Дисертації з теми "Vivo conditions"
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся з топ-50 дисертацій для дослідження на тему "Vivo conditions".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Переглядайте дисертації для різних дисциплін та оформлюйте правильно вашу бібліографію.
Haque, Sameena. "In Vivo Imaging of Corneal Conditions using Optical Coherence Tomography." Thesis, University of Waterloo, 2006. http://hdl.handle.net/10012/2976.
Повний текст джерелаMethods: Topographical thickness of the cornea was measured using OCT in all studies. The CRTTM studies investigated myopic and hyperopic treatment, throughout the day. The myopic studies followed lens wear over a 4 week period, which was extended to 12 months, and investigated the thickness changes produced by two lenses of different oxygen transmissibility. CRTTM for hyperopia (CRTHTM) was evaluated after a single night of lens wear.
In the investigation of keratoconus, OCT corneal thickness values were compared to those obtained from Orbscan II (ORB) and ultrasound pachymetry (UP). A new fixation device was constructed to aid in the measurement of topographical corneal and epithelial thickness along 8 directions of gaze. Pachymetry maps were produced for the normal non-lens wearing cornea, and compared with the rigid gas permeable (RGP) lens wearing cornea and the keratoconic cornea.
Thickness changes prior to, and following LASIK were measured and monitored throughout six months. Myopic and hyperopic correction was investigated individually, as the laser ablation profiles differ for each type of procedure. The LASIK flap interface was also evaluated by using light backscatter data to monitor healing.
Results: Following immediate lens removal after myopic CRTTM, the central cornea swelled less than the periphery, with corneal swelling recovering to baseline levels within 3 hours. The central epithelium decreased and mid-peripheral epithelium increased in thickness, with a more gradual recovery throughout the day. There also seemed to be an adaptation effect on the cornea and epithelium, showing a reduced amount of change by the end of the 4 week study period. The thickness changes did not alter dramatically during the 12 month extended study. In comparing the two lens materials used for myopic CRTTM (Dk/t 91 vs. 47), there were differences in stromal swelling, but no differences in the central epithelial thinning caused by lens wear. There was a statistically insignificant asymmetry in mid-peripheral epithelial thickening between eyes, with the lens of lower Dk causing the greater amount of thickening. Hyperopic CRTTM produced a greater increase in central stromal and central epithelial thickness than the mid-periphery. Once again, the stroma recovered faster than the epithelium, which remained significantly thicker centrally for at least six hours following lens removal.
Global pachymetry measurements of the normal cornea and epithelium found the periphery to be thicker than the centre. The superior cornea and epithelium was thicker than the inferior. In the measurement of the keratoconic cornea, OCT and ORB correlated well in corneal thickness values. UP measured greater values of corneal thickness. The keratoconic epithelium was thinner than normal, and more so over the apex of the cone than at the centre. The location of the cone was most commonly found in the inferior temporal region. Central epithelial thickness was thinner in keratoconics than in RGP lens wearers, which in turn was thinner than in non-lens wearers.
Following LASIK surgery for both myopia and hyperopia, the topographical OCT thickness profiles showed stromal thinning in the areas of ablation. The central myopic cornea showed slight regression at 6 months. During early recovery, epithelial thickness increased centrally in hyperopes and mid-peripherally in myopes. By the end of the 6 month study, mid-peripheral epithelial thickness was greater than the centre in both groups of subjects. The light backscatter profiles after LASIK showed a greater increase in backscatter on the anterior side of the flap interface (nearer the epithelium), than the posterior side (in the mid-stroma) during healing. The flap interface was difficult to locate in the OCT images at 6 months.
Conclusion: All the CRTTM lenses used in this project produced more corneal swelling than that seen normally overnight without lens wear. In order for these lenses to be worn safely for long periods of time without affecting the health of the cornea, they need to be manufactured from the highest oxygen transmissible material available. The long-term effect of thinning on the epithelium's barrier properties needs to be monitored closely.
Global topographical thickness of the cornea and epithelium was measured using OCT in normal, RGP lens wearing and keratoconic eyes. Corneal and epithelial thickness was not symmetrical across meridians. The epithelium of RGP lens wearers was slightly thinner than normal, but not as thin as in keratoconics, suggesting that the epithelial change seen in keratoconus is mainly due to the condition.
