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Статті в журналах з теми "Vivo conditions"

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Автор: Grafiati

Опубліковано: 6 вересня 2023

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1

de Maertelaer, V., and D. Pottier. "Stability conditions of cell populations in vivo." Journal of Theoretical Biology 113, no. 2 (March 1985): 299–310. http://dx.doi.org/10.1016/s0022-5193(85)80229-0.

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2

Pavlova, I. A., and V. P. Klimenko. "MODELING OF THE CLIMATIC CONDITIONS FOR THE ADAPTATION OF GRAPE PLANTS IN VITRO TO CONDITIONS IN VIVO." Scientific Works of North Caucasian Federal Scientific Center of Horticulture, Viticulture, Wine-making 25 (October 2019): 164–68. http://dx.doi.org/10.30679/2587-9847-2019-25-164-168.

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3

Henselová, M., A. Lux, and E. Masarovičová. "Effect of growth regulators on rooting cuttings of Karwinskia species under in vivo conditions." Plant, Soil and Environment 48, No. 10 (December 22, 2011): 471–76. http://dx.doi.org/10.17221/4397-pse.

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Анотація:

Effect of the growth regulators Atonik, Rastim 30 DKV, Stimulator AS 1, and Stimulax III on rooting of half-woody shoots of the species Karwinskia humboldtiana (Roem et Schut) Zucc. and Karwinskia parvifolia Rose was studied. Rooting does not occur without stimulation in these species, after stimulation rhizogenesis takes 14 to 16 weeks. Growth regulators, with the exception of the preparation Atonik, showed a significantly stimulating effect on rhizogenesis, and effect of them declined in the order Stimulax III, Stimulator AS, and Rastim 30 DKV. The percentage of rooting in the species Karwinskia humboldtiana was higher than that in Karwinskia parvifolia and this was dependent on the age of the plants, the type of stimulator, cutting, substrate, and conditions of cultivation.

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4

Zernii, Evgeni Yu, Aliya A. Nazipova, Olga S. Gancharova, Alexey S. Kazakov, Marina V. Serebryakova, Dmitry V. Zinchenko, Natalya K. Tikhomirova, et al. "Light-induced disulfide dimerization of recoverin under ex vivo and in vivo conditions." Free Radical Biology and Medicine 83 (June 2015): 283–95. http://dx.doi.org/10.1016/j.freeradbiomed.2015.03.001.

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5

Gal, D. "Hunt For Singlet Oxygen Under in Vivo Conditions." Biochemical and Biophysical Research Communications 202, no. 1 (July 1994): 10–16. http://dx.doi.org/10.1006/bbrc.1994.1886.

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6

Piesiak-Pańczyszyn, Dagmara, and Urszula Kaczmarek. "Fluoride release from fluoride varnish under in vitro and in vivo conditions." Dental and Medical Problems 54, no. 4 (December 29, 2017): 327–31. http://dx.doi.org/10.17219/dmp/78887.

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7

Oliynyk, O., and M. Melnychuk. "State photosynthetic apparatus of rose essential oil after adaptation to conditions in vivo." Biolohichni systemy 9, no. 2 (December 29, 2017): 187–91. http://dx.doi.org/10.31861/biosystems2017.02.187.

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8

Titenkov, A. V., M. N. Lushpin, T. N. Lushpina, N. V. Kotsareva, and A. N. Kryukov. "Adaptation of microclones of blackberries to in vivo conditions." IOP Conference Series: Earth and Environmental Science 845, no. 1 (November 1, 2021): 012022. http://dx.doi.org/10.1088/1755-1315/845/1/012022.

