Готові списки джерел за темами / Supernatants

Добірка наукової літератури з теми "Supernatants"

Автор: Grafiati
Опубліковано: 10 грудня 2022
Оновлено: 28 січня 2023

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Статті в журналах з теми "Supernatants"

1

Tamaru, Yutaka, Sadaharu Ui, Koichiro Murashima, Akihiko Kosugi, Helen Chan, Roy H. Doi, and Bo Liu. "Formation of Protoplasts from Cultured Tobacco Cells and Arabidopsis thaliana by the Action of Cellulosomes and Pectate Lyase from Clostridium cellulovorans." Applied and Environmental Microbiology 68, no. 5 (May 2002): 2614–18. http://dx.doi.org/10.1128/aem.68.5.2614-2618.2002.

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ABSTRACT The crude culture supernatants from Clostridium cellulovorans were tested for their ability to convert plant cells to protoplasts. The supernatants readily released protoplasts from cultured tobacco cells and Arabidopsis thaliana. The crude culture supernatant from pectin-grown cells was more active than supernatants from glucose-, cellobiose-, xylan-, and locust bean gum-grown cells. After removal of cellulosomes, the crude culture supernatant lost its protoplast formation activity. The protoplast formation activity of the crude culture supernatant from C. cellulovorans was more effective than those of commercial enzymes based on protein content.
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2

Zavizion, Boris, A. John Bramley, and Ioannis Politis. "Effects ofStaphylococcus aureusproducts on growth and function of bovine mammary myoepithelial cellsin vitro." Journal of Dairy Research 62, no. 4 (November 1995): 577–86. http://dx.doi.org/10.1017/s0022029900031307.

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SUMMARYThe effects of culture supernatants conditioned by the growth ofStaphylococcus aureusM60 onin vitrogrowth and functional properties of bovine mammary myoepithelial cells were examined. Myoepithelial cell proliferation was reduced byStaph, aurexisM60 culture supernatants. Exposure of myoepithelial cells to culture supernatants of isogeneic mutants ofStaph, aureusM60 that produced either α or β toxins reduced proliferation, but to a lesser extent than supernatants from the wild type strain. Thus, α and β toxins may play some role in affecting myoepithelial cell proliferation. Of the cells tested, 42% contracted following addition of oxytocin (10–7M) in the culture medium. Treatment of myoepithelial cells for 15 min withStaph, aureusM60 supernatants, prior to addition of oxytocin in the culture medium, increased the number of cells that contracted to 92%. Exposure of cells for 3 h to the same supernatant, prior to addition of oxytocin in the culture medium, abolished oxytocin responsiveness, had no effect on immunolocalization of actin and vimentin, but affected the localization of α-actinin within myoepithelial cells. Treatment of myoepithelial cells for 3 h with a combination of purified staphylococcal proteinases XVI and XVII-B abolished oxytocin responsiveness and mimicked the effect of theStaph, aureusculture supernatant. We conclude thatStaph, aureusM60 culture supernatant affected proliferation and functional properties of myoepithelial cells.
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3

Stachowiak, R., M. Lyzniak, B. K. Budziszewska, K. Roeske, J. Bielecki, G. Hoser, and J. Kawiak. "Cytotoxicity of Bacterial Metabolic Products, including Listeriolysin O, on Leukocyte Targets." Journal of Biomedicine and Biotechnology 2012 (2012): 1–9. http://dx.doi.org/10.1155/2012/954375.

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Bacterial toxins can exhibit anticancer activities. Here we investigated the anticancer effects of the listeriolysin O toxin produced byListeria monocytogenes. We found that supernatants ofListeria monocytogenesstrains (wild type, 1189, and 1190) were cytotoxic to the Jurkat cell line and human peripheral blood mononuclear cells (PBMC) in a concentration-dependent manner. The supernatant of strain 1044, not producing listeriolysin O, was inactive. The supernatants ofListeriastrains were also cytotoxic toward B cells of chronic leukemia patients, with no significant differences in activities between strains. We also tested supernatants ofBacillus subtilisstrains BR1-90, BR1-S, and BR1-89 producing listeriolysin O. BR1-S and BR1-89 were cytotoxic to PBMC and to Jurkat cells, the latter being more sensitive to the supernatants. BR1-90 was not hemolytic or cytotoxic to PBMC, but was cytotoxic to Jurkat cells in the concentration range of 10–30%, suggesting that listeriolysin O is selectively effective against T cells. Overall, the response of human peripheral blood mononuclear and human leukemia cell lines to bacteria supernatants containing listeriolysin O depended on the bacteria strain, target cell type, and supernatant concentration.
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4

Yu, F., Y. Itoyama, J. Kira, K. Fujihara, T. Kobayashi, T. Kitamoto, A. Suzumura, N. Yamamoto, Y. Nakajima, and I. Goto. "TNF-beta produced by human T lymphotropic virus type I-infected cells influences the proliferation of human endothelial cells and fibroblasts." Journal of Immunology 152, no. 12 (June 15, 1994): 5930–38. http://dx.doi.org/10.4049/jimmunol.152.12.5930.

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Abstract Human T lymphotropic virus type I (HTLV-I) is linked to adult T cell leukemia as well as to HTLV-I-associated myelopathy/tropical spastic paraparesis. In this report, we studied the effects of HTLV-I-infected cell supernatants on HUVEC, fibroblasts, and glioma cells. The HTLV-I-infected cell supernatants (HUT102 and MT-2) strongly inhibited the proliferation of HUVEC, although they enhanced the proliferation of the fibroblasts. Regarding the glioma cells, only the MT-2 supernatant showed weak inhibitory effects on the proliferation. However, the HTLV-I-uninfected cell supernatants showed no effects on these target cells. The biologic activities of both HUT102 and MT-2 supernatants were found to be dose dependent and were reduced by heat treatment at 100 degrees C for 5 min, but not at 56 degrees C for 30 min. These activities were not dependent on the concentrations of HTLV-I viral particles and were only minimally affected by the presence of anti-HTLV-I Abs. A bioassay of various cytokines revealed that the activity of TNF was much higher in the HUT102 and MT-2 supernatants than in the HTLV-I-uninfected cell supernatants (MOLT-4, Jurkat, and K-562). rTNF-alpha and rTNF-beta also showed strong inhibitory effects on HUVEC as well as on the enhancement of the fibroblast growth. With the use of Sephadex G-100 column chromatography, we obtained the highest activities from the 60- through 70-kDa fractions of the HUT102 supernatant and some activities from the 20- through 30-kDa fractions. The biologic activities of both the whole HUT102 supernatant and its active fractions were completely blocked by anti-TNF-beta mAb, although they were not blocked by anti-TNF-alpha mAb. In a Western blot assay, the 25- and 27-kDa bands of TNF-beta were shown clearly in the HUT102 supernatant, although no TNF-alpha bands appeared. These findings suggest that TNF-beta is present in either its oligomeric or monomeric form in the HTLV-I-infected cell supernatants and is also mainly responsible for the supernatants' effects on HUVECs and fibroblasts.
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5

Berger, M., EM Wetzler, and RS Wallis. "Tumor necrosis factor is the major monocyte product that increases complement receptor expression on mature human neutrophils." Blood 71, no. 1 (January 1, 1988): 151–58. http://dx.doi.org/10.1182/blood.v71.1.151.151.

