Дисертації з теми "Viral antigen"
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Clayton, Anne-Louise. "Immunoassays for viral antigen detection in clinical specimens." Thesis, Open University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.254677.
Повний текст джерелаKučinskaitė-, Kodzė Indrė. "Production, Characterization And Application Of New Monoclonal Antibodies Against Viral Antigens." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2011. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2011~D_20110630_104940-67693.
Повний текст джерелаDisertacijoje aprašomi monokloniniai antikūnai, sukurti prieš rekombinantinius mielėse susintetintus antigenus: žmogaus paragripo treciojo tipo viruso, Menangle viruso, hantavirusų bei pasiutligės viruso nukleokapsidės (N) baltymus. Sukurtieji antikūnai buvo visapusiškai charakterizuoti įvairiais imunocheminės analizės metodais, įvertintas jų specifiškumas, afiniškumas, sugebėjimas atpažinti natyvius virusus infekuotų ląstelių kultūrose. Buvo nustatyta, kad antikūnai, sukurti prieš rekombinantinius mielėse susintetintus virusų baltymus, tinka virusų nustatymui infekuotose ląstelėse. Šie tyrimai patvirtino, kad rekombinantiniai mielėse susintetinti virusu N baltymai turi panašias antigenines savybes, kaip natyvūs virusų N baltymai, formuojantys nukleokapsides. Sukurtieji monokloniniai antikūnai taip pat buvo panaudoti išsamiems minėtų virusų N baltymų antigeninės struktūros tyrimams bei imunodominuojančių sekų nustatymui. Disertaciniame darbe gauti duomenys svarbūs, kuriant naujas imunodiagnostikos sistemas, skirtas virusų infekcijoms nustatyti. Disertacija sudaro įvadas, trys skyriai, naudotos literatūros sąrašas ir autorės publikacijų sąrašas. Įvadiniame skyriuje aptariama tiriamoji problema, darbo aktualumas, formuluojamas darbo tikslas bei uždaviniai, darbo mokslinis naujumas ir praktinė reikšmė, pristatomos paskelbtos publikacijos ir pranešimai konferencijose. Pirmasis disertacijos skyrius skirtas literatūros apžvalgai: jame apibūdinamos paragripo virusų, Menangle viruso... [toliau žr. visą tekstą]
Nastke, Maria-Dorothea. "T-cell epitopes from viral and tumor associated antigens induction and analysis of antigen-specific T cells /." [S.l. : s.n.], 2005. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB12168223.
Повний текст джерелаChen, Yun-Chi. "Control of immune response and pathogenesis by antigen during viral infection." Thesis, University of Oxford, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.413974.
Повний текст джерелаGlew, Elizabeth Jane. "The interaction of bovine viral diarrhoea virus with antigen presenting cells." Thesis, University of Reading, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312578.
Повний текст джерелаBiswas, Sumi. "Prime boost vaccination with viral vectors targeting apical membrane antigen 1." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:a17ab4e4-9b81-4ab3-b02f-41d9da36c6ab.
Повний текст джерелаWong, Hiu-ling Beatrice. "Development of antibody and antigen detection assays and vaccines for SARS associated coronavirus." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/hkuto/record/B39634024.
Повний текст джерелаWong, Hiu-ling Beatrice, and 黃曉靈. "Development of antibody and antigen detection assays and vaccines for SARS associated coronavirus." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B39634024.
Повний текст джерелаBhati, Anubhuti. "EXPRESSION OF HEPATITIS C VIRAL NON-STRUCTURAL 3 ANTIGEN IN TRANSGENIC CHLOROPLASTS." Master's thesis, University of Central Florida, 2005. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/3076.
Повний текст джерелаM.S.
Department of Molecular Biology and Microbiology
Burnett College of Biomedical Sciences
Molecular Biology and Microbiology
Lee, Sang-Ryul. "Bovine viral diarrhea virus infections affect professional antigen presentation in bovine monocytes." Diss., Mississippi State : Mississippi State University, 2007. http://library.msstate.edu/etd/show.asp?etd=etd-10222007-092315.
Повний текст джерелаRao, Ankit Rohit. "Engineering an improved dendritic cell vaccine expressing whole antigen following non-viral transfection." Thesis, University of Birmingham, 2012. http://etheses.bham.ac.uk//id/eprint/3622/.
Повний текст джерелаUmami, Rifqiyah Nur. "Effect of Viral and Host Factors on Hepatitis B Virus Surface Antigen Secretion." Thesis, The University of Sydney, 2022. https://hdl.handle.net/2123/29753.
Повний текст джерелаJanbazian, Loury. "Effect of antigen load and viral sequence diversification on HIV-specific CD8+ T cells." Thesis, McGill University, 2010. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=86806.
