Статті в журналах з теми "Tripartite GFP"
Щоб переглянути інші типи публікацій з цієї теми, перейдіть за посиланням: Tripartite GFP.
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся з топ-50 статей у журналах для дослідження на тему "Tripartite GFP".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Переглядайте статті в журналах для різних дисциплін та оформлюйте правильно вашу бібліографію.
1
Finnigan, Gregory C., Angela Duvalyan, Elizabeth N. Liao, Aspram Sargsyan, and Jeremy Thorner. "Detection of protein–protein interactions at the septin collar inSaccharomyces cerevisiaeusing a tripartite split-GFP system." Molecular Biology of the Cell 27, no. 17 (September 2016): 2708–25. http://dx.doi.org/10.1091/mbc.e16-05-0337.
Анотація:
Various methods can provide a readout of the physical interaction between two biomolecules. A recently described tripartite split-GFP system has the potential to report by direct visualization via a fluorescence signal the intimate association of minimally tagged proteins expressed at their endogenous level in their native cellular milieu and can capture transient or weak interactions. Here we document the utility of this tripartite split-GFP system to assess in living cells protein–protein interactions in a dynamic cytoskeletal structure—the septin collar at the yeast bud neck. We show, first, that for septin–septin interactions, this method yields a robust signal whose strength reflects the known spacing between the subunits in septin filaments and thus serves as a “molecular ruler.” Second, the method yields little or no spurious signal even with highly abundant cytosolic proteins readily accessible to the bud neck (including molecular chaperone Hsp82 and glycolytic enzyme Pgk1). Third, using two proteins (Bni5 and Hsl1) that have been shown by other means to bind directly to septins at the bud neck in vivo, we validate that the tripartite split-GFP method yields the same conclusions and further insights about specificity. Finally, we demonstrate the capacity of this approach to uncover additional new information by examining whether three other proteins reported to localize to the bud neck (Nis1, Bud4, and Hof1) are able to interact physically with any of the subunits in the septin collar and, if so, with which ones.
2
Sicard, Mathieu, Karine Brugirard-Ricaud, Sylvie Pag�s, Anne Lanois, Noel E. Boemare, Michel Breh�lin, and Alain Givaudan. "Stages of Infection during the Tripartite Interaction between Xenorhabdus nematophila, Its Nematode Vector, and Insect Hosts." Applied and Environmental Microbiology 70, no. 11 (November 2004): 6473–80. http://dx.doi.org/10.1128/aem.70.11.6473-6480.2004.
Анотація:
ABSTRACT Bacteria of the genus Xenorhabdus are mutually associated with entomopathogenic nematodes of the genus Steinernema and are pathogenic to a broad spectrum of insects. The nematodes act as vectors, transmitting the bacteria to insect larvae, which die within a few days of infection. We characterized the early stages of bacterial infection in the insects by constructing a constitutive green fluorescent protein (GFP)-labeled Xenorhabdus nematophila strain. We injected the GFP-labeled bacteria into insects and monitored infection. We found that the bacteria had an extracellular life cycle in the hemolymph and rapidly colonized the anterior midgut region in Spodoptera littoralis larvae. Electron microscopy showed that the bacteria occupied the extracellular matrix of connective tissues within the muscle layers of the Spodoptera midgut. We confirmed the existence of such a specific infection site in the natural route of infection by infesting Spodoptera littoralis larvae with nematodes harboring GFP-labeled Xenorhabdus. When the infective juvenile (IJ) nematodes reached the insect gut, the bacterial cells were rapidly released from the intestinal vesicle into the nematode intestine. Xenorhabdus began to escape from the anus of the nematodes when IJs were wedged in the insect intestinal wall toward the insect hemolymph. Following their release into the insect hemocoel, GFP-labeled bacteria were found only in the anterior midgut region and hemolymph of Spodoptera larvae. Comparative infection assays conducted with another insect, Locusta migratoria, also showed early bacterial colonization of connective tissues. This work shows that the extracellular matrix acts as a particular colonization site for X. nematophila within insects.
3
Pedelacq, Jean-Denis, and Stéphanie Cabantous. "Development and Applications of Superfolder and Split Fluorescent Protein Detection Systems in Biology." International Journal of Molecular Sciences 20, no. 14 (July 15, 2019): 3479. http://dx.doi.org/10.3390/ijms20143479.
Анотація:
Molecular engineering of the green fluorescent protein (GFP) into a robust and stable variant named Superfolder GFP (sfGFP) has revolutionized the field of biosensor development and the use of fluorescent markers in diverse area of biology. sfGFP-based self-associating bipartite split-FP systems have been widely exploited to monitor soluble expression in vitro, localization, and trafficking of proteins in cellulo. A more recent class of split-FP variants, named « tripartite » split-FP, that rely on the self-assembly of three GFP fragments, is particularly well suited for the detection of protein–protein interactions. In this review, we describe the different steps and evolutions that have led to the diversification of superfolder and split-FP reporter systems, and we report an update of their applications in various areas of biology, from structural biology to cell biology.
4
Barthe, Lucie, Vanessa Soldan, and Luis F. Garcia-Alles. "Assessment of oligomerization of bacterial micro-compartment shell components with the tripartite GFP reporter technology." PLOS ONE 18, no. 11 (November 27, 2023): e0294760. http://dx.doi.org/10.1371/journal.pone.0294760.
Анотація:
Bacterial micro-compartments (BMC) are complex macromolecular assemblies that participate in varied metabolic processes in about 20% of bacterial species. Most of these organisms carry BMC genetic information organized in operons that often include several paralog genes coding for components of the compartment shell. BMC shell constituents can be classified depending on their oligomerization state as hexamers (BMC-H), pentamers (BMC-P) or trimers (BMC-T). Formation of hetero-oligomers combining different protein homologs is theoretically feasible, something that could ultimately modify BMC shell rigidity or permeability, for instance. Despite that, it remains largely unknown whether hetero-oligomerization is a widespread phenomenon. Here, we demonstrated that the tripartite GFP (tGFP) reporter technology is an appropriate tool that might be exploited for such purposes. Thus, after optimizing parameters such as the size of linkers connecting investigated proteins to GFP10 or GFP11 peptides, the type and strength of promoters, or the impact of placing coding cassettes in the same or different plasmids, homo-oligomerization processes could be successfully monitored for any of the three BMC shell classes. Moreover, the screen perfectly reproduced published data on hetero-association between couples of CcmK homologues from Syn. sp. PCC6803, which were obtained following a different approach. This study paves the way for mid/high throughput screens to characterize the extent of hetero-oligomerization occurrence in BMC-possessing bacteria, and most especially in organisms endowed with several BMC types and carrying numerous shell paralogs. On the other hand, our study also unveiled technology limitations deriving from the low solubility of one of the components of this modified split-GFP approach, the GFP1-9.
5
Jahns, Anika C., and Bernd H. A. Rehm. "Tolerance of the Ralstonia eutropha Class I Polyhydroxyalkanoate Synthase for Translational Fusions to Its C Terminus Reveals a New Mode of Functional Display." Applied and Environmental Microbiology 75, no. 17 (July 6, 2009): 5461–66. http://dx.doi.org/10.1128/aem.01072-09.
Анотація:
ABSTRACT Here, the class I polyhydroxyalkanoate synthase (PhaC) from Ralstonia eutropha was investigated regarding the functionality of its conserved C-terminal region and its ability to tolerate translational fusions to its C terminus. MalE, the maltose binding protein, and green fluorescent protein (GFP) were considered reporter proteins to be translationally fused to the C terminus. Interestingly, PhaC remained active only when a linker was inserted between PhaC and MalE, whereas MalE was not functional. However, the extension of the PhaC N terminus by 458 amino acid residues was required to achieve a functionality of MalE. These data suggested a positive interaction of the extended N terminus with the C terminus. To assess whether a linker and/or N-terminal extension is generally required for a functional C-terminal fusion, GFP was fused to the C terminus of PhaC. Both fusion partners were active without the requirement of a linker and/or N-terminal extension. A further reporter protein, the immunoglobulin G binding ZZ domain of protein A, was translationally fused to the N terminus of the fusion protein PhaC-GFP and resulted in a tripartite fusion protein mediating the production of polyester granules displaying two functional protein domains.
6
Koraïchi, Faten, Rémi Gence, Catherine Bouchenot, Sarah Grosjean, Isabelle Lajoie-Mazenc, Gilles Favre, and Stéphanie Cabantous. "High-content tripartite split-GFP cell-based assays to screen for modulators of small GTPase activation." Journal of Cell Science 131, no. 1 (November 30, 2017): jcs210419. http://dx.doi.org/10.1242/jcs.210419.
7
Liu, Tzu‐Yin, Wen‐Chun Chou, Wei‐Yuan Chen, Ching‐Yi Chu, Chen‐Yi Dai, and Pei‐Yu Wu. "Detection of membrane protein–protein interaction in planta based on dual‐intein‐coupled tripartite split‐ GFP association." Plant Journal 94, no. 3 (March 23, 2018): 426–38. http://dx.doi.org/10.1111/tpj.13874.
8
Turnquist, Emily, Madison N. Schrock, Megan Halloran, and Gregory C. Finnigan. "Characterization of Septin Protein Interactions at the Yeast Bud Neck Using a Tripartite Split GFP Detection System." Microscopy and Microanalysis 24, S1 (August 2018): 1348–49. http://dx.doi.org/10.1017/s1431927618007225.
