Дисертації з теми "Tissues Cryopreservation"
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Beaty, Myron H. "Cryopreservation of eukaryote algae." Thesis, This resource online, 1991. http://scholar.lib.vt.edu/theses/available/etd-07282008-135156/.
Повний текст джерелаVogel, Martin Joseph. "Proteomic profiling following cryopreservation." Diss., Online access via UMI:, 2004. http://wwwlib.umi.com/dissertations/fullcit/1424168.
Повний текст джерелаMukherjee, Indra Neil. "A rational design approach for the cryopreservation of natural and engineered tissues." Diss., Atlanta, Ga. : Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/22579.
Повний текст джерелаCommittee Chair: Sambanis, Athanassios; Committee Member: Long, Jr., Robert C.; Committee Member: Ludovice, Peter J.; Committee Member: Prausnitz, Mark R.; Committee Member: Song, Ying C.
Xu, Xia. "A study of mass transfer in cryopreservation of living tissues." Thesis, University of Oxford, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.275199.
Повний текст джерелаEls, Cecilia Lydia. "Early human follicle ultrastructure comparison after slow cryopreservation in two different cryoprotectants." Thesis, Link to the online version, 2008. http://hdl.handle.net/10019/831.
Повний текст джерелаHiggins, Adam Zachary. "Intracellular ice formation in tissue constructs and the effects of mass transport across the cell membrane." Diss., Atlanta, Ga. : Georgia Institute of Technology, 2007. http://hdl.handle.net/1853/28166.
Повний текст джерелаCommittee Chair: Karlsson, Jens; Committee Co-Chair: Nerem, Robert; Committee Member: Meda, Paolo; Committee Member: Prausnitz, Mark; Committee Member: Sands, Jeff; Committee Member: Zhu, Cheng.
Kagawa, Keiko Sompop Prathanturarug. "Cryopreservation of dendrobium cruentum Rchb. f. /." Abstract, 2006. http://mulinet3.li.mahidol.ac.th/thesis/2549/cd394/4738650.pdf.
Повний текст джерелаBedaiwy, Mohamed Ali. "Ovarian tissue cryopreservation and transplantation : approaches and techniques /." Cleveland, Ohio : Cleveland Clinic, 2007. http://www.loc.gov/catdir/toc/ecip082/2007042633.html.
Повний текст джерелаFuku, Eiji. "Studies on the cryopreservation of immature and in vitro matured bovine - oocytes." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41590.
Повний текст джерелаIn the first series of experiments, bovine oocytes were vitrified (V-oocytes) or frozen slowly (S-oocytes) at the germinal vesicle (GV) stage or after maturation in vitro (IVM). Survival was assessed morphologically and also by in vitro fertilization (IVF) and culture (IVC). Morphological integrity and developmental capacity were greater in S-oocytes than in V-oocytes (P $<$ 0.05). Transfer of four embryos (2 morulae and 2 blastocysts) derived from post-IVM S-oocytes into a recipient heifer resulted in the birth of twin calves.
In the second series of experiments, oocytes (GV and IVM) were exposed to a cryoprotectant solution (DAP213: 2M DMSO, 1M acetamide, 3M propanediol) for 1.5 or 5 min and viability assessed by IVM-IVF-IVC. Oocytes were also examined by transmission electron microscopy (TEM) before (control) or after exposure to the cryoprotectant. DAP213 induced profound premature cortical granule (CG) release and vesiculation. These changes were less pronounced in oocytes exposed to DAP213 only after IVM. The results suggest that: (1) the extrusion of CG is one of the important cytological events affected by the treatment of oocytes with DAP213; (2) GV oocytes are more sensitive to the cryoprotectant than IVM oocytes.
In the third series of experiments, GV and IVM oocytes were vitrified with DAP213. On rewarming, DAP213 was removed by a one- or three-step dilution procedure and survival assessed by development after IVM-IVF-IVC. Morphology was assessed by TEM study immediately following DAP213 removal. Both assessments indicated that: (1) IVM oocytes are more tolerant to vitrification than are GV oocytes; (2) the three-step dilution is less damaging than the one-step procedure; (3) changes in the zona pellucida (loss of plasticity) of IVM oocytes following vitrification may result from the premature release of cortical granules.
