Готові списки джерел за темами / Targetted capture

Добірка наукової літератури з теми "Targetted capture"

Автор: Grafiati
Опубліковано: 10 грудня 2022
Оновлено: 28 січня 2023

Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями

Оберіть тип джерела:

Зміст

Ознайомтеся зі списками актуальних статей, книг, дисертацій, тез та інших наукових джерел на тему "Targetted capture".

Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.

Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.

Статті в журналах з теми "Targetted capture"

1

Scobie, Martin, and Michael D. Threadgill. "Tumour-targetted boranes. Part 3. Synthesis of carbamate-linked nitroimidazolyl carboranes designed for boron neutron capture therapy of cancer." Journal of the Chemical Society, Perkin Transactions 1, no. 15 (1994): 2059. http://dx.doi.org/10.1039/p19940002059.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
2

SCOBIE, M., and M. D. THREADGILL. "ChemInform Abstract: Tumor-Targetted Boranes. Part 3. Synthesis of Carbamate-Linked Nitroimidazolyl Carboranes Designed for Boron Neutron Capture Therapy of Cancer." ChemInform 26, no. 1 (August 18, 2010): no. http://dx.doi.org/10.1002/chin.199501175.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
3

He, Jin, Lei Li, Yan Wang, and Xindong Wu. "Hierarchical features-based targeted aspect extraction from online reviews." Intelligent Data Analysis 25, no. 1 (January 26, 2021): 205–23. http://dx.doi.org/10.3233/ida-194952.

Повний текст джерела
Анотація:
With the prevalence of online review websites, large-scale data promote the necessity of focused analysis. This task aims to capture the information that is highly relevant to a specific aspect. However, the broad scope of the aspects of the various products makes this task overarching but challenging. A commonly used solution is to modify the topic models with additional information to capture the features for a specific aspect (referred to as a targeted aspect). However, the existing topic models, either perform the full analysis to capture features as many as possible or estimate the similarity to capture features as coherent as possible, overlook the fine-grained semantic relations between the features, resulting in the captured features coarse and confusing. In this paper, we propose a novel Hierarchical Features-based Topic Model (HFTM) to extract targeted aspects from online reviews, then to capture the aspect-specific features. Specifically, our model can not only capture the direct features posing target-to-feature semantics but also capture the latent features posing feature-to-feature semantics. The experiments conducted on real-world datasets demonstrate that HFTMl outperforms the state-of-the-art baselines in terms of both aspect extraction and document classification.
Стилі APA, Harvard, Vancouver, ISO та ін.
4

Doud, Carl W., Gerrit W. Cuperus, Phillip Kenkel, Mark E. Payton, and Thomas W. Phillips. "Trapping Tribolium castaneum (Coleoptera: Tenebrionidae) and Other Beetles in Flourmills: Evaluating Fumigation Efficacy and Estimating Population Density." Insects 12, no. 2 (February 7, 2021): 144. http://dx.doi.org/10.3390/insects12020144.

Повний текст джерела
Анотація:
This paper reports beetle pests common to flourmills targeted during a series of trapping studies over a two-year period in flourmill 1 and a one year period in flourmill 2. Objectives were (1) use pheromone-baited traps to detect T. castaneum (Herbst) and other pest species present for their distribution over space and time, (2) monitor T. castaneum activity before and after fumigations to assess efficacy of the treatment, and (3) correlate counts of T. castaneum via trap capture against direct T. castaneum counts from samples of the milled flour to assess the value of trap data to estimate relative size of the pest population. Traps were deployed in two different flourmills over two consecutive years. T. castaneum was the most commonly trapped beetle during both years in mill 1. In mill 2, Typhaea stercorea (L.) and Cryptolestes ferrugineus (Stephens) were both captured in higher numbers than T. castaneum. In mill 1, trap capture was higher overall during Year 2 for most of the species compared with capture during Year 1, likely due to a dust cover modification made for the pitfall trap used in Year 2. Trap capture was also evaluated by location within the mills and a significant difference was found in the capture of T. stercorea during both years in mill 1. T. castaneum captures were significantly reduced following most fumigations, which used methyl bromide in milling areas and phosphine in bulk-stored finished flour. However, in most cases trap catches showed that beetle populations were not eliminated. Trap captures after fumigation suggest either that the fumigations were not entirely effective, or that full grown adult beetles were entering the mill soon after fumigation. When captures of T. castaneum from traps in two spaces of mill 1 during Year 2 were compared with counts of beetles from samples of siftings collected in the finished flour, the correlation coefficients were nearly significant for both sets of traps.
Стилі APA, Harvard, Vancouver, ISO та ін.
5

D’Alessio, Stewart J., Lisa Stolzenberg, and Dustin Dariano. "Does Targeted Capture Reduce Terrorism?" Studies in Conflict & Terrorism 37, no. 10 (September 15, 2014): 881–94. http://dx.doi.org/10.1080/1057610x.2014.941437.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
6

Olfert, Melissa, Rebecca Hagedorn, Makenzie Barr, Oluremi Famodu, Jessica Rubino, and Jade White. "eB4CAST: An Evidence-Based Tool to Promote Dissemination and Implementation in Community-Based, Public Health Research." International Journal of Environmental Research and Public Health 15, no. 10 (September 29, 2018): 2142. http://dx.doi.org/10.3390/ijerph15102142.

Повний текст джерела
Анотація:
eB4CAST, evidence-Based forecast C-capture, A-assemble, S-sustain, T-timelessness (eB4CAST), framework was developed from existing dissemination and implementation (D & I) constructs as a dissemination tool to promote community-based program usability and future application in targeted populations. eB4CAST captures and transforms research findings into a dissemination report that shows program need and impact to endorse program continuation and expansion. This is achieved through direct and indirect data collection of community factors and program impact that can showcase the need for program sustainability and potential for future dissemination sites. Testimonials, individual feedback, and program process and outcomes contribute to the direct data while data collected from census, county, and state databases and reports allow for indirect information to be captured and analyzed. Capturing data in the two levels allow eB4CAST to forecast program need and highlight program impact through a footprint. eB4CAST framework for dissemination tool creation is organized into four sections: Capture, Assemble, Sustainability, and Timelessness. Capture encompasses the collection of indirect and direct data related to intervention goals. Assemble is the compilation of the data into a visually appealing and easily understood media. Sustainability encourages the use of dissemination tools to provide forecast of program need and footprint of program impact back to community participants, program leaders, and key stakeholders to endorse program sustainability. Lastly, timelessness encourages cyclic movement through these constructs to continue program monitoring and data sharing to ensure timeless program evaluation and conformation to change in needs. The eB4CAST framework provides a systematic method to capture justification of program need and impact of community-based research that can be modified to fit diverse public health interventions providing a necessary D & I tool.
Стилі APA, Harvard, Vancouver, ISO та ін.
7

Lasa, Rodrigo, Laura Navarro-de-la-Fuente, Anne C. Gschaedler-Mathis, Manuel R. Kirchmayr, and Trevor Williams. "Yeast Species, Strains, and Growth Media Mediate Attraction of Drosophila suzukii (Diptera: Drosophilidae)." Insects 10, no. 8 (July 31, 2019): 228. http://dx.doi.org/10.3390/insects10080228.

Повний текст джерела
Анотація:
Specific ecological interactions between insects and microbes have potential in the development of targeted pest monitoring or control techniques for the spotted wing drosophilid, Drosophila suzukii (Matsumura), an exotic invasive pest of soft fruit. To evaluate D. suzukii attraction to yeast species from preferred types of fruit, three yeasts were isolated from blackberry fruit and two yeasts from raspberry fruit and used to bait simple plastic bottle traps. Saccharomyces cerevisiae and Hanseniaspora uvarum were identified from blackberries, whereas a different H. uvarum strain was identified from raspberry. Yeast identification was based on sequence analysis of the D1/D2 domain of the large subunit 26S rRNA gene. Commercial baker’s yeast (S. cerevisiae) was similar or more effective for the capture of D. suzukii males and females than yeasts isolated from blackberry or raspberry when grown in sucrose. However, when grown in corn syrup, a strain of S. cerevisiae from blackberry captured the highest number of females and a strain of H. uvarum isolated from raspberry captured high numbers of males and females. Species of Candida, Hanseniaspora, and Pichia from a laboratory yeast collection did not outperform baker’s yeast in pairwise tests when grown in sucrose solution or yeast-peptone-dextrose medium. The raspberry strain of H. uvarum grown in corn syrup outperformed S. cerevisiae grown in sucrose, in terms of captures in baited traps under laboratory conditions. We conclude that yeast species, strain, and growth medium can have a marked influence on D. suzukii attraction to baited traps, a finding that could assist in the development of yeast-related monitoring or control techniques targeted at this pest.
Стилі APA, Harvard, Vancouver, ISO та ін.
8

Wylie, Todd N., Kristine M. Wylie, Brandi N. Herter, and Gregory A. Storch. "Enhanced virome sequencing using targeted sequence capture." Genome Research 25, no. 12 (September 22, 2015): 1910–20. http://dx.doi.org/10.1101/gr.191049.115.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
9

Jones, Matthew R., and Jeffrey M. Good. "Targeted capture in evolutionary and ecological genomics." Molecular Ecology 25, no. 1 (July 30, 2015): 185–202. http://dx.doi.org/10.1111/mec.13304.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
10

Chou, Lan-Szu, C.-S. Jonathan Liu, Benjamin Boese, Xinmin Zhang, and Rong Mao. "DNA Sequence Capture and Enrichment by Microarray Followed by Next-Generation Sequencing for Targeted Resequencing: Neurofibromatosis Type 1 Gene as a Model." Clinical Chemistry 56, no. 1 (January 1, 2010): 62–72. http://dx.doi.org/10.1373/clinchem.2009.132639.