Post-LASIK corneal and epithelial thickness profiles were not the same for myopic and hyperopic subjects, since the ablation patterns vary. Epithelial thickening in the mid-periphery had not recovered by six months in myopes or hyperopes, possibly indicating epithelial hyperplasia. Light backscatter profiles were used to monitor the recovery of the LASIK flap interface, showing the band of light backscatter around the flap interface to decrease as the cornea healed.
Persson, Eva. "Drug Dissolution under Physiologically Relevant Conditions In Vitro and In Vivo." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Universitetsbiblioteket [distributör], 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7195.
Повний текст джерелаCarruthers, Michael D. "Transcriptional analysis of Escherichia coli O157:H7 during in vivo mimicking conditions." [Ames, Iowa : Iowa State University], 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3389094.
Повний текст джерелаWei, Hongjiang. "In vivo diffusion tensor imaging (DTI) for the human heart under free-breathing conditions." Thesis, Lyon, INSA, 2013. http://www.theses.fr/2013ISAL0127/document.
Повний текст джерелаThe orientation of cardiac fibers underlies the electro-mechanical behavior of the heart, and it is known to be altered in various cardiac diseases such as ischemic heart disease and ventricular hypertrophy. This thesis mainly focuses on in vivo diffusion tensor imaging (DTI) to obtain the myocardial fiber structure of the human heart under free-breathing conditions. The use of DTI for studying the human heart in vivo is challenging due to cardiac motion. In particular, free-breathing DTI acquisition without resorting to respiratory gating is very difficult due to both respiratory and cardiac motion. To deal with this problem, we propose novel approaches that combine multiple shifted trigger delay (TD) acquisitions and post-processing methods. First, we perform multiple shifted TD acquisitions at end diastole. Then, we focus on two different post-processing methods. The first method addresses physiological motion effects on in vivo cardiac DTI using image co-registration and PCATMIP (Principal Components Analysis filtering and Temporal Maximum Intensity Projection). The second method is a wavelet-based image fusion (WIF) algorithm combined with a PCA noise removing method. Finally, a comparison of DTI measurements between the PCATMIP and WIF methods is also performed; tensor fields are calculated, from which the in vivo fiber architecture properties are compared. The results show that using the proposed approaches, we are able to study the cardiac motion effects on diffusion tensor parameters, and investigate the underlying relationship between the measured diffusion tensor properties and the cardiac motion. We also find that the combination of multiple shifted TD acquisitions and dedicated image post-processing can compensate for physiological motion effects, which allows us to obtain 3D fiber architectures of the human heart under free-breathing conditions. The findings suggest new solutions to signal loss problems associated with bulk motion, which are promising for obtaining in vivo human myocardial fiber architecture properties in clinical conditions
Rovery, Clarisse. "Etude de la transcription de Rickettsia conorii dans différentes conditions in vitro et in vivo." Aix-Marseille 2, 2009. http://www.theses.fr/2009AIX20668.
Повний текст джерелаMittag, Manuel [Verfasser]. "Functional in vivo calcium imaging in the hippocampus under healthy and disease conditions / Manuel Mittag." Bonn : Universitäts- und Landesbibliothek Bonn, 2020. http://d-nb.info/1208937480/34.
Повний текст джерелаPirot, Nelly. "Conséquences in vivo de l'absence de Lyl sur la fonction endothéliale en conditions physiologique et pathologique." Montpellier 2, 2009. http://www.theses.fr/2009MON20100.
Повний текст джерелаThe transcriptional factor LYL, belonging to the bHLH family, is expressed in developing hematopoietic and vascular systems. Lyl-deficient mice are viable and display a reduced number of mature B cells and a diminution in the frequency of immature progenitors. Up to now, nothing was known concerning the role of LYL in endothelial cell. The results presented in this thesis demonstrated that Lyl is expressed both in angiogenic and quiescent endothelium. In vivo, deletion of Lyl increases angiogenesis processes observed in syngenic tumors and in matrigel plugs subcutaneously implanted in mice. Tumor blood vessels from Lyl-deficient mice are larger, leakier and more immature. This phenotype of newly formed vessels is associated with a sustained expression of Tal-1 and an increased expression of Ang-2 and VE-cadherin. In vitro, LYL invalidation by siRNA in HUVEC induces the reduction of the expression of genes regulating the formation of adherens junctions and the binding to extracellular matrix. All together, these results demonstrate that Lyl is involved in the initiation and the maintenance of blood vessels stabilization and maturation. Furthermore, the study of the vascularized organs in adult mice showed the presence, in the lungs of Lyl-deficient mice, of cellular infiltrates composed of inflammatory cells and associated with an increased permeability of the endothelium. Therefore, Lyl might also be involved in the maintenance of endothelial barrier in the lung. This work establishes for the first time the importance of Lyl in the endothelial cell physiology and opens news ways to study the regulation of angiogenesis and the control of vascular permeability
Rodriguez, Alejandro. "Studies of Stroma Formation and Regulation in Human Pathological Conditions and in Experimental in vivo Models." Doctoral thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-120688.