Lawson, Alison N. "Cryopreservation effects on the in vitro and in vivo function of a model pancreatic substitute." Diss., Georgia Institute of Technology, 2011. http://hdl.handle.net/1853/39540.
Повний текст джерелаStott, Shannon Leigh. "Kinetic Study of Intracellular Ice Formation in Micropatterned Endothelial Cell Cultures Using High Speed Video Cryomicroscopy." Diss., Georgia Institute of Technology, 2006. http://hdl.handle.net/1853/16256.
Повний текст джерелаBrossollet, Louis-Joseph. "Effects of cryopreservation on the biaxial mechanical properties of canine saphenous veins." Diss., Georgia Institute of Technology, 1992. http://hdl.handle.net/1853/19241.
Повний текст джерелаWebb, Veronica Fine Gross Guenter W. "Investigation of cryopreservation methods for adherent nerve cell networks in vitro." [Denton, Tex.] : University of North Texas, 2009. http://digital.library.unt.edu/ark:/67531/metadc12213.
Повний текст джерелаSanchez, Daniel Andres. "The effects of cryopreservation on the viscoelastic properties of the canine anterior cruciate ligament." Thesis, Georgia Institute of Technology, 1988. http://hdl.handle.net/1853/19321.
Повний текст джерелаSalinas-Flores, Liliana, and n/a. "Understanding and improving the cryopreservation of pacific oyster (Crassostrea gigas) oocytes via the use of two approaches : modification of an existing cryopreservation protocol and manipulation of the lipis fraction of the oocytes." University of Otago. Department of Food Science, 2008. http://adt.otago.ac.nz./public/adt-NZDU20080305.143446.
Повний текст джерелаBathgate, Roslyn. "Studies on the cryopreservation of boar spermatozoa and its integration into assisted reproductive technologies." Connect to full text, 2004. http://hdl.handle.net/2123/1279.
Повний текст джерелаTitle from title screen (viewed 13 January 2009). Degree awarded 2005; thesis submitted 2004. Submitted in fulfilment of the requirements for the degree of Doctor of Philosophy to the Faculty of Veterinary Science. Includes bibliographical references. Also available in print form.
Webb, Veronica Fine. "Investigation of cryopreservation methods for adherent nerve cell networks in vitro." Thesis, University of North Texas, 2009. https://digital.library.unt.edu/ark:/67531/metadc12213/.
Повний текст джерелаAhmad, Hajira Fatima. "Cryopreservation effects on a pancreatic substitute comprised of beta cells or recombinant myoblasts encapsulated in non-adhesive and adhesive alginate hydrogels." Diss., Georgia Institute of Technology, 2012. http://hdl.handle.net/1853/48968.
Повний текст джерелаLetsoalo, Phutiane Thomas. "Characterisation and cryopreservation of semen from indigenous Namaqua Afrikaner sheep breed, in comparison with Dorper and Dohne Merino breeds." Thesis, University of Fort Hare, 2017. http://hdl.handle.net/10353/4759.
Повний текст джерелаBlais, Louis. "The evaluation of spermatozoal damage done at each step of the cryopreservation procedure from a line of chicken selected for high fertility, of frozen-thawed semen and a random, bred control line /." Thesis, McGill University, 1988. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=61847.
Повний текст джерелаChan, Chun-wai. "In-vitro study of the cryopreserved intervertebral disc." Click to view the E-thesis via HKUTO, 2008. http://sunzi.lib.hku.hk/hkuto/record/B41290380.
Повний текст джерелаMaceda, Tintaya Edwin Eddy. "Effect of three cryoconservation diluents on sperm motility and vitality in the ejaculate of bulbourethal-ectomized llamas (Lama glama), department of La Paz." BYU ScholarsArchive, 2008. https://scholarsarchive.byu.edu/etd/5391.
Повний текст джерелаChan, Chun-wai, and 陳春慧. "In-vitro study of the cryopreserved intervertebral disc." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2008. http://hub.hku.hk/bib/B41290380.
