Дисертації з теми "Supernatants"
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Carlisle, Matthew David. "Degradation of human alpha- and beta-defensins by culture supernatants of Porphyromonas gingivalis." Thesis, University of Iowa, 2010. https://ir.uiowa.edu/etd/651.
Повний текст джерелаSané, Sabine [Verfasser], and Roland [Akademischer Betreuer] Zengerle. "Using microorganisms culture supernatants to supply enzymes to biofuel cells and extend cathode lifetime." Freiburg : Universität, 2016. http://d-nb.info/1119452686/34.
Повний текст джерелаCrilly, P. J. "The effect of steroids on the immunoregulatory nature of thymic epithelial cell culture supernatants." Thesis, University of Strathclyde, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.371947.
Повний текст джерелаGunkel, Ann Marilyn. "Evaluation of the Mutagenicity and Toxicity of Monoazo Dyes in Wastewater Effluents and Sludge Supernatants." University of Cincinnati / OhioLINK, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1021908155.
Повний текст джерелаZarnegar, Abdolreza. "Purification and Characterization of an Inhibitor of Thymidine Uptake From Culture Supernatants of Human Tonsil Lymphocytes." Digital Commons @ East Tennessee State University, 1987. https://dc.etsu.edu/etd/2833.
Повний текст джерелаRiffel, Amy Marie. "Osteoblasts aggregates cultivated in a 3-dimensional culture environment rigorously respond to Porphyromonas gingivalis culture supernatants." Thesis, University of Iowa, 2011. https://ir.uiowa.edu/etd/1067.
Повний текст джерелаYu, Haiyue [Verfasser]. "Effect of probiotic supernatants on the metabolic activity and survival of Streptococcus mutans in vitro / Haiyue Yu." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2021. http://d-nb.info/124153893X/34.
Повний текст джерелаMilojkovic, Dragana. "The leukaemic micro environment : The effect of tumour supernatant (TSN)." Thesis, King's College London (University of London), 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.500072.
Повний текст джерелаSzatkowska, Beata. "Performance and control of biofilm systems with partial nitritation and Anammox for supernatant treatment." Doctoral thesis, Stockholm : Mark- och vattenteknik, Kungliga Tekniska högskolan, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4462.
Повний текст джерелаGhosh, Shayok. "Optimization of phosphorus recovery from anaerobic digester supernatant through a struvite crystallization fluidized bed reactor." Thesis, University of British Columbia, 2016. http://hdl.handle.net/2429/60128.
Повний текст джерелаApplied Science, Faculty of
Civil Engineering, Department of
Graduate
McNeil, Heather Joan. "Effects of delivery method on serological responses of bighorn sheep to a multivalent Pasteurella haemolytica supernatant vaccine." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ35913.pdf.
Повний текст джерелаStolarczyk, Elzbieta Ilona. "INSIGHTS INTO EXPRESSION, CELLULAR LOCALIZATION, AND REGULATION OF SUPERNATANT PROTEIN FACTOR, A PUTATIVE REGULATOR OF CHOLESTEROL BIOSYNTHESIS." UKnowledge, 2009. http://uknowledge.uky.edu/gradschool_diss/696.
Повний текст джерелаTrumper, Bronwen Bauer. "TOXIC EFFECTS OF CULTURE SUPERNATANT FLUIDS OF HAEMOPHILUS PLEUROPNEUMONIAE IN VITRO AND IN VIVO (RESPIRATORY, SWINE, PLEUROPNEUMONIA)." Thesis, The University of Arizona, 1985. http://hdl.handle.net/10150/275412.
Повний текст джерелаMokashi, Vishwesh. "SUPERNATANT PROTEIN FACTOR: INSIGHTS INTO ITS REGULATION AND ABILITY TO STIMULATE CHOLESTEROL SYNTHESIS IN VITRO AND IN CELL CULTURE." UKnowledge, 2004. http://uknowledge.uky.edu/gradschool_diss/469.
Повний текст джерелаBecker, Eric. "A combinatorial engineering approach to increase the productivity of CHO cells, and proteomic analysis of cell culture supernatant." [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-38407.
Повний текст джерелаMokashi, Vishwesh. "Supernatant protein factor Insights into its regulation and ability to simulate cholesterol synthesis in vitro and in cell cultural /." Lexington, Ky. : [University of Kentucky Libraries], 2004. http://lib.uky.edu/ETD/ukytoxi2004d00189/Mokashi.pdf.
Повний текст джерелаTitle from document title page (viewed Jan. 6, 2005). Document formatted into pages; contains viii, 88p. : ill. Includes abstract and vita. Includes bibliographical references (p. 80-86).
Smith, Robert C. "Ecological Factors in Design of a Two-Sludge Nitrifying Activated Sludge System Incorporating Side-Stream Treatment of Anaerobic Digester Supernatant." University of Cincinnati / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1291307830.
Повний текст джерелаConnan, Romain. "Feasibility of anammox for the treatment of sewage sludge digester supernatant : from inoculum enrichment and cultivation to process configurations and emissions." Thesis, Rennes 1, 2016. http://www.theses.fr/2016REN1S103/document.
Повний текст джерелаThis work focuses on the study of a wastewater treatment process entitled "anammox". It is a biological process based on the metabolism of a group of bacteria of the same name allowing the purification of nitrogen. This work develops a methodology for their identification, their culture and for the implementation of bioreactor treatment at the laboratory scale
Serra, Ryan J. Nares Salvador. "Phenytoin and its metabolite, 5-p-Hydroxyphenyl-, 5-Phenylhydantoin, decrease supernatant levels of matrix-metalloproteases in the human macrophage implications for drug-induced gingival overgrowth /." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2009. http://dc.lib.unc.edu/u?/etd,2427.
