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Статті в журналах з теми "Tc cells"
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El Khawanky, Nadia, Amy Hughes, Wenbo Yu, Sanaz Taromi, Jade Clarson, Angel F. Lopez, Michael P. Brown, et al. "Azacytidine Sensitizes AML Cells for Effective Elimination By CD123 CAR T-Cells." Blood 134, Supplement_1 (November 13, 2019): 3904. http://dx.doi.org/10.1182/blood-2019-124684.
Повний текст джерелаАнотація:
Chimeric antigen receptor T-cells (CAR Tc) have yielded impressive remission rates in treatment-refractory B-cell malignancies (B-ALL and B-lymphomas) by targeting CD19, resulting in the first FDA approved CAR Tc therapies, Kymriah and Yescarta. However, the translation of these results for other cancer entities remains a challenge. Pre-clinical studies using second-generation CAR Tc against the interleukin-3 receptor alpha chain (CD123) engendered strong anti-leukemic activity. CD123 CAR Tc clinical studies resulted in transient responses, or complete remission but at the expense of on-target off-tumor toxicities. Our studies employing third-generation anti-CD123 CAR Tc demonstrate strong anti-leukemic activity with no adverse effects in vivo. However, the leukemia was not completely eradicated. Combining anti-CD123 CAR Tc with DNA hypomethylating (HMA) agents may enhance the anti-leukemic effect and survival. HMAs such as azacytidine (Aza) activate key epigenetically silenced pathways in AML cells, inhibiting cell proliferation while enhancing cell immunogenicity. We hypothesized that Aza will increase the expression of CD123 on AML cells resulting in long-term disease eradication by anti-CD123 CAR Tc. The anti-leukemic efficacy, survival advantage, safety and feasibility of the combination treatment with Aza and anti-CD123 CAR Tc were evaluated in vivo. HL-60 (CD123med), MLL-2 (CD123lo), MOLM-13 (CD123hi), primary de novo and relapsed/refractory (r/r) AML cells were cultured for 0-8 days in the presence of Aza (0µM-5µM) and analysed for their CD123 expression by flow cytometry, quantitative western blot and RNAseq. The anti-CD123 CAR was constructed with the humanized CSL362-based ScFv and the CD28-OX40-CD3ζ signaling domain, encoded in a third-generation lentiviral vector and expressed in CD3+ Tc from healthy donors. Rag2γc-/- mice (n=12-16/ group) were engrafted with 1x105 MOLM13/ffLuc AML cells and treated with PBS, 5x106 Non-transduced (NTD) Tc orCAR Tc, 4x 2.5mg/kg Aza, or 5x106 CAR Tc following 4x Aza (2.5mg/kg). Leukemic burden was assessed weekly by bioluminescence imaging. Tc activity and immunophenotyping was performed using flow cytometry at day 35 post engraftment, and survival was monitored. HL-60, MLL-2 and MOLM-13 cells showed significant increases in HLA-DR, PD-L1, STAT1 and IRF7 expression, as well as CD123 when exposed to Aza (Fig 1A,B). Interestingly, the increased effect was seen from day one regardless of concentration. This was similarly reflected in AML patient cells. Aza treatment also arrested cell proliferation and decreased viability in both cell lines and patient cells suggesting Aza can aid in the anti-leukemic effect. Rag2γc-/- mice engrafted with MOLM-13 and treated with Aza and CD123 CAR Tc demonstrated suppressed growth, and eradication of MOLM-13 cells compared to mice treated with CD123 CAR Tc or Aza alone. Additionally, a significant decrease in residual CD123+ cells in the bone marrow (BM) of dual treated mice was seen (Fig 1C). A higher frequency of residual CD8+ T-cells in the BM, and CD4+ Tc in the peripheral blood (PB) and BM of dual treated mice was observed compared to CAR Tc only treated mice. Most prominently, we found a significantly higher mean number of stem cell-like and central memory CD8+ Tc in the BM of dual treated mice (232 cells/µl and 208cells/µl, respectively) compared to the CAR Tc only group (55 cells/µl and 23 cells/µl, respectively). Assessment of immune checkpoint markers on residual CAR Tc of dual treated mice revealed significantly decreased levels of CTLA-4, PD-1 and TIM-3 in the BM, and CTLA-4 in the PB compared to the CAR Tc only group. While CAR Tc treatment alone demonstrated a survival advantage compared to PBS, NTD or Aza treated mice, Aza and CAR Tc treatment had a significantly higher survival rate compared to the CAR Tc only group (92% vs. 46% at day 50, p<.01). Our findings indicate that Aza increases immunogenicity and augments the cell surface expression of CD123 on AML cells, allowing enhanced recognition and elimination of malignant cells by CD123 CAR Tc. This is the first demonstration that HMAs and CAR Tc immunotherapy can be used synergistically to treat AML. Considering HMAs are currently under clinical investigation in AML, our data encourage further clinical evaluation of this dual treatment in r/r AML, including high-risk patients that are chemotherapy or allogeneic transplantation ineligible. Disclosures Hughes: Novartis, Bristol-Myers Squibb, Celgene: Research Funding; Novartis, Bristol-Myers Squibb: Consultancy, Other: Travel. White:BMS: Honoraria, Research Funding; AMGEN: Honoraria, Speakers Bureau. Yong:Novartis: Honoraria, Research Funding; Celgene: Research Funding; BMS: Honoraria, Research Funding.
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Kos, F. J., and E. G. Engleman. "Requirement for natural killer cells in the induction of cytotoxic T cells." Journal of Immunology 155, no. 2 (July 15, 1995): 578–84. http://dx.doi.org/10.4049/jimmunol.155.2.578.
Повний текст джерелаАнотація:
Abstract Cell-mediated immunity involves the participation of both regulatory and cytotoxic cells. The conversion of precursors to effector CD8+ cytotoxic T (Tc) cells requires cell-cell collaboration in which CD4+ T cells are traditionally viewed as helper cells. An in vitro system was used here to demonstrate that the generation of human alloantigen-specific CD8+ Tc cells requires the participation of CD3-CD16+CD56+ NK cells but not CD4+ T helper cells. Depletion of NK cells from responders abolished the induction of alloantigen-specific Tc cells in mixed lymphocyte cultures (MLC). Purified CD5+CD8+ T cells stimulated with alloantigen proliferated but did not differentiate into fully functional effector Tc cells. Coculture of responder CD5+CD8+ T cells with NK cells promoted the conversion of CD8+ Tc cell precursors (pTc) into effector Tc cells. Anti-CD56 mAbs blocked Tc cell induction in MLC, suggesting a role for CD56 molecules expressed on NK cells in either alloantigen recognition or delivery of accessory signals to pTc cells. These findings suggest a novel critical link between the natural and specific immune responses.
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Kim, Sung Man, Eun Ju Lee, Hye Sook Jung, Na Han, You Jeong Kim, Tae Kyoon Kim, Tae Nyun Kim та ін. "Co-Culture of α TC-6 Cells and β TC-1 Cells: Morphology and Function". Endocrinology and Metabolism 30, № 1 (2015): 92. http://dx.doi.org/10.3803/enm.2015.30.1.92.
Повний текст джерела
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Inoue, Tsuyoshi, and Keiji Imoto. "Feedforward Inhibitory Connections From Multiple Thalamic Cells to Multiple Regular-Spiking Cells in Layer 4 of the Somatosensory Cortex." Journal of Neurophysiology 96, no. 4 (October 2006): 1746–54. http://dx.doi.org/10.1152/jn.00301.2006.
Повний текст джерелаАнотація:
Thalamocortical (TC) cells in the ventrobasal thalamus make direct excitatory connections with regular-spiking (RS) cells in layer 4 of the somatosensory cortex, but also make disynaptic feedforward inhibitory connections with the RS cells by layer 4 fast-spiking (FS) cells. In this study, we investigated connection rules of the feedforward inhibitory circuit from multiple TC cells to multiple RS cells, at the level of synaptic potentials. Using thalamocortical brain slices of young mice (postnatal days 12–16), we made simultaneous patch-clamp recordings from three adjacent cortical cells (two RS cells and one FS cell), combined with minimal stimulation of presumed single TC fibers. We found that nearly all (97%) of TC fibers, which generated excitatory inputs onto RS cells, also generated divergent excitatory inputs onto adjacent FS cells. Some 44% of TC fibers generated divergent excitatory inputs onto adjacent pairs of RS cells. We then combined the triple patch-clamp recording with multisite (two to three) minimal stimulation of single TC fibers and found that 86% of FS cells received convergent inputs from all of the stimulated TC fibers. We also found that 68% of FS cells generated divergent inhibitory inputs onto adjacent pairs of RS cells. The results indicate that spikes in TC cells, which excite RS cells, also excite adjacent FS cells with high fidelity. The results also indicate that FS cells receive convergent excitatory inputs from multiple TC cells and then send divergent inhibitory outputs to multiple RS cells.
