Дисертації з теми "TRIM protein"
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся з топ-44 дисертацій для дослідження на тему "TRIM protein".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Переглядайте дисертації для різних дисциплін та оформлюйте правильно вашу бібліографію.
Najmabadi, Sepideh. "Drosophila model of myosin myopathy rescued by overexpression of a TRIM-protein family member." Thesis, Högskolan i Skövde, Institutionen för hälsa och lärande, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-17919.
Повний текст джерелаZhang, Xuzhe. "Eutherian-specific gene TRIML2 attenuates inflammation in the evolution of placentation." University of Cincinnati / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1573576401238203.
Повний текст джерелаDemange, Antonin. "Protéines à motif tripartite (TRIM) chez le porc (Sus scrofa) et réplication du rétrovirus endogène porcin." Phd thesis, Université Rennes 1, 2013. http://tel.archives-ouvertes.fr/tel-00992386.
Повний текст джерелаFraser, S. J. "Characterising retroviral restriction by TRIM proteins." Thesis, University College London (University of London), 2016. http://discovery.ucl.ac.uk/1532045/.
Повний текст джерелаFekete, Richard Alfred. "Characterizing the protein and DNA interactions of the F plasmid DNA binding protein, TraM." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/NQ60291.pdf.
Повний текст джерелаMarafie, Sulaiman. "TRIM7, a novel binding protein of the mTORC2 component Sin1." Thesis, University of Manchester, 2013. https://www.research.manchester.ac.uk/portal/en/theses/trim7-a-novel-binding-protein-of-the-mtorc2-component-sin1(c344b542-0706-4ec0-be06-ce6683cee52e).html.
Повний текст джерелаSimpson, Shmona. "Genetic, structural, and functional exploration of the restrictive capacity of TRIM proteins against immunodeficiency viruses." Thesis, University of Oxford, 2017. https://ora.ox.ac.uk/objects/uuid:1af588ba-603a-4f39-9443-bb1a95d983f5.
Повний текст джерелаLiu, Sunbin. "Investigation of protein-protein interactions within the human spliceosomal U4/U6.U5 tri-snRNP particle." Doctoral thesis, [S.l.] : [s.n.], 2005. http://webdoc.sub.gwdg.de/diss/2005/liu/liu.pdf.
Повний текст джерелаXin, Gang. "The role of TREM proteins in lung homeostasis and inflammation." Thesis, Imperial College London, 2011. http://hdl.handle.net/10044/1/9058.
Повний текст джерелаRuiz, Echartea Maria Elisa. "Pairwise and Multi-Component Protein-Protein Docking Using Exhaustive Branch-and-Bound Tri-Dimensional Rotational Searches." Electronic Thesis or Diss., Université de Lorraine, 2019. http://www.theses.fr/2019LORR0306.
Повний текст джерелаDetermination of tri-dimensional (3D) structures of protein complexes is crucial to increase research advances on biological processes that help, for instance, to understand the development of diseases and their possible prevention or treatment. The difficulties and high costs of experimental methods to determine protein 3D structures and the importance of protein complexes for research have encouraged the use of computer science for developing tools to help filling this gap, such as protein docking algorithms. The protein docking problem has been studied for over 40 years. However, developing accurate and efficient protein docking algorithms remains a challenging problem due to the size of the search space, the approximate nature of the scoring functions used, and often the inherent flexibility of the protein structures to be docked. This thesis presents an algorithm to rigidly dock proteins using a series of exhaustive 3D branch-and-bound rotational searches in which non-clashing orientations are scored using ATTRACT. The rotational space is represented as a quaternion “π-ball”, which is systematically sub-divided in a “branch-and-bound” manner, allowing efficient pruning of rotations that will give steric clashes. The contribution of this thesis can be described in three main parts as follows. 1) The algorithm called EROS-DOCK to assemble two proteins. It was tested on 173 Docking Benchmark complexes. According to the CAPRI quality criteria, EROS-DOCK typically gives more acceptable or medium quality solutions than ATTRACT and ZDOCK. 2)The extension of the EROS-DOCK algorithm to allow the use of atom-atom or residue-residue distance restraints. The results show that using even just one residue-residue restraint in each interaction interface is sufficient to increase the number of cases with acceptable solutions within the top-10 from 51 to 121 out of 173 pairwise docking cases. Hence, EROS-DOCK offers a new improved search strategy to incorporate experimental data, of which a proof-of-principle using data-driven computational restraints is demonstrated in this thesis, and this might be especially important for multi-body complexes. 3)The extension of the algorithm to dock trimeric complexes. Here, the proposed method is based on the premise that all of the interfaces in a multi-body docking solution should be similar to at least one interface in each of the lists of pairwise docking solutions. The algorithm was tested on a home-made benchmark of 11 three-body cases. Seven complexes obtained at least one acceptable quality solution in the top-50. In future, the EROS-DOCK algorithm can evolve by integrating improved scoring functions and other types of restraints. Moreover, it can be used as a component in elaborate workflows to efficiently solve complex problems of multi-protein assemblies
Ahmadi, Farhana. "Interaction of toll-like receptor 4 with the adaptor proteins MAL and TRAM." Thesis, University of Cambridge, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.610209.