Повний текст джерелаTitle from electronic title page (viewed Sep. 3, 2009). "... in partial fulfillment of the requirements for the degree of Master of Science in the School of Dentistry Periodontology." Discipline: Periodontology; Department/School: Dentistry.
Björkander, Sophia. "Immune maturation and lymphocyte characteristics in relation to early gut bacteria exposure." Doctoral thesis, Stockholms universitet, Institutionen för molekylär biovetenskap, Wenner-Grens institut, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-134054.
Повний текст джерелаAt the time of the doctoral defense, the following paper was unpublished and had a status as follows: Paper 4: Manuscript.
Smedberg, Vilma. "Extraktion av proteiner från olika råvaror för humankonsumtion." Thesis, Högskolan i Borås, Akademin för textil, teknik och ekonomi, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-23318.
Повний текст джерелаAs a result of today's increase in global population and meat production, alternative and more sustainable meat substitutes are highly sought after. As a basic raw material, plants or microbial products can be utilized for protein-rich sustainable food products. This work aims to investigate various protein extraction methods currently used to obtain a protein isolate that can be of use in the food industry. Selected raw materials studied and discussed are pea, quinoa, flour worm and filamentous fungus. General information for each raw material is discussed, however focus has been on studying processes for extracting proteins into an isolate from the raw materials. The work includes general methods to protein extraction as well as more specific methods from individual attempts to extract a specific protein. Finally, the extraction methods are compared, the raw materials that are currently in commercial use are discussed as well as thoughts why some raw materials have not progressed as far in protein extraction as others are compared.
Rovira, Clusellas Meritxell. "Estrategias para la diferenciación in vitro de células ES de ratón a células acinares pancreáticas." Doctoral thesis, Universitat Pompeu Fabra, 2007. http://hdl.handle.net/10803/7104.
Повний текст джерелаLas células madre embrionarias (ES) de ratón han sido utilizadas en la última década para generar in vitro células completamente diferenciadas de varios linajes celulares. No obstante, la capacidad de las células ES a diferenciarse a tipos celulares de origen endodérmico es muy limitada. El objetivo principal de este proyecto ha consistido en desarrollar estrategias para diferenciar células ES de ratón a células pancreáticas acinares con una elevada eficiencia mediante 1) la optimización de las condiciones de cultivo con tal de activar vías de señalización implicadas en el desarrollo/diferenciación pancreáticas; 2) la sobreexpresión de factores transcripcionales maestros utilizando vectores virales con el fin de recapitular específicamente un programa de diferenciación acinar; 3) la selección genética de las células comprometidas al linaje acinar con el objetivo de purificar las células acinares diferenciadas.
Mediante la integración de estos abordajes, hemos conseguido aislar células que comparten características fenotípicas con células acinares inmaduras según la expresión de marcadores de diferenciación y la respuesta funcional a secretagogos.
Exocrine pancreatic diseases such as chronic pancreatitis (PC) or pancreatic cancer are major health issues in Europe. In CP, the acinar tissue is substituted by ductal complexes. In addition, it is difficult to maintain the differentiated phenotype of the acinar cells in culture as within few days an acinar-ductal transdifferentiation takes place.
In the last decade, mouse embryonic stem cells (mES) have been used to generate differentiated cells of a variety of cellular lineages in vitro. However, the ability of ES cells to differentiate into endodermal lineages is limited. The main objective of this project has focused on the development of strategies to differentiate mES to pancreatic acinar cells with high efficiency by means of: 1) Optimization of cell culture conditions to activate signalling pathways involved in pancreatic differentiation/development; 2) the overexpression of master transcription factors involved in pancreas development using viral vectors in order to recapitulate specific acinar differentiation program; 3) the genetic selection of cells committed to the acinar linage in order to purify the differentiated cells.
The integration of these different strategies allowed us to isolate cells that share phenotypic features with immature acinar cells according to the expression of differentiation markers and the functional response to acinar secretegogues.
Peulve, Pascal. "Inhibition de la croissance, in vitro, des cellules hématopoïétiques humaines par les surnageants de cultures de la lignée Raji." Rouen, 1987. http://www.theses.fr/1987ROUES022.
Повний текст джерелаDeblais, Loic. "Understanding of Salmonella-phytopathogen-environment-plant interactions and development of novel antimicrobial to reduce the Salmonella burden in fresh tomato production." The Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1534437638478448.
Повний текст джерелаLedesma, Lina Marcela Sánchez. "Produção de estruvita a partir de esgoto doméstico." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/3/3147/tde-14082015-144656/.
Повний текст джерелаThe shortage of the phosphorus sources and high-energy consumption associated to the nitrogen fertilizers production will be problems in the future. The nutrient recovery from wastewater as struvite has been considered as an alternative to alleviate these problems. In Latin America, production of struvite from wastewater is not yet a wellknown technology and therefore the purpose of this work is to contribute to a better understanding of the phenomena involved. This research work was performed in three phases: 1) production of struvite from upflow anaerobic sludge blanket reactor effluent; 2) production of struvite from anaerobic digester supernatant of enhanced biological phosphorus removal process (ADS-EBPR) and 3) influence of calcium in the struvite produced in the phase 2. In three phases, the magnesium concentrations were adjusted to obtain the preset phosphorus:magnesium (P:Mg) ratios and the pH was adjusted between 8,00 and 10,50. The results of the first phase showed that it is not possible to produce struvite in the upflow anaerobic sludge blanket reactor effluent in the tested conditions. However, removal of nitrogen and phosphorus was observed because amorphous calcium and magnesium phosphates were produced. The results of the second phase showed that it is possible to produce struvite in the ADS-EBPR and the molar consumptions of phosphate (PO43-), ammonia (NH4+) and magnesium (Mg2+) or removals (%) should not be the only parameters to evaluate the struvite formation, because other compounds crystallize or precipitate and reduce the quality of the mineral. In the similar conditions tested in this phase, a P:Mg ratio 1:2 and pH 9,50 assure maximum nutrients recovery as struvite with minimum impurities concentration, facilitating its subsequent use as fertilizer. The results of the third phase showed that amorphous calcium or magnesium phosphates were produced on the struvite surface.
