Дисертації з теми "Tuberculosis markers"
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся з топ-21 дисертацій для дослідження на тему "Tuberculosis markers".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Переглядайте дисертації для різних дисциплін та оформлюйте правильно вашу бібліографію.
Montalvo, R., D. Kirwan, R. Gilman, and Antonio Bernabe-Ortiz. "Bioimpedance markers and tuberculosis outcome among HIV-infected patients." Obafemi Awolowo University, 2018. http://hdl.handle.net/10757/624732.
Повний текст джерелаRevisión por pares
Felix, Alvina Clara. "Estudo da resposta imunológica de anticorpos IgG, IgM e IgA, subclasses de IgG (IgG1 e IgG3) e avidez de IgG, por Western Blotting, em amostras de soros de pacientes com tuberculose pulmonar e comparação de resultados com métodos microbiológicos e dosagem de interferon-gama." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/5/5134/tde-21072011-135809/.
Повний текст джерелаDespite the recommendations of the World Health Organization, during the Ministerial meeting held in Amsterdam, Holland in 2000, tuberculosis continues at an important increasing rate, affecting mostly developing countries and people with severe compromised immune systems, especially those infected with human acquired immunodeficiency virus. The methods used for diagnosis are the same used for many years, whose limitations prevent the rapid action of countries health programs that seek to stop the chain of disease transmission. Continuing a line of research of the Laboratory of Seroepidemiology and Immunobiology of IMTSP using the method of Western blotting, in this study we tried to broaden the knowledge of the immune response of antibodies in patients with pulmonary tuberculosis, clinical and laboratory defined by evaluating the participation of IgG, IgA and IgM, IgG subclasses (IgG1 and IgG3) and immunoglobulin IgG avidity in serum samples collected in the beginning and end of treatment. Our results showed that the immunoglobulin IgG was best immunological marker when compared with the results of microbiological methods and determination of interferon gamma, QuantiFERON TB Gold. The proteins fractions that showed better diagnosis performance were 38 and 30 KDa
Dreesman, Alexandra. "Development and evaluation of new diagnostic markers of tuberculosis in children." Doctoral thesis, Universite Libre de Bruxelles, 2020. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/312223.
Повний текст джерелаDoctorat en Sciences médicales (Médecine)
info:eu-repo/semantics/nonPublished
Kremer, Kristin. "Genetic markers for Mycobacterium tuberculosis characterization and spread of the Beijing genotype /." [S.l. : Amsterdam : s.n.] ; Universiteit van Amsterdam [Host], 2005. http://dare.uva.nl/document/78818.
Повний текст джерелаVan, der Spuy Gian Dreyer. "Analysis and application of evolutionary markers in the epidemiology of Mycobacterium tuberculosis." Thesis, Stellenbosch : Stellenbosch University, 2008. http://hdl.handle.net/10019.1/1457.
