Дисертації з теми "Toxin A and Toxin B"
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Fernandes, da Costa Sérgio Paulo. "Molecular and structural characterisation of epsilon toxin and necrotic enteritis toxin B : two pore-forming toxins from Clostridium perfringens." Thesis, University of Exeter, 2013. http://hdl.handle.net/10871/14608.
Повний текст джерелаBalmforth, Matthew Royce. "Piggybacking on the cholera toxin : using cholera toxin B chain for the targeted delivery of proteins to motor neurones." Thesis, University of Leeds, 2017. http://etheses.whiterose.ac.uk/20115/.
Повний текст джерелаFlagler, Michael J. "Determination of the Molecular Basis for the Difference in Potency between Shiga Toxins 1 and 2." Cincinnati, Ohio : University of Cincinnati, 2010. http://rave.ohiolink.edu/etdc/view.cgi?acc_num=ucin1267131436.
Повний текст джерелаAdvisor: Alison A. Weiss. Title from electronic thesis title page (viewed Apr. 26, 2010). Keywords: Shiga toxin; Shiga-like toxin; Verotoxin; Verocytotoxin; B-subunit; B-pentamer. Includes abstract. Includes bibliographical references.
檀東煇 and T. F. Tan. "Elucidation of ganglioside binding domain in the B-subunit of cholera toxin." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2000. http://hub.hku.hk/bib/B31223448.
Повний текст джерелаTan, T. F. "Elucidation of ganglioside binding domain in the B-subunit of cholera toxin." Hong Kong : University of Hong Kong, 2000. http://sunzi.lib.hku.hk/hkuto/record.jsp?B23636634.
Повний текст джерелаBatisse, Cornélie. "Targeting strategies using B-subunit of Shiga toxin : innovative drug-delivery systems." Thesis, Sorbonne Paris Cité, 2015. http://www.theses.fr/2015USPCB220.
Повний текст джерелаWe need new therapeutic strategies to treat cancerous patients by the discovery of new drugs that would be more active than those existing and especially assigning fewer side effects. These new therapies aim to specifically target cancer cells. Among the strategies for cancer targeting, we investigated drug-targeted strategies using a proteic carrier, STxB, derived from Shiga toxin. This protein recognizes specifically its biological receptor Gb3, which is over-expressed on human cancer cells. This work consisted in the design and synthesis of conjugates combining STxB and a cytotoxic drug. The chemical linker binding these two moieties was carefully designed in order to fit requirements of both stability and ability to trigger a drug-delivery. A first linker was designed around a mercaptoethanol core, able to be conjugated to STxB by a disulfide bond. This constitutes a drug-delivery trigger, activated by a biological reducing agent such as glutathion, and followed by a self-immolative step. Two highly potent conjugates of auristatin derivatives were obtained and showed promising results in vitro. The ester bonds lability in acidic pH was exploited for the design of two amino acid based linker. With the aim of increasing the ratio of drug on STxB, we investigated several multivalent linkers. Another option was to consider gold nanorods as a nanometric platform, able to carry thousands of drugs and STxB. The incorporation of a protease substrate to produce an enzyme-cleavable linker was investigated. Several spacers, which induced release of the drug under native form or under prodrug form, were designed and tested
Lipscombe, Martin John. "Construction and characterisation of Escherichia coli heat-labile toxin B-subunit fusion proteins." Thesis, University of Warwick, 1991. http://wrap.warwick.ac.uk/108070/.
Повний текст джерелаSchneider, Olivia Dawn. "An Analysis of the Effects of Pertussis Toxin on T Cell Signaling." University of Cincinnati / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1258667926.
Повний текст джерелаPéré-Védrenne, Christelle. "Etude de la Cytolethal Distending Toxin B des Hélicobacters dans l’inflammation et la carcinogenèse digestive." Thesis, Bordeaux, 2015. http://www.theses.fr/2015BORD0404/document.