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Abstract The results of studying the effect of mineral fertilizing on rhizogenesis and the development of aboveground organs of regenerant plants of blackberry thornless adaptable to in vivo conditions in the laboratory of selection, vegetable growing and horticulture, cloning “UNITS” Agrotechnopark “of Belgorod State Agrarian University are presented. Regenerated plants of thornless blackberry cultivar Agavam were adapted to in vivo conditions in a peat-perlite mixture with the addition of microelements and growth regulator root 16 days earlier than in the control. An active growth of the aboveground part and roots of regenerated plants of thornless blackberry was noted on the 21st day, in the control - on the 42nd day after the start of adaptation. By the end of the rooting stage on the 24th day, the regenerant plants formed an aerial part of two pairs of leaves 22 mm high and a developed root system - 37 mm. The mineral and hormonal composition of nutrient media for the cultivation of thornless blackberries has been optimized, an effective combination of physical and chemical factors at different stages of micropropagation has been determined, which enhance the proliferation of shoots and roots, and the dependence of the efficiency of adaptation of regenerated plants to in vivo conditions has been established. Along with traditional breeding methods, new opportunities for solving the problem of thorn-free blackberry varieties are provided, along with traditional breeding methods, which make it possible to accelerate the process of obtaining valuable planting material to provide the population and the processing industry with valuable berry products.

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9

Ran, Shujun, Shensheng Gu, Jia Wang, Cailian Zhu, and Jingping Liang. "Dentin tubule invasion byEnterococcus faecalisunder stress conditions ex vivo." European Journal of Oral Sciences 123, no. 5 (August 21, 2015): 362–68. http://dx.doi.org/10.1111/eos.12202.

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10

Zhu, Chaoyong, Jacob Odeberg, Anders Hamsten, and Per Eriksson. "Allele-specific MMP-3 transcription under in vivo conditions." Biochemical and Biophysical Research Communications 348, no. 3 (September 2006): 1150–56. http://dx.doi.org/10.1016/j.bbrc.2006.07.174.

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11

GIRAEV, K. M., N. A. ASHURBEKOV, and R. T. MEDZHIDOV. "FLUORESCENT-SPECTROSCOPIC RESEARCH OF IN VIVO TISSUES PATHOLOGICAL CONDITIONS." International Journal of Modern Physics B 18, no. 06 (March 10, 2004): 899–910. http://dx.doi.org/10.1142/s0217979204024446.

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The steady-state spectra of autofluorescence and the reflection coefficient on the excitation wavelength of some stomach tissues in vivo with various pathological conditions (surface gastritis, displasia, cancer) are measured under excitation by the nitrogen laser irradiation (λ ex =337.1 nm ). The contour expansion of obtained fluorescence spectra into contributions of components is conducted by the Gaussian–Lorentzian curves method. It is shown that at least 7 groups of fluorophores forming a total luminescence spectrum can be distinguished during the development of displasia and tumor processes. The correlation of intensities of flavins and NAD(P)·H fluorescence is determined and the degree of respiratory activity of cells for the functional condition considered is estimated. The evaluations of the fluorescence quantum yield of the tissue's researched are given.

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12

Maximov, Stanislav, Vera Ott, Lhoussaine Belkoura, and Reinhard Krämer. "Stimulus analysis of BetP activation under in vivo conditions." Biochimica et Biophysica Acta (BBA) - Biomembranes 1838, no. 5 (May 2014): 1288–95. http://dx.doi.org/10.1016/j.bbamem.2013.12.017.

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13

Chung, Yeonseok, Hoyong Lim, Young Uk Kim, Hua Sun, Shino Hanabuchi, and Babie Teng. "Proatherogenic conditions promote autoimmune Th17 responses in vivo (P4136)." Journal of Immunology 190, no. 1_Supplement (May 1, 2013): 191.14. http://dx.doi.org/10.4049/jimmunol.190.supp.191.14.

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Abstract Although patients with atherosclerosis have a higher incidence of systemic autoimmune diseases, the relationship between proatherogenic factors and autoimmune T cell responses is poorly understood. Mice lacking both LDL receptor and apolipoprotein B mRNA editing enzyme (Ldlr-/-Apobec1-/-; LDb) are hyperlipidemic and prone to atherosclerosis. Here, we show that LDb mice exhibit increased IL-17 in circulation as well as in the aortic sinus area, which was attributed to a preferential enhancement of Th17 cells in the secondary lymphoid organs. In addition, the environment within LDb mice was substantially favorable for the Th17 polarization of auto-reactive CD4+ T cells during homeostatic proliferation. In vitro, the addition of oxidized LDL, but not native LDL, promoted dendritic cell-mediated Th17 polarization by triggering IL-6 and IL-1 in a MyD88-dependent fashion. Furthermore, myelin oligodendrocyte glycoprotein (MOG)-reactive CD4+ T cells expanded in the presence of oxidized LDL expressed increased levels of Th17 signature genes, and induced more profound experimental autoimmune encephalitomyelitis (EAE) when transferred into naïve mice. Our findings demonstrate that proatherogenic factors induce the polarization and functional maturation of autoimmune Th17 cells, which may be critical for the pathogenesis of atherosclerosis and other related autoimmune diseases.