Повний текст джерелаThis series of studies includes both methodological analyses, aimed at furthering our understanding of, and improving the tools used in molecular epidemiology, and investigative projects which have used these tools to add to our knowledge of the M. tuberculosis epidemic. Using serial isolates from tuberculosis patients, we have investigated the evolutionary rate of the IS6110 RFLP pattern. In accordance with other studies, we determined a ½-life for this epidemiological marker of 10.69 years, confirming its appropriateness for this purpose. We also identified an initial, much higher apparent rate which we proposed was the result of pre-diagnostic evolution. In support of this, our investigations in the context of household transmission of M. tuberculosis revealed that IS6110-based evolution is closely associated with transmission of the organism, resulting in a strain population rate of change of 2.9% per annum. To accommodate evolution within estimates of transmission, we proposed that calculations incorporate the concept of Nearest Genetic Distance (cases most similar in RFLP pattern and most closely associated in time). We used this to create transmission chains which allowed for limited evolution of the IS6110 marker. As a result, in our study community, the estimated level of disease attributable to ongoing transmission was increased to between 73 and 88% depending on the Genetic Distance allowed. We identified the duration of a study as a further source of under-estimation of transmission. This results from the artefactual abridgement of transmission chains caused by the loss of cases at the temporal boundaries of a study. Using both real and simulated data, we showed that viewing a 12-year study through shorter window periods dramatically lowered estimates of transmission. This effect was negatively correlated with the size of a cluster. Various combinations of MIRU-VNTR loci have been proposed as an alternative epidemiological marker. Our investigations showed that, while this method yielded estimates of transmission similar to those of IS6110, there was discordance between the two markers in the epidemiological linking of cases as a result of their independent evolution. Attempting to compensate for this by allowing for evolution during transmission improved the performance of IS6110, but generally had a deleterious effect of that of MIRU-VNTR. However, this marker remains a valuable tool for higher phylogenetic analysis and we used it to demonstrate a correlation between sublineages of the Beijing clade and the regions in which they are found. We proposed that, either the host population had selected for a particular sublineage, or that specific sublineages had adapted to be more successful in particular human populations. We further explored the dynamics of the epidemic over a 12-year period in terms of the five predominant M. tuberculosis clades. We found that, while four of these clades remained relatively stable, the incidence of cases from the Beijing clade increased exponentially. This growth was attributed to drug-sensitive cases although drug-resistant Beijing cases also appeared to be more successful than their non-Beijing counterparts. Possible factors contributing to this clade’s success were a greater proportion of positive sputum smears and a lower rate of successful treatment.
Phillips, Patrick Peter John. "Prognostic and surrogate markers for outcome in the treatment of pulmonary tuberculosis." Thesis, London School of Hygiene and Tropical Medicine (University of London), 2009. http://researchonline.lshtm.ac.uk/1544172/.
Повний текст джерелаJhilmeet, Nishtha. "Immunological markers of protective immune reconstitution in HIV infected persons sensitised by Mycobacterium tuberculosis." Doctoral thesis, University of Cape Town, 2018. http://hdl.handle.net/11427/28420.
Повний текст джерелаSloan, Derek. "Investigation into mycobacterial persistence and early markers of outcome in the treatment of pulmonary tuberculosis." Thesis, University of Liverpool, 2013. http://livrepository.liverpool.ac.uk/12873/.
Повний текст джерелаMenezes, Angela Maria. "Utilization of antigen-specific host responses in the evaluation of Mycobacterium tuberculosis infection, development of disease and treatment effect." Thesis, Stellenbosch : Stellenbosch University, 2013. http://hdl.handle.net/10019.1/79850.
Повний текст джерелаENGLISH ABSTRACT: Setting This study was conducted in the Tygerberg district, Cape Town, in the Western Cape, South Africa Background The evaluation of early tuberculosis (TB) treatment response is based on month 2 sputum culture status. This method of evaluation has a number of limitations: the test requires relatively advanced laboratory infrastructure and procedures, it takes several weeks to obtain results and is a relatively a poor marker at predicting treatment response. The discovery of potential host markers which reflect the efficacy of early treatment would be of great importance for clinical management of individual patients. The treatment failure would be detectable earlier than at week 8 of treatment. The duration of clinical trials of new anti-tuberculosis drugs may also be substantially reduced by such markers if these would be measurable earlier than at week 8 of therapy. Objectives 1) To evaluate diluted, 7-day whole blood cultures stimulated with live Mycobacterium tuberculosis (M.tb) for the presence of host markers of early TB treatment response 2) To evaluate an overnight, undiluted, M.tb antigen stimulated whole blood culture Quantiferon Gold In Tube (QFT-GIT) supernatants for host markers of early TB treatment response The study designs were as follows: In study one, baseline samples and samples from week 1, week 2 and week 4 of treatment from 30 cured TB patients were selected from a larger biomarker study, in which whole blood was stimulated with live M.tb or left unstimulated. Fifty seven host markers were measured in supernatants by multiplex cytokine arrays. In study two, baseline samples and samples from week 2 and week 8 of treatment from 19 cured TB patients were randomly selected from the placebo group in a micronutrient supplement study. QFT-GIT supernatants from these participants were assessed through multiplex cytokine arrays for levels of fifty seven host markers. All of the participants in both studies were Human Immunodeficiency Virus (HIV) negative. Changes in marker expression over time and between fast and slow responders to treatment were evaluated. Comparability between the two culture methods was assessed for markers that were evaluated in both studies. Results In study one, the majority of host markers showed significant changes over time in the unstimulated supernatants. Only GRO and IL-1beta changed significantly in an antigen-specific manner (background levels subtracted). No significant changes were observed between fast and slow responders. In study two, the majority of host markers showed significant changes over time in the unstimulated supernatants whereas only MDC and IL-4 changed during the observation period in antigen stimulated levels. Significant differences were observed between fast and slow responders at pre-treatment for IL-13 Ag-Nil and IL-1betaAg-Nil . Conclusion This study revealed, antigen-specific responses showed only limited potential for early TB treatment response monitoring, but may have potential in differentiating between treatment outcomes. Future investigations may have to include later time points during treatment as these were not included in the present assessment. The QFT-GIT samples do not appear to be equivalent to live M.tb stimulated 7-day whole blood assays.