Повний текст джерелаPustowka, Cory V. "Effects of source of dietary lipid on the fatty acid composition and cholesterol content of tissues, semen cryopreservation and embryo survival in rainbow trout (Oncorhynchus mykiss)." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ35463.pdf.
Повний текст джерелаYap, Cheng-Hon. "Factors influencing cryopreserved allograft heart valve degeneration." Connect to thesis, 2006. http://repository.unimelb.edu.au/10187/2120.
Повний текст джерелаSumpter, Megan Louise. "Johnson-Mehl-Avrami Kinetics of Intracellular Ice Formation in Confluent Tissue Constructs." Thesis, Georgia Institute of Technology, 2004. http://hdl.handle.net/1853/7263.
Повний текст джерелаFasano, Giovanna. "Contribution of vitrification to human assisted reproduction." Doctoral thesis, Universite Libre de Bruxelles, 2013. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/209484.
Повний текст джерелаMalgré le fait que la cryopréservation soit une technique très attractive, elle peut avoir des effets délétères sur les cellules. Les protocoles expérimentaux visent donc à minimiser ces effets afin d’augmenter la survie et la compétence cellulaire après décongélation.
Les deux méthodes les plus utilisées, la congélation lente et la vitrification, présentent chacune des avantages et des inconvénients. En effet, la première ne permet pas d’éliminer la cristallisation intracellulaire. Quant à la seconde, elle empêche la formation de cristaux de glace mais pourrait provoquer une toxicité due à la forte concentration des cryoprotecteurs.
Cette thèse de doctorat propose plusieurs objectifs :
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Doctorat en Sciences biomédicales et pharmaceutiques
info:eu-repo/semantics/nonPublished
Sipen, Philip. "Tissue Culture and Cryopreservation of Banana {Musa spp.)." Thesis, University of Nottingham, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.523611.
Повний текст джерелаTurner, Shane. "Cryopreservation of somatic germplasm of selected Australian monocotyledonous taxa (Haemodoraceae)." Thesis, Curtin University, 2001. http://hdl.handle.net/20.500.11937/1409.
Повний текст джерелаUnhale, Sanket Anil. "Cryobiology of Cell and Tissue Cryopreservation: Experimental and Theoretical Analysis." Diss., The University of Arizona, 2011. http://hdl.handle.net/10150/202974.
Повний текст джерелаTurner, Shane. "Cryopreservation of somatic germplasm of selected Australian monocotyledonous taxa (Haemodoraceae)." Curtin University of Technology, Department of Environmental Biology, 2001. http://espace.library.curtin.edu.au:80/R/?func=dbin-jump-full&object_id=12678.
Повний текст джерела0.8 M glycerol rather than sorbitol, (3) utilisation of PVS2 solutions with reduced DMSO content; and (4) incorporation of an additional loading phase (2 M glycerol plus 0.4 M sucrose for 20 mins at 0 degrees celsius).Macropidia fuliginosa, a species with poor recovery after LN exposure, was successfully cryostored using somatic embryos. Treatments which resulted in the highest survival (67.3% 5.7 plus or minus %) included preculture with 0.4 M sorbitol, and incubation in PVS2 for 30 min. Further experimentation indicated that preculture for two days on 0.8 M glycerol (replacing 0.4 M sorbitol) was more beneficial for achieving high post-LN survival.Post-LN survival was significantly correlated to the use of polyalcohols when the total number of hydroxyl (-OH) groups (regardless of molarity) present was the same as that found in 0.4 M sorbitol. It was hypothesised that hydroxyl number is more important than molarity in membrane stabilisation, during dehydration and cooling. Post-LN survival was also found to be significantly influenced by stereochemical arrangement of the -OH groups of polyalcohol molecules used in the preculture media. Finally, post-LN survival was also found to be significantly influenced by the size of the molecule, with smaller polyalcohols with more -OH groups on one flank of the carbon chain being superior as cryoprotective agents.The influence of plant growth regulators on post-LN survival and recovery growth was also investigated. The survival of shoot apices was not correlated to cytokinin or auxin treatments administered in culture media prior to cryostorage. However, in the recovery medium, a combination of cytokinin and 0.5 mu M GA(subscript)3 in the medium was found to be the most efficacious for obtaining healthy plantlets.Genetic fidelity was then examined using Amplified Fragment Length Polymorphism (AFLP). Plantlets of one done kept ++
or maintained under the following conditions: (1) standard tissue culture conditions; (2) cold storage and (3) cryostorage, over a 12 month duration, showed no detectable genetic changes.Further, shoot apex viability evaluated at regular intervals (after 0, 3, 6 and 12 months of LN storage) suggested that medium term storage of samples cryopreservation did not reduce shoot apex viability over this time span.This study has provided a better understanding of the factors influencing post-LN survival and recovery and, as a result, the cryopreservation protocols have been refined. Consequently, the prospects for conserving threatened Haemodoraceae species from Western Australia through cryostorage is now significantly improved.
