Дисертації з теми "UNCOVER"
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Lynn, C. "Using adaptation to uncover motion processing mechanisms." Thesis, Queen's University Belfast, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.546383.
Повний текст джерелаvon, Mackensen Jana. "Thinking Friction : Uncover the true colours of Berlin." Thesis, KTH, Stadsbyggnad, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-100151.
Повний текст джерелаUtsunomiya, Yuri Tani [UNESP]. "Genome-wide scans to uncover loci underlying bovine reproductive biology." Universidade Estadual Paulista (UNESP), 2013. http://hdl.handle.net/11449/98165.
Повний текст джерелаO desempenho reprodutivo dos animais tem um grande impacto sobre a indústria da carne bovina. A caracterização de regiões genômicas que afetam a fertilidade dos animais pode contribuir para a identificação de marcadores preditivos de desempenho reprodutivo e desvendar os mecanismos moleculares envolvidos em aspectos complexos da biologia reprodutiva dos bovinos. Nos dois primeiros estudos relatados, os genomas de touros da raça Nelore (Bos indicus) foram examinados em busca de loci que explicam variação nas características peso ao nascer (PN) e perímetro escrotal (PE), utilizando dados de mais de 777.000 marcadores do tipo polimorfismo de sítio único (single nucleotide polymorphism - SNP). Um segmento do cromossomo 14, o qual engloba o gene ortólogo PLAG1 que afeta estatura em humanos, foi encontrado tanto em PN quanto em PE. Este locus possui efeitos pleiotrópicos sobre características reprodutivas e de tamanho corporal em bovinos, e representa um ponto de partida para a dissecção da genética da fertilidade bovina. Em outro estudo, um teste estatístico composto foi desenvolvido e aplicado na busca de evidências de assinaturas de seleção no genoma de raças bovinas de leite e de corte. Padrões de variação genética que podem ter sido moldadas pela seleção humana foram detectados no genoma de quatro diferentes raças bovinas (Angus, Pardo Suíço, Gir e Nelore). O estudo indica o gene Cornichon 3 (CNIH3) como um forte candidato, que pode estar envolvido na regulação do pico pré-ovulatório do hormônio luteinizante na raça Pardo-Suíço. Embora estes resultados apenas toquem a superfície dos mecanismos moleculares por trás da reprodução dos bovinos, os loci aqui identificados abrigam novos e conhecidos genes candidatos que afetam a fertilidade da espécie, e oferecem novas perspectivas sobre aspectos complexos de sua biologia reprodutiva
Reproductive performance has a high impact on the beef cattle industry. The characterization of genomic regions affecting fertility can contribute to the identification of diagnostic markers for reproductive performance and uncover molecular mechanisms underlying complex aspects of bovine reproductive biology. In the first two reported studies, the genomes of progeny-tested Nellore bulls (Bos indicus) were scanned for loci explaining variance in birth weight (BW) and scrotal circunferemce (SC), using data containing over 777,000 single nucleotide polymorphism (SNP) markers. Among the identified loci, a chromosome segment located on autosome 14, encompassing the orthologous human stature gene pleiomorphic adenoma 1 (PLAG1), was found to affect both BW and SC. This locus has been found to have pleiotropic effects on reproduction and body size traits in cattle, and represents a starting point to the dissection of the complex inheritance of bovine fertility. In a separate study, a composite statistical test was developed and applied to scan dairy and beef cattle genomes for evidences of natural and artificial selection signatures. Patterns of genetic variation that may have been shaped by human-driven selection were detected in the genomes of four different cattle breeds (Angus, Brown Swiss, Gyr and Nellore). The study pointed to the Cornichon homolog 3 gene (CNIH3) as a strong candidate involved in the regulation of pre-ovulatory luteinizing hormone surge in Brown Swiss. Although these findings only scratch the surface of the molecular mechanisms underlying bovine reproduction, the loci identified here harbor known and novel functional candidate genes affecting fertility in cattle and offer new insights on complex aspects of bovine reproductive biology
Ashwanden, Cordelia. "An ethnographic journey to uncover the culture of dialysis units." Thesis, University of Southampton, 2002. https://eprints.soton.ac.uk/50616/.
Повний текст джерелаUtsunomiya, Yuri Tani. "Genome-wide scans to uncover loci underlying bovine reproductive biology /." Jaboticabal, 2013. http://hdl.handle.net/11449/98165.