AFRIKAANSE OPSOMMING: Instelling Die studie is uitgevoer in die Tygerbergdistrik, Kaapstad, Wes-Kaap, Suid-Afrika. Agtergrond Die evaluering van die respons op vroeë tuberkulose (TB) behandeling word gebaseer op die status van maand 2 sputum kulture. Hierdie evalueringsmetode het ‘n paar beperkinge: die toets benodig relatief gevorderde laboratorium infrastruktuur en prosedures, die toetsuitslae is eers na ‘n paar weke beskikbaar en dit is n relatiewe swak merker om repons op behandeling te voorspel. Die ontdekking van potensiële selfmerkers wat die doeltreffendheid van vroeë behandeling weerspieël sal van groot belang wees vir die kliniese bestuur van individuele pasiënte. Mislukking van die behandeling sal sodoende voor week 8 van behandeling waargeneem kan word. Die tydsduur van kliniese proewe van nuwe anti-tuberkulose medikasie mag ook baie verkort word met sulke merkers as dit voor week 8 van behandeling gemeet kan word. Doelwitte 1) Om verdunde, 7-dae oue volbloedkulture, met lewende Mikobakterium tuberkulosis (M.tb) gestimuleer, te evalueer vir die teenwoordigheid van vroeë TB behandeling respons selfmerkers. 2) Om die supernatant van oornag, onverdunde, M.tb antigeen gestimuleerde volbloedkulture Quantiferon Gold In Tube (QFT-GIT) vir vroeë behandeling respons selfmerkers te evalueer. Die studie-ontwerpe was soos volg: Met studie een is basislynmonsters en monsters verkry na week 1, week 2 en week 4 van behandeling van 30 geneesde TB-pasiënte geselekteer uit ‘n groter biomerkerstudie waarin die volbloed met lewende M.tb gestimuleer is of ongestimuleer gelaat is. Sewe-en-vyftig selfmerkers is in die supernatante gemeet deur middel van multipleks sitokine arrays. Met studie twee is basislynmonsters en monsters verkry na week 2 en week 8 van behandeling van 19 geneesde TB-pasiënte lukraak uit die plasebo-groep in ‘n mikrovoedingstowwe-aanvullingstudie geselekteer. Vlakke van 57 selfmerkers is in die QFT-GIT supernatante van hierdie deelnemers, deur middel van die multipleks sitokine arrays, bepaal. Al die deelnemers van beide studies was HIV negatief. Veranderinge in merker-uitdrukking oor tyd, asook tussen vinnige en stadige respons tot behandeling, is ge-evalueer. Die vergelykbaarheid van die twee kultuurmetodes is geassesseer ten opsigte van die ge-evalueerde merkers in albei studies. Resultate Met studie een het die meerderheid van die selfmerkers in die ongestimuleerde supernatante kenmerkende verandering oor tyd gewys. Slegs GRO en IL-1beta het aansienlik verander in die antigeenspesifieke wyse (agtergrond vlakke afgetrek). Geen kenmerkende veranderinge was waargeneem tussen die vinnige en stadige respons pasiënte nie. Met studie twee het die meerderheid van die selfmerkers aansienlike veranderinge oor tyd in die ongestimuleerde supernatante gewys, in vergelyking waar net die MDC en IL-4 veranderinge gedurende die observasie periode in antigeen gestimuleerde vlakke getoon het. Kenmerkende verskille is tussen die vinnige en stadige respons pasiënte in voorbehandeling vir IL-13 Ag-Nil en IL-1betaAg-Nil waargeneem. Gevolgtrekking Die studie bewys dat antigeenspesifieke response slegs beperkte potensiaal vir vroeë TB behandeling respons monitering het, maar mag die potensiaall vir onderskeidende behandeling uitkomste hê. Toekomstige ondersoeke sal dalk latere tydpunte gedurende die behandeling moet insluit aangesien dit nie in hierdie evaluasie ingesluit is nie. Die QFT-IT monsters verskyn nie as gelykwaardig met die lewendig M.tb gestimuleerde 7-dae volbloed toetse nie.