Повний текст джерелаLes cellules T CD8+ sont impliquées dans le contrôle des infections virales aiguës tels que les virus VIS et VIH. Bien que ces cellules T CD8+ spécifiques au VIH persistent durant la phase chronique de l'infection, leur fonctionnalité semble être altérée par un mécanisme non identifié. Cela a exhorté les chercheurs à revoir les paramètres qui définissent l'efficacité réelle des lymphocytes T CD8+ en générale et surtout lors d'infection par le VIH. À date, il est admis que la polyfonctionnalité des cellules T CD8+ soit l'élément clé de l'efficacité de la réponse immunitaire durant l'infection chronique. De plus, il est établi que la diversité du récepteur de cellule T (RCT) occupe un rôle important et crucial dans le contrôle de la virémie. Bien que plusieurs études sur le répertoire des RCT aient été menés par plusieurs groupes dans le contexte des infections aigues et chronique par le VIH, il n'existe néanmoins pas des données combinant la fluctuation et la fonction du répertoire spécifique au VIH au cours de la progression de la maladie. De ce fait, il était important de réaliser des études longitudinales qui ont pour but principal l'étude de la régénération du répertoire T CD8+ spécifiques au VIH durant les différentes phases de l'infection. Dans mes travaux de doctorat, nous avons exploré les mécanismes responsables des l'inefficacité de cellules CD8+ cours de l'infection par le VIH. En utilisant plusieurs techniques immunologiques et génétiques, nos objectifs visaient à : 1) Définir une signature moléculaire d'épuisement, 2) Étudier l'effet de la charge virale et, 3) Caractériser l'effet de la diversification des séquences virales, sur la clonalité, la fonctionnalité, et le phénotype des cellules T CD8+ spécifiques au VIH. Nous avons en premier lieu identifié le rôle de la molécule PD-1, une des molécules régulatrices qui joue un rôle critique dans le contrôle de la réponse immunitai
Liu, Mengya. "NanoAPC deliver antigen, IL-2 and co-stimulatory molecules to antigen specific T cells and activate viral specific T cells in chronic infections." Thesis, Brunel University, 2011. http://bura.brunel.ac.uk/handle/2438/6413.
Повний текст джерелаWang, Weiping. "SV40 large T antigen helicase roles of the hydrophilic channels and a newly identified unwinding activity /." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 152 p, 2009. http://proquest.umi.com/pqdweb?did=1833646471&sid=7&Fmt=2&clientId=8331&RQT=309&VName=PQD.
Повний текст джерелаPeng, Yu-Cai. "Interactions between polyomavirus large T antigen and the viral replication origin DNA, how and why." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0028/NQ50235.pdf.
Повний текст джерелаPeng, Yu-Cai 1965. "Interactions between polyomavirus large T antigen and the viral replication origin DNA : how and why." Thesis, McGill University, 1999. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=35927.
Повний текст джерелаWhile optimizing the conditions for binding of large T antigen to viral origin DNA, we discovered that binding was substantially stronger at pH 6 to 7 than at pH 7.4 to 7.8, a range often used in DNA binding assays. We showed that increased binding at low pH is due to increased stability of protein-DNA complexes, and that large T antigen molecules self-associated at low pH, forming massive complexes. ATP increased binding of large T antigen to origin DNA by about 2-fold at pH 7.8, but had no detectable effect at pH 7 or below.
Enhanced, stable DNA binding by large T antigen to viral origin DNA at pH 6 enabled us to develop a novel gel mobility shift assay using unfixed protein-DNA complexes. We demonstrated that this assay is very sensitive and highly specific. This method can be used both for detection of large T antigen in crude cell lysates and for quantitation of binding of purified large T antigen to target DNAs under various conditions.
Using a series of point and deletion mutants in the viral origin of DNA replication, we demonstrated that binding of large T antigen to sites 1/2, A, B, and C is cooperative. Binding of large T antigen to one site stimulated binding to other sites 20 to 100 bp distant, and binding to inherently weak sites was strengthened if two or more such sites were present on the same DNA molecule. These findings suggest that large T antigen molecules bound to DNA interact with each other to mutually stabilise their binding.
ATP was shown to stabilise large T antigen-DNA complexes against dissociation only if the DNA contained site 1/2. ATP specifically enhanced protection against DNase I digestion in the central 10 to 12 bp of site 1/2, where hexamers are believed to form and begin unwinding DNA. We propose a model in which large T antigen molecules bound to sites 1/2, A, B, and C on origin DNA form a compact protein-DNA complex via mutual interactions; large T antigen molecules bound to sites A, B, and C are mobilised and "handed over" to site 1/2, where ATP stimulates their assembly into hexamers.
Dubosq, Ming-Celine. "The non-viral production of Chimeric Antigen Receptor T-cells for B-cell haematological malignancies." Thesis, University of Sydney, 2020. https://hdl.handle.net/2123/23170.