Повний текст джерелаCoorientador: Johann Sölkner
Banca: Joaquim Mansano Garcia
Banca: Adriana Santana do Carmo
Resumo: O desempenho reprodutivo dos animais tem um grande impacto sobre a indústria da carne bovina. A caracterização de regiões genômicas que afetam a fertilidade dos animais pode contribuir para a identificação de marcadores preditivos de desempenho reprodutivo e desvendar os mecanismos moleculares envolvidos em aspectos complexos da biologia reprodutiva dos bovinos. Nos dois primeiros estudos relatados, os genomas de touros da raça Nelore (Bos indicus) foram examinados em busca de loci que explicam variação nas características peso ao nascer (PN) e perímetro escrotal (PE), utilizando dados de mais de 777.000 marcadores do tipo polimorfismo de sítio único (single nucleotide polymorphism - SNP). Um segmento do cromossomo 14, o qual engloba o gene ortólogo PLAG1 que afeta estatura em humanos, foi encontrado tanto em PN quanto em PE. Este locus possui efeitos pleiotrópicos sobre características reprodutivas e de tamanho corporal em bovinos, e representa um ponto de partida para a dissecção da genética da fertilidade bovina. Em outro estudo, um teste estatístico composto foi desenvolvido e aplicado na busca de evidências de assinaturas de seleção no genoma de raças bovinas de leite e de corte. Padrões de variação genética que podem ter sido moldadas pela seleção humana foram detectados no genoma de quatro diferentes raças bovinas (Angus, Pardo Suíço, Gir e Nelore). O estudo indica o gene Cornichon 3 (CNIH3) como um forte candidato, que pode estar envolvido na regulação do pico pré-ovulatório do hormônio luteinizante na raça Pardo-Suíço. Embora estes resultados apenas toquem a superfície dos mecanismos moleculares por trás da reprodução dos bovinos, os loci aqui identificados abrigam novos e conhecidos genes candidatos que afetam a fertilidade da espécie, e oferecem novas perspectivas sobre aspectos complexos de sua biologia reprodutiva
Abstract: Reproductive performance has a high impact on the beef cattle industry. The characterization of genomic regions affecting fertility can contribute to the identification of diagnostic markers for reproductive performance and uncover molecular mechanisms underlying complex aspects of bovine reproductive biology. In the first two reported studies, the genomes of progeny-tested Nellore bulls (Bos indicus) were scanned for loci explaining variance in birth weight (BW) and scrotal circunferemce (SC), using data containing over 777,000 single nucleotide polymorphism (SNP) markers. Among the identified loci, a chromosome segment located on autosome 14, encompassing the orthologous human stature gene pleiomorphic adenoma 1 (PLAG1), was found to affect both BW and SC. This locus has been found to have pleiotropic effects on reproduction and body size traits in cattle, and represents a starting point to the dissection of the complex inheritance of bovine fertility. In a separate study, a composite statistical test was developed and applied to scan dairy and beef cattle genomes for evidences of natural and artificial selection signatures. Patterns of genetic variation that may have been shaped by human-driven selection were detected in the genomes of four different cattle breeds (Angus, Brown Swiss, Gyr and Nellore). The study pointed to the Cornichon homolog 3 gene (CNIH3) as a strong candidate involved in the regulation of pre-ovulatory luteinizing hormone surge in Brown Swiss. Although these findings only scratch the surface of the molecular mechanisms underlying bovine reproduction, the loci identified here harbor known and novel functional candidate genes affecting fertility in cattle and offer new insights on complex aspects of bovine reproductive biology
Mestre
Shires, Katherine L. "Using IEG's to uncover pathways for spatial learning in the rat." Thesis, Cardiff University, 2006. http://orca.cf.ac.uk/54312/.
Повний текст джерелаRogers, Robert. "Capturing moments : an investigation into how best to uncover teachers' knowledge." Thesis, University of East Anglia, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.365018.
Повний текст джерелаProudfoot, Jeffrey Gainer. "Identifying Deception Using Novel Technology-Based Approaches to Uncover Concealed Information." Diss., The University of Arizona, 2014. http://hdl.handle.net/10150/316781.
Повний текст джерелаSwanström, Emma. "That's when we uncover : En studie om jämställdhetsdebatten kring artisten Zara Larsson." Thesis, Umeå universitet, Institutionen för kultur- och medievetenskaper, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-119249.