Повний текст джерелаRüßmann, Florian. "The eukaryotic chaperonin TRiC domain-wise folding of multi-domain proteins." Diss., Ludwig-Maximilians-Universität München, 2013. http://nbn-resolving.de/urn:nbn:de:bvb:19-157246.
Повний текст джерелаVillebeck, Laila. "Structural rearrangements of actins interacting with the Chaperonin systems TRiC/Prefoldin and GroEL/ES." Doctoral thesis, Linköping : Univ, 2007. http://www.bibl.liu.se/liupubl/disp/disp2007/tek1099s.pdf.
Повний текст джерелаMcCarthy, Kevin Raymond. "Viral and Host Determinants of Primate Lentivirus Restriction by Old World Primate TRIM5alpha Proteins." Thesis, Harvard University, 2014. http://nrs.harvard.edu/urn-3:HUL.InstRepos:13065027.
Повний текст джерелаGumpper, Kristyn Nicole. "Maintaining Cardiac and Gastric Physiology: TRIM Proteins as Central Factors in Regulation of Organ Homeostasis at the Cellular Level." The Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1563287863754715.
Повний текст джерелаGhoorah, Anisah W. "Extraction de connaissances pour la modélisation tri-dimensionnelle de l'interactome structural." Thesis, Université de Lorraine, 2012. http://www.theses.fr/2012LORR0204/document.
Повний текст джерелаUnderstanding how the protein interactome works at a structural level could provide useful insights into the mechanisms of diseases. Comparative homology modelling and ab initio protein docking are two computational methods for modelling the three-dimensional (3D) structures of protein-protein interactions (PPIs). Previous studies have shown that both methods give significantly better predictions when they incorporate experimental PPI information. However, in general, PPI information is often not available in an easily accessible way, and cannot be re-used by 3D PPI modelling algorithms. Hence, there is currently a need to develop a reliable framework to facilitate the reuse of PPI data. This thesis presents a systematic knowledge-based approach for representing, describing and manipulating 3D interactions to study PPIs on a large scale and to facilitate knowledge-based modelling of protein-protein complexes. The main contributions of this thesis are: (1) it describes an integrated database of non-redundant 3D hetero domain interactions; (2) it presents a novel method of describing and clustering DDIs according to the spatial orientations of the binding partners, thus introducing the notion of "domain family-level binding sites" (DFBS); (3) it proposes a structural classification of DFBSs similar to the CATH classification of protein folds, and it presents a study of secondary structure propensities of DFBSs and interaction preferences; (4) it introduces a systematic case-base reasoning approach to model on a large scale the 3D structures of protein complexes from existing structural DDIs. All these contributions have been made publicly available through a web server (http://kbdock.loria.fr)
Volkmann, Bianca [Verfasser], Thomas [Akademischer Betreuer] Gramberg, Andreas [Gutachter] Burkovski, and Andreas [Gutachter] Burkovski. "The role of TRIM proteins in retrovirus and retroelement restriction / Bianca Volkmann ; Gutachter: Andreas Burkovski, Andreas Burkovski ; Betreuer: Thomas Gramberg." Erlangen : Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 2020. http://d-nb.info/1216332967/34.