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14

Schreiter, Thomas, Guido Marquitan, Malin Darnell, Jan-Peter Sowa, Martina Bröcker-Preuss, Tommy B. Andersson, Hideo A. Baba, et al. "An Ex Vivo Perfusion System Emulating In Vivo Conditions in Noncirrhotic and Cirrhotic Human Liver." Journal of Pharmacology and Experimental Therapeutics 342, no. 3 (June 6, 2012): 730–41. http://dx.doi.org/10.1124/jpet.112.194167.

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15

Kruglova, N. N. "REGULATION OF CEREAL EMBRYONIC ORGANOGENESIS IN VITRO CONDITIONS." ÈKOBIOTEH 4, no. 1 (2021): 11–23. http://dx.doi.org/10.31163/2618-964x-2021-4-1-11-23.

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The article provides the brief review of the literature and own works devoted to the peculiarities of the cereal embryonic organogenesis at the early stages of ontogenesis in the conditions of in vitro culture (the so-called somatic embryogenesis, or embryoidogenesis in vitro). Particular attention is paid to the issues of hormonal regulation of the development of somatic cereal embryos from initial cells to mature structures in vitro. A comparison of somatic embryogenesis in vitro with similar events in zygotic embryogenesis in vivo confirms the validity of the principle of universality of morphogenesis processes in vivo and in vitro (Batygina, 2014). The prospects of using somatic embryogenesis in vitro as a model for studying the most complex biological phenomenon – zygotic plant embryogenesis in vivo – are discussed.

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16

Karpushina, Marina Vladimirovna, and Ivan Ivanovich Suprun. "METHODS AND APPROACHES TO VIRUS ELIMINATION UNDER IN VITRO AND IN VIVO CONDITIONS." Fruit growing and viticulture of South Russia 3, no. 63 (May 15, 2020): 254–69. http://dx.doi.org/10.30679/2219-5335-2020-3-63-254-269.

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17

Lenz, Jan, Frederik Fuest, Jan Henrik Finke, Heike Bunjes, Arno Kwade, and Michael Juhnke. "Tablet Disintegration and Dispersion under In Vivo-like Hydrodynamic Conditions." Pharmaceutics 14, no. 1 (January 16, 2022): 208. http://dx.doi.org/10.3390/pharmaceutics14010208.

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Анотація:

Disintegration and dispersion are functional properties of tablets relevant for the desired API release. The standard disintegration test (SDT) described in different pharmacopoeias provides only limited information on these complex processes. It is considered not to be comparable to the biorelevant conditions due to the frequent occurrence of high hydrodynamic forces, among other reasons. In this study, 3D tomographic laser-induced fluorescence imaging (3D Tomo-LIF) is applied to analyse tablet disintegration and dispersion. Disintegration time (DT) and time-resolved particle size distribution in close proximity to the tablet are determined in a continuously operated flow channel, adjustable to very low fluid velocities. A case study on tablets of different porosity, which are composed of pharmaceutical polymers labelled with a fluorescent dye, a filler, and disintegrants, is presented to demonstrate the functionality and precision of the novel method. DT results from 3D Tomo-LIF are compared with results from the SDT, confirming the analytical limitations of the pharmacopoeial disintegration test. Results from the 3D Tomo-LIF method proved a strong impact of fluid velocity on disintegration and dispersion. Generally, shorter DTs were determined when cross-linked sodium carboxymethly cellulose (NaCMCXL) was used as disintegrant compared to polyvinyl polypyrrolidone (PVPP). Tablets containing Kollidon VA64 were found to disintegrate by surface erosion. The novel method provides an in-depth understanding of the functional behaviour of the tablet material, composition and structural properties under in vivo-like hydrodynamic forces regarding disintegration and the temporal progress of dispersion. We consider the 3D Tomo-LIF in vitro method to be of improved biorelevance in terms of hydrodynamic conditions in the human stomach.