Rozhitskii, M. M., and O. A. Sushko. "Nanophotonic sensors for biomedical and ecological application." Thesis, B. Verkin Institute of Low Temperature Physics and Engineering, NASU, 2013. http://openarchive.nure.ua/handle/document/8873.
Повний текст джерелаStreicher, Elizabeth Maria. "Application of spoligotyping in the understanding of the dynamics of Mycobacterium tuberculosis strains in high incidence communities." Thesis, University of Stellenbosch. Faculty of Health Sciences. Dept. of Biomedical Sciences, 2007. http://hdl.handle.net/10019.1/1496.
Повний текст джерелаMatodzi, Unarine. "Evaluation of single nucleotide polymorphisms in virulence genes of Mycobacterium tuberculosis as markers of lineages and sub-lineages in Tshwane region." Diss., University of Pretoria, 2020. http://hdl.handle.net/2263/75329.
Повний текст джерелаDissertation (MSc)--University of Pretoria, 2020.
The thesis/Dissertation is under embargo until September 2023.
National Research Foundation (NRF)
The thesis is under embargo until September 2022.
Medical Microbiology
MSc
Unrestricted
Nouhin, Janin. "Roles of cellular innate immunity and inflammatory markers in the immune reconstitution syndrome observed during co-infection with tuberculosis in HIV infected patients in Cambodia." Thesis, Sorbonne Paris Cité, 2016. http://www.theses.fr/2016USPCC330.
Повний текст джерелаSimultaneous anti-tuberculosis and antiretroviral (ARY) therapy in HIV and tuberculosis (TB) co-infected patients can be complicated due to the occurrence of TB-associated immune reconstitution inflammatory syndrome (TB-IRIS). The diagnosis test of TB-IRIS is not yet available and mainly based on clinical data. A better understanding of TB-IRIS immunopathology is crucial to improve diagnostic test and patients’ clinical outcomes. Innate immunity seems increasingly play a role in TB-IRIS. In the present doctoral thesis, is studied the role of cellular innate immunity, including NKT and γδ t cells, and as well as the implication of IL-1Ra, sCD14 and sCD163 plasma soluble markers related to the activation of monocytes/macrophages in the development of iris in HIV and TB co-infected patients in Cambodia. The results have shown that 1/. TB-IRIS is associated with a strong activation of γδ t cells and γδ2+ subset before initiation of ARY, 2/. None of IL-1Ra, sCD14 and sCD163 markers was predictive of the onset of iris. Longitudinal analysis of IL-1Ra plasma level could be useful for the diagnosis of the iris occurrence and for the evaluation of response to TB-IRIS In conclusion, our results reveal the association between important activation of innate immunity and the emergence of TB-IRIS in the physiopathology. In addition, our data provides new element of TB-IRIS and markers for evaluation of TB treatment efficacy
Bekele, Adane Mihret. "Gene expression and cytokine pattern of pulmonary tuberculosis patients and their contacts in Ethiopia." Thesis, Stellenbosch : Stellenbosch University, 2012. http://hdl.handle.net/10019.1/71942.