Повний текст джерелаZhang, Yanhui, and 张雁惠. "Modulation of transient outward potassium channels by protein tyrosinekinases and demonstration of TRPC and TRPM channels in human atrialmyocytes." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B47161644.
Повний текст джерелаpublished_or_final_version
Medicine
Doctoral
Doctor of Philosophy
Binkley, Sarah L. "Re(I) Tri-Carbonyl Based Radiopharmaceuticals; Synthesis, in vitro Studies, and Protein Complexation." University of Akron / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=akron1469473412.
Повний текст джерелаTAUBENBLATT, PATRICE. "Tri intracellulaire de la vamp et etude de la proteine moteur kif 1a." Paris 11, 2001. http://www.theses.fr/2001PA112142.
Повний текст джерелаDe, Stefano Vittorio. "Origins of specificity in protein modification, reaction patterns of tris-trimesates with human hemoglobin A, water, and n-propylamine." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0015/NQ45655.pdf.
Повний текст джерелаRüßmann, Florian Verfasser], and Franz-Ulrich [Akademischer Betreuer] [Hartl. "The eukaryotic chaperonin TRiC domain-wise folding of multi-domain proteins / Florian Rüßmann. Betreuer: Franz-Ulrich Hartl." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2013. http://d-nb.info/1035066866/34.
Повний текст джерелаRüßmann, Florian [Verfasser], and Franz-Ulrich [Akademischer Betreuer] Hartl. "The eukaryotic chaperonin TRiC domain-wise folding of multi-domain proteins / Florian Rüßmann. Betreuer: Franz-Ulrich Hartl." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2013. http://nbn-resolving.de/urn:nbn:de:bvb:19-157246.
Повний текст джерелаBecher, Peter Moritz [Verfasser]. "Die Bedeutung des TIR-domain-containing adaptor protein inducing Interferon-ß (TRIF) bei der viralen Myokarditis im murinen Tiermodell / Peter Moritz Becher." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2012. http://d-nb.info/1026789435/34.
Повний текст джерелаForsthoefel, David J. "A molecular genetic analysis of the role of the Guanine Nucleotide Exchange Factor Trio during Axon Pathfinding in the Embryonic CNS of Drosophila melanogaster." Connect to resource, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1127241654.
Повний текст джерелаGhoorah, Anisah. "Extraction de Connaissances pour la Modelisation tri-dimensionnelle de l'Interactome Structural." Phd thesis, Université de Lorraine, 2012. http://tel.archives-ouvertes.fr/tel-00762444.
Повний текст джерелаBravo, Silvina Alejandra. "The di/tri-peptide transporters PEPT1 and PEPT2 : expression and regulation in the intestinal Caco-2 and renal SKPT0193 cl.2 cell lines /." Cph. : Department of Pharmaceutics, The Danish University of Pharmaceutical Sciences, 2004. http://www.dfh.dk/phd/defences/silvinabravo.htm.
Повний текст джерелаConfavreux, Antoine. "Optimisation des conditions de migration et de détachement de lignées cancéreuses du cancer du sein en vue de leur tri fonctionnel." Thesis, Lyon 1, 2014. http://www.theses.fr/2014LYO10209/document.
