Дисертації з теми "Vertebrate DNA"
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Elango, Navin. "Evolutionary impacts of DNA methylation on vertebrate genomes." Diss., Atlanta, Ga. : Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/26691.
Повний текст джерелаCommittee Chair: Dr. Soojin Yi; Committee Member: Dr. Eric Vigoda; Committee Member: Dr. James Thomas; Committee Member: Dr. John McDonald; Committee Member: Dr. Kirill Lobachev; Committee Member: Dr. Michael Goodisman. Part of the SMARTech Electronic Thesis and Dissertation Collection.
Schofield, Julian Paul. "Studies on the structure of vertebrate genes." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386858.
Повний текст джерелаLong, Hannah Katherine. "Evolutionary usage and developmental roles of vertebrate non-methylated DNA." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:78b14c1d-1fa3-46f1-815f-a8ba55579c43.
Повний текст джерелаChua, Shijia Joy. "Establishing the role of RNF4 in the vertebrate DNA damage response." Thesis, University of Dundee, 2012. https://discovery.dundee.ac.uk/en/studentTheses/20679582-0300-40ac-8d30-e733210c76b4.
Повний текст джерелаBarr, Alexis. "Characterising the function of CDK5RAP2 in the vertebrate centrosome." Thesis, University of Cambridge, 2010. https://www.repository.cam.ac.uk/handle/1810/228639.
Повний текст джерелаVeeman, Michael Terrence. "Zebrafish prickle : non-canonical Wnt/PCP functions in vertebrate gastrulation /." Thesis, Connect to this title online; UW restricted, 2003. http://hdl.handle.net/1773/4999.
Повний текст джерелаGohm, Birgit [Verfasser]. "The Role of DNA Repair in the Evolution of Vertebrate Longevity / Birgit Gohm." Konstanz : Bibliothek der Universität Konstanz, 2016. http://d-nb.info/1171131860/34.
Повний текст джерелаYoshimura, Michio. "Vertebrate POLQ and POLβ cooperate in base excision repair of oxidative DNA damage". Kyoto University, 2007. http://hdl.handle.net/2433/135652.
Повний текст джерелаParker, Hugo. "The role of highly conserved non-coding DNA sequences in vertebrate development and evolution." Thesis, Queen Mary, University of London, 2010. http://qmro.qmul.ac.uk/xmlui/handle/123456789/1267.
Повний текст джерелаBednar, Theresa [Verfasser], George [Akademischer Betreuer] Iliakis, and Jürgen [Akademischer Betreuer] Thomale. "Efficient support of DNA replication functions by DNA ligase 3 in vertebrate cells / Theresa Bednar. Gutachter: Jürgen Thomale. Betreuer: George Iliakis." Duisburg, 2013. http://d-nb.info/1042373493/34.
Повний текст джерелаRoach, Jared C. "Random subcloning, pairwise end sequencing, and the molecular evolution of the vertebrate trypsinogens /." Thesis, Connect to this title online; UW restricted, 1998. http://hdl.handle.net/1773/8331.
Повний текст джерелаBianchi, Julie. "Investigating the role of a novel primase-polymerase, PrimPol, in DNA damage tolerance in vertebrate cells." Thesis, University of Sussex, 2013. http://sro.sussex.ac.uk/id/eprint/45544/.
Повний текст джерелаNakamura, Kyoko. "Genetic dissection of vertebrate 53BP1 : a major role in non-homologous end joining of DNA double strand breaks." Kyoto University, 2008. http://hdl.handle.net/2433/135861.
Повний текст джерелаKohzaki, Masaoki. "Cooperative roles of vertebrate Fbh1 and Blm DNA helicases in avoidance of crossovers during recombination initiated by replication fork collapse." Kyoto University, 2007. http://hdl.handle.net/2433/135695.
Повний текст джерелаSangrithi, Mahesh Nataraj. "Insights into the functions of a novel vertebrate RecQ helicase in chromosomal DNA replication and in the response to stalled replication forks." Thesis, University of Cambridge, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.616261.