Повний текст джерела
Анотація:
Abstract Background: The introduction and use of next-generation sequencing (NGS) techniques have taken genomic research into a new era; however, implementing such powerful techniques in diagnostics laboratories for applications such as resequencing of targeted disease genes requires attention to technical issues, including sequencing template enrichment, management of massive data, and high interference by homologous sequences. Methods: In this study, we investigated a process for enriching DNA samples that uses a customized high-density oligonucleotide microarray to enrich a targeted 280-kb region of the NF1 (neurofibromin 1) gene. The captured DNA was sequenced with the Roche/454 GS FLX system. Two NF1 samples (CN1 and CN2) with known genotypes were tested with this protocol. Results: Targeted microarray capture may also capture sequences from nontargeted regions in the genome. The capture specificity estimated for the targeted NF1 region was approximately 60%. The de novo Alu insertion was partially detected in sample CN1 by additional de novo assembly with 50% base-match stringency; the single-base deletion in sample CN2 was successfully detected by reference mapping. Interferences by pseudogene sequences were removed by means of dual-mode reference-mapping analysis, which reduced the risk of generating false-positive data. The risk of generating false-negative data was minimized with higher sequence coverage (>30×). Conclusions: We used a clinically relevant complex genomic target to evaluate a microarray-based sample-enrichment process and an NGS instrument for clinical resequencing purposes. The results allowed us to develop a systematic data-analysis strategy and algorithm to fit potential clinical applications.
Стилі APA, Harvard, Vancouver, ISO та ін.
Більше джерел

Дисертації з теми "Targetted capture"

1

Chong, Lauren Camille. "Detection of genomic rearrangements in archival lymphoma tissues using targeted capture sequencing." Thesis, University of British Columbia, 2016. http://hdl.handle.net/2429/57716.

Повний текст джерела
Анотація:
The B-cell lymphomas are a heterogeneous group of disease entities arising from mature B lymphocytes and are characterized by frequent genomic rearrangements. Recurrent rearrangements involving the MHC class II transactivator CIITA and the programmed death 1 ligands PDL1 and PDL2 have been shown to contribute to an immune privilege phenotype in multiple B-cell lymphomas, with implications for novel therapeutic approaches. However, the landscape of fusion partners for these genes has not been well characterized and methods that utilize formalin-fixed paraffin-embedded (FFPE) tumour samples for breakpoint discovery have not been explored. We selected 68 B-cell lymphoma patients with known CIITA and PDL1/2 rearrangement status determined by fluorescence in situ hybridization (FISH) break-apart assays. DNA surrounding the CIITA and PDL1/2 loci was captured from FFPE tumour libraries using a hybridization-based target enrichment assay and sequenced on an Illumina HiSeq 2500. Multiple structural variant (SV) detection tools were used in an ensemble approach to generate SV predictions. We identified 35 novel translocation partners and observed translocation cluster breakpoint regions (CBRs) in CIITA, PDL1, PDL2 and the SOCS1 tumour suppressor gene downstream of CIITA. Recurrent intrachromosomal deletions, inversions and duplications were also identified in each region. Immunohistochemistry (IHC) analysis of PD-L1 and PD-L2 surface expression demonstrated that CBR translocations and a subset of intra-chromosomal rearrangements are significantly associated with increased protein expression of the respective ligand. In conjunction with published reports this suggests that distinct rearrangement types have variable functional consequences. We also report many SVs below the detection resolution of FISH, suggesting the value of a combined approach integrating FISH, capture sequencing and IHC data for characterizing genomic rearrangements in lymphomas. This study confirms the utility of a targeted sequencing approach for detecting structural variation in FFPE lymphoma tissues. Future capture designs interrogating the full set of recurrently rearranged lymphoma genes are being explored with the aim of designing a comprehensive, high-throughput and clinically relevant assay for routine profiling of rearrangement status to guide clinical decision making.
Science, Faculty of
Graduate
Стилі APA, Harvard, Vancouver, ISO та ін.
2

Beck, J. H. "Efficient targeted optimisation for the design of pressure swing adsorption systems for CO2 capture in power plants." Thesis, University College London (University of London), 2014. http://discovery.ucl.ac.uk/1429983/.

Повний текст джерела
Анотація:
Pressure swing adsorption (PSA) is a cyclic adsorption process for gas separation and purification, and can be used in a variety of industrial applications, for example, hydrogen purification and dehydration. PSA is, due to its low operational cost and its ability to efficiently separate CO2 from flue gas, a promising candidate for post-combustion carbon capture in power plants, which is an important link in the Carbon Capture and Storage technology chain. PSA offers many design possibilities, but to optimise the performance of a PSA system over a wide range of design choices, by experimental means, is typically too costly, in terms of time and resources required. To address this challenge, computer experiments are used to emulate the real system and to predict the performance. The system of PDAEs that describes the PSA process behaviour is however typically computationally expensive to simulate, especially as the cyclic steady state condition has to be met. Over the past decade, significant progress has been made in computational strategies for PSA design, but more efficient optimisation procedures are needed. One popular class of optimisation methods are the Evolutionary algorithms (EAs). EAs are however less efficient for computationally expensive models. The use of surrogate models in optimisation is an exciting research direction that allows the strengths of EAs to be used for expensive models. A surrogate based optimisation (SBO) procedure is here developed for the design of PSA systems. The procedure is applicable for constrained and multi-objective optimisation. This SBO procedure relies on Kriging, a popular surrogate model, and is used with EAs. The main application of this work is the design of PSA systems for CO2 capture. A 2- bed/6-step PSA system for CO2 separation is used as an example. The cycle configuration used is sufficiently complex to provide a challenging, multi-criteria example.
Стилі APA, Harvard, Vancouver, ISO та ін.
3

Abbasinejad, Enger Shirin. "Dosimetry Studies of Different Radiotherapy Applications using Monte Carlo Radiation Transport Calculations." Doctoral thesis, Uppsala University, Department of Oncology, Radiology and Clinical Immunology, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-9277.

Повний текст джерела
Анотація:

Developing radiation delivery systems for optimisation of absorbed dose to the target without normal tissue toxicity requires advanced calculations for transport of radiation. In this thesis absorbed dose and fluence in different radiotherapy applications were calculated by using Monte Carlo (MC) simulations.

In paper I-III external neutron activation of gadolinium (Gd) for intravascular brachytherapy (GdNCB) and tumour therapy (GdNCT) was investigated. MC codes MCNP and GEANT4 were compared. MCNP was chosen for neutron capture reaction calculations. Gd neutron capture reaction includes both very short range (Auger electrons) and long range (IC electrons and gamma) products. In GdNCB the high-energetic gamma gives an almost flat absorbed dose delivery pattern, up to 4 mm around the stent. Dose distribution at the edges and inside the stent may prevent stent edge and in-stent restenosis. For GdNCT the absorbed dose from prompt gamma will dominate over the dose from IC and Auger electrons in an in vivo situation. The absorbed dose from IC electrons will enhance the total absorbed dose in the tumours and contribute to the cell killing.

In paper IV a model for calculation of inter-cluster cross-fire radiation dose from β-emitting radionuclides in a breast cancer model was developed. GEANT4 was used for obtaining absorbed dose. The dose internally in cells binding the isotope (self-dose) increased with decreasing β-energy except for the radionuclides with substantial amounts of conversion electrons and Auger electrons. An effective therapy approach may be a combination of radionuclides where the high self-dose from nuclides with low β-energy should be combined with the inter-cell cluster cross-fire dose from high energy β-particles.

In paper V MC simulations using correlated sampling together with importance sampling were used to calculate spectra perturbations in detector volumes caused by the detector silicon chip and its encapsulation. Penelope and EGSnrc were used and yielded similar results. The low energy part of the electron spectrum increased but to a less extent if the silicon detector was encapsulated in low z-materials.

Стилі APA, Harvard, Vancouver, ISO та ін.
4

Gasc, Cyrielle. "Capture de gènes par hybridation couplée au séquençage de nouvelle génération pour l'exploration d'échantillons métagénomiques. : Génomique et écologie microbienne." Thesis, Clermont-Ferrand 1, 2016. http://www.theses.fr/2016CLF1MM24.