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Reuter, Audrey, Chloé Virolle, Kelly Goldlust, Annick Berne-Dedieu, Sophie Nolivos, and Christian Lesterlin. "Direct visualisation of drug-efflux in live Escherichia coli cells." FEMS Microbiology Reviews 44, no. 6 (August 6, 2020): 782–92. http://dx.doi.org/10.1093/femsre/fuaa031.
Повний текст джерелаАнотація:
ABSTRACT Drug-efflux by pump proteins is one of the major mechanisms of antibiotic resistance in bacteria. Here, we use quantitative fluorescence microscopy to investigate the real-time dynamics of drug accumulation and efflux in live E. coli cells. We visualize simultaneously the intrinsically fluorescent protein-synthesis inhibitor tetracycline (Tc) and the fluorescently labelled Tc-specific efflux pump, TetA. We show that Tc penetrates the cells within minutes and accumulates to stable intracellular concentration after ∼20 min. The final level of drug accumulation reflects the balance between Tc-uptake by the cells and Tc-efflux by pump proteins. In wild-type Tc-sensitive cells, drug accumulation is significantly limited by the activity of the multidrug efflux pump, AcrAB-TolC. Tc-resistance wild-type cells carrying a plasmid-borne Tn10 transposon contain variable amounts of TetA protein, produced under steady-state repression by the TetR repressor. TetA content heterogeneity determines the cells’ initial ability to efflux Tc. Yet, efflux remains partial until the synthesis of additional TetA pumps allows for Tc-efflux activity to surpass Tc-uptake. Cells overproducing TetA no longer accumulate Tc and become resistant to high concentrations of the drug. This work uncovers the dynamic balance between drug entry, protein-synthesis inhibition, efflux-pump production, drug-efflux activity and drug-resistance levels.
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Carmel, R., SM Neely, and RB Jr Francis. "Human umbilical vein endothelial cells secrete transcobalamin II." Blood 75, no. 1 (January 1, 1990): 251–54. http://dx.doi.org/10.1182/blood.v75.1.251.251.
Повний текст джерелаАнотація:
Abstract Transcobalamin II (TC II) is essential for cellular uptake of cobalamin. However, the origin of this transport protein is controversial and many organ sources have been suggested. We studied human umbilical vein endothelial cells cultured in vitro. The cells contained TC II (2.3 pmol/10(8) cells) and released progressively increasing amounts of the protein into the surrounding medium during the 3-day incubation period. This release exceeded the starting intracellular content of TC II. In contrast, endothelial cells did not contain or elaborate R binder, the other major circulating binding protein for cobalamin, Cycloheximide inhibited the elaboration of TC II, suggesting that the endothelial cells synthesize the protein. Thrombin, which stimulates tissue plasminogen activator release, did not enhance TC II release, and neither did endotoxin or mellitin. However, thrombin did appear to partially protect TC II release from inhibition by cycloheximide. Among other cells studied, human fibroblasts also released TC II into the incubation medium, while K562 human leukemia cells, ARH-77 and HS Sultan human plasma cell lines, and Raji strain lymphoblasts did not. The data suggest that endothelial cells are an important source of the metabolically crucial TC II.
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Carmel, R., SM Neely, and RB Jr Francis. "Human umbilical vein endothelial cells secrete transcobalamin II." Blood 75, no. 1 (January 1, 1990): 251–54. http://dx.doi.org/10.1182/blood.v75.1.251.bloodjournal751251.
Повний текст джерелаАнотація:
Transcobalamin II (TC II) is essential for cellular uptake of cobalamin. However, the origin of this transport protein is controversial and many organ sources have been suggested. We studied human umbilical vein endothelial cells cultured in vitro. The cells contained TC II (2.3 pmol/10(8) cells) and released progressively increasing amounts of the protein into the surrounding medium during the 3-day incubation period. This release exceeded the starting intracellular content of TC II. In contrast, endothelial cells did not contain or elaborate R binder, the other major circulating binding protein for cobalamin, Cycloheximide inhibited the elaboration of TC II, suggesting that the endothelial cells synthesize the protein. Thrombin, which stimulates tissue plasminogen activator release, did not enhance TC II release, and neither did endotoxin or mellitin. However, thrombin did appear to partially protect TC II release from inhibition by cycloheximide. Among other cells studied, human fibroblasts also released TC II into the incubation medium, while K562 human leukemia cells, ARH-77 and HS Sultan human plasma cell lines, and Raji strain lymphoblasts did not. The data suggest that endothelial cells are an important source of the metabolically crucial TC II.
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Visciano, Carla, Nella Prevete, Federica Liotti, and Gianni Marone. "Tumor-Associated Mast Cells in Thyroid Cancer." International Journal of Endocrinology 2015 (2015): 1–8. http://dx.doi.org/10.1155/2015/705169.
Повний текст джерелаАнотація:
There is compelling evidence that the tumor microenvironment plays a major role in mediating aggressive features of cancer cells, including invasive capacity and resistance to conventional and novel therapies. Among the different cell populations that infiltrate cancer stroma, mast cells (MCs) can influence several aspects of tumor biology, including tumor development and progression, angiogenesis, lymphangiogenesis, and tissue remodelling. Thyroid cancer (TC), the most frequent neoplasia of the endocrine system, is characterized by a MC infiltrate, whose density correlates with extrathyroidal extension and invasiveness. Recent evidence suggests the occurrence of epithelial-to-mesenchymal transition (EMT) and stemness in human TC. The precise role of immune cells and their mediators responsible for these features in TC remains unknown. Here, we review the relevance of MC-derived mediators (e.g., the chemokines CXCL1/GRO-α, CXCL10/IP-10, and CXCL8/IL-8) in the context of TC. CXCL1/GRO-αand CXCL10/IP-10 appear to be involved in the stimulation of cell proliferation, while CXCL8/IL-8 participates in the acquisition of TC malignant traits through its ability to induce/enhance the EMT and stem-like features of TC cells. The inhibition of chemokine signaling may offer novel therapeutic approaches for the treatment of refractory forms of TC.
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Huang, Nick, Brandon Wyman, Emma Cravo, Thomas Winans, Gourav Choudhary, Zachary Oaks, Manuel Duarte, et al. "Rab4A inactivation in T cells blocks mTOR activation, pro-inflammatory lineage development, and disease pathogenesis in lupus-prone mice." Journal of Immunology 202, no. 1_Supplement (May 1, 2019): 115.6. http://dx.doi.org/10.4049/jimmunol.202.supp.115.6.
Повний текст джерелаАнотація:
Abstract Introduction Expression of Rab4A is increased in T cells of patients and mice with SLE. Overexpression of Rab4A forms a positive feedback loop with mTOR that can be blocked with therapeutic efficacy in SLE. To determine the impact of this gene on disease development, the triple-congenic lupus-prone Sle1.2.3 mouse strain (TC) have been backcrossed with C57Bl/6 wild-type (WT) mice that carry floxed Rab4AQ72L (TC-FL) alleles or lack Rab4A in T cells (TC-KO). Methods Proteinuria was assessed by the Bradford assay. Splenocytes were examined by flow cytometry. Autoantibody production was measured by ELISA. Results TC mice had increased proteinuria over age and sex-matched WT controls. Deletion of Rab4A in T cells reduced proteinuria ≥ 50% in female TC-KO mice relative to TC-FL mice at 21 or 40 weeks of age. Similar trends were noted in male mice. The production of antinuclear and antiphospholipid antibodies was reduced in TC-KO mice as compared to TC-FL and parental TC controls. Immunophenotyping unveiled a 45% depletion of CD4+ T cells and 50% expansion of CD8+ T cells in female TC-KO mice relative to TC-FL controls. CD38 expression was reduced on CD4+ T cells of TC-KO mice by 51% and 48% relative to TC and TC-FL controls. CD38 expression was also reduced on CD8+ T cells but not on CD19+ B cells. mTORC1 activity was reduced by 36% in CD4 T cells, but not in CD8 T cells or B cells of TC-KO mice. Along these lines, overexpression of Rab4A activated mTORC1, reduced expression of CD4, and increased expression of CD38 in Jurkat cells. Conclusion These findings reveal an opposite influence of Rab4A on endosomal recycling of CD4 and CD38 that facilitates mTORC1 activation and thus causes pro-inflammatory T-cell lineage specification and triggers autoimmunity in SLE.