Повний текст джерела張紀忠 and Jizhong Zhang. "Conformational antigenic determinants of the HEV CAPSID." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2000. http://hub.hku.hk/bib/B31241360.
Повний текст джерелаZhang, Jizhong. "Conformational antigenic determinants of the HEV CAPSID /." Hong Kong : University of Hong Kong, 2000. http://sunzi.lib.hku.hk/hkuto/record.jsp?B2207918X.
Повний текст джерелаMcMurtrey, Curtis Paul. "Human leukocyte antigen class I presentation and immune recognition of West Nile virus peptide epitopes." Oklahoma City : [s.n.], 2009.
Знайти повний текст джерелаRodríguez, Plata Mª Teresa. "Dendritic Cells and HIV-1: Molecular Mechanisms Involved in Viral Capture, Trans-infection and Antigen Presentation." Doctoral thesis, Universitat Autònoma de Barcelona, 2013. http://hdl.handle.net/10803/120537.
Повний текст джерелаLas células dendrítcas (CD) son las células presentadoras de antígeno más potentes del sistema inmunitario, enlazando la inmunidad innata y la inmunidad adquirida. Sin embargo, las CD pueden contribuir a la diseminación del Virus de la Inmunodeficiencia Humana tipo 1 (VIH-1) mientras intentan inducir una respuesta adaptativa contra la infección viral. El paradigma inmunológico clásico sobre la función de las CD indica que las CD inmaduras principalmente capturan patógenos, mientras que las CD maduras inducen una respuesta adaptativa frente al patógeno capturado. No obstante, la maduración de las CD con lipopolisacárido (LPS) incrementa su abilidad para capturar VIH-1, que, a su vez, resulta en una potente transmisión infecciosa a células diana. El objetivo de esta tesis es el de analizar los mecanismos involucrados en la captura, la trans-infección y la presentación antigénica del VIH-1 por parte de las CD. Con esta finalidad y utilizando técnicas de biología celular y molecular, se ha identificado el ligando viral y el receptor en las CD responsables del mecanismo de captura del VIH-1 independiente de la envuelta viral, el destino del VIH-1 capturado por las CD, y los determinantes para una transmisión infecciosa eficiente de estas partículas virales a los linfocitos T CD4+. Los resultados obtenidos proporcionan nueva información acerca de la contri-bución de las CD en la patogénesis del VIH-1. La maduración de las CD con LPS incrementa la expresión de Siglec-1 en la membrana plasmática de las CD. Este receptor reconoce la sialillactosa de los gangliósido de la membrana del VIH-1, mediando el mecanismo de unión y captura del VIH-1 en las CD independiente de la envuelta viral. Sin embargo, este mecanismo tan eficiente de captura viral en las CD no representa una fuente de antigéno para la presentación antigénica del VIH-1, dado que las partículas virales capturas son retenidas en un compartimento intracelular, protegidas de la degradación y preservando su infectividad. Por consiguiente, en el caso de las CD maduradas con LPS, existe una disociación entre la captura del VIH-1 y su presentación antigénica. Por el contrario, la captura de VIH-1 mediada por Siglec-1 se redirige hacia la transmisión infecciosa a células diana, pero sin que el VIH-1 infecte las CD. Dado que las CD interaccionan continuamente con los célula T CD4+ durante su labor inmunológica, tanto para inducir respuestas inmunes adaptativas como para mantener la homeostasis de las células T, el VIH-1 puede aprovecharse de estos contactos, sin modularlos, para ser transmitido. De esta manera, el VIH-1 pasa desapercibido a través de la sinapsis CD–célula T, escapando del control inmunológico e incrementando la propagación viral, que está marcadamente favorecida por la activación immune inducida por los contactos CD–célula T. Por lo tanto, determinar la contribución de las CD en la patogénesis de la infección por VIH-1 puede ser de gran importancia para el desarrollo de estrategias terapéuticas dirigidas para bloquear la diseminación del VIH-1.