Повний текст джерела
Анотація:
Les microorganismes représentent la forme de vie la plus diverse et abondante sur Terre et jouent un rôle fondamental dans tous les processus biologiques. Cependant, du fait de la grande diversité des communautés microbiennes, la caractérisation fine des environnements complexes reste difficile par les approches moléculaires actuelles de PCR et de métagénomique. En effet, ces approches ne conduisent qu’à une caractérisation partielle des communautés et ne permettent pas systématiquement d’associer la structure des communautés aux fonctions métaboliques réalisées. L’approche de capture de gènes par hybridation appliquée à des échantillons métagénomiques complexes a démontré son intérêt pour révéler toute la diversité connue mais aussi inconnue des biomarqueurs fonctionnels ciblés, ainsi que pour enrichir leurs régions flanquantes sur quelques centaines de permettant en évidence des associations de gènes. Ainsi, les travaux de thèse ont visé à développer une nouvelle méthode de capture de gènes par hybridation capable d’enrichir de façon ciblée de larges régions génomiques à partir d’échantillons complexes, permettant ainsi de faire le lien entre structure et fonction des communautés microbiennes. Ces développements ont nécessité la détermination de sondes de capture, l’utilisation d’une méthode d’extraction d’ADN de haut poids moléculaire et la mise au point d’un protocole de capture permettant de piéger des fragments nucléiques de grande taille (jusqu’à 50 kb). La validation de la méthode de capture par hybridation sur un échantillon environnemental de sol a permis de révéler tout son potentiel. Appliquée au gène exprimant l’ARNr 16S, cette stratégie a permis de révéler une diversité microbienne non accessible par les approches moléculaires conventionnelles, avec une résolution d’identification jusqu'au niveau de l’espèce rendue possible grâce à la reconstruction de la séquence complète de ce marqueur phylogénétique. Appliquée à un gène fonctionnel, elle a conduit à la reconstruction de la séquence du biomarqueur et de ses régions flanquantes pouvant atteindre plusieurs dizaines de kb, permettant d’identifier les microorganismes possédant les capacités métaboliques d’intérêt. Ainsi, la capture par hybridation représente une approche alternative prometteuse pour le diagnostic environnemental en conduisant à une meilleure caractérisation des communautés microbiennes
Microorganisms are the most diverse and abundant life forms on Earth and are key players in thefunctioning of all biological processes. Nevertheless, PCR and metagenomics strategies aiming to describemicrobial communities are hampered by their huge diversity. Indeed, these molecular methods only drive to apartial description of communities and do not systematically allow linking functions back to the identities of themicroorganisms. Hybridization capture applied to complex metagenomic samples has demonstrated its efficiency to reveal all known and unknown diversity of targeted biomarkers, and to enrich their flanking regions over a few hundred bp facilitating the discovery of gene associations.Thus, this work aimed at developing a new hybridization capture method capable of specifically enrichinglarge genomic regions from complex samples allowing to associate structure and functions of communities. Thedevelopment of this method required the design of capture probes, the use of a high molecular weight DNAextraction method, and the elaboration of a capture protocol dedicated to the enrichment of large genomicfragments (up to 50 kbp).The validation of the hybridization capture method on an environmental soil sample uncovered all itspotential. Applied to the 16S rRNA gene, this strategy revealed greater microbial diversity than conventionalmolecular methods and improved phylogenetic resolution up to the species level thanks to the reconstruction offull-length genes. Applied to a functional gene, the method enabled the reconstruction of large genomic regionscarrying the targeted biomarker and its flanking regions over several tens of kbp, leading to the identification ofmicroorganisms with specific metabolic functions. Hybridization capture thus appears as a promising alternativemethod for environmental diagnosis, through providing a better knowledge of microbial communities
Стилі APA, Harvard, Vancouver, ISO та ін.
5

Zhao, Xiaobin. "Targeting CD37 and folate receptor for cancer therapy strategies based on engineered protein and liposomes /." Columbus, Ohio : Ohio State University, 2007. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1174678307.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
6

Polak, Roberta. "Desenvolvimento e fabricação de filmes ultra-­finos, obtidos pela técnica layer-by-layer, para aplicações na entrega direcionada de fármacos e na captura seletiva de bio-­marcadores." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/9/9135/tde-27042015-152718/.

Повний текст джерела
Анотація:
O objetivo geral deste trabalho foi explorar a versatilidade de filmes multicamadas de polieletrólitos (PEM) e suas aplicações em sistemas de entrega de drogas e como filmes funcionais para aplicações biomédicas. Filmes PEM montados pela técnica de camada por camada (layer­-by­-layer, LbL), foram explorados em três aplicações principais. Na primeira, foi explorado o desenvolvimento de um protocolo de funcionalização em filmes de poli(alilamina)/poli (estireno sulfonato), PAH/SPS. Os parâmetros de construção do filme para biotinilação dos grupamentos amina do PAH foram otimizados e aplicados na captura e detecção do antígeno específico da próstata (PSA), na concentração de 100 a 0,1 ng/mL, usando pontos quânticos (Qdots). Em comparação com outros trabalhos, este sistema apresentou uma boa sensibilidade na detecção de PSA, dentro do limite de detecção clínica de 0,4 a 0,1 ng/mL. A segunda aplicação envolveu o desenvolvimento de filmes de sacrifício baseados nas interações naturais da mucina submandibular bovina e da lectina, jacalina (BSM/JAC). Filmes de BSM/JAC apresentaram estabilidade quando submetidos a uma ampla faixa de pH (pH 3-­-9) e em solução de alta força iônica (5 M NaCl). A dissolução dos filmes BSM/JAC pôde ser seletivamente desencadeada mediante à incubação em solução de melibiose, 37 °C, pH 7,4, sem apresentar citotoxicidade às células. Na última parte deste trabalho, a incorporação de lipossomos ecogênicos (ELIP) em mochilas celulares foi investigada. Mochilas celulares são \"patches\" de 7­-10 µm de diâmetro que podem ser fabricados por meio de deposição alternada de polímeros utilizando-­-se a técnica de LbL, sobre uma matriz pré­-moldada obtida por fotolitografia, a fim de criar um sistema composto por três multicamadas estratificadas: uma região de liberação, para promover o destacamento do substrato, uma região de carga de droga, e uma região adesiva às células. O uso de ELIP permitiu incorporação de até 9x mais doxorrubicina (DOX) se comparado com o fármaco livre em solução absorvido pelos dos filmes. A liberação de DOX pelos filmes foi monitorado por 25 dias. Mochilas contendo ELIP-­DOX foram então aderidos a monócitos, e sua viabilidade monitorados por 72h. Mochilas vazias mostraram diminuir a proliferação de monócitos ao longo das 72 horas, enquanto mochilas carregadas com ELIP-­DOX mostraram uma diminuição dramática na população celular, apontando uma potencialização dos efeitos da droga pela sua proximidade com as células.
The overall goal of this thesis was to exploit the versatility of polyelectrolite multilayers (PEM) to be applied in drug delivery systems and biofunctionalizable films for biomedical applications. PEM films assembled by the layer-by­-layer technique were explored in three main applications. In the first part of this work, the development of a functionalization protocol of poly(allylamine)/poly(styrene sulfonate), PAH/SPS was explored. The optimal film parameters to the use of biotinylated multilayers were applied for the capture and detection of prostate specific antigen (PSA) protein in the range of 100 to 0.1 ng/mL, by using quantum dots. Compared to previous work, this system presented a good sensitivity for PSA detection that is within the clinical limit range of 0.4 to 0.1 ng/mL. The second application involved the creation of a novel sacrificial multilayer film. Films based in natural interactions of bovine submaxillary mucin and the lectin jacalin, BSM/JAC were assembled. BSM/JAC films showed stability when underwent a wide rage of pH (pH 3 to 9) and high ionic strength (5 M NaCl) solutions. BSM/JAC dissolution could be triggered released by incubation in melibiose at 37 °C in pH 7.4 buffer, without cytotoxicity. In the last part of this work the incorporation of echogenic liposomes (ELIP) into cell backpacks was investigated. Cell backpacks are 7-10 µm diameter patches that can be fabricated through LbL polymer deposition onto a photopatterned array to create a stacked composite of three stratified multilayer systems: a releasable region for easy detachment from the substrate, a drug payload region, and a cell adhesive region. The use of ELIP allowed up to 9x more doxorubicin (DOX) loading when compared to free drug in solution adsorbed through the films. DOX release from films was monitored for over 25 days. ELIP­-DOX backpacks were then attached to mouse monocytes and their viability monitored by 72h. Empty backpacks showed to decrease monocytes proliferation over the course of 72h, while ELIP­-DOX backpacks showed a dramatic decrease in cell population, showing that DOX effects were enhancement in drug potency by its proximity.
Стилі APA, Harvard, Vancouver, ISO та ін.
7

Christmas, Matthew John. "Assessing genomic variation in the hopbush, Dodonaea viscosa, to investigate micro-evolution and adaptation." Thesis, 2016. http://hdl.handle.net/2440/119801.