Повний текст джерелаSwanström, Emma. "That's when we uncover : En analys av jämställdhetsdebatten kring artisten Zara Larsson." Thesis, Umeå universitet, Institutionen för kultur- och medievetenskaper, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-116185.
Повний текст джерелаCorsini, Julien. "Analysis and evaluation of network intrusion detection methods to uncover data theft." Thesis, Edinburgh Napier University, 2009. http://researchrepository.napier.ac.uk/output/4031/.
Повний текст джерелаSantos, Raquel Alexandra Martinho dos. "Novel functions of the cohesin accessory factor dPDS5 uncover a new meiotic checkpoint." Doctoral thesis, Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica, 2014. http://hdl.handle.net/10362/12072.
Повний текст джерелаMeiosis is a highly specialized type of cell division that is essential for sexual reproduction in all Eukaryotic species, where in two rounds of chromosome segregation take place without an intervening DNA replication phase (Petronczki et al., 2003). Genetic recombination during meiosis allows for the increase of genetic variability; furthermore, it is known that, at least in males, some level of chromatin reorganization occurs, such as histone displacement (White-Cooper and Davidson, 2011). Recombination requires the induction of endogenous Double Strand Breaks (DSBs), leading to the activation of a DNA Damage Response (DDR), that both recruits repair proteins and stalls the cell cycle until repair is completed (Harper and Elledge, 2007). Until now no surveillance pathway has been described that assures proper meiotic chromatin organization. In this work, we present evidence for the existence of such a surveying mechanism.(...)
Walters, Ashley Daniell. "Using multiple lines of evidence to uncover hidden biodiversity in desert spring ecosystems." Miami University / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=miami1533045376808751.
Повний текст джерелаMukherji, Proshanto. "Laws in logical worlds : using statistical inference to uncover the structure of logical domains /." Saarbrücken : VDM Verlag Dr. Müller, 2008. http://opac.nebis.ch/cgi-bin/showAbstract.pl?u20=9783836454377.
Повний текст джерелаKling, Leslie A. "Using metaphorical techniques in focus groups to uncover feelings of mothers toward family meals." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 85 p, 2008. http://proquest.umi.com/pqdweb?did=1459903861&sid=6&Fmt=2&clientId=8331&RQT=309&VName=PQD.
Повний текст джерелаJaishankar, Gayatri, and Deborah Thibeault. "Screen, Uncover, Connect: A Hands-on Approach to Elucidating the Social Determinants of Health." Digital Commons @ East Tennessee State University, 2017. https://dc.etsu.edu/etsu-works/8870.
Повний текст джерелаJaishankar, Gayatri, Deborah Thibeault, and Angelica Johnson. "Screen, Uncover, Connect: A Hands-on Approach to Elucidating the Social Determinants of Health." Digital Commons @ East Tennessee State University, 2017. https://dc.etsu.edu/etsu-works/8871.
Повний текст джерелаMukherji, Proshanto. "Laws in logical worlds using statistial inference to uncover the structure of logical domains." Saarbrücken VDM Verlag Dr. Müller, 2006. http://d-nb.info/989451313/04.
Повний текст джерелаStokes, Mindy. "Women, Domestic Abuse, And Dreams: Analyzing Dreams To Uncover Hidden Traumas And Unacknowledged Strengths." [Tampa, Fla.] : University of South Florida, 2004. http://purl.fcla.edu/fcla/etd/SFE0000425.
Повний текст джерелаBharathan, Navaneetha Krishnan. "USING THE FROG EPIDERMIS TO UNCOVER DESMOSOME FUNCTION AND REGULATION IN THE DEVELOPING EMBRYO." VCU Scholars Compass, 2018. https://scholarscompass.vcu.edu/etd/5313.
Повний текст джерелаFreidhoff, Paul. "Using RNA Mimicry of Viroids to Uncover New Noncoding RNA Structural Motifs and Pathways." Thesis, University of the Sciences in Philadelphia, 2020. http://pqdtopen.proquest.com/#viewpdf?dispub=27665966.
Повний текст джерелаYang, Qi. "A system biology approach to uncover the regulatory and effector hubs of Rac-family GTPases." Thesis, McGill University, 2014. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=121446.