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18

Pratt, George W., and Paul Duchnowski. "Ultrasound method and apparatus for evaluating, in vivo, bone conditions." Journal of the Acoustical Society of America 88, no. 6 (December 1990): 2916. http://dx.doi.org/10.1121/1.399605.

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19

Ujfalussy, Balázs B., Judit K. Makara, Máté Lengyel, and Tiago Branco. "Global and Multiplexed Dendritic Computations under In Vivo-like Conditions." Neuron 100, no. 3 (November 2018): 579–92. http://dx.doi.org/10.1016/j.neuron.2018.08.032.

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20

Weinans, Harrie, and Leendert Blankevoort. "Reconstruction of bone loading conditions from in vivo strain measurements." Journal of Biomechanics 28, no. 6 (June 1995): 739–44. http://dx.doi.org/10.1016/0021-9290(94)00122-k.

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21

Hanton, D. Y., H. Furtak, and H. G. Grimm. "Preparation and Handling Conditions of MMVF for In-Vivo Experiments." Aerosol Science and Technology 29, no. 5 (January 1998): 449–56. http://dx.doi.org/10.1080/02786829808965583.

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22

Van Ginckel, Ans, Fredrik Almqvist, Koenraad Verstraete, Philip Roosen, and Erik Witvrouw. "Human ankle cartilage deformation after different in vivo impact conditions." Knee Surgery, Sports Traumatology, Arthroscopy 19, no. 1 (May 20, 2010): 137–43. http://dx.doi.org/10.1007/s00167-010-1159-4.

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23

Mysliwa-Kurdziel, Beata, Jerzy Kruk, and Kazimierz Strzałka. "Protochlorophyllide in model systems — An approach to in vivo conditions." Biophysical Chemistry 175-176 (May 2013): 28–38. http://dx.doi.org/10.1016/j.bpc.2013.02.002.

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24

Singh, Amit, Surendra Mishra, and Abha Mishra. "Comparative study of assay of free radical damage in in vivo, in vitro and ex vivo conditions." International Journal of Basic & Clinical Pharmacology 3, no. 2 (2014): 308. http://dx.doi.org/10.5455/2319-2003.ijbcp20140411.

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25

Malik, Faizan A., Bradley A. Drahos, Amer M. Safdari, Mark V. Mazzeo, Jack E. Norfleet, Robert M. Sweet, and Timothy M. Kowalewski. "Variability of tissue mechanical response in Sus Domesticus porcine models from in vivo to ex vivo conditions." PLOS ONE 18, no. 5 (May 10, 2023): e0268608. http://dx.doi.org/10.1371/journal.pone.0268608.

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Background Healthcare simulators have been demonstrated to be a valuable resource for training several technical and nontechnical skills. A gap in the fidelity of tissues has been acknowledged as a barrier to application for current simulators; especially for interventional procedures. Inaccurate or unrealistic mechanical response of a simulated tissue to a given surgical tool motion may result in negative training transfer and/or prevents the “suspension of disbelief” necessary for a trainee to engage in the activity. Thus, where it is relevant to training outcomes, there should be an effort to create healthcare simulators with simulated tissue mechanical responses that match or represent those of biological tissues. Historically, this data is most often gathered from preserved (post mortem) tissue; however, there is a concern that the mechanical properties of preserved tissue, that lacks blood flow, may lack adequate accuracy to provide the necessary training efficacy of simulators. Methods and findings This work explores the effect of the “state” of the tissue testing status on liver and peritoneal tissue by using a customized handheld grasper to measure the mechanical responses of representative porcine (Sus domesticus) tissues in n = 5 animals across five test conditions: in vivo, post mortem (in-situ), ex vivo (immediately removed from fresh porcine cadaver), post-refrigeration, and post-freeze-thaw cycle spanning up to 72 hours after death. No statistically significant difference was observed in the mechanical responses due to grasping between in vivo and post-freeze conditions for porcine liver and peritoneum tissue samples (p = 0.05 for derived stiffness at grasping force values F = 5N and 6.5N). Furthermore, variance between in vivo and post-freeze conditions within each animal, was comparable to the variance of the in vivo condition between animals. Conclusions Results of this study further validate the use of preserved tissue in the design of medical simulators via observing tissue mechanical responses of post-freeze tissue comparable to in vivo tissue. Therefore, the use of thawed preserved tissue for the further study and emulation of mechanical perturbation of the liver and peritoneum can be considered. Further work in this area should investigate these trends further, particularly in regard to other tissues and the potential effects varying preservation methods may yield.