9
Agarwal, Neeraj, Sebastien Rinaldetti, Bassem B. Cheikh, Qiong Zhou, Evan P. Hass, Robert T. Jones, Molishree Joshi, et al. "TRIM28 is a transcriptional activator of the mutant TERT promoter in human bladder cancer." Proceedings of the National Academy of Sciences 118, no. 38 (September 13, 2021): e2102423118. http://dx.doi.org/10.1073/pnas.2102423118.
Анотація:
Bladder cancer (BC) has a 70% telomerase reverse transcriptase (TERT or hTERT in humans) promoter mutation prevalence, commonly at −124 base pairs, and this is associated with increased hTERT expression and poor patient prognosis. We inserted a green fluorescent protein (GFP) tag in the mutant hTERT promoter allele to create BC cells expressing an hTERT-GFP fusion protein. These cells were used in a fluorescence-activated cell sorting–based pooled CRISPR-Cas9 Kinome knockout genetic screen to identify tripartite motif containing 28 (TRIM28) and TRIM24 as regulators of hTERT expression. TRIM28 activates, while TRIM24 suppresses, hTERT transcription from the mutated promoter allele. TRIM28 is recruited to the mutant promoter where it interacts with TRIM24, which inhibits its activity. Phosphorylation of TRIM28 through the mTOR complex 1 (mTORC1) releases it from TRIM24 and induces hTERT transcription. TRIM28 expression promotes in vitro and in vivo BC cell growth and stratifies BC patient outcome. mTORC1 inhibition with rapamycin analog Ridaforolimus suppresses TRIM28 phosphorylation, hTERT expression, and cell viability. This study may lead to hTERT-directed cancer therapies with reduced effects on normal progenitor cells.
10
Hwang, Moonsun, Jae-kyun Ko, Noah Weisleder, Hiroshi Takeshima, and Jianjie Ma. "Redox-dependent oligomerization through a leucine zipper motif is essential for MG53-mediated cell membrane repair." American Journal of Physiology-Cell Physiology 301, no. 1 (July 2011): C106—C114. http://dx.doi.org/10.1152/ajpcell.00382.2010.
Анотація:
We recently discovered that MG53, a muscle-specific tripartite motif (TRIM) family protein, functions as a sensor of oxidation to nucleate the assembly of cell membrane repair machinery. Our data showed that disulfide bond formation mediated by Cys242 is critical for MG53-mediated translocation of intracellular vesicles toward the injury sites. Here we test the hypothesis that leucine zipper motifs in the coiled-coil domain of MG53 constitute an additional mechanism that facilitates oligomerization of MG53 during cell membrane repair. Two leucine zipper motifs in the coiled-coil domain of MG53 (LZ1 - L176/L183/L190/V197 and LZ2 - L205/L212/L219/L226) are highly conserved across the different animal species. Chemical cross-linking studies show that LZ1 is critical for MG53 homodimerization, whereas LZ2 is not. Mutations of the conserved leucines into alanines in LZ1, not in LZ2, diminish the redox-dependent oligomerization of MG53. Live cell imaging studies demonstrate that the movement of green fluorescent protein (GFP)-tagged MG53 mutants (GFP-LA1 and GFP-LA2) is partially compromised in response to mechanical damage of the cell membrane, and the GFP-LA1/2 double mutant is completely ineffective in translocation toward the injury sites. In addition to the leucine zipper-mediated intermolecular interaction, redox-dependent cross talk between MG53 appears to be an obligatory step for cell membrane repair, since in vivo modification of cysteine residues with alkylating reagents can prevent the movement of MG53 toward the injury sites. Our data show that oxidation of the thiol group of Cys242 and leucine zipper-mediated interaction among the MG53 molecules both contribute to the nucleation process for MG53-mediated cell membrane repair.
11
Omokehinde, Tolu, Steven Soll, and Paul Bieniasz. "The application of clustered regularly interspaced short palindromic repeats genome engineering to the study of host antiviral factors (VIR9P.1160)." Journal of Immunology 194, no. 1_Supplement (May 1, 2015): 215.26. http://dx.doi.org/10.4049/jimmunol.194.supp.215.26.
Анотація:
Abstract Human immunodeficiency virus (HIV), is a retrovirus that infects cells of the human immune system, including CD4+ T cells, macrophages and dendritic cells. Previously, two of the candidate genes identified showed a capacity to protect cells from HIV-1 infection were Tripartite motif containing 56 (TRIM56) and 2’, 3’-cyclic-nucleotide 3’-phosophodiesterase (CNP). Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-associated (CAS) protein 9 is a nuclease system involved in defense against invading phages and plasmids in bacteria. This system can be programmed to induce DNA double stranded breaks (DSBs), which when targeting coding regions of genes can create frame shift insertion/deletion (indel) mutations that result in gene knock-out cell lines. Using the CRISPR/Cas9 system, we were able to clone guide sequences targeting TRIM56 and CNP into a LentiCRISPR and LentiGuide plasmid. The cloned plasmids were then used to generate viruses that would deliver the necessary machinery to target and cleave TRIM56 and CNP genes. To test the efficiency of the CRISPR/Cas9 system, guide sequence targeting GFP were cloned. GFP deletion was observed in a 293T-GFP stable cell line and knockout efficiency was determined by analysis. Knockout results of TRIM56 and CNP are still pending, but we expect that TRIM56 and CNP deleted cell lines will demonstrate the contribution of each of these innate immune factors to the interferon response to HIV-1.
12
Uchida, Naoya, Matthew M. Hsieh, Aylin C. Bonifacino, Sandra D. Price, Allen E. Krouse, Mark E. Metzger, Robert E. Donahue та John F. Tisdale. "TRIM5α Variation Affects Lentiviral Transduction Efficiency for Long-Term Hematopoietic Repopulating Cells in a Rhesus Stem Cell Transplantation Model". Blood 118, № 21 (18 листопада 2011): 2056. http://dx.doi.org/10.1182/blood.v118.21.2056.2056.
Анотація:
Abstract Abstract 2056 The Tripartite Motif-containing protein 5 α (TRIM5α) is thought to play an important role in restricting retroviral infection among species in nature, and restricts HIV-1 based lentiviral transduction of rhesus hematopoietic repopulating cells. We previously developed a chimeric HIV-1 based lentiviral vectors (χHIV vectors) which includes a simian immunodeficiency virus (SIV) capsid in place of the HIV-1 capsid to circumvent this restriction (J Virol. 2009). Transduction efficiency, however, remained highly variable between animals. In this study, we sought to evaluate the effects of rhesus TRIM5α polymorphisms on lentiviral transduction of rhesus hematopoietic repopulating cells. To evaluate whether rhesus TRIM5α polymorphisms influence the efficiency of lentiviral transduction, we transduced cell lines expressing 6 different rhesus TRIM5α types (Mamu-1, -2, -3, -4, -5, and TrimCyp) with eGFP-encoding HIV1, χHIV, and SIV vectors (Figure). Among all TRIM5α cell lines, transgene expression rates (%GFP) from the χHIV vector fell between that of the HIV-1 vector and that of the SIV vector. For the χHIV and SIV vectors, transduction efficiency was reduced in Mamu-1, -2, and -3 (p<0.01) expressing cell lines when compared to that of control cells. For the HIV-1 vector, there was a reduction in %GFP among all TRIM5α types (p<0.01). These results suggest that both the χHIV and SIV vectors escape restriction through rhesus TRIM5α Mamu-4, -5, and TrimCyp. We then analyzed 16 rhesus macaques who were transplanted with CD34+ cells transduced with the χHIV vector. We evaluated %GFP in granulocytes and lymphocytes 6 months after transplantation, as %GFP expression has been found to plateau in the peripheral blood at 6 months. Transduction efficiency of rhesus CD34+ cells was also evaluated in vitro at the time of transplant based on %GFP expression. For statistical analysis, we assessed factors that potentially affect transduction efficiency, including age, sex, weight, total number of mobilized CD34+ cells, cytokine mobilization regimen (G-CSF & stem cell factor (SCF) vs. G-CSF & plerixafor), cell density during transduction, and TRIM5α polymorphisms. Multivariable analysis demonstrated that TRIM5α type Mamu-4 (50.9±19.0% vs. 26.6±16.7%, p=0.04) as well as mobilization regimen (48.5±17.4% vs. 12.7±7.1%, p=0.01) affected CD34+ cell transduction efficiency in vitro. TRIM5α Mamu-4 only showed significant effects on %GFP among lymphocytes in vivo (23.7±17.9% vs. 5.3±3.1%, p=0.046). When analyzing the %GFP among granulocytes in vivo, there was a significant correlation with weight (p=0.01), mobilized CD34+ cell number (p=0.02), TRIM5α type Mamu-4 (29.3±25.4% vs. 8.4±6.0%, p=0.03), and mobilization regimen (25.4±24.1% vs. 7.5±3.0%, p=0.04). If in vitro %GFP is included in the analysis, both %GFP among granulocytes and lymphocytes are strongly affected (<0.001) and TRIM5α type Mamu-4 and mobilization regimen are no longer significant. Univariate analysis showed similar tendencies regarding %GFP among granulocytes and lymphocytes. Taken together, our data suggest that TRIM5α type Mamu-4, mobilization regimen (G-CSF & SCF), and CD34+ cell transduction efficiency in vitro are important factors that can predict higher %GFP in granulocytes and lymphocytes 6 months following transplantation. In summary, rhesus TRIM5α polymorphisms (especially type Mamu-4) play an important role in allowing efficient lentiviral transduction of long-term repopulating cells and contribute to the variable results observed in the rhesus hematopoietic stem cell gene therapy model. Disclosures: No relevant conflicts of interest to declare.