Повний текст джерелаLes protéines de la famille Rho forment une branche majeure de la superfamille des petites GTPases Ras. Ces protéines peuvent être subdivisées en six sous-familles selon leur identité de séquence d'acides aminés primaires, leur motifs structuraux et leur fonction biologique, soient les sous-familles: Rho, Cdc42, Rac, RhoBTB, RhoT et Rnd. Les petites GTPases agissent comme des "interrupteurs moléculaires" soit qu'elles vont osciller entre un état "inactif" (GTPase-GDP) et un état "actif" (GTPase-GTP), et ce cycle est régulé par des facteurs d'échange de nucléotides guanine (GEFs) et des protéines activatrices de GTPases (GAPs). Les GTPases Rho ont une multitude d'effecteurs et elles sont impliquées dans de nombreux processus cellulaires et physiologiques, tels que la réorganisation du cytosquelette, la dynamique des microtubules, la polarité cellulaire, l'expression génique et la progression du cycle cellulaire. Cependant, il est encore mal compris comment les GTPases Rho sélectionnent des effecteurs spécifiques pour les différentes fonctions physiologiques. Nous émettons l'hypothèse que le choix de l'effecteur est dictée par la GEF impliquée ou par une protéine d'échafaudage qui est soit liée à la GEF ou à la GTPase. Pour tester cette hypothèse, j'ai utilisé une approche de la biologie des systèmes qui déterminera l'interactome complet des GTPases Rac (Rac1, Rac2, RAC3 et RhoG) dans leur conformation active et inactive. Grâce à cette méthode, nous avons essayé de découvrir des nouveaux effecteurs de Rac, des protéines d'échafaudage, ainsi que des GEFs et des GAPs spécifiques pour Rac.
Oakgrove, Francis. "Intellectual and Experiential Knowing: An Exploratory Factor Analysis to Uncover the Underlying Structure of Knowing." Diss., North Dakota State University, 2019. https://hdl.handle.net/10365/29886.
Повний текст джерелаAhmad, Fahmida. "Modelling heterogeneity of triple-negative breast cancer in mice to uncover and target signaling essentiality." Thesis, Aix-Marseille, 2020. http://www.theses.fr/2020AIXM0225.
Повний текст джерелаTriple-negative breast cancer (TNBC) is a highly aggressive, heterogeneous breast cancer subtype, and has currently no effective treatment. The aim of my PhD project was to understand the mechanisms triggering TNBC and to develop new therapeutic approaches.In our lab, we have generated a unique mouse model (MMTV-R26Met mice) in which a subtle increase in the expression levels of the wild-type MET receptor tyrosine kinase leads to spontaneous, exclusive TNBC formation. This model recapitulates formation of lung metastasis, resistance to conventional chemotherapeutic agents and to a set of combined targeted molecular therapies.Proteomic profiling of MMTV-R26Met tumors and machine learning approach showed that the MMTV-R26Met model largely recapitulates TNBC heterogeneity observed in TNBC patients. We identified two new drug combinations highly deleterious for the MMTV-R26Met tumor-derived cell lines, and a panel of human TNBC cells. The first drug combination targets the anti-apoptotic factor BCL-XL and CDK1/2 (cell cycle regulators). The second drug combination, validated by in vivo studies, is based on a combinatorial targeting of BCL-XL and of WEE1 (cell cycle and epigenetic regulator). Mechanistically, we show that combined inhibition of WEE1 and BCL-XL leads to DNA damage, premature entry into mitosis, resulting in mitotic catastrophe and apoptosis.Our findings may be highly relevant for their potential translation to the clinic, also in view of promising studies of monotherapy with BCL-XL and WEE1 inhibitors in phase II clinical trials
Baranowski, B. "Chemical screening to uncover small molecules that modulate neural stem cell self-renewal and differentiation." Thesis, University College London (University of London), 2015. http://discovery.ucl.ac.uk/1468463/.
Повний текст джерелаSánchez, Arroyo Alicia 1989. "Metabarcoding of the 18S rRNA gene to uncover new molecular biodiversity in Metazoa and unicellular Opisthokonta." Doctoral thesis, Universitat Pompeu Fabra, 2019. http://hdl.handle.net/10803/667270.