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Abstract Interleukin-1 (IL-1) and other monocyte products have several important effects on the systemic response to infection in addition to their roles in lymphocyte stimulation. The present studies were carried out to determine whether products of stimulated monocytes activated circulating neutrophils (PMN) to increase expression of receptors for C3b (CR1) and C3bi (CR3), which are necessary for optimal margination, migration, and phagocytosis. Supernatants of human mononuclear cells that had been stimulated with lipopolysaccharide (LPS) or purified protein derivative (PPD) contained both tumor necrosis factor (TNF) and IL-1 and increased CR1 and CR3 expression on isolated PMNs. Supernatants of unstimulated cultures, media alone, or LPS or PPD alone had little or no effect. Supernatant effects were detectable at 1:3,000 final dilution and appeared to have a characteristic slow time course. These supernatants also caused dose- and time-dependent secretion of PMN granular constituents, but maximal receptor expression was accompanied by secretion of less than 10% of the cells' content of lysozyme and less than 16% of the B12 binding protein. Immunoadsorption studies showed that the supernatant's activity could be removed by anti- TNF but not by anti-IL-1. Recombinant IL-1 had no effect on receptor expression, but recombinant TNF increased CR1 and CR3 expression with kinetics similar to the supernatants. These results thus indicate that TNF is the major monocyte product that increases CR1 and CR3 expression on mature blood neutrophils. This would result in increased margination and phagocytic activity and may be an important systemic effect that would help the host eradicate infection.
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6

Berger, M., EM Wetzler, and RS Wallis. "Tumor necrosis factor is the major monocyte product that increases complement receptor expression on mature human neutrophils." Blood 71, no. 1 (January 1, 1988): 151–58. http://dx.doi.org/10.1182/blood.v71.1.151.bloodjournal711151.

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Анотація:
Interleukin-1 (IL-1) and other monocyte products have several important effects on the systemic response to infection in addition to their roles in lymphocyte stimulation. The present studies were carried out to determine whether products of stimulated monocytes activated circulating neutrophils (PMN) to increase expression of receptors for C3b (CR1) and C3bi (CR3), which are necessary for optimal margination, migration, and phagocytosis. Supernatants of human mononuclear cells that had been stimulated with lipopolysaccharide (LPS) or purified protein derivative (PPD) contained both tumor necrosis factor (TNF) and IL-1 and increased CR1 and CR3 expression on isolated PMNs. Supernatants of unstimulated cultures, media alone, or LPS or PPD alone had little or no effect. Supernatant effects were detectable at 1:3,000 final dilution and appeared to have a characteristic slow time course. These supernatants also caused dose- and time-dependent secretion of PMN granular constituents, but maximal receptor expression was accompanied by secretion of less than 10% of the cells' content of lysozyme and less than 16% of the B12 binding protein. Immunoadsorption studies showed that the supernatant's activity could be removed by anti- TNF but not by anti-IL-1. Recombinant IL-1 had no effect on receptor expression, but recombinant TNF increased CR1 and CR3 expression with kinetics similar to the supernatants. These results thus indicate that TNF is the major monocyte product that increases CR1 and CR3 expression on mature blood neutrophils. This would result in increased margination and phagocytic activity and may be an important systemic effect that would help the host eradicate infection.
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7

Jiménez-Vargas, N. N., M. Guzman Rodriguez, Y. Yu, E. Neary, A. E. Lomax, D. E. Reed, and S. Vanner. "A242 STOOL FROM IBS-D PATIENT WITH A HISTORY OF A DYSBIOTIC AND NON-DYSBIOTIC ONSET MODULATE NEURONAL EXCITABILITY VIA DIFFERENT MECHANISMS." Journal of the Canadian Association of Gastroenterology 5, Supplement_1 (February 21, 2022): 134–35. http://dx.doi.org/10.1093/jcag/gwab049.241.

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Abstract Background We have shown that Irritable Bowel Syndrome diarrhea-predominant (IBS-D) patients with a history of a dysbiotic-like onset have distinct stool metabolomic profiles versus those with a non-dysbiotic-like onset. IBS stool supernatants can sensitize mouse colonic afferent nerves via both histamine and proteases. However, it is unknown if stool supernatants from the two IBS-D subgroups modulate the excitability of nociceptive neurons via different neuroactive mediators Aims To evaluate whether there are differences in neuroactive mediators within stool supernatants from subgroups of IBS-D patients that can modulate the excitability of nociceptive neurons. Methods Stool samples from healthy control (HC) (N=5) and IBS-D patients with a dysbiotic (N=7) or non-dysbiotic-like (N=7) onset, was homogenized with Krebs solution and filtered. Proteolytic activity was assessed using casein as a substrate with and without protease inhibitors. Histamine was quantified by ELISA. DRG neurons from C57BL/6 mice were incubated overnight or acutely (30 min) with stool supernatants. Some neurons were pre-incubated with PAR2 (GB83, 10 μM) or H1R (pyrilamine, 1μM) antagonists prior to supernatant incubation. Changes in neuronal excitability were assessed with perforated patch-clamp by measuring the rheobase (current that elicits an action potential). Results Proteolytic activity in dysbiotic-like (57.9 U/μg, p<0.05) but not non-dysbiotic like (37.4 U/μg) stool supernatant was increased compared to HC (25.2 U/μg). Serine inhibitor decreased proteolytic activity of dysbiotic (46.6%, p<0.05) and non-dysbiotic (34.2%, p<0.01) supernatant whereas cysteine, aspartic and metalloproteases inhibitors had no effect. Histamine was increased 78% (p<0.05) in IBS-D compared to HC. No differences in proteolytic activity and histamine concentration between IBS-D subtypes were found. In patch-clamp recordings, overnight incubation with dysbiotic (19%, p<0.05) and non-dysbiotic (22%, p< 0.01) stool supernatant decreased rheobase of DRG neurons compared to HC. No difference in rheobase was observed between IBS-D subtypes. GB83 blocked the overnight actions of supernatants from both subgroups, but pyrilamine had no effect. In contrast, following acute incubation, pyrilamine blocked the hyperexcitability evoked by dysbiotic like supernatant (60pA vs 48pA, p<0.001) but had no effect on non-dysbiotic like supernatants. Conclusions Proteases in stool supernatants from IBS-D patients increase neuronal excitability but only those with a history of dysbiotic like onset acutely increase neuronal excitability through H1 receptors. These findings suggest that stool supernatants from subgroups of IBS-D may modulate nociceptor excitability via different mechanisms. Funding Agencies CIHRAmerican Neurogastroenterology and Motility Society
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8

Kanof, M. E., W. Strober, W. C. Kwan, N. A. O'Connell, and S. P. James. "CD4+ Leu-8+ T cell supernatant activity that inhibits Ig production." Journal of Immunology 147, no. 1 (July 1, 1991): 155–61. http://dx.doi.org/10.4049/jimmunol.147.1.155.