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26

Lipatov, V. A., S. V. Lazarenko, A. N. Betz, and D. A. Severinov. "Changes of Physico-Mechanical Properties of Vascular Patches in Conditions of Chronic Experiment in vivo." Novosti Khirurgii 27, no. 3 (June 25, 2019): 249–55. http://dx.doi.org/10.18484/2305-0047.2019.3.249.

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27

Elkonin, L. A., S. N. Timofeeva, and O. I. Yudakova. "Breaking Physical Dormancy of the Laburnum anagyroides Seeds by in vivo and in vitro Conditions." Chemistry. Biology. Ecology 17, no. 1 (2017): 30–35. http://dx.doi.org/10.18500/1816-9775-2017-17-1-30-35.

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28

Siminski, J. T., T. J. Kavanagh, E. Chi, and G. Raghu. "Long-term maintenance of mature pulmonary parenchyma cultured in serum-free conditions." American Journal of Physiology-Lung Cellular and Molecular Physiology 262, no. 1 (January 1, 1992): L105—L110. http://dx.doi.org/10.1152/ajplung.1992.262.1.l105.

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Анотація:

Current concepts of the pathogenesis of lung injury and repair are derived from in vitro cellular and in vivo investigations. Studies with viable ex vivo models may offer additional insights into disease processes, since essential cellular interactions would be maintained. However, a major limiting factor has been the availability of a model that maintains normal parenchymal structure, viability, and homeostasis beyond 4 wk in serum-free conditions. We have succeeded in establishing an ex vivo lung culture system which reproducibly maintains parenchymal architecture for up to 9 wk. Our method is a simple, modified version of previously utilized techniques. Thin slices of mature murine lung were inflated with agar-defined medium and cultured on Gelfoam saturated with serum-free medium. Normal pulmonary parenchyma, with the exception of endothelial cells, was maintained for up to 60 days as assessed chronologically by light and electron microscopy. The integrity of the microvasculature and endothelial cells was lost beyond 7 days. The adult lung ex vivo culture system maintained necessary epithelial and interstitial cellular interactions in the alveolar wall without systemic circulatory influences. Future studies with this model may provide important insights in assessing the pathogenesis of many acute and chronic lung diseases and clarify existing controversies raised from in vitro and in vivo studies.

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29

Beliveau Carey, G., C. W. Cheung, N. S. Cohen, S. Brusilow, and L. Raijman. "Regulation of urea and citrulline synthesis under physiological conditions." Biochemical Journal 292, no. 1 (May 15, 1993): 241–47. http://dx.doi.org/10.1042/bj2920241.

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Information on the regulation of urea synthesis in vivo was obtained by examining the relationship between ureagenesis in vivo, citrulline synthesis in vitro, and two factors currently hypothesized to exert short-term regulation of this pathway: the liver mitochondrial content of N-acetylglutamate (NAG) and substrate availability. Rats meal-fed for 4 h every day (4-20 schedule) or for 8 h every other day (8-40 schedule) were used. (1) The citrulline-synthesizing capacity of mitochondria from livers of rats on the 8-40 schedule exceeded the corresponding velocity of urea synthesis in vivo at all time points studied. (2) Mitochondrial NAG in these livers increased from 127 +/- 32 pmol/mg of protein at 0 h to 486 +/- 205 pmol/mg at 3 h after the start of a meal, and decreased thereafter, but the correlation between NAG content and the velocity of citrulline synthesis was not simple, suggesting that NAG is not the only determinant of the state of activation of carbamoyl phosphate synthase I. (3) In rats on the 4-20 schedule killed 1 h after the start of the meal, the liver content of ornithine, citrulline, arginine, glutamate, alanine and urea increased 2.1-12-fold with respect to the values at 0 h; glutamine decreased by 39%. (4) The combined findings indicate that in vivo, moment-to-moment control of the velocity of urea synthesis is exerted by substrate availability. (5) Digestion limits the supply of substrate to the liver, and prevents its ureagenic capacity from being overwhelmed following a protein-containing meal.