Wiswedel, Klaus. "Sperm cryopreservation and artificial insemination at Groote Schuur Hospital." Master's thesis, University of Cape Town, 1987. http://hdl.handle.net/11427/25895.
Повний текст джерелаLiu, Jianan. "Cryopreservation and transplantation of ovarian tissue in Japanese quail (Coturnix japonica)." Thesis, University of British Columbia, 2009. http://hdl.handle.net/2429/17424.
Повний текст джерелаAl-Forkan, Mohammad. "Agrobacterium-mediated transformation of Indica rice (Oryza sativa L.)." Thesis, University of Nottingham, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342480.
Повний текст джерелаZhang, Pu. "Human ovarian follicles and oocytes : collection, cryopreservation, culture and gene expression /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-281-0/.
Повний текст джерелаCharles, Lara Nicole. "Culture of cells from mammalian tissue cryopreserved without cryoprotection." [College Station, Tex. : Texas A&M University, 2008. http://hdl.handle.net/1969.1/ETD-TAMU-2819.
Повний текст джерелаLiu, Jianan. "Cryopreservation and transplantation of gonadal tissue for genetic conservation and biological research in avian species." Thesis, University of British Columbia, 2013. http://hdl.handle.net/2429/45101.
Повний текст джерелаAnil, Siji. "Development of in-vitro culture and cryopreservation protocol for zebrafish (Danio rerio) ovarian tissue fragments." Thesis, University of Bedfordshire, 2013. http://hdl.handle.net/10547/308923.
Повний текст джерелаHuang, Haishui. "Microfluidic Generation and Manipulation of Hydrogel Microcapsules for Biomimetic 3D Tissue Culture and Cell Cryopreservation." The Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1461095928.
Повний текст джерелаKaczmarczyk, Anja. "Physiological, biochemical, histological and ultrastructural aspects of cryopreservation in meristematic tissue of potato shoot tips." Berlin Köster, 2008. http://d-nb.info/991906160/04.
Повний текст джерелаCarvalho, Allan Charles Marques de. "Criopreservação de sêmen de tambaqui Colossoma macropomum em criotubo." Pós-Graduação em Zootecnia, 2013. https://ri.ufs.br/handle/riufs/6391.