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Abstract The subpopulation of CD4+ T cells that expresses the Leu-8 peripheral lymph node homing receptor suppresses PWM-stimulated Ig synthesis. To determine the mechanism of this suppression, the immunoregulatory activity of culture supernatants obtained from peripheral blood CD4+ Leu-8+ T cells cultured with anti-CD3 mAb and PMA (Leu-8+ supernatant) was determined. Leu-8+ supernatant suppressed PWM-stimulated Ig synthesis in cultures containing non-T cells and CD4+ Leu-8- T cells. In contrast, the supernatant from CD4+ Leu-8- T cells did not suppress Ig synthesis. The inhibitory activity of CD4+ Leu-8+ T cell supernatants could not be accounted for by a deficiency or excess of IL-2, IL-4, IFN-gamma, IL-6, or PGE2. In studies examining the effect of CD4+ Leu-8+ supernatant on T cells, the supernatant did not alter either mitogen-induced proliferation or the helper function of CD4+ Leu-8- T cells. In studies examining the effect of CD4+ Leu-8+ supernatant on B cells, the supernatant inhibited Staphylococcus aureus Cowan I strain-induced B cell Ig secretion but not B cell proliferation. The suppressor activity of Leu-8+ supernatant was eliminated by protease treatment and was eluted by HPLC in two main peaks, with molecular sizes of 44 and 12 kDa. In summary, these studies indicate that supernatants from activated CD4+ Leu-8+ T cells directly suppress B cell Ig production.
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Wang, Yuehong, Tao Wang, Shanshan Xiao, Ruifang Mao, and Rui Lin. "Identify driver mutants in lung cancer by supernatants of pleural effusion and monitor resistance to targeted therapy." Journal of Clinical Oncology 37, no. 15_suppl (May 20, 2019): e14554-e14554. http://dx.doi.org/10.1200/jco.2019.37.15_suppl.e14554.

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e14554 Background: Malignant pleural effusion (MPE) from lung cancer is an attractive alternative for molecular testing due to advantages of non-invasiveness and less heterogeneity. Previously, cell pellet blocks of MPE are widely used. This study is to further address whether supernatants of MPE is a suitable source to identify key oncogenic mutants in lung cancer and provide more information for clinical molecular testing. Methods: MPE samples from 12 lung cancer patients were centrifuged to obtain supernatants and cell pellets, and DNA were extracted. The DNA samples were analyzed by a targeted next generation sequencing (NGS) panel using Illumina HiSeq platform. Results: In a pilot study, paired cancer tissue and MPE samples were obtained from 3 lung cancer patients and analyzed using a comprehensive 500-gene cancer panel. Nine mutants were identified both in the tissue samples and MPE samples. We then analyzed another 9 lung cancer patient samples to detect oncogenic mutants using an 18-gene panel. In total, 8 mutants including EGFR L858R, exon 19 deletion, or T790M mutants were identified in MPE samples. For the total 17 mutants from the 12 MPE samples, 10 mutants were observed in both matched supernatant and cell pellet of MPE, of which more pairs (6 out of 10) had supernatants with higher abundance of mutants than cell pellets. More importantly, 7 of the 17 mutants were only detected in the supernatants of the matched MPE. Taken together, these results suggest that supernatant of MPE is a more suitable source to detect key driver mutants of lung cancer. Interestingly, 2 patients had both tyrosine kinase inhibitor (TKI) resistant mutant T790M and TKI sensitive mutants detected in supernatants of MPE; both patients had prior treatment of EGFR TKI, consistent with the development of TKI resistant mutant and supporting the utility of supernatants of MPE in monitoring TKI resistance. Conclusions: This study suggest supernatant of MPE is a more suitable source for identifying key driver mutants for lung cancer and can also be used to monitor response to targeted therapy. The study provides evidence of using supernatant of MPE as alternative source for molecular testing and thus direct precise targeted therapy and effect surveillance.
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Neary, E., N. N. Jiménez-Vargas, A. E. Lomax, D. E. Reed, and S. Vanner. "A243 HISTAMINE H1-RECEPTOR ANTAGONISTS SUPPRESS THE HYPEREXCITABILITY OF NOCICEPTIVE NEURONS EXPOSED TO IBS-C PATIENT STOOL SUPERNATANTS." Journal of the Canadian Association of Gastroenterology 5, Supplement_1 (February 21, 2022): 135–36. http://dx.doi.org/10.1093/jcag/gwab049.242.

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Abstract Background Abdominal pain is the primary cause of morbidity in many chronic GI disorders such as IBS, but the molecular mechanisms contributing to pain signaling are unclear. Stool supernatants from patients with diarrhea-predominant IBS increase the excitability of DRG neurons compared to supernatants from healthy controls, suggesting that mediators in the stool may sensitize nociceptors. Additionally, histamine has been implicated as a mediator of hypersensitivity in IBS patients. However, it is unclear if stool supernatants from constipation-predominant IBS (IBS-C) patients affect the excitability of DRG neurons. Furthermore, it is unknown if specific neuro-mediators in stool such as histamine impact the excitability of nociceptive neurons in this subgroup of IBS patients. Aims To evaluate whether IBS-C stool supernatants induce nociceptive signaling in DRG neurons compared to healthy control (HC) stool supernatants. If so, to evaluate the role of histamine 1 (H1) receptors in DRG neuron nociceptive signaling initiated by mediators in IBS-C stool supernatants. Methods IBS-C (n=5) and HC (n=2) patient stool was collected, filtered, and dissolved with Krebs solution in a 1:8 (g/v) dilution. Dorsal root ganglion (DRG) neurons from C57BL/6 mice were incubated with HC or IBS-C stool supernatant for 30 minutes. To evaluate whether histamine in stool supernatants can sensitize H1 receptors on nociceptors, DRG neurons were pre-incubated with the H1-receptor antagonist pyrilamine (1μM, 30 min) before stool supernatants. Changes in DRG neuronal excitability were recorded using perforated patch-clamp techniques to measure the rheobase (minimum input current needed to elicit an action potential) and the resting membrane potential (RMP). Results In neurons incubated with IBS-C stool supernatants (n=28) the rheobase decreased (63%) compared to healthy controls (78 ± 13.7 pA; n=6). This effect was reversed in DRG neurons pre-incubated with the H1 receptor antagonist, pyrilamine, (n=26) (77 ± 5.9 pA, p<0.001) compared to neurons incubated with IBS-C supernatant alone (50 ± 5.13 pA; n=28). No changes were found in the RMP. The data were analyzed with the non-parametric Kruskal-Wallis test. Conclusions IBS-C stool supernatants increase the excitability of DRG neurons compared to HC. Furthermore, the H1-receptor antagonist pyrilamine inhibits the neuronal hyperexcitability evoked by mediators in IBS-C patient stool. These findings suggest that the neuroactive metabolite histamine may contribute to visceral pain experienced by patients with IBS-C. Further studies are needed to examine whether similar signaling to nociceptive neurons by stool supernatants occurs in other subtypes of IBS. Funding Agencies CAG, CIHR
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Більше джерел

