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1
Lynch, Thomas John. "Adult stem cells in the trachea and tracheal submucosal glands." Diss., University of Iowa, 2016. https://ir.uiowa.edu/etd/6464.
Повний текст джерелаАнотація:
Breathing is essential for human life, yet tens of millions of people in the U.S. alone suffer from lung diseases. With each breath, lungs are exposed to the external environment. Inhaled air first passes through the trachea, bronchi, and finally the bronchioles before it reaches the alveoli where gases are exchanged. A barrier of epithelial cells protects the airways. In addition, epithelial glands also secrete protein-rich fluids onto the airway surfaces to help maintain sterility. Injury, disease, or other factors can damage these cells, and regiospecific stem cells (SCs) can divide to replace them. However, many important details about lung SCs are still unknown. For example, what processes control SC division? How do region-specific SCs differ from one another? And how does disease or injury impact SC biology? We found that some processes that regulate lung development also control adult SC division following injury. We show that SCs from airway glands give rise to surface epithelial cell types and glandular cell types. In contrast, surface SCs only generated surface cell types. Finally, we identify a type of cell in the glands that can regenerate surface cell types after severe injury. These studies provide new insights into the neighborhoods in which SCs reside in the large airways and processes that control their contribution to airway repair following injury. Overall, this research provides important new insights into adult SC biology and conditions affecting lung health.
2
Yamashita, Masaru. "Regeneration of the larynx and trachea." Kyoto University, 2007. http://hdl.handle.net/2433/135671.
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3
Sellitri, Francesco <1968>. "Trapianto eterotopico della trachea: studio sperimentale." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2007. http://amsdottorato.unibo.it/447/1/Tesi_Sellitri_Francesco.pdf.
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4
Sellitri, Francesco <1968>. "Trapianto eterotopico della trachea: studio sperimentale." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2007. http://amsdottorato.unibo.it/447/.
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5
Sugano, Yoshimi Teresa. "Bronchoskopische Vermessung und dreidimensionale Darstellung der Trachea." Doctoral thesis, Humboldt-Universität zu Berlin, Medizinische Fakultät - Universitätsklinikum Charité, 2006. http://dx.doi.org/10.18452/15419.
Повний текст джерелаАнотація:
Im Rahmen der vorliegenden Arbeit ist ein neues bronchoskopisches Verfahren entwickelt und getestet worden, mit dem eine Längs- und Querschnittsvermessung zentraler Atemwegesstenosen in Echtzeit ermöglicht wird und anschließend eine 3D-Rekonstruktion des untersuchten Abschnitts dargestellt werden kann. Für die Querschnittsflächenbestimmung wird durch den Arbeitskanal des Bronchoskops eine Lasersonde eingeführt und ein Laserlichtring auf die Trachealwand projiziert. Die Abbildung des Lichtrings wird im bronchoskopischen Bild noch während der Untersuchung mit Hilfe einer speziell im Rahmen dieser Arbeit entwickelten Software Endo3D segmentiert und vermessen. Durch Speichern aufeinanderfolgender Querschnittsflächen kann ein 3D-Datensatz erstellt, visualisiert und das Volumen berechnet werden. Experimentell wurde die Methode an Kunststoffmodellen mit bekannten Maßen und Präparaten aus Schweineluftröhren getestet. Die Referenzwerte für die Volumen der Schweineluftröhren wurden durch Wasservolumetrie bestimmt. Klinisch wurde die Methode in einer Pilotstudie getestet. Bei 10 Patienten wurden Querschnitte und Volumen unterschiedlich langer Trachealabschnitte vermessen. Als Vergleichsmethode wurde jeweils eine CT durchgeführt. Die Ergebnisse im experimentellen Teil zeigten bei sehr guter Reproduzierbarkeit eine gute Korrelation zwischen den bronchoskopisch gemessenen Werten und den realen bzw. Referenzwerten. Die Korrelation der klinischen Ergebnisse erwies sich als befriedigend ohne Hinweis auf einen systematischen Fehler. Ein kleiner systematischer Messfehler im experimentellen Teil zeigte sich als irrelevant für die Klinik. Die hier vorgestellte Methode ermöglicht untersucherunabhängige Verlaufsbeschreibungen von Stenosen und erlaubt die Schaffung einer einheitlichen Klassifikation. Darüber hinaus ist damit zukünftig eine individualisierten Stentimplantation sowie Tumorvolumenberechnungen denkbar.In this paper a new bronchoscopic method was developed and tested, that performs measuring both cross-sectional areas and length of central airway stenoses in real-time. Furthemore this method enables to represent a three-dimensional reconstruction of the airway section that was analysed. To measure th cross-section area, a laser probe inserted into the operating channel of a bronchoscope projects a ring of light onto the trachal wall and marks the cross-sectional area. A new software especially developed for this method makes it possible to identify the projected ring of light and measures the cross-sectional area after applying lens distortion correction algorithms. By saving a succession of cross-sections 3D-data is provided for visualizing and volume calculation. The measuring accurracy was first tested employing plastic tubes with known diameters and 17 models of porcine trachea. The cilinical evaluation was realized in a pilot study. Sections of different length of tracheas of 10 patients were analysed by both the new method and by CT. The results of the experimental part showed good correlation between the reference methods and a very good reliability. The correlation between CT and bronchoscopic measuring results was slightly less good than the experimental correlation, but they showed no systematic measuring error. A little systematic error in the experimental evaluation prooved to be irrelevant for clinical results. The new method enabels objective description of stenoses and makes it possible to develop a standardized classification. In future indvidual stent construcions or calculation of tumor volumes are conceivable.
6
Okuyama, Hideaki. "Transplantation of multiciliated airway cells derived from human iPS cells using an artificial tracheal patch into rat trachea." Kyoto University, 2020. http://hdl.handle.net/2433/253142.
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7
Hemphälä, Johanna. "Genetic dissection of tubulogenesis in the Drosophila trachea /." Stockholm : Department of Developmental Biology, Wenner-Gren institute, Stockholm University, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-430.
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8
Khawaja, Aamir Mahmoud. "Inhibition of neurogenic mucus secretion in ferret trachea." Thesis, Imperial College London, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.286452.
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9
Sakaguchi, Yasuto. "Development of a novel tissue-engineered nitinol-frame artificial trachea with native-like physical characteristics." Kyoto University, 2018. http://hdl.handle.net/2433/235063.
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10
Karkhanis, Teja. "Intra-animal and Inter-animal Variations in the Biomechanical Properties of Tracheal Cartilage Rings." University of Cincinnati / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1448037942.
Повний текст джерела
11
Hermawan, Vera. "Experimental techniques to determine the Young's Modulus of the trachea thesis submitted in partial fulfilment of the degree of Master of Engineering, Auckland University of Technology, September 2004." Full thesis. Abstract, 2004.
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12
Amoako, Daniel Kwasi. "Cyclopiazonic acid changes the mode of excitation-contraction coupling in acetylcholine-stimulated bovine tracheal smooth muscle /." Hong Kong : University of Hong Kong, 1996. http://sunzi.lib.hku.hk/hkuto/record.jsp?B1865017X.
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13
Ramnarine, Sean Issac. "Neurogenic mucus secretion in ferret trachea : excitatory and inhibitory control." Thesis, Imperial College London, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338873.
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14
鄧兆庭 and Siu-ting Alvin Tang. "Implementation and evaluation of evidence-based practice guidelines for open endotracheal suctioning in mechanically-ventilated adult patients." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/193027.
Повний текст джерелаАнотація:
Endotracheal suctioning is a procedure performed on a daily basis in hospitals, and is mostly take place in intensive care units (ICUs). (Annapoorna, 2005; Day et al, 2009). It helps removing sputum or secretion out from patients’ trachea. For patients who are under mechanical ventilation, this procedure is vital to maintain their airway patency when they are intubated with endotracheal tube or tracheostomized (Finucane & Santora, 2003). However, the procedure has its own risk and complications such as hypoxaemia, atelectasis, cardiovascular instability and more (Thomson, 2000). There are in general two types of endotracheal suctioning: open and closed system. As disconnection of mechanical ventilation from patients is needed for open endotracheal suctioning (OES), it has a higher risk of complications. However, the cost for OES is much cheaper compared to the closed system. Although OES is widely used in Hong Kong, there is no evidence-based guideline for nurses to follow. The guideline developed by American Association of Respiratory Care (2010) is lack of specificity on the target population and its recommendations were based on mixed literatures targeting on adult and infant patients. Therefore, the aim of this dissertation is to develop an evidence-based guideline for OES in adult patients under mechanical ventilation in ICU.