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Destexhe, A., T. Bal, D. A. McCormick, and T. J. Sejnowski. "Ionic mechanisms underlying synchronized oscillations and propagating waves in a model of ferret thalamic slices." Journal of Neurophysiology 76, no. 3 (September 1, 1996): 2049–70. http://dx.doi.org/10.1152/jn.1996.76.3.2049.
Повний текст джерелаАнотація:
1. A network model of thalamocortical (TC) and thalamic reticular (RE) neurons was developed based on electrophysiological measurements in ferret thalamic slices. Single-compartment TC and RE cells included voltage- and calcium-sensitive currents described by Hodgkin-Huxley type of kinetics. Synaptic currents were modeled by kinetic models of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), gamma-aminobutyric acid-A (GABAA) and GABAB receptors. 2. The model reproduced successfully the characteristics of spindle and slow bicuculline-induced oscillations observed in vitro. The characteristics of these two types of oscillations depended on both the intrinsic properties of TC and RE cells and their pattern of interconnectivity. 3. The oscillations were organized by the reciprocal recruitment between TC and RE cells, due to their manual connectivity and bursting properties. TC cells elicited AMPA-mediated excitatory postsynaptic potentials (EPSPs) in RE cells, whereas RE cells elicited a mixture of GABAA and GABAB inhibitory postsynaptic potentials (IPSPs) in TC cells. Because of the presence of a T current, sufficiently strong EPSPs could elicit a burst in RE cells, and TC cells could generate a rebound burst following GABAergic IPSPs. Under these conditions, interaction between the TC and RE cells produced sustained oscillations. 4. In the absence of spontaneous oscillation in any cell, the TC-RE network remained quiescent. Spindle oscillations with a frequency of 9-11 Hz could be initiated by stimulation of either TC or RE neurons. A few spontaneously oscillating TC neurons recruited the entire network model into a "waxing-and waning" oscillation. These "initiator" cells could be an extremely small proportion of TC cells. 5. In intracellular recordings, TC cells display a reduced ability for burst firing after a sequence of bursts. The "waning" phase of spindles was reproduced in the network model by assuming an activity-dependent upregulation of Ih operating via a calcium-binding protein in TC cells, as shown previously in a two-cell model. 6. Following the global suppression of GABAA inhibition, the disinhibited RE cells produced prolonged burst discharges that elicited strong GABAB-mediated currents in TC cells. The enhancement of slow IPSPs in TC cells was also due to cooperativity in the activation of GABAB-mediated current. These slow IPSPs recruited TC and RE cells into slower waxing-and-waning oscillations (3-4 HZ) that were even more highly synchronized. 7. Local axonal arborization of the TC to RE and RE to TC projections allowed oscillations to propagate through the network. An oscillation starting at a single focus induced a propagating wavefront as more cells were recruited progressively. The waning of the oscillation also propagated due to upregulation of Ih in TC cells, leading to waves of spindle activity as observed in experiments. 8. The spatiotemporal properties of propagating waves in the model were highly dependent on the intrinsic properties of TC cells. The spatial pattern of spiking activity was markedly different for spindles compared with bicuculline-induced oscillations and depended on the rebound burst behavior of TC cells. The upregulation of Ih produced a refractory period so that colliding spindle waves merged into a single oscillation and extinguished. Finally, reducing the Ih conductance led to sustained oscillations. 9. Two key properties of cells in the thalamic network may account for the initiation, propagation, and termination of spindle oscillations, the activity-dependent upregulation of Ih in TC cells, and the localized axonal projections between TC and RE cells. In addition, the model predicts that a nonlinear stimulus dependency of GABAB responses accounts for the genesis of prolonged synchronized discharges following block of GABAA receptors.
Дисертації з теми "Tc cells"
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BRAMBILLA, TANIA de P. "Desenvolvimento de metodos para marcacao de DMSA pentavalente com sup(99m)Tc e sup(188)Re." reponame:Repositório Institucional do IPEN, 2009. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11516.
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Dissertacao (Mestrado)
IPEN/D
Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP
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Lackay, Carolynn Louise. "Evaluation of the labelling and binding efficiency of Tc-99m to red blood cells of patients who are being exposed to a cocktail of anti-tuberculosis drugs." Thesis, Cape Peninsula University of Technology, 2010. http://hdl.handle.net/20.500.11838/1555.
Повний текст джерелаАнотація:
Thesis (MTech (Radiography (Nuclear Medicine)))--Cape Peninsula University of Technology, 2010Radioactively labelled red blood cells (RBC) are used in various nuclear medicine studies. In order to obtain accurate results when performing these studies, it is of paramount importance that a good binding of the radioactivity (Tc-99m) with the red blood cells is ensured. The literature indicates that certain drugs can influence red cell membrane properties and biochemistry. These drugs can potentially influence the binding of radionuclides to cells. Antibiotics may possibly alter the labelling efficiency of Tc-99m RBC. Due to the high incidence of tuberculosis (TB) in South Africa, many patients receive anti-TB medication, and therefore the influence of these drugs on the labelling efficiencies of Tc-99m RBC was studied.
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TEODORO, RODRIGO. "Avaliação pré-clínica do análogo da neurotensina(8-13) radiomarcado com sup(99m)Tc: caracterização in vitro e in vivo." reponame:Repositório Institucional do IPEN, 2010. http://repositorio.ipen.br:8080/xmlui/handle/123456789/9538.
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Tese (Doutoramento)
IPEN/T
Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP
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Modebe, Emmanuel Obinna. "Extended Cr-51 RBC combined with Tc-99m RBC for the detection and localisation of occult GIT bleeding." Thesis, Stellenbosch : Stellenbosch University, 2014. http://hdl.handle.net/10019.1/86509.
Повний текст джерелаАнотація:
Thesis (MMed)--Stellenbosch University, 2014.ENGLISH ABSTRACT: Background Occult blood loss from the gastrointestinal tract (GIT), causing iron deficiency often with anaemia, can be diagnostically and therapeutically challenging. This is because the endoscopic and radiologic tests may be negative due to the slow, chronic and intermittent nature of the gastrointestinal bleeding, making timing key in detection and localisation of the bleed. These limitations can be approached using two different radioactive isotopes. Firstly, we tested the sensitivity of extending Cr-51 RBC for 21 days relative to 5 days to detect GIT bleeding and its use to optimise timing of a Tc-99m RBC study for GIT blood loss localisation. Finally, we tested if the information provided by the Tc-99m RBC study aided gastroenterologic intervention for anatomical localisation of a lesion. Method In this retrospective review, after obtaining institutional and ethics committee approval, records of patients referred for evaluation of possible GIT blood loss were reviewed. In each; daily appearance of radiochromium in stool was measured in the whole body counter. In those cases exceeding 50 ml/day, a technetium-99m (Tc-99m) localization study was performed. These studies were correlated with clinical findings. Results A total of 59 Cr-51 RBC studies were carried out in 36 females and 21 males (n = 57). In 32 (54%) the radiochromium results were positive with 75% of the bleeding incidences occurring after 5 days of stool collection. Of 17 cases in whom Tc-99m RBC imaging studies were performed, 14 (82%) were positive with specific anatomical sites successfully defined in twelve. In all patients with blood loss of >100 ml/24h, Tc-99m RBC were positive and localised. Ten of the 17 Tc-99m RBC studies were further investigated and half diagnosed with small-bowel angiodysplasia. Conclusion This sequential twin isotope method is practical in revealing otherwise silent intestinal haemorrhage. Although it has good patient acceptability and clinical as well as diagnostic utility in management, further studies are required to clearly establish a cut-off level of blood loss for performing imaging studies and the impact of the findings on the overall patient management.
