Статті в журналах з теми "STATIC ATC DETERMINATION"

The ATC determination method in this study uses line thermal constraints by calculating reactive power flow, and using Linear Power Transfer Distribution Factor. The ATC determination considers the magnitude of the voltage magnitude and the static voltage stability of each bus after the power transfer simulation is performed. Static voltage stability is calculated using the L Index. The results showed that the transfer of power equal to the ATC value in an electric power system caused a decrease in voltage stability or an increase in the L index value on the receiving bus on average 19 times the initial loading condition, even though it was still at a stable level. While the receiver bus voltage magnitude has decreased

The Applied Technology Council (ATC), with funding from the California Seismic Safety Commission developed the document, Seismic Evaluation and Retrofit of Concrete Buildings, commonly referred to as ATC 40. This two-volume, 612-page report provides a recommended procedure for the seismic evaluation and retrofit of concrete buildings. Although the focus is specifically on concrete buildings, the document provides information on emerging techniques applicable to most building types. This paper provides an introduction and overview of the document. The conceptual basis of the procedures is performance-based design using nonlinear static structural analysis. The ATC 40 document comprises a practical guide to the entire evaluation and retrofit process. Topics include performance objectives, seismic hazard, determination of deficiencies, retrofit strategies, quality assurance procedures, nonlinear static analysis using the capacity spectrum method, modeling recommendations, foundation effects, and response limits.

The article presents universal mathematical static current-voltage characteristics- based models of electric arc and indicates primary issues concerned with arc diagnostics related to the determination of the above-named models. Particular attention was paid to the experimental determination of AC-powered arc voltage. Diagnostics-related purposes involved the use of bridge systems with one or two sources of current. Computer-aided simulations involving sinusoidal and trapezoidal current-based excitations made it possible to demonstrate the possibility of obtaining minimum values of bridge unbalance voltage. Such a condition indicated the proper determination of AC-powered arc ignition voltage.

In the conditions of TIG welding, the magnitude of the current is set, but not the voltage of the arc. To determine the arc voltage at a set current, arc length, type of electrode, sharpening angle and others, it is necessary to know the static characteristics of the welding arc. This dependence facilitates the use of simulation modelling methods of welding processes. In the proposed study, the static characteristics of the welding arc in TIG process with 4 types of electrodes were determined experimentally. The magnitude of the current was set in the range of 20 to 200A and the arc voltage was measured. All parameters are measured remotely by a real-time welding monitoring system and transferred to a remote computer via the Internet. Graphs were built based on the recordings, and the numerical results were processed and regression relationships were obtained.

ABSTRACTInvasive pulmonary aspergillosis (IPA) is a devastating disease of immunocompromised patients. Pharmacodynamic (PD) examination of antifungal drug therapy in IPA is one strategy that may improve outcomes. The current study explored the PD target of posaconazole in an immunocompromised murine model of IPA against 10A. fumigatusisolates, including 4Cyp51wild-type isolates and 6 isolates carryingCyp51mutations conferring azole resistance. The posaconazole MIC range was 0.25 to 8 mg/liter. Following infection, mice were given 0.156 to 160 mg/kg of body weight of oral posaconazole daily for 7 days. Efficacy was assessed by quantitative PCR (qPCR) of lung homogenate and survival. At the start of therapy, mice had 5.59 ± 0.19 log10Aspergillusconidial equivalents (CE)/ml of lung homogenate, which increased to 7.11 ± 0.29 log10CE/ml of lung homogenate in untreated animals. The infection was uniformly lethal prior to the study endpoint in control mice. A Hill-type dose response function was used to model the relationship between posaconazole free drug area under the concentration-time curve (AUC)/MIC and qPCR lung burden. The static dose range was 1.09 to 51.9 mg/kg/24 h. The free drug AUC/MIC PD target was 1.09 ± 0.63 for the group of strains. The 1-log kill free drug AUC/MIC was 2.07 ± 1.02. The PD target was not significantly different for the wild-type and mutant organism groups. Mortality mirrored qPCR results, with the greatest improvement in survival noted at the same dosing regimens that produced static or cidal activity. Consideration of human pharmacokinetic data and the current static dose PD target would predict a clinical MIC threshold of 0.25 to 0.5 mg/liter.