Повний текст джерела
Анотація:
In this thesis I use a range of genomic tools in order to investigate aspects of the evolutionary history of the hopbush, Dodonaea viscosa (L.) Jacq. (hopbush). Understanding the past evolutionary processes that have led to the distribution and adaptation of contemporary populations can help to inform on how populations may continue to adapt into the future. A changing climate is altering selection pressures and populations will need to adapt to these if they are to persist. I pay consideration to how plant populations may adapt to a changing climate, as well as what constrains adaptive responses and how an understanding of these constraints can inform conservation management, in a review article (Chapter 2). Dodonaea viscosa is globally widespread and distributed across the Australian continent where it originated. Within Australia there are seven taxonomically identified subspecies, but subspecies boundaries are not clear. Here, I analysed genome-wide single nucleotide polymorphic (SNP) markers for each of the seven subspecies using genetic structure analysis as well as network-based and Bayesian-based methods to assess phylogenetic relationships and show that defining subspecies for D. viscosa based on morphology is not wholly accurate or useful (chapter three). A consideration of a population’s geographic location, environment and Pleistocene history appears to better explain the distribution of genetic variation across this species, rather than the presence of distinct gene pools corresponding to morphologically defined subspecies. The thesis then moves onto an examination of the population history and signatures of selection in the narrow-leaf hopbush, Dodonaea viscosa ssp. angustissima along an environmental gradient within South Australia. Firstly, I generated a transcriptome reference genome by sequencing RNA from several individuals and used a range of bioinformatic tools to assemble and annotate it (chapter four). This transcriptome was then used to selectively target a subset of 970 genes via ‘hybrid-capture target enrichment’ in order to develop a set of SNP markers present within putatively functional genes distributed throughout the genome. The SNP markers were used to characterise population genomic metrics of the target populations, such as genetic diversity and structure (chapter five). A range of analytical methods was used, including Principal Component Analysis, Mantel tests, AMOVA, and an FST outlier analysis algorithm. The data demonstrates there to be three distinct genetic clusters across the study region with low gene flow among them. The potential origins of these clusters are discussed, along with the implications for restoration practice using germplasm derived from these distinct gene pools. SNP markers within the sequenced target genes were also analysed for signatures of selection, providing evidence for local adaptation driven by climatic factors (chapter six). A combination of FST outlier analysis and genotype-environment association analyses found 74 SNPs showing strong evidence for selection. Genes containing these SNPs under environmental selection were diverse, including aquaporin and abscisic acid (ABA) genes, as well as genes with ontologies relating to environmental responses, such as ‘response to water deprivation’. Selection acting on these populations has led to clines in allele frequencies in a number of functional genes, including some genes associated with leaf shape and stomatal characteristics, the phenotypes for which have been observed previously to vary along this environmental gradient. The implications for conservation and restoration practice using such data are further discussed. The thesis concludes with a consideration of the future directions of research that can be informed by and further strengthen the findings of this thesis. Confirmation of adaptive significance of the genomic variation identified here is required. The use of common garden and reciprocal transplant experiments in order to provide evidence for links between genotype and phenotype and to asses the relative roles of genetic adaptation and plasticity in this species are discussed.
Thesis (Ph.D.) (Research by Publication) -- University of Adelaide, School of Biological Sciences, 2016.
Стилі APA, Harvard, Vancouver, ISO та ін.
8

Liang, JingXin. "Interrogating the Structural Landscape of Malaria Biomarkers with Epitope Targeted Peptide Capture Agents." Thesis, 2018. https://thesis.library.caltech.edu/11069/12/JingXinLiang_FullThesis_June2018.pdf.

Повний текст джерела
Анотація:

Antibodies have conventionally been used as molecular recognition agents against epitopes, or antigenic regions, for protein capture and detection. The ability of monoclonal and polyclonal antibodies to selectively bind their targets with high affinities makes them excellent agents for specific protein recognition. However, as large proteins themselves (~150 kDa), antibodies are susceptible to changes in pH, temperature, and biochemical environment, particularly proteolytic cleavage. Additionally, epitope binding on antibodies is reliant on their rigid tertiary structure to position key functional groups that facilitation antigen recognition. Retaining the integrity of the protein structure creates rigid limitations against chemical modifications of antibodies to suit unique needs.

Protein-catalyzed capture agents (PCCs) developed within the Heath group at Caltech address the limitation of antibodies as affinity agents. Using epitope-targeted in situ click screening methodology, the Heath group has developed peptidomimetic molecules that offer an alternative solution to antibodies. These PCCs exhibit high affinity and selectivity for their protein targets. As peptide-based molecules, PCCs can be engineered to be biochemically stable and resistant to changes in their chemical environment. Their peptide-based structures are readily amenable to chemical modifications and allow for adaptation to a range of applications.

This thesis describes the development of PCCs against unique protein biomarkers for the detection of the most lethal species of malaria infection, Plasmodium falciparum. Malaria is a global health epidemic and its eradication is reliant on rapid and accurate diagnostics for prompt treatment. We targeted the P. falciparum specific biomarkers lactate dehydrogenase (LDH) and Histidine-rich protein 2 (HRP2), both of which present unique challenges for protein capture. The LDH biomarker is homologous across malaria species, whereas HRP2 is highly polymorphic and lacks distinct secondary structure. The variation in sensitivity of HRP2 detection by antibody-based tests has been attributed to the genetic polymorphism of the biomarker.

In Chapter 1, we describe the development of high affinity PCCs that bind selectively to the LDH biomarker. We targeted an epitope that was highly homologous across LDH species. This chapter also details the expansion of mono-valent PCC agents into bivalent ligands using the protein architecture to select secondary ligands for binding improvement. For the HRP2 biomarker, we developed a multiple epitope targeting strategy to address protein polymorphism. We targeted for epitopes in HRP2 and developed PCCs that bind in the range of monoclonal antibodies.

Chapter 2 details the expansion of PCC agents developed against HRP2 into multivalent molecules for improved binding. The development of bivalent ligands from combinatorial screening of linker libraries is presented. The optimal linker lengths determined by the screens are described.

In Chapter 3, a general strategy for targeting the protein landscape to inhibit formation of a protein and biomolecule complex with PCCs against HRP2 is demonstrated. Specifically, the inhibition of heme sequestration by HRP2 is shown. A bivalent ligand that targets two epitopes on HRP2 is shown to have enhanced inhibitory potency over any single or cocktail combination of PCCs.

Altogether, the studies herein demonstrate the utility of peptidomimetic molecules as agents for protein capture and detection as well as a generalizable strategy of functional inhibition through epitope-targeting.

Стилі APA, Harvard, Vancouver, ISO та ін.
9

Huang, Lin-Chiang Sherlock, and 黃麟強. "Targeted Drug Delivery System Design and Development for Boron Neutron Capture Therapy on Cancer Treatment." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/71545701228827769976.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
10

Kao, Yu-Che, and 高譽哲. "Synthesis and Characterization of Targeted Gold Nanocage-decorated Magnetic Nanocarrier for Simultaneous Capture and Photothermal/photodynamic Ablation of Circulating Tumor Cells." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/24411947021569038905.

Повний текст джерела
Анотація:
碩士
國立交通大學
材料科學與工程學系奈米科技碩博士班
104
Metastasis is associated with 90 % of cancer-related deaths and circulating tumor cells (CTCs) play an important role as an intermediate during the metastatic invasion. Targeting CTCs when they are transiting in the blood circulation could disrupt cancer dissemination and reduce the probability of cancer death. Herein, we introduce a magnetic photosensitive nanocarrier, gold nanocages-conjugated thiol-magnetic nanocarriers (GTMNCs) to trap and destroy cancer cells in situ in flow. Gold nanocages (AuNCs) are able to induce hyperthermia which results in cancer cells necrosis under 808 nm irradiation and sensitize singlet oxygen (1O2) formation to activate apoptosis program upon NIR illumination (940 nm LED, 915 nm laser). The temperature of the suspension of GTMNCs raised to more than 50 oC under 808 nm laser light. 1O2 phosphorescence emission and singlet oxygen sensor green (SOSG) fluorescent probe were used to confirm the 1O2 generation upon NIR illumination. As GTMNCs were functionalized with anti-epithelial cell adhesion molecule (EpCAM) antibody to specifically target mouse breast cancer cell (4T1), in vitro experiments demonstrated 9-fold increase in intracellular ROS levels by quantifying SOSG and dihydroethidium (DHE) fluorescence. Scavengers were also used as negative control. Cytotoxicity assay indicates that GTMNCs are high biocompatibility in dark until photoirradiation for performing photothermal therapy (PTT) and photodynamic therapy (PDT). Further magnetic enrichment of 4T1 cells and simultaneous photodestruction in the microfluidic channel using the GTMNCs proved a potential methodology of phototherapy for CTCs removal.
Стилі APA, Harvard, Vancouver, ISO та ін.