AFRIKAANSE OPSOMMING: Agtergrond Die evaluasie van okkulte bloedverlies uit die gastro-intestinale kanaal (GIT), met gevolglike ystertekort anemie, kan diagnosties en terapeuties uitdagend wees. Dit is omdat endoskopiese en radiologiese ondersoeke negatief mag wees as gevolg van die stadige, chroniese en intermitterende aard van die gastro-intestinale bloeding, wat die presiese tydstip van opsporing en lokalisering van die bloeding krities belangrik maak. Hierdie beperkings kan aangespreek word deur twee verskillende radioaktiewe isotope te gebruik. Eerstens is die sensitiwiteit van die verlenging van die Cr-51 RBS studie tot 21 dae in plaas van 5 dae om die GIT bloeding op te spoor, getoets, asook die gebruik daarvan om die optimale tyd vir ‘n Tc-99m RBS studie om die GIT bloedverlies te lokaliseer, vas te stel. Laastens is getoets of die inligting van die Tc-99m RBS studie wel bygedra het tot die gastroenterologiese ingreep om die letsel anatomies te lokaliseer. Metode Na institusionele en etiese komitee toestemming is inligting van pasiënte wat vir die evaluering van ‘n moontlike GI bloedverlies verwys is, in hierdie retrospektiewe oorsig nagegaan. Die daaglikse voorkoms van radioaktiewe chroom in stoelgangmonsters is in ‘n heelliggaamteller gemeet. In gevalle waar dit 50 ml/dag oorskry het, is ‘n tegnesium 99m (Tc 99m) studie gedoen. Hierdie studies is met die kliniese bevindinge gekorreleer. Resultate ‘n Totaal van 59 Cr-51 RBS studies is in 36 vroue en 21 mans (n = 57) gedoen. Die gemerkte chroomstudies was positief in 32 (54%), met 75% van die bloedings wat meer as 5 dae na versameling van die stoelgang plaasgevind het. In veertien (82%) van die 17 gevalle waar Tc-99m RBS studies gedoen is, was die studies positief. Spesifieke anatomiese gebiede van bloeding kon in 12 hiervan suksesvol bevestig word. Tc-99m RBS studies was positief in al die pasiënte met ‘n bloedverlies van >100 ml/24h, en kon gelokaliseer word. Tien van die 17 Tc-99m RBS studies is verder ondersoek en die helfte daarvan gediagnoseer met dunderm angiodisplasie. Gevolgtrekking Die opeenvolgende twee isotoopmetode om andersins asimptomatiese dermbloeding op te spoor, is prakties uitvoerbaar. Alhoewel die studies goed deur pasiënte aanvaar is, en ook van kliniese en diagnostiese waarde in die hantering van die pasiënte is, is verdere studies nodig om die afsnypunt vir die hoeveelheid bloedverlies om beeldingstudies uit te voer, sonder twyfel vas te stel, asook om die impak van die bevindings op ‘n groter pasiëntpopulasie vas te stel.
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Rodrigues, Michelle Pinheiro. "Avaliação da toxicidade induzida pelos componentes do radiofármaco 99m Tc-MDP em cepa de E. coli AB1157 e células eucarióticas de ratos e humanos." Universidade do Estado do Rio de Janeiro, 2008. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=1601.
Повний текст джерелаАнотація:
Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorAs células dos seres vivos são constantemente ameaçadas por agentes químicos ou físicos que possam causar danos ao DNA. Um dos agentes deste estudo foi o cloreto estanoso (SnCl2), utilizado na medicina nuclear como redutor de um isótopo radioativo do tecnécio, o 99mTc. O SnCl2 é um agente cujo mecanismo de produção de lesões em estruturas celulares envolve a geração de espécies reativas de oxigênio (ERO), tais como o H2O2 e o radical OH. Essas ERO podem causar uma série de doenças, o envelhecimento e até mesmo a morte celular, por apoptose ou necrose. Sendo assim, torna-se importante a pesquisa sobre os efeitos biológicos, tanto desse sal, quanto das outras substâncias que compõem os radiofármacos utilizados em medicina nuclear. Desta forma, nosso objetivo geral foi avaliar a toxicidade do cloreto estanoso, associado, ou não, ao kit 99mTc-MDP, bem como dos demais componentes do kit, em diferentes sistemas biológicos. Eletroforese em gel alcalino de agarose em células de E. coli AB 1157 para a avaliação da genotoxicidade; Ensaio do Cometa em células de sangue total de ratos Wistar para estudar a genotoxicidade; Ensaio do Micronúcleo em células da medula óssea de ratos Wistar para verificar o potencial aneugênico e clastogênico; Ensaio Cometa em células de sangue total e em células mononucleares de sangue periférico humano para estudar a genotoxicidade; Ensaio de Viabilidade com Azul Trypan e citometria de fluxo para analisar a citotoxicidade em PBMC; Ensaio do Micronúcleo em linfócitos humanos para verificar o potencial aneugênico e clastogênico. Em cepas de E. coli AB1157, o SnCl2 e o MDP induziram quebras no DNA genômico, quando isolados; porém quando usados de forma associada, ocorreu uma atenuação do número de quebras. Em ratos Wistar, o 99mTc-MDP não foi genotóxico e também não induziu clastogênese ou aneugênese. Em Sangue total, in vitro, o SnCl2 apresentou efeito dose-resposta. Em PBMC, in vitro, o 99mTc-MDP causou redução da viabilidade celular e apresentou genotoxicidade, porém não induziu clastogênese e nem aneugênese.
Alive cells are constantly threated by chemical and physical agents that can generate DNA damage. Here, the studied agent was stannous chloride (SnCl2), a 99mTc reducing agent employed in nuclear medicine. This salt can produce lesions through generation of reactive oxygen species (ROS) as H2O2 and OH. These ROS can be the origin of several diseases and cell death by apoptosis or necrosis. In this way it is important the research about the biological effects of this salt and the other substances composing the radiopharmaceuticals used in nuclear medicine.The aim of this work was to evaluate, in different biological systems, the stannous chloride toxic potentiality, associated or not to 99mTc-MDP radiopharmaceutical as well as the other kit components. Alkaline electrophoresis agarosis gel of E. coli AB 1157 to evaluate the genotoxicity in prokariotic cells; Comet assay in Wistar rats total blood to evaluate the genotoxicity in eukaryotic cells; Micronucleous assay in Wistar rats bone marrow cells to verify the aneugenic and clastogenic effects; Comet assay in human in peripherical total blood and mononuclear cells to evaluate the genotoxicity; Trypan blue viability assay and flow cytometry to evaluate citotoxicity in PBMC; Micronucleous assay in human lymphocyte cells to verify the aneugenic and clastogenic effects; SnCl2 and MDP when isolated induced breaks in E. coli genomic DNA. But, when used in an associated way it was observed an atenuation of the breaks number. 99mTc-MDP was not genotoxic, clastogenic nor aneugenic in Wistar rats In ex vivo human total blood, SnCl2 presented a dose-response effect In ex vivo PBMC; 99mTc-MDP induced a cellular viability reduction and presented genotoxic but not clastogenic or aneugenic effects.
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Brunet, Daniel V. "The role of T-cell protein tyrosine phosphatase (TC-PTP) in the nucleus /." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=97913.
Повний текст джерелаАнотація:
The 45kDa isoform of TC-PTP is a ubiquitous, predominantly nuclear protein. Reports demonstrate that it can exist outside the nucleus; however, immunofluorescence and cellular fractionation techniques point to an almost exclusive residence in the nucleus, and even within the nucleolus. Though the conditions of nucleolar enrichment appear to be serum-dependent, and may involve S52 phosphorylation, the exact circumstances necessary for this localization remain unknown, though TC-PTP C216S mutant, and not an S52A/C216S double mutant had the ability to trap a phosphorylated substrate of ∼105kDa, thus encouraging further research into the identity of this phosphoprotein. There is also data implicating TC-PTP in the p53-dependent DNA damage response. TC-PTP WT pMEF's are more susceptible to apoptosis than KO pMEF's, thus suggesting a possible role as a tumor suppressor. Finally, in order to explore the physiological significance of possible non-hematopoietic roles of TC-PTP, a Cre-Lox targeting vector was constructed for the eventual generation of a conditional knockout.
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Karlsson, Susann. "T-Cell Protein Tyrosine Phosphatase, a Regulator of the PDGF Signaling Pathway." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-107674.
Повний текст джерела
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Santos, Denise Takehana dos. "Mapeamento topográfico metabólico de carcinomas espinocelulares de cabeça e pescoço utilizando a fusão de imagens 18 F-FDG PET - TC." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/23/23139/tde-09082005-124541/.