Повний текст джерелаThe demonstration of the role of the Cytolethal Distending Toxin (CDT) of Helicobacter hepaticusin the development of hepatocarcinoma in mice, makes this toxin a relevant candidate in theactivation of precancerous processes. As in the case of the CagA toxin of Helicobacter pylori, theCdtB active subunit of CDT could be an oncoprotein. We studied the role of Helicobacter CdtB ininflammation and digestive carcinogenesis using a lentiviral strategy for constitutive or conditionalexpression of the CdtB subunit or its corresponding DNase mutant. We conducted a study of thetranscriptome and showed that CdtB induced an inflammatory response by overexpressingcytokines, chemokines, antimicrobial peptides and activating the NF-kB pathway in epithelialcells. The CdtB also regulated the expression and nuclear localization of the transcription factorand oncogene MafB. These results were confirmed for the CdtB of Helicobacter pullorum.Infection of cells with wild type strains and the corresponding CDT-mutant strains (of H. hepaticus& H. pullorum) were used to validate the results and to attribute the effects to the CdtB and, inparticular, to its DNase activity. We also developed a novel epithelial cell xenograft model toevaluate the inducible expression of H. hepaticus CdtB. In this model, the CdtB, in addition to itspreviously well-known effects, delayed tumor growth, induced apoptosis, senescence and theoverexpression of nuclear proliferation marker, Ki-67, suggesting cell survival. All of these resultsprovide new arguments in favor of the oncogenic potential of the CDT
Kalluri, Anila. "EXPRESSION OF CHOLERA TOXIN B SUBUNIT-ROTAVIRUS NSP4 ENTEROTOXIN FUSION PROTEIN IN TRANSGENIC CHLOROPLASTS." Master's thesis, University of Central Florida, 2005. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/3069.
Повний текст джерелаM.S.
Department of Molecular Biology and Microbiology
Burnett College of Biomedical Sciences
Molecular Biology and Microbiology
Maisey, Elizabeth Anne. "Botulinum toxin types A and B : isolation and biological characterisation of their polypeptide chains." Thesis, Imperial College London, 1990. http://hdl.handle.net/10044/1/46426.
Повний текст джерелаEswaran, Jeyanthy. "Purification and characterisation of recombinant C. perfringens beta toxin from E. coli and B. subtilis." Thesis, University of Newcastle Upon Tyne, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.341444.
Повний текст джерелаMurarasu, Thomas. "The Shiga Toxin B-Subunit : a Promising Scaffold for the Targeting of Tumor Specific Glycosphingolipids." Thesis, Université Paris-Saclay (ComUE), 2017. http://www.theses.fr/2017SACLS512.
Повний текст джерелаCancer is the second cause of death worldwide. Recent advance in cancer treatments involved the identification of cancer biomarkers and the development of efficient therapeutic products able to specifically recognize them. This new class of products has the ability to specifically target tumor cells, with the major advantages to decrease or abolish treatments side effects and relapses of the disease. Unfortunately, a certain number of patients do not respond to those treatments lacking the expression of those biomarkers on their tumor. This project aims at developing new targeted therapies by exploiting a new class of cancer biomarkers, which would potentially extend the therapeutics options against cancer
Johnson, Nicholas. "Construction of a novel epitope expression vector based on the B-subunit of the diphtheria toxin." Thesis, University of Southampton, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296057.
Повний текст джерелаMartin, Daniel Dalton. "Purification of Anthrax Toxin Protective Antigen Component and Characterization of its Binding Interaction with Bovine Kidney Cells." DigitalCommons@USU, 1986. https://digitalcommons.usu.edu/etd/4641.
Повний текст джерелаRosselot, Andrew E. "Ontogeny of the intestinal circadian clock and its role in the response to Clostridium difficile toxin B." University of Cincinnati / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1573222475839068.
Повний текст джерелаThorsell, Mikaela. "Evaluation of C. diff Quik Chek Complete® and comparison with GeneXpert to establish a new diagnostic algorithm." Thesis, Uppsala universitet, Institutionen för kvinnors och barns hälsa, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-390609.
Повний текст джерелаAdam-Castrillo, David. "Local Administration of Botulinum Toxin Type-B in the External Anal Sphincter of Horses Produces Transient Reduction of Peak Anal Pressure." Thesis, Virginia Tech, 2003. http://hdl.handle.net/10919/33927.
Повний текст джерелаMaster of Science
Gomez, Sheena Robin. "Investigation of pertussis toxin A- and B-subunit activities in acellular vaccines by enzymatic and carbohydrate-binding assays." Thesis, University of Glasgow, 2007. http://theses.gla.ac.uk/40959/.
Повний текст джерелаGhei, M. "Effects of Botulinum toxin B on refractory detrusor overactivity : a randomised, double-blind, placebo controlled, cross over trial." Thesis, University College London (University of London), 2008. http://discovery.ucl.ac.uk/1444297/.