Dendritic cells (DC) are the most potent antigen-presenting cells in the immune system, linking innate and adaptive immune responses. However, it has been suggested a dual role of DC in Human Immunodeficiency Virus type 1 (HIV-1) infection by increasing the spread of HIV-1 while trying to trigger an adaptive response against viral infection. The classical immunological paradigm affirms that immature DC mainly mediate pathogen uptake while mature DC launch adaptive immune responses against the captured pathogen. Nevertheless, DC maturation with lipopolysaccharide (LPS) increases their ability of capturing HIV-1 particles resulting in a potent infectious transmission to target cells. The aim of this thesis is to analyze the mechanisms involved in DC-mediated HIV-1 capture, trans-infection and antigen presentation. To that end, we used methods of cellular and molecular biology, reporting the viral ligand and the DC receptor responsible for an HIV-1 Env-independent uptake mechanism, the fate of the captured HIV-1 particles, and the determinants for an efficient infectious transmission to CD4+ T lymphocytes. Our results provide new insights into contribution of DC to HIV-1 pathogenesis. Maturation of DC with LPS increases the expression of Siglec-1 on DC membrane. This receptor acts as the attachment factor in DC for sialyllactose-containing gangliosides in HIV-1 membrane, mediating the Env-independent mechanism of HIV-1 binding and uptake by DC. However, this efficient mechanism of HIV-1 capture does not represent a source of viral antigen for HLA loading and T-cell activation, given that captured virions are retained within an intracellular compartment, away from degradation, thus preserving their infectivity. Consequently, in the case of HIV-1 and DC matured with LPS, there is dissociation between pathogen uptake and antigen presentation. On the contrary, Siglec-1-mediated HIV-1 capture by DC is redirected to infectious viral transmission to susceptible target cells, without infecting host DC. Since DC continuously interact with CD4+ T lymphocytes during their immunological labor, either to elicit adaptive immune responses or to maintain T-cell homeostasis, HIV-1 can take advantage of these contacts, without modulating them, to be transmitted. As a result, HIV-1 can go unnoticed across the DC–T-cell synapses, bypassing the immunological control and increasing viral dissemination, which is markedly favored by immune activation driven by DC–T-cell contacts. Therefore, determining the contribution of DC to the pathogenesis of HIV-1 infection may be important for the development of therapeutic strategies aiming to block HIV-1 spread.
McLean, Brendan Norbert. "The detection of viral antigen in the cerebrospinal fluid of patients with herpes simplex virus encephalitis." Thesis, University of Edinburgh, 1993. http://hdl.handle.net/1842/26759.
Повний текст джерелаMahauad, Fernandez Wadie Daniel. "Role of bone marrow stromal antigen 2 (BST-2) in viral pathogenesis and breast cancer progression." Diss., University of Iowa, 2016. https://ir.uiowa.edu/etd/6191.
Повний текст джерелаNastke, Maria-Dorothea [Verfasser], and Hans-Georg [Akademischer Betreuer] Rammensee. "T-cell epitopes from viral and tumor associated antigens : induction and analysis of antigen-specific T cells / Maria-Dorothea Nastke ; Betreuer: Hans-Georg Rammensee." Tübingen : Universitätsbibliothek Tübingen, 2005. http://d-nb.info/1162199148/34.
Повний текст джерелаMeei-Yun, Lin. "Evolution of the T Cell Receptor Repertoire during and after Viral Infection: a Dissertation." eScholarship@UMMS, 1999. https://escholarship.umassmed.edu/gsbs_diss/244.
Повний текст джерелаCho, Uhn-Soo. "Structural studies of protein phosphatase 2A, simian virus 40 small t antigen, and the PhoQ sensor domain /." Thesis, Connect to this title online; UW restricted, 2007. http://hdl.handle.net/1773/5677.
Повний текст джерелаWang, Xiaoting Z. "Organ-Dependent and Epitope-Dependent Repertoire Usage and Apoptosis of Antigen-Specific T Cells in Viral Infections: a Dissertation." eScholarship@UMMS, 2004. http://escholarship.umassmed.edu/gsbs_diss/285.
Повний текст джерелаNovak, Erik Joseph. "Tracking antigen-specific immune responses in human infection and disease /." Thesis, Connect to this title online; UW restricted, 2001. http://hdl.handle.net/1773/5084.
Повний текст джерелаZarozinski, Christopher C. "T Cell Receptor-Dependent and Independent Events During Potent Anti-Viral T Cell Responses." eScholarship@UMMS, 1998. http://escholarship.umassmed.edu/gsbs_diss/175.
Повний текст джерелаCarlsson, Björn. "Adoptive T Cell Therapy of Viral Infection and Cancer : Ex vivo Expansion of Cytomegalovirus- and Prostate Antigen-specific T Cells." Doctoral thesis, Uppsala University, Clinical Immunology, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4821.
Повний текст джерелаThe main focus of my thesis has been to develop protocols for generating antigen-specific cytotoxic T lymphocytes (CTLs) and T helper cells (TH) for adoptive transfer to treat cytomegalovirus (CMV) disease and prostate cancer. CMV viremia is a severe complication in immunocompromised stem cell transplanted patients. Prostate cancer is a leading cause of death for men in Western countries. Although different in nature, CMV-infected cells and prostate cancer cells can both be eliminated through specific activation of the adaptive immune system.
