alternative Binders for Therapeutic Uses." In Yeast Cell Surface Engineering, 123–47. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-5868-5_10.

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Shibasaki, Seiji. "Oral Vaccine Development Using Cell Surface Display Technology." In Yeast Cell Surface Engineering, 149–58. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-5868-5_11.

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Ueda, Mitsuyoshi. "Combinatorial Engineering." In Yeast Cell Surface Engineering, 161–73. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-5868-5_12.

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Miura, Natsuko. "Enzyme Evolution." In Yeast Cell Surface Engineering, 175–85. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-5868-5_13.

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Kuroda, Kouichi. "Energy Production: Biomass – Starch, Cellulose, and Hemicellulose." In Yeast Cell Surface Engineering, 17–28. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-5868-5_2.

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Takagi, Toshiyuki. "Energy Production: Biomass – Marine." In Yeast Cell Surface Engineering, 29–41. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-5868-5_3.

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Ogino, Chiaki, and Jerome Amoah. "Energy Production: Biodiesel." In Yeast Cell Surface Engineering, 43–61. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-5868-5_4.

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Kuroda, Kouichi. "Cleanup of Pollution: Heavy Metal Ions and Environmental Hormones." In Yeast Cell Surface Engineering, 63–72. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-5868-5_5.

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Kuroda, Kouichi. "Recovery of Rare Metal Ions." In Yeast Cell Surface Engineering, 73–83. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-5868-5_6.

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Тези доповідей конференцій з теми "Yeast Cell Surface"

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Fung, Tracy H., Gregory I. Ball, Sarah C. McQuaide, Shih-Hui Chao, Alejandro Coleman-Lerner, Mark R. Holl, and Deirdre R. Meldrum. "Microprinting of On-Chip Cultures: Patterning of Yeast Cell Microarrays Using Concanavalin-A Adhesion." In ASME 2004 International Mechanical Engineering Congress and Exposition. ASMEDC, 2004. http://dx.doi.org/10.1115/imece2004-60866.

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Microprinting has been demonstrated effective in the patterning of surface regions for trapping cells used within microfluidic devices. In this study a polydimethylsiloxane (PDMS) silicone elastomer stamp was microfabricated and used to microstamp concanavalin-A (con-A; protein that binds to yeast) on a glass surface. Yeast cells, Saccharomyces cerevisiae, were brought into contact with patterned con-A producing an array of yeast microscale culture in an ordered array identical to the printed pattern.
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Marynchenko, L., O. Nizhelska, A. Kurylyuk, V. Makara, and S. Naumenko. "Observed effects of electromagnetic fields action on yeast and bacteria cells attached to surfaces." In 2020 IEEE 40th International Conference on Electronics and Nanotechnology (ELNANO). IEEE, 2020. http://dx.doi.org/10.1109/elnano50318.2020.9088883.

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Melhado, Eliana Meire, Letícia Buzzo do Amaral, Leonardo Estrela Thomé, Cibele Alexandra Ferro, Marcelo Freitas Martins, Natalia Prando, and Alexandre Haddad de Souza. "Neurocriptococcosis in an immunocompetent patient: a case report." In XIV Congresso Paulista de Neurologia. Zeppelini Editorial e Comunicação, 2023. http://dx.doi.org/10.5327/1516-3180.141s1.377.