Дисертації з теми "Supernatants"

1

Carlisle, Matthew David. "Degradation of human alpha- and beta-defensins by culture supernatants of Porphyromonas gingivalis." Thesis, University of Iowa, 2010. https://ir.uiowa.edu/etd/651.

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Porphyromonas gingivalis produces proteases capable of degrading cytokines, host heme proteins, and some antimicrobial peptides. In this work, I show that P. gingivalis culture supernatants fully or partially degrade human neutrophil peptide alpha-defensins and human beta-defensins after 30 minutes. This observation suggests that proteases from P. gingivalis degrade defensins and this activity could abrogate defensin-related innate immune functions.
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2

Sané, Sabine [Verfasser], and Roland [Akademischer Betreuer] Zengerle. "Using microorganisms culture supernatants to supply enzymes to biofuel cells and extend cathode lifetime." Freiburg : Universität, 2016. http://d-nb.info/1119452686/34.

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3

Crilly, P. J. "The effect of steroids on the immunoregulatory nature of thymic epithelial cell culture supernatants." Thesis, University of Strathclyde, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.371947.

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4

Gunkel, Ann Marilyn. "Evaluation of the Mutagenicity and Toxicity of Monoazo Dyes in Wastewater Effluents and Sludge Supernatants." University of Cincinnati / OhioLINK, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1021908155.

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5

Zarnegar, Abdolreza. "Purification and Characterization of an Inhibitor of Thymidine Uptake From Culture Supernatants of Human Tonsil Lymphocytes." Digital Commons @ East Tennessee State University, 1987. https://dc.etsu.edu/etd/2833.

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Lymphocytes from human tonsils were cultured in the absence of serum for 3 days. In the presence of the concentrated culture supernatant the proliferative response of PBL, to con A, as measured by the uptake of ('3)H-tdr, was significantly reduced. The suppressor substance was referred to as SMAL (suppressor of mitogen activated lymphocytes). The estimated molecular weight of SMAL under nondenaturing conditions was 100,000-300,000. SMAL also suppressed the incorporation of ('3)H-tdr by a variety of mouse and human tumor cell lines. The activity of SMAL was sensitive to pronase and heating at 100(DEGREES)C for 30 minutes but insensitive to RNase. Treatment with DNase, however, enhanced the activity of SMAL. SMAL activity was also destroyed by treatment with 5% TCA, 0.4 M HCl or 60% acetonitrile, but resistant to 6 M urea or dialysis against pH 2 buffer for 24 hours. SMAL activity was precipitated in 40-80% ammonium sulfate saturation. When applied to a phenyl-sepharose column no activity was recovered. SMAL was not produced by heat-killed tonsil lymphocytes or lymphocytes-treated with cycloheximide. Maximal production occurred in the first 24 hours of culture, and progressively less was produced in subsequent 24-hour intervals. Both T- and B lymphocyte-enriched culture supernatants contained SMAL. SMAL adhered strongly to DEAE-cellulose, but less than two-fold purification was achieved. Using QMA-Accell anion exchange medium, a 5-fold purification of SMAL with higher specific activity was obtained with HPLC. Activity of SMAL was recovered after native polyacrylamide gel electrophoresis by electroblotting to DEAE-cellulose paper followed by eluting the bound materials with salt. Two active components, one corresponding to a large and/or less negatively charged molecule and another corresponding to a small and/or highly acidic molecule, were recovered. HPLC-purified SMAL at relatively low doses inhibited the uptake and phosphorylation of ('3)H-tdr, without significant effect on cell proliferation. The inhibition of ('3)H-tdr uptake was favored over that of ('3)H-udr or ('3)H-adr, and this effect was reversible. At relatively high doses of HPLC-purified SMAL, the growth of mouse thymoma EL-4 and human T cell leukemia CEM-CM(,3) cell lines was inhibited.
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6

Riffel, Amy Marie. "Osteoblasts aggregates cultivated in a 3-dimensional culture environment rigorously respond to Porphyromonas gingivalis culture supernatants." Thesis, University of Iowa, 2011. https://ir.uiowa.edu/etd/1067.