Повний текст джерелаRahn, Sarah Jane. "Allergy models and related assays to test the allergic qualities of Escherichia coli heat labile toxin subunit B." [Ames, Iowa : Iowa State University], 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:1468126.
Повний текст джерелаAyache, Alexandra. "Amyloid-beta42 toxicity reduction in human neuroblastoma cells using cholera toxin b subunit-myelin basic protein expressed in chloroplasts." Honors in the Major Thesis, University of Central Florida, 2012. http://digital.library.ucf.edu/cdm/ref/collection/ETH/id/1535.
Повний текст джерелаID: 031908400; System requirements: World Wide Web browser and PDF reader.; Mode of access: World Wide Web.; Accepted in partial fulfillment of the requirements for honors in the major in DEPT HERE.; Thesis (B.A.)--University of Central Florida, 2012.; Includes bibliographical references.
B.S.
Bachelors
Burnett School of Biomedical Sciences
Molecular Biology and Microbiology
Aman, Abu Tholib. "Mutagenesis of a conserved loop in the B subunit of cholera toxin : identification of residues essential for toxicity and immunomodulation." Thesis, University of Bristol, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.311431.
Повний текст джерелаDugyala, Raviprakash R. "Alteration of Key Cytokine Levels by Aflatoxin B1 and T-2 Toxin in Male CD-1 Mice." DigitalCommons@USU, 1995. https://digitalcommons.usu.edu/etd/4653.
Повний текст джерелаNityanandam, Ramya. "Expression and functional evaluation of exendin 4 fused to cholera toxin B subunit in tobacco chloroplast to treat type 2 diabetes." Master's thesis, University of Central Florida, 2011. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/4815.
Повний текст джерелаID: 031001317; System requirements: World Wide Web browser and PDF reader.; Mode of access: World Wide Web.; Title from PDF title page (viewed March 26, 2013).; Thesis (M.S.)--University of Central Florida, 2011.; Includes bibliographical references (p. 35-40).
M.S.
Masters
Molecular Biology and Micro
Medicine
Biotechnology
Bast, Darrin James. "Three biologically significant globotriasylceramide binding sites on the Verotoxin 1 B subunit, implications in toxin action, pathogenesis of disease and vaccine design." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ35106.pdf.
Повний текст джерелаAndo, Yoshikazu. "Inactivation of Rho GTPases with Clostridium difficile toxin B impairs centrosomal activation of Aurora-A in G2/M transition of HeLa cells." Kyoto University, 2008. http://hdl.handle.net/2433/135856.
Повний текст джерелаAliprandini, Eduardo. "Obtenção de anticorpos monoclonais humanos antitetânicos." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-04122015-141425/.
Повний текст джерелаMonoclonal antibodies (mAbs) for therapeutic use correspond to a major area of the biopharmaceutical industry, especially human mAbs that are less prone to elicit immunogenicity. The objective of this work was to obtain anti-tetanus human mAbs through separation of memory B lymphocytes producing specific antibodies stained with the antigen or plasmablasts. Cells were collected from peripheral blood of donors after vaccination and separated through cell sorting. The variable regions of the antibodies were amplified and cloned in expression vectors for transient transfection of HEK293-F cells. The staining with the tetanus toxin labeled independently with two markers, biotin and Alexa Fluor® 647 allowed the separation of specific B lymphocytes producing anti-tetanus mAbs. The antibodies expressed were evaluated by ELISA, western blotting and the inhibition of the binding of the tetanus toxin to the ganglioside GT1b. The in vivo neutralization assay showed that a pool of three different mAbs were able to protect mice against the tetanus toxin.
Fühner, Viola [Verfasser], Michael [Akademischer Betreuer] Hust, and Stefan [Akademischer Betreuer] Dübel. "Development of neutralizing and non-neutralizing antibodies targeting known and novel epitopes on Clostridioides difficile Toxin B / Viola Fühner ; Michael Hust, Stefan Dübel." Braunschweig : Technische Universität Braunschweig, 2020. http://d-nb.info/1203299036/34.