Повний текст джерелаАнотація:
O objetivo desta pesquisa foi estabelecer uma metodologia para avaliar carcinomas espinocelulares (CEC) de cabeça e pescoço, identificando e distinguindo áreas de maior atividade metabólica dentro da neoplasia, associando dados simultaneamente adquiridos, obtidos por diferentes modalidades de aquisição de imagens, combinando informações metabólicas e anatômicas num único exame. A população estudada consistiu de 17 pacientes com carcinoma espinocelular (CEC) de cabeça e pescoço pertencentes aos arquivos do Departamento de Imagem do Hospital do Câncer, São Paulo. As imagens de TC (tomografia computadorizada) e do 18 F-FDG-PET (tomografia por emissão de pósitrons) foram simultaneamente adquiridas utilizando um aparelho não dedicado. Os dados originais foram transferidos para uma estação de trabalho independente com programa de computação gráfica para o processamento dos grupos individuais e fusão em um único grupo, contendo os dados fisiológicos e metabólicos. Os achados foram definidos como positivos na presença de focos com aumento da concentração do radiofármaco em áreas não relacionadas à distribuição normal do mesmo. Em 77% dos casos (n=13), a hipercaptação foi detectada ao centro da lesão e em 23% (n=4) dos casos houve comportamento diferente, com hipercaptação excêntrica. A fusão de imagens simultaneamente adquiridas num único exame ( 18 F- FDG PET e TC) possibilitou o mapeamento topográfico metabólico das lesões estudadas e foi possível localizar áreas de maior atividade metabólica dentro do próprio 13 tumor, verificando recidivas ou metástases, possibilitando aumentar as opções quanto ao planejamento radioterápico ou cirúrgico a serem seguidos.The aim of this study is to propose a methodological approach to evaluate head and neck squamous cell carcinoma (SCCA) in order to identify and to distinguish areas of higher metabolic activity inside the lesion combining the functional metabolic and morphological data simultaneously acquired in a non dedicated PET-CT device. The study population consisted of 17 patients, with SCCA of the head and neck carcinoma. These patients were submitted to a non-dedicated 18 F- FDG- PET imaging using a system with low dose CT and Positron emission coincidence acquisition capabilities. The image acquisition was then transferred to an ENTEGRA 2 NT workstation to generate groups of individual images (metabolic and anatomical data) and image fusion (CT + PET). In those patients with anomalous concentrations of 18 F-FDG, the lesion was depicted on three planes (axial, coronal and sagittal) in CT, PET, and the image fusion at the computer screen. The findings were defined as positive in the presence of well-defined focal area of increased uptake in regions unrelated to the normal biodistribution of the tracer on visual inspection. Two examiners interpreted the images in different sessions, in order to get an agreement. Subsequently, the sites of higher metabolic activity inside the tumor were identified and classified in centric or eccentric, according to their relative location. Observing the images, we found 77.00% of the patients with the site of higher activity at the center of lesion. In 23.00% of the patients a different 15 behavior, with the tracer increased eccentrically to the lesion. This technique gave a realistic view of the functional metabolism, locating the anatomical tumor area and helping in future treatment planning.
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Mestre, Torres Jaume. "Tesi doctoral: factors pronòstics en la pèrdua visual en l’arteritis de cèl·lules gegants." Doctoral thesis, Universitat Autònoma de Barcelona, 2019. http://hdl.handle.net/10803/669749.
Повний текст джерелаАнотація:
L’arteritis de cèl·lules gegants és la vasculitis més freqüent en persones majors de 50 anys. Es classifica segons els criteris de Hunder (1990), essent la cefalea el símptoma més freqüent. L’afectació vascular s’ha estudiat de forma extensa amb diferents proves d’imatge i amb la biòpsia de l’artèria temporal, havent-se suggerit que existeixen dos subtipus de la malaltia. Es coneix que una proporció elevada de pacients presenta aortitis i que aquesta s’associa a una major dosi acumulada de corticoides i a un major risc d’aneurisma d’aorta toràcica. Per altra banda, l’afectació ocular constitueix la complicació més temuda en l’actualitat, ja que un cop instaurada no és reversible.
L’objectiu d’aquest treball va ser identificar quins són els patrons de captació per PET/TC en el moment del diagnòstic de l’ACG d’acord amb la presència o absència de clínica isquèmica, incloent l’afectació ocular. A més, s’estudià quins eren els patrons clínics i histològics en aquesta cohort de pacients d’acord a la presència o absència d’aortitis en el PET/TC.
En el primer estudi, realitzat amb una cohort de pacients amb ACG de nou diagnòstic, s’inclogueren 30 pacients i s’objectivà que els pacients amb clínica isquèmica presentaven una major proporció d’afectació d’artèries vertebrals (OR 5.0, IC 95%: 0.99 – 24.86, p=0.051). Aquests pacients, en canvi, presentaven una menor proporció d’afectació d’artèries de gran cabal, essent l’aortitis un factor protector tant per la clínica isquèmica, en global (OR 19.0, IC 95%: 2.79 – 127.97, p=0.001), com per la pèrdua de visió permanent (OR 10.67, IC 95%: 1.12 – 101.34, p=0.04). En aquesta cohort, la presència de símptomes isquèmics s’associà a patrons de PET/TC diferenciats (p=0.001).
En el segon estudi es demostrà que els pacients amb aortitis eren més joves (69.9 anys vs. 83.7 anys, p=0.04) i presentaven menys freqüentment clínica isquèmica, incloent clínica ocular (25.0% en els pacients amb aortitis vs. 84.2% en els pacients sense aortitis, p=0.006). La presència de cèl·lules gegants multinucleades en la biòpsia de l’artèria temporal s’associà de forma independent a la presència d’aortitis en el PET/TC (OR 12.23, p=0.046). En el grup de pacients amb aortitis no s’objectivà halo en el Doppler d’artèria temporal en cap cas.
Així, podem concloure que el PET/TC és una eina útil en el diagnòstic de l’ACG, que permet identificar dos patrons de captació vascular diferents d’acord a la presència o absència de clínica isquèmica al diagnòstic de l’ACG. Així mateix, la presència d’aortitis en el PET/TC s’associa amb la presencia de cèl·lules gegants multinucleades en la biòpsia.Giant cell arteritis is the most frequently diagnose vasculitis among patients older than 50 years. Classification is done by Hunder criteria (1990). Headache is the most frequent symptom. Vascular involvement has been extensively studied by imaging techniques, including temporal artery biopsy, suggesting the existence of two different clinical subsets of the disease. Evidence suggests that a high proportion of patients present with aortitis and that this situation is associated to a higher corticosteroid dose requirement and a higher risk of developing aortic aneurisms. Moreover, ocular involvement is the most feared complication nowadays as it is usually irreversible. The objective of this study was to identify different patterns of vascular involvement on PET/CT at GCA diagnosis according to the presence or absence of ischaemic manifestations at disease onset, including ocular involvement. Furthermore, we sought to describe clinical and histological patterns on TAB according to the presence or absence of aortitis in PET/CT. In the first study, performed with a cohort that comprised 30 patients with a newly diagnosed GCA, patients with ischaemic symptoms showed a higher proportion of vertebral involvement when compared to those patients without these symptoms (OR 5.0, CI 95%: 0.99 – 24.86, p=0.051). Patients with ischaemic manifestations showed a lower proportion of large vessel vasculitis, being aortitis a protector factor against ischaemic manifestations (OR 19.0, CI 95%: 2.79 – 127.97, p=0.001) and against permanent visual loss (OR 10.67, CI 95%: 1.12 – 101.34, p=0.04). In this cohort, the presence or absence of ischaemic symptoms was associated to different vascular patterns of PET/TC (p=0.001). In the second study we showed that patients with aortitis were younger (69.9 years vs. 83.7 years, p=0.04) and had less frequently ischaemic manifestations, including ocular involvement (25.0% of patients with aortitis vs. 84.2% of patients without aortitis, p=0.006). The presence of giant multinucleated cells on TAB was an independent risk factor for the presence of aortitis on PET/CT (OR 12.23, p=0.046). The halo sign was absent in patients with aortitis. We can conclude that PET/CT is a useful technique at GCA diagnosis that allows identifying two patterns of vascular involvement according to the presence or absence of ischaemic symptoms. When we compared PET/CT and TAB findings, we identified that aortitis was related to the presence of giant multinucleated cells.
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Kirlangic, Ozgur Ulas. "Generation Of Surface Waves Due To Sudden Movements At The Sea Bottom." Master's thesis, METU, 2004. http://etd.lib.metu.edu.tr/upload/12604968/index.pdf.
Повний текст джерелаАнотація:
A mathematical model is developed for investigating time dependent surface deformations of a hydrostatic water volume, when it is subjected to a sudden partial collapse or rise of the sea bottom.
The model solves two-dimensional Navier-Stokes Equations on a vertical plane numerically by using Marker and Cell Method (MAC) for viscous and compressible fluid including all the nonlinear effects in the solution.