To develop a guideline for OES, search was performed in multiple electronic databases (British Nursing Index, CINAHL, Cochrane Library, Ovid MEDLINE, and PubMed) with keywords related to OES and its complications. A total of 457 studies fulfilled the inclusion criteria and 11 of them were selected. The selected studies were evaluated by quality appraisal checklists, which are developed by Scottish Intercollegiate Guidelines Network (SIGN). Data were extracted for developing the guideline.
Evidence have shown that the incidence of post-OES hypoxemia can be reduced by performing hyperoxygenation with 100% oxygen for 4-6 breaths prior and after each open endotracheal suction, accompanying with hyperinflation with 150% of patient’s tidal volume at most 8 breaths/40 seconds delivered by ventilator and prohibiting normal saline instillation into trachea for diluting the sputum. The grades of the recommendations in the guideline were rated with using of the SIGN grading system. The implementation potential was analyzed by the patients’ characteristics, transferability of the findings, feasibility of implementation and cost-benefit ratio.
A 12-month implementation program was developed including communication with stakeholders, 4-week pilot testing, and training of ICU staffs, and implementation of OES guideline. The effectiveness of the guideline will be evaluated based on the primary outcome (i.e. oxygen level in blood) for detecting the incidence of hypoxemia. Also, the acceptability of the guideline, compliance of the guideline, financial cost reduction and better quality of service will be used as other evaluation indicators.published_or_final_version
Nursing Studies
Master
Master of Nursing
15
Kurujareon, Jutarat. "Simulation of airflow in the human tracheobronchial network." Thesis, University of Hertfordshire, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.391374.
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16
Боханов, Г. Ю., та В. Ф. Кривецький. "Моделювання аллопротезу для оптимізації циркулярної подовженої резекції трахеї". Thesis, Сумський державний університет, 2017. http://essuir.sumdu.edu.ua/handle/123456789/57997.
Повний текст джерелаАнотація:
При видаленні великих фрагментів трахеї відновлення її цілісності оптимальним способом анастомозування «кінець в кінець» виникають труднощі, особливо при необхідності корекції рестенозу. Полегшити хід операції можна шляхом протезування. Разом з тим, питання вибору матеріалу для трахеального протеза залишається невирішеним та актуальним.
17
Fabre, Dominique. "Reconstruction trachéale autologue." Thesis, Paris 11, 2015. http://www.theses.fr/2015PA114835.
Повний текст джерелаАнотація:
La reconstruction trachéale autologue a pu être standardisée et optimisée grâce au développement d’untravail de recherche expérimentale. Ce travail a permis d’améliorer la technique chirurgicale et les résultatscliniques. C’est une solution thérapeutique qui permet de remplacer de façon reproductible plus de 50 % delongueur de trachée. Toutes les autres techniques de remplacement trachéal développées par denombreuses équipes, ainsi que leurs résultats expérimentaux et cliniques, ont été étudiés et classés enfonction du substitut utilisé.Après de nombreuses publications du Laboratoire de Recherche du Centre Chirurgical Marie Lannelonguesur ce sujet, nous nous sommes orientés vers l’utilisation des tissus autologues (lambeau libre et cartilagescostaux) en collaboration avec les chirurgiens plasticiens. Cela a permis grâce aux avancées techniques etnotamment de microchirurgie de réaliser un nouveau conduit trachéal. Ce substitut ne contient aucunmatériel prothétique et il peut donc résister aux infections. Malgré l’absence de renfort prothétique, il résisteaux pressions et surtout à la dépression respiratoire.Le développement de cette technique chirurgicale a été obtenu grace à ces travaux de recherche qui ontpermis d’optimiser la procédure, de l'améliorer et de la répéter.Le premier travail expérimental a été la création d'un modèle animal de remplacement trachéal autologuepar un lambeau pédiculé armé. Nous avons ainsi confirmé la résistance mécanique et la viabilité descartilages, ainsi que la durabilité de ce type de reconstruction.Les travaux suivants ont été réalisés en collaboration avec des équipes spécialisées en Ingénierie tissulaire.Le premier travail expérimental d’Ingénierie tissulaire a consisté en la réalisation d’anneaux de cartilage àpartir de cellules souches. L'objectif était d'obtenir des anneaux cartilagineux préformés, que l'on pourraitinsérer dans l'épaisseur du lambeau.Le deuxième travail a été de développer une technique permettant de transformer le revêtement cutané dulambeau en une muqueuse respiratoire. Quatre techniques d'ingénierie tissulaire ont été utilisées et testées :la greffe de péritoine, la greffe de muqueuse buccale expansée, l’ensemencement de cellules de lamuqueuse trachéale et la greffe de cellules épithéliales respiratoire de culture.Malgré les avancées technologiques, la culture de cellules cartilagineuses en trois dimensions sur desmoules en Silicone ne s’est pas avérée satisfaisante.Les différents procédés de remplacement du revêtement cutané ont confirmé la nécessité de réaliser desétapes supplémentaires au préalable de l'intervention chirurgicale. Parmi les techniques de remplacementde l'épithélium, la greffe de muqueuse buccale expansée et la greffe d’épithélium respiratoire de culture ontété les plus fiables.La reconstruction autologue est à ce jour la meilleure alternative pour le remplacement trachéal étendu enutilisant des tissus autologues. La poursuite d’une approche expérimentale est fondamentale pourl’amélioration de nos résultats. Ainsi, ces travaux vont être poursuivis par un travail expérimental sur leremplacement des anneaux de cartilages par des anneaux de Titane poreux sur un modèle animalAutologous tracheal reconstruction has been standardized and optimized in parallel with the development of experimental research and clinical practice. This is a therapeutic solution that replaces reproducibly more than 50% of tracheallength. All the other tracheal replacement techniques, developed by many teams and their experimental and clinical results, were studied and classified according to the substitute used.After an experimental study at the Laboratory of Surgical Research in the Marie Lannelongue, center, we started to work towards the use of autologous tissue (free flaps and costal cartilages). Thoses tissues were shaped using technical advances including microsurgery to create a new tracheal conduit.This tracheal substitute does not contain any prosthetic material. It may therefore resist to infection and it resists to respiratory pressures and especially respiratory depression.The development of this technique was conducted in parallel with research studies, that improved and optimized the surgical process and the results.The first experimental work was the creation of an animal model tracheal replacement using an armed autologous pedicle flap. We confirmed the strength and viability of the cartilage strip inserted between the dermal layers and the sustainability of this type of reconstruction.The following work was carried out in collaboration with two other teams specialized in Tissue Engineering.The first experimental work of tissue engineering tried to produce cartilage rings from stem cells. The objective was to obtain complete cartilaginous rings that could be inserted into the thickness of the flap.The next objective was to develop a technique to transform the superficial layer of the skin in a respiratory epithelium. Four tissue engineering techniques were used and tested: peritoneum of the graft, oral mucosa transplant, seeding cells from the tracheal mucosa and graft culture respiratory epithelial cells.Despite advances in technology, the culture of cartilage cells in three dimensions on Silicone mold were not viable and could not be used in clinical practice.The various alternative processes of skin covering highlighted the need for additional steps in advance of surgery.Among the techniques used to replace the epithelium with an expanded graft buccal mucosa, transplantation of culture respiratory epithelium is the most reliable.Autologous reconstruction is so far the best alternative for extended tracheal replacement using only autologous tissue. The pursuit of an experimental approach is fundamental to improve our results.Thus, this work will be pursued by an experimental work on the replacement of cartilage rings using porous titanium ring on an animal model
18
Nunes, Sandro Filipe Fernandes. "Influenza A infection dynamics in an Ex vivo organ culture of pig trachea." Thesis, University of Cambridge, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609311.
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19
Partington, L. "Development of a pressurised transmural decellularisation method for application in tissue engineering trachea." Thesis, University College London (University of London), 2015. http://discovery.ucl.ac.uk/1465826/.