This research developed and optimized the methods for simultaneous determination of tetracycline (TCs), macrolide (MLs) and sulfonamide (SAs) antibiotics in soils using accelerated solvent extraction (ASE)- solid-phase extraction (SPE)-high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Methanol-citric acid (pH=4.7) was used as extraction solvent, diatomite (washed by EDTA) was used as dispersing agent. Firstly, soil was extracted by ASE with the parameter conditions: pressure 1500 psi, temperature 70°C, static 10 min, 1 circle, then pre-concentration by SPE and followed by HPLC-MS/MS analysis. Recovery was 86.3%~97.4% for SAs, 67.3%~87.4% for TCs and 68.4%~78.3% for MLs. RSD < 9 % and r > 0.99. Limits of detection (LOD) was 0.5~0.9 ug/kg for SAs, 0.2~1.1 ug/kg for TCs and 0.2~0.3 ug/kg for MLs. This method determined 9 kinds of antibiotics within 15 min, the determination accuracy can meet the requirements of actual analysis.

Object The purpose of this study was to determine the effect of static and dynamic collimator optimization when using a micromultileaf collimator (mMLC) in dynamic-arc stereotactic radiosurgery (SRS) by evaluating the dose to healthy peritumoral tissue. Methods Thirty patients previously treated for intracranial lesions with the BrainLAB mMLC underwent retrospective replanning. Three collimator optimization strategies were compared for a simulated SRS treatment plan, as follows: Strategy 1, static collimation fixed at 90° throughout arcs; Strategy 2, static collimator settings optimized for each arc; and Strategy 3, dynamic collimator settings optimized every 10° throughout treatment arcs. Dose–volume histograms for a 0.7-cm shell of healthy peritumoral tissue were quantitatively compared. Collimator optimization schemes (Strategies 2 and 3) significantly decreased the volume of peritumoral tissue that is irradiated when compared with static collimation at 90° (Strategy 1). The volume was reduced by 40.6% for Strategy 2 (95% confidence interval [CI] ± 11) and by 47.1% for Strategy 3 (95% CI ± 8.1) at the 95% isodose; by 28.4% for Strategy 2 (95% CI ± 4.9) and 39.1% for Strategy 3 (95% CI ± 6) at the 90% isodose; and by 18.2% for Strategy 2 (95% CI ± 8.1) and 25.4% for Strategy 3 (95% CI ± 7.1) at the 80% isodose. Serial collimator optimization throughout the treatment arcs (Strategy 3) reduced the mean volume of peritumoral tissue irradiated when compared with static collimator optimization (Strategy 2), by 16.1% (95% CI ± 1.5) at 95% isodose, by 11.7% (95% CI ± 1) at 90% isodose, and by 8.2% (95% CI ± 1.2) at 80% isodose regions. In specific cases, linear or polynomial functions were formulated to optimize collimator settings dynamically throughout treatment arcs. Conclusions Dynamic collimator optimization during arc-based SRS decreases the volume of healthy peritumoral tissue treated with high doses of radiation and appears to be an effective method of improving target conformality. This study is the first step toward determination of a smoothing function algorithm to allow for true dynamic collimation during SRS.

Autoclaved aerated concrete (AAC) and its hygric parameters are a highly important issue in the field of building physics. There are several methods currently available to determine the equilibrium moisture content of building materials. Beside the conventional ones, new methods are constantly being introduced. This study explores the sorption/desorption properties of of three types of commercially produced AACs with three different bulk densities and demonstrates the application of the relevant methods available to characterize these parameters. The reliable characterization of the studied material was done through the conventional static approach, using the desiccator and an environmental chamber, and a new automated method of dynamic vapor sorption is implemented. The goal is to compare and identify the reliability of all methods used with respect to the efficiency of the data measurement process. Sound consistency between the results of the conventional methods and the experimental data obtained indicates the dynamic vapor sorption technique is highly reliable when measuring the equilibrium moisture content—particularly exemplified during the AAC sample testing. Therefore, the methodology developed in this study is expected to provide the reference for measuring the sorption/desorption isotherms of building materials with both static and automated techniques.