Повний текст джерелаKapzan, Ruth [Verfasser], Gernot [Akademischer Betreuer] Längst, and Ralf [Akademischer Betreuer] Wagner. "Generierung neuer HIV-1 Impfstoffkandidaten zur Induktion breit neutralisierender Antikörper mit Cholera Toxin B als Trägerprotein / Ruth Kapzan. Betreuer: Gernot Längst ; Ralf Wagner." Regensburg : Universitätsbibliothek Regensburg, 2012. http://d-nb.info/1024198928/34.
Повний текст джерелаFraser, Sylvia A. "The role of GM1-binding in mediating the immunomodulatory properties of the B subunits of cholera toxin and Escherichia coli heat-labile enterotoxin." Thesis, University of Bristol, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.391162.
Повний текст джерелаFrazão, Renata. "Análise citoarquitetônica e imunoistoquímica de estruturas do sistema visual de macacos-prego (Cebus apella)." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/42/42131/tde-08092008-105313/.
Повний текст джерелаThe diurnal habits and its complex SNC, make the tufted capuchin monkey an important subject for the study of the visual system. In the present study, five tufted capuchins received a single intraocular neuronal tracer subunit B of cholera toxin (CTb) injection and perfused 15 days later. The retina and brain were removed from the animals and processed with immunohistochemical techniques. The CTb analysis showed that the retina send projections to several structures, such as primary visual, optical accessory and circadian control systems. The immunohistochemical characterization also showed two different types of bipolar cells in the retina. These cells, differently from other species, were co-localized with gabaergic receptors. Overall our results showed several interspecies differences suggesting that comparison of the visual system between species must be undertaken with great caution.
Nascimento, Dilzamar Veloso do. "Construção, clonagem e expressão do fragmento B da toxina diftérica de Corynebacterium diphtheriae (cepa PW- 8) em Mycobacterium bovis BCG sub-cepa Moreau." Universidade do Estado do Rio de Janeiro, 2014. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=9023.
Повний текст джерелаA vacina anti-diftérica de uso corrente no Brasil (DTP), embora de alta eficácia na prevenção da difteria, está associada com episódios de toxicidade e reatogenicidade no recipiente vacinal, resultantes de proteínas residuais derivadas do processo de produção ou detoxificação. Estratégias para o desenvolvimento de vacinas menos reatogênicas e ao mesmo tempo mais eficazes e economicamente viáveis contra a difteria têm sido alvo de intensa investigação. A alternativa proposta por nosso grupo é a utilização da vacina contra a tuberculose (Mycobacterium bovis BCG sub-cepa Moreau), como vetor do gene que codifica o fragmento B da toxina diftérica (dtb) de 58,3 kDa. Neste trabalho o dtb foi clonado no vetor micobacteriano bifuncional (pUS977) de expressão citoplasmática e os clones recombinantes (pUS977dtbPW8), após a transformação do BCG, foram testados com relação a expressão do DTB em BCG e quanto a antigenicidade frente a anticorpos policlonais anti-toxóide diftérico por Immunobloting. A integridade do gene dtb e a identidade das sequências de DNA da construção plasmidial pUS977dtbPW8 foram confirmadas por sequenciamento de DNA e análise de similaridade. A imunogenicidade do BCGr pUS977dtbPW8 expressando o DTB foi investigada em camundongos BALB/c, os resultados obtidos revelaram uma soroconversão específica (IgG). A infectividade e atividade microbicida do BCGr pUS977dtbPW8 no ambiente intracelular foi avaliada através da infecção de linhagens de células de monócitos humano (THP-1), os dados obtidos indicaram que houve sobrevivência intracelular em até 12 dias. Nesse contexto, esplenócitos dos camundongos imunizados com 30 e 60 dias foram extraídos, mostrando que o BCGr pUS977dtbPW8 persistiu até 60 dias na ausência de pressão seletiva e a viabilidade celular não sofreu alteração significativa durante o período testado. Por outro lado, o BCGr pUS977dtbPW8, quando submetido a seis sub-cultivos consecutivos in vitro não apresentou diferença significativa na capacidade de expressar o DTB, demonstrando portanto a persistência da estabilidade funcional da linhagem recombinante. A estabilidade estrutural da construção pUS977dtbPW8 também foi avaliada por PCR confirmando a presença do gene dtb em colônias do BCGr pUS977dtbPW8 . Adicionalmente, foi possível avaliar preliminarmente in vitro a capacidade soroneutralizante dos soros de camundongos imunizados com BCGr pUS977dtbPW8 após 30 e 60 dias em células VERO. A ação citotóxica da toxina diftérica entre as diluições de 1/4 e 1/16 foram neutralizadas com o pool de soros imunes com 60 dias. Finalmente, em nosso estudo foi possível avaliar o potencial da vacina BCG como vetor de expressão de um antígeno de Corynebacterium diphtheriae in vitro e in vivo.