Повний текст джерелаWhiteman, Erin Tuxford. "The development of a real-time PCR multiplex TaqMan™ assay for vertebrate class in non-human DNA samples for forensic science." Thesis, Whiteman, Erin Tuxford (2021) The development of a real-time PCR multiplex TaqMan™ assay for vertebrate class in non-human DNA samples for forensic science. Masters by Research thesis, Murdoch University, 2021. https://researchrepository.murdoch.edu.au/id/eprint/64836/.
Повний текст джерелаSu, Xinyi. "A vertebrate N-end rule conditional protein degradation system and its use in temporal dissection of DNA transactions in a single cell cycle." Thesis, University of Cambridge, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.612541.
Повний текст джерелаKhare, Dheeraj. "Crystallographic and functional study on DNA binding proteins: repressor and partitioning protein KorB from RP4 plasmid and the transposase "Sleeping Beauty" of vertebrate origin." [S.l. : s.n.], 2004. http://www.diss.fu-berlin.de/2004/155/index.html.
Повний текст джерелаKatragadda, Chakravarthy. "Survey of Ehrlichia and Anaplasma species in white tailed deer and in ticks by real-time RT-PCR/PCR and DNA sequencing analysis." Thesis, Kansas State University, 2011. http://hdl.handle.net/2097/9158.
Повний текст джерелаDepartment of Diagnostic Medicine/Pathobiology
Roman Reddy R. Ganta
Ehrlichia and Anaplasma species are rickettsial organisms which infect a variety of mammalian species. The organisms are transmitted from ticks and are maintained in reservoir hosts. Several pathogens have been identified in recent years as the causative agents for emerging infections in people. One of the primary reservoir hosts for the pathogens is the white tailed deer. In this study, 147 deer blood samples and 37 ticks were evaluated for the prevalence of Ehrlichia/Anaplasma species by TaqMan-based real time amplification assay and DNA sequence analysis. One hundred and thirteen (74%) samples tested positive with the Ehrlichia/Anaplasma genera-specific probe. Further analysis of the samples with the probes specific for human ehrlichiosis agents, E. chaffeensis and E. ewingii identified 4 (2.7%) and 7 (4.7%) positives, respectively. Test positives from 24 randomly selected samples were further evaluated by sequence analysis targeting to a 450 bp segment of 16S rRNA gene. All 24 samples were confirmed as positive for the Ehrlichia GA isolate # 4 (GenBank #U27104.1). DNAs from 37 pools of ticks collected from the white tailed deer were also evaluated. The TaqMan-based real time PCR assay with Anaplasma/Ehrlichia common probe identified 29 (78%) tick pools as positives whereas E. chaffeensis- and E. ewingii-specific probes identified three (8%) and one (3%) positives, respectively. The PCR and sequence analysis of tick samples identified Gram-negative bacteria species which included one endosymbiont of Rickettsia species (one tick pool), one Alcaligenes faecalis strain (three tick pools), five different Pseudomonas species (9 tick pools) and five different uncultured bacteria organisms (7 tick pools). Although the pathogenic potential of the white-tailed deer isolates of Anaplasma and Ehrlichia agents remains to be established, their high prevalence and the presence of human ehrlichiosis pathogens in white-tailed deer is similar to earlier findings. The high prevalence of the deer isolates of Anaplasma and Ehrlichia species demonstrates the need for further assessment of the pathogenic potential of these organisms to people and domestic animals.
Ryan, James L. "Multiscale landscape genetics analysis of feral pigs in the Herbert region of far-north Queensland." Thesis, Queensland University of Technology, 2022. https://eprints.qut.edu.au/227325/1/James_Ryan_Thesis.pdf.
Повний текст джерелаMeng, Anming. "DNA fingerprinting and minisatellite variation of swans." Thesis, University of Nottingham, 1990. http://eprints.nottingham.ac.uk/13889/.
Повний текст джерелаLau, Tze Chin. "In vitro and in vivo analyses of the impact of Mab21l2 and its targets on neural patterning and differentiation in vertebrates /." View abstract or full-text, 2010. http://library.ust.hk/cgi/db/thesis.pl?BIOL%202010%20LAU.