Повний текст джерелаA criopreservação de sêmen em criotubos reduz o tempo necessário para o envase, congelamento e descongelamento das amostras, além de otimizar os procedimentos de fertilização artificial. No entanto, nenhum estudo ainda foi realizado com o sêmen de tambaqui neste recipiente. Assim, o objetivo do presente trabalho foi avaliar a influência do tipo de criotubo (1,6 e 4,5 mL) e do tempo de descongelamento (60ºC/70s e 60ºC/90s) sobre a qualidade e fertilidade do sêmen de tambaqui criopreservado. Para isso, amostras de sêmen foram diluídas em solução de congelamento (1:9 v/v) composta por 75% de glicose 290 mOsm, 10% de metilglicol e 5% de gema de ovo, sendo envasadas em criotubos de 1,6 e 4,5 mL, congeladas em vapor de nitrogênio líquido no botijão dry-shipper (-175ºC) e armazenadas em botijão criogênico a -196°C. Para avaliação do tempo de descongelamento do sêmen, os criotubos foram imersas em água a 60°C durante 70 s ou 90 s e a qualidade espermática imediatamente avaliada (Motilidade total - MT; Motilidade progressiva - MP; Velocidade curvilinear - VCL; Velocidade em linha reta - VSL e Velocidade média da trajetória - VAP). Neste estudo foi determinado também o tempo de viabilidade dos espermatozoides descongelados, mantidos sob refrigeração a 5°C e avaliados durante 24 horas. Além dos parâmetros de cinética espermática foram avaliadas a morfologia e a integridade da membrana plasmática dos espermatozoides. A capacidade de fertilização do sêmen foi avaliada a partir das amostras descongeladas no melhor tempo. Todos os parâmetros de cinética espermática apresentaram valores superiores quando as amostras de sêmen foram descongeladas por 90s em relação ao tempo de 70s, independentemente do tipo de criotubo. Não foram observadas diferenças significativas nos parâmetros de cinética espermática pós-descongelamento entre as amostras congeladas nos criotubos de 1,6 e 4,5 mL, com exceção da MT que foi superior nos criotubos de 1,6 mL (47±14%) em comparação com os criotubos de 4,5 mL (40±11%), independentemente do tempo de descongelamento. Após ativação, os espermatozoides reduziram significativamente os valores dos parâmetros de cinética dentro de 37 segundos, exceto a MT que se manteve constante neste período. Baseando-se na maior parte dos parâmetros espermáticos avaliados (VCL, VSL, VAP e Integridade da membrana plásmatica para ambos os criotubos e MT, MP e Morfologia espermática somente para o criotubo de 1,6 mL) o sêmen congelado manteve sua qualidade durante 3h após o descongelamento. A taxa de fertilização obtida com o sêmen in natura (74±6%) foi superior ao sêmen criopreservado (1,6 mL - 45±9% e 4,5 mL - 41±12%). Os dois criotubos não diferiram entre si neste parâmetro. Uma alta correlação significativa (p<0,05) foi observada entre a fertilização e a cinética espermática (MT - 89%; MP - 86%; VCL - 79%; VSL - 69% e VAP - 78%). Conclui-se que os criotubos de 1,6 e 4,5 mL podem ser utilizados na criopreservação do sêmen de tambaqui, sendo recomendado seu descongelamento a 60°C por 90s e seu uso em procedimentos de fertilização dentro de 3 horas após o descongelamento desde que mantido a 5°C.
Faria, Surian Guerios. "Estabelecimento e caracterização de um banco de células de trabalho para produção da enzima Taq DNA polimerase." Universidade Tecnológica Federal do Paraná, 2017. http://repositorio.utfpr.edu.br/jspui/handle/1/2729.
Повний текст джерелаThe Molecular Biology Institute of Paraná (Instituto de Biologia Molecular do Paraná - IBMP) produces diagnostic kits for the Brazilian Unified National Health System (Sistema Único de Saúde - SUS), that consist in molecular tests, carried out by polymerase chain reaction (PCR). This reaction is performed by the enzyme Taq DNA Polymerase (Taq). The IBMP manufactures Taq from bacterial cell culture in accordance with Good Manufacturing Practices (GMP) and intends to produce it from a cell bank using cells from the same clone in order to increase the homogeneity and reproducibility of the production process. The objective of the present work is to establish a working cell bank (WCB) and to evaluate its stability for the production of the Taq enzyme, starting from a master cell bank (MCB) established in BioManguinhos. The WCB establishment consists of cultivating MCB colonies, characterizing them, evaluating performance, and electing proper cells to compose the WCT. The stability study contemplates tests to prove that cells retain the ability to produce Taq over time (i.e., cell viability, plasmid stability, growth kinetics, expression induction, plasmid DNA extraction and dosage, restriction analysis and plasmid evaluation). The decisive discretion for the selection of one of the clones to compose BCT was the result of growth kinetics. Stability was studied in 10 month-to-month evaluations. In them, cell viability was higher than 1,0 x 106 CFU/mL, showing that the cells remain capable of performing their metabolism and reproduction. Growth to the exponential phase occurred between 6 and 7 hours of culture, with a specific growth rate greater than 0.4 min-1 . The cultures with 1 mM IPTG expressed Taq DNA polymerase enzyme, revealing the qualification of the cells to the intended purpose. The plasmid stability was greater than 90%, indicating that the plasmid pBioMTaq remains stable and has good replication ability. The mean plasmid concentration was 338 ng/μL, and in all months it was greater than 100 ng/μL. In the restriction analysis the plasmids were correctly cleaved and in the plasmid evaluation there was adequate amplification of the 500 bp fragment, demonstrating that the plasmid remains intact and stable, with compatible sequences with its construction. The WCB was tested as substrate for a typical IBMP production process of Taq DNA polymerase, presenting satisfactory in all the stages in which it was evaluated. These results indicate that the established bank remained stable over 10 months and is apt to be used as a prototype for a WCB in compliance with GMP.