Повний текст джерелаENGLISH ABSTRACT: The immune response against M. tuberculosis is multifactorial, involving a network of innate and adaptive immune responses. Characterization of the immune response, a clear understanding of the dynamics and interplay of different arms of the immune response and the identification of infection-stage specific biomarkers are critical to allow the development of better tools for combating tuberculosis. In an attempt to identify such biomarkers, we studied pulmonary tuberculosis patients and their contacts in Addis Ababa, Ethiopia as part of EDCTP and BMGF funded tuberculosis projects by using multiplex techniques. We analysed 45 genes using the Multiplex Ligation Dependent Probe Amplification (MLPA) technique and the expression of IL-4δ2, BLR1, MARCO, CCL-19, IL7R, Bcl2, FcyR1A, MMP9, and LTF genes discriminate TB cases from their healthy contacts. FoxP3, TGFß1 and CCL-19 discriminate latently infected from uninfected contacts. Single genes predict with an area under the Receiver Operating Characteristic (ROC) curve of 0.68 to 0.85 while a combination of genes identified up to 95% of the different groups. Similarly, the multiplex analysis of cytokines and chemokines also showed that single or combinations of plasma cytokines and chemokines discriminate between different clinical groups accurately. The median plasma level of EGF, fractalkine, IFN-y, IL-4, MCP-3 and IP-10 is significantly different (p<0.05) in active tuberculosis and non active tuberculosis infection and the median plasma levels of IFN-y, IL-4, MCP-3, MIP-1ß and IP-10 were significantly different (p<0.05) before and after treatment. We also found a significant difference (p<0.05) in plasma levels of cytokines of patients infected with the different lineages and different families of the modern lineage. The plasma level of IL-4 was significantly higher in patients infected with lineage 3 (p<0.05) as compared to lineage 4 and the CAS familyinfected patients had a higher plasma level of IL-4 (P<0.05) as compared to patients infected with H and T families but there was no difference between H and T families. We identified genes and cytokines which had been reported from other studies in different settings and we believe that these molecules are very promising biomarkers for classifying active tuberculosis, latent infection, absence of infection and treated infection. These markers may be suitable for the development of clinically useful tools but require further validation and qualification in different populations and in larger studies.
AFRIKAANSE OPSOMMING: Die immuunrespons teen M. tuberculosis is multifaktoriaal en betrek ‘n netwerk van niespesifieke and spesifieke immuunresponse. Karakterisering van die immuunrespons, ‘n duidelike insig in die dinamika en tussenspel deur die verskillende arms van die immuunrespons en die identifikasie van spesifieke biomerkers is krities belangrik om die ontwikkeling van nuwe hulpmiddels teen tuberkulose te bevorder. In ‘n poging om sulke biomerkers te identifiseer het ons pulmonale tuberkulose pasiënte en hulle kontakte in Addis Ababa, Etiopië, as deel van die EDCTP en BMGF befondste tuberkulose projekte bestudeer met multipleks tegnieke. Ons het 45 gene analiseer met ‘Multiplex Ligation Dependent Probe Amplification (MLPA)’ en gevind dat die geenuitdrukking van IL-4•2, BLR1, MARCO, CCL-19, IL7R, Bcl2, Fc•R1A, MMP9, en LTF TB pasiënte van hulle kontakte onderskei. FoxP3, TGF•1 en CCL-19 onderskei tussen latent infekteerde en ongeïnfekteerde kontakte. Enkele gene voorspel met ‘n area onder die ‘Receiver Operating Characteristic (ROC)’ kurwe van 0.68 tot 0.85 terwyl die kombinasie van gene 95% van die verskillende groepe identifiseer. Soortgelyk het multipleks analise van sitokiene en chemokiene verskillende kliniese groepe akkuraat van mekaar onderskei. Die mediane plasmavlakke van EGF, fractalkine, IFN-•, IL-4, MCP-3 en IP-10 is beduidend verskillend (p<0.05) in aktiewe tuberkulose en nie-aktiewe tuberkulose infeksie en die mediane