Повний текст джерелаPetruti-Mot, Anca. "Genetic and functional analysis of topoisomerase II in vertebrates." Thesis, University of Edinburgh, 2000. http://hdl.handle.net/1842/8985.
Повний текст джерелаYant, Stephen Russell. "Sleeping beauty : a DNA transposon system for therapeutic gene transfer in vertebrates /." Thesis, Connect to this title online; UW restricted, 2001. http://hdl.handle.net/1773/5068.
Повний текст джерелаHaynes, Susan. "The history of wild and domesticated vertebrates deduced from modern and ancient DNA sequences." Thesis, University of York, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.325669.
Повний текст джерелаCordonnier, Agnès. "Etude des mecanismes moleculaires de la replication de l'adn mitochondrial de xenopus laevis." Paris 6, 1987. http://www.theses.fr/1987PA066156.
Повний текст джерелаMilinkovitch, Michel. "Phylogenetic analyses of molecular data in vertebrates with special emphasis on the implications of mitochondrial DNA sequences for reevaluating morphological and behavioral evolution in cetaceans." Doctoral thesis, Universite Libre de Bruxelles, 1994. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/212689.
Повний текст джерелаOrfanoudakis, Georges. "Diadenosine tetraphosphate : implication dans l'activite mitotique, la replication et la reparation du dna." Université Louis Pasteur (Strasbourg) (1971-2008), 1988. http://www.theses.fr/1988STR13121.
Повний текст джерелаTratner, Isabelle. "Etude de la methylation des genes codant pour l'albumine et l'alphafoetoproteine dans differents tissus au cours du developpement et dans des cellules d'hepatomes chez le rat : correlation avec l'expression genique." Paris 6, 1987. http://www.theses.fr/1987PA066651.
Повний текст джерелаBonne-Andrea, Catherine. "Contribution à l'étude des propriétés et du rôle biologique de la protéine non-histone HMG1." Paris 6, 1986. http://www.theses.fr/1986PA066451.
Повний текст джерелаDunon-Bluteau, Dominique. "Etude de la region origine de replication de l'adn mitochondrial de xenopus laevis." Paris 7, 1987. http://www.theses.fr/1987PA077049.
Повний текст джерелаWion, Didier. "Etude de la regulation du nerve growth factor (ngf) : clonage du gene ngf de poulet et d'un cdna codant pour la chaine lourde des neurofilaments." Paris 7, 1988. http://www.theses.fr/1988PA077168.
Повний текст джерелаBlanchetot, Alain. "Organisation, structure et comparaison du gène de la myoglobine (mb) chez les mammifères." Paris 6, 1986. http://www.theses.fr/1986PA066602.
Повний текст джерелаGILSON, GEORGES. "Le diadenosine tetraphosphate : etudes immunochimiques et analyse de son role dans les mecanismes de reponse au stress et de reparation du dna." Université Louis Pasteur (Strasbourg) (1971-2008), 1988. http://www.theses.fr/1988STR13110.
Повний текст джерелаSari, Marie-Agnès. "Syntheses de porphyrines cationiques solubles dans l'eau et etude de leurs interactions avec l'adn de thymus de veau." Paris 6, 1988. http://www.theses.fr/1988PA066528.
Повний текст джерелаSmedley, Marie A. "Nutritional and environmental effects on triploid Atlantic salmon skeletal deformity, growth and smoltification." Thesis, University of Stirling, 2016. http://hdl.handle.net/1893/23342.
Повний текст джерелаMoris, Gilbert. "Le diadenosine tetraphosphate : roles dans la regulation de la croissance et du cycle cellulaires et dans la reponse cellulaire a un stress a l'ethanol." Université Louis Pasteur (Strasbourg) (1971-2008), 1988. http://www.theses.fr/1988STR13118.
Повний текст джерелаFarache, Gabriel. "Etudes des regions genomiques delimitant les domaines des genes globine chez le poulet." Paris 7, 1987. http://www.theses.fr/1987PA077009.