Philpott, Megan. "The Genetic Consequences of ex situ Conservation of Exceptional Plant Species." University of Cincinnati / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1535467395352645.
Повний текст джерелаGiovani, Arlete Mazzini Miranda. "Estudo comparativo entre o tecido ósseo criopreservado e o conservado em glicerol a 98%." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/5/5140/tde-20082007-130551/.
Повний текст джерелаThe Tissue Bank of Instituto de Ortopedia e Traumatologia do Hospital das Clínicas da Universidade de São Paulo is developing a line of experimental studies with the intention to present new methods of allografts storage. This study has the objective to compare the method of cryopreservation (-80 ºC) with glycerol 98% preservation at room temperature. It even analyses, in such way, the inhibition capacity of bacterial and fungal growth and any eventual histological changes. For this, 30 samples of trabecular bone tissue have been collected from 10 patients submitted to total hip arthroplasty. These samples were separated in three groups of 10 specimens, as following: a control group, a cryopreserved group and a glycerol 98% preserved group at room temperature. They were stored during a period of one year. The results were analysed by the McNemar statistic method, with a significance of 0,10. Concerning to bone matrix, no significant changes occurred to the two studied groups compared to the control group. Bacterial and fungal growth do not occurred in the stored samples for one year in glycerol 98%. For being extremely reduced when compared with cryopreservation method, the preservation cost in glycerol 98% indicates its use on small sum tissue banks. However, posterior studies about biomechanical properties, glycerol removal of bone tissue and their biological process of integration with the receiver are necessary.
CARVALHO, Maria da Conceição. "Criopreservação de tecido testicular de cães : avaliação histológica e ultraestrutural." Universidade Federal Rural de Pernambuco, 2016. http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4805.
Повний текст джерелаMade available in DSpace on 2016-06-17T12:51:11Z (GMT). No. of bitstreams: 1 Maria da Conceicao Carvalho.pdf: 3109620 bytes, checksum: 2f7f0bd4b88adbfe9f8dee53321968f7 (MD5) Previous issue date: 2016-02-25
Canids are part of the large number of endangered species. The survival of these species depends on the conservation of existing biodiversity. The use of gamete preservation techniques associated with reproductive technologies such as artificial insemination (AI), in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) were developed to help propagate and preserve the genetic potential of canídeos.A testicular tissue cryopreservation might be a method used when ejaculate freezing techniques is not possible. This work set in two experimentos congelamento and vitrificaçãoin vitro, comparing different cryoprotectants (glycerol, dimetisufóxido (DMSO) and trehalose) in testicular tissue of domestic dogs (Canis familiaris). Fragments of 9 adult dogs testes were subjected to a cooling with 4 cryopreservation protocols: slow freezing with glycerol, dimethylsulfoxide (DMSO) or solid surface vitrification using glycerol or DMSO. Fragments of 3mm testicular parenchyma were separated into groups: control, subjected to slow freezing and vitrification. Histological evaluations of the fragments were performed before, after freezing and thawing by light and electron microscopy. Based on morphology and ultrastructure, the testicular tissue of slow freezing, there was no difference between the cryoprotectants. Among the vitrified groups, exposure to DMSO produced a greater structural integrity and architecture when compared to the glycerol group. Comparison of slow freezing and vitrification have shown that vitrification samples revealed more area consisting of tubular compartment, tubular lumen, seminiferous epithelium and conserved membrane. Furthermore, the intertubular compartment Leydig cells showed normal morphology and had characteristics typical of steroidogenic cells. From the results, it was concluded that vitrification DMSO is the most effective method for criopresevar testicular tissue of adult dogs, and can be used as a routine procedure.