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An exciting alternative to the current methods for bone regeneration is osseous tissue engineering. One such method focuses on enhancement of osteoblast differentiation through rotary cell culture techniques. The response of osteoblast aggregates to periodontal microorganisms and their by-products will ultimately be important in their success as a method of bone regeneration. In this study, I hypothesize that human embryonic palatal mesenchymal (HEPM, ATCC 1486) pre-osteoblast cells produce different cytokine responses depending upon whether they are grown in a 2-dimensional tissue culture flask or a 3-dimensional tissue culture vessel and whether they are exposed to an un-inoculated, sterile Porphyromonas gingivalis growth medium or exposed to a 24 hour, sterile, P. gingivalis culture supernatant. Objectives: My objectives were first to determine and compare the cytokine response of HEPM, ATCC 1486 pre-osteoblast cells depending upon whether they are grown in a 2-dimensional tissue culture flask or a 3-dimensional tissue culture vessel and whether they are exposed to an un-inoculated, sterile P. gingivalis growth medium or exposed to a 24 hour, sterile, P. gingivalis culture supernatant. Methods: In 3 experiments, 5 X 106 HEPM, ATCC 1486 cells were grown in a 2-dimensional tissue culture flask or a 3-dimensional tissue culture vessel and exposed to an un-inoculated, sterile P. gingivalis growth medium or exposed to a 24 hour, sterile, P. gingivalis culture supernatant and incubated for 72 hours in 5% CO2 at 37oC. Media was removed from the tissue culture flasks or rotary vessels at 0, 1, 2, 4, 8, 12, 24, 36, 48, 60, and 72 hours to determine cytokine concentrations in the Luminex 100 IS Instrument (Luminex®, Austin, TX). HEPM, ATCC 1486 pre-osteoblast cell morphology was assessed by light and scanning electron microscopy at 96 hours. Results: In experiment 1, there were increases in IL-6 and IL-8. The IL-6 response of cells grown in a 2-dimensional tissue culture flask was higher than that of cells grown in a 3-dimensional tissue culture vessel. The IL-8 responses of the cells grown in 2-dimensional, 3-dimensional tissue culture were nearly identical. In light and scanning electron microscopy cells appeared normal and HEPM, ATCC 1486 pre-osteoblast cell aggregates were similar to that previously reported. In experiment 2, there were also increases in IL-6 and IL-8. The IL-6 and IL-8 responses of HEPM, ATCC 1486 pre-osteoblast cells grown in a 3-dimensional tissue culture vessel exposed to a 24-hour, sterile, P. gingivalis culture supernatant were higher than cells exposed to un-inoculated, sterile P. gingivalis growth media. In experiment 3, HEPM, ATCC 1486 pre-osteoblast cells grown in 2-dimensional tissue culture flasks and 3-dimensional tissue culture vessel exposed to a 24 hour, sterile, P. gingivalis culture supernatant produced high levels of IL-6, IL-8, and VEGF. Again, in light and scanning electron microscopy, cells appeared normal. Conclusion: HEPM, ATCC 1486 pre-osteoblast cells display different cytokine profiles depending upon the type of vessel they are cultured in. They also rigorously respond to P. gingivalis culture supernatants suggesting that they may respond to the presence of microorganisms commonly found in the oral cavity and play an active role in immunity during their integration following bone regeneration.
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Yu, Haiyue [Verfasser]. "Effect of probiotic supernatants on the metabolic activity and survival of Streptococcus mutans in vitro / Haiyue Yu." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2021. http://d-nb.info/124153893X/34.

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Milojkovic, Dragana. "The leukaemic micro environment : The effect of tumour supernatant (TSN)." Thesis, King's College London (University of London), 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.500072.

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9

Szatkowska, Beata. "Performance and control of biofilm systems with partial nitritation and Anammox for supernatant treatment." Doctoral thesis, Stockholm : Mark- och vattenteknik, Kungliga Tekniska högskolan, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4462.

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10

Ghosh, Shayok. "Optimization of phosphorus recovery from anaerobic digester supernatant through a struvite crystallization fluidized bed reactor." Thesis, University of British Columbia, 2016. http://hdl.handle.net/2429/60128.

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Phosphorus is an essential element for all living organisms, but its supply is limited. On the other hand, phosphorus recovery from domestic wastewater can satisfy 15-20 % of current phosphate rock demand. Moreover, struvite scaling is a concern for wastewater engineers as it clogs various equipment. Recovering phosphorus as struvite pellets from wastewater can yield sustainable solution for both problems. Although several technologies have already been available to recover phosphorus from wastewater with reasonable P- recovery efficiency, these technologies possess a number of shortcomings such as higher capital and operating cost, production of fines instead of pellets etc. This study aimed at optimization of phosphorus recovery from wastewater by developing a sustainable and efficient technology. To accomplish this purpose, a new crystallization fluidized bed reactor (FBR) was developed and impact of different physio-chemicals (supersaturation ratio) and hydrodynamic (up-flow velocity, nozzle velocity and configurations) parameters on its performance were analyzed to determine optimum operating conditions. This reactor achieved over 90% of P removal from synthetic supernatant with up to 18% of P recovery. Lower P-recovery was resulted due to lack of proper harvesting mechanism. Results showed that P-removal efficiency was increased with increase in initial supersaturation ratio up to a value of 6.5. But increase in supersaturation ratio yielded lower P-recovery with higher fines production. A value in the range of 5.5-6.0 was suggested by this study for optimum output. Low up-flow velocity was found to be associated with higher P-removal and recovery efficiency, where high up-flow velocity was found to be associated with the production of more large sized pellets and fines. But, higher nozzle velocity was found to be responsible for accomplishing higher P-removal and recovery efficiency. Two nozzles on opposite side yielded higher P-recovery efficiency with more large sized pellets and lower fine production. Based on these results, this study concluded that 40 cm/min up-flow velocity with 18.04 cm/min nozzle velocity and two nozzles on opposite side might be optimum operating conditions. Analysis on the performance of up-scaled reactor showed that optimum conditions for pilot scale and up-scaled reactor might be different due to different hydrodynamic conditions.
Applied Science, Faculty of
Civil Engineering, Department of
Graduate
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Книги з теми "Supernatants"

1

Hernandez, Marta. The study of ligand binding specificities of the lipid binding proteins: Recombinant human a-tocopherol transport protein (a-ttp), supernatant protein factor (spf) and S. cerevisiae Sec 14p for vitamin e (rrr-a-tocopherol) and other hydrophobic ligands. St. Catharines, Ont: Brock University, Dept. of Biotechnology, 2003.

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2

Immunofluorescence localization of dissociation supernatant and extracellular matrix components in lytechinus pictus sectioned embryos. Northridge, Calif: California State University, 1988.

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Sternbach, Marion. Apheresis in the ICU. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780199600830.003.0268.

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This chapter describes therapeutic plasma exchange, as well as cytapheresis for hyperleukocytosis and essential thrombocythemia, as well as harvesting haematological stem cells (HSC) for transplantation. Instrumentation and techniques are mostly density centrifugation, much less column adsorption for antibodies or membrane filtration for noxious molecules. Pathophysiology of apheresis is dealt with in great detail with emphasis on prevention and treatment of side effects, much more critical in the intensive care unit (ICU) setting. Main manifestations are: hypocalcaemia due to chelation by anticoagulants, hypo- and less hypervolaemia, allergic reactions to sedimenting and volume replacement starches or plasma, depletion of coagulation factors, vitamin K, immunoglobulins, lymphocytes with long lifespan and platelets. Wash-out of drugs for comorbid or underlying conditions occurs inadvertently. Main indications for plasma exchange are thrombotic thrombocytopenic purpura (TTP)/haemolytic uraemic syndrome (HUS) with plasma or cryo-poor supernatant (based on RCT), hyperviscosity syndromes, post-transfusion purpura (PTP) and auto-immune haemolytic anaemia (AIHA), where all other treatments have failed. In cold agglutinin disease, cryoglobulinaemia, coagulation factor inhibitors and ABO incompatible HSC transplants, plasmapheresis has proven useful. Myeloma with renal failure does not seem to benefit significantly from plasma exchange (randomized controlled trials proven).
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Частини книг з теми "Supernatants"

1

Page, Mark, and Robin Thorpe. "Screening Hybridoma Culture Supernatants Using ELISA." In Springer Protocols Handbooks, 779–80. Totowa, NJ: Humana Press, 1996. http://dx.doi.org/10.1007/978-1-60327-259-9_142.