ABSTRACTInvasive pulmonary aspergillosis (IPA) continues to rise in concert with increasing numbers of immune suppression techniques to treat other medical conditions and transplantation. Despite these advances, morbidity and mortality rates remain unacceptably high. One strategy used to optimize outcomes is antifungal pharmacodynamic (PD) examination. We explored the pharmacodynamics of a new triazole in development, isavuconazole, in a murine neutropenic IPA model. TenA. fumigatusisolates were used, including four wild-type isolates and sixcyp51mutants. The MIC range was 0.125 to 8 mg/liter. Following infection, groups of mice were treated orally with the prodrug (BAL8557) at 40 to 640 mg/kg/12 h for 7 days. Efficacy was determined by quantitative PCR of lung homogenates. At the start of therapy, mice had 4.97 log10conidial equivalents (CE)/ml of lung homogenate, and this increased to 6.82 log10CE/ml of lung homogenate in untreated animals. The infection model was uniformly lethal in untreated control mice. The PD target endpoints examined included the static-dose AUC/MIC ratio and the 1-log10killing AUC/MIC ratio. A stasis endpoint was achieved for all isolates with an MIC of ≤1 mg/liter and 1-log10killing in all isolates with an MIC of ≤0.5 mg/liter, regardless of the presence or absence of thecyp51mutation. The static-dose range was 65 to 617 mg/kg/12 h. The corresponding median free-drug AUC/MIC ratio was near 5. The 1-log10killing dose range was 147 to 455 mg/kg/12 h, and the corresponding median free-drug AUC/MIC ratio was 11.1. These values are similar to those previously reported for other triazoles.

The mechanical properties of square section (up to 350 mm) forgings made of domestic steel VKS-9M (obtained by the duplex process of vacuum induction melting plus vacuum arc remelting) are studied. The mechanical properties of steel VKS-9M and American high-strength structural steel 300M are compared in terms of the calculated characteristics of materials. We extend the concept of «design characteristics of materials» to all the mechanical properties of materials used in determination of the strength and durability of structures. The design characteristics of materials include the strength characteristics under quasi-static tension, compression, crushing and shearing, as well as the strength under variable loads and the crack resistance of the material. The correctness of comparing the calculated values of the static strength of steels VKS-9M and 300M is based on the identity of test methods and the equivalence of processing and presentation of test results. The determination of the strength characteristics was carried out according to domestic standards, which are basically harmonized with the American standards. A difficult situation arises when determining the strength indicators of a newly developed material. Nowel material, as a rule, is developed in laboratory conditions, produced on experimental equipment in small volumes, and, therefore, the statistical assessment of the general population at the development stage is unreliable. In conditions of the full-scale manufacture of industrial semi-finished products, a statistical assessment of their strength characteristics becomes necessary. In the aviation industry, a statistical assessment of the calculated values of the strength characteristics of the material is prescribed by the law. The airworthiness standards set the levels of the calculated values of strength characteristics with a certain probability of non-destruction. For parts, the destruction of which can lead to an accident, the calculated values of the strength characteristics of materials should be selected in such a way that the strength of the material is to be ensured with a probability of 99% — with a 95% confidence interval (basis «A»). Statistical evaluation of the static strength characteristics of two heats of VKS-9M steel on the basis of «A» revealed their insignificant excess in comparison with steel 300M. The data obtained allow us to believe that the calculated values of the static strength characteristics of VKS-9M steel will not be lower than the calculated values for 300M steel. Large-scale fatigue tests of VKS-9M steel were carried out, which proved that the fatigue characteristics of VKS-9M and 300M steels practically coincide, as well as the arrays of their determinations. The equivalence of the strength characteristics of the VKS-9M and 300M steels allows replacement of the American 300M steel by the domestic VKS-9M steel.