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Introduction: Cryptococcosis is an infection acquired through the lungs, the form of central nervous system involvement is neurocriptococcosis. The diagnosis is the investigation of yeasts in the cerebrospinal fluid using china ink. Treatment is amphotericin B and fluconazole. The aim is to report a case of neurocriptococcosis in an immunocompetent patient. This is a descriptive study, through the analysis of the medical record. Case report: Female, 23 years old, healthy, with severe headache for 15 days, with progressive worsening, neurological examination without changes, performed skull computed tomography (CT) scan, no changes, cerebrospinal fluid (CSF) examination showed nucleated cells 204, presence of yeast, Venereal Disease Research Laboratory (VDRL): non-reactive and culture of Cryptococcus gattii, positive China ink. Magnetic resonance imaging of the brain showed hypersignal on T2/FLAIR (T2-weighted-Fluid-Attenuated Inversion Recovery) affecting cortical sulci, encephalic fissures, cerebellar foliae and pial surface of the brainstem and diffuse leptomeningeal impregnation, volumetric reduction of the supratentorial ventricular system, suggestive of cerebrospinal fluid hypotension, findings of leptomeningitis, related to cryptococcosis. Treatment with amphotericin B and fluconazole was initiated. Patient with onset of focal neurological deficits, amaurosis and dysarthria. She presented with refractory headache, requiring serial liquoric punctures for relief of intracranial hypertension. She evolved with instability and underwent a right ventriculoperitoneal shunt. Postoperatively, he presented left hemiparesis. Skull CT showed hematoma and emphysema of adjacent extracranial soft tissues; right frontoparietal brain lesion and edema of adjacent parenchyma. She evolved with clinical instability and died after 61 days of hospitalization. Conclusion: A pattern of CSF hypotension was shown due to compression of the frontal horns of the lateral ventricles by the granulomas. There was a need for ventriculoperitoneal shunt, but patient had fatal complications.
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Stanojević-Nikolić, Slobodanka, Milan P. Nikolić, Marina Šćiban, Vladimir V. Srdić, and Vladimir B. Pavlović. "KINETIC AND EQUILIBRIUM STUDIES OF BIOSORPTION OF Cd(II) IONS USING SILICA-ALGINATE-YEAST COMPOSITE." In 1st International Symposium on Biotechnology. University of Kragujevac, Faculty of Agronomy, 2023. http://dx.doi.org/10.46793/sbt28.323sn.

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Viable Saccharomyces cerevisiae cells were immobilized by silicaalginate matrix and obtained spherical composite particles and used for biosorption of Cd(II) ions. The obtained composite displayed high cadmium removal efficiency of 99.2, 95.7, 88.3 and 78% in the successive four adsorption steps. The adsorption capacity after four step of Cd(II) removal was ~14,2 mg of Cd(II) per g of biosorbent. Pseudo-second-order kinetic agree well with the experimental values suggesting both adsorption and ionic exchange are simultaneously performed on the surface of used biosorbent.
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JAWAD, Israa, Adian Abd Alrazak DAKL, and Hussein Jabar JASIM. "CHARACTERIZATION, MECHANISM OF ACTION, SOURCES TYPES AND USES OF THE ANTIMICROBIAL PEPTIDES IN DOMESTIC ANIMALS, REVIEW." In VII. INTERNATIONAL SCIENTIFIC CONGRESSOF PURE,APPLIEDANDTECHNOLOGICAL SCIENCES. Rimar Academy, 2023. http://dx.doi.org/10.47832/minarcongress7-13.

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This review aimed to identify the general characteristics of , mechanism of action, types and uses of antimicrobial peptides in animals, antimicrobial peptides were lass of small peptides that widely exist naturally, they varied greatly in structure, composition are found in the animal's species, and were standard structural features, twenty to sixty residue long, cationic and amphipathic peptides, have a positive charge that interacted with negatively charged molecules on the bacterial cell surfaces, a have an expansive field of inhibitory effects and were made as the first line of protection by both multicellular organisms. An essential component of the innate immune method of various organisms can have broad movement to instantly destroy bacteria, parasites, yeasts, fungi, viruses, and even cancer cells, Several antimicrobial peptides were expressed in the gastrointestinal mucosa of the animals where they can modulate innate immune responses and the intestinal microbial, act some protective microbial species and modulate an immune response. Its interactions with innate immunity and the intestinal microbial reveal attractive drug targets, act as a new therapeutic approach against gastrointestinal infections, damage, and inflammations, and modulate obesity and metabolic diseases. In addition, its acts as a biomarker of gastrointestinal diseases. They have been useful parts of the host's defense systems for a long time. Because microbes become resistant to antimicrobial peptides more slowly than to traditional antibiotics, they could be used as alternative treatments in the future. Several thousand antimicrobial peptides have been isolated from microorganisms, plants, insects, crustaceans, creatures, and even humans. Conclusion: Antimicrobial peptides are small proteins found in plant and animal species. They are the first defense against infections caused by microorganisms. and work against a wide range of bacteria, fungi, and viruses, both gram-positive and gram-negative. They are related together to innate immunity and adaptive immunity.
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Звіти організацій з теми "Yeast Cell Surface"