Повний текст джерелаHoon, QiCai Jason. "Fracture biomechanics of screw-hole defects under various loading conditions – An ex-vivo feline femoral model." Thesis, The University of Sydney, 2021. https://hdl.handle.net/2123/26243.
Повний текст джерелаHristovska, Ines. "Dynamique microgliale en conditions physiologiques : un mécanisme contrôlé par les états de vigilance et l’activité neuronale." Thesis, Lyon, 2019. https://n2t.net/ark:/47881/m60c4v3q.
Повний текст джерелаMicroglia, the resident immune cells of the central nervous system (CNS), were traditionally believed to be set into action only by injury or diseases. Strikingly, in the healthy brain, microglia actively carry out parenchyma patrolling by extending and retracting their ramified processes. These movements are referred to as microglial motility and may be to some extent directed toward synapses. However, motility regulation and the purpose of microglia-spine contacts remain elusive. We thus examined the influence of neuronal activity on microglial motility, morphology and microglia-spine interactions during sleep and wakefulness. We found that microglial motility and morphology are modulated by vigilance states. Microglial processes were found to be attracted by active synapses particularly during wake, whereas sleep downregulates microglial proximity and activity-dependent contact with spines. Microglial contact resulted in increased spine activity which was mainly observed during sleep. Understanding the mechanisms regulating microglial dynamics and microglia-spine interactions across the vigilance states will provide further insights into how microglial cells may be involved in sleep- associated functions such as synaptic homeostasis, learning and memory. Grasping these cellular interactions in physiological conditions is crucial to understand synaptic functioning and alterations when microglia are engaged into their immune functions, a hallmark of most brain pathologies
Cozzolino, Olga. "Two-photon imaging with fluorescent biosensors to study neuronal activity in vivo in physiological and pathological conditions." Doctoral thesis, Scuola Normale Superiore, 2019. http://hdl.handle.net/11384/85930.
Повний текст джерелаSchramm, Adrien. "Impact de conductances synaptiques et intrinsèques sur les propriétés de décharge de neurones corticaux in vivo en conditions artificielles et fonctionnelles." Paris 6, 2010. http://www.theses.fr/2010PA066092.
Повний текст джерелаToews, Judy. "Mass spectrometric indentification of formaldehyde-induced modifications of peptides and proteins under in vivo protein cross-linking conditions." Thesis, University of British Columbia, 2008. http://hdl.handle.net/2429/921.
Повний текст джерелаDuong, Sun. "Evaluation of novel fluorescent probes for in vivo Transthyretin amyloid using fibrils generated in vitro under varying conditions." Thesis, Linköpings universitet, Institutionen för fysik, kemi och biologi, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-154611.
Повний текст джерелаMorizane, Asuka. "Optimal conditions for in vivo induction of dopaminergic neurons from embryonic stem cells through cell-derived inducing activity." Kyoto University, 2004. http://hdl.handle.net/2433/147450.
Повний текст джерелаBUFFOLO, FEDERICA. "In vitro and in vivo characterization of the RE-1 Silencing Transcription Factor (REST) activity under neuroinflammatory conditions." Doctoral thesis, Università degli studi di Genova, 2019. http://hdl.handle.net/11567/939868.
Повний текст джерелаSmithpeter, Colin Lee. "Fiber optic confocal imaging for in vivo detection and diagnosis of pre-cancerous lesions /." Digital version accessible at:, 1997. http://wwwlib.umi.com/cr/utexas/main.
Повний текст джерелаBudidha, K. "In vivo investigations of photoplethysmograms and arterial oxygen saturation from the auditory canal in conditions of compromised peripheral perfusion." Thesis, City, University of London, 2016. http://openaccess.city.ac.uk/16134/.