ABSTRACTNonfermentative yeasts, such asCryptococcusspp., have emerged as fungal pathogens during the last few years. However, standard methods to measure their antifungal susceptibility (antifungal susceptibility testing [AST]) are not completely reliable due to the impaired growth of these yeasts in standard media. In this work, we have compared the growth kinetics and the antifungal susceptibilities of representative species of nonfermentative yeasts such asCryptococcus neoformans,Cryptococcus gattii,Cryptococcus albidus,Rhodotorulaspp.,Yarrowia lipolytica,Geotrichumspp., andTrichosporonspp. The effect of the growth medium (RPMI medium versus yeast nitrogen base [YNB]), glucose concentration (0.2% versus 2%), nitrogen source (ammonium sulfate), temperature (30°C versus 35°C), shaking, and inoculum size (103, 104, and 105cells) were analyzed. The growth rate, lag phase, and maximum optical density were obtained from each growth experiment, and after multivariate analysis, YNB-based media demonstrated a significant improvement in the growth of yeasts. Shaking, an inoculum size of 105CFU/ml, and incubation at 30°C also improved the growth kinetics of organisms. Supplementation with ammonium sulfate and with 2% glucose did not have any effect on growth. We also tested the antifungal susceptibilities of all the isolates by the reference methods of the CLSI and EUCAST, the EUCAST method with shaking, YNB under static conditions, and YNB with shaking. MIC values obtained under different conditions showed high percentages of agreement and significant correlation coefficient values between them. MIC value determinations according to CLSI and EUCAST standards were rather complicated, since more than half of isolates tested showed a limited growth index, hampering endpoint determinations. We conclude that AST conditions including YNB as an assay medium, agitation of the plates, reading after 48 h of incubation, an inoculum size of 105CFU/ml, and incubation at 30°C made MIC determinations easier without an overestimation of MIC values.

By means of the principle of virtual work, the governing equations together with the required boundary conditions of a higher-order shear deformation theory are formulated for the analysis of laminated shells under static loads. A system of 31 first-order partial differential equations is performed for the determination of stress resultants and displacement components. These equations are then solved numerically, utilizing the generalized differential quadrature method for two isotropic cylindrical panels with equal arc length but different radii having S2-type simply supported boundary conditions. The results matched those of other theories. Another analysis is carried out for composite cylindrical panels with two lamination schemes, five different mixed boundary conditions, and two length-to-thickness ratios. The results are compared against solutions obtained from ANSYS finite-element software.

The increase of power system demand leads to the change in voltage profile, reliability requirement and system robustness against disturbance. The voltage profile can be improved by providing a source of reactive power through the addition of new power plants, capacitor banks, or implementation of Flexible AC Transmission System (FACTS) devices such as Static VAR Compensator (SVC), Unified Power Flow Control (UPFC), Thyristor Controlled Series Capacitor (TCSC), Thyristor Controlled Phase Shifting Transformer (TCPST), and many others. Determination of optimal location and sizing of device injection is paramount to produce the best improvement of voltage profile and power losses reduction. In this paper, optimization of the combined advantages of TCPST and TCSC has been investigated using Particle Swarm Optimization (PSO) algorithm, being applied to the 30-bus system IEEE standard. The effectiveness of the placement and sizing of TCPST-TCSC combination has been compared to the implementation of capacitor banks. The result showed that the combination of TCPST-TCSC resulted in more effective improvement of system power losses condition than the implementation of capacitor banks. The power losses reduction of 46.47% and 42.03% have been obtained using of TCPST-TCSC combination and capacitor banks respectively. The TCPST-TCSC and Capacitor Bank implementations by using PSO algorithm have also been compared with the implementation of Static VAR Compensator (SVC) using Artificial Bee Colony (ABC) Algorithm. The implementation of the TCSC-TCPST compensation with PSO algorithm have gave a better result than using the capacitor bank with PSO algorithm and SVC with the ABC algorithm.