13
Van der Reijden, Bert A., Jurgen A. F. Marteijn, Liesbeth Van Emst, Theo De Witte, and Joop H. Jansen. "The Ubiquitin Ligase Triad1 Is Involved in Myelopoiesis and Interacts with the Transcriptional Repressors Gfi1 and Gfi1b." Blood 104, no. 11 (November 16, 2004): 64. http://dx.doi.org/10.1182/blood.v104.11.64.64.
Анотація:
Abstract We identified Triad1 as a gene that is upregulated by retinoic acid during the granulocytic differentiation of acute promyelocytic leukemia cells. In normal hematopoiesis, we show that Triad1 is weakly expressed in immature CD34+ bone marrow cells, and highly expressed in mature monocytes and granulocytes. Together, this suggests that Triad1 plays a role in the differentiation of hematopoietic cells. Triad1 contains a tripartite domain including two RING fingers, indicating that this protein might function as a ubiquitin E3 ligase, catalyzing the the conjugation of ubiquitin to substrate proteins thereby marking them for targeted degradation by the 26S proteasome. Using GST pull down experiments, we show that Triad1 binds to the ubiquitin conjugating (E2) enzymes UbcH6 and 7. In addition, immunoprecipitation of Triad1 in cells that were transfected with FLAG-tagged ubiquitin shows that Triad1 binds to ubiquitinated proteins, and that Triad1 is capable of self-ubiquitination, further corroborating the assumption that Triad1 acts as a E3 ubiquitin ligating enzyme. To study the role of Triad1 in hematopoiesis we overexpressed the gene in primary murine bone marrow cells using a retroviral vector that contains Triad1 in front of an IRES-GFP cassette. GFP positive cells were FACS sorted and used in colony assays (CFU-GM). Compared to empty vector controls (GFP alone), Triad1 expression resulted in more than 80% inhibition of clonogenic growth. Importantly, addition of the proteasome inhibitor MG132 (10E-8 M) reversed the Triad1-induced suppression of colony formation. Furthermore, three Triad1 expression constructs in which one of the conserved cys/his residues of the TRIAD domain (essential for function) were mutated did not show the suppressive effect on colony formation. Together, these data show that Triad1 is involved in myelopoiesis and acts through the ubiquitination of specific substrate proteins. To identify these substrates, a yeast-two-hybrid screen of a human bone marrow cDNA library was performed using the Triad1 protein as a bait. Interestingly, the transcriptional repressor Gfi1b was found to bind to Triad1. The interaction was confirmed by immunoprecipitation using GFP-Triad1 and FLAG-tagged Gfi1b transfections in mammalian cells. We show that Triad1 binds to the zinc finger region of Gfi1b. This region is very (>98%) homologous to the paralogue Gfi1. Further immunoprecipitation analyses showed that Triad1 also binds to the zinc finger region of Gfi1. Gfi1 plays an essential role in neutrophil development and Gfi1 pointmutations result in neutropenia in man. Currently, we are studying the direct ubiquitination of Gfi and Gfi1b by Triad1 in in vitro ubiquitination assays. In addition, we are studying the effect of Triad1 on the transcriptional repression of the ELA2 and other promoters by Gfi1.
14
Hatlem, Daniel, Thomas Trunk, Dirk Linke, and Jack C. Leo. "Catching a SPY: Using the SpyCatcher-SpyTag and Related Systems for Labeling and Localizing Bacterial Proteins." International Journal of Molecular Sciences 20, no. 9 (April 30, 2019): 2129. http://dx.doi.org/10.3390/ijms20092129.
Анотація:
The SpyCatcher-SpyTag system was developed seven years ago as a method for protein ligation. It is based on a modified domain from a Streptococcus pyogenes surface protein (SpyCatcher), which recognizes a cognate 13-amino-acid peptide (SpyTag). Upon recognition, the two form a covalent isopeptide bond between the side chains of a lysine in SpyCatcher and an aspartate in SpyTag. This technology has been used, among other applications, to create covalently stabilized multi-protein complexes, for modular vaccine production, and to label proteins (e.g., for microscopy). The SpyTag system is versatile as the tag is a short, unfolded peptide that can be genetically fused to exposed positions in target proteins; similarly, SpyCatcher can be fused to reporter proteins such as GFP, and to epitope or purification tags. Additionally, an orthogonal system called SnoopTag-SnoopCatcher has been developed from an S. pneumoniae pilin that can be combined with SpyCatcher-SpyTag to produce protein fusions with multiple components. Furthermore, tripartite applications have been produced from both systems allowing the fusion of two peptides by a separate, catalytically active protein unit, SpyLigase or SnoopLigase. Here, we review the current state of the SpyCatcher-SpyTag and related technologies, with a particular emphasis on their use in vaccine development and in determining outer membrane protein localization and topology of surface proteins in bacteria.
15
Heinzelmann Portella de Aguiar, Mônica. "Tripartite periodization of the Antarctic Treaty System from a systemic perspective." Relaciones Internacionales 28, no. 56 (August 8, 2019): 37–56. http://dx.doi.org/10.24215/23142766e054.
Анотація:
In 1959, twelve countries with a strong record of interest in Antarctica signed a Treaty allowing accessibility to all signatories wishing to conduct peaceful scientific research. The Antarctic Treaty established science and international cooperation as its cornerstones but raised controversies because of its hosting of sovereignty claims over Antarctic territory. This research aims to fill a gap in literature proposing a strict periodization of the Antarctic Treaty System under a systemic perspective. The paper also examines Brazil's accession into the Treaty. Using as indicators of legitimacy the increase in membership and the transparency of decision-making processes, the author argues that the Antarctic Treaty System has become recognized as a legitimate international regime.
16
Kolář, Petr, and Vladimír Svoboda. "How Rescher Failed to Fill the Fact/Value Gap." FILOSOFIE DNES 10, no. 1 (April 13, 2019): 4–30. http://dx.doi.org/10.26806/fd.v10i1.250.
Анотація:
In his (several times reprinted) article How Wide Is the Gap Between Facts and Values? N. Rescher aspires to clarify the long-lasting discussion on the ‘is-ought’ (‘fact-value’) gap by providing a framework in which the related arguments can be perspicuously articulated. He then argues that even if the logical gap may bereal, the transition from factual premises to value conclusions is smoothly mediated by trivially true value statements. We scrutinize Rescher’s argumentation and show defects in the presented lines of reasoning. In particular, we argue that his tripartite division of statements (factual, evaluative, hybrid), as well as his emphasis on the role of evaluative truisms, introduce serious problems that escape Rescher’s attention. We thus conclude that he failed to achieve his goals.
17
Kolář, Petr, and Vladimír Svoboda. "How Rescher Failed to Fill the Fact/Value Gap." FILOSOFIE DNES 10, no. 1 (April 13, 2019): 4–30. http://dx.doi.org/10.26806/fd.v10i1.417.
Анотація:
In his (several times reprinted) article How Wide Is the Gap Between Facts and Values? N. Rescher aspires to clarify the long-lasting discussion on the ‘is-ought’ (‘fact-value’) gap by providing a framework in which the related arguments can be perspicuously articulated. He then argues that even if the logical gap may bereal, the transition from factual premises to value conclusions is smoothly mediated by trivially true value statements. We scrutinize Rescher’s argumentation and show defects in the presented lines of reasoning. In particular, we argue that his tripartite division of statements (factual, evaluative, hybrid), as well as his emphasis on the role of evaluative truisms, introduce serious problems that escape Rescher’s attention. We thus conclude that he failed to achieve his goals.
18
Meyer, Barbara E., Elena Gaertner, and Christian Elting. "Closing the Gap: The Tripartite Structure of Sustainability as a Tool for Sustainable Education—A Systematic Literature Review." Sustainability 16, no. 9 (April 26, 2024): 3622. http://dx.doi.org/10.3390/su16093622.
Анотація:
(1) In an era where sustainable behavior is increasingly crucial, understanding the discrepancy between individuals’ sustainability-oriented values and their actual behaviors, known as the inner–outer gap, is vital. This systematic literature review explores the potential of the Tripartite Structure of Sustainability (TSS) framework to address this gap within the context of sustainable education. By reviewing the literature from the APA, ERIC, and Web of Science databases, searched on 25 October 2020, the factors influencing sustainable actions were systematically examined. Articles had to be relevant to the topic (sustainability, morality in relation to sustainability, or morality in general) and report on empirically validated factors that have an impact on the inner-outer gap. (2) By employing a qualitative content analysis approach, 56 articles over a 15-year period were analyzed, identifying 83 factors that either bridged or reinforced the inner–outer gap. These factors were categorized within the TSS framework, which segments sustainability-oriented actions into individual, social, and self-transcendent domains, alongside their activation points: stable, situational, or automated. (3) The analysis revealed that self-focused factors often reinforce the gap, suggesting they are a hindrance to sustainable behavior. Conversely, self-transcendent factors consistently bridged the gap, promoting sustainability. Social factors showed variable impacts based on the ethical and sustainable context they were placed in, suggesting that the social environment’s orientation significantly influences sustainable behavior. (4) This study concludes that the TSS framework offers a promising approach to advancing education for sustainable development (ESD) and contributes insights into how to promote the required paradigm shift towards holistic and interconnected perspectives.