Повний текст джерелаCarletti, Renzo. "Characteristics of NMDA receptor ligands in rodent brain, in vivo and in vitro, under normal conditions and after chronic ischaemia." Thesis, University College London (University of London), 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243989.
Повний текст джерелаKarray, Sahar. "Etude écotoxicologique et phylogéographique de la coque Cerastoderma glaucum issue du Golfe de Gabès : réponse adaptative (in situ et in vivo) au stress métallique et structure génétique." Thesis, Le Mans, 2015. http://www.theses.fr/2015LEMA1007/document.
Повний текст джерелаCerastoderma glaucum is an adequate biological model for phylogeographic and ecotoxicological studies.A study of its genetic structure in the Western Mediterranean sea was conducted using two markers types: mitochondrial and nuclear. The DNA mitochondrial marker results show a divergent population from all the other ones (Ellouza South ofTunisia).An ecotoxicological study of populations sampled from Ellouza site and two other nearby sites in the Gulf of Gabes region was carried out in order to study the adaptive response of natural populations of C. glaucum at different levels of biological organization from the individual level to the cellular and molecular ones. In parallel, two laboratory experiments were performed to clarify the mechanisms that may explain the success or failure of maintaining populations of C. glaucum. In thefirst one, industrial effluents were used and in the second we have used a pure contaminant, the cadmium. These studies experiments concerned individual and molecular levels. Different biomarkers involved in the response to different types of stress: (MT), (ABCB1) (MnSOD and CuZnSOD) (CAT) (HSP70) and (COI) were used in natural and exposed cockles.Results showed the importance of energy metabolism in response to stress whatever the used approach (in situ / Experimental) through the regulation of the expression of the COI gene
Pagès-Hélary, Sandy. "Libération en bouche des molécules de la flaveur : influence des composés salivaires au niveau macroscopique et moléculaire." Thesis, Dijon, 2014. http://www.theses.fr/2014DIJOS055/document.
Повний текст джерелаThe aim of this work is to give a deeper understanding of the impact of the salivary composition on aroma release, by two approaches, an in vitro and an in vivo approach. The impact of salivary proteins on the release of 10 aroma compounds (5 esters and 5 ketones, varying in their hydrophobic chain length) was first investigated by in vitro model systems composed of artificial and human saliva. Artificial salivas were composed of the main salivary proteins, mucins and alpha amylase, alone and in mixture.The amount of aroma released in the vapor phase at equilibrium was analyzed by Static Headspace Gas Chromatography analysis. Air/system partition coefficients have been calculated. This in vitro approach allowed us to demonstrate the ability of both mucin and alpha-amylase to decrease the release of aroma compounds by hydrophobic effect (increase of retention with aroma hydrophobicity). Interestingly, no cumulative effect was observed when both proteins were mixed together in solution. The release of ketones in presence of human saliva is lower than in water and slightly higher than in the presence of artificial saliva. Esters are more affected by the presence of human saliva than ketones. This observation is due to an esterase activity of saliva, which increases with the hydrophobicity of esters. The in vivo release of ethyl propanoate and ethyl hexanoate was followed on ten subjects by Atmospheric Pressure Chemical Ionization mass spectrometry (APCI-MS) under different physiological conditions: at rest, after stimulation and after removing the superficial salivary coat. The saliva was characterized for each subject and each physiological condition. Great variations were observed between the subjects on the salivary flow, viscosity, composition and for each subject between the physiological conditions. The differences observed on in vivo release parameters are discussed as a function of physiological parameters. We observed that subjects with a more viscous saliva present a lower amount of aroma released. The presence of higher amounts of alpha-amylase increased the time needed to release the more hydrophobic compound, ethyl hexanoate. Our results suggest that the retention of hydrophobic aroma compounds by salivary proteins induces a modification of the kinetics of aroma release in real consumption conditions, and could be responsible for aroma persistence
GASPARD, MARIE. "Conditions de realisation de 222 lymphaphereses chez 32 adultes et 9 enfants lors de traitement par interleukine 2 in vivo et cellules lak." Lyon 1, 1989. http://www.theses.fr/1989LYO1M435.
Повний текст джерелаDe, Vaal Michael Hamman. "In vivo mechanical loading conditions of pectorally implanted cardiac pacemakers : feasibility of a force measurement system and concept of an animal-human transfer function." Master's thesis, University of Cape Town, 2009. http://hdl.handle.net/11427/2776.