Повний текст джерелаUna de las principales transiciones evolutivas es el origen de la multicelularidad en animales y hongos a partir de sus ancestros unicelulares. Para entender este cambio es necesario estudiar a los organismos unicelulares más próximos evolutivamente a los animales (Metazoa) y hongos, formando todos ellos el clado Opisthokonta. Se ha comprobado que añadir información genética de más especies al árbol de los opistocontos puede cambiar drásticamente nuestra visión de dicha transición. Sin embargo, la diversidad real de estos linajes es prácticamente desconocida. Por tanto, el principal objetivo de este doctorado ha sido analizar la diversidad molecular de los opistocontos unicelulares y de animales para buscar novedad genética en forma de nuevos clados y, a la vez, entender su ecología. Usando datos de Secuenciación de Nueva Generación, principalmente metabarcoding del gen universal 18S rRNA, he realizado inferencias filogenéticas y ecológicas, junto con análisis de redes para descubrir nueva diversidad de organismos opistocontos desconocidos.
Paulson, Courtney L. "Using the Haar-Fisz wavelet transform to uncover regions of constant light intensity in Saturn's rings." Honors in the Major Thesis, University of Central Florida, 2010. http://digital.library.ucf.edu/cdm/ref/collection/ETH/id/1473.
Повний текст джерелаBachelors
Sciences
Statistics
Tafreshian, Kimia [Verfasser]. "A screen to uncover mediators of resistance to liver X receptor agonistic cancer therapy / Kimia Tafreshian." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2021. http://d-nb.info/1228859833/34.
Повний текст джерелаFrancisco, Rita. "Biochemistry of grape berries: post-genomics approaches to uncover the effects of water deficits on ripening." Doctoral thesis, Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica, 2011. http://hdl.handle.net/10362/6185.
Повний текст джерелаGrapevine (Vitis vinifera L.) is one of the most important fruit crops worldwide. In Europe, high-quality wine producing areas are traditionally non-irrigated. However, irrigation has become a wide-spread agronomical practice to overcome the deleterious effects of drought, high temperature and high evaporative demand that vines can be exposed to during the growing season. This is particularly true in the Mediterranean area, where the foreseen scenario predicts that water deficit may become a limiting factor in wine production and quality. Paradoxically, the imposition of mild to moderate water deficit (WD) via e.g. water deficit irrigation has been regarded as an agronomical tool that manipulates berry sensory characteristics, while maintaining yield. Grape berries, which are described as non-climacteric fruits, undergo a complex biochemical suite of alterations during development and ripening that remain poorly understood, including the molecular events that control the onset of ripening. At harvest, grape berry quality is largely dependent on the sugar/acids balance within the berry flesh, and on phenolic compounds (e.g. flavonoids) in the grape skin, which contribute to wine colour, aroma and flavour.(...)
PhD Fellowship SFRH/BD/30344/2006 from ‘Fundação para a Ciência e a Tecnologia’ (FCT) and ‘Fundo Social Europeu’ in the scope of ‘Quadro Comunitário de Apoio’.
Kent, Robyn. "Experimentally inducible gametocytogenesis : a new tool to uncover the early stages of commitment in P. berghei." Thesis, University of Glasgow, 2016. http://theses.gla.ac.uk/8135/.
Повний текст джерелаKarlsson, Karin, and Lamei Tang. "Consistency in Constant Change : A Sensemaking Perspective to Uncover How Marketers Justify Budget for Branding Activities." Thesis, Linköpings universitet, Företagsekonomi, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-177730.
Повний текст джерелаÇergani, Ervina [Verfasser], Mira Akademischer Betreuer] Mezini, and Christoph-Matthias [Akademischer Betreuer] [Bockisch. "Machine Learning as a Mean to Uncover Latent Knowledge from Source Code / Ervina Cergani ; Mira Mezini, Christoph Bockisch." Darmstadt : Universitäts- und Landesbibliothek Darmstadt, 2020. http://nbn-resolving.de/urn:nbn:de:tuda-tuprints-116586.
Повний текст джерелаÇergani, Ervina [Verfasser], Mira [Akademischer Betreuer] Mezini, and Christoph [Akademischer Betreuer] Bockisch. "Machine Learning as a Mean to Uncover Latent Knowledge from Source Code / Ervina Cergani ; Mira Mezini, Christoph Bockisch." Darmstadt : Universitäts- und Landesbibliothek Darmstadt, 2020. http://d-nb.info/1211088456/34.