19
Holdier, A. G. "Kierkegaard’s Three Spheres and Cinematic Fairy Tale Pedagogy in Frozen, Moana, and Tangled." Journal of Religion and Popular Culture 33, no. 2 (July 1, 2021): 105–19. http://dx.doi.org/10.3138/jrpc.2018-0027.
Анотація:
Although Disney films are sometimes denigrated as popular or “low” art forms, this article argues that they often engage deeply with, and thereby communicate, significant moral truths. The capitalistic enterprise of contemporary modern cinema demands that cinematic moral pedagogy be sublimated into non-partisan forms, often by substituting secular proxies for otherwise divine or spiritual components. By adapting Søren Kierkegaard’s tripartite existential anthropology of the self, I analyze the subjective experiences of the protagonists in three recent animated fairy tales—Disney’s Frozen, Moana, and Tangled—to demonstrate how these princess movies bridge the imaginative gap between the mundane and the divine.
20
Chen, Fang Yuan, Ting Jyun Jheng, and Daniel Liu. "The Experience and Performance of Female Airline Pilots in Taiwan - A Tripartite Assessment." International Business Research 15, no. 12 (November 11, 2022): 27. http://dx.doi.org/10.5539/ibr.v15n12p27.
Анотація:
The pilot profession remains one of the most notable gender-imbalanced occupations in the aviation industry, with women making up a far lower percentage than men. Nevertheless, the experiences and challenges faced by female pilots in the workplace is worth exploring. Previous studies have mostly approached these issues from the perspective of male and/or female pilots, ignoring the opinions of managers responsible for flight operations. This study fills this research gap by adding flight operations managers' assessments of female pilots to explore the topic from a broader and comprehensive tripartite perspective. In addition, gender issues in the cockpit and the impact of female pilot participation are also discussed. The research is conducted through in-depth interviews covering flight operations managers, male pilots, and female pilots in three different Taiwanese airlines. The findings of this study reveal that the performance of female pilots is generally affirmed by flight operations managers and male pilots, and the participation of female pilots produces some chemical effects on the culture of the cockpit, which also contributes to crew resource management and flight safety. Contrary to previous research findings, the female pilots interviewed in this study do not feel that they are being challenged or abused in relation to their gender. Finally, several recommendations are given to the airlines to implement CRM training programs and recruit female pilots.
21
Olmstead, Annie J., Jimin Lee, and Navin Viswanathan. "The Role of the Speaker, the Listener, and Their Joint Contributions During Communicative Interactions: A Tripartite View of Intelligibility in Individuals With Dysarthria." Journal of Speech, Language, and Hearing Research 63, no. 4 (April 27, 2020): 1106–14. http://dx.doi.org/10.1044/2020_jslhr-19-00233.
Анотація:
Purpose We present a tripartite view of intelligibility in which the contributions of both the speaker and listener, as well as their joint effort during interaction, are considered. While considerable research has examined communicative interactions in situ, there is a critical gap in current knowledge on how speech intelligibility unfolds during such interactions. Here, we argue that research examining speech intelligibility in communicative interactions may provide important groundwork for advancement in clinical interventions for individuals with dysarthria. Method First, we describe the view and argue for its consideration as a powerful way of thinking about speech intelligibility. We then briefly situate the view in the relevant literature on speech intelligibility and existing theoretical frameworks. We then identify suitable methodological paradigms for studying joint contributions to intelligibility and, lastly, discuss the clinical application and potential impact of this tripartite view. Conclusions Speech communication occurs through interaction; however, in the laboratory and clinic, emphasis is usually placed on individual speakers and listeners. We have proposed that it is critical to consider how the joint contributions of speakers and listeners affect speech intelligibility in communicative interaction. This conceptualization is well aligned with the International Classification of Functioning, Disability and Health, and the findings from such an approach will allow us to better understand how to maximize available resources to enhance speech intelligibility.
22
Franco, Diana. "Trauma Without Borders: The Necessity for School-Based Interventions in Treating Unaccompanied Refugee Minors." Child and Adolescent Social Work Journal 35, no. 6 (May 29, 2018): 551–65. http://dx.doi.org/10.1007/s10560-018-0552-6.
Анотація:
AbstractThis article explores migration trauma among Mexican and Central American unaccompanied refugee minors (URM) with the purpose of developing an understanding of migration as a tripartite process consisting of: pre-migration exposure to traumatic stressors, in-journey stressors, and post-migration stressors. The migration experience of these youth may be subjectively different depending on a wide range of factors. The complexities of migration are explored as a traumatic, tripartite process. These three salient components of migration may act as precursors, often resulting in psychological sequelae such as: post-traumatic stress disorder (PTSD), anxiety, and depression. Of all migrant groups, URM are more likely to develop psychiatric symptoms. Trauma-Focused Cognitive Behavioral Therapy (TF-CBT), Cognitive Behavioral Intervention for Trauma in Schools (CBITS), and Mental Health for Immigrants Program (MHIP) are among the most effective interventions in the treatment of PTSD, anxiety, and depression in refugee minors. Social workers in schools are in unique positions to provide mental health services to URM. A case example illustrating a cultural adaptation of TF-CBT in an urban public high school is included. Clinical implications of culturally responsive and trauma-informed treatment of URM in schools will be discussed. Additionally, this article will emphasize the importance of bridging the gap between research and culturally responsive, trauma-informed interventions for URM in schools.
23
Ettah, Emmanuel, Michael E. Ishaje, Kseniia Minakova, Esther Offiong Asuquo, and Stephen U. Odey. "EVALUATION OF ENERGY BAND STRUCTURE OF HALF-HEUSLER ALLOY LiZnX (X = As, P, and Sb) USING FIRST PRINCIPLE CALCULATION." Bulletin of the National Technical University «KhPI» Series: New solutions in modern technologies, no. 3(17) (October 5, 2023): 11–16. http://dx.doi.org/10.20998/2413-4295.2023.03.02.
Анотація:
The evaluation of energy band structure plays a vital role in understanding the electronic properties of materials. This research, we investigate the energy band structure of Half-Heusler alloys LiZn(X = As, P, and Sb) using a first principle approach based on Density Functional Theory (DFT). These alloys are of particular interest due to their potential applications in thermoelectric and spintronics devices. The corresponding Density of States (DOS) for the tripartite compounds LiZnX (X=As, P, and Sb) have been calculated and the contributions of the Li, Zn, As, P and Sb orbital to the Density of States at ambient pressure. This also confirmed that LiZnX (X=As, P, and Sb) is a semi-conductor with a narrow band-gap between the occupied and unoccupied regions around the Fermi level. The orbitals Li -1s, As-4p, As- 4s, Zn-3d has the highest contributions. The dominant of the orbitals P-1s and P-2p before the Fermi- level and Zn-2p after the Fermi-level are observed. We observed the dominant of the orbitals Sb-1s, Sb-3d, Li-1s, Li-2s, Zn-3d shows weak hybridization and low contribution. This features indicates that the covalent bond between these two atoms is weak, and could be responsible for the mechanical instability observed in the calculation. Meanwhile the band structure calculated and presented has narrow band-gab of 0.625. 0.937 and 0.313 respectively for the tripartite compound LiZnX(X=As, P, and Sb) and its a direct band-gap semiconductor. The obtained energy band structures provide valuable information about the electronic properties of LiZn (X = As, P, and Sb) alloys. The presence of band gaps is crucial for thermoelectric applications, as it indicates the presence of regions where electrons and holes are confined, enabling efficient charge transport.
24
Islam, Faridul, and Saleheen Khan. "The long run impact of immigration on labor market in an advanced economy." International Journal of Social Economics 42, no. 4 (April 13, 2015): 356–67. http://dx.doi.org/10.1108/ijse-12-2013-0291.
Анотація:
Purpose – The purpose of this paper is to examine the dynamic relationship among immigration rate, GDP per capita, and and real wage rates in the USA. Design/methodology/approach – The paper implements the Johansen-Juselius (1990, 1992) cointegration technique to test for a long-run relationship; and for short-run dynamics the authors apply Granger causality tests under the vector error-correction model. Findings – The results show that the long-run causality runs from GDP per capita to immigration, not vice versa. Growing economy attracts immigrants. The authors also find that immigration flow depresses average weekly earnings of the natives in the long-run. Originality/value – The authors are not aware of any study on the USA addressing the impact of immigrants on labor market using a tripartite approach by explicitly incorporating economic growth. It is therefore important to pursue a theoretically justified empirical model in search of a relation to resolve on apparent immigration debate.
25
De Castro Pericacho, Carlos, Elena Gadea Montesinos, and Miguel Ángel Sánchez García. "Estandarizadores. La nueva burocracia privada que controla la calidad y la seguridad alimentaria en las cadenas globales agrícolas." Revista Española de Sociología 30, no. 1 (January 21, 2021): a16. http://dx.doi.org/10.22325/fes/res.2021.16.