Повний текст джерелаАнотація:
Tracheal abnormalities, congenital or acquired, represent a currently unmet clinical need. Tissue engineering has recently advanced and has been used to engineer hollow organs, including tracheae, for clinical use on a compassionate basis. However the current clinically used method for tracheal decellularisation has received mixed success and requires development to achieve GMP translation, quality manufacturing standards and ultimately routine clinical use. This thesis first examined the current clinically used detergent-enzymatic method (DEM) of decellularisation, a highly manual process that takes twenty-eight days to complete. Although the method achieved full decellularisation of non-cartilaginous regions of the tracheae, it failed to reduce the donor nuclear material sufficiently and resulted in the loss of key biochemical components, glycosaminoglycans (GAG) and collagen Type II, and the loss of biomechanical strength. A novel method for rapid, effective and non-detrimental tracheal decellularisation was required. Pressurised transmural flow was hypothesised to meet those requirements. A dual chamber bioreactor system was designed, fabricated and optimised to enable pressurised transmural decellularisation (PTD) to be investigated. Optimal PTD process parameters were ascertained and shown to produce tracheal scaffolds that achieved full decellularisation of the non-cartilaginous regions of the tracheae, a reduction of donor nuclear material (95%) which met the currently recommended levels of residual donor DNA for tissue engineered products, as well as maintaining GAG, collagen and biomechanical strength at comparable levels to the native tracheae. Additional to this, the new PTD process achieved this effective and non-detrimental decellularisation of tracheae in five working days with a ten-fold cost of goods reduction. With further development, the PTD method could become a fully automatable and closed process which could progress tissue engineered tracheae towards becoming a validated and regulated advanced therapy medicinal product (ATMP) and enable the advancement of tissue-engineered tracheae into regular clinical use.
20
Borthwick, Duncan William. "An investigation of murine trachea cellular kinetics : implications for stem cell gene therapy." Thesis, University of Edinburgh, 1998. http://hdl.handle.net/1842/21636.
Повний текст джерелаАнотація:
Submucosal glands were found to the major site of CFTR expression in the mouse trachea (an observation similar to that previously reported in humans). Reduced CFTR expression in this region may have the consequence of raising chloride concentrations in the airway surface fluid. In this thesis, data is presented showing that both the human and mouse homologue of the β-defensin-1 peptide shows a selective salt dependent antimicrobial activity. This may in part explain the higher susceptibility to colonisation by certain bacteria in the CF lung. In the search for possible progenitor cell populations in the mouse trachea, studies were conducted into the development of the submucosal glands and the kinetics of the tracheal epithelium in steady state and after damage. Through the study of aggregation chimaeric mice, evidence is presented to suggest that mouse submucosal glands are clonally derived from a single progenitor cell. In the mouse tracheal epithelium, basal cells were found to be the epithelial cell type with greatest proliferative capacity. Furthermore, a discrete niche of cells exhibiting stem cell characteristics were found in the submucosal gland ciliated duct. These cells were observed to be proliferative in times of stress and damage to produce migrating transit amplifying cells which repopulate and maintain the local epithelium. Experiments were conducted using liposome mediated gene therapy to approach the issue of targeting the tracheal progenitor cell populations. Although positive results for tissue transfection in vivo and in utero were obtained, the levels were disappointingly low. This thesis concludes that firstly, the mouse provides a useful animal model in which to study human tracheal kinetics and secondly, cells of high proliferative capacity displaying stem cell characteristics exist in the tracheal epithelium. The targeting of these progenitor cells may be of considerable importance in the future development of CF gene therapy.
21
Amendola, Richard Lee. "Graph-based segmentation of the pediatric trachea in MR images to model growth." Thesis, University of Iowa, 2012. https://ir.uiowa.edu/etd/2810.
Повний текст джерелаАнотація:
The upper airways are a major site of pediatric airway obstruction with its accompanying morbidity and mortality. The simplest approach to provide a stable airway is to perform a tracheotomy but it is a long recovery with its own complications. Other surgical procedures to reconstruct the airway require significant experience. The long-term objectives of this project are to develop a greater understanding of congenital abnormalities of the larynx and trachea. The objective of this thesis is to create a process to automatically segment and measure the pediatric trachea from MR images. Using 3DSlicer and ITK and program was created to perform the measurements. The software tool was optimized to produce similar results to that of CT image measurements from Pulmonary Workstation. The program was tested on a pediatric population and showed a significant correlation between cross-sectional area and age or height of the individual.
22
Negosanti, Luca <1981>. "Allotrapianto di trachea: studio anatomico sull'utilizzo del lembo ALT nell'allestimento del trapianto composito." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2016. http://amsdottorato.unibo.it/7703/1/negosanti_luca_tesi.pdf.
Повний текст джерелаАнотація:
La chirurgia della trachea è sempre stata una sfida per il chirurgo in considerazione delle notevoli difficoltà nel ripristinare la anatomia e la funzione dell’organo.
Attualmente il trapianto di trachea umano rivascolarizzato non è stato ancora realizzato, principalmente a causa della mancanza di un peduncolo vascolare tracheale noto.
Nel 2008 l’otorinolaringoiatra belga Paul Delaere ha descritto una tecnica di trapianto di trachea da cadavere mediante il confezionamento di un lembo composito.
Il lembo antibrachiale utilizzato è una ottima alternativa, ma oggi sono più utilizzati i lembi perforanti rispetto ai lembi assiali, in base a una serie di vantaggi ben noti. La possibilità di utilizzare il lembo perforante anterolaterale di coscia al posto del lembo “cinese” come base per il trapianto composito sfrutta i vantaggi di questo tipo di lembo.
Nello studio è stata valutata la fattibilità tecnica del lembo composito mediante una valutazione anatomica su cadavere.Trachea surgery has always been a challenge for the surgeon in view of remarkable difficulty in restoring the anatomy and the function. Currently revascularized human trachea transplantation is not possible due to the lack of a single vascular pedicle. In 2008 Paul Delaere described a tracheal transplantation technique using a cadaveric donor. The chinese flap used by Delaere to harvest the compiste flap is a good alternative, but today perforator flaps represent a better choice. The possibility to use the anterolateral thigh perforator flap in place of the chinese flap was studied on cadavers in the present stduy.
23
Negosanti, Luca <1981>. "Allotrapianto di trachea: studio anatomico sull'utilizzo del lembo ALT nell'allestimento del trapianto composito." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2016. http://amsdottorato.unibo.it/7703/.
Повний текст джерелаАнотація:
La chirurgia della trachea è sempre stata una sfida per il chirurgo in considerazione delle notevoli difficoltà nel ripristinare la anatomia e la funzione dell’organo.
Attualmente il trapianto di trachea umano rivascolarizzato non è stato ancora realizzato, principalmente a causa della mancanza di un peduncolo vascolare tracheale noto.
Nel 2008 l’otorinolaringoiatra belga Paul Delaere ha descritto una tecnica di trapianto di trachea da cadavere mediante il confezionamento di un lembo composito.
Il lembo antibrachiale utilizzato è una ottima alternativa, ma oggi sono più utilizzati i lembi perforanti rispetto ai lembi assiali, in base a una serie di vantaggi ben noti. La possibilità di utilizzare il lembo perforante anterolaterale di coscia al posto del lembo “cinese” come base per il trapianto composito sfrutta i vantaggi di questo tipo di lembo.
Nello studio è stata valutata la fattibilità tecnica del lembo composito mediante una valutazione anatomica su cadavere.Trachea surgery has always been a challenge for the surgeon in view of remarkable difficulty in restoring the anatomy and the function. Currently revascularized human trachea transplantation is not possible due to the lack of a single vascular pedicle. In 2008 Paul Delaere described a tracheal transplantation technique using a cadaveric donor. The chinese flap used by Delaere to harvest the compiste flap is a good alternative, but today perforator flaps represent a better choice. The possibility to use the anterolateral thigh perforator flap in place of the chinese flap was studied on cadavers in the present stduy.
24
Roberts, Andrew T. "Aerosol delivery of mammalian cells for tissue engineering." Link to electronic thesis, 2003. http://www.wpi.edu/Pubs/ETD/Available/etd-0429103-192655.
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25
Boers, James Evan. "Composition and proliferation of normal human tracheobronchial mucosa." Maastricht : Maastricht : Datawyse/Universitaire Pers Maastricht ; University Library, Maastricht University [Host], 1997. http://arno.unimaas.nl/show.cgi?fid=5926.