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Page, Mark, and Robin Thorpe. "Screening Hybridoma Culture Supernatants Using ELISA." In Springer Protocols Handbooks, 1947–48. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-198-7_205.

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Page, Mark, and Robin Thorpe. "Screening Hybridoma Culture Supernatants Using Solid-Phase Radiobinding Assay." In Springer Protocols Handbooks, 777–78. Totowa, NJ: Humana Press, 1996. http://dx.doi.org/10.1007/978-1-60327-259-9_141.

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4

Page, Mark, and Robin Thorpe. "Screening Hybridoma Culture Supernatants Using Solid-Phase Radiobinding Assay." In Springer Protocols Handbooks, 1945–46. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-198-7_204.

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Wurm, David Johannes, and Oliver Spadiut. "Purification of Recombinant Glycoproteins from Pichia pastoris Culture Supernatants." In Methods in Molecular Biology, 343–50. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9024-5_17.

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6

Lind, Waldemar, Mona Lietz, Volker Jäger, and Roland Wagner. "Proteolytic Activities in Serum-Free Supernatants of Mammalian Cell Lines." In Animal Cell Culture and Production of Biologicals, 319–27. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-011-3550-4_37.

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7

Dobrica, Mihaela-Olivia, Catalin Lazar, and Norica Branza-Nichita. "Production of Chimeric Hepatitis B Virus Surface Antigens in Mammalian Cells." In Vaccine Delivery Technology, 83–94. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0795-4_7.

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Abstract The small (S) envelope protein of the Hepatitis B Virus (HBV), HBV-S, has the unique ability to self-assemble into highly immunogenic subviral particles (SVPs), in the absence of other viral factors, in eukaryotic cells, including those of nonhepatic origin. This feature is currently exploited for generation of SVPs exposing heterologous epitopes on their surface that can be used as vaccine candidates to target various diseases. Here, we describe a simple and robust method for production of such chimeric HBV-S protein-based SVPs in transiently transfected HEK293T cells and purification from cell supernatants by ultracentrifugation on sucrose cushion and sucrose step gradients. The SVPs obtained by this methodology have been successfully used in immunogenicity studies in animal models.
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8

Tans, C., S. Wattiaux De Coninck, M. M. Gonze, and L. Fabry. "Evaluation of the Proteolytic Activity Present in Cho Cell Culture Supernatants." In Animal Cell Technology, 295–300. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5404-8_46.

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9

Osborne, Matthew, Daniel Bracewell, Jonathan Dempsey, Ray Field, Brendan Fish, and Christy Ritchie. "Rapid Estimation of Human Monoclonal Antibody (IgG4) Concentration in Cell Culture Supernatants." In Animal Cell Technology: From Target to Market, 463–65. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-010-0369-8_112.

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10

Schulz, C., J. H. Vogel, and K. Scharfenberg. "Influence of Pluronic F-68 on the Ultrafiltration of Cell Culture Supernatants." In Animal Cell Technology, 373–78. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5404-8_59.

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Тези доповідей конференцій з теми "Supernatants"

1

Piovella, F., R. Lombardi, M. Vigotti, A. B. Federici, P. M. Mannucci, and E. Ascari. "VON WILLEBRAND FACTOR MULTIMERS IN CULTURED HUMAN ENDOTHELIAL CELLS: COMPARISON BETWEEN CELLULAR STORAGE POOL AND SUPERNATANT." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644100.

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The multimeric composition of von Willebrand factor (vWf) from cultured human endothelial cells (h.e.c.) has been compared with the multimeric composition of vWf from h.e.c. culture supernatant, human normal platelets and plasma. H.e.c. were derived from umbilical cord veins by collagenase digestion, seeded in culture flasks and grown to confluence in TC 199 culture medium, supplemented with 20% foetal calf serum (FCS). At confluency, cells were harvested by rubber policeman, resuspended in 500 |o.l HBSS with protease inhibitors and stored with culture media until assay. H.e.c. and platelet lysates were obtained by freezing-and-thawing (5 times) and by the addition of 1% Sodium Dodecyl Sulphate (SDS) before centrifugation at 10,000 x g for 20 min at 20°C. Plasma, culture supernatants and cell lysates supernatants were run under the same conditions in an SDS 1.4%, LGT-agarose electrophoresis system using a discontinous buffer. Multimeric patterns were shown by radiolabelled affinity purified rabbit anti-human polyclonal antibody. In all the experiments cultured h.e.c. supernatants exhibited all the set of multimers of vWf usually observed in normal plasma. When vWf from cultured h.e.c. extracts was analyzed, a set of multimers with higher molecular weight was shown, with a pattern very similar to platelet's. We conclude that vWf stored in h.e.c. compartments is characterized by higher molecular weight multimers than culture supernatants. This behaviour recalls the differences recorded between plasma and platelet vWf.
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2

Krishnamurti, C., B. M. Alving, and D. G. Wright. "INDUCTION OF PLASMINOGEN ACTIVATOR AND PLASMINOGEN ACTIVATOR INHIBITOR ACTIVITY DURING DIFFERENTIATION OF HL-60 CELLS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644390.

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While increased production of plasminogen activator(PA) has been associated with malignant growth and differentiation, the effect of cell maturation on plasminogen activator inhibitor (PAI) production has not yet been defined. We therefore determined the effect of phorbol myristate acetate(PMA), an agent that induces monocytoid cellular maturation, on the secretion of both PA and PAI in HL-60 cells, a promyelocytic leukemia cell line. Cells were seeded at 5x105/ml and grown in a 5% CO2 incubator at 37°C in a serum-free medium in the absence or presence of 1.6 nM PMA. Experiments were performed either in polypropylene tubes to which cells did not adhere, or in petri dishes that provided surfaces for cellular adherence. Supernatants were assayed for PA activity with a functional chromogenic assay using plasminogen and S-2251 with urokinase (UK) as a standard. PAI was measured by determining the inhibition of exogenous UK (0.05 U/ml final) after incubation with the supernatants at 22°C for 30 min. In the absence of PMA, supernatants of control cells grown in tubes or petri dishes had a PA activity of 2 mU/106 cells at 72 hr. After exposure to PMA for 72 hr, PA activity in supernatants of non-adherent cells grown in tubes increased 50-fold over control, while only an 8-fold increase was measured in supernatants of cells that had been incubated in petri dishes and allowed to undergo adherence. The PA had a Mr of 56 kD as determined by fibrin zymography, and functional activity was completely inhibited by antibodies to UK. While there was no inhibition of exogenous UK in supernatants of control cells grown in tubes or petri dishes, there was a 50% inhibition of UK added to the supernatants of the PMA-treated cells grown under non-adherent conditions in tubes. Inhibition of exogenous UK was 94% by supernatants from PMA-treated cells that had undergone adherence in petri dishes. The inhibitory activity was completely abolished by incubation of the supernatants with IgG purified from antiserum to PAI-2 (courtesy Dr. Kruithof). These data indicate that PMA induces the production of UK-like PA and also an inhibitor similar to PAI-2, which has been found in monocytes. Furthermore, the regulation of both PA and PAI production appears to depend on conditions that affect cellular adherence.
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3

Goodwin, C. A., M. F. Scully, V. Ellis, and V. V. Kakkar. "THROMBIN BINDING FRAGMENT E GENERATED DURING FIBRINOGENOLYSIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642937.