The diphtheria vaccine currently used in Brazil (DTP), despite its history of high efficacy in the prevention of diphtheria, is associated with episodes of toxicity and vaccine reactogenicity in the vaccinee, resulting from the presence in the vaccine of residual proteins derived from the production process or detoxification. Strategies for the development of new vaccines more effective and economically viable against diphtheria have been the subject of intense investigation. The alternative proposed by our group is the use of the vaccine against tuberculosis (Mycobacterium bovis BCG Moreau sub strain) as a vector for the gene that encodes the 58.3 kDa fragment B of the diphtheria toxin (DTB). In our project the dtb gene was cloned into the bifunctional vector pUS977 for cytoplasmic expression and recombinant BCG (rBCG) clones, selected after transformation of BCG, were tested for expression of the DTB polypeptide and antigenicity against polyclonal antibodies anti- diphtheria toxoid by immunoblotting. The integrity and identity of the DNA sequence encoding the dtb gene carried by the plasmid construct pUS977dtbPW8 was confirmed by DNA Sequencing and Analysis of Similarity. The immunogenicity of the rBCG expressing the DTB was investigated in BALB/c mice and the results revealed a specific seroconversion (IgG). Also, infectivity and microbicidal activity were analyzed in the intracellular environment by infecting human monocytes (THP-1 cell line) with rBCG. The data obtained indicated intracellular survival within 12 days. In this context, splenocytes collected from mice at days 30 and 60 after immunization were removed and assayed for live bacteria. The results showed that rBCG persisted viable up to 60 days in the absence of selective pressure and cell viable counts did not change significantly during testing. Additionally, the rBCG subjected to six consecutive sub-cultures in vitro showed no significant difference in the ability to express the DTB, thus demonstrating the functional stability of the recombinant vaccine. The structural stability of the construct pUS977dtbPW8 was also confirmed by PCR detection of the dtb gene in rBCG colonies. Also, it was possible to have a preliminary evaluation of the neutralizing capacity of sera from mice immunized with BCGr 30 and 60 days after immunization. The cytotoxic action of diphtheria toxin, between dilutions 1/ 4 and 1/16, was neutralized by mice sera in an in vitro assay using VERO cells. Finally, in our study it was possible to evaluate the potential of BCG as a vector for expression of an antigen of Corynebacterium diphtheriae in vitro and in vivo.
Beyer, April Jean. "Evaluation of low dose exposure and immunogenicity of transgenic maize expressing the Escherichia coli heat-labile toxin B subunit when fed intermittently and daily." [Ames, Iowa : Iowa State University], 2007.
Знайти повний текст джерелаKrabs, Isabel [Verfasser]. "Untersuchungen zur Suppression der NF-κB-Aktivierung [NF-kappa-B-Aktivierung] in Shiga-Toxin-produzierenden Escherichia coli (STEC) infizierten Säugerzellen / vorgelegt von Isabel Krabs". Gießen : DVG-Service, 2007. http://d-nb.info/988696908/34.
Повний текст джерелаOng, Kong Wee. "Effects of a bacterial toxin on LMP specific CTL killing of EBV transformed B cells : the effect on systemic inflammatory response and clinical outcome." Thesis, University of Bristol, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271912.
Повний текст джерелаFélix, Mellanie Karoline do Carmo. "Antígeno inativado de Clostridium Novyi tipo B em emulsão W/O: uma prova de conceito em camundongos Swiss visando o controle de necrose hepática de ruminantes." Universidade Federal do Tocantins, 2018. http://hdl.handle.net/11612/965.