Prisciandaro, Luca David. "Probiotic-derived factors for the treatment and prevention of 5-fluorouracil-induced intestinal mucositis." Thesis, 2013. http://hdl.handle.net/2440/96820.
Повний текст джерелаThesis (Ph.D.) -- University of Adelaide, School of Animal and Veterinary Sciences, 2013
Yi-fang, Chen, and 陳怡芳. "Study on Enzyme Activities of Fermented Supernatants of Two Native Strains Isolated from Konjac Degradation." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/28355142591455270653.
Повний текст джерела大葉大學
生物產業科技學系
93
The two better native strains of dyu-cs-3 and dyu-cs-6, isolated from konjac degradation, were used to preliminarily study their cultural conditions and enzymatic activities of the fermented supernatants for degrading (hydrolyzing) the commercial konjac gel. It was found that the optimal cultural temperature and initial pH of cultural media for the two strains were at 37℃ and 8.0, while the suitable contents of cultural media with 3% konjac gel in 250mL flasks for dyu-cs-3 and dyu-cs-6 were 50mL and 75mL, respectively. For dyu-cs-3 and dyu-cs-6, the highest concentrations (0.15 and 0.16mg/mL, respectively) of reducing sugar were obtained at 48 h of cultivation. After 10 min centrifugation (10000 × g) at 4℃, the enzymatic activity of the fermented supernatants for degrading konjac gel was investigated. The optimal reaction temperature and pH were at 40℃ and 8.0, respectively, while the activity decreased significantly at higher reaction temperature (50℃ or higher) and lower pH (pH 7.0 or lower). The thermal enzyme stability of the dyu-cs-3 supernatants was only at 30℃, while that of the dyu-cs-6 ones was at 30~40℃. The thermal stability of the dyu-cs-3 enzyme seemed be better than that of the dyu-cs-6 one. For the enzymatic pH-stability test at room temperature, the pH-stability range of the dyu-cs-3 and dyu-cs-6 fermented supernatants was at pH 5.0~7.0, and their enzymatic activities retained about 70~80% for first 120 min treatment. The optimal releasing rate of reducing sugar from the substrate-konjac gel was at first 30 min for the dyu-cs-3 and dyu-cs-6 enzymes at pH 8.0 and 40℃. The storage temperatures of the enzymatic activities of dyu-cs-3 and cs-6 fermented supernatants were also investigated. During 7-day storage the dyu-cs-3 enzyme still had about 80-100% of activity at 6℃ and 30℃, while the dyu-cs-6 enzyme had close 100% of activity at 30℃ and had close around 80% at 6℃. For the reaction kinetics of the enzymes of the two fermented supernatants, the reaction constants for both the dyu-cs-3 and dyu-cs-6 enzymes were very similar. The reaction constants, Km and Vmax, for the dyu-cs-3 enzyme were 1.614mg/mL and 0.015mg/mL•min, while the reaction constants, Km and Vmax, for the dyu-cs-6 one were 1.617mg/mL and 0.014mg/mL•min, respectively.
Barra, Vera Diana [Verfasser]. "Regulation of arginase II expression in macrophages by supernatants of apoptotic cells / vorgelegt von Vera Diana Barra." 2010. http://d-nb.info/1011435802/34.
Повний текст джерелаCapela, Emanuel Augusto Vieira. "Innovative and sustainable platforms for the downstream processing of antibody-based biopharmaceuticals." Doctoral thesis, 2022. http://hdl.handle.net/10773/33484.
Повний текст джерелаOs anticorpos, e em particular a imunoglobulina G (IgG), são considerados uma das pontas de lança da indústria biofarmacêutica, apresentando elevada relevância para o tratamento de várias doenças e sendo, por vezes, a única terapia disponível para algumas patologias. Apesar do seu amplo potencial, a extração e purificação destas biomoléculas a partir dos seus meios biológicos complexos com elevada qualidade e pureza é ainda baseada em abordagens com várias etapas e com elevado custo associado. Portanto, o desenvolvimento de processos a jusante alternativos, económicos e eficientes, capazes de fornecer elevadas quantidades de anticorpos terapêuticos a um custo reduzido é altamente necessário. Novas estratégias baseadas em líquidos iónicos (LIs) foram então investigadas nesta tese de Doutoramento para o processamento a jusante de anticorpos. Os LIs foram escolhidos principalmente devido ao seu carácter de solventes customizáveis. Esta característica dos LIs permite adequar a polaridade, interações e seletividade dos processos desenvolvidos, permitindo superar algumas limitações técnicas dos processos convencionais. Três tipos de plataformas baseadas em LIs foram investigadas para o processamento a jusante de anticorpos, nomeadamente sistemas aquosos bifásicos (SAB), partição trifásica e líquidos iónicos suportados. SAB contendo LIs como adjuvantes foram investigados, permitindo a extração e purificação de anticorpos humanos (anticorpos policlonais e monoclonais) com um bom desempenho em uma única etapa. LIs mais biocompatíveis e sustentáveis foram também estudados como compostos formadores de fases de SAB, demonstrando em simultâneo a possibilidade de utilizar abordagens de partição trifásica baseadas em SAB para a purificação e recuperação de anticorpos humanos. Finalmente, foram propostas novas matrizes cromatográficas baseadas em líquidos iónicos suportados, capazes de capturar e/ou purificar anticorpos a partir dos seus meios biológicos complexos através de dois mecanismos distintos, nomeadamente através dos modos negativo e positivo. Em suma, nesta tese de Doutoramento foi demonstrado que os LIs, se adequadamente concebidos, podem ser aplicados com sucesso na extração, purificação e/ou recuperação de anticorpos humanos, sendo plataformas alternativas promissoras para o processamento a jusante de biofármacos baseados em anticorpos.