For demonstration, a vertical motion was given to a section in a hypothetical reservoir bed within a short time period and the resulting velocity and pressure fields and the surface profile of the water body are obtained. Computational and physical aspects are discussed.
Книги з теми "Tc cells"
1
Solá, Susana, and Joana Paiva Miranda, eds. The 11th Edition of the International Meeting of the SPCE-TC: Advances in Stem Cells and Cell Therapies. Frontiers Media SA, 2021. http://dx.doi.org/10.3389/978-2-88971-321-9.
Повний текст джерелаЧастини книг з теми "Tc cells"
1
Bohuslavizki, K. H., K. Röhe, H. Wolf, W. Brenner, J. U. Eberhardt, M. Schramm, M. Clausen, M. Dietel, and E. Henze. "Uptake of 4-Iodo-Doxorubicin Labelled with I-123 and Tc-99m in Tumour Cells of Gastric Carcinoma and Suprarenal Gland Carcinoma." In Radioactive Isotopes in Clinical Medicine and Research, 405–8. Basel: Birkhäuser Basel, 1995. http://dx.doi.org/10.1007/978-3-0348-7340-6_58.
Повний текст джерела
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Wen, J. G., C. Traeholt, H. W. Zandbergen, T. Morishita, and N. Koshizuka. "Direct HREM Observation of Non-unit-cell Nucleation in the Initial Stage of High Tc Superconducting Film Growth." In Advances in Superconductivity VII, 889–92. Tokyo: Springer Japan, 1995. http://dx.doi.org/10.1007/978-4-431-68535-7_200.
Повний текст джерела
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Jemmali, Mosbah, and Lotfi Bessais. "Effect of M Substitution on Structural, Magnetic and Magnetocaloric Properties of R2Fe17-x Mx (R = Gd, Nd; M = Co, Cu) Solid Solutions." In Magnetic Skyrmions. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.96299.
Повний текст джерелаАнотація:
The structure, magnetic and magnetocaloric properties of Nd2Fe17−xCox (x = 0; 1; 2; 3, 4) and Gd2Fe17-xCux (x = 0, 0.5, 1 and 1.5) solid solutions have been studied. For this purpose, these samples were prepared by arc melting and subsequent annealing at 1073 K for a 7 days. Structural analysis by Rietveld method on X-ray diffraction (XRD) have determined that these alloys crystallize in the rhombohedral Th2Zn17-type structure (Space group R¯3 m) and the substitution of iron by nickel and copper leads to a decrease in the unit cell volume. The Curie temperature (TC) of the prepared samples depends on the nickel and copper content. Based on the Arrott plot, these analyses show that Nd2Fe17-xCox exhibits a second-order ferromagnetic to paramagnetic phase transition around the Curie temperature. These curves were also used to determine the magnetic entropy change ∆SMax and the relative cooling power. For an applied field of 1.5 T, ∆SMax increase from 3.35 J/kg. K for x = 0 to 5.83 J/kg. K for x = 2. In addition the RCP increases monotonously. This is due to an important temperature range for the magnetic phase transition, contributing to a large ∆SMax shape. Gd2Fe17-xCux solid solution has a reduction of the ferromagnetic phase transition temperature from 475 K (for x = 0) to 460 K (for x = 1.5) is due to the substitution of the magnetic element (Fe) by non-magnetic atoms (Cu). The magnetocaloric effect was determined in the vicinity of the Curie temperature TC. By increasing the Cu content, an increase in the values of magnetic entropy (∆SMax) in a low applied field is observed.
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Lim, Kok Haw Jonathan, Juan W. Valle, and Wasat Mansoor. "Lung Neuroendocrine Tumours." In Oxford Textbook of Endocrinology and Diabetes 3e, edited by John A. H. Wass, Wiebke Arlt, and Robert K. Semple, 978–90. Oxford University Press, 2021. http://dx.doi.org/10.1093/med/9780198870197.003.0109.
Повний текст джерелаАнотація:
This chapter will focus on neuroendocrine tumours (NETs) of the lung and pancreas (panNETs) (non-functioning) summarizing the current understanding, diagnosis and management recommendations of these two subgroups NETs (functioning panNETs and other NET subtypes will be covered separately in subsequent chapters). Lung NETs are the most common subtype of NETs outside the gastroenteropancreatic (GEP) axis, and there has been an alarming rise in its incidence in recent years. Lung NETs are categorized into well-differentiated typical carcinoid (TC), well-differentiated atypical carcinoid (AC), poorly differentiated small cell lung carcinoma (SCLC) and poorly differentiated large cell neuroendocrine carcinoma (LCNEC); which has a significant implication on the treatment recommended. Meanwhile panNETs are classified morphologically into well-differentiated NETs (grades 1–3) and poorly differentiated NECs (grade 3). The majority of lung NETs and panNETs are non-functioning by virtue of absence of carcinoid syndrome and lack of positive serum neuroendocrine biomarkers. In both, histopathological, biochemical, and complete radiological work-up including the use of nuclear medicine imaging are pivotal in confirming the diagnosis. The last decade has witnessed rapid advances in systemic treatment options available for both lung NETs and panNETs with the advent of somatostatin analogues (SSAs), various targeted therapies, and chemotherapy; and also liver-directed therapies and other nuclear medicine ‘theranostics’. In view of the heterogeneity in NETs, each patient’s treatment pathway should be personalized, and it is recommended for all subtypes of NETs to be managed in high-volume centres in close partnership with a specialized multidisciplinary team.
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Lim, Kok Haw Jonathan, Juan W. Valle, and Wasat Mansoor. "Non-Functioning Pancreatic Neuroendocrine Tumours." In Oxford Textbook of Endocrinology and Diabetes 3e, edited by John A. H. Wass, Wiebke Arlt, and Robert K. Semple, 990–98. Oxford University Press, 2021. http://dx.doi.org/10.1093/med/9780198870197.003.0110.
Повний текст джерелаАнотація:
This chapter will focus on neuroendocrine tumours (NETs) of the lung and pancreas (panNETs) (non-functioning) summarizing the current understanding, diagnosis, and management recommendations of these two subgroups NETs (functioning panNETs and other NET subtypes will be covered separately in subsequent chapters). Lung NETs are the most common subtype of NETs outside the gastroenteropancreatic (GEP) axis, and there has been an alarming rise in its incidence in recent years. Lung NETs are categorized into well-differentiated typical carcinoid (TC), well-differentiated atypical carcinoid (AC), poorly differentiated small cell lung carcinoma (SCLC) and poorly differentiated large cell neuroendocrine carcinoma (LCNEC); which has a significant implication on the treatment recommended. Meanwhile panNETs are classified morphologically into well-differentiated NETs (grades 1-3) and poorly differentiated NECs (grade 3). The majority of lung NETs and panNETs are non-functioning by virtue of absence of carcinoid syndrome and lack of positive serum neuroendocrine biomarkers. In both, histopathological, biochemical, and complete radiological work-up including the use of nuclear medicine imaging are pivotal in confirming the diagnosis. The last decade has witnessed rapid advances in systemic treatment options available for both lung NETs and panNETs with the advent of somatostatin analogues (SSAs), various targeted therapies, and chemotherapy; and also liver-directed therapies and other nuclear medicine ‘theranostics’. In view of the heterogeneity in NETs, each patient’s treatment pathway should be personalized, and it is recommended for all subtypes of NETs to be managed in high-volume centres in close partnership with a specialized multidisciplinary team.
Тези доповідей конференцій з теми "Tc cells"
1
Ricetti, M. M., A. Samaden, V. Fregoni, M. Vigotti, F. Piovella, and E. Ascari. "TUMOR CELLS INTERACTIONS WITH SUBENDOTHELIAL EXTRACELLULAR MATRIX IN A PERFUSION SYSTEM: ROLE OF PLATELETS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643197.