Повний текст джерелаBrazilian cattle breeding has great emphasis on the national market. Diseases that affect herds compromise the market as well as generate great economic losses. Clostridium novyi type B causes hepatic necrosis in cattle through the production of alpha toxin, a potent exotoxin that reduces productivity through changes such as hemoglobinuria, reduced appetite, fever, lethargy, decreased milk and stool production. Containing the causative micro-organism becomes a necessary quest both economically and socially. However, control of the disease is still performed by vaccines formulated with multiple antigens. The emulsion may be a promising alternative for the improvement of antigen adsorption in vaccine formulations. Swiss strain mice were used to evaluate clinical aspects and validate results regarding the composition of a new vaccine formulation containing Montanide ISA 61 VG adjuvant and C. novyi inactivated antigen. Characterization and antigenicity tests indicated the presence of the alpha toxin protein in the evaluated composition. The immunogenicity of antigen inactivated in W / O emulsion (water / oil) was verified and the ratio employed (40/60) showed to be ideal in the use of multiple antigens, presenting innocuousness, product stability, controlled release and stimulation of the immune response. The determination of the antigen concentration was investigated by the active antigen ratio and inactivated with sera from sick animals, since the vaccine efficacy was 40%. The suitability of the inactivated alpha toxin concentration in the emulsion was shown to be necessary to achieve better animal protection values. Hemogram, biochemical and liver, spleen and thigh morphology contributed to elucidate the effects of the emulsion and to verify hepatic necrosis in the nonimmunized groups, in addition to suggesting advances in the adsorption of vaccines. The results allowed the establishment of a murine model of C. novyi infection with future applications related to the vaccine production with multiple emulsified antigens to control clostridia.
Chahboun, Siham. "Comparaison des régions variables des anticorps de macaques (Macaca fascicularis) et de l' Homme et leurs utilisation pour la neutralisation des toxines botuliques A et B." Thesis, Grenoble, 2013. http://www.theses.fr/2013GRENV022.
Повний текст джерелаOur laboratory has developed a strategy to isolate recombinant antibody fragments technology from immunized non human primates (Macaca fascicularis) by phage display. In the course of the present thesis, a comparison between macaque (Macaca mulatta) and human antibody sequences has demonstrated that antibodies of the two species are different. This difference makes the humanization of macaque antibodies desirable. The strategy was used in the framework of the European AntiBotABE project, and the screening was adapted to isolate antibody fragments cross neutralizing the B1 and B2 subtypes of botulinum B neurotoxin, by using sequentially the holotoxin BoNT/B1 and a recombinant fragment representing the C-terminal region of the heavy chain of BoNTB2. The best scFv targeting the C-terminal region of BoNT/B1 and BoNTB2 heavy chains, B2-7, demonstrated a high capacity to neutralize BoNT/B1 and BoNT/B2 in the ex vivo hemidiaphragmatic assay. A high identity (80%) between the framework regions of B2-7 and their human homologs was observed. ScFvs neutralizing BoNT/A1 by targeting its light chain were also isolated and among them, the scFv 2H8 induced a decrease of 50% in the endopeptidase activity at a concentration corresponding to a molar ratio of 2H8/BoNT/A1 of 64000. A high identity (88%) between the framework regions of 2H8 and their human homologs was also observed. Our strategy can be used to isolate other therapeutic antibody fragments
Moser, Rebekka. "IgG Antiköperbestimmung gegen Haemophilus influenza Typ b, Streptococcus pneumoniae Stereotypen 14 und 19F und Clostridium tetani Toxin in Nabelschnurseren und Seren von 0-6 Monate alten Säuglingen /." [S.l.] : [s.n.], 1998. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.
Повний текст джерелаPrice, Gregory A. "Immunogenicity of the Gonococcal Transferrin Binding Proteins." VCU Scholars Compass, 2005. http://scholarscompass.vcu.edu/etd_retro/76.
Повний текст джерелаLica, Marta Anna [Verfasser], and Harald [Akademischer Betreuer] Genth. "Unterschiede in der biologischen Wirkung des Clostridium difficile Toxin B in proliferierenden und nicht-proliferierenden Zellen / Marta Anna Lica. Institut für Toxikologie der Medizinischen Hochschule Hannover. Betreuer: Harald Genth." Hannover : Bibliothek der Medizinischen Hochschule Hannover, 2012. http://d-nb.info/1023136376/34.
Повний текст джерелаJacquier, Muriel. "Association covalente de C3b à la toxine tétanique : rôle dans l'apprêtement et la présentation de l'antigène aux lymphocytes T." Grenoble 1, 1992. http://www.theses.fr/1992GRE10200.