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26
Tonning, Anna. "Genetic and molecular regulation of epithelial tube morphogenesis /." Göteborg : Institute of Biomedicine, Sahlgrenska Academy, Göteborg University, 2006. http://hdl.handle.net/2077/704.
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27
Belchior, Luciana Dias. "Estudo das alteraÃÃes da contratilidade de anÃis de traquÃia isolada de ratos em resposta ao carbacol e ao potÃssio apÃs treinamento fÃsico." Universidade Federal do CearÃ, 2010. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=5151.
Повний текст джерелаАнотація:
FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgicoA presente pesquisa visou determinar a contratilidade do mÃsculo liso traqueal de ratos submetidos a exercÃcio leve de curto prazo, ou seja, treinados 1h ou prÃ-treinados e posteriormente exercitados por 1, 5 e 10 hs (PT1, PT5 e PT10, respectivamente), bem como de animais sensibilizados com OVA, submetidos ao exercÃcio por 5hs e posteriormente desafiados, com o objetivo de verificar as alteraÃÃes causadas pelo exercÃcio neste mÃsculo determinando tambÃm a interferÃncia da broncoprovocaÃÃo antigÃnica sobre a contratilidade traqueal de animais previamente exercitados. Para a realizaÃÃo da pesquisa, ratos machos (250-350g) foram submetidos a diferentes protocolos de nado, por 1, 5 e 10hs, sendo um grupo submetido à sensibilizaÃÃo com OVA e, 21 dias depois, desafiados em intervalos de 15 minutos atravÃs da inalaÃÃo do antÃgeno sensibilizante (grupo S/D). No grupo sensibilizado e submetido ao exercÃcio, este Ãltimo foi realizado durante 5 dias antes do desafio (grupo S/E prà desafio). O sacrifÃcio dos animais ocorreu 30 min apÃs a sessÃo de nado ou 24 horas apÃs o desafio antigÃnico no caso dos animais submetidos ao modelo de asma. Os ratos sedentÃrios nÃo foram submetidos a nenhum treino. Os controles do grupo asma inalaram apenas o veÃculo (NaCl 0,9%). Para os experimentos in vitro a traquÃia foi removida e montada em cuba para ÃrgÃo isolado contendo 5ml de soluÃÃo Tyrod (mantida a 37  0,5 ÂC) e aerada com mistura de O2. Foram confeccionadas curvas concentraÃÃo-efeito (CCE) para carbacol e cloreto de potÃssio. Os resultados mostraram que o pD2 nÃo apresentou valores significativos nos grupos em estudo, exceto em PT5 e PT10, ambos em relaÃÃo ao grupo PT1. A resposta mÃxima (%) apresentou valores significativamente crescentes e maiores nos grupos PT1 (107,62  3,57),PT5 (116,35  0,54) e PT10 (123,50  2,59) em relaÃÃo ao grupo sedentÃrio (82,70  5,90), quando o carbacol foi utilizado como estÃmulo contrÃtil. SituaÃÃo semelhante ocorreu nos grupos PT1 (105,19 Â1,12), PT5 (112,02  1,44) e PT10 (119,56  0,95) em relaÃÃo ao grupo sedentÃrio (90,45  2,22), para o potÃssio e nos grupos S/D (100,74  4,79) e S/E prÃ-desafio (109,09 Â3,68) em relaÃÃo ao grupo sensibilizado (82,69 Â5,90) para o carbacol, e nos grupos S/D (105,50  2,37) e S/E prÃ-desafio (115,05  1,96) em relaÃÃo ao grupo sensibilizado (93,11  3,58). Para verificar a participaÃÃo do Ãxido nÃtrico, acetilcolina e estresse oxidativo no mecanismo da aÃÃo prÃcontrÃtil da musculatura lisa traqueal induzida pelo exercÃcio foram determinados, respectivamente os nÃveis de nitrito, atividade da acetilcolinesterase (AChE) e espÃcies reativas ao Ãcido tiobarbitÃrico (TBARs) como uma maneira de determinar a peroxidaÃÃo lipÃdica. Os valores de nitrito foram significativos para o grupo PT5 em relaÃÃo aos grupos sedentÃrio e treinado 1h. Nos animais submetidos ao protocolo da asma ocorreu queda significativa dos nÃveis de nitrito nos grupos S/D e S/E prÃ-desafio. Ocorreu aumento da peroxidaÃÃo lipÃdica nos grupos PT1, PT5 e PT10 em relaÃÃo ao grupo sedentÃrio. Jà no grupo asmÃtico, os valores foram significativamente maiores nos grupos S/D e S/E prÃdesafio em relaÃÃo ao grupo controle. A atividade da AChE apresentou reduÃÃo significativa nos grupos PT1, PT5 e PT10 em relaÃÃo aos grupos sedentÃrio e treinado 1h. No grupo asmÃtico, tambÃm ocorreu reduÃÃo na atividade da AChE nos grupos S/D e S/E prÃ-desafio. Portanto, os resultados mostram que o exercÃcio a curto prazo aumenta a contratilidade da musculatura lisa traqueal isolada em ratos, para o carbacol e o potÃssio dos grupos em estudo; provavelmente com a participaÃÃo do Ãxido nÃtrico, acetilcolina e peroxidaÃÃo lipÃdica neste mecanismo prÃ-contrÃtil.
This work aimed to determine the contractility of tracheal smooth muscle of rats submitted to short-term light exercise, trained for 1h or pre-trained and, then, trained for 1, 5 and 10hs (PT1, PT5 and PT10, respectively), as well as, animals sensitized with OVA, submitted to exercise for 5hs and subsequently challenged, with the objective of verifying the changes caused by exercise in this muscle, also determining the interference of antigen bronchoprovocation on the contractility of tracheal smooth muscle of animals previously exercised. To conduct the study male rats (250-350g) were subjected to different protocols of swimming, by 1, 5 and 10hs, in addition to one group that was subjected to sensitization with OVA and 21 days later, challenged at intervals of 30 min through sensitizing antigen inhalation (group S/D). In the sensitized group submitted to exercise, this latter was held for 5 days before OVA challenge (group S/E pre-challenge). The animals were sacrificed 15 min after the swimming session or 24 hs after antigen challenge, in the case of rats submitted to asthma model. Sedentary rats were not subjected to any training session. Asthma control group inhaled only the vehicle (NaCl 0.9%). For in vitro experiments the trachea was removed and mounted in an isolated organ bath containing 5ml of Tyrode solution (maintained at 37 Â 0.5 ÂC) and aerated with O2. Concentration-effect curves (CEC) were designed for carbachol and potassium chloride. The results showed that the pD2 values were not significant among the groups except in PT5 and PT10, both in relation to group PT1. The maximum response values were significantly increased and higher in the groups PT1 (107,62 Â 3,57), PT5 (116,35 Â 0,54) and PT10 (123,50 Â 2,59) compared to the sedentary group (82.70 Â 5,90), when carbachol was used as the contractile stimulus. A similar situation occurred in the groups PT1 (105,19 Â 1,12), PT5 (112,02 Â 1,44) and PT10 (119,56 Â 0,95) as compared to the sedentary group (90,45 Â 2,22), for potassium and S/D (100,74 Â 4,79) and S/E pre-challenge groups (109,09 Â 3,68) as compared to the sensibilized group (82,69 Â 5,90) for carbachol, as well as, S/D (105,50 Â 2,37) and S/E pre-challenge (115,05 Â 1,96) groups when compared to the sensibilized one for potassium (93,11 Â 3,58). To verify the involvement of nitric oxide, acetylcholine, and oxidative stress on the pro-contractile mechanism of the tracheal smooth muscle induced by exercise, nitrite levels, acetylcholinesterase (AChE) activity and thiobarbituric acid reactive species (TBARS), as a way of determining lipid peroxidation, were determined. Nitrite levels decreased in the PT5 group as compared to sedentary and 1h trained groups. In the animals subjected to the asthma protocol there was a significant decrease in the nitrite levels in the S/D and S/E prechallenge groups. An increase in lipid peroxidation occurred in the groups PT1, PT5 and PT10 in relation to the sedentary one. In the asthmatic group, the TBARS values were significantly higher in S/D and S/E pre-challenge groups as compared to the control one. The AChE activity showed a significant reduction in the groups PT1, PT5 and PT10 in relation to sedentary and 1h trained groups. The groups S/D and S/E pre-challenge also presented a decrease on AChE activity. Therefore, the results showed that short-term light exercise increases the tracheal smooth muscle contractility of the study groups, probably with the participation of nitric oxide, acetylcholine, and lipid peroxidation in this pro-contractile mechanism.