Повний текст джерелаАнотація:
It has been suggested that platelets may facilitate tumor metastasis by increasing tumour cell (TC) adhesion to vascular endothelium mainly through the formation of platelet/TC aggregates. In order to further investigate this we have utilized an in vitro model combining extracellular matrices (EM) from cultured vascular endothelial cells and two neoplastic clones from a mFS6 murine fibrosarcoma: one expressing high metastatic potency (M4) and one with low metastatic potential (M9). These two sublines express an in vitro platelet aggregating activity which has previously been characterized and which correlates with in vivo metastasizing capacity. To reproduce the in vivo flow conditions a flat perfusion chamber was used (*). Glass coverslips, carrying the EM were perfused for 10’ at a wall shear rate of 450 sec™1 with reconstituted heparinized human blood containing 3×10s/ml TC, with or without platelets. Coverage of the EM with adherent TC was evaluated by a morphometric method and expressed as percent TC coverage. M4 cells adhered to EM more than did M9 cells: 4.3% TC coverage for M4, versus 2.5% TC coverage for M9. In the presence of platelets, TC adherence was greatly increased, being 9.7% for M4 and 7.8% for M9. In the experiments performed in the presence of thrombocytes no platelet/TC thrombi were found onto the EM and no TC-induced platelet aggregation was detected in blood after perfusion. Our results confirm that platelets favour the occurrence of metastasis and suggest that other mechanisms than platelet/TC aggregates formation might be involved in this process.(*) K.S. Sakarlassen et al. J.Lab.Cl in.Med. 102:522, 1983
2
Tian, Kuo, Frederik Arbeiter, Volker Heinzel, Martin Kubaschewski, Vladimir Madzharov, and Martin Mittwollen. "Current Status of the Major Cells Design of IFMIF Test Facility and Arrangement of the Cells." In 2013 21st International Conference on Nuclear Engineering. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/icone21-15991.
Повний текст джерелаАнотація:
Latest design status of the IFMIF test facility is addressed with emphasis on the function definition and key technical features of the major cells, including the test cell (TC), access cell (AC), and test module handling cells (TMHCs), as well as the arrangement of these cells. An updated specimen flow for the high flux test module (HFTM) between the TC, AC, TMHCs, and post irradiation examination facility is described and has been considered as a fundamental of the design of the cells. According to this specimen flow, the TMHC is sectioned into four dedicated hot cells and primary processing operations are allocated in these cells according to various functions, dimension of components to be handled, and contamination levels. Reinstallation of irradiated specimens in new test modules for a second or even a third irradiation campaign can also be implemented in the TMHCs. By isolating the disassembling and assembling procedures cross contamination possibility between different hot cells are minimized.
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Almirall, L., J. Aznar-Salatti, I. Calopa, A. Ordinas, and E. Bastida. "ADHESION OF TUMOR CELLS TO EXTRACELLULAR MATRIX IS MEDIATED BY FIBRONECTIN." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643207.
Повний текст джерелаАнотація:
Tumor cell (TC) vessel wall adhesion is thougth to occur at specific sites of exposed extracellular matrix (ECM).To determine the role of fibronectin (FN) in TC/ECM adhesion,we measured; 1)TC adhesion to intact cultured endothelial cell monolayers (EC) or their ECMs,with or without incubation with a polyclonal antibody (Ab) to human FN, or a monoclonal antibody (Mab) to the cell binding site of FN (3E3);2)TC adhesion to EC or their exposed ECMs incubated with specific peptides against bacteria adhesion sites (I 133-79) or the peptide GRGDSP contained in the cell binding sites of several adhesive proteins.TC adhesion was measured as number of 111In-labeled A-549 adenocarcinoma cellsxlO /disc. ECMs were exposed by removing the ECs with N2 flow or EGTA treatment.There was 5.5±1x10 A-549/ EC covered disc (Table). Treatment of ECs with the Abs or peptides had no effect on TC adhesion.TC adhesion to the two ECM preparation were 42 ± 12 and 55±10 respectively.(Table).Blockage of the FN adhesive site by either Ab inhibited TC adhesion (p < 0.01). In contrast,blockage of the bacteria-adhesion site or incubation with GRGDSP had not significant effects in TC adhesion. The table shows the results (mearv±SEM (* p < 0.01).We conclude that TC adhesion to ECM but not to ECs,is dependent upon FN.
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Bastida, E., and L. Almirall. "EFFECTS OF 13-H0DE AND HETEs ON TUMOR CELL/ENDOTHELIAL CELL INTERACTIONS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643947.
Повний текст джерелаАнотація:
We and others reported that endothelial cells (ECs) convert linoleic acid into 13-hydroxyoctadecadienoic acid (13-H0DE) under basal conditions, and arachidonic acid into 15-hydroxyeicosatet-raenoic acid (15-HETE) following stimulation (1,2). We also reported that lipoxygenase metabolism influenced platelet (PLT) interactions with ECs, tumor cells (TCs) and extracellular matrix (BM) (1,3,4). Thus, we performed studies to determine i) if TCs also produce 13-H0DE and HETEs, and ii) the effect of TC and EC 13-H0DE and HETEs synthesis on TC/EC adhesion. We measured i) the ratios of 13-H0DE:HETE in 5TC lines, under basal and stimulated conditions, in metastatic and non-metastatic TCs of the same cell line, and TCs treated with salicylate (SAL) or dipyridamole (DIP), and ii) their relationships with TC adhesion to ECs and BM. 13-H0DE and HETEs were assayed by HPLC. TC adhesion was assayed as the # radiolabelled TCs adherent to ECs or BM. cAMP was assayed by RIA. Under basal conditions, TCs produced 13-H0DE and HETEs, the intracellular ratio of which markedly affected their adhesivity; e.g. the least adhesive TC (U87MG glioblastoma) produced 21Xs more 13-H0DE than HETE’s, while a more adhesive TC (A549, adenocarcinoma) produced 4Xs more HETEs than 13-H0DE. Non-metastatic TCs preferentially produced 13-H0DE while metastatic TCs of the same cell line, produced HETEs. Stimulation of TCs or ECs decreased 13-H0DE, and increased HETE synthesis and TC/EC adhesion. Inhibiting intracellular 13-H0DE synthesis in either TCs or EC (SAL RX) enhanced TC/EC and TC/BM adhesion. Enhancing 13-H0DE synthesis by elevating cAMP (DIP RX) inhibited TC/EC and TC/BM adhesion. We conclude that 1) in vitro TCs produce 13-H0DE and HETEs, 2) the ratio of 13-H0DE:HETEs in TCs and ECs affects their adhesivity; and 3) the ratio of intracellular 13-H0DE:HETEs depends upon cAMP. This suggests that 13-H0DE:HETE ratios in TCs and ECs influence the adhesion process in the pathogenesis of thrombosis and metastasis in vivo. (1) Buchanan et al, JBC 30:1985. (2) Hopkins et al, JBC 29:1984. (3) Bastida et al, Int. J. Cane. 1987. (4) Buchanan et al, Prost. Leuk. Med., 1986.
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Kazula, Stefan, Stefanie de Graaf, and Lars Enghardt. "Review of Fuel Cell Technologies and Evaluation of their Potential and Challenges for Electrified Propulsion Systems in Commercial Aviation." In GPPS Chania22. GPPS, 2022. http://dx.doi.org/10.33737/gpps22-tc-5.
Повний текст джерелаАнотація:
This paper presents an overview of the most relevant fuel cell types and identifies the most promising options for application in propulsion systems for commercial electrified aviation. The general design, operating principles and main characteristics of polymer electrolyte membrane, alkaline, direct methanol, phosphoric acid, molten carbonate and solid oxide fuel cells are described. Evaluation criteria are derived from aviation-specific requirements for the application of fuel cells in electrified aircraft. Based on these criteria, the presented fuel cell types are evaluated by means of a weighted point rating. The results of this evaluation reveal the high potential for application of solid oxide, low temperature and high temperature polymer electrolyte membrane fuel cells. Design challenges of all fuel cell types are being emphasised, for instance, concerning cold start, cooling and supply of pressurised air.
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Szekely, V., A. Pahi, A. Poppe, M. Rencz, and A. Csendes. "SISSSI-A tool for dynamic electro-thermal simulation of analog VLSI cells." In Proceedings European Design and Test Conference. ED & TC 97. IEEE, 1997. http://dx.doi.org/10.1109/edtc.1997.582430.
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Miller, J. H., D. Nawarathna, H. Sanabria, V. Vajrala, and J. R. Claycomb. "Nonlinear Harmonic Responses of Live Cells Using High-Tc Superconducting Quantum Interference Devices." In LOW TEMPERATURE PHYSICS: 24th International Conference on Low Temperature Physics - LT24. AIP, 2006. http://dx.doi.org/10.1063/1.2355357.
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Syedain, Zeeshan H., and Robert T. Tranquillo. "Controlled Cyclic Stretching of Tissue Engineered Heart Valves: Effects on Mechanical Properties and ECM Organization." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-192583.