Книги з теми "Targetted capture"

1

Li, Chenhong, and Gavin J. P. Naylor. Targeted Gene Capture for Phylogenetic Systematics and Population Genetics. Taylor & Francis Group, 2018.

Знайти повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
2

Li, Chenhong, and Gavin J. P. Naylor. Targeted Gene Capture for Phylogenetic Systematics and Population Genetics. Taylor & Francis Group, 2018.

Знайти повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
3

Locke, Joseph. Introduction. Oxford University Press, 2017. http://dx.doi.org/10.1093/acprof:oso/9780190216283.003.0001.

Повний текст джерела
Анотація:
The introduction presents the book’s major argument that, through the politics of prohibition, ambitious evangelical leaders were able to inject themselves into southern life and create the political conditions that would later identify the American South as the Bible Belt. H. L. Mencken coined that term later, in the 1920s, to capture what he saw as the South’s peculiar alliance of region and religion, but the reality that Mencken described was only the closing chapter of a long historical process. To explore that process, the introduction establishes key concepts such as clericalism and anticlericalism, argues for Texas as the proper focus for a targeted study, and previews major characters and milestones in the development of a politicized brand of southern religion.
Стилі APA, Harvard, Vancouver, ISO та ін.
4

David, Kretzmer. Part 3 The Post 9/11-Era (2001–), 57 US Extra-Territorial Actions Against Individuals: Bin Laden, Al Awlaki, and Abu Khattalah—2011 and 2014. Oxford University Press, 2018. http://dx.doi.org/10.1093/law/9780198784357.003.0057.

Повний текст джерела
Анотація:
This chapter discusses the targeted killing by the US of Osama bin Laden in Pakistan and of Anwar al Awlaki in Yemen, and the capture of Ahmed Abu Kattalah in Libya. It presents the facts and context of the actions, the legal position of the US and other protagonists and reactions in the international community. It proceeds to discuss the arguments for and against the legality of these extra-territorial actions by the US under law regarding use of force (but not under ius in bello). In the final section it is argued that rather than having precedential value the actions and reactions in these cases serve to emphasize the wide gap that exists in the different perceptions of states and scholars regarding the law on the extra-territorial use of force against terrorist groups or other groups of non-state actors.
Стилі APA, Harvard, Vancouver, ISO та ін.
5

Berry, Justin, Youssef Chouhoud, and Jane Junn. Reaching Beyond Low-Hanging Fruit. Edited by Lonna Rae Atkeson and R. Michael Alvarez. Oxford University Press, 2016. http://dx.doi.org/10.1093/oxfordhb/9780190213299.013.1.

Повний текст джерела
Анотація:
This chapter argues for the use of creative and targeted strategies rather than a one-size-fits-all approach to capturing information on low-incidence populations. It first conceptualizes low-incidence populations and highlights the existing empirical literature on them and then frames the challenges of polling these populations with an overview of sampling, contacting, and analytical strategies. It highlights the inherent trade-offs of each approach and points to the factors to consider when determining which strategy is best suited to particular research questions. It then details polling efforts designed to capture attitudes and behaviors of American Muslims, Asian Americans, and nonelected political activists. The chapter concludes with a discussion of fruitful polling practices for conducting research on low-incidence U.S. populations, arguing that the approach to polling these populations must be equally informed by the unique characteristics of the target group and the analytical conclusions one seeks to draw.
Стилі APA, Harvard, Vancouver, ISO та ін.
6

Markwica, Robin. The Logic of Affect. Oxford University Press, 2018. http://dx.doi.org/10.1093/oso/9780198794349.003.0002.

Повний текст джерела
Анотація:
Chapter 2 develops the logic of affect, or emotional choice theory, as an alternative action model besides the traditional logics of consequences and appropriateness. Drawing on research in psychology and sociology, the model captures not only the social nature of emotions but also their bodily and dynamic character. It posits that the interplay between identities, norms, and five key emotions—fear, anger, hope, pride, and humiliation—can shape decision-making in profound ways. The chapter derives a series of propositions how these five key emotions tend to influence the choice behavior of political leaders whose countries are targeted by coercive diplomacy. These propositions specify the affective conditions under which target leaders are likely to accept or reject a coercer’s demands. Even when emotions produce powerful impulses, humans will not necessarily act on them, however. The chapter thus also incorporates decision-makers’ limited ability to regulate their emotions into the logic of affect.
Стилі APA, Harvard, Vancouver, ISO та ін.
7

Shires, James. The Politics of Cybersecurity in the Middle East. Oxford University Press, 2022. http://dx.doi.org/10.1093/oso/9780197619964.001.0001.

Повний текст джерела
Анотація:
Cybersecurity is a complex and contested issue in international politics. By focusing on "great powers"--the US, the EU, Russia and China--studies in the field often fail to capture the specific politics of cybersecurity in the Middle East, especially in Egypt and the GCC states. For these countries, cybersecurity policies and practices are entangled with those of long-standing allies in the US and Europe, and are built on reciprocal flows of data, capital, technology and expertise. At the same time, these states have authoritarian systems of governance more reminiscent of Russia or China, including approaches to digital technologies centred on sovereignty and surveillance. This book is a pioneering examination of the politics of cybersecurity in the Middle East. Drawing on new interviews and original fieldwork, James Shires shows how the label of cybersecurity is repurposed by states, companies and other organisations to encompass a variety of concepts, including state conflict, targeted spyware, domestic information controls, and foreign interference through leaks and disinformation. These shifting meanings shape key technological systems as well as the social relations underpinning digital development. But however the term is interpreted, it is clear that cybersecurity is an integral aspect of the region's contemporary politics.
Стилі APA, Harvard, Vancouver, ISO та ін.
8

Strawser, Bradley Jay, Ryan Jenkins, and Michael Robillard, eds. Who Should Die? Oxford University Press, 2017. http://dx.doi.org/10.1093/oso/9780190495657.001.0001.

Повний текст джерела
Анотація:
This academic text brings together, in one volume, the most recent and innovative accounts of liability to harm in war. The concept of liability has become a crucial wedge issue within the military ethics community, as the claim that combatants are morally equal has come under withering criticism. Scholars have been exploring the various causal factors that underlie a person’s liability to be intentionally targeted with potentially lethal violence—such as her culpable contribution to an unjust threat. These new categories of liability cut across the old equality of combatants, suggesting that not all soldiers are equally liable to harm, and that even civilians can be liable to harm. This text offers a “who’s who” of contemporary scholars working on and rigorously debating the major ethical questions surrounding killing in war, including liability to harm, rights theory, self-defense, selective conscientious objection, obligations toward civilians, and autonomous weapons. This volume collects, expands upon, and provides new and updated analyses of these concepts that have yet to be captured in a single work. As a convenient and authoritative collection of such discussions, this title is uniquely and well suited for university-level teaching and as a scholarly reference for ethicists, policymakers, and other stakeholders.
Стилі APA, Harvard, Vancouver, ISO та ін.
9

Gaiha, Raghav, Raghbendra Jha, Vani S. Kulkarni, and Nidhi Kaicker. Diets, Nutrition, and Poverty. Edited by Ronald J. Herring. Oxford University Press, 2014. http://dx.doi.org/10.1093/oxfordhb/9780195397772.013.029.

Повний текст джерела
Анотація:
This chapter addresses a persistent tension in current debates over food security, with illustrative data from India. The case allows us to disaggregate concepts in food policy that are often lumped together, so as to better understand what is at stake in rapidly changing economies more generally. Despite rising incomes, there has been sustained decline in per capita nutrient intake in India in recent years. The assertion by Deaton and Dreze (2009) that poverty and undernutrition are unrelated is critically examined. A demand-based model in which food prices and expenditure played significant roles proved robust, while allowing for lower calorie “requirements” due to less strenuous activity patterns, life-style changes, and improvements in the epidemiological environment. This analysis provides reasons for not delinking nutrition and poverty; it confirms the existence of poverty-nutrition traps in which undernutrition perpetuates poverty. A new measure of child undernutrition that allows for multiple anthropometric failures (e.g., wasting, underweight, and stunting) points to much higher levels of undernutrition than conventional ones. Dietary changes over time, and their nutritional implications, have welfare implications at both ends of the income and social-status pyramids. Since poverty is multidimensional, money-metric indicators such as minimum income or expenditure are not reliable, because these cannot adequately capture all the dimensions. The emergent shift of the disease burden toward predominately food-related noncommunicable diseases (NCDs) poses an additional challenge. Finally, the complexity of normative issues in food policy is explored. Current approaches to food security have veered toward a “right-to-food” approach. There are, however, considerable problems with creating appropriate mechanisms for effectuating that right; these are explored briefly. Cash transfers touted to avoid administrative costs and corruption involved in rural employment guarantee and targeted food-distribution programs are likely to be much less effective if the objective is to enable large segments of the rural population to break out of nutrition-poverty traps. The chapter ends by exploring an alternative model, based on the same normative principle: a “right to policies,” or a “right to a right.”
Стилі APA, Harvard, Vancouver, ISO та ін.