Programa Doutoral em Engenharia Química
Chiang, Ming-I., and 蔣明怡. "Antimicrobial Activity of Commercial Yogurt Supernatant." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/98825594452998195134.
Повний текст джерела大葉大學
生物產業科技學系碩士在職專班
95
The inhibition effect (antimicrobial activities) of the supernatant of four commercial drinking yogurts, which were hold at 4oC, on the growth of five microbial strains (Streptococcus aureus BCRC10451, Salmonella typhimurium BCRC12947, Bacillus subtilis BCRC11634, E. coli BCRC11634, Pseudomonas aeruginosa BCRC11633) was investigated in the study. The cell count of lactic acid bacteria (LAB), pH value and titrable acidity of the supernatants were also studied. During the 16-day storage time at 4oC, the LAB cell count of all the commercial yogurt supernatant still kept maintaining over 8.9-9.5 log CFU/mL, the pH values of the supernatants little gradually changed from original 4.32-4.21 to 4.03, the titrable acidity was little gradually increased from 0.48%-0.58% to 0.58%-0.63%. For antimicrobial activity, the inhibition zones of the yogurt supernatants for all microbial strains except for P. aeruginosa (no quantitative detection) by using agar diffusion were at the range of 23.0-36.0 mm, especially the range of 34.5-36.0 mm for S. aureus. The antimicrobial activity of the original supernatant with pH 4-5 was better that of the neutral one adjusted to pH 7.0. In addition, the pH value of the agar for microbial diffusion was decreased from 2.58 to 2.22 as increasing the addition concentration of lactic acid (0.3%-2.0%). For the antimicrobial activity of lactic acid, the inhibition zones for the five microbial strains increased with increasing the addition concentration. Based on these results, there was no antimicrobial effect of the original or neutral supernatant (pH 4.0-5.0 or 7.0, respectively) on P. aeruginosa growth. This may be due to that the pH value or the lactic acid content of the supernatant was less than 2.48 or more than 0.3%. However, the supernatants of the four commercial yogurts only showed much effective on the growth inhibition of effects of S. aureus, S. typhimurium, B. subtilis and E. coli.
Huang, Hui. "Pilot scale phosphorus recovery from anaerobic digester supernatant." Thesis, 2003. http://hdl.handle.net/2429/15338.
Повний текст джерелаZimmo, Nouf. "Effect of P. gingivalis supernatant and Kavain on bone biology." Thesis, 2019. https://hdl.handle.net/2144/36569.
Повний текст джерелаBurešová, Nedvídková Jaroslava. "Stanovení cytokinů v supernatantu homogenátu jater u potkanů s jaterní steatózou." Master's thesis, 2010. http://www.nusl.cz/ntk/nusl-282894.
Повний текст джерелаWood, Cody D. "Catalytic Gasification of Pretreated Activated Sludge Supernatant in Near-critical Water." Thesis, 2011. http://hdl.handle.net/1807/31641.
Повний текст джерелаZhang, Ying. "Struvite crystallization from digester supernatant-reducing caustic chemical addition by CO₂ stripping." Thesis, 2006. http://hdl.handle.net/2429/18399.
Повний текст джерелаApplied Science, Faculty of
Civil Engineering, Department of
Graduate
Tsai, Chia-Ling, and 蔡佳玲. "Hydrogen production from thermophilic aerobic digested sludge supernatant by purple nonsulfur bacteria." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/03841758820654246960.
Повний текст джерела國立中興大學
環境工程學系所
95
Thermophilic aerobic digestion (TAD) which applies thermotolerant microbes and their extracellular enzymes to degrade waste activated sludge (WAS) is considerably new and dynamic technique. It was mentioned that when TAD process was modified to be operated under microaerobic condition, the accumulation of volatile fatty acid (VFAs) was expected. Hydrogen production by microbes is a new technology for hydrogen production. One of the most important hydrogen producing bacteria is purple nonsulfur photosynthetic bacteria. VFAs are important substrates for purple nonsulfur bacteria to grow and produce hydrogen. Thus, combining the modified TAD process with photohydrogen production makes sludge removal and energy recycle possible. In order to increase the accumulation concentration of VFAs in TAD reactor, first we raised the initial concentration of the SS. When initial SS concentration of sludge is 13000 mg/L, and the inoculation concentration of Geobacillus thermocatenulatus S2 was 645.4 mg/L, the accumulated concentration of VFAs was 1105 mg/L, which was the highest. The yield and C/N ratio was 0.28 mgVFAs/△mg VSS and 3.1, respectively. Second, the experiments with or without aeration was discussed. The result showed that TAD system with aeration had better reaction rate. Furthermore, the 2 L TAD reactor was operated in a continuous model at 65℃. The result indicated that when HRT is 24 hr, the accumulated concentration of VFAs was 330 mg/L, which was higher than when HRT was 12 hr. The NH4+-N concentration of TAD effluent was too high to inhibiting the hydrogen production of purple nonsulfur bacteria. The pH value of the effluent was adjusted to be alkaline and aerated to remove ammonia. The result showed that when pH value was 12.0, NH4+-N concentration could be removed under detection limitation within 17 hr. However, when NH4+-N concentration of the TAD effluent was higher than 100 mg/L, the efficiency of aeration was low. Moreover, the VFAs concentration of the TAD effluent decreased. Hydrogen production by Rhodopseudomonas palustris WP 3-5 using the pretreated effluent of TAD was investigated. The highest accumulated hydrogen volume was 25.8 ml (while the headspace was 50 ml) when using the TAD effluent which has already removed NH4+-N by aeration. On the other hand, we found that the distillery wastewater contained high concentration of VFAs and low concentration of NH4+-N, so we mixed the distillery wastewater with the effluent of TAD. The result showed that the best ratio of distillery wastewater to the effluent of TAD for H2 production was 2:5, and the highest accumulated H2 volume and hydrogen production rate (HPR) was 263.9 ml and 12.4 ml H2/L-culture/hr, respectively (while the headspace was 150 ml). We also used the dilution distillery wastewater as substrate for hydrogen production. The result indicated that when content of distillery wastewater was 40%, the highest accumulated H2 volume and HPR was 278.3 ml and 13.06 ml H2/L-culture/hr, respectively. Furthermore, comparing the H2 producing efficiency of mixed wastewater and diluted distillery wastewater, it was observed that the diluted distillery had higher HPR, but the mixed wastewater had higher accumulated H2 volume.