Повний текст джерелаKaushik, Azad Kumar. "Etude idiotypique, structurale et analyse du repertoire des autoanticorps dans deux modeles d'autoimmunite naturelle chez la souris : autoanticorps reagissant avec les globules rouges de souris bromelises, autoanticorps reagissant avec l'adn et les." Paris 7, 1987. http://www.theses.fr/1987PA077074.
Повний текст джерелаHEILIG, ROLAND. "Identification du gene de la myopathie de duchenne : caracterisation du messager chez le poulet : etude de la dystrophine." Université Louis Pasteur (Strasbourg) (1971-2008), 1989. http://www.theses.fr/1989STR13011.
Повний текст джерелаWalter, Philippe. "Le recepteur de l'oestradiol humain : isolement, sequence et expression de l'adnc et homologie avec l'oncogene erb-a." Université Louis Pasteur (Strasbourg) (1971-2008), 1986. http://www.theses.fr/1986STR13003.
Повний текст джерелаSobczak, Joëlle. "Etude de l'expression genetique dans le foie de rat en hypertrophie compensatrice." Paris 6, 1988. http://www.theses.fr/1988PA066543.
Повний текст джерелаLabidi, Brahim. "Etude de la transcription dans des noyaux contenant un seul chromosome." Grenoble 2 : ANRT, 1987. http://catalogue.bnf.fr/ark:/12148/cb37606769q.
Повний текст джерелаRonquist, Göran. "Some Characteristics of Human Prostasomes and Their Relationship to Prostate Cancer." Doctoral thesis, Uppsala universitet, Klinisk kemi, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-100799.
Повний текст джерелаGiraudat, Jérôme. "Etude de l'organisation fonctionnelle des chaines polypeptidiques du récepteur nicotinique de l'acétylcholine." Paris 6, 1986. http://www.theses.fr/1986PA066403.
Повний текст джерелаLegrand, Alain. "Liposomes cibles et vecteurs retroviraux pour le transfert et l'expression du gene de la preproinsuline i de rat dans des cellules eucaryotes." Orléans, 1987. http://www.theses.fr/1987ORLE2011.
Повний текст джерелаDelmas, Véronique. "Structure et proprietes biologiques du papovavirus de hamster." Paris 6, 1986. http://www.theses.fr/1986PA066550.
Повний текст джерела"Molecular cloning of vertebrate growth hormone receptor complementary DNAs." 1996. http://library.cuhk.edu.hk/record=b5895740.
Повний текст джерелаYear shown on spine: 1997.
Thesis (M.Phil.)--Chinese University of Hong Kong, 1996.
Includes bibliographical references (leaves 141-149).
Acknowledgments --- p.i
List of Contents --- p.ii
List of Figures --- p.viii
List of Tables --- p.xii
List of Primers --- p.xiii
Abbreviations --- p.xiv
Chapter Chapter 1 --- Introduction
Chapter 1.1 --- Growth Hormone (GH) --- p.1
Chapter 1.2 --- Growth Hormone Receptor (GHR) --- p.3
Chapter 1.2.1 --- Tissue Distribution of GHR --- p.4
Chapter 1.2.2 --- Biosynthesis and Degradation of GHR --- p.6
Chapter 1.2.3 --- Regulation of GHR Level --- p.7
Chapter 1.2.4 --- The Structure of GHR --- p.9
Chapter 1.2.5 --- The Structure of GHR Gene --- p.13
Chapter 1.2.6 --- Growth Hormone Binding Protein (GHBP) --- p.14
Chapter 1.2.7 --- The GH/Prolactin/Cytokine/Erythropoietin Receptor Superfamily --- p.15
Chapter 1.2.8 --- Proposed Signal Transduction Pathway --- p.17
Chapter 1.2.9 --- GHR Related Dwarfism --- p.22
Chapter i). --- Substitution of certain amino acid residues in the extracellular domain --- p.22