To generate CMV pp65-specific T cells, I utilized dendritic cells (DCs) modified with an HLA-A*0201/pp65495-503 peptide, a recombinant adenovirus coding for pp65, in vitro transcribed pp65 mRNA and a recombinant pp65 protein. Peptide stimulation yielded large numbers of peptide-specific CD8+ T cells with high lytic activity while adenovirus or mRNA stimulation resulted in the expansion of CTLs against multiple pp65 epitopes. The recombinant protein activated primarily CD4+ TH cells. Stimulation with DCs co-modified with pp65 mRNA and pp65 protein simultaneously generated both pp65-specific CTLs and TH cells. Such T cells would cover all pp65 epitopes while avoiding potential virus related biohazards. The mRNA/protein combinatory approach can be used to stimulate T cells ex vivo from virtually all stem cell donors for adoptive T cell transfer.
I have identified two immunogenic HLA-A*0201-restricted peptide epitopes from the prostate tissue antigen TARP. Repeated stimulations with TARP peptide-pulsed DCs yielded up to 20% TARP-directed CD8+ T cells even when starting from undetectable frequencies (<0.01%). The T cells could be sorted to 99% purity and expanded 1000-fold with retained specificity and activity. We also detected TARP-directed CD8+ T cells in the blood of prostate cancer patients. Therefore, TARP seems to have potential as antigen in DC vaccination or adoptive T cell therapy of prostate cancer.
Abeywickrama, Samarakoon Natali. "Small hepatitis Delta antigen mimics a histone H3 epitope to facilitate the remodeling of the Hepatitis D virus (HDV) viral ribonucleoprotein." Thesis, Lyon, 2016. http://www.theses.fr/2016LYSE1193.
Повний текст джерелаHepatitis Delta Virus (HDV) is a satellite of Hepatitis B Virus (HBV), leading to more severe life threatening liver diseases than HBV mono–infection. No efficient therapy is available against HDV and the estimated 15 million HDV infected individuals worldwide are at a high risk of cirrhosis and hepatocellular carcinoma. HDV is a unique RNA virus as it does not encode a viral polymerase. HDV RNA replication occurs via a double rolling circle mechanism generating unit–length genomic or antigenomic RNA strands. The synthesis of the genomic RNA is sensitive to low concentrations of α–amanitin, suggesting that the RNA–dependent RNA synthesis is mediated by DNA–dependent RNA polymerase II (RNA Pol II). This process relies on the HDV encoded Small Hepatitis Delta antigen (S–HDAg), which must be acetylated at K72 to activate the synthesis of the genomic RNA. We recently identified BAZ2B (Bromodomain Associated to Zinc finger protein 2B) as a major interactant of S–HDAg by affinity capture coupled to mass spectrometry in differentiated HepaRG cells. The biological function of BAZ2B is however unknown. In comparison with related BAZ proteins (BAZ–1A/1B/2A), it is postulated that BAZ2B is the accessory subunit of a new chromatin remodeling complex of ISWI–type, which regulates nucleosome positioning through ATP hydrolysis. Recent studies revealed that the BAZ2B bromodomain (BRD) recognizes the distinct epigenetic signature K14ac–X–X–R on histone H3. This suggests that the mode of action of BAZ2B associated chromatin remodeling complex involves recognizing propagated specific histone acetylation marks to subsequently alter the chromatin dynamic and recruit the RNA Pol II for transcriptional activation. We hypothesized that the p300–mediated acetylation of the conserved K72–X–X–R motif in S–HDAg mimics acetylated histones on the pseudo–double stranded antigenomic RNA, to recruit the BAZ2B associated chromatin remodeling complex to initiate RNA Pol II mediated synthesis of HDV genome. To confirm the functional relevance of BAZ2B recruitment for HDV replication, we transfected Huh 7 cells stably expressing either wild–type S–HDAg or R75A mutant S–HDAg with the HDV replication defective plasmid pSVLD2m. Our results indicate that the synthesis of genomic RNA was greatly reduced in cells expressing the R75A mutant S–HDAg in comparison to cells expressing wild–type S–HDAg, whereas the amount of antigenomic RNA remained the same in both cases. Co–crystallization experiments are currently being carried out to better characterize at the molecular level the association between BAZ2B BRD and S–HDAg derived peptides. Furthermore, siRNA experiments directed against the BAZ2B gene are expected to reveal the consequences of BAZ2B inhibition on HDV viral replication. The involvement of BAZ2B in HDV replication may open anti–HDV drug development opportunities, based on the optimization of emerging BAZ2B–BRD inhibitors
Moetlhoa, Boitumelo. "The preparation of antigen for the generation of polyclonal antibodies against the capsid subunit, VP1, and the viral protease, 3Cpro, of Theiler's murine encephalomyelitis virus (TMEV)." Thesis, Rhodes University, 2014. http://hdl.handle.net/10962/d1013225.