Li, Jowitt Z. X. "Recovering biodegradable carbon from a thermophilic aerobic digestion supernatant for biological nutrient removal." Thesis, 2001. http://hdl.handle.net/2429/13754.
Повний текст джерелаTantinirundr, Usakorn. "Phosphorus removal in an aerobic supernatant by struvite crystallization without addition of chemicals." 2000. http://catalog.hathitrust.org/api/volumes/oclc/44638311.html.
Повний текст джерелаTypescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 53-56).
Mazalová, Lenka. "Interakce makrofágů a buněk rakovinné prostatické linie PC-3." Doctoral thesis, 2018. http://www.nusl.cz/ntk/nusl-425302.
Повний текст джерелаYeganegi, Maryam. "The Effect of Lactobacillus rhamnosus GR-1 Supernatant on Cytokine Production and Prostaglandins in Gestational Tissues." Thesis, 2010. http://hdl.handle.net/1807/32035.
Повний текст джерела"Investigations of MicroRNAs in urine supernatant for the diagnosis of bladder cancer and the potential functional roles of miR-99a." 2012. http://library.cuhk.edu.hk/record=b5549530.
Повний текст джерелаUrothelial carcinoma of the bladder (UCB) is the second most common malignancy in the urological system with high recurrence rate. Current gold standard examination for diagnosis is urethrocystoscopy, which is an invasive procedure. Although numerous molecular markers in blood or urine have been proposed as diagnostic biomarkers for bladder cancer, none of them could replace urethrocystoscopy in clinical practice. There are accumulating evidences suggesting microRNA dysregulation might be related to the pathogenesis of UCB. However, the exact functions of these microRNAs in UCB remain unknown. In this thesis, the role of selected microRNAs in urine supernatant was investigated in the diagnosis of UCB and also the carcinogenesis of UCB.
In brief, a high-throughput microarray was carried out on nine supernatants of urine from UCB and normal subjects, and also four pairs of tissue from UCB and normal mucosa. Ten microRNA candidates were then identified. Quantitative RT-PCR was used to validate these microRNAs on a set of 18 pairs of tumor tissue and normal mucosa. Eventually, six potential candidate microRNAs were selected and then validated as diagnostic tools on the samples of urine supernatants from 71 patients (50 of known UCB and 21 of normal subjects). The expression levels of these selected microRNAs were further evaluated in the urine supernatants of 20 patients after tumors resections. MiR-125b and miR-99a were the two most significantly down-regulated microRNAs in the urine supernatants of patients with UCB. Moreover, the degree of down-regulation was associated with the pathological grade of the tumor. A combined index of miR-125b and miR-99a in urine supernatant had a sensitivity of 86.7%, specificity of 81.1%, and a positive predicted value of 91.8% for diagnosing UCB. When used to discriminate high-grade from low-grade UCB, miR-125b alone had a sensitivity of 81.4%, specificity of 87.0% and PPV of 93.4%. After transurethral resections, the expression levels of both microRNAs were significantly increased compared to pre-operative levels.
In further studies on the role of microRNAs on the development of UCB, miR-99a was selected for further studies. The precursor of miR-99a was temporally transfected into 3 bladder cancer cell lines: T24, UMUC3 and J82. The proliferation ability was noticed to be suppressed mildly in UMUC3, but not the other. Meanwhile, migration and invasion abilities were inhibited by miR-99a in the all 3 cell lines. Potential targets of miR-99a were predicted from several prediction databases. Subsequently, in Western Blot study, the protein level of very low density lipoprotein receptor (VLDLR) was showed to be down-regulated by miR-99a. Thereafter, a plasmid constructed with 3’UTR of VLDLR was transfected into cytoplasm, which confirmed VLDLR mRNA was a direct target of miR-99a. All 3 cells lines showed the same effect on suppression of migration and invasion after knockdown of VLDLR. N-cadherin was identified as a down-stream molecule responsible for the migration and invasion suppression in this pathway.
This study confirmed microRNA expression in urine supernatants was a feasible approach for the assessment of biomarkers, and miR-125b and miR-99a showed promising results in the diagnosis and grading of UCB. Furthermore, we showed that miR-99a suppressed tumor migration and invasion by directly targeting VLDLR.
Detailed summary in vernacular field only.
Zhang, Dingzuan.
Thesis (Ph.D.)--Chinese University of Hong Kong, 2012.
Includes bibliographical references (leaves 107-131).
Abstract and appendix also in Chinese.