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Binding of fibrinogen by thrombin was measured by inhibition of amidolysis of S2238 and found to be 12 μM. Upon digestion of fibrinogen with plasmin (0.16ugs/mg fibrinogen) for 4 hours at 37°C, thrombin binding activity remained in the supernatants upon heat treatment. The thrombin binding activity in the dialyzed supernatant reached a maximum after two hours coinciding with maximal release of B 1-42 and 45-39 KDa chain fragments. Measured immunologically, levels of fragment E at this time were 45% of the maximum generated after 4 hours digestion. FPA levels in the dialyzed supernatant (measured by RIA and HPLC) after thrombin treatment, were zero and did not increase until 1½ hours after the beginning of digestion, reaching a maximum at 4 hours. The thrombin binding activity generated was stable to further plasmin action. Upon gel chromatography of 2 and 4 hour supernatants, thrombin binding activity coincided closely with fragment E, measured immunologically. Further purification showed the fragment to have Ki for thrombin amidolytic activity of 0.5μM. The fragment also inhibited the thrombin clotting time of plasma but did not affect fibrin monomer polymerization.The fragment was susceptible to very slow inactivation by thrombin but not arvin (though it did inhibit arvin amidolytic activity). A thrombin binding (thrombin inhibitory) fragment is therefore generated during the early stages of f ibrinogenolysis and may be the result of protection by 45 and 39 KDa A α carboxy terminus fragments since E fragments generated in later stages (in the presence of 29 and 25 KDa fragments) do not have this property. These findings may give interesting new insight into thrombin/fibrinogen interaction.
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4

Krauss-Etschmann, Susanne, Inge Kepert, Kerstin Hochwind, Anton Hartmann, Juliano Fonseca, and Philippe Schmitt-Kopplin. "Detection Of Th2 Counteracting Activity In Supernatants From Probiotic Bacteria." In American Thoracic Society 2011 International Conference, May 13-18, 2011 • Denver Colorado. American Thoracic Society, 2011. http://dx.doi.org/10.1164/ajrccm-conference.2011.183.1_meetingabstracts.a2832.

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5

Reyes, Niradiz, Oscar Correa, Alfonso Bettin, Raj K. Tiwari, and Jan Geliebter. "Abstract 4882: Expression profiles of proinflammatory chemokines in prostate cancer cell supernatants." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-4882.

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6

Schaub, R. G., C. J. Dunn, D. E. Tracey, W. E. Fleming, and M. D. Burdick. "THROMBOTIC AND INFLAMMATORY CHANGES IN ENDOTHELIAL CELLS INCUBATED WITH LEUKOCYTES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642860.

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Adhesion of leukocytes (WBC's) to vascular endothelial cells (EC's) is a component of inflammation, thrombosis and atherosclerosis. The purpose of this study was to assess the effect of WBC adhesion on the EC contribution to these pathologic events. Human WBC's were isolated and co-incubated with cultured human umbilical cord EC's. Supernatants and cell lysates (4 wells × 2) were obtained at 0.5,1,2, and 4 hours of incubation. EC's and WBC's (5 × 105) were-incubated alone or in combination. Supernatants and cell lysates were assayed for leukotriene B4 (LTB4), the thromboxane metabolite thromboxane B2 (TXB2) and the prostacyclin metabolite 6-keto prostaglandin FT alpha (6 keto) by RIA. Cell lysates were analyzed for cell associated procoagulant activity (PCA) by an APTT procedure, for plasminogen activator inhibitor (PAI) by an amidolytic assay and for IL-1 by a T-cell co-stimulator assay. Cellular and supernatant LTB4 was unmeasurable for both WBC's and WBC/EC cultures. WBC TXB2 showed a time dependent elevation which was unaffected by EC's. IL-1 activity was measurable at 2 hours and reach 14 U/ml in WBC's and 6 U/ml in EC/WBC cultures. Co-incubation of WBC's with EC's induced a 200% increase in both supernatant and cell associated 6 keto concentrations compared to EC's incubated alone. EC's and WBC's produced no PCA when incubated alone. PCA activity of the EC/WBC co-cultures was measurable at 2 hours and was 300-1500 U/ml after 4 hours. Coincubated EC's had a 50% decrease in cell PAI, suggesting an increased release of inhibitor from the cells. The prostacyclin and PAI release -along with the delayed expression of PCA activity are responses similar to those expected after EC exposure to cytokines. A source of these cytokines appears to be the WBC's which secreted measurable amounts of IL-1. WBC released IL-1 was sufficient to induce biochemical changes in EC's which can stimulate coagulation (PCA synthesis), inhibit fibrinolysis (PAI release), and enhance inflammation (prostacyclin synthesis). These results suggest that the release of WBC IL-1 can be sufficient to produce pro-thrombotic and inflammatory changes in EC's which are similar to those observed with the addition of exogenous IL-1 to EC cultures.
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7

Auad, Ligia Isoni, Roseane Batitucci Passos de Oliveira, Elisabeth Neumann, Afonso de Liguori Oliveira, and Luiza de Alcântara Moraes. "Probiotic Cell-Free Culture Supernatants From Kefir Grains and Inhibition of Listeria Monocytogenes." In XII Latin American Congress on Food Microbiology and Hygiene. São Paulo: Editora Edgard Blücher, 2014. http://dx.doi.org/10.5151/foodsci-microal-310.

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8

Mazur, Witold, Tuula Toljamo, Pentti Nieminen, Katri Vuopala, Steffen Ohlmeier, Hideo Kobayashi, and Vuokko L. Kinnula. "Elevation Of Pulmonary Surfactant Protein A In Sputum Supernatants Of Current Cigarette Smokers." In American Thoracic Society 2011 International Conference, May 13-18, 2011 • Denver Colorado. American Thoracic Society, 2011. http://dx.doi.org/10.1164/ajrccm-conference.2011.183.1_meetingabstracts.a3007.