Chapter ii). --- Deletion of the extracellular domain --- p.23
Chapter a). --- deletion of a small portion of the binding protein
Chapter b). --- deletion of a large portion of the binding protein
Chapter c). --- deletion of a large portion of the binding domain and the whole transmembrane domain
Chapter iii). --- Associated with normal GHBP --- p.24
Chapter 1.3 --- Objectives of Cloning Vertebrate GHR cDNAs --- p.24
Chapter Chapter 2 --- General Experimental Methods
Chapter 2.1 --- Preparation of Ribonuclease Free Reagents and Apparatus --- p.26
Chapter 2.2 --- Isolation of Total RNA --- p.26
Chapter 2.3 --- Isolation of mRNA --- p.26
Chapter a). --- directly from tissue
Chapter b). --- from isolated total RNA
Chapter 2.4 --- Spectrophotometric Quantification and Qualification of DNA and RNA --- p.29
Chapter 2.5 --- First Strand cDNA Synthesis --- p.29
Chapter 2.6 --- Polymerase Chain Reaction (PCR) --- p.30
Chapter 2.7 --- Agarose Gel Electrophoresis --- p.31
Chapter 2.8 --- Formaldehyde Agarose Gel Electrophoresis of RNA --- p.31
Chapter 2.9 --- Capillary Transfer of DNA/RNA to a Nylon Membrane (Southern/Northern Blotting) --- p.32
Chapter a). --- DNA denaturing
Chapter b). --- Capillary transfer
Chapter 2.10 --- DNA Radiolabelling --- p.33
Chapter a). --- By random primer translation
Chapter b). --- By nick translation
Chapter 2.11 --- Spuncolumn Chromatography --- p.34
Chapter 2.12 --- Hybridization of Southern/Northern Blot --- p.35
Chapter 2.13 --- Autoradiography --- p.35
Chapter 2.14 --- Linearization and Dephosphorylation of Plasmid DNA --- p.36
Chapter 2.15 --- Restriction Digestion of DNA --- p.36
Chapter 2.16 --- Purification of DNA from Agarose Gel using GENECLEAN® Kit --- p.36
Chapter 2.17 --- 3' End Modification of PCR Amplified DNA --- p.37
Chapter 2.18 --- Ligation of DNA Fragments to Linearized Vector --- p.37
Chapter 2.19 --- Preparation of Escherichia coli Competent Cells --- p.38
Chapter 2.20 --- Transformation of the Escherichia coli Strain DH5a --- p.38
Chapter 2.21 --- Minipreparation of Plasmid DNA --- p.39
Chapter 2.22 --- DNA Purification by Phenol/Chloroform Extraction --- p.39
Chapter 2.23 --- Ethanol Precipitation of DNA and RNA --- p.40
Chapter 2.24 --- Preparation of Plasmid DNA using Wizard´ёØ Minipreps DNA Purification Kit from Promega --- p.40
Chapter 2.25 --- Preparation of Plasmid DNA using QIAGEN-tip100 --- p.41
Chapter 2.26 --- DNA Sequencing --- p.42
Chapter 2.26.1 --- DNA Sequencing Reaction
Chapter a). --- T7 sequencing
Chapter b). --- PCR sequencing
Chapter 2.26.2 --- DNA Sequencing Electrophoresis --- p.44
Chapter i). --- Preparation of 8% polyacrylamide gel solution
Chapter ii). --- Casting the gel
Chapter iii). --- Electrophoresis
Chapter Chapter 3 --- Molecular Cloning of Golden Hamster (Mesocricetus auratus) GHR cDNA
Chapter 3.1 --- Introduction --- p.46
Chapter 3.2 --- Experimental Methods
Chapter 3.2.1 --- Animals and Tissues --- p.47
Chapter 3.2.2 --- PCR Cloning of GHR cDNA Fragments in the Cytoplasmic Domain --- p.47
Chapter 3.2.2.1 --- Primer design and PCR strategy --- p.47
Chapter 3.2.2.2 --- PCR studies on the hamster liver and kidney first strand cDNA --- p.49