Повний текст джерелаThis thesis investigates migration and detachement properties of different types of breast cancer cell lines : metastatic, invasive MDA-MB-231 and epithelial, low-invasive MCF-7 celles. Videomicroscopy and image analysis techniques were used to obtain dynamic information for large cell populations. Random migration assays performed on MDA-MB-231 cells reveal that their migration properties are related to both medium and surface (substrate adhesion protein type and quantity) conditions. A biphasic behavior for the migrated distance through time was observed to be dependent on the density of adsorbed protein (collagen type IV or fibronectin) on the substrate. Cell shape, detachement and moving properties were also computed as a function of the surface protein characteristics. These results were then used to perform directed migration assays in a chemotactic gradient generated in a microfluidic chamber. Optimal conditions for directed migration of cells were determined by varying the parameters of the system such as gradient maximum concentration, substrate adhesion protein… Lastly, it was experimentally proved that it is possible to separate cancer epithelial cell lines from mesenchymal ones by using chemotactic microfluidic chambers
Dugast, Marc. "Mécanismes de tri impliqués dans le trafic intracellulaire des molécules du CMH II et dans les effets de Nef du VIH-1 sur les CMH et CD4." Paris 7, 2005. http://www.theses.fr/2005PA077016.
Повний текст джерелаSchächterle, Carolin. "Der strukturelle und funktionelle Einfluss des Cytokins IFNgamma auf die Modulation proteasomaler Komplexsubtypen." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2013. http://dx.doi.org/10.18452/16849.
Повний текст джерелаThe 20S proteasome is the core element of the ubiquitin-proteasome-system, which degrades defective, unneeded and oxidized proteins, while three catalytically active subunits hydrolyze the peptide bonds of the polypeptide. The proinflammatory cytokine IFNg induces the expression and incorporation of three alternative, catalytically active immunosubunits resulting in variable isoforms of the 20S proteasome. The additional association of the 19S regulator, or the PA28 and PA200 activator, respectively, expands the range of proteasome complex subtypes. The time course of IFNg stimulation showed that the proteasomal association of PA28 and PA200 occurs antagonistically, forming low molecular weight complex subtypes. Furthermore, this study revealed for the first time an IFNg dependent association of the PA200 monomer to the 20S proteasome. Ex vivo experiments showed that the deficiency of the immunosubunit LMP7 is compensated by the association of the PA28 activator to the 20S-19S proteasome, whereas the functional efficacy remains elusive. In a monocytic cell line, a chymotryptic active complex with a very high molecular weight was detected, and mass spectrometry confirmed proteasomal subunits and components of the protein synthesis machinery, suggesting an association of the proteasome with the polysome. The fact of cotranslational degradation may also explain the association of the chaperonin TriC, an ATP dependent protein folding chaperonin. Electron micrographs could reveal that TriC possibly interacts directly with the proteasome. Next to the new structural results, the functional analysis confirmed the degradation of polyubiquitinated substrates by 19S regulator associated complex subtypes, and in addition to it, the 19S-20S-19S proteasome degraded and deubiquitinated the model substrate HA-Ubi-IkBa-flag better than the 20S-19S proteasome.
Ecard, Jason. "Mécanismes de tri et voies de transport intracellulaire des protéines de la membrane du lysosome." Electronic Thesis or Diss., Sorbonne université, 2019. https://accesdistant.sorbonne-universite.fr/login?url=https://theses-intra.sorbonne-universite.fr/2019SORUS094.pdf.
Повний текст джерелаMore than sixty different proteins are inserted into the membrane of the lysosome, participating in the various functions of this organelle. But the intracellular trafficking pathways that lead lysosomal membrane proteins (LMPs) to this organelle are not well understood. Interestingly, some LMPs exhibit abnormal intracellular localisations in some cancers. This is the case of the glycoprotein LAMP1 which is overexpressed at the cell surface in several cancers, from where it plays several roles in tumour aggressiveness. Thanks to the Retention Using Selective Hooks (RUSH) system, allowing the synchronisation of the transport along the secretory pathway, we have shown that neosynthesized LAMP1 reaches the plasma membrane before entering endosomes. Comparing the routes followed by different LMPs also revealed that LAMP1 and LIMP2 are sorted at the Golgi apparatus, LIMP2 being concentrated in characteristic vesicular and clathrin-free structures. We have also shown that, surprisingly, this sorting at the level of the Golgi apparatus is independent of clathrin adapters recruitment signals carried in the C-terminal tails of these two LMPs. To investigate what mechanisms might be involved in the overexposure of LAMP1 at the cell surface of certain cancers, we also performed a gene knock-out screen based on CRISPR-Cas9 technology. We selected genes whose inactivation affected LAMP1 levels at the surface and obtained many candidate genes that are under study
Collins, Karl Daniel. "The development and application of radical and anionic cyclisations mediated by samarium(II) diodide and protic co-solvents." Thesis, University of Manchester, 2012. https://www.research.manchester.ac.uk/portal/en/theses/the-development-and-application-of-radical-and-anionic-cyclisations-mediated-by-samariumii-diodide-and-protic-cosolvents(0e807a8c-d40f-45bb-8d83-26e79ebe9388).html.