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Droby, Samir, Michael Wisniewski, Ron Porat, and Dumitru Macarisin. Role of Reactive Oxygen Species (ROS) in Tritrophic Interactions in Postharvest Biocontrol Systems. United States Department of Agriculture, December 2012. http://dx.doi.org/10.32747/2012.7594390.bard.

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To elucidate the role of ROS in the tri-trophic interactions in postharvest biocontrol systems a detailed molecular and biochemical investigation was undertaken. The application of the yeast biocontrol agent Metschnikowia fructicola, microarray analysis was performed on grapefruit surface wounds using an Affymetrix Citrus GeneChip. the data indicated that 1007 putative unigenes showed significant expression changes following wounding and yeast application relative to wounded controls. The expression of the genes encoding Respiratory burst oxidase (Rbo), mitogen-activated protein kinase (MAPK) and mitogen-activated protein kinase kinase (MAPKK), G-proteins, chitinase (CHI), phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS) and 4-coumarate-CoA ligase (4CL). In contrast, three genes, peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT), were down-regulated in grapefruit peel tissue treated with yeast cells. The yeast antagonists, Metschnikowia fructicola (strain 277) and Candida oleophila (strain 182) generate relatively high levels of super oxide anion (O2−) following its interaction with wounded fruit surface. Using laser scanning confocal microscopy we observed that the application of M. fructicola and C. oleophila into citrus and apple fruit wounds correlated with an increase in H2O2 accumulation in host tissue. The present data, together with our earlier discovery of the importance of H₂O₂ production in the defense response of citrus flavedo to postharvest pathogens, indicate that the yeast-induced oxidative response in fruit exocarp may be associated with the ability of specific yeast species to serve as biocontrol agents for the management of postharvest diseases. Effect of ROS on yeast cells was also studied. Pretreatment of the yeast, Candida oleophila, with 5 mM H₂O₂ for 30 min (sublethal) increased yeast tolerance to subsequent lethal levels of oxidative stress (50 mM H₂O₂), high temperature (40 °C), and low pH (pH 4). Suppression subtractive hybridization analysis was used to identify genes expressed in yeast in response to sublethal oxidative stress. Transcript levels were confirmed using semi quantitative reverse transcription-PCR. Seven antioxidant genes were up regulated. Pretreatment of the yeast antagonist Candida oleophila with glycine betaine (GB) increases oxidative stress tolerance in the microenvironment of apple wounds. ROS production is greater when yeast antagonists used as biocontrol agents are applied in the wounds. Compared to untreated control yeast cells, GB-treated cells recovered from the oxidative stress environment of apple wounds exhibited less accumulation of ROS and lower levels of oxidative damage to cellular proteins and lipids. Additionally, GB-treated yeast exhibited greater biocontrol activity against Penicillium expansum and Botrytis cinerea, and faster growth in wounds of apple fruits compared to untreated yeast. The expression of major antioxidant genes, including peroxisomal catalase, peroxiredoxin TSA1, and glutathione peroxidase was elevated in the yeast by GB treatment. A mild heat shock (HS) pretreatment (30 min at 40 1C) improved the tolerance of M. fructicola to subsequent high temperature (45 1C, 20–30 min) and oxidative stress (0.4 mol-¹) hydrogen peroxide, 20–60 min). HS-treated yeast cells showed less accumulation of reactive oxygen species (ROS) than non-treated cells in response to both stresses. Additionally, HS-treated yeast exhibited significantly greater (P≥0.0001) biocontrol activity against Penicillium expansum and a significantly faster (Po0.0001) growth rate in wounds of apple fruits stored at 25 1C compared with the performance of untreated yeast cells. Transcription of a trehalose-6-phosphate synthase gene (TPS1) was up regulated in response to HS and trehalose content also increased.
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