Chapter 3.2.2.3 --- Southern analysis of the PCR products --- p.50
Chapter 3.2.2.4 --- Subcloning and sequencing of PCR amplified cDNA fragments --- p.50
Chapter 3.2.3 --- Screening of a Hamster Liver cDNA Library --- p.51
Chapter 3.2.3.1 --- Preparation of the plating bacteria --- p.51
Chapter 3.2.3.2 --- Phage titering of the λ ZAP library --- p.51
Chapter 3.2.3.3 --- Primary screening of the amplified hamster liver cDNA library --- p.52
Chapter 3.2.3.4 --- Plaque uplifting and hybridization with hamster GHR cDNA fragment --- p.52
Chapter 3.2.3.5 --- Purification of putative clones from primary screening --- p.53
Chapter 3.2.3.6 --- Checking the size of the DNA insert --- p.53
Chapter 3.2.3.7 --- In vitro excision to release phagemid from the phage vector --- p.54
Chapter 3.2.3.8 --- Plasmid minipreparation of the putative clones --- p.56
Chapter 3.2.3.9 --- Nucleotide sequencing of the DNA inserts of different clones --- p.56
Chapter 3.2.4 --- Tissue Distribution of GHR in Hamster Tissues and the Relative Expression Level of GHR mRNAin these tissues --- p.58
Chapter 3.2.5 --- Cloning of the Full-length GHR cDNA into a Mammalian Vector --- p.59
Chapter 3.2.5.1 --- PCR amplification of the full-length hamster GHR cDNA --- p.59
Chapter 3.2.5.2 --- Preparation of the hamster GHR cDNA insert for ligation --- p.60
Chapter 3.2.5.3 --- Linearization of pRc/CMV expression vector --- p.60
Chapter 3.2.5.4 --- Ligation of the linearized expression vector with the full-length hamster GHR cDNA --- p.61
Chapter 3.3 --- Results
Chapter 3.3.1 --- PCR Amplification of Hamster GHR cDNA Fragments --- p.61
Chapter 3.3.1.1 --- RT-PCR --- p.61
Chapter 3.3.1.2 --- Southern blot analysis --- p.62
Chapter 3.3.1.3 --- Subcloning and nucleotide sequencing of PCR amplified hamster GHR cDNA fragments --- p.64
Chapter 3.3.2 --- Screening of an Amplified λZAP Hamster Liver cDNA Library --- p.70
Chapter 3.3.2.1 --- Preparation of the cDNA probe and phage titering --- p.70
Chapter 3.3.2.2 --- Screening of the cDNA library --- p.70
Chapter 3.3.2.3 --- PCR study of the 5' and 3' regions of the DNA insert of the clones selected for secondary screening --- p.72
Chapter 3.2.3.4 --- Nucleotide sequencing of the full-length hamster GHR cDNA --- p.73
Chapter 3.2.3.5 --- Tissue distribution of GHR in hamster and the relative expression level of the GHR mRNA in these tissues --- p.73
Chapter 3.2.3.6 --- Cloning of the full-length hamster GHR cDNA into a mammalian expression vector --- p.79
Chapter 3.4 --- Discussion
Chapter 3.4.1 --- Cloning of the Full-length hamster GHR cDNA --- p.81
Chapter 3.4.2 --- Comparison of the Nucleotide and the Predicted Amino Acid Sequences of the Hamster GHR with other Cloned GHRs --- p.82
Chapter 3.4.3 --- Tissue Distribution of GHR in Hamster and the Relative Expression Level of the GHR mRNA in these Tissues --- p.89
Chapter 3.4.4 --- Further Studies on Hamster GHR --- p.90
Chapter Chapter 4 --- Molecular Cloning of Chinese Bullfrog (Rana tigria rigulosa) GHR cDNA from Adult Frog Liver
Chapter 4.1 --- Introduction --- p.92
Chapter 4.2 --- Experimental Methods
Chapter 4.2.1 --- Animal and Tissues --- p.93
Chapter 4.2.2 --- Cloning of the Cytoplasmic Domain of Frog GHR cDNA by PCR --- p.93