Повний текст джерелаIncludes bibliographical references (leaves 92-98).
The objectives of this study were to assess the feasibility of this system for measuring in vivo mechanical loading on pectorally implanted pacemakers, to compare differences in mechanical loading experienced by pacemakers in different pectoral implant positions (i.e. sub-muscular and sub-coetaneous) and to formulate a concept of an interspecific transfer function for predicting the in vivo mechanical loads on sub-muscularly implanted pacemakers in humans, by using data obtained from baboons.
Valero, Freitag Susana [Verfasser], and Nikolaus [Akademischer Betreuer] Plesnila. "Local and remote effects of pathological conditions on pyramidal neurites : a longitudinal study using in vivo two-photon microscopy / Susana Valero Freitag ; Betreuer: Nikolaus Plesnila." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2020. http://d-nb.info/1220631930/34.
Повний текст джерелаFéron, François. "Nez, neurones et neurogenèse : analyse in vivo et in vitro des conditions permettant de reproduire le processus de neurogenèse observé dans l'épithélium olfactif de rongeurs adultes." Lyon 1, 1995. http://www.theses.fr/1995LYO10270.
Повний текст джерелаChu, Qingjun. "S-allylcysteine (SAC) and S-allylmercaptocysteine (SAMC), water soluble garlic derivatives, suppress growth and invasion of androgen-independent prostate cancer, under in vitro and in vivo conditions." View the Table of Contents & Abstract, 2006. http://sunzi.lib.hku.hk/hkuto/record/B36749540.
Повний текст джерелаChu, Qingjun, and 褚慶軍. "S-allylcysteine (SAC) and S-allylmercaptocysteine (SAMC), water soluble garlic derivatives, suppress growth and invasion of androgen-independent prostate cancer, under in vitro and in vivo conditions." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B37935653.
Повний текст джерелаJain, Arun Kumar [Verfasser]. "Gastrointestinal transit and erosion behavior of gel matrix tablets: Influence of hydroxypropylmethylcellulose (HPMC) molecular weight, concentration, and food intake conditions on in vivo erosion behavior / Arun Kumar Jain." Greifswald : Universitätsbibliothek Greifswald, 2015. http://d-nb.info/1069195405/34.
Повний текст джерелаSultan, Sadaf [Verfasser], Giuliano [Akademischer Betreuer] Ramadori, Nils [Akademischer Betreuer] Brose, Michael [Akademischer Betreuer] Hoppert, and Uwe [Akademischer Betreuer] Groß. "Serum Lipocalin-2 (LCN-2) as a major acute phase protein under different pathological conditions in vivo and in vitro studies / Sadaf Sultan. Gutachter: Nils Brose ; Michael Hoppert ; Uwe Groß. Betreuer: Giuliano Ramadori." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2012. http://d-nb.info/1042670137/34.
Повний текст джерелаSultan, Sadaf Verfasser], Giuliano [Akademischer Betreuer] Ramadori, Nils [Akademischer Betreuer] Brose, Michael [Akademischer Betreuer] Hoppert, and Uwe [Akademischer Betreuer] [Groß. "Serum Lipocalin-2 (LCN-2) as a major acute phase protein under different pathological conditions in vivo and in vitro studies / Sadaf Sultan. Gutachter: Nils Brose ; Michael Hoppert ; Uwe Groß. Betreuer: Giuliano Ramadori." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2012. http://nbn-resolving.de/urn:nbn:de:gbv:7-webdoc-3400-1.
Повний текст джерелаWright, Victoria Louise. "The role of nicotinic acetylcholine receptors in motivated behaviour." Thesis, University of Bath, 2015. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.687374.
Повний текст джерелаPechloff, Konstanze. "Conditional in vivo expression of the fusion kinase ITK-SYK." Diss., Ludwig-Maximilians-Universität München, 2013. http://nbn-resolving.de/urn:nbn:de:bvb:19-159471.
Повний текст джерелаChaudun, Fabrice. "Involvement of dorsomedial prefrontal projections pathways to the basolateral amygdala and ventrolateral periaqueductal grey matter in conditioned fear expression." Thesis, Bordeaux, 2016. http://www.theses.fr/2016BORD0118/document.