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30

Ercan-Fang, Nacide G., Frank Q. Nuttall, and Mary C. Gannon. "Uric acid inhibits liver phosphorylase aactivity under simulated in vivo conditions." American Journal of Physiology-Endocrinology and Metabolism 280, no. 2 (February 1, 2001): E248—E253. http://dx.doi.org/10.1152/ajpendo.2001.280.2.e248.

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We have reported that glycogen synthesis and degradation can occur in vivo without a significant change in the amount of phosphorylase a present. These data suggest the presence of a regulatable mechanism for inhibiting phosphorylase a activity in vivo. Several effectors have been described. AMP stimulates, whereas ADP, ATP, and glucose inhibit activity. Of these effectors, only the glucose concentration changes under normal conditions; thus it could regulate phosphorylase a activity in vivo. We previously have reported that, when all of these effectors were present at physiological concentrations, the net effect was no change in phosphorylase a activity. Addition of caffeine, an independent inhibitor of activity, to the above effectors not only resulted in inhibition but also restored a glucose concentration-dependent inhibition. Because uric acid is an endogenous xanthine derivative, we decided to determine whether it had an effect on phosphorylase a activity. Independently, uric acid did not affect activity; however, when added at a presumed physiological concentration in combination with AMP, ADP, ATP, and glucose, it inhibited activity. A modest but not statistically significant glucose concentration-dependent inhibition was also present. Thus uric acid may play an important role in regulating phosphorylase a activity in vivo.

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31

GARLET, Gustavo P., and Carlos F. SANTOS. "Cell culture conditions: from outer space-like conditions to the mimicking of complex in vivo environments." Journal of Applied Oral Science 22, no. 3 (June 2014): 144–45. http://dx.doi.org/10.1590/1678-77572014ed003.

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32

Richter, Diethelm W., and Jeffrey C. Smith. "Respiratory Rhythm Generation In Vivo." Physiology 29, no. 1 (January 2014): 58–71. http://dx.doi.org/10.1152/physiol.00035.2013.

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The cellular and circuit mechanisms generating the rhythm of breathing in mammals have been under intense investigation for decades. Here, we try to integrate the key discoveries into an updated description of the basic neural processes generating respiratory rhythm under in vivo conditions.

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33

Simon, Johanna, Gabor Kuhn, Michael Fichter, Stephan Gehring, Katharina Landfester, and Volker Mailänder. "Unraveling the In Vivo Protein Corona." Cells 10, no. 1 (January 12, 2021): 132. http://dx.doi.org/10.3390/cells10010132.

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Understanding the behavior of nanoparticles upon contact with a physiological environment is of urgent need in order to improve their properties for a successful therapeutic application. Most commonly, the interaction of nanoparticles with plasma proteins are studied under in vitro conditions. However, this has been shown to not reflect the complex situation after in vivo administration. Therefore, here we focused on the investigation of magnetic nanoparticles with blood proteins under in vivo conditions. Importantly, we observed a radically different proteome in vivo in comparison to the in vitro situation underlining the significance of in vivo protein corona studies. Next to this, we found that the in vivo corona profile does not significantly change over time. To mimic the in vivo situation, we established an approach, which we termed “ex vivo” as it uses whole blood freshly prepared from an animal. Overall, we present a comprehensive analysis focusing on the interaction between nanoparticles and blood proteins under in vivo conditions and how to mimic this situation with our ex vivo approach. This knowledge is needed to characterize the true biological identity of nanoparticles.

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34

Feng, Deng-Min, Chen-Xia He, Chao-Yu Miao, Bin Lu, Wen-Jun Wu, You-Fa Ding, and Jing-Lun Xue. "Conditions affecting hydrodynamics-based gene delivery into mouse liver in vivo." Clinical and Experimental Pharmacology and Physiology 31, no. 12 (December 2004): 850–55. http://dx.doi.org/10.1111/j.1440-1681.2004.04125.x.