Повний текст джерелаHuang, Shu-Yu, and 黃書毓. "Structural Characterization and Molecular Interaction with the TRIM Domains of Promyelocytic Leukemia Protein." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/40271052944316786419.
Повний текст джерела國立臺灣大學
生化科學研究所
103
PML protein is mainly present inside the nucleus in the form of PML nuclear bodies (PML-NBs) and serves as a SUMO E3 ligase which facilitates SUMOylation on PML itself and proteins in PML-NBs. PML has a conserved N-terminal TRIM/RBCC region comprised of a RING finger domain, two B-boxes and a coiled-coil region. The purpose of this study is to investigate the structural basis of SUMOylation on PML TRIM domains which is essential in the formation of PML-NBs. However, not much of the structural information of PML is known today. In this work we solved the structures of RING finger and B-box 1 of dimer by advanced NMR techniques. We identified the residues involved in the binding of SUMO E2 enzyme Ubc9 and PML TRIM domains. The result of SPR indicated that dimeric domain RB1 showed much stronger interaction with Ubc9 (KD=16uM) than any single domain (KD=490uM for RING finger and KD=94uM for B-box 1), suggesting that RING finger and B-box 1 bound with Ubc9 in synergy. Here, we provide the structures of PML TRIM domains and the bindings with Ubc9 at molecular level in order to understand the SUMOylation mechanism on PML TRIM domains.
Späth, Kerstin [Verfasser]. "Untersuchungen zur funktionellen Charakterisierung von regulatory-protein T-lymphocyte-1 (rpt-1, Trim 30) / vorgelegt von Kerstin Späth." 2005. http://d-nb.info/973945087/34.
Повний текст джерелаChang, Chia-Ling, and 張嘉玲. "I. Studies of a Rice Sterile Mutant sstl from Taiwan Rice Insertional Mutants (TRIM) CollectionII. Multifunctional Roles of Rice Yellow Mottle Virus (RYMV) P1 Protein in Host Infection." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/dbh86b.
Повний текст джерела國立臺灣大學
農藝學研究所
107
Rice (Oryza sativa) is one of the main crops in the world. By 2025, more than 3.9 billion people count rice as daily food energy especially in Asia and Africa. Because of the increased population, the production of rice is still far away behind requirements. Meanwhile, the food safety issue related to feed the world continuously, to adjust crop adaptation in climate change, to establish sustainable agriculture, etc. are urgent to be solved. My study in this thesis will cover two different topics described as followings: First, studies of a rice sterile mutant (semi-sterile; sstl) from the TRIM collection and the second investigation, multifunctional roles of rice yellow mottle virus (RYMV) P1 protein in host infection. I. Studies of a rice sterile mutant sstl from the TRIM collection Sterility significantly affects rice production and leads to yield defects. The undeveloped anthers or abnormal pollens represent serious defects in rice male sterility. Therefore, understanding the mechanism of male sterility is an important task. Here, we identified an untagged T-DNA insertion mutant sstl based on its semi-sterile morphology of rice development. We investigated the fully sterile mutants (sstl-s) of sstl segregated progeny showed defective pollens and abnormal anthers. Transcriptomic analysis of sterile sstl-s revealed significant differences in several biosynthesis pathways, such as downregulated cell wall, lipids, secondary metabolism, and starch synthesis. The downregulation of gene expression is consistent with the morphological characterization of sstl-s anthers with irregular exine, absence of intine, lack of starch accumulation in pollen grains and no accumulation of flavonoids in anthers. Moreover, defective microsporangia development led to abnormal anther locule and aborted microspores. The downregulated expression of lipids, starch, and cell wall synthesis-related genes resulted in loss of fertility. In summary, the analysis of sstl-s mutant pointed out the importance of microsporangia in the development of anthers and functional microspores. II. Multifunctional roles of RYMV P1 protein in host infection Rice yellow mottle virus (RYMV), a virus spread all over African