Повний текст джерелаA central endeavour of modern neuroscience is to understand the neural basis of learningand how the selection of dedicated circuits modulates experience-dependent changes inbehaviour. Decades of research allowed a global understanding of the computations occurring inhard-wired networks during associative learning, in particular fear behaviour. However, brainfunctions are not only derived from hard-wired circuits, but also depend on modulation of circuitfunction. It is therefore realistic to consider that brain areas contain multiple potential circuitswhich selection is based on environmental context and internal state. Whereas the role of entirebrain areas such as the amygdala (AMG), the dorsal medial prefrontal cortex (dmPFC) or theperiaqueductal grey matter (PAG) in fear behaviour is reasonably well understood at themolecular and synaptic levels, there is a big gap in our knowledge of how fear behaviour iscontrolled at the level of defined circuits within these brain areas. More particularly, whereas thedmPFC densely project to both the basolateral amygdala (BLA) and PAG, the contributions ofthese two projections pathway during fear behaviour are largely unknown. Beside theinvolvement of these neuronal pathways in the transmission of fear related-information, theneuronal mechanisms involved in the encoding of fear behaviour within these pathways are alsovirtually unknown. In this context, the present thesis work had two main objectives. First,evaluate the contribution of the dmPFC-BLA and dmPFC-vlPAG pathways in the regulation offear behaviour, and second, identify the neuronal mechanisms controlling fear expression in thesecircuits. To achieve these goals, we used a combination of single unit and local field potentialrecordings coupled to optogenetic approaches in behaving animals submitted to a discriminativefear conditioning paradigm. Our results first, identified a novel neuronal mechanism of fear expression based on the development of 4 H oscillations within dmPFC-BLA circuits thatdetermine the dynamics of freezing behaviour and allows the long-range synchronization offiring activities to drive fear behaviour. Secondly, our results identified the precise circuitry at thelevel of the dmPFC and vlPAG that causally regulate fear behaviour. Together these data provideimportant insights into the neuronal circuits and mechanisms of fear behaviour. Ultimately thesefindings will eventually lead to a refinement of actual therapeutic strategies for pathological conditions such as anxiety disorders
Faden, Frederik [Verfasser]. "A simple technique for N-end rule-controlled conditional protein accumulation in vivo / Frederik Faden." Halle, 2017. http://d-nb.info/1138641855/34.
Повний текст джерелаPechloff, Konstanze [Verfasser], and Daniel [Akademischer Betreuer] Krappmann. "Conditional in vivo expression of the fusion kinase ITK-SYK / Konstanze Pechloff. Betreuer: Daniel Krappmann." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2013. http://d-nb.info/1038152178/34.
Повний текст джерелаNakajima, Masaaki. "In Vivo Mechanical Condition Plays an Important Role for Appearance of Cartilage Tissue in ES Cell Transplanted Joint." Kyoto University, 2008. http://hdl.handle.net/2433/124213.
Повний текст джерелаLehnertz, Bernhard. "In vivo characterization of the lysine-methyltransferases Set7/9 and G9a by conditional mutagenesis in the mouse." Thesis, University of British Columbia, 2009. http://hdl.handle.net/2429/13027.
Повний текст джерелаMachiguchi, Toshihiko. "Cellular interactions via conditioned media induce in vivo nephron generation from tubular epithelial cells or mesenchymal stem cells." Kyoto University, 2014. http://hdl.handle.net/2433/189325.
Повний текст джерелаJobin, Christine. "Expansion ex vivo des cellules CD34+ du sang adulte : étude du microenvironnement et caractérisation des cellules générées en condition d'hypoxie." Master's thesis, Université Laval, 2016. http://hdl.handle.net/20.500.11794/27068.
Повний текст джерелаBonneau, Adeline. "Étude de l’impact de la matrice d’un fruit sur la libération et la perception des composés d’arôme en condition in vivo : application à la mangue fraîche ou transformée." Thesis, Montpellier, 2016. http://www.theses.fr/2016MONTT117/document.