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Geers, Teresa Anne. "The effect of subinhibitory concentrations of antibiotics on Pseudomonas aeruginosa infection /." The Ohio State University, 1986. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487266011222273.
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Liu, Yu. "Immunosuppressant-free allotrasplantation of the trachea : The antigenicity of tracheal grafts can be reduced by removing the epithelium and mixed glands from the graft by detergent treatment." Kyoto University, 2000. http://hdl.handle.net/2433/151405.
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Sands, Joyce Ann 1958. "THE INCIDENCE OF PULMONARY ASPIRATION IN INTUBATED PATIENTS RECEIVING ENTERAL NUTRITION THROUGH WIDE- AND NARROW-BORE NASOGASTRIC FEEDING TUBES." Thesis, The University of Arizona, 1986. http://hdl.handle.net/10150/276693.
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Tabcheh, Lina. "Tracheal mineralization : cellular and molecular mechanisms in mice." Thesis, Université de Lorraine, 2014. http://www.theses.fr/2014LORR0239/document.
Повний текст джерелаАнотація:
La trachée est une structure très complexe des voies respiratoires, qui est composée d'anneaux cartilagineux, fait de cartilage hyalin, et de bandes musculaires, formées de cellules musculaires lisses, dont l'architecture confère à la fois rigidité et souplesse au canal trachéen. Contrairement à d'autres cartilages, tels que ceux trouvés dans la plaque de croissance en développement et dans les articulations adultes, ou aux cellules musculaires lisses des vaisseaux, très peu d'informations sont disponibles sur le développement du cartilage et du tissu musculaire trachéal et sur leur capacité à se minéraliser, bien que la calcification de la trachée soit un événement commun dans la population âgée et plus rare dans certaines pathologies. Dans ce contexte, ce travail de thèse a cherché dans le modèle souris à mieux caractériser le cartilage et le tissu musculaire lisse de la trachée et également comprendre les mécanismes moléculaires jusqu'alors inexplorés, régulant la minéralisation de la trachée. Grâce à une nouvelle technique de culture de cellules provenant de la trachée, nous avons démontré que les chondrocytes et les cellules musculaires lisses trachéaux sont tous deux capables de minéraliser lorsqu'ils sont traités avec un haut niveau de Pi, mais via des mécanismes moléculaires différents. En parallèle, une étude in vivo nous a permis de démontrer que la minéralisation de la trachée se produit uniquement dans les anneaux cartilagineux dès 30 jours après la naissance. Des analyses histologiques et moléculaires ont permis d'affiner ces résultats et de proposer un modèle de minéralisation de la trachée via une progression rostro-caudale dépendante de BMP2The trachea is a very complex structure of the respiratory tract, composed of C-shaped cartilaginous rings, made of hyaline cartilage, and muscular bands, made of smooth muscle cells, conferring rigidity and compliance to the windpipe, respectively. In contrast to other intensely studied cartilages such as the ones found in the developing growth plate and in the adult joints or smooth muscle cells from the vasculature, very little information is available on the development of the tracheal cartilage and smooth muscle tissues and on their innate propensity to mineralize, although calcification of the trachea is a common finding in the elderly population and also a rare manifestation of pathologic conditions. In this context, this PhD work sought to better characterized the poorly studied tracheal cartilage and smooth muscle tissue and understand the molecular mechanisms regulating tracheal mineralization that has been unexplored so far. We tackle these questions in the mouse model. Setting up a novel in-vitro culture of tracheal cells, we demonstrated that tracheal chondrocytes and smooth muscles cells are prone to mineralize when treated with high level of Pi, through different molecular mechanisms. In parallel, we found that in vivo mineralization of the trachea only happens in the cartilaginous rings, as early as 30 days after birth. Histological and molecular evidence suggest that tracheal mineralization occurs through a BMP-dependent rostro-caudal progression
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Günther, Catherine Pascale [Verfasser]. "Untersuchungen zur mikrobiellen Besiedlung von Rachen und Trachea bei Bartagamen (Pogona spp.) / Catherine Pascale Günther." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2013. http://d-nb.info/1046709941/34.
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Salehi, Mehran. "Experimental Assessment of the Laryngeal Jet Effect on the Fluid Flow Pattern within the Trachea." University of Toledo / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1470386999.
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Tsuji, Takuya. "Long-term preservation of planar cell polarity in reversed tracheal epithelium." Kyoto University, 2018. http://hdl.handle.net/2433/232475.
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Loan, Lori A. "The relationship between ventilator inspired gas temperature and tracheal injury in neonates /." Thesis, Connect to this title online; UW restricted, 2000. http://hdl.handle.net/1773/7316.
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Schulte, Dagmar [Verfasser]. "Der Einfluss von Tumornekrosefaktor-alpha auf den ziliären Transport in der Trachea der Maus / Dagmar Schulte." Gießen : Universitätsbibliothek, 2015. http://d-nb.info/1069065366/34.
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Braxton, Joi Requan. "Effect of preload on the response of mouse trachea smooth muscle to cholinergic stimulation a thesis /." San Antonio : UTHSC, 2008. http://learningobjects.library.uthscsa.edu/cdm4/item_viewer.php?CISOROOT=/theses&CISOPTR=33&CISOBOX=1&REC=10.
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Marcus, Hilda. "Adherence of Pseudomonas aeruginosa to perfused tracheal epithelium : adhesin [i.e. adhesion] - receptor interactions /." The Ohio State University, 1985. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487259580261697.
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Marquardt, Claudio David [Verfasser]. "Funktionsuntersuchungen zum Einfluß von Temperatur auf den ziliären Transport in der Trachea von Mäusen / Claudio David Marquardt." Gießen : Universitätsbibliothek, 2015. http://d-nb.info/1078220271/34.
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Shibeshi, Alemayehu Workineh. "Etablierung und Charakterisierung primärer equiner Trachealepithelzellen: Ein in vitro-Modell zur Untersuchung der Expression und Funktion pulmonaler beta-adrenerger Rezeptoren." Doctoral thesis, Universitätsbibliothek Leipzig, 2010. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-38013.
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Die Kultivierung equiner Trachealepithelzellen stellt ein nützliches Modell dar, die (patho)-physiologischen Mechanismen der obstruktiven Atemwegserkrankungen des Pferdes auf zellulärer Ebene zu untersuchen. Ziel dieser Arbeit war es, Methoden für die Isolation,
Charakterisierung und weitergehende Kultivierung equiner Trachealepithelzellen (ETEZ) zu etablieren und validieren und die Expression und Funktionalität der beta-adrenergen Rezeptoren
an frisch isolierten ETEZ und deren Primärkulturen mittels pharmakologischer und biochemischer Verfahrenstechniken zu analysieren. Epithelzellen wurden durch Trypsinverdau aus der Trachea gesunder Pferde gewonnen, indem zuerst die Mukosa der
Trachea freigelegt und diese dann vom daruntergelegenen Bindegewebe stumpf getrennt wurde. Das gewonnene Gewebe wurde zerkleinert und enzymatisch mit 0,25% Trypsin-EDTA-Lösung für 2 h bei 37°C verdaut. Durch Siebung und Zentrifugation wurden die Zellen
gereinigt, vereinzelt und gesammelt, wobei kontaminierende Fibroblasten später durch differentielle Adhäsion von den Epithelzellen getrennt wurden. Die isolierten Zellen wurden sowohl licht- bzw. elektronenmikroskopisch charakterisiert, als auch immunzytochemisch
hinsichtlich Zytokeratin (für Epithelzellen) und Vimentin (für Fibroblasten) gefärbt. Die durchschnittliche Zellausbeute wurde mit der Neubauer-Zählkammer bestimmt und betrug 6,10 ± 0,63×106 Zellen pro 500 mg zerkleinertem Gewebe (n = 11). Die Zellvitalität wurde
mittels Trypanblau-Färbung ermittelt und betrug 94,70 ± 1,17% (n = 11).