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35

Ostapenko, O. V., A. S. Boitsov, A. N. Baranov, E. A. Lesina, V. M. Mikhailov, and V. S. Baranov. "Influence of Electroporation Conditions on Transfection of Muscle Fibers in Vivo." Russian Journal of Genetics 40, no. 1 (January 2004): 33–39. http://dx.doi.org/10.1023/b:ruge.0000013446.86631.fd.

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36

Naturil-Alfonso, Carmen, María dels Desamparats Saenz-de-Juano, David S. Peñaranda, José S. Vicente, and Francisco Marco-Jiménez. "Transcriptome Profiling of Rabbit Parthenogenetic Blastocysts Developed under In Vivo Conditions." PLoS ONE 7, no. 12 (December 12, 2012): e51271. http://dx.doi.org/10.1371/journal.pone.0051271.

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Ursem, Stan R., Marc G. Vervloet, Jacquelien J. G. Hillebrand, Renate T. de Jongh, and Annemieke C. Heijboer. "Oxidation of PTH: in vivo feature or effect of preanalytical conditions?" Clinical Chemistry and Laboratory Medicine (CCLM) 56, no. 2 (January 26, 2018): 249–55. http://dx.doi.org/10.1515/cclm-2017-0313.

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Abstract Background: Posttranslational oxidation of parathyroid hormone (PTH) modifies its biological activity. Measurement of non-oxidized PTH (n-oxPTH) could be an improvement in assessing PTH status, as intact PTH may rather reflect oxidative stress. However, it is debated whether oxidation of PTH occurs in vivo, or whether it is mainly an in vitro artifact. The aim of this study was to investigate the influence of different preanalytical conditions on the oxidation of PTH within a wide range of plasma PTH concentrations and oxidation propensity. Methods: n-oxPTH was separated from its oxidized form using an affinity column capturing the oxidized PTH. n-oxPTH was measured in eluate using commercially available PTH assays. The study included ethylenediaminetetraacetic acid plasma samples from 17 patients undergoing hemodialysis and 32 healthy subjects. We determined effects of storage temperature, time until centrifugation and freeze-thaw cycles. PTH and n-oxPTH concentrations were measured in each sample using six different immunoassays. Results: n-oxPTH concentrations remained unchanged up to 180 min until centrifugation, two freeze-thaw cycles or after storage at −20°C or −80°C up to 79 days. Various methods for n-oxPTH and PTH measurements yielded highly comparable results, apart from standardization differences between various PTH and n-oxPTH assays. Conclusions: n-oxPTH concentrations were stable under our study conditions, indicating negligible ex vivo oxidation of PTH. In addition, PTH immunoassays have a different sensitivity for n-oxPTH than for total PTH. For this reason, the n-oxPTH/total PTH ratio cannot be used in absence of a n-oxPTH standard. Clinical implications of determining n-oxPTH require additional study.

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Lussi, A. S., and W. B. Buergin. "A New to the Condensation of Amalgam Under in vivo Conditions." Journal of Dental Research 66, no. 3 (March 1987): 737–39. http://dx.doi.org/10.1177/00220345870660030601.

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Agostinis, Chiara, Stefania Biffi, Chiara Garrovo, Paolo Durigutto, Andrea Lorenzon, Alpan Bek, Roberta Bulla та ін. "In vivo distribution of β2 glycoprotein I under various pathophysiologic conditions". Blood 118, № 15 (13 жовтня 2011): 4231–38. http://dx.doi.org/10.1182/blood-2011-01-333617.

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Abstract In vitro studies have documented β2 glycoprotein I (β2GPI) binding to endothelial cells (ECs) and trophoblast using antiphospholipid antibodies. The in vivo binding of β2GPI to these cells and the conditions that favor their interaction have not been investigated. We analyzed the in vivo distribution of cyanine 5.5-labeled β2GPI in mice and evaluated the effect of pregnancy and circulating antibodies on its tissue localization. The signal was detected in the liver by whole body scan and ex vivo analysis. The β2GPI failed to bind to the vascular endothelium and reacted only with the ECs of uterine vessels. In pregnant mice the protein was localized on ECs and trophoblast at the embryo implantation sites. Immunized mice showed a similar β2GPI biodistribution to naive mice but the immunized pregnant animals exhibited a significant increase in fetal loss associated with C3 and C9 deposition at the implantation sites. Treatment of mice with LPS after β2GPI-Cy5.5 injection promoted protein localization on gut and brain ECs associated with IgG, C1q, and C9 deposition in immunized mice. These findings indicate that β2GPI binding to EC requires priming with pro-inflammatory factors which is not needed for uterine and placental localization probably dependent on hormonal changes.