Os canídeos fazem parte do grande número de espécies ameaçadas de extinção. A sobrevivência destas espécies depende da conservação da biodiversidade existente. O uso de técnicas de preservação de gametas associadas às tecnologias reprodutivas tais como, inseminação artificial (IA), fertilização in vitro (FIV) e injeção intracitoplasmática de espermatozoides (ICSI) foram desenvolvidas para ajudar a propagar e preservar o potencial genético dos canídeos.A criopreservação de tecido testicular pode vir a ser um método utilizado quando técnicas de congelação do ejaculado não é possível. A presente dissertação configurou-se em dois experimentoscongelamento e vitrificaçãoin vitro, comparando diferentes crioprotetores (glicerol, dimetisufóxido (DMSO) e trealose) em tecido testicular de cães domésticos (Canis familiaris). Fragmentos de testículos de 9 cães adultos foram submetidos a um arrefecimento com 4 protocolos de criopreservação: congelamento lento com glicerol, dimetilsulfóxido (DMSO), ou vitrificação superfície sólida, utilizando glicerol ou DMSO. Os Fragmentos de 3mm do parênquima testicular foram separados em grupos: controle, submetido ao congelamento lento e vitrificação. As avaliações histológicas dos fragmentos foram realizadas antes, após congelação e descongelação por microscopia óptica e eletrônica. Com base na morfologia e ultraestrutura, o tecido testicular de congelação lenta, não houve diferença entre os crioprotetores. Entre os grupos vitrificados, a exposição ao DMSO produziu maior integridade da estrutura e arquitetura quando comparado ao grupo de glicerol. A comparação do congelamento lento e vitrificação demonstraram que a vitrificação revelou amostras com mais área composta por compartimento tubular, luz tubular, epitélio seminífero e membrana conservada. Além disso, o compartimento intertubular mostrou células de Leydig tinham morfologia normal e características típicas de células esteroidogênicas. Diante dos resultados concluiu-se que a vitrificação com DMSO é o método mais eficaz para criopresevar tecido testicular de cães adultos, podendo ser utilizado como procedimento de rotina.
Terraciano, Paula Barros. "Vitrificação versus congelamento lento não automatizado em tecido ovariano de camundongos CF1." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2016. http://hdl.handle.net/10183/151510.
Повний текст джерелаIntroduction: The high prevalence of cancer and the significant increase in long-term survival have generated interest as the preservation of fertility in young women exposed to chemotherapy and radiotherapy. Experimental techniques have been tried in an attempt to reverse the ovarian failure induced by these treatments. In this regard studies of ovarian tissue freezing for subsequent transplantation disclose a new application perspective in the treatment and prevention of female infertility. Objective: two ovarian tissue freezing protocols were tested, a non-automated slow-freezing and by vitrification, in order to assess the viability of the tissues for subsequent autologous transplantation. Methods: as ovaries donors, were used 30 female CF1 mice approximately 8 weeks and weighing 29,29g±2,9. • The ovaries were vitrified or frozen, stored in liquid nitrogen for 30 days and thawed. After thawing, the left ovary was intended for histological and immunohistochemical characterization by histochemical marker for MVH and right ovary was used for the tests with cell viability by trypan blue exclusion. Results: In HE slides was counting primordial, primary, pre antral and antral follicles. No significant difference was found in the proportion of high-quality primordial, primary and pre antral follicles after thawing/warming in the slow-freezing and vitrification group, respectively. The antral follicle counting was significant higher in vitrification group (p=0,004). In immunohistochemistry assay for MVH Antibody , MVH+ and MVH- follicles were counted and compared with the total number of follicles and slow freeze group had a higher number of not marked cells (p=0,012). Conclusion: Although both protocols showed similar results in the histological analysis for follicular counts, the vitrification protocol was significantly better for preserve the ovarian stem cell population.
Feuillassier, Lionel. "La cryoconservation : un outil performant pour la sauvegarde des coraux en danger : son application à Pocillopora damicornis." Thesis, Montpellier, 2015. http://www.theses.fr/2015MONTS177/document.