This article presents the results of comparative studies of existing methods to calculate the inductance of dynamic and static single-phase reactors of arc extinguishing by double-rod and armored magnetic circuits with non-magnetic gaps in terms of the geometric parameters of the device. There are proposed expressions to correct the determination of the effective area of the winding of reactors by the shunting magnetic flux. On the basis of numerical calculations, the significant influence of the correct calculation of the effective area of the reactor winding by the shunt magnetic flux on the result of calculating the inductance is shown, it undoubtedly affecting the accuracy of the operation of both many digital devices of existing substations in particular, and new digital substations being introduced in general. It was also found in the work, that carrying out the correct calculation will improve both electrical characteristics of the device, and technical economic indicators of capacitive current compensation systems as part of digital substations, when setting the inverse problem of determining the geometric dimensions of the electrical part of the reactor according to the known inductance value, which depends and is calculated on the parameters of the given network, including capacitive currents.

We propose large-diameter (160 mm) pre-cracked chevron notched Brazilian disc (P-CCNBD) specimens were used to study the asymmetric fracture law and determine the dynamic fracture toughness of rock. Specimens were diametrically impacted by a split Hopkinson pressure bar. The dynamic fracture failure process of each specimen was monitored by crack propagation gauges and strain gauges. Each of the large-diameter P-CCNBD specimens was found to exhibit prominent asymmetric fracture under impact load. The stress equilibrium condition cannot be satisfied. The dynamic fracture toughness values of the rocks were measured using the experimental-numerical method rather than the quasi-static method. The calculation results showed that the dynamic fracture toughness of rocks increases with the dynamic loading rate. In addition, at the 3-D crack front, the dynamic stress intensity factor was found be substantially different at each point. These data suggest that the dynamic fracture toughness of P-CCNBD specimens should be calculated by removing the value affected by an edge arc crack and taking the average value of the remaining points.

Abstract In order to produce three-dimensional components from metals, a wide variety of processes exist. Laser processes combined with metal powders are frequently used and developed. Restrictive factors are the machine-related small workspace, the machinery costs and the material portfolio, which place the technology in the area of high-performance components. Wire and arc additive manufacturing (WAAM), as a robust and economical welding process technology in combination with robot applications, represents an option to become more size-independent and provides variability in the range of materials. This work shows results for the robot-based WAAM of structures made from nickel alloy 617. The main focus of the investigation was the determination of the mechanical properties in the as-welded state for which static strength tests, microhardness and metallographic studies were carried out. The anisotropic material behaviour in relation to the build direction (BD) was tested. The direction-dependent strength properties of single-track welded structures are presented with samples taken and tested at 0°, 45° and 90° to the BD. The deformation behaviour was investigated by micro-tensile tests in a scanning electron microscope, whereby the formation of sliding steps on the polished surface under tensile stress was studied. The anisotropic behaviour of the WAAM structures is discussed under consideration of the microstructure and with regard to the grain size development and phase formation. The results indicate an anisotropic material behaviour in the as-welded state based of the crystallographic orientation of the material.

The Registrant Risk Assessment Scale (RRAS) was developed to aid New Jersey law enforcement representatives in assigning convicted sex offenders to risk tier classifications. The three risk tier classifications (low, moderate, and high risk) are linked to corresponding levels of community notification. The present study examined the scores of 574 adult males convicted of sex offenses in New Jersey on the seven RRAS items that assess static indicators of recidivism risk. Based on sentencing decisions, the sample included three groups of offenders: probationers, state prisoners, and those assigned to the Adult Diagnostic and Treatment Center (ADTC), an inpatient sex offender treatment facility for repetitive and compulsive offenders. An exploratory factor analysis of the RRAS items identified two important orthogonal factors: a forcible assault factor and a sexual deviance factor. One-way ANOVAs revealed significant differences among the three placement groups, with state prisoners tending to score highest on items reflecting antisocial orientation and forcible sexual assault and ADTC offenders scoring highest on items reflecting deviant sexual behavior. A discriminant analysis generated two distinct functions that classified well over half of the sample into their correct sentencing groups. Taken as a whole, the results provide preliminary support for the use of the RRAS in making sex offender risk determinations.