Chapter 4.2.2.1 --- RT-PCR --- p.93
Chapter 4.2.2.2 --- Southern blot analysis of PCR amplified products --- p.95
Chapter 4.2.2.3 --- Subcloning and sequencing of PCR amplified DNA fragments --- p.95
Chapter 4.2.2.4 --- Restriction analysis of GHR cDNA fragment between GHR p1 and GHR p2 --- p.95
Chapter 4.2.2.5 --- PCR cloning of other portions of frog GHR cDNA --- p.96
Chapter 4.2.2.6 --- Subcloning and sequencing of PCR amplified GHR cDNA fragment using primers other than GHR p1 and GHR p2 --- p.97
Chapter 4.3 --- Results
Chapter 4.3.1 --- Cloning of the Intracellular Domain of Frog GHR cDNA by RT-PCR --- p.97
Chapter 4.3.1.1 --- RT-PCR --- p.97
Chapter 4.3.1.2 --- Southern blot analysis --- p.98
Chapter 4.3.1.3 --- Subcloning and sequencing of PCR amplified DNA fragments --- p.98
Chapter 4.3.1.4 --- Restriction enzyme analysis of GHR cDNA fragments --- p.102
Chapter 4.3.1.5 --- PCR cloning of other portions of frog GHR cDNA --- p.103
Chapter 4.3.1.6 --- Subcloning and sequencing of PCR products from other portions of frog GHR cDNA --- p.103
Chapter 4.4 --- Discussion
Chapter 4.4.1 --- Cloning of the Full-length frog GHR cDNA --- p.109
Chapter 4.4.2 --- Further Studies on Frog GHR --- p.117
Chapter Chapter 5 --- Attempts on the Molecular Cloning of Teleost GHR cDNA
Chapter 5.1 --- Introduction --- p.119
Chapter 5.2 --- Experimental Methods
Chapter 5.2.1 --- Animals and Tissues --- p.120
Chapter 5.2.2 --- PCR Cloning of Teleost GHR cDNA fragments --- p.120
Chapter 5.2.2.1 --- Design of PCR primers --- p.120
Chapter 5.2.2.2 --- Preparation of mRNA and synthesis of first strand cDNA --- p.122
Chapter 5.2.2.3 --- PCR studies on dace and snakehead fish liver first strand cDNA --- p.122
Chapter 5.2.2.3.1 --- PCR studies on dace liver first strand cDNA --- p.122
Chapter 5.2.2.3.2 --- PCR studies on snakehead fish liver first strand cDNA --- p.122
Chapter 5.2.3 --- "Northern Analysis on Dace, Snakehead fish and Eel mRNA" --- p.123
Chapter 5.3 --- Results
Chapter 5.3.1 --- Molecular Studies on Dace GHR cDNA --- p.123
Chapter 5.3.1.1 --- PCR studies on dace first strand cDNA --- p.123
Chapter 5.3.2 --- PCR Studies on Teleost First Strand cDNA --- p.128
Chapter 5.3.3 --- Northern Analysis on Teleost mRNA --- p.128
Chapter 5.4 --- Discussion --- p.130
Chapter 5.4.1 --- PCR Studies on Teleost GHR cDNA --- p.130
Chapter 5.4.2 --- Northern Analysis on Teleost mRNA --- p.131
Chapter Chapter 6 --- General Discussion
Chapter 6.1 --- Achievement of this Project --- p.134
Chapter 6.1.1 --- Hamster GHR --- p.134
Chapter 6.1.2 --- Frog GHR --- p.135
Chapter 6.1.3 --- Teleost GHR --- p.136
Chapter 6.2 --- Postulation on Cloned GHRs at the Molecular Level --- p.136
Bibliography --- p.141
Appendices --- p.150
"Exploring the Telomeric Repeat Addition Processivity of Vertebrate Telomerase." Doctoral diss., 2010. http://hdl.handle.net/2286/R.I.8590.
Повний текст джерелаDissertation/Thesis
Ph.D. Biochemistry 2010
Rocha, Susana F. 1980. "The role of multiple DSL ligands - DII1, DII4 and Jag1 - in vertebrate neurogenesis." Master's thesis, 2009. http://hdl.handle.net/10451/1159.
Повний текст джерелаDisponível no documento
Fundação para a Ciência e Tecnologia, BD/18745/2004 POCI 2010