Повний текст джерелаHickey, Ashley N. "Expression of CTB-proinsulin in transgenic chloroplasts." Honors in the Major Thesis, University of Central Florida, 2008. http://digital.library.ucf.edu/cdm/ref/collection/ETH/id/1088.
Повний текст джерелаBachelors
Burnett College of Biomedical Sciences
Molecular and Microbiology
Silva, Sebasti?o Franco da. "Centros subcorticais dos sistemas visual prim?rio e ?ptico acess?rio no moc? (kerodon rupestris): caracteriza??o pela proje??o retiniana e citoarquitetura." Universidade Federal do Rio Grande do Norte, 2009. http://repositorio.ufrn.br:8080/jspui/handle/123456789/17208.
Повний текст джерелаConselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico
The primary and accessory optic systems comprise two set of retinorecipient neural clusters. In this study, these visual related centers in the rock cavy were evaluated by using the retinal innervations pattern and Nissl staining cytoarchigtecture. After unilateral intraocular injection of cholera toxin B subunit and immunohistochemical reaction of coronal and sagittal sections from the diencephalon and midbrain region of rock cavy. Three subcortical centres of primary visual system were identified, superior colliculus, lateral geniculate complex and pretectal complex. The lateral geniculate complex is formed by a series of nuclei receiving direct visual information from the retina, dorsal lateral geniculate nucleus, intergeniculate leaflet and ventral lateral geniculate nucleus. The pretectal complex is formed by series of pretectal nuclei, medial pretectal nucleus, olivary pretectal nucleus, posterior pretectal nucleus, nucleus of the optic tract and anterior pretectal nucleus. In the accessory optic system, retinal terminals were observed in the dorsal terminal, lateral terminal and medial terminal nuclei as well as in the interstitial nucleus of the superior fasciculus, posterior fibres. All retinorecipient nuclei received bilateral input, with a contralateral predominance. This is the first study of this nature in the rock cavy and the results are compared with the data obtained for other species. The investigation represents a contribution to the knowledge regarding the organization of visual optic systems in relation to the biology of species.
Os sistemas visual prim?rio e ?ptico acess?rio compreendem dois conjuntos de grupamentos neurais, que recebem proje??o direta da retina. Nesse estudo, estes dois sistemas foram avaliados com rela??o a citoarquitetura e padr?o de inerva??o retiniana, usando como tra?ador neural anter?grado, a subunidade B da toxina col?rica revelada por imunoistoqu?mica para detectar este tra?ador em terminais sobre grupamentos neuronais (alvos) no enc?falo do moc? (Kerodon rupestris), um roedor nativo do Nordeste Brasileiro. Os resultados permitiram identificar os componentes do sistema visual prim?rio o complexo geniculado lateral, o complexo pr?-tectal e o col?culo superior. O complexo geniculado lateral cont?m o n?cleo geniculado lateral dorsal, o n?cleo geniculado lateral ventral e o folheto intergeniculado. Todos recebem fibras da retina com predomin?ncia contralateral, menos intensa para o folheto intergeniculado. O complexo pr?-tectal cont?m os n?cleos pr?-tectal anterior, pr?-tectal medial, pr?-tectal posterior, olivar pr?-tectal e n?cleo do trato ?ptico, os quais recebem proje??o retiniana predominantemente contralateral. Do mesmo modo, o col?culo superior recebe fibras da retina contralateral nas camadas superficiais. Tamb?m foi identificado o sistema ?ptico acess?rio completo no moc?, constitu?do pelos n?cleos terminal medial, terminal lateral, terminal dorsal e intersticial do fasc?culo superior posterior. Esses n?cleos recebem inerva??o retiniana com forte predomin?ncia contralateral, sendo que o n?cleo terminal medial, embora preserve a predomin?ncia contralateral, exibe uma evidente inerva??o ipsolateral.
Kuk, Chiu Ying. "Anthrax Lethal Toxin Is a Tumor Hemorragic Toxin." Thesis, Van Andel Research Institute, 2018. http://pqdtopen.proquest.com/#viewpdf?dispub=10973827.