plasmavlak van IFN-•, IL-4, MCP-3, MIP-1• en IP-10 was beduidend verskillend voor en na behandeling. Ons het ook beduidende verskille (p<0.05) in plasmavlakke van sitokiene in pasiënte gevind wat infekteer is met verskillende stamme and verskillende families van die moderne stamme. Die plasmavlak van IL-4 was beduidend hoër in pasiënte wat infekteer is met stam 3 (p<0.05) teenoor stam 4 en die CAS familie-infekteerde pasiënte het ‘n hoër plasmavlak van IL-4 (p<0.05) teenoor pasiënte met H en T familie infeksie hoewel daar geen versikke was tussen die H en T families nie. Ons het gene en sitokiene identifiseer wat deur ander werkers onder verskillende omstandighede ook beskryf is en ons glo dat hierdie molekules baie belowende biomerkers is om aktiewe tuberkulose, latent tuberkulose, die afwesigheid van infeksie en behandelde infeksie van mekaar te onderskei. Hierdie merkers mag toepaslik wees vir die ontwikkeling van bruikbare kliniese hulpmiddele maar benodig verdere validasie en kwalifikasie in verskillende populasiegroepe en in groter studies.
Bill and Melinda Gates Foundation (BMGF)
European and Developing Countries Clinical Trials Partnership (EDCTP)
African European Tuberculosis Consortium (AE TBC).
Rossouw, Ingrid. "The intra- and inter-population relatedness of bovine tuberculosis-infected and -uninfected African buffaloes (Syncerus caffer caffer) in the Kruger National Park." Diss., University of Pretoria, 2010. http://hdl.handle.net/2263/25714.
Повний текст джерелаDissertation (MSc)--University of Pretoria, 2010.
Production Animal Studies
unrestricted
Kamfer, Fanie. "Characterising tuberculosis treatment success and failure using metabolomics / Fanie Kamfer." Thesis, North-West University, 2013. http://hdl.handle.net/10394/10203.
Повний текст джерелаMSc (Biochemistry), North-West University, Potchefstroom Campus, 2013
Moriconi, Patrícia Rossi. "Pesquisa de Mycobacterium spp. em queijos minas meia cura obtidos em feiras-livres da cidade de São Paulo." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-04092014-142755/.
Повний текст джерелаMycobacterium genus consists of saprophytic and pathogenic microorganisms of interest in animal and human health. M. bovis specie, which causes tuberculosis in animals, is excreted through the milk of infected cattle and the consumption of raw milk and its derivatives is an important route of transmission to humans, causing a disease as serious as the one caused by M. tuberculosis. Considering that the disease in animals is endemic in Brazil and that minas meia cura cheese cure is usually made from raw milk and much appreciated by paulistano consumer, samples of the product acquired in open markets, were analyzed for the occurrence of mycobacteria. The samples were decontaminated by the method HPC 1.5%, sown in Stonebrink-Leslie medium (incubated at 37 ° C/90 da ys) and the suspected colonies, submitted to PCR TB multiplex and nucleotide sequencing reaction. In 12% of samples (16/133) were isolated 26 colonies of Mycobacterium spp. and 6 species have been identified, all of them are environmental Mycobacterium fortuitum, M. confluentis, M. elephantis, M. novocastrense, M. sphagni and M. arupense; 7 isolates, however, remained without characterization for the specie. M. fortuitum is an important opportunistic pathogen in public health, without, however, evidence of being transmitted by food; M. novocastrense, M. arupense and M. elephantis species have also been considered potentially pathogenic to humans. The results suggest that the frequency and/or contamination load of M. bovis in meia cura Minas cheese is (are) low (s). We have to consider, however, that this result may have been influenced by the absence of an analytical method capable of identifying the agent in the food matrix in which a low load of microorganism is expected, accompanied by high load of contaminants. Also suggest the need to evaluate the possible importance of foodborne non-tuberculous mycobacteria, especially for immunocompromised individuals.