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40

Whitcher, F. D. "Simulation of in vivo loading conditions of nitinol vascular stent structures." Computers & Structures 64, no. 5-6 (September 1997): 1005–11. http://dx.doi.org/10.1016/s0045-7949(97)00014-x.

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41

Schmid, V., R. J. Egli, A. Tekari, R. Luginbuehl, and V. Schmid. "467 A PHYSIOLOGIC ROBOT REACTOR SYSTEM TO SIMULATE IN VIVO CONDITIONS." Osteoarthritis and Cartilage 19 (September 2011): S217. http://dx.doi.org/10.1016/s1063-4584(11)60494-9.

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42

Lim, Hoyong, Young Uk Kim, Hua Sun, Joyce H. Lee, Joseph M. Reynolds, Shino Hanabuchi, Huaizhu Wu, Ba-Bie Teng, and Yeonseok Chung. "Proatherogenic Conditions Promote Autoimmune T Helper 17 Cell Responses In Vivo." Immunity 40, no. 1 (January 2014): 153–65. http://dx.doi.org/10.1016/j.immuni.2013.11.021.

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43

González-Correa, C. A., B. H. Brown, R. H. Smallwood, N. Kalia, C. J. Stoddard, T. J. Stephenson, S. J. Haggie, D. N. Slater, and K. D. Bardhan. "Assessing the conditions forin vivo electrical virtual biopsies in Barrett's oesophagus." Medical & Biological Engineering & Computing 38, no. 4 (July 2000): 373–76. http://dx.doi.org/10.1007/bf02345004.

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44

Klich, Maren A., Sean Tang, and David W. Denning. "Aflatoxin and Ochratoxin Production by Aspergillus Species Under Ex Vivo Conditions." Mycopathologia 168, no. 4 (June 19, 2009): 185–91. http://dx.doi.org/10.1007/s11046-009-9215-7.

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45

SRIRAMARAO, P., and DAVID H. BROIDE. "Differential Regulation of Eosinophil Adhesion under Conditions of Flow In Vivo." Annals of the New York Academy of Sciences 796, no. 1 Cytokines and (October 1996): 218–25. http://dx.doi.org/10.1111/j.1749-6632.1996.tb32584.x.

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46

Orly, I., M. Gregoire, J. Menanteau, M. Heughebaert, and B. Kerebel. "Chemical changes in hydroxyapatite biomaterial underin vivo andin vitro biological conditions." Calcified Tissue International 45, no. 1 (January 1989): 20–26. http://dx.doi.org/10.1007/bf02556656.

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47

Moussavi-Biugui, Amir, Bram Stieltjes, Klaus Fritzsche, Wolfhard Semmler, and Frederik B. Laun. "Novel spherical phantoms for Q-ball imaging under in vivo conditions." Magnetic Resonance in Medicine 65, no. 1 (August 25, 2010): 190–94. http://dx.doi.org/10.1002/mrm.22602.

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48

Sonck, Kathleen A. J., Gwendoline Kint, Geert Schoofs, Corinne Vander Wauven, Jos Vanderleyden, and Sigrid C. J. De Keersmaecker. "The proteome of Salmonella Typhimurium grown under in vivo-mimicking conditions." PROTEOMICS 9, no. 3 (February 2009): 565–79. http://dx.doi.org/10.1002/pmic.200700476.

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49

Garcia, Pio. "Computer simulations and experiments: in vivo–in vitro conditions in biochemistry." Foundations of Chemistry 17, no. 1 (March 22, 2015): 49–65. http://dx.doi.org/10.1007/s10698-015-9215-2.

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50

Tian, Chenglei, Jing Wang, Xiaoying Ye, Jiyu Chen, Rongyan Zheng, Hanwen Yu, Jie Li, et al. "Culture conditions of mouse ESCs impact the tumor appearance in vivo." Cell Reports 42, no. 6 (June 2023): 112645. http://dx.doi.org/10.1016/j.celrep.2023.112645.

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