Повний текст джерелаCergani, Ervina [Verfasser], Mira [Akademischer Betreuer] Mezini, and Christoph [Akademischer Betreuer] Bockisch. "Machine Learning as a Mean to Uncover Latent Knowledge from Source Code / Ervina Cergani ; Mira Mezini, Christoph Bockisch." Darmstadt : Universitäts- und Landesbibliothek Darmstadt, 2020. http://d-nb.info/1211088456/34.
Повний текст джерелаPlack, Leah. "Using the Task Analysis Process with Teachers to Uncover Language Demands within an Eight-Week NGSS Summer Course." PDXScholar, 2017. https://pdxscholar.library.pdx.edu/open_access_etds/4014.
Повний текст джерелаSoboleski, Penny K. "THE HIDDEN CHILDREN OF THE CLASSROOM: A VALIDATION STUDY USING ALTERNATIVE ASSESSMENTS TO UNCOVER THIRD-GRADE READERS WITH DYSLEXIA." Connect to this title online, 2006. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=bgsu1143482871.
Повний текст джерелаSuryavanshi, Gajendra [Verfasser], and Roland [Akademischer Betreuer] Eils. "Mathematical model to uncover the role of receptor ubiquitination in dose dependent EGFR trafficking / Gajendra Suryavanshi ; Betreuer: Roland Eils." Heidelberg : Universitätsbibliothek Heidelberg, 2012. http://d-nb.info/1179783727/34.
Повний текст джерелаWatts, Sarah. "Investigation to uncover the electrophysiological correlates of the mediating cognitive factors, responsible for the immediate emotional enhancement of memory." Thesis, Durham University, 2015. http://etheses.dur.ac.uk/11069/.
Повний текст джерелаSuryavanshi, Gajendra Wamanrao [Verfasser], and Roland [Akademischer Betreuer] Eils. "Mathematical model to uncover the role of receptor ubiquitination in dose dependent EGFR trafficking / Gajendra Suryavanshi ; Betreuer: Roland Eils." Heidelberg : Universitätsbibliothek Heidelberg, 2012. http://nbn-resolving.de/urn:nbn:de:bsz:16-opus-131084.
Повний текст джерелаRamos, Vicente David. "Phylogenetic Studies of Glutamate Receptors and their Auxiliary Subunits Update their Classifications and Uncover their Diverse Metazoan Evolutionary Histories." Doctoral thesis, Universitat Autònoma de Barcelona, 2021. http://hdl.handle.net/10803/673959.
Повний текст джерелаEl glutamato es el principal neurotransmisor excitatorio del sistema nervioso de los vertebrados e invertebrados. Las proteínas involucradas en la neurotransmisión glutamatérgica, y especialmente los receptores de glutamato y sus subunitades auxiliares, juegan un papel clave en el funcionamiento del sistema nervioso. Así, entender su evolución y revelar su diversidad es esencial para comprender como ha evolucionado el sistema nervioso, dando forma a la función cognitiva. El análisis integral de la filogenia de estas proteínas en los metazoos ha revelado que su evolución es mucho más compleja de lo que se podia anticipar en base al genoma de los vertebrados. Esto es particularmente cierto para los receptores ionotrópicos de glutamato, pues su clasificación actual en seis clases (AMPA, Kainato, Delta, NMDA1, NMDA2 and NMDA3) estaría altamente incompleta. El trabajo aquí presentado propone una clasificación en 4 subfamilias que engloban 10 clases. Los receptores AMPA, Kainato y Delta de vertebrados pertenecerían a una de estas subfamilias, llamada AKDF, y las subunidades NMDA constituirían otra subfamilia. Además, también podrían existir dos subfamilias no descritas previamente, que son referidas como Epsilon y Lambda. Por otro lado, las familias de proteínas que contienen subunidades auxiliares de receptores AMPA (ARAS) han experimentado historias evolutivas menos complejas. No obstante, los vertebrados habrían reclutado para actuar como ARAS a la sinapsis proteínas de estas familias mediante procesos de neo y/o subfuncionalización que se dieron después de eventos de duplicación génica ocurridos en este linaje. Así este trabajo favorece la hipótesis de que la complejidad del sistema nervioso podría no haber evolucionado incrementando el conjunto de receptores de neurotransmisores en el genoma, sino augmentando la regulación de estos receptores en la sinapsis.