Повний текст джерелаАнотація:
Tissue engineering provides a means to create fully functional tissue-equivalents that can grow, repair and remodel in vivo. Our laboratory’s approach to fabricating artery- and heart valve-equivalents utilizes cell-seeded fibrin gels. However, even after 4–5 weeks of static incubation, the mechanical properties of these constructs are below those of native tissue. Previous studies in our laboratory have shown a significant role of mechanical stretching in improving properties of collagen-based tissue constructs (Isenberg and Tranquillo, 2003). We examined the effects of cyclic distention (CD) of cell-seeded fibrin-based tubular constructs (TC) and valve-equivalents (VE) after five weeks of culture. We used human dermal fibroblasts and porcine valve interstitial cells as the cell sources. Circumferential strain amplitudes from 2.5% to 15% were applied to evaluate the effects of CD on remodeling of the TC. We further hypothesized that during long-term conditioning, cells adapt to CD of constant strain amplitude, diminishing the remodeling into tissue. We tested this hypothesis by applying step-wise incremental CD (ICD) from 5%–15% strain amplitude and compared this group to a set of samples subject to CD of constant strain amplitude in this range. Based on the outcome of the cyclic distension study with tubular constructs, we applied CD to VE in a novel bioreactor.
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Jiang, Siya, Cheng Tian, and Song Fu. "Evolution of unsteady vortex structures and rotating stall cells in a centrifugal compressor with vaneless diffuser." In GPPS Xi'an21. GPPS, 2022. http://dx.doi.org/10.33737/gpps21-tc-152.
Повний текст джерелаАнотація:
The rotating stall in a centrifugal compressor with a vaneless diffuser is investigated using the IDDES (Improved Delayed Detached Eddy Simulation) method. The flow fields from design to off-design conditions are assessed for comparison. The characteristic frequency of the rotating stall is accurately predicted, illustrated by both the pressure spectra and the velocity signals. This low-frequency peak corresponds to the stall cells, induced by different vortex structures in the impeller passages and the diffuser. Compared with the flow fields of the rotating instability and the near design conditions, a unique flow structure of the rotating stall condition is the longitudinal vortices upstream of the impeller inlet, connecting to the tornado-type vortices in one or two consecutive passages. In the downstream passages, the large-scale vortices on the shroud side cause the blockage. The deformed vortices stretching along streamlines towards the outlet are dominant in the diffuser. Through the high fidelity numerical method, physical mechanisms are analysed in detail to give a new perspective of the rotating stall in a centrifugal compressor.
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Pakalapati, Mano Rahul K., Ahmed Kadhim, Susan K. Earles, and Mano Varun K. Pakalapati. "A novel method for generating a low temperature coefficient (TC) reference voltage from high TC reference cells to achieve wide temperature stability for remote sensors." In 2017 IEEE Sensors Applications Symposium (SAS). IEEE, 2017. http://dx.doi.org/10.1109/sas.2017.7894116.
Повний текст джерелаЗвіти організацій з теми "Tc cells"
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Alessa, Mohammed, Tayba Wahedi, Jumanah Alsairafi, Nouf Almatrafi, Wisal Shuaib, Johara Alnafie, Fatimah Alzubaidi, and Soha Elmorsy. Prevalence of Thyroid cancer in Saudi Arabis: Systematic review and Meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, September 2022. http://dx.doi.org/10.37766/inplasy2022.9.0088.
Повний текст джерелаАнотація:
Review question / Objective: What is the prevalence of Thyroid cancer among population in kingdom of Saudi Arabia?. The aim of this systematic review is to scrutinize the prevalence of thyroid cancer (TC) in Saudi Arabia and assess the relative frequency of subgroups related to types of thyroid cancer, age, and gender. Condition being studied: Thyroid cancer is an abnormal growth of cells that starts in the thyroid gland. There is four types of differentiated thyroid cancer, three of these cancer develop from the follicular cells, the papillary thyroid cancer, follicular thyroid cancer, Hürthle cell carcinoma, and one rare type develops from the thyroid’s C cells called medullary thyroid cancer. There is one undifferentiated thyroid cancer called anaplastic thyroid cancer.
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Kane, S., and P. Zhou. A Portable Cell Maintenance System for Rapid Toxicity Monitoring Final Report CRADA No. TC-02081-04. Office of Scientific and Technical Information (OSTI), September 2017. http://dx.doi.org/10.2172/1399750.
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Kane, S. A Portable Cell Maintenance System for Rapid Tocicity Monitoring Final Report CRADA No. TC-02081-04. Office of Scientific and Technical Information (OSTI), November 2006. http://dx.doi.org/10.2172/919893.
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Cooper, J. F., and H. D. Haley. Research and Development of Zinc Air Fuel Cell To Achieve Commercialization Final Report CRADA No. TC-1544-98. Office of Scientific and Technical Information (OSTI), September 2017. http://dx.doi.org/10.2172/1399730.
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Dederer, J. T., and L. W. Hrubesh. Advanced Alumina Aerogel Insulation for Application in Solid Oxide Fuel Cell Power Generation Systems Final Report CRADA No. TC-1373-96. Office of Scientific and Technical Information (OSTI), February 2018. http://dx.doi.org/10.2172/1424646.
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Hrubesh, L. Advanced Alumina Aerogel Insulation for Application in Solid Oxide Fuel Cell Power Generation Systems Final Report CRADA No. TC-1373-96. Office of Scientific and Technical Information (OSTI), February 2018. http://dx.doi.org/10.2172/756380.
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Tokarz, F. J., J. F. Cooper, and D. Haley. Commercialization of LLNL Zinc Air Fuel Cell Technology For Stationary And Mobile Applications And Electromechanical Battery For Mobile Applications Final Report CRADA No. TC-1420-97. Office of Scientific and Technical Information (OSTI), November 2017. http://dx.doi.org/10.2172/1408984.
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Liu, Zhanjiang John, Rex Dunham, and Boaz Moav. Developmental and Evaluation of Advanced Expression Vectors with Both Enhanced Integration and Stable Expression for Transgenic Farmed Fish. United States Department of Agriculture, December 2001. http://dx.doi.org/10.32747/2001.7585196.bard.
Повний текст джерелаАнотація:
The objectives of the project were to develop expression vectors using the Sleeping Beauty transposon technology and the genetic border elements to provide both enhanced integration rate and stable transgene expression, and to evaluate the application of such vectors in farmed fish such as catfish and carp. The panel recommended adding the objective of evaluating the endogenous transposable elements, particularly in catfish, in order to evaluate the applicability of the expression vectors while reduc1ng efforts in real production of transgenic fish considering the focus of the project was to develop the vector and evaluation of its applicability, not producing transgenic fish. Efficient production of transgenic farmed fish is hindered by two major problems: mosaicism due to delayed integration after single-cell stage, and silencing of transgene expression. In this project, we proposed to combat these problems by coupling the Sleeping Beauty transposon technology that can enhance integration rate and the border elements that can insulate transgene from position effect. Our major objective was to develop a new generation of expression vector that contains both of these elements. We have developed expression vectors containing both the Sleeping Beauty transposon signals, inverted repeats and direct repeats (IR and DR, respectively), and the border elements, scs and scs'. Growth hormone minigene has been cloned into this vector for applications of such vectors in growth enhancement. Luc reporter gene has been also cloned into this vector cascades for relative easy evaluation of transgene expression. Transgenic fish have been produced using these expression vectors in both catfish (US) and carp (Israel). Much effort was also devoted to evaluation of the endogenous transposable elements in catfish as recommended by the BARD grant panel. Multiple families of Tcl-like transposons were identified from catfish. Surprisingly, many Tc I-related transcripts were identified. Among these transcripts, both the sense and antisense transcripts were present. Some of the transcripts may be useful for development of novel transposase-based technology for aquaculture applications in the future. This project has both scientific and aquaculture implications. First, to develop expression vectors containing both IR/DR and scs/scs' repeated elements have been reported being extremely technically difficult due to excision of the repeated sequences by the E. coli host during cloning processes. We have successfully constructed this advanced vector that contained very complex cascades for both gene integration and gene regulation. We have produced transgenic fish using such vectors. This advanced expression vector should be useful for production of transgenic fish. By simply replacing the growth hormone gene, any gene of interest can be readily inserted in this vector. Thus this vector should provide technological possibility for early integration and stable expression of any economically important genes in aquaculture. We have also evaluated the applications of the Sleeping Beauty-based vectors in terms of the impact of gene size and found that the size of trans gene drastically affects transposition. The system will be only useful for transferring genes smaller than 5.6 kb. We have also identified novel transposase-related transcripts that may be useful for the development of novel transposase-based technologies for general scientific research and for aquaculture applications.