Анотація:
La producción global agroalimentaria se encuentra estructurada en cadenas sobre las que los grandes grupos de distribución (marcas, supermercados) ejercen un estrecho control. En las últimas décadas, los estándares privados de calidad y seguridad se han convertido en una de las formas más importantes de gobernanza de estas cadenas. Nuestro objetivo es analizar la estructura institucional desde la que se diseñan estos requisitos de calidad y seguridad y los procedimientos de evaluación. La hipótesis que planteamos es que el poder de los grandes distribuidores aumenta gracias al control de esa estructura institucional, que denominamos régimen tripartito de los estándares (Loconto y Busch, 2010). Para dar cuenta de ello analizaremos los estándares privados mercantiles que predominan en una de las regiones exportadoras de frutas y verduras más importantes de Europa, la Región de Murcia. Mostraremos que el régimen tripartito de los estándares privados de los grandes distribuidores (Global GAP, IFS, BRC) presenta tres característicasprincipales: la hibridación de lo público y lo privado en el ámbito de la regulación; la burocratización por medio de profesionales expertos, conocimientos especializados y procedimientos procedentes de actores privados; y el control de los grandes distribuidores sobre todo el proceso de estandarización.
26
Marima, Rahaba, Rodney Hull, Georgios Lolas, Konstantinos N. Syrigos, Minah Kgoebane-Maseko, Andreas Martin Kaufmann, and Zodwa Dlamini. "The Catastrophic HPV/HIV Dual Viral Oncogenomics in Concert with Dysregulated Alternative Splicing in Cervical Cancer." International Journal of Molecular Sciences 22, no. 18 (September 18, 2021): 10115. http://dx.doi.org/10.3390/ijms221810115.
Анотація:
Cervical cancer is a public health problem and has devastating effects in low-to-middle-income countries (LTMICs) such as the sub-Saharan African (SSA) countries. Infection by the human papillomavirus (HPV) is the main cause of cervical cancer. HIV positive women have higher HPV prevalence and cervical cancer incidence than their HIV negative counterparts do. Concurrent HPV/HIV infection is catastrophic, particularly to African women due to the high prevalence of HIV infections. Although various studies show a relationship between HPV, HIV and cervical cancer, there is still a gap in the knowledge concerning the precise nature of this tripartite association. Firstly, most studies show the relationship between HPV and cervical cancer at genomic and epigenetic levels, while the transcriptomic landscape of this relationship remains to be elucidated. Even though many studies have shown HPV/HIV dual viral pathogenesis, the dual molecular oncoviral effects on the development of cervical cancer remains largely uncertain. Furthermore, the effect of highly active antiretroviral therapy (HAART) on the cellular splicing machinery is unclear. Emerging evidence indicates the vital role played by host splicing events in both HPV and HIV infection in the development and progression to cervical cancer. Therefore, decoding the transcriptome landscape of this tripartite relationship holds promising therapeutic potential. This review will focus on the link between cellular splicing machinery, HPV, HIV infection and the aberrant alternative splicing events that take place in HIV/HPV-associated cervical cancer. Finally, we will investigate how these aberrant splicing events can be targeted for the development of new therapeutic strategies against HPV/HIV-associated cervical cancer.
27
Kim, Yoo Sung, Juwon Choi, and Bo-Eun Yoon. "Neuron-Glia Interactions in Neurodevelopmental Disorders." Cells 9, no. 10 (September 27, 2020): 2176. http://dx.doi.org/10.3390/cells9102176.
Анотація:
Recent studies have revealed synaptic dysfunction to be a hallmark of various psychiatric diseases, and that glial cells participate in synapse formation, development, and plasticity. Glial cells contribute to neuroinflammation and synaptic homeostasis, the latter being essential for maintaining the physiological function of the central nervous system (CNS). In particular, glial cells undergo gliotransmission and regulate neuronal activity in tripartite synapses via ion channels (gap junction hemichannel, volume regulated anion channel, and bestrophin-1), receptors (for neurotransmitters and cytokines), or transporters (GLT-1, GLAST, and GATs) that are expressed on glial cell membranes. In this review, we propose that dysfunction in neuron-glia interactions may contribute to the pathogenesis of neurodevelopmental disorders. Understanding the mechanisms of neuron-glia interaction for synapse formation and maturation will contribute to the development of novel therapeutic targets of neurodevelopmental disorders.
28
Fadzli Ahmad Tajuddin, Ahmad, Anis Yusal Yusoff, and Mohd Rezaidi Ishak. "Enhancing Integrity to Strengthen Organizational Performance of Local Authorities in Malaysia." International Journal of Engineering & Technology 7, no. 2.29 (May 22, 2018): 326. http://dx.doi.org/10.14419/ijet.v7i2.29.13647.
Анотація:
The main theme of this paper encompasses on the operationalization of the notion of integrity focusing on the performance of local authorities (LAs) in Malaysia. Specifically, the study applies the Community Integrity Building (CIB) concept which highlighted on the significant contribution of tripartite parties in gearing for better organizational performance namely community monitors, contractors and LAs. Furthermore, CIB creates an enhanced feedback loop of continuous improvement and vigilance, creating better community over time. The study also pioneered on measuring of fix rate methodology and integrity approach objectively; thus bridging the gap between conceptualization and operationalization spectrum. Finally, the paper concludes by enlightening and giving some insights on the potentials of CIB approach and changing the typical art of doing things in public service especially with regards to integrity agenda vis-‘a-vis building stronger local community.
29
van Riper, Carena J., Clinton Lum, Gerard T. Kyle, Kenneth E. Wallen, James Absher, and Adam C. Landon. "Values, Motivations, and Intentions to Engage in Proenvironmental Behavior." Environment and Behavior 52, no. 4 (November 1, 2018): 437–62. http://dx.doi.org/10.1177/0013916518807963.
Анотація:
Knowledge of the relationships among psychological constructs such as values and motivations that influence proenvironmental behavior provides public land management agencies with guidance on how to minimize stakeholder impacts on the environment. A rich body of research has demonstrated that values form a tripartite structure underlying environmental concern, encompassing biospheric, egoistic, and altruistic values; however, recent work has suggested hedonic values are also an instrumental basis for environmental concern. Few studies have tested this proposition. We contend that hedonic values are instrumental in explaining the psychological processes that gird individual decisions, particularly in nature-based settings where stakeholder decisions are compelled by leisure pursuits. Our results indicate that place-based motivations, particularly escape from the pressures of everyday life, can help close the prominent value–action gap and explain why outdoor recreationists engage in minimum-impact activities specified in the U.S. Leave No Trace educational outreach program.
30
Adeosun, Oluyemi Theophilus, Simeon Ernest Odior, Ibrahim Ayodele Shittu, and Waliu Mulero Adegbite. "Industrial Sector Performance, Human Capital Development and Economic Growth in Nigeria." International Research Journal of Business Studies 16, no. 2 (December 15, 2023): 163–77. http://dx.doi.org/10.21632/irjbs.16.2.163-177.
Анотація:
This study examines the relationship between industrial sector performance (ISP), human capital development (HCD), and economic growth (EG) in Nigeria. Data used in this study was obtained from World Development Indicators (WDI) and Penn World from 1981 to 2020 on GDP/capita, human capital index, and industrial output. The augmented Dickey-Fuller (ADF) test was applied to obtain the stationarity conditions of the variables before performing the autoregressive distributed lag test to determine the short- and long-run relationships among the variables. The study reveals no long-run relationship among the variables; however, a negligible impact on human capital and industrial output in the short term exists, highlighting the need for government intervention. This is the first study to examine the tripartite relationship between industrial sector performance, human capital development, and economic growth in the Nigerian context, providing insight into the dynamics of the relationships in developing countries.
31
Peng, Xue, Fulin Wang, Jiquan Wang, and Chang Qian. "Research on Food Safety Control Based on Evolutionary Game Method from the Perspective of the Food Supply Chain." Sustainability 14, no. 13 (July 3, 2022): 8122. http://dx.doi.org/10.3390/su14138122.
Анотація:
Food safety is related to people’s health and the sustainable development of the food supply chain, so it is of crucial practical significance to study food supply chain quality management. This paper employs the evolutionary game method to develop a tripartite evolutionary game model that reflects the interaction of interests among food raw material suppliers, food manufacturers, and consumers. It identifies the key factors that influence the decision-making of each game participant and attempts to use these factors to guide the behavior of the food supply chain members scientifically. The study results demonstrate that sustainable management of food supply chains can be improved by strengthening the intensity of government supervision, narrowing the production cost gap between high-quality and poor-quality food raw materials, improving the inspection ability of the food manufacturer, increasing complaint incentives of consumers, etc. Finally, this paper puts forward suggestions and countermeasures for the government to improve the supervision mechanism of food safety, thereby achieving sustainable management of the food supply chain.
32
Ulf, Christoph. "From theory to empirical research." Archaeological Dialogues 17, no. 1 (May 4, 2010): 96–100. http://dx.doi.org/10.1017/s1380203810000115.
Анотація:
Sofia Voutsaki has developed a very stimulating line of thought in her paper. In my view, one of the laudable traits of this paper is that it is characterized by the same tripartite structure which actually lies behind each scientific argument. Consciously or not, all of us start with a theory or a set of assumptions; we then proceed to methods in order to achieve our goal, i.e. to arrive at transparent interpretations of the past through empirical analysis. The analysis of empirical data is the end of the process, not its starting point, even if many people think it would be the beginning of our daily research work. The claim that the use of theory is unavoidable is often denied. Sofia Voutsaki's goal, as I understand it, is to make an attempt to narrow the gap between, on the one hand, mainly theory-driven research and, on the other, empirical analysis which is thought to be free from the unnecessary ‘burden’ of theory.