Glutamate is the major excitatory neurotransmitter in vertebrate and invertebrate nervous systems. Proteins involved in glutamatergic neurotransmission, and chiefly glutamate receptors and their auxiliary subunits, play key roles in nervous system function. Thus, understanding their evolution and uncovering their diversity is essential to comprehend how nervous systems evolved, shaping cognitive function. Comprehensive phylogenetic analysis of these proteins across metazoans have revealed that their evolution is much more complex than what can be anticipated from vertebrate genomes. This is particularly true for ionotropic glutamate receptors, as their current classification in six classes (AMPA, Kainate, Delta, NMDA1, NMDA2 and NMDA3) would be largely incomplete. New work proposes a classification into 4 subfamilies that encompass 10 classes. Vertebrate AMPA, Kainate and Delta receptors would belong to one of these subfamilies, named AKDF, and the NMDA subunits would constitute another subfamily. Furthermore, two previously unreported subfamilies would also exist, these are referred to as Epsilon and Lambda. On the other hand, protein families containing AMPA receptor auxiliary subunits (ARAS) have experienced less complex evolutionary histories. Nevertheless, vertebrates would have recruited to function as ARAS in the synapse proteins from these families by neo and/or subfunctionalization after gene duplication events occurred in this lineage. Thus, this work favours the hypothesis that nervous system complexity could have evolved not by increasing the set of neurotransmitter receptors in the genome, but by increasing the regulation of such receptors in the synapse.
Universitat Autònoma de Barcelona. Programa de Doctorat en Neurociències
Camprubí, Font Carla. "Genetics and transcriptomics of adherent-invasive Escherichia coli (AIEC): new approaches to uncover molecular markers for its rapid identification." Doctoral thesis, Universitat de Girona, 2019. http://hdl.handle.net/10803/672302.
Повний текст джерелаEl patotip adherent-invasiu d’Escherichia coli (AIEC) podria jugar un paper en el transcurs de la malaltia de Crohn. Aquest es caracteritza per tenir capacitat d’adhesió i invasió a cèl·lules de l’epiteli intestinal a més de replicar-se i sobreviure en macròfags. Actualment la única manera d’identificar aquests bacteris és analitzant aquestes característiques fenotípiques, un mètode poc estandarditzat i que requereix molt temps i dedicació. En la present tesi ens hem centrat en estudiar genèticament el patotip AIEC per tal de buscar característiques clau que puguin ajudar en el desenvolupament d’una eina molecular per a la seva identificació. En resum, els resultats d'aquest treball proporcionen informació significativa que contribueix a la comprensió de la genètica del patotip AIEC. En aquest cas, s'han presentat dos possibles marcadors moleculars resultants d'una combinació de característiques genètiques i/o fenotípiques que podrien ajudar en la detecció d’AIEC. Finalment, els resultats d'expressió gènica proporcionen noves idees per descriure millor els gens implicats en la virulència del patotip AIEC
Programa de Doctorat en Biologia Molecular, Biomedicina i Salut
Luippold, Benjamin Labrie. "Managing audits to manage earnings the impact of baiting tactics on an auditor's ability to uncover earnings management errors /." Amherst, Mass. : University of Massachusetts Amherst, 2009. http://scholarworks.umass.edu/open_access_dissertations/106/.
Повний текст джерелаVan, de Velde Caroline. "Monitoring eye movements to uncover the role of visual context in the access to verb-complement information during sentence comprehension." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/MQ64022.pdf.
Повний текст джерелаMerz, Dale R. Jr. "Molecular simulations uncover the nanomechanics of heat shock protein (70 kDa) & Indentation simulations of microtubules reveal katanin severing insights." University of Cincinnati / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1583154342504106.
Повний текст джерелаStewart, Michelle Elizabeth. "Development of high-throughput, non-invasive behavioural and cognitive tests in mice to uncover new mechanisms of abnormal cognition and behaviour." Thesis, Open University, 2018. http://oro.open.ac.uk/58323/.
Повний текст джерелаPrice, David Andrew. "Using Infrared Spectroscopy to Uncover Structure in Biomolecular Assemblies Related to Disease: Applications to Nucleic Acid and Peptide Oligomers and Aggregates." OpenSIUC, 2020. https://opensiuc.lib.siu.edu/dissertations/1845.
Повний текст джерелаBrown, Donna Noelle. "Facilitating changes in the practice context: using action research to uncover the significance of psychological safety - an example from pain management with older people." Thesis, Ulster University, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.490391.