countries, has caused significant damage to rice plant and resulted in dramatic decrease of yield in Africa. ORF1 of RYMV encodes P1 protein which belongs to a novel zinc finger family with its conformation regulated by redox switches. P1 protein was suggested as suppressor after virus infection; however, the function of P1 in virus transition is still unclear. In the present study, we explored the possible mechanisms of P1 in virus replication rate, movement ability, and suppression of host defense. We showed that mutated P1 RYMV still could replicate but at less replication rate than that of normal RYMV after transfection into rice protoplast. All replication rate with mutated P1 RYMV infection decreased from 24 hrs to 48 hrs after inoculation. This result indicated that functional P1 not only could regulate RYMV replication production but also maintain viral RNA transcription stability. In addition, mutated P1 RYMV showed no virus accumulation after infection into rice plant and none of the infected plant displayed virus accumulation. This indicated P1 could help regulate virus spread after infection. P1 protein co-localized with ER marker suggested this protein might participate in virus spread in the cell. Meanwhile, P1-eGFP fusion protein was transmitted between two adjacent cells and thus illustrating P1 mobility. Heat-inducible transgenic rice also displayed P1 transcript moving from lower leaf to non-heat treated upper leaf after heat treatment. This evidence strongly suggested that P1 RNA could be involved in virus long-distance movement. In additional to virus infection ability, the virulence can also be affected by host defense. Our data suggested functional P1 protein might help virus resist host defense by decreasing the expression of biotic-related genes such as PR10A and CPR5. Moreover, P1 could hijack host silencing pathway through down-regulating DCL-like and AGO expression to make host susceptible. Taken together, these results illustrate that P1 protein may serve as multifunctional roles to participate in virus replication, movement, as well as counterattack.
Chang, Jian-Sheng, and 張建盛. "Protein Expression, Purification, and Structural Characterization of Truncated Human TREM-Like Transcript-2." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/39999279592359434152.
Повний текст джерела國立清華大學
生物資訊與結構生物研究所
95
TREMs, triggering receptor expressed on myeloid cells, belong to a rapidly expanding family of receptors that include activating and inhibitory isoforms encoded by a gene cluster linked to the major histocompatibility complex (MHC) on human chromosome 6. Human TREM-like transcript-2 (hTLT-2), one kind of TREM gene family with 321 amino acids, is a membrane protein and signal receptor expressed on neutrophils, macrophages, and B lymphocytes in innate and adaptive immune system. Until now, the characterization and structural analysis of hTLT-2 remain unclear. In this study, the N-terminus extracellular domain of hTLT-2 was truncated to 26-kDa (from 14-Q to 265-D) and 13-kDa (from 14-Q to 132-N) fragments and cloned into expression vectors pET-28a and pGEX-6P-3. The two partial fragments of hTLT-2 were expressed in competent cells, such as E. coli BL21 (DE3), BL21-Gold (DE3), and Rosetta (DE3). Tag of target proteins were His-tag and GST-tag and the two truncated hTLT-2 proteins were purified with Ni-column and GSTrap FF column, respectively. Guanidine hydrochloride (Gdn-HCl) was used to dissolving the truncated hTLT-2 involved in inclusion body and the target proteins were refolded by dialysis for removing Gdn-HCl in the buffer. The results demonstrate that the larger size of 26-kDa of outer-membrane region of truncated hTLT-2 proteins are difficult to be expressed in E. coli expression system and tend to aggregate with other proteins in inclusion body. According to circular dichroism spectrum, the secondary structure of this 16-kDa of His-tagged hTLT-2 fragment is composed of about 5% alpha helix, 47% beta sheet, and 48% random coil. Thus, the secondary structure of outer-membrane region of 16-kDa of truncated hTLT-2 like TREM-1 and TLT-1 of TREM family mainly comprises beta sheets.