33
Aden Dirir, Sadik. "The Effect of Institutional Quality on Economic Growth: Evidence from Tripartite Approaches in the Context of War-Torn Countries." Organizations and Markets in Emerging Economies 14, no. 3 (December 21, 2023): 508–35. http://dx.doi.org/10.15388/omee.2023.14.3.
Анотація:
This paper is undertaken to investigate the role of institutional quality in proportion to the economic growth of war-torn countries during the last 20 years. Within this framework, the paper employed three different models to investigate the link that exists between good governance indicators and economic growth. According to the results, the long-run PMG model indicated that political stability and regulatory quality increase war-torn countries’ economic development. Contrarily, it has been demonstrated that the situation of law and corruption in these countries reduces economic growth. Neither the long-term nor short-term estimations from the MG and DFE models showed any noteworthy results. Next, the FMOLS and DOLS revealed that political stability, voice and accountability have a favorable effect on the economic development of these nations. On the other hand, these nations’ regulatory standards had a very detrimental impact on economic growth. Lastly, the fixed-effects model showed that a 1% improvement in these nations’ political stability will result in a 4.5% increase in GDP. This research will aid managers, academics, and policymakers in determining the course of actions needed in their areas of specialization or nations of interest to ensure economic growth and put in place an effective institutional framework through enforced supervision.
34
Mohapatra, Subhalaxmi. "Economic growth, public expenditure on health and IMR in India." International Journal of Social Economics 44, no. 12 (December 4, 2017): 2002–18. http://dx.doi.org/10.1108/ijse-05-2015-0121.
Анотація:
Purpose The purpose of this paper is to employ a two-step approach to investigate the bi-directional causal linkage between: economic growth (measured by GDP) and public expenditure on health; public expenditure on health and infant mortality rate (IMR); and economic growth and IMR in the Indian context. Design/methodology/approach The present study uses econometric analysis, namely, panel cointegration and Granger causality on 20-year panel data on 16 major Indian states to investigate the causality. Findings The results suggest GDP to Granger cause public expenditure on health both in the short run and in the long run, but public expenditure on health to Granger cause GDP only in the long run. Further, public expenditure on health and economic growth were found to Granger cause IMR in the long run. However, the reverse linkage from IMR to public expenditure on health and/or economic growth was not significant. Research limitations/implications The present study provides support to the existing literature on the effects of economic growth on health expenditure and health outcomes but also raises a question on the time required to realize the same. Practical implications The findings have implications for policy makers on the time frame and application of health expenditure to achieve better results. Originality/value The present study is one of the first to test the tripartite linkage between economic growth, public health expenditure and health outcomes at a state-level analysis.
35
Wang, Xuwei, Kaiwen Ji, and Tongping Xie. "AI Carbon Footprint Management with Multi-Agent Participation: A Tripartite Evolutionary Game Analysis Based on a Case in China." Sustainability 15, no. 11 (June 2, 2023): 9013. http://dx.doi.org/10.3390/su15119013.
Анотація:
AI is playing an important role in promoting sustainable development, but the carbon footprint caused by AI is scaling quickly and may partly offset the effort to reduce carbon emissions. However, recommendations for limiting the AI carbon footprint are lacking. In order to address this gap in the literature, this paper first constructs a tripartite evolutionary game model by taking governments, AI industry alliances, and consumers into consideration, and then exploring the impacts of key factors on these three players’ strategy selection based on the case of smart air conditioner consumption in China. The results show that the behavior of governments has an important influence on the behavior of AI industry alliances and consumers. The ideal consequence is that governments adopt an unregulated strategy, AI industry alliances adopt a green development strategy, and consumers adopt a green purchase strategy. Regulation by governments is indispensable for limiting the AI carbon footprint during an early stage but becomes dispensable when the system reaches an optimal state. Although a tendency toward green consumption, image benefit, regulatory cost, carbon price, and the subsidies given to consumers and AI industry alliances can largely influence the strategy selection of governments, governments are most sensitive to carbon prices and the subsidies given to consumers. AI industry alliances are not sensitive to subsidies, reputation improvement, and reputation loss but are most sensitive to carbon prices. Consumers are most sensitive to green consumption tendencies, self-satisfaction, and utility but are not sensitive to subsidies.
36
Jin, Luosong, Chang He, Xiangyang Wang, Wei Wang, and Panting Zhao. "Study on the Compliance Management of the Electricity Market in China Based on the Evolutionary Game Theory." Complexity 2021 (March 12, 2021): 1–22. http://dx.doi.org/10.1155/2021/5532763.
Анотація:
China’s new round of power system reform has further released market vitality, making the power market more open and diversified. Meanwhile, China’s power market faces various risks and challenges incurred by this new reform, which further highlights the importance of the compliance management. However, the study on effective compliance management of China’s power market is missing, and the way to achieving effective compliance management is still unanswered. This paper tries to fill the research gap using the evolutionary game theory. We constructed a tripartite game model to analyze the strategic choices and influencing factors of power generators, compliance departments of the trading centre, and government regulatory agencies. Furthermore, simulation analysis was conducted based on evolutionary stable strategies. The results show that effective compliance management can be achieved without government supervision if the market mechanism is properly designed. In addition, the costs and profits of market participants and regulators are important factors influencing the effectiveness of compliance management. Our findings may arouse inspiration for the policy makers to construct an effective compliance management system.
37
Bak, Ozlem. "Creating a Bridge between Industry and Higher Education through an Operations Consultancy Module." Industry and Higher Education 25, no. 3 (June 2011): 205–11. http://dx.doi.org/10.5367/ihe.2011.0037.
Анотація:
This paper explores the challenges and use of a postgraduate consultancy module in teaching operations management. The consultancy module extends the traditional learning boundaries between students and lecturers to a tripartite grouping of students, university and industry. The interaction within this group may be influenced by students' skills and their level of understanding and the needs of the particular industry. The consultancy module and the students' solutions are thus influenced by cooperation and teamwork and the autonomy, skills and training of the students as part of the application of operations management theory. The paper also presents an evaluation of the perceived gap between teaching operations management theory in higher education and its application in practice (in industry). It is argued that providing an operations consultancy module as a strategic teaching tool encourages postgraduate students, lecturers and industry to build a bridge between the relatively distinct worlds of academia and industry. The research is intended to help operations management educators to identify and deal with the challenges of setting up a consultancy module in higher education.
38
Ennals, Richard, Peter Totterdill, and Campbell Ford. "The Work Research Foundation." Concepts and Transformation 6, no. 3 (December 31, 2001): 259–73. http://dx.doi.org/10.1075/cat.6.3.04enn.
Анотація:
Despite its early contributions to the development of working life research the UK has lagged behind much of the rest of Northern Europe in establishing a coherent approach to the modernization of work organization. The removal of tripartite structures by the Thatcher and Major governments and their decision to opt out of significant areas of European employment policy left the UK ill-prepared to respond to emerging economic or policy challenges in Europe. Evidence of an increasing gap between leading-edge practice and common practice in UK workplaces has emerged forcibly as a key issue for future productivity and employment. The UK Work Organization Network (UK WON) was first established in 1996 as a coalition between researchers, business support organizations and social partners, slowly building a portfolio of projects designed to support workplace innovation. More recently the creation of the Work Research Foundation, a partnership-based company with responsibility for managing the activities of the Network, firmly establishes UK WON as a significant vehicle for social dialogue and organizational change.
39
Okada, Motohiro, Tomoka Oka, Misaki Nakamoto, Kouji Fukuyama, and Takashi Shiroyama. "Astroglial Connexin43 as a Potential Target for a Mood Stabiliser." International Journal of Molecular Sciences 22, no. 1 (December 30, 2020): 339. http://dx.doi.org/10.3390/ijms22010339.
Анотація:
Mood disorders remain a major public health concern worldwide. Monoaminergic hypotheses of pathophysiology of bipolar and major depressive disorders have led to the development of monoamine transporter-inhibiting antidepressants for the treatment of major depression and have contributed to the expanded indications of atypical antipsychotics for the treatment of bipolar disorders. In spite of psychopharmacological progress, current pharmacotherapy according to the monoaminergic hypothesis alone is insufficient to improve or prevent mood disorders. Recent approval of esketamine for treatment of treatment-resistant depression has attracted attention in psychopharmacology as a glutamatergic hypothesis of the pathophysiology of mood disorders. On the other hand, in the last decade, accumulated findings regarding the pathomechanisms of mood disorders emphasised that functional abnormalities of tripartite synaptic transmission play important roles in the pathophysiology of mood disorders. At first glance, the enhancement of astroglial connexin seems to contribute to antidepressant and mood-stabilising effects, but in reality, antidepressive and mood-stabilising actions are mediated by more complicated interactions associated with the astroglial gap junction and hemichannel. Indeed, several depressive mood-inducing stress stimulations suppress connexin43 expression and astroglial gap junction function, but enhance astroglial hemichannel activity. On the other hand, monoamine transporter-inhibiting antidepressants suppress astroglial hemichannel activity and enhance astroglial gap junction function, whereas several non-antidepressant mood stabilisers activate astroglial hemichannel activity. Based on preclinical findings, in this review, we summarise the effects of antidepressants, mood-stabilising antipsychotics, and anticonvulsants on astroglial connexin, and then, to establish a novel strategy for treatment of mood disorders, we reveal the current progress in psychopharmacology, changing the question from “what has been revealed?” to “what should be clarified?”.