Abstract --- p.I
摘要 --- p.III
Acknowledgments --- p.V
Abbreviations --- p.VII
List of figures --- p.IX
List of Tables --- p.XI
Content --- p.XII
Chapter Chapter I: --- General Introduction
Chapter 1.1 --- Bladder cancer --- p.1
Chapter 1.1.1 --- The incidence of bladder cancer
Chapter 1.1.2 --- The burden of bladder cancer to the health care system
Chapter 1.1.3 --- Risk factors for bladder cancer
Chapter 1.1.4 --- Pathology grading system in bladder cancer
Chapter 1.1.5 --- Current diagnostic methods and treatment for bladder cancer
Chapter 1.2 --- Biomarkers for bladder cancer --- p.7
Chapter 1.2.1 --- The advantages of biomarkers in blood and urine for the diagnosis of bladder cancer
Chapter 1.2.2 --- Biomarkers in blood for bladder cancer
Chapter 1.2.3 --- Biomarkers in the urine for bladder cancer
Chapter 1.2.4 --- Current concerning problems with biomarkers
Chapter 1.3 --- MicroRNAs and bladder cancer --- p.11
Chapter 1.3.1 --- Post-trancriptional function of microRNAs
Chapter 1.3.2 --- The function of microRNAs in tumor
Chapter 1.3.3 --- Prospects of detecting microRNA in cell-free fluid in tumor
Chapter 1.4 --- MicroRNA target identification --- p.15
Chapter 1.4.1 --- Prediction of microRNA target
Chapter 1.4.2 --- Validation of microRNA target
Chapter 1.4.3 --- Validation of direct interaction between microRNA and target RNA
Chapter 1.4.4 --- Validation of direct binding of microRNA and mRNA in vivo
Chapter 1.5 --- Migration and invasion of bladder cancer --- p.19
Chapter 1.5.1 --- The biological process of migration in bladder cancer
Chapter 1.5.2 --- Epithelial to mesenchymal transition in bladder cancer
Chapter 1.6 --- Objectives of this study --- p.21
Chapter Chapter II --- MicroRNAs in urine supernatant: potential useful markers for bladder cancer screening
Chapter 2.1 --- Introduction --- p.23
Chapter 2.2 --- Materials and methods --- p.26
Chapter 2.2.1 --- Ethics Statement
Chapter 2.2.2 --- Patients and samples
Chapter 2.2.3 --- RNA extraction
Chapter 2.2.4 --- MicroRNA microarray
Chapter 2.2.5 --- Quantitative real-time polymerase chain reaction (RT-PCR)
Chapter 2.2.6 --- Statistical methods
Chapter 2.3 --- Results --- p.31
Chapter 2.3.1 --- MicroRNA screening by microRNA microarray
Chapter 2.3.2 --- Independent validation of the ten selected microRNAs by qRT-PCR on tissue
Chapter 2.3.3 --- Verification of the six validated microRNAs in urine supernatants as tumor markers
Chapter 2.3.4 --- MiR-125b and miR-99a in urine supernatants were useful for the diagnosis of bladder cancer
Chapter 2. --- 3.5 MiR-125b and miR-99a were two highly correlated microRNAs
Chapter 2.3.6 --- Expression levels of miR-125b and miR-99a increased after tumor resection
Chapter 2.4 --- Discussion --- p.47
Chapter Chapter III: --- MiR-99a suppresses migration and invasion in bladder cancer by targeting VLDLR
Chapter 3.1 --- Introduction --- p.53
Chapter 3.2 --- Materials and methods --- p.56
Chapter 3.2.1 --- Human tissue samples and bladder cancer cell lines
Chapter 3.2.2 --- RNA extraction and Polymerase Chain Reaction
Chapter 3.2.3 --- MicroRNA and plasmid transfection
Chapter 3.2.4 --- Western Immunoblotting
Chapter 3.2.5 --- Agarose gel electrophoresis
Chapter 3.2.6 --- Luciferase assay
Chapter 3.2.7 --- MTT proliferation assay
Chapter 3.2.8 --- Apoptosis assay
Chapter 3.2.9 --- Cell cycle analysis
Chapter 3.2.10 --- Cell migration Assay
Chapter 3.1.11 --- Cell invasion assay:
Chapter 3.2.12 --- Statistical methods:
Chapter 3.3 --- Results --- p.67
Chapter 3.3.1 --- MiR-99a was significantly down-regulated in bladder cancer
Chapter 3.3.2 --- Precursor microRNA was successfully transfected into bladder cancer cell lines
Chapter 3.3.3 --- MiR-99a had little effect on cell proliferation
Chapter 3.3.4 --- MiR-99a had little effect on cell apoptosis and cell cycle
Chapter 3.3.5 --- Over-expression of miR-99a suppressed cell migration in bladder cancer
Chapter 3.3.6 --- Over-expression of miR-99a also suppressed invasion ability in bladder cancer
Chapter 3.3.7 --- Target prediction for miR-99a using 8 target prediction databases
Chapter 3.3.8 --- Protein level of VLDLR was down-regulated by miR-99a in bladder cancer
Chapter 3.3.9 --- VLDLR was a direct target of miR-99a
Chapter 3.3.10 --- VLDLR mRNA was not down-regulated correspondingly by miR-99a
Chapter 3.3.11 --- MiR-99a suppressed down-stream protein of VLDLR in Reelin pathway
Chapter 3.3.12 --- Knockdown of VLDLR also suppressed cell migration and invasion
Chapter 3.3.13 --- N-cadherin was the down-stream protein responsible for the suppression of migration and invasion in miR-99a/VLDLR pathway
Chapter 3.4 --- Discussion --- p.93
Chapter Chapter IV: --- Conclusion and prospective --- p.101
Appendix --- p.105
Reference --- p.107
Chen, Wan-Yi, and 陳菀貽. "Degradation of waste activate sludge by thermophilic bacteria and photobiological hydrogen production from digested sludge supernatant by purple nonsulfur bacteria." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/34085521733839651698.