Повний текст джерелаNumerous environmental and anthropic pressures threaten reef ecosystems, rising concerns on species loss in coming years. Among conservation measures, cryopreservation ensures the safe and cost-effective long-term conservation of biological material. The first publications focusing on Anthozoa cryopreservation reported that the vitrification approach was preferable to the slow-cooling approach. In this context, this thesis aimed at investigating a vitrification technique with several pluricellular forms of the Scleractinian Pocillopora damicornis including apexes, planulae, primary polyps, isolated polyps and tissue balls (TB). The best results were obtained using TBs produced by exposing coral branches to a KSW solution. TBs were cryopreserved using the V Cryo-plate method. The highest TB tolerance was obtained after exposure to solution containing ethylene glycol (EG) concentrated to 4.0 M for 20 min at room temperature (RT). Binary and ternary cryoprotectant (CPA) solutions were better tolerated by TBs compared with individual cryoprotectant solutions. Exposure of TBs to a series of cryoprotectant solutions with progressively increased concentration allowed obtaining TB tolerance to cryoprotectant with a concentration of 4.5 M with: 1.5 M EG + 0.5 M Glycerol (Gly) (5 min, RT), 1.5 M DMSO + 1.5 M EG + 1.5 M Gly (10 min, 0°C) and then 1.5 M EG + 0.5 M Gly (5 min, RT). Epithelial cells from the ectoderm were essential to maintain TB integrity during and following CPA treatments. Successful cryopreservation was not achieved in this work; however, it demonstrated that the use of TBs constitutes a promising way for further cryopreservation research
Commin, Loris. "Cryoconservation du tissu ovarien et production d’embryons chez la chienne." Thesis, Lyon 1, 2012. http://www.theses.fr/2012LYO10116.
Повний текст джерелаNowadays, cryopreservation is widely used in animal assisted reproduction or safeguarding of genetic resources. Nevertheless, the bitch is a complex animal model concerning the use of this biotechnology, due to numerous anatomical and physiological peculiarities. The aim of our research work was to investigate and develop a method of cryopreservation of genetic resources in the bitch by exploring two kinds of resources: embryos and ovarian tissue. After the setting up of a method for embryo collection, we have built up a stock of cryopreserved embryo for subsequent embryo transfer. After a preliminary validation of our in vitro assessment methods, the investigation and development of a cryopreservation protocol has been conducted. The use of fractional experimental design allowed us to highlight the main factors affecting the follicular pool quality (CPA nature, freezing rate and equilibration steps). The combination of DMSO incorporated in a unique equilibration bath with a freezing rate of 0.3°C/min appeared to be suitable for the cryopreservation of bitch ovarian tissue. Finally, Follicular growth and hormonal activity resumption have been observed after xenotransplantation of cryopreserved bitch ovarian tissue
Mutsenko, Vitalii [Verfasser], Birgit [Akademischer Betreuer] Glasmacher, Oleksandr [Akademischer Betreuer] Gryshkov, Thomas [Akademischer Betreuer] Illig, and Stephan [Akademischer Betreuer] Kabelac. "Cryopreservation of mesenchymal stromal cells within tissue engineering approaches / Vitalii Mutsenko ; Akademische Betreuer: Birgit Glasmacher, Oleksandr Gryshkov, Thomas Illig, Stephan Kabelac ; Leibniz Universität Hannover, Institut für Mehrphasenprozesse." Hannover : Bibliothek der Medizinischen Hochschule Hannover, 2019. http://d-nb.info/1197911510/34.
Повний текст джерелаFarooque, Tanya Mahbuba. "Biochemical and mechanical stimuli for improved material properties and preservation of tissue-engineered cartilage." Diss., Atlanta, Ga. : Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/26710.
Повний текст джерелаCommittee Chair: Boyan, Barbara; Committee Chair: Wick, Timothy; Committee Member: Brockbank, Kelvin; Committee Member: Nenes, Athanasios; Committee Member: Sambanis, Athanassios. Part of the SMARTech Electronic Thesis and Dissertation Collection.