Частини книг з теми "Targetted capture"

1

Zhigulev, Artemy, and Pelin Sahlén. "Targeted Chromosome Conformation Capture (HiCap)." In Spatial Genome Organization, 75–94. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2497-5_5.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
2

Larsson, Börje, and Nigel E. A. Crompton. "Monochromatic X-Rays, Slow Neutrons, and Radionuclides for Targeted Radiotherapy." In Frontiers in Neutron Capture Therapy, 5–12. Boston, MA: Springer US, 2001. http://dx.doi.org/10.1007/978-1-4615-1285-1_1.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
3

Ribière, Céline, Réjane Beugnot, Nicolas Parisot, Cyrielle Gasc, Clémence Defois, Jérémie Denonfoux, Delphine Boucher, Eric Peyretaillade, and Pierre Peyret. "Targeted Gene Capture by Hybridization to Illuminate Ecosystem Functioning." In Microbial Environmental Genomics (MEG), 167–82. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3369-3_10.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
4

Connor, Rebecca E., Simona G. Codreanu, Lawrence J. Marnett, and Daniel C. Liebler. "Targeted Protein Capture for Analysis of Electrophile-Protein Adducts." In Methods in Molecular Biology, 163–76. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-321-3_15.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
5

Comtet-Marre, Sophie, Oshma Chakoory, and Pierre Peyret. "Targeted 16S rRNA Gene Capture by Hybridization and Bioinformatic Analysis." In Microbial Environmental Genomics (MEG), 187–208. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2871-3_10.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
6

Cantsilieris, Stuart, Holly A. Stessman, Jay Shendure, and Evan E. Eichler. "Targeted Capture and High-Throughput Sequencing Using Molecular Inversion Probes (MIPs)." In Methods in Molecular Biology, 95–106. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6442-0_6.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
7

Pröll, Johannes, Carina Fischer, Gabriele Michelitsch, Martin Danzer, and Norbert Niklas. "High-Throughput Sequencing of the Major Histocompatibility Complex following Targeted Sequence Capture." In Methods in Molecular Biology, 87–112. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6750-6_5.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
8

Ray, Paresh Chandra, Sadia Afrin Khan, Anant Kumar Singh, Dulal Senapati, Zhen Fan, Teresa Demeritte, and Rajashekhar Kanchanapally. "Bioconjugated Gold Nanoparticle for Rapid Capture and Targeted Photothermal Lysis of Pathogenic Bacteria." In ACS Symposium Series, 107–28. Washington, DC: American Chemical Society, 2012. http://dx.doi.org/10.1021/bk-2012-1119.ch005.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
9

Crona, Joakim, Peter StÃ¥lberg, Per Hellman, and Peyman Björklund. "Comprehensive genetic screening of pheochromocytoma and paraganglioma through targeted capture and next generation sequencing." In The Endocrine Society's 95th Annual Meeting and Expo, June 15–18, 2013 - San Francisco, MON—42—MON—42. 2055 L Street NW, Suite 600, Washington, DC 20036: The Endocrine Society, 2013. http://dx.doi.org/10.1210/endo-meetings.2013.ahpaa.11.mon-42.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
10

Lavoie, R. Ashton, Taufika Islam Williams, R. Kevin Blackburn, Ruben G. Carbonell, and Stefano Menegatti. "Development of Peptide Ligands for Targeted Capture of Host Cell Proteins from Cell Culture Production Harvests." In Methods in Molecular Biology, 489–506. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1186-9_31.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.

Тези доповідей конференцій з теми "Targetted capture"

1

"Novel aptamer-based targeted agents for boron neutron capture therapy." In Bioinformatics of Genome Regulation and Structure/Systems Biology (BGRS/SB-2022) :. Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, 2022. http://dx.doi.org/10.18699/sbb-2022-476.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
2

White, Briggs M., Robin W. Ames, and Patcharin Burke. "Conditions in Advanced Turbines for IGCC Power Plants With Carbon Capture." In ASME Turbo Expo 2013: Turbine Technical Conference and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/gt2013-94609.

Повний текст джерела
Анотація:
The anticipated conditions in advanced turbines for Integrated Gasification Combined Cycle (IGCC) power plants with Carbon Capture (CC) are discussed to provide researchers with accurate boundary conditions to conduct targeted research and development activities. Information was collected through reviews of existing IGCC plant conditions, system studies, permitting requirements, and industry feedback. Anticipated bulk gas phase characteristics are provided, including temperatures, pressures, mass flow rates, and compositions. Particulate loadings and sizing information are also presented; however, compositional information is limited.
Стилі APA, Harvard, Vancouver, ISO та ін.
3

Ranjan, Shashi, and Yong Zhang. "Capture of Circulating Tumor Cells (CTCs) Using a Novel Micro-Device." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53030.

Повний текст джерела
Анотація:
Circulating tumor cells (CTCs) are metastatic cells that are detached from a tumor and are found in the blood of cancer patients. The presence of CTCs in the blood indicates the metastatic stage of cancer, so the detection of CTCs has been considered as a diagnosis and prognosis marker. The number of CTCs in blood has been found to be related with survival-rate. Also, CTCs have spurred interest for understanding the biology of these cells by performing genetic-level analysis which can be helpful in the development of targeted cancer-therapy or in drug-selection.
Стилі APA, Harvard, Vancouver, ISO та ін.
4

Gray, PN, W. Guo, JL Dickens, and A. Kammesheidt. "Targeted Resequencing of Genes Implicated in PCD Using Sequence Capture and Next-Generation Sequencing." In American Thoracic Society 2009 International Conference, May 15-20, 2009 • San Diego, California. American Thoracic Society, 2009. http://dx.doi.org/10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a3675.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
5

Chmielecki, Juliann, Martin Peifer, Agnes Viale, Nicolas Socci, Peilin Jia, Zhongming Zhao, Roman Thomas, and William Pao. "Abstract LB-236: Identifying novel kinase fusions via targeted DNA capture and next-generation sequencing." In Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.am2011-lb-236.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
6

Dekemele, Kevin, Patrick Van Torre, and Mia Loccufier. "Nonlinear Energy Sink and Targeted Energy Transfer in Smart Structures." In ASME 2022 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2022. http://dx.doi.org/10.1115/detc2022-89608.

Повний текст джерела
Анотація:
Abstract Nonlinear energy sinks (NESs) are strongly nonlinear mechanical oscillators that are weakly coupled to a mechanical primary system. Through targeted energy transfer (TET), vibration energy is irreversibly transferred from the primary system to the NES, making the NES an attractive passive vibration control device. Furthermore, because of the NES’ strong nonlinearity, the NES has a variable natural frequency, unlike linear vibration control devices. This enables a self-tuning property where the NES adapts to the primary system’s vibration frequency, making the NES a broadband vibration absorber. This self-tuning extends to multi-frequency vibrations through resonance capture cascade (RCC), where the NES tunes itself to the different frequencies in sequential order. Recently, some works have discussed the piezoelectric NES, a nonlinear electrical circuit that interfaces with a mechanical system through a piezoelectric transducer. This paper will show for the first time that by using an electromagnetic transducer instead of a piezoelectrical transducer, an electromagnetic NES is obtained. Furthermore, this work will show that dynamics of mechanical, piezoelectric and electromagnetic NESs can be generalized under a universal NES in a slow flow expression and that resonance capture cascade occurs for all the NES types. The performance of the NES configurations are compared and their physical components are discussed.
Стилі APA, Harvard, Vancouver, ISO та ін.
7

Wullems a, Christian, Geoff Dell b, and Yvonne Toft b. "Improving the Railway’s Understanding of Accident Causation Through an Integrated Approach to Human Factors Analysis and Technical Safety Data Recording." In Applied Human Factors and Ergonomics Conference. AHFE International, 2021. http://dx.doi.org/10.54941/ahfe100653.