Immunfluoreszensfärbungen zeigten, dass 93,57 ± 1,67% (n = 11) der frisch isolierten Zellen und ca. 100% (n = 5) der Primärkulturen auf Zytokeratin 5/6/18 positiv reagierten. Auf Anti-Vimentin reagierten dagegen nur 9,83 ± 0,94% (n = 11) der Zellen positiv. Die Zellen wurden in einer Dichte von 6,90 x 104 Zellen/cm2 in serumfreiem AECGM ausgesät und bildeten innerhalb einer Woche einen konfluenten Monolayer. Die konfluenten Zellen wurden mittels Dispase II abgelöst. Die erste (P1) und die zweite (P2) Passage konnte erfolgreich in serumfreien AECGM kultiviert und auf der Stufe P2 30 Tage lang gehalten werden. Weitethin wurden die Expression und Funktionalität der b-adrenergen Rezeptoren in frisch isolierten und kultivierten Epithelzellen untersucht. Mittels Radioligandenbindungsstudien, Westernblot, Immunfluoreszensfärbung und cAMP-Assays konnten erstmalig die Dichte, Affinität, Subtypen, Proteinexpression und zelluläre Lokalisation der beta-adrenergen Rezeptoren sowie die Rezeptorfunktion bestimmt werden. Messungen an frisch isolierten ETEZ ergaben für die mittlere Dissoziationskonstante (KD) von 31,78 ± 6,57 pM (n = 7) und eine maximale b-adrenerge Rezeptordichte (BMax) von 12727 ± 883,6 Bindungsstellen/Zelle (n = 7) ermittelt aus Sättigungsexperimenten mit dem b-adrenergen Rezeptorantagonisten [125I] Iodocyanopindolol (ICYP) in Anwesenheit des nicht selektiven beta-Rezeptorantagonisten
(±)-CGP 12177. Für Primärkulturen ergaben sich Werte für KD von 15,26 ± 3,37 pM (n =6) und für BMax von 3730 ± 212 Bindungsstellen/Zelle (n = 6). Bei Verdrängungsexperimenten wurde die ICYP konzentrationsabhängig durch den beta2-selektiven Rezeptorantagonisten ICI 118.551 und den beta1-selektiven Rezeptorantagonisten CGP 20712A verdrängt, wobei für ICI 118.551 eine 10.000-fach höhere Affinität (Ki = 1,74 ± 0,15 nM in frisch-isolierten Zellen und 1,19 ± 0,41 nM in Primärkultur) gezeigt wurde als für CGP 20712A (Ki = 17 ± 7,90 μM in frisch isolierten Zellen). Die cAMP-Bildung wurde in frisch isolierten ETEZ konzentrationsabhängig durch die beta-adrenergen Rezeptoragonisten Isoproterenol, Epinephrin und Norepinephrin in der Reihenfolge ihrer Potenz mit einer EC50 von 58 nM (n = 6), 13,60 μM (n = 6) bzw. 0,43 mM (n = 6) stimuliert. Diese cAMP-Bildung konnte durch Behandlung der Zellen mit 100 nM der beta2-selektiven ICI 118.551, nicht aber durch 300 nM des beta1-selektiven CGP 20712A blockiert werden. Mit einem beta2-adrenergen Rezeptorantikörper konnte eine 72 kDa Proteinbande und mit demselben Antikörper in der Fluoreszenzfärbung Rezeptorantigene auf der Zelloberfläche nachgewiesen werden. Zusammenfassend konnten mit dem etablierten Protokoll große Mengen equiner Trachealepithelzellen isoliert und kultiviert werden. Die Ergebnisse dieser Studie zeigen erstmalig, dass primäre equine Trachealepithelzellen funktionale beta2-adrenerge Rezeptoren exprimieren und das Protokoll zur Etablierung eines zellbasierten Modells geeignet ist, um in vitro verschiedene Funktionen und
eine Pharmaka-induzierte Regulation der beta-adrenergen Signalkaskade hinsichtlich physiologischer und pathophysiologischer Zustände bei Atemwegserkrankungen des Pferdes und hierfür relevante pharmakologische und toxikologische Zielstrukturen untersuchen zu können.
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Roethlisberger-Holm, Karin. "Transtracheal pressure recordings in the exercising horse /." Uppsala : Sveriges lantbruksuniv, 1995. http://epsilon.slu.se/avh/1995/91-576-4956-1.gif.
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Amoako, Daniel Kwasi. "Cyclopiazonic acid changes the mode of excitation-contraction couplingin acetylcholine-stimulated bovine tracheal smooth muscle." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1996. http://hub.hku.hk/bib/B3121387X.
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Servin, Silvio Oscar Noguera. "Tubo endotraqueal atraumático para ventilação mecânica." [s.n.], 2011. http://repositorio.unicamp.br/jspui/handle/REPOSIP/313790.
Повний текст джерелаАнотація:
Orientador: Alfio Jose TincaniTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciências Mèdicas
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Resumo: Introdução: Pacientes que necessitam permanecer sob intubação endotraqueal por longos períodos ou que são submetidos à anestesia geral, poderão ter lesão na luz da traquéia devido a pressões exercidas pelo balonete terminal do tubo endotraqueal (TET). Em alguns casos, estas lesões no epitélio poderão evoluir para estenose ou ocasionalmente necrose. Objetivo: Apresentar um modelo de tubo endotraqueal modificado (TETM) em que a pressão do balonete é variável com o ciclo da ventilação mecânica, sendo o mesmo avaliado em simulador pulmonar e animal. Método: Em simulador pulmonar acoplado a ventilador mecânico (VM) ajustado para dois volumes correntes (VC) de 10 e 15 mL/Kg e complacência de 60 mL/cmH²0, foram utilizados dois modelos de tubos endotraqueais para avaliar a eficiência da ventilação do TETM. O primeiro, identificado como modificado (TETM), e o segundo como convencional (TETC) ambos de número 7,5 mm e 8,0 mm. Foi ainda realizado comparação entre os dois modelos de tubos em suinos da raça Large White sob anestesia geral e VM por período de 48 horas contínuas, sendo os animais posteriormente eutanasiados para análise histopatológica das traquéias. Resultados: Ambos TETM (7,5 e 8,0 mm) apresentaram escape de ar no simulador pulmonar. O menor escape (13%) foi observado no TETM de 7,5 mm com VC = 15 mL/Kg e o maior escape (32%) no TETM de 8,0 mm com VC = 10 mL/Kg. Apesar disto, ambos TETM apresentaram boa eficiência no simulador pulmonar. Na avaliação do uso dos TET em porcos, a análise histopatológica dos cortes seriados de suas traquéias foi observado que o TETM causou menos áreas traumáticas em seu epitélio tanto macro como microscopicamente, quando comparado com o TETC. Conclusão: O uso de novo modelo de TET poderá diminuir o risco da ocorrência de lesão traqueal sem prejuízo relevante para a mecânica respiratória
Abstract: Introduction: Patients who need to undergo endotracheal intubation for a long periods or who are under general anesthesia, may have damage in the trachea light due to pressure from the cuff end. In some cases, the lesions in the tracheal epithelium may progress to stenosis or occasionally necrosis. Objective: Show a modified endotracheal tube (TETM) in both a lung simulator and animals in which the cuff pressure is variable according to the mechanical ventilation cycle. Method: In a lung simulator coupled to a mechanical ventilator (MV) fitted with two tidal volumes (Vt) (10 and 15mL/Kg) and compliance of 60mL/cmH20, two types of endotracheal tubes were used: modified (TETM) and conventional (TETC) with numbers 7.5 and 8.0 in order to evaluate the efficiency of TETM ventilation. There was also a comparison between two pipe models in MV anesthetized pigs for a period of 48 hours after which the animals were put down and a histopathology of the tracheas was carried out. Results: Both TETMs (7.5 and 8.0) had air leaks in the lung simulator. The smallest air leak (13%) was observed at 7.5 with TETM VC = 15 mL / kg and the highest (32%) at 8.0 with TETM VC = 10 mL / kg. In spite of that, both TETM showed good efficiency at lung simulator. From histopathological evaluation, in serial sections of the animals' trachea, we observed that TETM caused less traumatic areas in their epithelium, both macro and microscopically, when compared to TETC. Conclusion: The use of this new TETM may decrease the risk of occurrence of tracheal injury without relevant damage in respiratory mechanics
Doutorado
Fisiopatologia Cirúrgica
Doutor em Ciências da Cirurgia
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Nasr, Talia S. "Identification of Hedgehog/Gli Targets during Tracheoesophageal Development." University of Cincinnati / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1593273349807685.