40
Kasih, Desak Putu Dewi, Nyoman Satyayuda Dananjaya, Kadek Agus Sudiarawan, and I. Putu Bimbisara Wimuna Raksita. "Constructive Termination of Employment by Indonesia Companies: A Comparative Study." Substantive Justice International Journal of Law 4, no. 2 (September 29, 2021): 97. http://dx.doi.org/10.33096/substantivejustice.v4i2.143.
Анотація:
This research aims to identify the regulation and dispute resolution regarding constructive termination of employment by a company in the perspective of Indonesian Labor Law. This research also compares the constructive termination of employment by companies based on International Law and Japan Labor Law. This is normative legal research with a statutory approach, conceptual approach, and also comparative law approach. The result shows that the constructive termination of employment has not been specifically regulated in the Indonesian Labor Law system, hence it becomes an exploitation gap that is used by companies to be able to terminate employment relations without protecting the worker’s right. The dispute resolution mechanism in terms of constructive termination of employments still refers to the provisions of the Industrial Relations Dispute Settlement Law, namely through bipartite negotiations, tripartite, and industrial relations courts. The constructive termination of employment has been substantively regulated in the ILO Convention C-158 concerning the Termination of Employment, which regulated in Article 4 to Article 6. Further in Japan, the constructive termination of employment is regulated in the Japan Labor Union Act, Act Number 174 of 1949 in Article 7 paragraph (i), (iii), and (iv) with its national enforcement.
41
ALCALDE, LEANDRO, FLORENCIA VERA CANDIOTI, FRANCISCO KOLENC, CLAUDIO BORTEIRO, and DIEGO BALDO. "Cranial anatomy of tadpoles of five species of Scinax (Hylidae, Hylinae)." Zootaxa 2787, no. 1 (March 10, 2011): 19. http://dx.doi.org/10.11646/zootaxa.2787.1.2.
Анотація:
We studied the oral apparatus, buccal cavity and musculoskeletal features in tadpoles of five species of the genus Scinax (S. acuminatus, S. uruguayus, S. aff. pinima, S. aromothyella, and S. berthae). Observed variation is mainly related to intrageneric grouping. Scinax acuminatus (S. ruber clade, sister taxon of S. rostratus group) has a distinctive combination of a mental gap in the margin of oral papillae, straight labial teeth with few or absent cusps, processus muscularis acute and posteriorly directed, and m. subarcualis rectus I with two slips. Scinax uruguayus and S. aff. pinima (S. uruguayus group) have keratinized sheets ventrolateral to the lower jaw sheath, well-developed infralabial and lateral ridge papillae, robust jaw cartilages, cornua trabeculae with short and widely divergent free portions, processus articularis short and wide, processus muscularis thin and directed anteriorly. Scinax aromothyella and S. berthae (S. catharinae group) have poorly developed, non-colored spurs behind the lower jaw sheath, long and thin processus articularis, wide and rounded processus muscularis, and tripartite cartilago suprarostralis. Anatomical features described are congruent with current phylogenetic arrangements based on molecular, chromosomal, and morphological data, and provide a source of information that can be useful to solve interspecific relationships within Scinax.
42
Guo, Shengyu, Pan Zhang, and Jianying Yang. "SYSTEM DYNAMICS MODEL BASED ON EVOLUTIONARY GAME THEORY FOR QUALITY SUPERVISION AMONG CONSTRUCTION STAKEHOLDERS." Journal of Civil Engineering and Management 24, no. 4 (June 29, 2018): 318–30. http://dx.doi.org/10.3846/jcem.2018.3068.
Анотація:
To address the gap, that is, few studies have explored the influence of the participants’ interactions with one another during construction quality supervision, this paper proposes a system dynamics model based on evolutionary game theory to describe the complex and dynamic interactions among tripartite stakeholders in China, including the project owner (PO), construction supervising engineer (CSE), and construction contractor (CC). First, the replicated dynamic equation set is established in terms of expense targets. Second, the equilibrium solutions of the equation set are obtained to test strategy options. The trends of system fluctuations caused by penalty and reward changes are also analyzed. Finally, the stability of the proposed model is improved by integrating a dynamic penalty–reward scenario into the evolutionary strategy of the PO. Simulation results show that: 1) the evolutionary stable strategy does not exist in initial interactions, 2) the degrees of penalty and reward considerably affect the CC’s rate variable, and 3) the dynamic penalty–reward scenario could effectively improve the stability of the proposed model. The unsteadiness of the quality supervision system and the stability control scenario could help in understanding the impact of interactions among stakeholders and provide suggestions for optimizing quality supervision procedures.
43
Schoeler, Gregor. "The “National Amnesia” in the Traditional History of Iran." Der Islam 97, no. 2 (October 7, 2020): 500–532. http://dx.doi.org/10.1515/islam-2020-0031.
Анотація:
AbstractIt is well known that the pre-Islamic “national history” of Iran (i. e., the indigenous secular historical tradition, transmitted orally over many centuries) knows nothing at all, or as good as nothing, about the dynasties and empires of the Medes, Achaemenids, Seleucids, and Parthians (ca. 700 BCE–226 CE). It is first with the Sasanians (226‒651 CE) that Iran’s “national history” evinces more detailed knowledge. Instead of reports on the historical Medes and Achaemenid dynasties, accounts of mythical and legendary dynasties, the Pīšdādians and Kayānians, are found.In this essay, an attempt will be made to explain this “gap” in the pre-Islamic historical tradition, this “strange historical (or national) amnesiaˮ (Ehsan Yarshater) in the cultural memory of the Iranians, with the help of a theory on the structure and modality of oral tradition, based on field research, by the Belgian historian and anthropologist Jan Vansina. The structure in question concerns a tripartite perception of the past: a wealth of information about antiquity (traditions of origin or creation and reports on culture heroes) – plenty of information, too, on the recent and most recent times – and lying between them, a “gap” in the accounts. Vansina described this phenomenon as the “hourglass effect.” This is exactly the narrative structure of Iranian national history; it is evident that the Achaemenids and the other pre-Christian dynasties fall into the “gap” described by Vansina.The same phenomenon can also be detected on the level of Sasanian history. We find there a plethora of information on the founder of the dynasty, Ardašīr (reigned 226‒241 CE); meanwhile, very few details are known of the kings following Ardašīr, and it is only as of Kavād I (reigned 488‒496 and 499‒531 CE) that we have outstanding historical information.
44
Lim, Jaejoo, Jim R. Wollscheid, and Ramakrishna Ayyagari. "Examining the effectiveness of compensatory adaptation from a consumer's perspective in evaluating products online." American Journal of Business 35, no. 2 (April 6, 2020): 61–81. http://dx.doi.org/10.1108/ajb-09-2019-0070.
Анотація:
PurposeConsumers often encounter issues of perceived ambiguity and performance risk when attempting to evaluate experience goods being offered online. Sellers try to alleviate this knowledge gap often seen in a medium of low naturalness by engaging in effective compensatory adaptation. This research theoretically looks into three primary aspects of compensatory adaption and their potential in securing communication of high-quality information between the online seller and consumer.Design/methodology/approachUtilizing survey data and structural equation modeling, this study tests the effectiveness of different aspects of compensatory adaption to alleviate the knowledge gap in a medium of low naturalness.FindingsDrawing on media naturalness theory and the tripartite model of attitude, this paper identifies three theoretical components that significantly affect the effectiveness of compensatory adaption. They are information retrieval capability from the cognitive/logical aspect, information richness from the affective/audiovisual aspect and interactivity from the behavioral aspect. The effectiveness of compensatory adaptation proves to have a positive impact on perceived information quality.Originality/valueTo the best of our knowledge, this is the first paper in the information systems literature to examine the compensatory adaptation tools for effective transfer of information. This study contributes to the academics by providing three handles to improve effectiveness of compensatory adaptation toward information quality. We focus on three compensatory adaptation tools in cognitive/logical, affective/audiovisual and behavioral aspects, and this compensation perspective leads to three practical factors that affect effective transfer of information between online sellers and consumers. The result of this study complements the nomological network of the enablers and impediments of e-commerce.
45
Lebedeva, A. V., K. E. Maltseva, R. A. Sokolov, N. V. Barabash, T. A. Levanova, and A. V. Rozov. "Effects of optogenetic astroglia activation in modulation of synaptic transmission and rhythmogenesis of the hippocampus." Genes & Cells 18, no. 4 (December 15, 2023): 504–7. http://dx.doi.org/10.17816/gc623466.