Повний текст джерела
Анотація:
This paper describes a safety data recording and analysis system that has been developed to capture safety occurrences including precursors using high-definition forward-facing video from train cabs and data from other train-borne systems. The paper describes the data processing model and how events detected through data analysis are related to an underlying socio-technical model of accident causation. The integrated approach to safety data recording and analysis insures systemic factors that condition, influence or potentially contribute to an occurrence are captured both for safety occurrences and precursor events, providing a rich tapestry of antecedent causal factors that can significantly improve learning around accident causation. This can ultimately provide benefit to railways through the development of targeted and more effective countermeasures, better risk models and more effective use and prioritization of safety funds. Level crossing occurrences are a key focus in this paper with data analysis scenarios describing causal factors around near-miss occurrences. The paper concludes with a discussion on how the system can also be applied to other types of railway safety occurrences.
Стилі APA, Harvard, Vancouver, ISO та ін.
8

Allahyar, Amin, Mark Pieterse, Joost Swennenhuis, Tjitske Los-de Vries, Mehmet Yilmaz, Roos Leguit, Ruud Meijers, et al. "Abstract PO-45: Robust detection of translocations in lymphoma FFPE samples using Targeted Locus Capture-based sequencing." In Abstracts: AACR Virtual Meeting: Advances in Malignant Lymphoma; August 17-19, 2020. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/2643-3249.lymphoma20-po-45.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
9

Walker, Brian Andrew, Aarif Ahsan, Parvathi Sudha, Tasneem Kausar, Sushmita Rane, Erin Flynt, and Anjan Thakurta. "Abstract 450: A targeted capture panel to identify translocations, copy number abnormalities, and mutations in multiple myeloma." In Proceedings: AACR Annual Meeting 2021; April 10-15, 2021 and May 17-21, 2021; Philadelphia, PA. American Association for Cancer Research, 2021. http://dx.doi.org/10.1158/1538-7445.am2021-450.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
10

Roh, Byeong-Min, Soundar R. T. Kumara, Hui Yang, Timothy W. Simpson, Paul Witherell, and Yan Lu. "In-Situ Observation Selection for Quality Management in Metal Additive Manufacturing." In ASME 2021 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2021. http://dx.doi.org/10.1115/detc2021-70035.

Повний текст джерела
Анотація:
Abstract Metal additive manufacturing (MAM) provides a larger design space with accompanying manufacturability than traditional manufacturing. Recently, much research has focused on simulating the MAM process with regards to part geometry, porosity, and microstructure properties. Despite continued advances, MAM processes have many variables that are not well understood with respect to their effect on the part quality. With the common use of in-situ sensors — such as CMOS cameras and infrared cameras — numerous, real-time datasets can be captured and analyzed for monitoring both the process and the part. However, currently, real-time data predominantly focuses on the build failure and process anomalies by capturing the printing defects (cracks/peel-off). A large amount of data — such as melt pool geometries and temperature gradients — are just beginning to be explored, along with their connections to final part quality. Towards investigating these connections, in this paper we propose models that capture numerous sensor capabilities and associate them with the corresponding, real-time, physical phenomena. These sensor models lay the foundation for a comprehensive, knowledge framework that forms the basis for quality monitoring and management of MAM process outcomes. Using our previously developed process ontology model [1–3], which describes the relationship between process variables and process outcomes, we can discover the relationship between the real-time, physical phenomena and the deviations in the targeted, build quality. For example, statistically significant sensor data that predicts deviations from targeted process qualities can be detected and used to control the process parameters. Case studies that scope the physical phenomena and sensor data are provided for verifying the effectiveness and efficiency of the proposed qualification and certification models.
Стилі APA, Harvard, Vancouver, ISO та ін.

Звіти організацій з теми "Targetted capture"

1

Alatas, Vivi, Abhijit Banerjee, Rema Hanna, Benjamin Olken, Ririn Purnamasari, and Matthew Wai-Poi. Does Elite Capture Matter? Local Elites and Targeted Welfare Programs in Indonesia. Cambridge, MA: National Bureau of Economic Research, February 2013. http://dx.doi.org/10.3386/w18798.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
2

Chen, Liming, David Raitzer, Rana Hasan, Rouselle Lavado, and Orlee Velarde. What Works to Control COVID-19? Econometric Analysis of a Cross-Country Panel. Asian Development Bank, December 2020. http://dx.doi.org/10.22617/wps200354-2.

Повний текст джерела
Анотація:
The paper examines the effects of nonpharmaceutical interventions on transmission of the novel coronavirus disease (COVID-19) as captured by its reproduction rate 𝑅t. Using cross-country panel data, the paper finds that while lockdown measures have strong effects on 𝑅t, gathering bans appear to be more effective than workplace and school closures. Ramping up the testing and tracing of COVID-19 cases is found to be especially effective in controlling the spread of the disease where there is greater coverage of paid sick leave benefits. Workplace and school closures are found to have large negative effects on gross domestic product compared with other measures, suggesting that a more targeted approach can be taken to keep the epidemic controlled at lower cost.
Стилі APA, Harvard, Vancouver, ISO та ін.
3

Stewart, Alastair, and Miranda Morgan. A Final Evaluation of Oxfam's Gendered Enterprise and Markets Programme (2014-18): Summary of findings. Oxfam GB, December 2019. http://dx.doi.org/10.21201/2019.5358.

Повний текст джерела
Анотація:
Gendered Enterprise and Markets (GEM) is Oxfam GB’s approach to market systems development. The GEM approach facilitates change in market systems and social norms, with the aim of ensuring more sustainable livelihood opportunities for marginalized women and men. The GEM DFID AidMatch Programme (June 2014–February 2018) worked within the soya, milk and vegetable value chains targeting women smallholder farmers in areas of poverty. The programme aimed to benefit 63,600 people (10,600 smallholder households) living in Zambia, Tajikistan and Bangladesh through increases in household income, women having greater influence over key livelihood decisions within their households and communities, and engaging in livelihoods more resilient to shocks, such as natural disasters and market volatility. This evaluation was designed to investigate if and how the GEM programme contributed to its intended outcomes – not only in the lives of individual women smallholder farmers targeted by the programme but also in terms of changes in their communities and the larger market system. It also sought to capture any potential unintended outcomes of the programme. This summary report outlines the key findings from the three individual country evaluations in Bangladesh, Tajikistan and Zambia - for which the full reports are also available.
Стилі APA, Harvard, Vancouver, ISO та ін.
4

Tillett, Will, and Oliver Jones. Improving Rural Sanitation in Challenging Contexts. Institute of Development Studies (IDS), October 2021. http://dx.doi.org/10.19088/slh.2021.020.

Повний текст джерела
Анотація:
Of the two billion people worldwide lacking access to at least basic sanitation, seven out of ten live in rural areas. Progress has been made on increasing rural sanitation and access levels are rising, but barriers remain in reaching the ‘last mile’ or some 10 to 20 per cent of the population who live in the most challenging contexts. The factors affecting the ability of households to construct and use toilets, as well as the challenges sanitation programmes face in reaching specific groups, are highly diverse. Applying one-size fits all approaches has been proven not to work; therefore, we need more nuanced, adapted, and targeted approaches to capture the universality element of the Sustainable Development Goals (SDGs) and ensure that no one is left behind. However, we recognise that challenges can be persistent and there are limited documented examples of how to overcome these challenges at scale. The Sanitation Learning Hub, UNICEF, and WaterAid commissioned this study to map rural sanitation approaches in challenging contexts and the guidance currently being used, drawing out emerging experiences and lessons. It involved key informant interviews (KIIs) with 44 interviewees, and consulting over 180 documented resources. This Learning Brief provides an overview of the study findings.
Стилі APA, Harvard, Vancouver, ISO та ін.
5

Tillett, Will, and Oliver Jones. ‘Improving Rural Sanitation in Challenging Contexts’ Sanitation Learning Hub Learning Brief 8. The Sanitation Learning Hub, Institute of Development Studies, March 2021. http://dx.doi.org/10.19088/slh.2021.006.

Повний текст джерела
Анотація:
Of the two billion people worldwide lacking access to at least basic sanitation, seven out of ten live in rural areas. Progress has been made on increasing rural sanitation and access levels are rising, but barriers remain in reaching the ‘last mile’ or some 10 to 20 per cent of the population who live in the most challenging contexts. The factors affecting the ability of households to construct and use toilets, as well as the challenges sanitation programmes face in reaching specific groups, are highly diverse. Applying one-size fits all approaches has been proven not to work; therefore, we need more nuanced, adapted, and targeted approaches to capture the universality element of the Sustainable Development Goals (SDGs) and ensure that no one is left behind. However, we recognise that challenges can be persistent and there are limited documented examples of how to overcome these challenges at scale. The Sanitation Learning Hub, UNICEF, and WaterAid commissioned this study to map rural sanitation approaches in challenging contexts and the guidance currently being used, drawing out emerging experiences and lessons. It involved key informant interviews (KIIs) with 44 interviewees, and consulting over 180 documented resources. This Learning Brief provides an overview of the study findings.
Стилі APA, Harvard, Vancouver, ISO та ін.
6

Morgan, Miranda, and Alastair Stewart. Making Market Systems Work for Women Farmers in Tajikistan: A final evaluation of Oxfam's Gendered Enterprise and Markets programme in Tajikistan. Oxfam GB, December 2019. http://dx.doi.org/10.21201/2019.5372.