Повний текст джерела國立中興大學
環境工程學系所
94
Thermophilic aerobic digestion (TAD) which applies thermotolerant microbes and their extracellular enzymes to degrade waste activated sludge (WAS) is a considerably new and dynamic technique. It is no doubt that if TAD process is modified to be operated under microaerobic condition, the accumulation of volatile fatty acids (VFAs) is expected. VFAs are suitable substrates for purple nonsulfur bacteria to grow and produce hydrogen. Thus, combining the modified TAD process with photohydrogen production makes sludge removal and energy recycle possible. In order to decide the best operating strategies of the TAD reactor, the factor of inoculation, initial pH, aeration rate and several kinds of sludge would be investigated. The optimum initial pH and inoculation of Geobacillus thermocatenulatus S2 were 6.5 ~ 7.5 and 7.5 %, respectively. Under microaerobic condition (0.25 vvm), 2L paper sludge were digested for 58 h, 65 ℃. The result indicated that the removal efficiencies of SS and VSS were 42.40 and 52.73 %, respectively. The final concentration of VFAs in the liquid phase of the digested sludge were 530 mg/L. It was observed that the liquid phase of sludge was contributed to VFAs. Subsequently, inoculation of Geobacillus stearothermophilus J was discussed, and there was no significant influence. Furthermore, 2 L TAD reactor was operated in a continuous model at 65℃. The result indicated that the higher concentration of VFAs was investigated. The result of hydrogen production by strain Rhodopseudomonas palustris WP3-5 with different dilution ratios of the digested liquid revealed that no hydrogen was produced in all dilution solution ( 0.4X, 0.6X, 0.8X ,non-dilute ). Three kinds of chemicals (Fe, buffer, Fe and buffer) were added respectively, the experiment results showed that, strain WP3-5 could grow in the presence of higher NH4+ concentration, but there was still no hydrogen production. An tempt was made to find the effect of C/N ratio, the experiment results illustrated that, the lower NH4+ concentration, the higher pH value, when C/N was 1:7, production of hydrogen was not improved. Thus, for photohydrogen production by WP3-5, it seems that there was higher VFAs content and lower NH4+ concentration, hydrogen might be produced.
Britton, Ahren Thomas. "Pilot scale struvite recovery trials from a full-scale anaerobic digester supernatant at the City of Penticton Advanced Wastewater Treatment Plant." Thesis, 2002. http://hdl.handle.net/2429/14071.
Повний текст джерелаLiu, Hong-yi, and 劉鴻毅. "Study on the roles and quantification of 3-hydroxy fatty acids in the soma and supernatant of cultured Burkholderia cepacia complex." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/78dh4t.
Повний текст джерела國立高雄師範大學
生物科學研究所
97
Study on the roles and quantification of 3-hydroxy fatty acids in the soma and supernatant of cultured Burkholderia cepacia complex Abstract Although Burkholderia cepacia complex (BCC) divided into 9 of recA genomovars in present, the virulence to hosts in these genomovars were still undifferentiated. We hypothesized that, in cultural supernatants, the amounts of C14:0 3-OH fatty acids, the unique components of BCC lipopolysaccharides (LPS), acted as a biomarker for virulence, were related to the degrees of pathologically inflammatory effects on animals with BCC infection. Thirty-two strains were identified to BCC gonomorvarⅢa by the outcomes of biochemical and molecular tests, and typed to their genetic independence by the profiles of cellular fatty acid and randomly amplified polymorphic DNA (RAPD), respectively. To determine the concentration of fatty acids in supernatants, the quantitative profiles of each fatty acid methyl ester (FAME) analyzed by gas chromatography mass spectrometry (GC/MS) was developed. In this study, the instrument detection limits were reached to 26 ng/ml. The optimal reaction was defined as >90% in esterification for each fatty acid. The processing of samples were performed as that, after a 7 d-incubation, the cultural supernatants were acted as reactants and sequentially reacted to alkaline hydrolysis for 30 min and esterification for 10min. Among of the cultures of these isolates, the concentration of C14:0 3-OH fatty acid in supernatants was ranged from 19.3±0.4 to 133.7±3.6ng/ml. The molar ratio of C14:0/C16:0 3-OH fatty acid was calculated to be 1.78±0.3. With multiple regression analysis, a positive correlation was shown that the concentration of 3-OH fatty acids in supernatants of each isolates was against some levels of pathological effect (area of cell debris in liver) of Balb/c mice with tested BCC infection individually (R2=0.682). Results suggested that the concentration of C14:0 3-OH fatty acid in BCC cultural supernatants was acted as an indicator of virulence to the mice with the bacterial infection for 2 d. Keywords:Burkholderia cepacia complex, lipopolysaccharides, C14:0 3-OH fatty acid, Gas chromatography mass spectrometry
Li, Yu-Ting, and 李侑庭. "Effects of the supernatant from Lactobacillus acidophilus and Lactobacillus plantarum fermented with curcium on upregulating the neprilysin and degrading the Beta-amyloid." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/84174630934644109675.
Повний текст джерела國立中興大學
食品暨應用生物科技學系所
105
Alzheimer''s disease is a type of dementia that causes problems with memory, thinking and behavior. It is a chronic neurological dysfunction that symptoms usually develop slowly and get worse over time. The study showed that Alzheimer''s disease as a kind of neurodegenerative disease. The disease is related to the fibrous β-amyloid peptide (Aβ) accumulated in brain. Neprilysin (NEP) is a kind of enzyme in body which can degrade Aβ and prevent the occurrence of Alzheimer''s disease. However the level of NEP will decrease with age. The study showed that turmeric can increase the level of NEP and slow down the Aβ accumulation rate. Due to the poor bioavailability of turmeric, it is not easy to be absorbed by body. As a result, the physiological effect of turmeric is limited. This study uses turmeric as material for Lactobacillus acidophilus and Lactobacillus plantarum fermentation. Obtain the supernatant after centrifugation and filtration, then use the cell model to explore the samples to know whether it can enhance the level of NEP protein and degrade the Aβ accumulation or not. The result of bacteria screening showed that Lactobacillus acidophilus and Lactobacillus plantarum that fermented in turmeric grew better than other bacteria. Therefore, we treated the SH-SY5Y with fermentation supernatant, then used the Western blot and ELISA to analysis the the level of NEP and the Aβ-degrading activity respectively. The result of NEP level by treated with different concentration fermention was showed that the fermented group is higher than Control and Vehicle. Furthermore, the L. acidophilus 10% is the highest than others. Analysis the Aβ-degrading activity of SH-SY5Y treated with L. acidophilus 100%, L. acidophilus 10%, L. plantarum 10%, L. plantarum 100%, Vehicle, Control, we obtain the Aβ residue was 21830 pg/ml, 4194 pg/ml, 24436 pg/ml, 4648 pg/ml, 38497 pg/ml, 27966.7 pg/ml respectively. The results as above were far lower than Original group (172903 pg/ml). It also indicated that the LAB fermentation groups were lower than Control and Vehicle. Furthermore, the L. acidophilus 10% was the lowest. It showed L. acidophilus 10% had the best Aβ-degrading activity, as the same as the trend of NEP level result. These results indicate the supernatant from Lactobacillus acidophilus and Lactobacillus plantarum that fermented with Curcuma longa can effectively enhance the Neprilysin protein expression and degrade the β-amyloid.