Lin, Yin-Ling, and 林易鈴. "A Trip to Protect Your Heart:Studies of Cardiovascular Nursing Care." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/qx42zw.
Повний текст джерела國立陽明大學
科技與社會研究所
106
This study explores cardiovascular nursing care from the perspectives of gender and technology studies. I review studies of cardiovascular epidemiology, medical guideline of heart diseases, and cardiovascular nursing education in school and the hospital. I also review literatures of nurse-patient relationship, nursing guidance, and "gender innovation" studies on female heart diseases. I use multiple research methods in this studies. The empirical data in this study includes my own clinical practice of cardiovascular nursing care, and in-depth interviews of my colleagues. Secondary data includes health education information of cardiovascular nursing care collected from five teaching hospitals. The contents of this thesis is divided into three chapters. In Chapter II, I describe the clinical treatments of heart disease in Taiwan. In Chapter III, I compare the differences between school nursing education and clinical practice in cardiology. I use pain assessment in cardiology for an example to explore gender differences in cardiology. In Chapter IV, I analyze the health education information of cardiovascular care from five medical centers. I analyze and compare their writers, forms of transmission, timing of use, and their differences concerning sex, gender and gender differences. I conclude my thesis with "seeing your heart" and " put your heart in my heart". I believe that the perspectives of gender and technology studies can lead us to a better cardiovascular nursing care.
Liu, Sunbin [Verfasser]. "Investigation of protein-protein interactions within the human spliceosomal U4/U6.U5 tri-snRNP particle / vorgelegt von Sunbin Liu." 2005. http://d-nb.info/97621802X/34.
Повний текст джерелаRold, Christopher James. "The role of the cellular proteasome and ubiquitin in post-entry restriction of retroviruses by TRIM5[alpha]." Diss., 2009. http://etd.library.vanderbilt.edu/available/etd-03302009-150129/.
Повний текст джерелаChang, Po-Chih, and 張博智. "Synthesis and Reactivity Study of Tris(1-pyrazolyl)methane Copper(I) Complexes Relating to the Copper Protein Active Site Modeling Complexes." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/19584927692376547912.
Повний текст джерела國立中山大學
化學系研究所
92
Nitrous oxide is a greenhouse gas produced in large quantity by several industrial processes. Efficient means of eliminating N2O are therefore of interest. The denitrification enzyme nitrous oxide reductase (N2OR), which reduces N2O to N2 and water , has recently been shown to contain an unprecedented [Cu4-µ4S] active site. Multinuclear copper sulfide compounds are known but have not been studied in the context of modeling N2OR or as N2O reduction catalysts. The synthesis of new tetranuclear [Cu4-µ4S] compounds is proposed to model the N2OR active site.The purpose of our research is to synthesize [Cu2-µ2S] complex, which original compound of [Cu4-µ4S] complex. This can be groundwork for mimicking the copper protein active site.
Choonara, Bibi Fatima. "A Tri-Functionalized Oral Core-Melt Tablet (OCMT) for enhanced delivery of gastro-sensitive proteins and synthetic peptides." Thesis, 2017. https://hdl.handle.net/10539/24911.