Повний текст джерелаBlood supply is crucial for tumor growth and metastasis. However, current anti-angiogenic therapy is not as effective as predicted, thus a better understanding of the tumor angiogenic process and new anti-angiogenic agent are urgently required. Anthrax lethal toxin (LeTx) has an anti-angiogenic effect on tumors. Tumors treated with LeTx are smaller, paler, and have lower mean vessel density compared to control treated tumors. Most interestingly, compared to current anti-angiogenic treatment, LeTx does not cause normalization of tumor vessels. Instead, tumors treated with LeTx have massive hemorrhages, pointing to a potential alternative mechanism to inhibit tumor angiogenesis. I hypothesize that instead of causing “normalization” of tumor vasculature, LeTx’s anti-angiogenic effects works in a manner similar to a hemorrhagic toxins. To test this hypothesis, I compared the effect of LeTx to snake venom metalloproteinase, a known hemorrhagic toxin, in tumor vasculature. Quantified by Nuance multispectral imaging system, both LeTx and SVMP caused an increase in tumor hemorrhage. Futher analysis of vasculature integrity using continued vessel length showed disruption of vessels by LeTx and SVMP. With these results, I conclude that the anti-angiogenic effects of LeTx are due to its hemorrhagic nature, and not due to normalization of tumor vasculature. Further understanding of LeTx mechanism can help design novel anti-angiogenic agent that compliments current therapy.
Javid-Khojasteh, Vahideh. "Toxic Shock Syndrome Toxin-1 : detection of the toxin, anti-toxin antibodies and producer organisms in a paediatric burns unit." Thesis, University of Salford, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.365993.
Повний текст джерелаBader, Carly. "The cytopathic activity of cholera toxin requires a threshold quantity of cytosolic toxin." Master's thesis, University of Central Florida, 2013. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/5762.
Повний текст джерелаM.S.
Masters
Molecular Biology and Microbiology
Medicine
Biomedical Sciences; Biomedical Sciences
Gao, Haifei. "Chemical biology approaches to study toxin clustering and lipids reorganization in Shiga toxin endocytosis." Thesis, Sorbonne Paris Cité, 2015. http://www.theses.fr/2015USPCB147.
Повний текст джерелаBacterial Shiga toxins bind to the glycosphingolipid (GSL) globotriaosylceramide (Gb3) to enter cells by clathrin-dependent and independent endocytosis. In the clathrin-independent pathway, Shiga toxin reorganizes membrane lipids in a way such as to impose mechanical strain onto the bilayer, thus leading to the formation of deep and narrow endocytic pits. Mechanistically how this occurs is not yet understood, and notably how the geometric properties of toxin-GSLs complexes translate into function has remained enigmatic. In my thesis work, using the B-subunit of Shiga toxin (STxB) as a model, different molecular species of its receptor Gb3 have been synthesized with deliberately chosen structures, coupled with high resolution imaging and computational modeling, to understand the underlying mechano-chemical constraints leading to efficient toxin clustering and lipids reorganization. By combining dissipative particle dynamics (DPD) computer simulation and experiments on cell and model membranes, we provided evidence that a membrane fluctuation-induced force, termed Casimir-like force, drives the aggregation of tightly membrane-associated toxin molecules at mesoscopic length scales. Furthermore, toxin-induced lipid condensation was observed and measured quantitatively on Langmuir monolayers using X-ray reflectivity (XR) and grazing incidence x-ray diffraction (GIXD), thereby providing direct evidence for the hypothesis that the toxin has the potential to asymmetrically reduce the molecular area of the exoplasmic membrane leaflet, leading to local membrane deformation. During my PhD, effort was also invested to develop new GSL tools applied to the biological setting. A novel strategy based on the Cu-free click reaction between glycosyl-cyclooctyne and azido-sphingosine was designed with the goal to functionally incorporate GSLs into cellular membranes. Following the synthesis work, click reactions have been performed in solution and on cells. Compared to the former, results on cells were far less efficient. Further optimization is currently ongoing. A fluorescently labeled Gb3 probe with Alexa Fluor 568 coupled via a PEG linker to the α-position of the acyl chain, was synthesized, to which STxB bound on TLCs, but not on model membranes. Further improvements are discussed
Karlsson, Sture. "Toxin production in Clostridium difficile /." Stockholm : Karolinska institutet, 2004. http://diss.kib.ki.se/2004/91-77349-812-2/.
Повний текст джерелаStrack, Julia [Verfasser], and Andreas [Akademischer Betreuer] Bechthold. "Osteoklastendifferenzierung durch Pasteurella multocida-Toxin." Freiburg : Universität, 2014. http://d-nb.info/1123480834/34.
Повний текст джерела