Повний текст джерелаCatamo, Eulalia. "Genetic variation in hla-g: its influence in autoinflammatory autoimmune and viral diseases." Doctoral thesis, Università degli studi di Trieste, 2014. http://hdl.handle.net/10077/9982.
Повний текст джерелаL'antigene leucocitario umano (HLA)-G presenta, in condizioni fisiologiche, una ristretta espressione tessuto-specifica ed ha funzione immuno-tollerogenica. Però, la presenza della molecola HLA-G è stata associata a diverse patologie autoimmuni e virali. In questo progetto di dottorato di ricerca abbiamo analizzato la possibile associazione tra la varianti genetiche nel gene HLA-G, che si suppone regolino l'espressione di HLA-G, e la suscettibilità allo sviluppo e al decorso della malattia celiaca, del lupus eritematoso sistemico, dell'artrite reumatoide e dell'infezione dal virus dell'epatite C. Inoltre, abbiamo analizzato se variazioni all'interno del promotore di HLA-G possano alterare la sua trascrizione genica, a questo scopo è stato condotto il saggio della luciferasi. Per gli studi di associazione sono stati analizzati 800 bp del promotore, l’intero 3’UTR e la delezione di una citosina all’esone 3 (ΔC, allele HLA-G*0105N) in 402 pazienti celiaci e 509 controlli italiani; 114 pazienti con lupus eritematoso sistemico e 128 controlli sani provenienti dal Nord -Est del Brasile, 127 pazienti con artrite reumatoide e 128 controlli dal Nord-Est del Brasile, e 286 pazienti caucasici HCV positivi e 285 controlli provenienti dalla stessa area geografica. Il saggio della luciferasi è stato condotto su 9 diversi aplotipi al promotore del gene HLA-G: CCTAGGACCG, CGTAGGACCG, CTTAGGACCG, TCGGTACGAA, TGGGTACGAA, TTGGTACGAA, CCTAGGAGCG, CGTAGGAGCG, e CTTAGGAGCG. Numerosi SNPs e aplotipi del gene HLA-G sono stati associati con le malattie analizzate. Inoltre, il saggio della luciferasi ha permesso di constatare che la presenza di polimorfismi, nel promotore del gene HLA-G, altera la trascrizione genica; nello specifico, in condizioni di stress, l’allele -725 C era significativamente associato ad un aumento della trascrizione del gene rispetto agli alleli -725 G e T. I nostri i risultati indicano un'associazione tra i polimorfismi del gene HLA-G e la suscettibilità allo sviluppo delle malattie studiate, suggerendo che molecola HLA-G è coinvolta nella patogenesi di queste malattie. Inoltre, possiamo ipotizzare che il gene HLA-G sia un gene stress-inducibile e che la presenza di SNPs al promotore alterati i livelli di trascrizione del gene
The Human Leukocyte Antigen (HLA)-G present a physiological restricted tissue-specific expression and an immuno-tolerogenic functions. The HLA-G expression has been associated with various autoimmune and viral diseases. In this PhD project we analyzed the possible association between genetic variant in the HLA-G gene, supposed to regulate HLA-G expression, and the susceptibility to develop celiac disease, systemic lupus erythematosus, rheumatoid arthritis and hepatitis C virus infection. Furthermore, we analyzed if variations within the HLA-G promoter could alter its transcription, and for this reason luciferase reporter gene assays was conducted. The HLA-G 5’ upstream regulatory region (URR), 3’ untranslated region (UTR) and a cytosine deletion at exon 3 (ΔC, HLA-G*0105N allele) were analyzed in 402 celiac patients and 509 controls from Italy; 114 systemic lupus erythematosus patients and 128 healthy controls from North East Brazil; 127 rheumatoid arthritis patients and 128 controls from North East Brazil; and 286 Hepatitis C virus Caucasian patients and 285 controls from the same geographical area. The luciferase reporter gene assay was used for the HLA-G promoter CCTAGGACCG, CGTAGGACCG, CTTAGGACCG, TCGGTACGAA, TGGGTACGAA, TTGGTACGAA, CCTAGGAGCG, CGTAGGAGCG, and CTTAGGAGCG haplotypes. Several HLA-G SNPs and haplotypes were associated with the diseases analyzed. By luciferase reporter gene assay, we found that the presence of polymorphisms in the HLA-G promoter altered gene transcription, specifically -725 C allele was significantly associated with an increased of the HLA-G transcription with respect to -725 G and T alleles in stress condition. Our findings indicate an association between HLA-G gene polymorphisms and susceptibility to diseases development, suggesting that HLA-G molecule is involved in the pathogenesis of the diseases. Also, we can hypothesize that HLA-G gene is a stress-inducible gene and that the presence of SNPs to the promoter alter levels of transcription of the gene
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Almqvist, Jenny. "Epstein-Barr virus nuclear antigen 1, Oct & Groucho/TLE in control of promoter regulation /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-523-2/.