Повний текст джерелаThe global pharmaceutical biotechnology industry is continually growing and increased amounts of protein and peptide-based therapeutics are entering into the market. Conventionally, these therapeutic proteins and peptides are administered intravenously, subcutaneously or intramuscularly as oral routes of administration may result in their degradation in the gastrointestinal tract (O’ Connor, 2009). The parenteral route limits patient acceptability and convenience. Oral dosage forms, particularly tablets, are considered one of the most common and widely used routes of drug administration and accounts for approximately 50% of all dosage forms on the market (Oh et al., 2012). Thus, the development of oral technologies for therapeutic proteins and peptides remain a dynamic research field despite its many challenges. Successful oral delivery of proteins and peptides require the accomplishment of three key tasks: protection of the macromolecules from degradation in the gastrointestinal tract (GIT), permeation through the intestinal barrier and the absorption of molecules into the systemic circulation. Currently, no clinically useful oral formulations have been approved but several attempts have been made to overcome the challenges of low oral bioavailability resulting from poor absorption, poor permeation and enzymatic degradation of the proteins and peptides in the GIT. Present strategies attempt to provide structural protection of the proteins and peptides and improved absorption through the use of enzyme inhibitors, absorption enhancers, novel polymeric delivery systems and chemical modification. However, each of these technologies possesses limitations that preclude the successful oral delivery of proteins and peptides. The design and development of the novel Tri-functionalized OCMT attempts to breakthrough this market by triple targeting the major challenges governing successful oral delivery of proteins and peptides. Essentially, the Tri-functionalized OCMT is made up of three distinct functional components; the core-eutectic region, the outer polymer shell and the ‘smart’ polymeric sheet. Triple targeting was achieved by incorporating the bioactive into a core-melt archetype within the tablet and facilitating the process of in situ crosslinking which significantly affects the way the bioactive is protected within the compressed tablet matrix as well providing desirable bioactive release kinetics. In addition, the incorporation of a co-inhibitory ‘smart’ polymeric sheet provided added protection and enabled an improved absorption by virtue of its intrinsic properties and co-inhibition of the major determinants, CYP3A4 and Pgp, of poor absorption and low oral bioavailability. Lastly, targeting of enzymatic degradation was achieved by incorporation of a pH modifier that functions to transiently lower the micro-environmental pH and reduce the optimal environment for enzyme activity. The combination of the distinct formulatory components contained within the Tri-functionalized OCMT was extensively evaluated through in-depth in vitro physicochemical and physicomechanical characterization, ex vivo analyses and in vivo performance. In vitro and ex vivo characterization enabled the optimization of design variables and promoted the achievement of desirable functionalities for the attainment of optimum in vivo performance. In vivo analyses of the Tri-functionalized OCMT in the Large White pig model revealed an enhanced oral bioavailability of the incorporated peptides, octreotide and exenatide, following oral administration, as compared to their conventionally administered subcutaneous counterparts. In addition, pharmacokinetic analysis of the Tri-functionalized OCMT confirmed the maintenance of sustained, therapeutic levels of both octreotide and exenatide over the 24 hour period, supporting a convenient once-daily dosing. The rate at which protein and peptide-based therapeutics are developing is astounding, and this consequently increases the demand and focus towards achieving a simpler and more effective oral delivery of therapeutic proteins and peptides. The Tri-functionalized OCMT has successfully demonstrated its feasibility in enhancing the oral delivery of therapeutic proteins and peptides up to a preclinical stage. It may potentially serve as a viable alternative to the conventional parenteral route of administration for the treatment of various disease conditions by incorporating a multitude of proteins and peptides into its versatile design. The advancement of the Tri-functionalized OCMT to the clinical stage and beyond may ultimately improve patient outcomes and change the face of therapeutic protein and peptide delivery globally.
LG2018
Häcker, Irina. "Electron microscopic localization of tagged proteins in the yeast S. cerevisiae spliceosomal U4/U6.U5 trisnRNP." Doctoral thesis, 2008. http://hdl.handle.net/11858/00-1735-0000-0006-AD0F-E.
Повний текст джерелаNarita, Vanny. "Molecular, genetic, and functional analysis of Ptr3p, a novel protein involved in amino acid and dipeptide regulation of di/tri-peptide transport system in Saccharomyces cerevisiae." 2002. http://etd.utk.edu/2002/NaritaVanny.pdf.
Повний текст джерелаTitle from title page screen (viewed Oct. 3, 2002). Thesis advisor: Jeffrey M. Becker. Document formatted into pages (xiv, 250 p. : ill. (some col.)). Vita. Includes bibliographical references.
Müllers, Nina. "Struktur-Funtionsbeziehung in den spleißosomalen Proteinen des U4/U6•U5 tri-snRNP." Doctoral thesis, 2006. http://hdl.handle.net/11858/00-1735-0000-0006-AC43-D.
Повний текст джерела