Kim, Nari. "A Novel Approach for Detection of Several Tuberculosis Markers Using Diffractive Optics." Thesis, 2011. http://hdl.handle.net/1807/27345.
Повний текст джерелаMlambo, Mbuso. "Design and development of multifunctional Raman active noble metals nanoprobes for the detection of malaria and tuberculosis biomarkers." Thesis, 2016. http://hdl.handle.net/10539/19299.
Повний текст джерелаSurface enhanced Raman spectroscopy (SERS) has emerged as a surface sensitive vibrational technique that leads to the enhancement of the Raman scattering molecules on or close to the surface of a plasmonic nanostructure. The enhancement is found to be in orders of 104 to 1015, which allows the technique to be sensitive enough to detect a single molecule. In this study, we report on the synthesis of different sizes of gold and silver nanoparticles (AuNPs and AgNPs) and gold nanorods (AuNRs). These are functionalized or co-stabilized with different stoichiometric ratios of HS-(CH2)11-PEG-COOH and alkanethiols (Raman reporters), i.e.; HS-(CH2)11-NHCO-coumarin(C), HS-(CH2)11-triphenylimidazole (TPI), HS- (CH2)11-indole (HSI), HS-(CH2)11-hydroquinone (HQ) to form mixed monolayer protected clusters (MMPCs). The alkanethiols were chosen as Raman reporters to facilitate the selfassembled formation of monolayers on the metal surface, thus resulting in stable MMPCs. The optical properties and stability of MMPCs were obtained using ultraviolet-visible (UVvis) spectrophometry and a zeta sizer. Size and shape of the as-synthesized nanoparticles were obtained using transmission electron microscopy (TEM). The tendency of thiolcapped nanoparticles to form self-assembled ordered superlattices was observed. Their Raman activities were evaluated using Raman spectroscopy, with the enhancement factor (EF) being calculated from the intensities of symmetric stretch vibrations of C-H observed in the region of about 2900 to 3000 cm-1 in all SERS spectra. In all four different alkanethiols (Raman reporters), smaller size metal nanoparticles (14 nm for AuNPs and 16 nm AgNPs) showed higher EF compared to 30 and 40 nm metal nanoparticles. The EF was observed to increase proportionally with stoichiometric ratios of alkanethiols from 1% iv | P a g e to 50%. The prepared MMPCs with small sizes were used as a SERS probe for the detection of malaria and tuberculosis biomarkers.
Daffy, Joanna. "Spoligotyping and pncA sequencing as an epidemiological marker for Pyrazinamide resistant M. Tuberculosis." Thesis, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-205439.
Повний текст джерелаBorderie, Léa Camille. "Alterações bioquímicas nas doenças infeciosas." Master's thesis, 2021. http://hdl.handle.net/10284/10880.
Повний текст джерелаThis dissertation aims to understand and identify the main biochemical alterations that occur in infectious diseases, namely in COVID-19, AIDS, tuberculosis, infections with Helicobacter pylori, Tinea corporis and toxoplasmosis. In this sense, the recognition of possible biochemical markers of each disease was highlighted with how they can be used in diagnosis. Additionally, the treatments and drugs used in each pathology were identified, as well as the molecules that are available in the market. A literature review was carried out in order to analyze the current state of knowledge in this area, the existing gaps and understanding how science is evolving in the development of the topic. Thus, the alterations and biochemical markers most used for the diagnosis of infectious diseases were identified. C reactive protein, interleucin-6, procalcitonin, lactate dehydrogenase, electrolytes, urease and immunoglobulins integrate some of the most important ones. It was concluded that biomarkers, due to the identified potentialities, should be used in clinical practice and be an integral part of the diagnosis of various pathologies. Among the potentialities, the capacity to identify risks and propensity to a disease occurrence, the capacity to stratify patients and to identify the severity and/or progression of a certain pathology, prognostic prediction and aptitude to monitor a certain treatment are highlighted.