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9

Minno, G. Di, A. M. Cerbone, F. Cirillo, M. Colucci, N. Semararo, G. Di Santo, P. L. Mattioli, M. Mancini, and A. Quattrone. "ABNORMAL FIBRINOGEN (FIBRINOGEN NAPLES) CHARACTERIZED BY DETECTIVE INTERACTION WITH THROMBIN AND PLASMIN IN TWO YOUNG SIBLINGS WITH ARTERIAL THROMBOSIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644698.

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Prolonged thrombin time (partially corrected by calcium chloride) and normal reptilase time were found in the plasma of two siblings with arterial thrombosis. Their purified fibrinogen showed similar abnormalities as well as impaired fibrino-peptide release in response to thrombin, delayed polymerization of pre-formed fibrin monomers and normal sialic content. Binding of radiolabelled thrombin by patient's fibrin was 30% of normal. Supernatants from patients' fibrin clots contained abnormal amounts of thrombin (not adsorbed by fibrin) and caused abnormal enhancement of platelet aggregation and ATP secretion from platelets exposed to sub-threshold concentrations of ADP or epinephrine. Hirudin suppressed the enhancing effect of the supernatant and substitution of γ-thrombin for α-thrombin led to normalization of platelet response. Studies on fibrinolysis showed that the abnormal fibrinogen from these patients as well as its naturally occurring derivative fibrin are highly resistant to lysis by plasmin. Thus our data support the concept that, in addition to the enhanced activation of platelets by residual free thrombin, thrombosis in these patients is the result of an impaired sensitivity of fibrinogen the lytic effect of plasmin.
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10

Mahmoud, M., F. Hammerschmidt, H. Scheuerlein, and P. J. Gaffney. "IMMUNOASSAYS FOR SINGLE CHAIN URINARY-TYPE PLASMINOGEN ACTIVATOR (SCUPA) IN PLASMA AND IN CELL CULTURE SUPERNATANTS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643604.

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Monoclonal antibodies (mabs) to SCUPA have been generated in balb/C mice by conventional means and have been demonstrated to have no crossreactivity with two chain urinary-type plasminogen activator (TCUPA). These mabs have been used to develop two types of specific assay for SCUPA. Mabs coated on polyvinyl plates in conjunction with polyclonal antibodies (pabs) to TCUPA have allowed the development of a catcher-tag ELISA using alkaline phosphatase-label led goat anti-rabbit IgG as a final step. The sensitivity range of the assay was 0.5 - 10.0 iu/ml. A second bioimmunoassay (BIA) using SCUPA mabs as catcher and inplate development with glu-plasminogen and S-2251 has yielded an assay with a sensitivity range of 0.5 - 10.0 iu/ml. The international unitage ascribed in these assays was derived by comparing the hydrolysis of S-2444 by the I.S. for TCUPA with the purified SCUPA following full activation with plasmin.Using these assays it was found that normal pooled plasmas contained about 1.0 iu of SCUPA antigen which was fully inhibited such that no activity was evident by the BIA assay for SCUPA. This suggests that urokinase in plasma is present in two forms: SCTJPA bound to inhibitor and TCUPA which is biologically active when assayed using a BIA based on immobilised pabs to TCUPA. Cell supernatants from cultured human lung fibroblasts yield a SCTJPA/TCUPA ratio of 70/30 using S-2444 chromogenic assay following a plasmin-mediated SCTJPA-TCUPA conversion step. It was also shown that, in these cell supernatants, SCTJPA was secreted with no inhibitor bound to it, since the BIA and ELISA data were quite similar. A curious feature of these assays, which is as yet unexplained, is the observation that urokinase (both plasmin activated SCTJPA and TCUPA), when immunologically adsorbed on to the PVC-immobilised specific mabs or pabs used in this study, readily activated plasminogen but showed no hydrolytic activity on the chromogenic substrate, S-2444.
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Звіти організацій з теми "Supernatants"

1

Chaiko, D. J., C. J. Mertz, and Y. Vojta. Extraction of long-lived radionuclides from caustic Hanford tank waste supernatants. Office of Scientific and Technical Information (OSTI), July 1995. http://dx.doi.org/10.2172/137312.

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2

Rapko, Brian M. Protocol for Identifying the Presence of and Understanding the Nature of Soluble, Non-pertechnetate Technetium in Hanford Tank Supernatants. Office of Scientific and Technical Information (OSTI), February 2014. http://dx.doi.org/10.2172/1126339.

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3

Serne, R. J., J. M. Zachara, and D. S. Burke. Chemical information on tank supernatants, Cs adsorption from tank liquids onto Hanford sediments, and field observations of Cs migration from past tank leaks. Office of Scientific and Technical Information (OSTI), January 1998. http://dx.doi.org/10.2172/576087.

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4

DE Kurath, DL Blanchard, and JR Bontha. Ion exchange distribution coefficients for {sup 137}Cs and {sup 99}Tc removal from Hanford tank supernatants AW-101 (Envelope A) and AN-107 (Envelope C). Office of Scientific and Technical Information (OSTI), March 2000. http://dx.doi.org/10.2172/752570.

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5

Hobbs, D. T. Supernatant liquid sampling in waste tanks. Office of Scientific and Technical Information (OSTI), September 1992. http://dx.doi.org/10.2172/6875753.

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6

Ploetz, D. K., and I. M. Leonard. Supernatant treatment system design through testing. Office of Scientific and Technical Information (OSTI), December 1988. http://dx.doi.org/10.2172/5859878.

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7

Hobbs, D. T. Supernatant liquid sampling in waste tanks. Office of Scientific and Technical Information (OSTI), September 1992. http://dx.doi.org/10.2172/10125275.

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Geeting, J. G. H., A. M. Rovira, J. R. Allred, R. W. Shimskey, C. A. Burns, and R. A. Peterson. Filtration of Hanford Tank AP-107 Supernatant. Office of Scientific and Technical Information (OSTI), July 2018. http://dx.doi.org/10.2172/1468983.

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9

Geeting, John GH, Jarrod R. Allred, Amy M. Rovira, Rick W. Shimskey, Carolyn AM Burns, and Reid A. Peterson. Crossflow Filtration of Hanford Tank AP-105 Supernatant. Office of Scientific and Technical Information (OSTI), February 2018. http://dx.doi.org/10.2172/1487204.

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10

Hendrickson, D. W. ,. Westinghouse Hanford. Hanford tank waste supernatant cesium removal test plan. Office of Scientific and Technical Information (OSTI), May 1996. http://dx.doi.org/10.2172/657844.

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