Анотація:
In the field of neuronal signal transduction research, astroglia have become increasingly important. Astrocytes, or astroglial cells, are recognized as the third element in synaptic transmission regulation. This complex is conventionally known as a tripartite synapse. A multitude of mathematical models are being developed to investigate the function of astroglia in the tripartite synapse. Neurobiological experiments were carried out to confirm the dynamics observed in the previously proposed mean field activity mathematical model [1]. The experiments focused on evaluating the effect of optogenetic activation of astrocytes on synaptic transmission in C57BL/6 inbred mouse hippocampal slices. One month prior to the experiments, the AAV GFAP ChR2 eYFP virus was injected into the lateral ventricles of the brains of the experimental mice. This procedure was crucial for the expression of astrocyte-specific photosensitive channels, also known as channel rhodopsin. The patch-clamp method was used to conduct experiments on both the experimental mice and a control group of mice that did not receive virus injections. Spontaneous neuronal activity (local field potentials) and synaptic currents (GABA currents) were simultaneously recorded in the experiments. After activating astrocytes that expressed the photosensitive channel, an increase in GABAergic currents became evident during the transmission of synaptic signals between neurons. This suggests that astrocytes likely modulate synaptic transmission by releasing a gliotransmitter into the synaptic cleft. Thus, the study confirmed the presence of mean field activity patterns in a phenomenological model that describes the dynamics of a population of neurons. This model was developed based on the Tsodyks–Markram model and considers the primary attributes of neuron-glial interaction through a tripartite synapse. The model includes the short-term synaptic plasticity of the Tsodyks–Markram model and the astrocyte potential of synaptic transmission. The activation of astrocytes results in a diverse range of dynamic modes that describe various patterns of network activity under the mean field approach framework. Currently, multiple experimental hypotheses exist regarding astrocytes’ release of a gliotransfer into the synaptic cleft and its specific type. Alternatively, a complex cascade of sequential activation of glutamateergic and GABAergic receptors may occur. Additional experimental work is necessary to assess the pharmacological contribution of channels and transporters involved in modulating synaptic transmission during astrocyte optogenetic activation. The obtained results will refine the mathematical model of mean field neuronal activity to increase its biological plausibility. The methodology used involves identifying sparse nonlinear dynamical systems from data by solving for the system’s equations of motion [2]. These equations are reconstructed from noisy measurement data, allowing for a more accurate representation of the dynamic system. The only assumption regarding the arrangement of a dynamical system is that there exist only a handful of significant factors regulating the dynamics, leading to equations that are sparse within the realm of potential functions. Sparse regression is used to ascertain the minimum number of terms required in dynamic equations for precise data representation. This strategy enables the construction of mathematical models that are both as accurate as feasible, and maximally uncomplicated, eliminating the need for retraining. Notably, this method is suitable for parameterized systems and systems subjected to external influences or changes over time. The two approaches were used to forecast the dynamics of the mean field of a neural population. The accuracy of the forecast was subsequently evaluated.
46
Hervé, Vincent, Anaële Simon, Finaritra Randevoson, Guillaume Cailleau, Gabrielle Rajoelison, Herintsitohaina Razakamanarivo, Saskia Bindschedler, Eric Verrecchia, and Pilar Junier. "Functional Diversity of the Litter-Associated Fungi from an Oxalate-Carbonate Pathway Ecosystem in Madagascar." Microorganisms 9, no. 5 (May 1, 2021): 985. http://dx.doi.org/10.3390/microorganisms9050985.
Анотація:
The oxalate-carbonate pathway (OCP) is a biogeochemical process linking oxalate oxidation and carbonate precipitation. Currently, this pathway is described as a tripartite association involving oxalogenic plants, oxalogenic fungi, and oxalotrophic bacteria. While the OCP has recently received increasing interest given its potential for capturing carbon in soils, there are still many unknowns, especially regarding the taxonomic and functional diversity of the fungi involved in this pathway. To fill this gap, we described an active OCP site in Madagascar, under the influence of the oxalogenic tree Tamarindus indica, and isolated, identified, and characterized 50 fungal strains from the leaf litter. The fungal diversity encompassed three phyla, namely Mucoromycota, Ascomycota, and Basidiomycota, and 23 genera. Using various media, we further investigated their functional potential. Most of the fungal strains produced siderophores and presented proteolytic activities. The majority were also able to decompose cellulose and xylan, but only a few were able to solubilize inorganic phosphate. Regarding oxalate metabolism, several strains were able to produce calcium oxalate crystals while others decomposed calcium oxalate. These results challenge the current view of the OCP by indicating that fungi are both oxalate producers and degraders. Moreover, they strengthen the importance of the role of fungi in C, N, Ca, and Fe cycles.
47
Suzuki, K., RL Gamble, and SA Sower. "Multiple transcripts encoding lamprey gonadotropin-releasing hormone-I precursors." Journal of Molecular Endocrinology 24, no. 3 (June 1, 2000): 365–76. http://dx.doi.org/10.1677/jme.0.0240365.
Анотація:
The cDNA encoding lamprey prepro-gonadotropin releasing hormone-I (lamprey GnRH-I) has been isolated and sequenced in an agnathan, the sea lamprey, Petromyzon marinus. The lamprey GnRH-I precursor is the first identified in an ancient lineage of vertebrates and has the same overall tripartite structure as other vertebrate GnRH precursors. The amino acid sequence of lamprey GnRH-I and the processing site (Gly-Lys-Arg) are highly conserved during 500 million years of evolution with 60-70% identity compared with those of tetrapod and teleost GnRH precursors. In contrast, the GnRH associated peptide regions are markedly divergent, with less than 20% identity compared with all identified vertebrate precursors. Unlike all other known vertebrate GnRH precursors, which typically have one and in a single case two transcripts, three distinct transcripts were isolated and sequenced in lampreys. These lamprey GnRH-I transcripts, termed GAP49, GAP50 and GAP58, differed in the length of the GAP coding sequence and were demonstrated to be the products of a single gene. Analysis of the lamprey GnRH-I gene intron-2 splice junction demonstrated that alternate splicing produces the different lamprey GnRH-I transcripts. Lamprey GnRH-I is the first GnRH gene demonstrated to utilize splice sequence variants to produce multiple transcripts, which may reflect an ancestral gene regulatory mechanism.
48
Helderman, Tieme, Laurens Deurhof, André Bertran, Sjef Boeren, Like Fokkens, Richard Kormelink, Matthieu Joosten, Marcel Prins, and Harrold van den Burg. "An Isoform of the Eukaryotic Translation Elongation Factor 1A (eEF1a) Acts as a Pro-Viral Factor Required for Tomato Spotted Wilt Virus Disease in Nicotiana benthamiana." Viruses 13, no. 11 (October 30, 2021): 2190. http://dx.doi.org/10.3390/v13112190.
Анотація:
The tripartite genome of the negative-stranded RNA virus Tomato spotted wilt orthotospovirus (TSWV) is assembled, together with two viral proteins, the nucleocapsid protein and the RNA-dependent RNA polymerase, into infectious ribonucleoprotein complexes (RNPs). These two viral proteins are, together, essential for viral replication and transcription, yet our knowledge on the host factors supporting these two processes remains limited. To fill this knowledge gap, the protein composition of viral RNPs collected from TSWV-infected Nicotiana benthamiana plants, and of those collected from a reconstituted TSWV replicon system in the yeast Saccharomyces cerevisiae, was analysed. RNPs obtained from infected plant material were enriched for plant proteins implicated in (i) sugar and phosphate transport and (ii) responses to cellular stress. In contrast, the yeast-derived viral RNPs primarily contained proteins implicated in RNA processing and ribosome biogenesis. The latter suggests that, in yeast, the translational machinery is recruited to these viral RNPs. To examine whether one of these cellular proteins is important for a TSWV infection, the corresponding N. benthamiana genes were targeted for virus-induced gene silencing, and these plants were subsequently challenged with TSWV. This approach revealed four host factors that are important for systemic spread of TSWV and disease symptom development.
49
Ramachandra, Anubama, and Nur Naha Abu Mansor. "Sustainability of community engagement – in the hands of stakeholders?" Education + Training 56, no. 7 (September 2, 2014): 588–98. http://dx.doi.org/10.1108/et-07-2014-0084.
Анотація:
Purpose – The current gap in the field of community engagement is evaluation and measurement of the impacts on the stakeholders, mainly the community being engaged with. The paper aims to discuss these issues. Design/methodology/approach – This paper discusses the need to consider the stakeholder's perspective and their involvement in a community engagement initiative, or in any social program. The authors begin by debating the most common evaluation techniques used, followed by re-introducing stakeholder evaluation to the field of community engagement. Findings – The evaluation using the stakeholders’ approach will not only create a holistic evaluation process, but will also assist in fostering a sense of ownership of the community engagement program. Originality/value – Community engagement is given much importance nowadays in Malaysia, especially in line with institutes of higher learning's tripartite mission, the third mission being the ability to engage with communities. It is not surprising because community engagement offers enormous benefits for regional and societal development. Community engagement relies heavily on partnership and mutual reciprocity between different stakeholders such as communities, universities, non-government organizations, field experts and funding organizations. In order to sustain the engagement initiatives, it is important to know who are the “owners” or stakeholders of the program.
50
A.A. Seitaliyeva. "The State of Teacher Training in the Conditions of Dual Training." Journal of Electrical Systems 20, no. 4s (April 8, 2024): 1036–41. http://dx.doi.org/10.52783/jes.2148.
Анотація:
The purpose of this article is to understand and justify the conditions for the use of elements of the dual training system in the implementation of professional educational programs to improve the efficiency and quality of training of qualified pedagogical personnel in secondary education institutions.
The article discusses the advantages and disadvantages of dual education as a technology for preparing bachelor's degree programs in modern conditions of pedagogical university. Nowadays the topic of "dual training" is a very urgent problem on the pedagogical platform. Because vocational education has never been conceived without connection with the manufacturing sector. The authors describe how dual education is carried out abroad, as well as how the dual education model is being implemented in Kazakhstan. As an example to describe the introduction of elements of dual training, the system of dual training of some pedagogical universities of the Republic of Kazakhstan is presented. The article shows that this technology makes it possible to solve the tasks assigned to the pedagogical university for the training of pedagogical personnel for the system of secondary vocational education. Dual training reduces the gap between theory and practice, promotes the professional development of teaching staff. In addition, it sets new tasks for the higher education system: the development of dual training programs, the regulation of tripartite relations "university – student – employer".