Повний текст джерелаDupont, Marie. "Étude fonctionnelle de gènes candidats impliqués dans les ciliopathies Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion." Thesis, Sorbonne Paris Cité, 2019. http://www.theses.fr/2019USPCB006.
Повний текст джерелаCiliopathies are multisystemic disorders due to mutations in genes encoding proteins localizing at the cilium. Cilia are microtubule-based organelles present at the surface of almost all vertebrate cells. IFT52 is a key component of the intraflagellar transport IFT-B complex that guarantees cilium formation and functions. Missense, nonsense mutations and in-frame deletions have been identified in IFT52 in three families presenting distinct phenotypes of ciliopathies: multicystic kidney dysplasia (MCKD) or skeletal dysplasia such as short ribs-polydactyly (SRP) or Sensenbrenner (SB). To understand the phenotype variability, we sought to characterize the pathogenicity of the mutations by using patient fibroblasts and CRISPR/Cas9-induced cellular and animal models. First, we showed that the nature of the in-frame (SRP) and nonsense mutations (SB) actually led to a frameshift and an in- frame exon skipping, respectively that partially explained the genotype/phenotype correlation. On the contrary, analyses of missense mutations from SRP and MCDK cases indicated that SRP mutation had a more severe impact on IFT52 function than the DRK mutation, confirming the pathogenicity of the mutations and explaining the skeletal defects of SRP case. However, it did not explain the renal phenotype variability observed amongst the individuals. Thus, we considered a second homozygous missense mutation in the MCDK case in UBE2C, a gene encoding an ubiquitin conjugating enzyme involved in metaphase/anaphase transition. Tridimensionnal modelisation of the protein structure showed that the mutation affected the binding with ubiquitin and likely the function of the protein. In vitro studies confirmed that the mutation delayed mitosis exit. In order to study the role of Ube2c in kidney development, a knock-in (KI) mouse line of the patient mutation was generated by CRISPR/Cas9 technique. Although Ube2cKI/KI failed to thrive and died early, similarly to the knock-out line, kidneys appeared normal in these animals. So we hypothesized thet the renal phenotype of the MCDK case could come from a synergy between the mutations in IFT52 and UBE2C. To test this hypothesis, we performed synergy experiments by injecting ift52 morpholino in zebrafish embryos issued from ube2c+/- incrosses, but we did not observe kideny defects in ube2c-/- embryos. Thus, we conclude that either the MCDK individual present another mutation responsible for the renal phenotype or the animal models we used (mouse, zebrafish) were not appropriate to study the involvement of UBE2C in human kidney development. Surprisingly, the study on IFT52 mutations highlighted an abnormal splitting of the centrioles of the same centrosome in Ift52-/- cells. Centrosome cohesion is guaranteed by two mechanisms: a protein linker between proximal parts of the centrioles and the microtubule (MTs) forces exerted on the centrosome. The proteins from the protein linker, such as c-Nap1 and Rootletin, did not seem to be affected by the loss of Ift52. However, we showed that Ift52 interacted and partially co-localized with centrin at the distal part of the centriole, suggesting a role for Ift52 in the MTs mechanism. Indeed, we showed that in Ift52-/- cells, MTs presented failed to anchor at the centrosome and their dynamic was reduced. These defects could be the cause of the centrosome splitting and suggest an extra-ciliary role for IFT52 in MTs anchoring and centrosome cohesion, as its partner IFT88 in mitosis, adding another physiopathological mechanism to the IFT52-associated ciliopathies
Jia, Zhongtao [Verfasser], Nicolaus von [Gutachter] Wirén, Klaus [Gutachter] Humbeck, and Malcolm [Gutachter] Bennett. "Exploiting natural variation to uncover genes modulating root foraging responses to low nitrogen in Arabidopsis thaliana / Zhongtao Jia ; Gutachter: Nicolaus Wirén, Klaus Humbeck, Malcolm Bennett." Halle (Saale) : Universitäts- und Landesbibliothek Sachsen-Anhalt, 2019. http://d-nb.info/1220288144/34.
Повний текст джерелаMastrogiovanni, Gianmarco. "Establishment of new human and mouse liver cancer models and their use to uncover the role of RNF43 and ZNRF3 in liver homeostasis and repair." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/273341.
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