Повний текст джерелаRetro-nasal perception of flavor during chewing food in mouth is a complex process. The physico-chemical and aromatic compositions of food, its physical properties, the interactions and reactions involved in oral cavity are the main parameters which can influence flavor perception. In this study, mango is used as model fruit. The study will be focused in better understanding of food disintegration effect, its texture and inter-individual variability on the flavor perception. Liberation of volatile compounds during the chewing of fresh fruit, puree fruit and dried fruit will be studied with RATD (Retronasal aroma trapping device). Fruit volatile profile obtained by SAFE (Solvent assisted flavor evaporation) technique and the aromatic descriptors established during the sensory analysis will be compared with the data from RATD analysis
Hossain, Md Musharraf. "Interactions between Vitamin D, Breast Cancer and Mesenchymal Stem Cells: In Vitro And in Vivo Perspectives." Thesis, The University of Sydney, 2018. http://hdl.handle.net/2123/18682.
Повний текст джерелаMorehead, Melissa L. "Shaping Cows' Approach to Humans Using Positive and Negative Reinforcement." Thesis, University of North Texas, 2005. https://digital.library.unt.edu/ark:/67531/metadc4730/.
Повний текст джерелаAlsheikh, Manal. "Impact of the Maturation Status of Osteoblasts on Their Hematopoietic Regulatory Activity." Thesis, Université d'Ottawa / University of Ottawa, 2017. http://hdl.handle.net/10393/35899.
Повний текст джерелаThomas, R. M. "Csk is an important negative regulator of phagocyte responsiveness in vivo : characterisation of myeloid cell-specific Csk deficiency in mice by conditional mutagenesis (Cre/loxP)." Thesis, University College London (University of London), 2007. http://discovery.ucl.ac.uk/1445173/.
Повний текст джерелаManfredi, I. "In vivo study of mutant nicotinic receptor's role in the pathogenesis of autosomal dominant nocturnal frontal lobe epilepsy : development and characterization of a conditional mouse model." Doctoral thesis, Università degli Studi di Milano, 2008. http://hdl.handle.net/2434/63073.
Повний текст джерелаJokela, T. (Tiina). "Analyses of kidney organogenesis through in vitro and in vivo approaches:generation of conditional Wnt4 mouse models and a method for applying inducible Cre-recombination for kidney organ culture." Doctoral thesis, Oulun yliopisto, 2013. http://urn.fi/urn:isbn:9789526201559.
Повний текст джерелаTiivistelmä Hiirillä geenikohdennuksesta on muodostunut hyödyllinen väline proteiinien tehtävien selvittämisessä ja uusien eläinmallien luomisessa. Cre/loxP -tekniikkaa on käytetty laajasti muuntogeenisten hiirien tuottamisessa. Cre-rekombinaasi tunnistaa spesifisen DNA-jakson, niin kutsutun loxP:n, ja poistaa kaikki DNA-jaksot kahden loxP-sekvenssin väliltä. Cre-rekombinaasin aktiivisuutta voidaan säädellä paikallisesti ja ajallisesti solu- tai kudosspesifisillä promoottoreilla ja synteettisillä indusoivilla kemikaaleilla, kuten tamoksifeenillä tai tetrasykliinillä. Tässä väitöskirjassa hyödynsimme tamoksifeenin aiheuttamaa Cre-rekombinaatiota in vitro -kudosviljelmissä. Cre-ERTM-hiirilinja risteytettiin ROSA26LacZ-reportterilinjan kanssa, ja 4-hydroksitamoksifeenin indusoima Cre-rekombinaasin aktiivisuutta monitoroitiin LacZ–värjäyksellä. 0.5 µM:n 4OH-TM konsentraatiolla LacZ-reportterigeeni saatiin aktivoitua tehokkaasti Cre-rekombinaasin avulla sekä munuaisviljelmissä että munuaismesenkyymiviljelmissä. Wnt4 on erittyvä signalointimolekyyli, jolla on keskeinen rooli useiden elinten, kuten munuaisen, munasarjan, lisämunuaisen, rintarauhasen ja aivolisäkkeen kehittymisessä. Wnt4-geenillä on ratkaisevan tärkeä rooli munuaisen kehityksessä, ja poistogeeninen Wnt4-/-hiiri kuolee pian syntymän jälkeen, todennäköisesti munuaisen vajaatoimintaan. Tässä väitöskirjatyössä tuotettiin kaksi eri Wnt4 alleelia, Wnt4EGFPCre ja konditionaalinen Wnt4. Nämä hiirilinjat analysoitiin, jotta saisimme lisää tietoa Wnt4-geenin toiminnasta ja pystyisimme soveltamaan