Повний текст джерелаWarnatsch, Annika. "Impact of proteasomal immune adaptation on the early immune response to viral infection." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2013. http://dx.doi.org/10.18452/16775.
Повний текст джерелаAn efficient immune control of virus infection is predominantly mediated by CD8+ T cells which patrol through the body and eliminate infected cells. Infected cells are recognized when they present viral antigenic peptides on their surface via MHC class I molecules. To make antigenic peptides available for loading on MHC class I complexes, the ubiquitin proteasome system plays a crucial role. Moreover, the induction of the i-proteasome is known to support the generation of MHC class I ligands. Recently, new functions of the i-proteasome have been discovered. Evidence is increasing that the i-proteasome is involved in the protection of cells against oxidative stress. Within this thesis the characteristic of the i-proteasome to protect cells against the accumulation of oxidant-damaged proteins could be linked to its role in improving the generation of MHC class I ligands. It could be demonstrated that during a virus infection in non-immune cells the production of reactive oxygen species by the alternative NADPH oxidase Nox4 is of critical importance resulting in the accumulation of potentially toxic oxidant-damaged proteins. Indeed, within two hours of infection structural virus proteins were oxidized and subsequently poly-ubiquitylated. The concomitant formation of i-proteasomes led to a rapid and efficient degradation of ubiquitylated virus antigens thereby improving the liberation of immunodominant viral epitopes. In conclusion, a so far unknown mechanism to fuel proteasomal substrates into the MHC class I antigen presentation pathway has been revealed. A new protein pool consisting of exogenously delivered viral proteins provides proteasomal substrates in the very early phase of a virus infection. Within the scope of this thesis the i-proteasome has been shown to link the protection against oxidative stress, initiated directly by pathogen recognition, with the generation of antigenic peptides. Together, an effective adaptive immune response is triggered.
Söderholm, Jonas. "The hepatitis C virus and immune escape : relation between sequence variations and the in vitro and in vivo functionality of the non-structural 3/4A complex /." Stockholm, 2006. http://diss.kib.ki.se/2006/91-7357-042-7/.
Повний текст джерелаTrujillo, Jonathan Anthony. "T cell responses to S-glutathionylated And heteroclitic viral epitopes and CCl2-mediated immune dysregulation in mice infected with a neurotropic coronavirus." Diss., University of Iowa, 2014. https://ir.uiowa.edu/etd/4775.
Повний текст джерелаMonjezi, Razieh [Verfasser], and Michael [Gutachter] Hudecek. "Engineering of chimeric antigen receptor T cells with enhanced therapeutic index in cancer immunotherapy using non-viral gene transfer and genome editing / Razieh Monjezi ; Gutachter: Michael Hudecek." Würzburg : Universität Würzburg, 2018. http://d-nb.info/1162062231/34.
Повний текст джерелаLazaro, Estibaliz. "Impact du sous-type viral et de l’apprêtement antigénique sur la présentation des épitopes du VIH-1 par les molécules HLA de classe I." Thesis, Bordeaux 2, 2010. http://www.theses.fr/2010BOR21776/document.
Повний текст джерелаHIV-specific cytotoxic T lymphocytes (CTLs) play a critical role for clearance of virus-infected cells and induction of these cells is a necessary component of any successful vaccine strategy against AIDS. Therefore, identification of the factors defining and modulating the efficiency of these protective responses are urgently needed. We focused our study on two factors potentially involved in HLA recognition: HIV-1 sub-type and antigen processing.The extreme variability of the virus with to date 11 HIV-1 subtypes and 48 circulant recombinant forms (CRFs) circulating worlwide among heterogeneous populations imply high polymorphism and different mutational escape patterns.We demonstrate that among the HIV-1 infected Vietnamese population where the CRF01_AE is largely predominant, the HLA binding of known CTL epitopes is strongly reduced compared to the subtype B due to intraepitopic mutations, facilitating immune evasion of these viral strains.Moreover, we show that the presentation of adequate amounts of epitopes leading to CTL recognition depends on the subset cells involved in the antigen processing, monocytes having a significantly higher and more efficient proteolytic activity. Using in vitro degradation assays, we measured the intracellular HIV-1 epitope stability and demonstrated that this factor is highly variable, sequence dependent and also contributes to a more efficient presentation.Together, these data indicate that, besides HLA and TCR binding and exhaustion factors, HIV-1 CTL recognition can also be modulated by the viral sub-type and the antigen processing machinery
Chernyukh, O. G. "Qualitative detection of Ig G antibodies to SARS-CoV-2 coronavirus nucleocapsid antigen in the blood serum of patients who had viral respiratory infection, caused by SARS-CoV-2." Thesis, БДМУ, 2022. http://dspace.bsmu.edu.ua:8080/xmlui/handle/123456789/19534.