Becker, Eric [Verfasser]. "A combinatorial engineering approach to increase the productivity of CHO cells, and proteomic analysis of cell culture supernatant / vorgelegt von Eric Becker." 2009. http://d-nb.info/992696410/34.
Повний текст джерелаWang, Tong, and 王彤. "Effects of speciation of polyaluminum chloride (PACl) on the removal of particles and speciation of residual Al in the supernatant after sedimentation." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/58454146062765818582.
Повний текст джерела國立交通大學
環境工程系所
104
Polyaluminum chloride (PACl) is one of most frequently used coagulant for particle destabilization in water treatment. The speciation of PACl coagulant strongly affects the coagulation performance and residual Al species in the supernatant. The aim of this study was to investigate the effects of hydrolyzed Al species on the removal of particles and speciation of residual Al after sedimentation. Three kinds of PACl coagulants with different fraction of monomeric Al (Ala), polymeric Al (Alb) and colloidal Al (Alc), namely PACl-1 (Ala/Alb/Alc=14/64/22), PACl-2 (Ala/Alb/Alc=47/31/22) and PACl-3 (Ala/Alb/Alc=56/22/22), were used in jar testing to evaluate the performance of coagulation with and without FeCl3 dosing. For coagulation with dual dosing, the ratio between PACl and FeCl3 dosing is 2:1. The composition and speciation of residual Al in the supernatant after sedimentation for coagulation with various PACl coagulants were determined with and without dual dosing. The result has shown that PACl coagulant with high ratio of Alb/Ala possesses strong charge neutralization ability to effectively destabilize particles at the optimum dosage of 2 mg/L as Al, which lowers total residual Al as well as dissolved Al in the supernatant, even though dual coagulation (PACl dosing mixed with FeCl3 coagulant). At such conditions, the average size and fractal dimension of flocs formed by mixing with PACl coagulants with high ratio of Alb/Ala are much smaller compared to PACl coagulants with lower ratio. The dissolved residual Al in the supernatant increases with Ala/Alb ratio of PACl coagulants regardless of dosage. With increasing PACl dosage, the quantity of Ala decreases and Alc increases in the supernatant after PACl-2 and PACl-3 coagulation, while Ala increases and Alc decreases after PACl-1 coagulation. The quantity of Alb in the supernatant increases with Alb/Ala of PACl coagulant.
Czubak-Prowizor, Kamila. "Mechanizmy niepożądanych reakcji związanych z przetaczaniem koncentratów krwinek czerwonych w badaniach in vitro oraz in vivo." Phd diss., 2020. http://hdl.handle.net/11089/32966.
Повний текст джерелаBoulanger, Mary Louise. "The effect of varying air supply upon supernatant quality in autoheated thermophilic aerobic digesters treating waste sludge from a biological phosphorus removal process." Thesis, 1994. http://hdl.handle.net/2429/3602.
Повний текст джерелаChiang, Shu-Ching, and 江淑靜. "Effects of Probiotcis-Fermented Supernatant from Substrates Containing Oligosaccharides on the Inhibition of Colon Cancer Cell Proliferation and Antitoxicity of Human Colon Cell Line Int-407." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/51619154504009541155.
Повний текст джерела臺灣大學
食品科技研究所
98
Prebiotics are usually non-digestible oligosaccharides to human. They are resistant to gastric acid and digestive enzymes so that they could reach into the large intestines and were fermented by the intestinal microbes. Recently, some research has focused on the functions of the metabolites and short chain fatty acids that they might suppress the proliferation of colon adenocarcinoma cells. The aim of this study was to evaluate the anticytotoxic and antigentoxic effects of fermented supernatant by Lactobacillus casei 01, Bifidobaterium lactis Bb-12 and Lactobacillus plantarum in inulin, fructooligosacchardie (FOS), isomaltooligosaccharide (IMO) and xylooligosaccharide (XOS) contained medium. The growth curves showed that three strains of probiotics could grow in the oligosaccharides-contained medium and the pH values were decreasing during the fermentation. The results showed that the fermented supernatant could suppress the proliferation of HT-29 (p < 0.05) except L. plantarum-IMO group. In addition, the XOS-fermented supernatant showed the best suppression. The viability of 4NQO group was 54% and almost of cell viability in the test groups increased by treating with the fermented supernatant (p < 0.05), and the FOS-fermented supernatant showed the best ability in the promoting the cell viability after 4NQO induced cell damages. Furthermore, we observed that the DNA tails reduced in the test groups significantly by comet assay (p < 0.05). From the analysis of organic acids, the XOS-fermented supernatant contained higher concentration of total SCFAs that might contribute to the decreasing viabilities on HT-29. FOS-fermented supernatant contained the highest butyrate concentration which might contribute to the anticytotoxicity and antigenotoxicity.