kyseisiä hiirikantoja munuaisten toiminnan selvittämisessä. Wnt4EGFPCre-alleelissa EGFPCre-fuusio -cDNA kohdennettiin osaksi endogeenisen Wnt4-geenin ykköseksonia. Vihreän fluoresoivan proteiinin (EGFP) aktiivisuus havaittiin varhaisen munuaisen kehityksen aikana. Wnt4EGFPCre-alleelin lisäkarakterisointi reportterilinjoilla (Rosa26LacZ ja Rosa26YFP) osoitti, että Wnt4-geenin ilmentyminen havaittiin munuaisen lisäksi sukurauhasissa, selkäytimessä, keuhkoissa sekä lisämunuaisessa. Wnt4EGFPCre-alleeli ilmentyi niissä kudoksissa, joissa endogeenisen Wnt4-geenin tiedetään olevan aktiivinen. Time-lapse -analyysin avulla osoitettiin, että Wnt4-geeniä ilmentävät solut muodostavat tiettyjä rakenteita munuaisen kehityksen aikana. Wnt4-geeni ilmentyi nefroneissa, kehittyvän virtsajohtimen soluissa sekä useissa medullaarisissa stroomasoluissa. Konditionaalisessa (ehdollisessa) Wnt4 knock-out-hiirilinjassa loxP-sekvenssit sijoitettiin eksonien kolme sekä viisi ympärille. Wnt4-geenin toiminta inaktivoitiin CAGCre- ja Wnt4EGFPCre-hiirilinjojen avulla. Näissä molemmissa tapauksissa Wnt4-geenin toiminnan poistaminen johti munuaisen kehityshäiriöön. Yhteenvetona voimme todeta, että olemme tunnistaneet ne kasvatusolosuhteet, joita voidaan hyödyntää, kun halutaan aktivoida reportterigeenejä tai kehityksen kannalta tärkeitä geenejä tamoksifeenin aiheuttamaa Cre/loxP -rekombinaatiota hyväksikäyttäen kudosviljelmissä. Samoja olosuhteita ja menetelmää käyttäen voidaan myös poistaa jonkun kehityksen kannalta tärkeän geenin toiminta ja tutkia sitä kudosviljelmässä. Tuotetut Wnt4-hiirikannat ovat lisäksi uusia hyödyllisiä työkaluja, kun halutaan tutkia Wnt4-geenin toimintaa erilaisissa kudoksissa ja eri kehitysvaiheiden aikana
Akella, Arun. "A novel in vitro stretch device for simulating in vivo conditions." Thesis, 2018. https://doi.org/10.7912/C2B36V.
Повний текст джерелаBiological cells are constantly subjected to mechanical forces such as tension, compression and shear. The importance of these forces in mediating cell signals, maintenance of lineages, promoting embryonic cell differentiation and tissue engineering is only now coming into focus. It has been shown that stretch stimulus can influence growth, differentiation, as well as tissue strength and integrity. Most stretch systems built to understand more of these phenomena suffer from shortcomings, as accurately replicating the in vivo environment is quite challenging. Many of the devices currently available are very expensive as well as limited to a single application. The objective of this thesis is to design, manufacture, test, and validate a novel uniaxial cyclic cell stretch device that overcomes most of the major limitations of existing systems, and to experimentally demostrate that uniaxial cyclic stretch causes a shift towards in vivo characteristics of smooth muscle cells. The stretch mechanism is driven by a single servo motor which makes its operation simple and straight forward. Coolworks Lite, a proprietary software of the servo motor supplier, is used to control the motor and LabVIEW is used to obtain feedback from the sensors. Validation for the stretch machine was done by evaluating the performance of the device against engineering requirements. Methods were suggested to improve shortcomings that were encountered. Also, the machine's unique design allows its extension to a biaxial stretch unit while keeping the same driver platform, a concept for which has been discussed and illustrated.
Carlson, Kristen Dawn. "Fiber optic confocal microscope: in vivo precancer detection." Thesis, 2006. http://hdl.handle.net/2152/2471.
Повний текст джерелаKursa, Katarzyna. "Effects of external loading conditions on in vivo forces generated by finger flexor muscles /." 2004. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3136091.
Повний текст джерелаLiu, Fei. "Theoretical modeling and experimental validation of in vivo mechanics for subjects having variable cervical spine conditions." 2007. http://etd.utk.edu/2007/LiuFei.pdf.
Повний текст джерела