Повний текст джерелаXu, Dan. "Cellular Immunity in Recombinant Adeno-Associated Virus Vector Mediated Gene Therapy." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1313504203.
Повний текст джерелаMukherjee, Siddhartha. "The processing and presentation of viral antigens." Thesis, University of Oxford, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.390538.
Повний текст джерелаMcMahon, Christopher Wolcott. "Structure-function analysis of mouse mammary tumor virus superantigens /." Thesis, Connect to this title online; UW restricted, 1997. http://hdl.handle.net/1773/8342.
Повний текст джерелаLafon, Monique. "La nucleocapside du virus rabique : une nouvelle cible pour la reponse immunitaire et pour la therapie." Paris 7, 1987. http://www.theses.fr/1987PA077219.
Повний текст джерелаJowett, Jeremy Bryan Mark. "Properties of recombinant HIV and SIV antigens." Thesis, University of Oxford, 1992. http://ora.ox.ac.uk/objects/uuid:5de3119b-4aa1-469a-80b6-f1f28dff4f5c.
Повний текст джерелаPerry, Sara Jane St John. "Novel delivery systems for SIV antigens." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321085.
Повний текст джерелаStevenson, Philip G. "The immune response to viral antigens in the mouse brain." Thesis, University of Oxford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364023.
Повний текст джерелаMAZZOLENI, ELISA. "Exploration of new techniques for purification and chemo-selective conjugation of bioreagents for immunodiagnostic applications." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2015. http://hdl.handle.net/10281/68468.
Повний текст джерелаAntigen and antibody are the two key reagents for an immunodiagnostic assay. Investigation of new techniques and improvement of processes such as purification and site-specific labeling of antigen and antibody molecules can promote the development of new more powerful bioreagents able of improving the performance of immunodiagnostic assays. The first part of this thesis aimed to explore innovative biotechnology techniques in antigen production for the improvement of immunoassays that allow the detection of antibodies directed against the Epstein-Barr virus. EBV virus is the causative agent of infectious mononucleosis and it is considered to be associated with a still increasing number of tumors; for this reason it is important to develop diagnostic assays for EBV detection with high specificity and sensitivity. The viral capsid protein VCA p18 is one of the most important antigens for the diagnosis of EBV. The current Diasorin LIAISON EBV VCA IgM and IgG assays rely on a single antigen, consisting in a synthetic peptide corresponding to the immunodominant C-terminal portion of the p18 protein, which is immobilized on solid phase (indirect format). The several methods explored in this thesis have allowed to obtain different variants of the p18 antigen with the aim to improve the performance of DiaSorin LIAISON EBV VCA IgM and IgG assays at different levels: 1_production of p18 antigen; 2_immobilization of p18 antigen on solid phase; 3_immunoassay format. 1_ The length of the immunodominant C-terminal portion of the p18 protein (57aa) appears to be considerable for the synthetic route but, at the same time, too small to be effectively produced in a recombinant fashion. To overcome this problem, we explored the Elastin Like Polypeptides (ELP)-Intein system, a method based on the use of a self-cleavable protein (the intein) and a temperature responsive tag (ELP). This technique has proved to be an excellent system for the preparation of the p18 peptide. 2_The development of different variants of p18 antigen has enabled to explore different techniques for the immobilization of the same antigen on solid phase: direct covalent coating, through streptavidin-biotin complex and through an innovative procedure based on the use of leucine zipper (or “velcro”) peptides. The immobilization of the p18 peptide on solid phase through these different methods occurred successfully and the immunochemical activity of the antigen, immobilized with these innovative techniques, is comparable or better than that of the synthetic peptide used in the current immunoassays. 3_Despite the DiaSorin LIAISON EBV VCA IgM immonoassay has a good analytical performance, in order to obtain an increase of specificity, a new assay format was explored. Unfortunately, the results indicate that this different type of format reaches a lower level of specificity than that of current assay. The second part of this thesis aimed to explore an innovative method for the site-specific labeling of antibodies. One the most promising approach is based on the generation of free thiol groups by selective partial reduction of the interchain disulfide bridges present at the level of the “hinge region” and their reaction to labels carrying sulfhydryl-reactive chemical groups. This technology was used for the biotinylation of two different antibodies currently used in immunoassays for the HIV p24 viral protein and for FGF23 antigen detection. The results suggest that the site-specific biotinylation compared to the random traditional biotinylation promotes a great improvement of antibodies immunochemical activity with a consequent optimization of immunodiagnostic assays performance.
Gall, Jason G. D. "The role of adenovirus fiber and hexon proteins in virus-host interactions /." Access full-text from WCMC:, 1998. http://proquest.umi.com/pqdweb?did=1432807591&sid=9&Fmt=2&clientId=8424&RQT=309&VName=PQD.
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