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Haberman, Adam. "The role of trachealess and serrate in the development of the salivary glands and trachea of drosophila melanogaster." Available to US Hopkins community, 2003. http://wwwlib.umi.com/dissertations/dlnow/308067.
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Graulich, Tilman [Verfasser]. "Stereologische Untersuchungen der Gas-Austauschregion der Lunge und der Innervation der Trachea bei der tumorkachektischen Maus / Tilman Graulich." Gießen : Universitätsbibliothek, 2017. http://d-nb.info/1140734997/34.
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Soares, Semyramis Maria Freire [UNESP]. "Comparação do uso do tubo traqueal com balonete preenchido com ar, solução fisiológica ou lidocaína alcalinizada a 1% e a 0,5% em pacientes pediátricos." Universidade Estadual Paulista (UNESP), 2013. http://hdl.handle.net/11449/100137.
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soares_smf_dr_botfm.pdf: 880552 bytes, checksum: 62410b338ee94ef382aa18f6ceb2dce9 (MD5)A intubação traqueal de pacientes pediátricos, especialmente de crianças com idade inferior a oito anos, foi por muito tempo realizada exclusivamente com uso de tubos traqueais sem balonete devido ao risco do mesmo causar lesão na mucosa traqueal. Diversos estudos demonstraram a possibilidade do uso de cânulas traqueais com balonete de alta complacência e baixa pressão em crianças sem ter ocorrido aumento do risco de lesões de via aérea. O emprego da lidocaína alcalinizada no preenchimento do balonete apresentou diminuição na incidência de morbidade laringotraqueal no pós-operatório em adultos. Contudo, não há estudos comparando o uso de balonete preenchido com lidocaína alcalinizada e seus benefícios na população pediátrica. Comparar a utilização de cânulas traqueais com balonetes preenchidos com solução fisiológica, ar ou lidocaína alcalinizada a 1% e a 0,5% na prevenção de morbidades laringotraqueais, da agitação e da alteração hemodinâmica, no período pós-operatório, em pacientes com idade de três a 13 anos submetidos à anestesia geral. Cento e sessenta e quatro pacientes pediátricos submetidos à anestesia geral balanceada foram distribuídos em quatro grupos, de acordo com o preenchimento do balonete do tubo traqueal: grupo SF (n 41) preenchido com solução fisiológica 0,9%; grupo AR (n= 41) preenchido com ar ambiente; grupo L1% (n=41) preenchido com lidocaína alcalinizada a 1%; grupo L 0,5% (n=41) preenchido com lidocaína alcalinizada a 0,5%. Os balonetes foram lubrificados com gel hidrossolúvel antes da intubação traqueal e, após a intubação traqueal, preenchidos com ar ou a solução de acordo com o grupo até ser obtida a pressão de selo. A pressão do balonete foi mantida abaixo ou igual a 20 cm H2O. Os dados relativos aos atributos hemodinâmicos...
Tracheal intubation in pediatric patients, especially children under 8 years old, was for long performed exclusively with uncuffed tracheal tubes due to the risk of tracheal mucosal damage. Several studies have demonstrated the possibility of the use of cuffed tracheal tube with high compliance and low pressure in children. Its use has occurred without increasing risk of airway injury. The use of alkalized lidocaine filling the cuff showed a decrease in the incidence of laryngotracheal morbidity in adults postoperatively. However, there are no studies comparing the use of cuff filled with alkalinized lidocaine and its benefits in the pediatric population. compare the use of cuffed tracheal tubes filled with saline, air or alkalized 1% and 0.5% lidocaine in preventing laryngotracheal morbidity, and hemodynamic changes in the postoperative period in patients aged between 3 and 13 years undergoing general anesthesia. one hundred sixty four pediatric patients undergoing balanced general anesthesia were divided into four groups, according to the filling of the tracheal tube cuff: saline group (n=41) filled with 0.9% saline; AR group (n=41) filled with ambient air; L1% group (n=14) filled with alkalinized 1% lidocaine; group L 0.5% (n=41) filled with alkalinized 0.5% lidocaine. All cuffs were lubricated with water-soluble gel before tracheal intubation. After tracheal intubation, they were filled until reaching sealing pressure. The cuff pressure was kept below or equal to 20 cm H2O. Data on respiratory and hemodynamic parameters were obtained before intubation and after 30, 60, 90 and 120 minutes of anesthesia and at the end of surgery, immediately before and after extubation. After 30 minutes of intubation, a sample of peripheral venous blood was collected from patients who had the cuffs filled with lidocaine for determination of its plasma concentration... (Complete abstract click electronic access below)
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Silva, Irene Raimundo dos Santos. "Influência dos corticosteróides nas propriedades biomecânicas da traquéia de coelhas albinas." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/82/82131/tde-04022005-155455/.
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Foram estudados os efeitos da metilprednisolona na traquéia de coelhas albinas adultas da raça Nova Zelândia. O objetivo deste estudo foi investigar os efeitos potenciais desta substância nas propriedades biomecânicas da traquéia de coelhas por meio de ensaio de tração axial. Os animais foram divididos em dois grupos. Dez coelhas do grupo controle receberam por via subcutânea 2 mg/Kg/dia de metilprednisolona diluída em solução salina. As dez coelhas do grupo controle receberam também de modo subcutâneo volumes proporcionais de solução salina. Após 21 dias, os animais foram sacrificados e suas traquéias submetidas a ensaio de tração e estudo histopatológico. Os animais do grupo experimental apresentaram peso significantemente inferior ao do grupo controle no final do tratamento (C = 3996 ± 314 g x E = 3555 ± 373 g). Os grupos não diferiram do ponto de vista estatístico quanto as seguintes variáveis: carga no limite de proporcionalidade (C = 8,94 ± 1,68 N x E = 8,93 ± 1,52 N); carga máxima (C = 9,12 ± 2,2 N x E = 9,57 ± 1,90 N); alongamento no limite de proporcionalidade (C = (683 ± 2,08) x '10 POT.-3' m x E = (6,05 ± 1,50) x '10 POT.-3' m); alongamento máximo (C = (7,44 ± 2,60) x '10 POT.-3' m x E = (6,85 ± 1,55) x '10 POT.-3' m); energia absorvida na fase elástica (C = (28,70 ± 14,43) x '10 POT.-3' J x E = (27,75 ± 11,06) x '10 POT.-3' J); rigidez (C = (1,08 ± 0,41) x '10 POT.-3' N/m x E = (1,2 ± 0,37) x '10 POT.-3' N/m). Concluiu-se ao término deste estudo que não houve alterações morfológicas e biomecânicas das traquéias estudadas, no entanto, os animais do grupo experimental sofreram significante emagrecimento ao final do tratamento, quando comparado ao grupo experimentalThe effects of methilprednisolona in the adults trachea albinos female rabbits were studied. The goal of this study was examine the potential effects of this armorial traction. The animals were divided in two groups. Ten female rabbits of control group received by way of subcutaneous 2mg/kg/day of salt bed diluted solution. These ten female rabbits of the control group also received by way of subcutaneous proportional volumes of salt bed solution. After 21 days, the animals were sacrificed and their trachea were subjected to traction tests and anatomical pathological studies. Animals of the experimental group presented inferior weight significant from the control group in the end of the treatment (C = 8,94 ± 314g X E = 3555 ± 373g). The groups didnt differ from the statistic point of view as regards of the following variables: limit load of proportionality (C = 8,94 ± 1,68 N X E = 8,93 ± 1,52 N); maxim load (C = 9,12 ± 2,2 N X E = 9,57 ± 1,90 N); stretch out on the limit of proportionality (C = (683 ± 2,08) x '10 POT.-3' m X E = (6,05 ± 1,50) x '10 POT.-3' m); maxim stretch out (C = (7,44 ± 2,60) x '10 POT.-3' J X E = (6,85 ± 1,55) x '10 POT.-3' J); absorbed energy in the elastic phase (C = 28,70 ± 14,43) x '10 POT.-3' m J X E = (27,75 ± 11,06) x '10 POT.-3' J); rigidity (C = 1,08 ± 0,41) x '10 POT.-3' N/m X E = (1,2 + 0,37) x '10 POT.-3' N/m). It was concluded in the end of the treatment that there werent any morphological and biomechanical alterations of the trachea studied, although, the animals of the experimental group suffered a significant slimming in the end of the treatment when compared with the experimental group
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Evaristo, Thaiane Cristine [UNESP]. "Avaliação histológica e funcional do enxerto de neotraqueia de coelho desenvolvido por bioengenharia." Universidade Estadual Paulista (UNESP), 2015. http://hdl.handle.net/11449/139360.