Повний текст джерела
Анотація:
Gendered Enterprise and Markets (GEM) is Oxfam GB’s approach to market systems development. The GEM approach facilitates change in market systems and social norms, with the aim of ensuring more sustainable livelihood opportunities for marginalized women and men. The GEM DFID AidMatch Programme (June 2014–February 2018) worked within the soya, milk and vegetable value chains targeting women smallholder farmers in areas of poverty. The programme aimed to benefit 63,600 people (10,600 smallholder households) living in Zambia, Tajikistan and Bangladesh through increases in household income, women having greater influence over key livelihood decisions within their households and communities, and engaging in livelihoods more resilient to shocks, such as natural disasters and market volatility. In Tajikistan, the Gendered Enterprise and Markets (GEM) programme has been implemented in five districts of Khatlon Province by Oxfam in partnership with local public organizations, League of Women Lawyers of Tajikistan (LWL) and Neksigol Mushovir. The GEM programme in Tajikistan sought to directly improve the livelihoods of an estimated 3,000 smallholder farmers (60 percent women) in fruit and vegetable value chains through improved production skills, resilience to climate risks, access to market opportunities and greater engagement with market players, and strengthened ability to influence private sector and government actors. The evaluation was designed to investigate if and how the GEM programme might have contributed to its intended outcomes – not only in the lives of individual women smallholder farmers targeted by the programme but also to changes in their communities and the larger market system. It also sought to capture any potential unintended outcomes of the programme.
Стилі APA, Harvard, Vancouver, ISO та ін.
7

Savani, Manu, and Alastair Stewart. Making Market Systems Work for Women Dairy Farmers in Bangladesh: A final evaluation of Oxfam's Gendered Enterprise and Markets programme in Bangladesh. Oxfam GB, December 2019. http://dx.doi.org/10.21201/2019.5365.

Повний текст джерела
Анотація:
Gendered Enterprise and Markets (GEM) is Oxfam GB’s approach to market systems development. The GEM approach facilitates change in market systems and social norms, with the aim of ensuring more sustainable livelihood opportunities for marginalized women and men. The GEM DFID AidMatch Programme (June 2014–February 2018) worked within the soya, milk and vegetable value chains targeting women smallholder farmers in areas of poverty. The programme aimed to benefit 63,600 people (10,600 smallholder households) living in Zambia, Tajikistan and Bangladesh through increases in household income, women having greater influence over key livelihood decisions within their households and communities, and engaging in livelihoods more resilient to shocks, such as natural disasters and market volatility. The GEM programme in Bangladesh was implemented under Oxfam Bangladesh’s flagship REE-CALL programme (Resilience, through Economic Empowerment, Climate Adaptation, Leadership and Learning). GEM operated in seven districts across Bangladesh, with the project activities implemented by seven local partners. The project aimed to establish 84 producer groups for smallholder dairy farmers, and this was achieved during the first year. Building on these local networks, GEM aimed to deliver a suite of training and support covering assertiveness, rights and leadership skills, agricultural practice and disaster risk management. The evaluation was designed to investigate if and how the GEM programme might have contributed to its intended outcomes – not only in the lives of individual women smallholder farmers targeted by the programme but also in changes in their communities and the larger market system. It also sought to capture any potential unintended outcomes of the programme.
Стилі APA, Harvard, Vancouver, ISO та ін.
8

Morgan, Miranda, Alastair Stewart, and Simone Lombardini. Making Market Systems Work for Women Farmers in Zambia: A final evaluation of Oxfam's Gendered Enterprise and Markets programme in the Copperbelt region of Zambia. Oxfam GB, December 2019. http://dx.doi.org/10.21201/2019.5389.

Повний текст джерела
Анотація:
Gendered Enterprise and Markets (GEM) is Oxfam GB’s approach to market systems development. The GEM approach facilitates change in market systems and social norms, with the aim of ensuring more sustainable livelihood opportunities for marginalized women and men. The GEM DFID AidMatch Programme (June 2014–February 2018) worked within the soya, milk and vegetable value chains targeting women smallholder farmers in areas of poverty. The programme aimed to benefit 63,600 people (10,600 smallholder households) living in Zambia, Tajikistan and Bangladesh through increases in household income, women having greater influence over key livelihood decisions within their households and communities, and engaging in livelihoods more resilient to shocks, such as natural disasters and market volatility. In Zambia, the GEM programme has been implemented in four districts of the Copperbelt Province in coordination with implementing partners Heifer Programmes International and the Sustainable Agricultural Programme (SAP). The GEM programme in the Copperbelt seeks to directly improve the livelihoods of an estimated 4,000 smallholder farmers (75 percent women) in the dairy and soya value chains through improved production skills, resilience to climate risks, access to market opportunities, greater engagement with market players and strengthened ability to influence private sector and government actors. The evaluation was designed to investigate if and how the GEM programme might have contributed to its intended outcomes – not only in the lives of individual women smallholder farmers targeted by the programme but also to changes in their communities and the larger market system. It also sought to capture any potential unintended outcomes of the programme.
Стилі APA, Harvard, Vancouver, ISO та ін.
9

Saillant, Eric, Jason Lemus, and James Franks. Culture of Lobotes surinamensis (Tripletail). Mississippi Department of Marine Resources, January 2014. http://dx.doi.org/10.18785/ose.001.

Повний текст джерела
Анотація:
The Tripletail, Lobotes surinamensis, is a pelagic fish found in tropical and sub-tropical waters of all oceans. Tripletails are often associated with floating debris and make frequent incursions in bays and estuaries where they are targeted by recreational fishermen. In Mississippi waters the species is typically present during the late spring and summer season that also correspond to the period of sexual maturation and spawning (Brown-Peterson and Franks 2001). Tripletail is appreciated as a gamefish but is also prized for its flesh of superior quality. The fast growth rate of juveniles in captivity documented by Franks et al. (2001) and the excellent quality of Tripletail flesh both contribute to the potential of this species for marine aquaculture. In addition, the production of cultured juveniles would be precious to develop a better understanding of the biology, early life history and habitat use of Tripletail larvae and juveniles, a topic largely undocumented to date, through experimental releases and controlled studies. The culture of tripletail thus supports the Tidelands Trust Fund Program through improved conservation of natural resources, potential enhancement of fisheries productivity and potential development of a new economic activity on the Gulf coast producing tripletail via aquaculture. The Objective of this project was to initiate development of methods and techniques needed to spawn captive held tripletail broodfish and raise their offspring to evaluate their growth and development in captivity. In this report we will present the results of studies aiming to develop methods and protocols for captive spawning of tripletail and the first data obtained on the early development of tripletail larvae. A major issue that was encountered with tripletail broodstock development during the project lied in the difficulties associated with identifying the sex of adults caught in the wild and candidates for being incorporated in mating sets for spawning. This issue was addressed during the course of the project by examining the potential of a non-lethal method of hormonal sexing. The results of these preliminary investigations are presented in the third part of this report. All protocols used in the project were determined with the guidance of the Institutional Animal Care and Use Committee (IACUC) of the University of Southern Mississippi (USM IACUC protocol number 10100108).
Стилі APA, Harvard, Vancouver, ISO та ін.
10

Hollingsworth, Hilary, Debbie Wong, Elizabeth Cassity, Prue Anderson, and Jessica Thompson. Teacher Development Multi-Year Study Series. Evaluation of Australia’s investment in teacher development in Lao PDR: Interim report 1. Australian Council for Educational Research, 2022. http://dx.doi.org/10.37517/978-1-74286-674-1.

Повний текст джерела
Анотація:
The Government of Lao People’s Democratic Republic (Lao PDR) is undertaking significant primary education reforms, supported by the Australian Government's Department of Foreign Affairs and Trade (DFAT) through its flagship Basic Education Quality and Access in Laos program (BEQUAL). The Australian Government has commissioned a study to investigate how the BEQUAL program is making a difference to improving teaching quality and student learning outcomes. This research is part of a multi-year study series undertaken by DFAT's Education Analytics Service to investigate teacher and learning development initiatives in three countries: Lao PDR, Timor-Leste and Vanuatu. In 2019, the new curriculum for Lao language and other subjects was introduced for Grade 1 and is being phased in across all five primary grades. The new curriculum promotes teaching practices that support pedagogies focused on student-centred approaches, active learning, assessment of student learning progress, and a phonics approach to teaching reading. Teachers are being provided with teacher guides and other teaching and learning resources, and receive face-to-face orientation on the new curriculum. In BEQUAL-targeted districts, education support grants are also available to facilitate additional in-service support for teachers and principals. This study has provided the opportunity to investigate teaching quality and student literacy outcomes in Lao PDR over two rounds of data collection, with another planned for October 2022. The Baseline Report captured ‘state of play’ information in 2019 prior to major curriculum changes, as well as the onset of the COVID-19 pandemic. This summary provides an overview of findings and recommendations from the second year (2021) of the study, following two years of BEQUAL support for the implementation of the new Grade 1 Lao language curriculum.
Стилі APA, Harvard, Vancouver, ISO та ін.
Також вас можуть зацікавити розширені добірки на тему "Targetted capture" для конкретних типів джерел:
Статті в журналах
Ми пропонуємо знижки на всі преміум-плани для авторів, чиї праці увійшли до тематичних добірок літератури. Зв'яжіться з нами, щоб отримати унікальний промокод!

До бібліографії