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000860334.pdf: 6389809 bytes, checksum: 30c9e72299bc6974c576c11090b390a0 (MD5)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Tendo em vista o desafio do tratamento de injúrias traqueais e o progresso na produção desse órgão por técnicas de engenharia de tecidos, faz com que o interesse nessa área cresça de forma considerável, já que ainda não há um tratamento padrão descrito na literatura acerca das lesões traqueais que acometem 50% do comprimento total do órgão de um adulto ou 30% da traqueia de uma criança. Assim, este estudo tem como objetivo a construção de modelo de traqueia para enxerto utilizando técnicas de engenharia de tecidos. Para tanto, foram realizados cinco e 10 ciclos de descelularização nas traqueias de coelhos doadores seguindo etapas: congelamento/descongelamento sem adição de nenhum crioprotetor, banho ultrassônico no aparelho Ultrasonic cleaner (Unique model USC 1400®) na frequência de 40 Khz durante 10 minutos, irradiação utilizando diodo de emissão de luz (LED) azul com comprimento de onda de 450nm ± 20nm na dose de 90 J/cm2 totalizando 60 minutos de exposição, adição do detergente deoxicolato de sódio a 4% sob agitação a 180 rpm no C24 Incubator shaker (New Brunswick Scientific) durante 48 horas. Paralelamente, foram realizadas expansão e caracterização das células-tronco mesenquimais dos coelhos receptores com posterior diferenciação dessas células em condrócitos e células de músculo liso. Finalmente, foi realizada a recelularização das traqueias com aplicação das células-tronco mesenquimais, dos condrócitos e das células de músculo liso dos coelhos receptores nas superfícies externas dos arcabouços produzidos para os transplantes traqueais. Após cinco ciclos repetidos do protocolo de descelularização utilizado, ainda é possível observar células epiteliais respiratórias e condrócitos remanescentes nas traqueias dos coelhos doadores. Porém, a repetição de 10 ciclos do mesmo protocolo de descelularização remove completamente as células epiteliais respiratórias e reduz a...
There is no standard treatment yet described in the literature for tracheal lesions whose length is more than 50% in adults and a third of the trachea in small children. Given the challenge of tracheal injuries treatment and the organ generation progress by tissue engineering techniques the interest in this area is growing considerably. Thereby this study aims to produce tracheal model for graft using tissue engineering techniques. Were performed five and 10 cycles of decellularization on donor rabbit‟s tracheae following the steps: freezing/defreezing without addition of any cryoprotectant; sonication in Ultrasonic cleaner (Unique model USC 1400®) with 40 Khz for 10 minutes; blue light-emitting diode (LED) irradiation with wavelength of 450nm ± 20nm along a dose of 90 J/cm2 at a total exposure of 60 minutes; addition of sodium deoxicholate detergent 4% during 48 hours under 180 rpm agitation in the C24 Incubator Shaker (New Brunswick Scientific). Meanwhile, expansion and characterization of mesenchymal stem cells of rabbits' receptors were performed with subsequent differentiation of these cells into chondrocytes and smooth muscle cells. Finally, it was realized the tracheal recellularization by the application of the mesenchymal stem cells, chondrocytes and smooth muscle cells from receptors rabbits in the outer face of the produced scaffolds for tracheal transplantation. After five repeated cycles of decellularization protocol used, it is still possible to observe respiratory epithelial cells and chondrocytes remaining in the tracheae of the donor rabbits. However, after 10 cycles of the same decellularization protocol the respiratory epithelial cells are completely removed and the tracheal cartilage chondrocytes are reduced. Animals that have been transplanted with five cycles decellularized tracheae had died after 10-12 days due to rejection by the presence of donor rabbit‟s epithelial cells. Animals that have been ...
FAPESP: 2010/50155-2
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Evaristo, Thaiane Cristine. "Avaliação histológica e funcional do enxerto de neotraqueia de coelho desenvolvido por bioengenharia /." Botucatu, 2015. http://hdl.handle.net/11449/139360.
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Orientador: Daniela Cristina CataneoCoorientador: Eleneice Deffune
Banca: Francisco Guerreiro Cardoso
Banca: Flávia Cilene Maciel da Cruz Alves
Banca: Luiz Felippe Júdice
Banca: Érica Nishida Hasimoto
Resumo: Tendo em vista o desafio do tratamento de injúrias traqueais e o progresso na produção desse órgão por técnicas de engenharia de tecidos, faz com que o interesse nessa área cresça de forma considerável, já que ainda não há um tratamento padrão descrito na literatura acerca das lesões traqueais que acometem 50% do comprimento total do órgão de um adulto ou 30% da traqueia de uma criança. Assim, este estudo tem como objetivo a construção de modelo de traqueia para enxerto utilizando técnicas de engenharia de tecidos. Para tanto, foram realizados cinco e 10 ciclos de descelularização nas traqueias de coelhos doadores seguindo etapas: congelamento/descongelamento sem adição de nenhum crioprotetor, banho ultrassônico no aparelho Ultrasonic cleaner (Unique model USC 1400®) na frequência de 40 Khz durante 10 minutos, irradiação utilizando diodo de emissão de luz (LED) azul com comprimento de onda de 450nm ± 20nm na dose de 90 J/cm2 totalizando 60 minutos de exposição, adição do detergente deoxicolato de sódio a 4% sob agitação a 180 rpm no C24 Incubator shaker (New Brunswick Scientific) durante 48 horas. Paralelamente, foram realizadas expansão e caracterização das células-tronco mesenquimais dos coelhos receptores com posterior diferenciação dessas células em condrócitos e células de músculo liso. Finalmente, foi realizada a recelularização das traqueias com aplicação das células-tronco mesenquimais, dos condrócitos e das células de músculo liso dos coelhos receptores nas superfícies externas dos arcabouços produzidos para os transplantes traqueais. Após cinco ciclos repetidos do protocolo de descelularização utilizado, ainda é possível observar células epiteliais respiratórias e condrócitos remanescentes nas traqueias dos coelhos doadores. Porém, a repetição de 10 ciclos do mesmo protocolo de descelularização remove completamente as células epiteliais respiratórias e reduz a...
Abstract: There is no standard treatment yet described in the literature for tracheal lesions whose length is more than 50% in adults and a third of the trachea in small children. Given the challenge of tracheal injuries treatment and the organ generation progress by tissue engineering techniques the interest in this area is growing considerably. Thereby this study aims to produce tracheal model for graft using tissue engineering techniques. Were performed five and 10 cycles of decellularization on donor rabbit‟s tracheae following the steps: freezing/defreezing without addition of any cryoprotectant; sonication in Ultrasonic cleaner (Unique model USC 1400®) with 40 Khz for 10 minutes; blue light-emitting diode (LED) irradiation with wavelength of 450nm ± 20nm along a dose of 90 J/cm2 at a total exposure of 60 minutes; addition of sodium deoxicholate detergent 4% during 48 hours under 180 rpm agitation in the C24 Incubator Shaker (New Brunswick Scientific). Meanwhile, expansion and characterization of mesenchymal stem cells of rabbits' receptors were performed with subsequent differentiation of these cells into chondrocytes and smooth muscle cells. Finally, it was realized the tracheal recellularization by the application of the mesenchymal stem cells, chondrocytes and smooth muscle cells from receptors rabbits in the outer face of the produced scaffolds for tracheal transplantation. After five repeated cycles of decellularization protocol used, it is still possible to observe respiratory epithelial cells and chondrocytes remaining in the tracheae of the donor rabbits. However, after 10 cycles of the same decellularization protocol the respiratory epithelial cells are completely removed and the tracheal cartilage chondrocytes are reduced. Animals that have been transplanted with five cycles decellularized tracheae had died after 10-12 days due to rejection by the presence of donor rabbit‟s epithelial cells. Animals that have been ...
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