ABSTRACTMK7655 is a newly developed beta-lactamase inhibitor of class A and class C carbapenemases. Pharmacokinetics (PK) of imipenem-cilastatin (IMP/C) and MK7655 were determined for intraperitoneal doses of 4 mg/kg to 128 mg/kg of body weight. MIC and pharmacodynamics (PD) studies of MK7655 were performed against several beta-lactamase producingPseudomonas aeruginosaandKlebsiella pneumoniaestrains to determine its effectin vitroandin vivo. Neutropenic mice were infected in each thigh 2 h before treatment with an inoculum of approximately 5 × 106CFU. They were treated with IMP/C alone (every 2 hours [q2h], various doses) or in combination with MK7655 in either a dose fractionation study or q2h for 24 h and sacrificed for CFU determinations. IMP/MK7655 decreased MICs regarding IMP MIC. The PK profiles of IMP/C and MK7655 were linear over the dosing range studied and comparable with volumes of distribution (V) of 0.434 and 0.544 liter/kg and half-lives (t1/2) of 0.24 and 0.25 h, respectively. Protein binding of MK7655 was 20%. A sigmoidal maximum effect (Emax) model was fit to the PK/PD index responses. The effect of the inhibitor was not related to the maximum concentration of drug in serum (Cmax)/MIC, and model fits forT>MICand area under the concentration-time curve (AUC)/MIC were comparable (R2of 0.7 and 0.75), but there appeared to be no significant relationship of effect with dose frequency. Escalating doses of MK7655 and IMP/C showed that the AUC of MK7655 required for a static effect was dependent on the dose of IMP/C and the MIC of the strain, with a mean area under the concentration-time curve for the free, unbound fraction of the drug (fAUC) of 26.0 mg · h/liter. MK7655 shows significant activityin vivoand results in efficacy of IMP/C in otherwise resistant strains. The exposure-response relationships found can serve as a basis for establishing dosing regimens in humans.

The high level of injuries and the number of cases of death of law enforcement officers, especially in extreme situations, require constant and in-depth research in the field of improving the professional abilities of law enforcement officers. There is also no doubt about the practical significance, all units of the National Police from the stage of professional selection to the system of professional training need improvement and a systematic approach to increasing combat readiness and fighting capacity, qualitative changes in the system of professional training. Unfortunately, not every police officer is ready to act in certain difficult and extreme situations. The results of the study of the impact of extreme situations on the combat capability and combat readiness of police officers are presented. The main features of extreme situations and their impact on the readiness of police officers for effective actions are considered. Training of police officers is limited to physical, psychological and fire training in static (comfortable) conditions. Attention is paid to tactical readiness and readiness to act in extreme situations as the least researched areas of police training. The main professional qualities of police officers and ways to improve them are considered and analyzed. Determination and improvement of professional qualities of police officers should be carried out with the help of an improved methodology of tactical training. The main types of emotions that reduce the readiness of police officers to act in extreme situations and personal safety measures during dangerous actions are analyzed. The expediency of implementing emotional rehabilitation in preparing police officers for actions in extreme conditions has been established. The obtained results of the research indicate the high efficiency of the proposed approach and methods of increasing the combat readiness and combat capacity of police officers during actions in extreme situations.

ABSTRACT Telithromycin (HMR 3647) is a new ketolide that belongs to a new class of semisynthetic 14-membered-ring macrolides which have expanded activity against multidrug-resistant gram-positive bacteria. The aim of the present study was to investigate different basic pharmacodynamic properties of this new compound. The following studies of telithromycin were performed: (i) studies of the rate and extent of killing of respiratory tract pathogens with different susceptibilities to erythromycin and penicillin exposed to a fixed concentration that corresponds to a dose of 800 mg in humans, (ii) studies of the rate and extent of killing of telithromycin at five different concentrations, (iii) studies of the rate and extent of killing of the same pathogens at three different inocula, (iv) studies of the postantibiotic effect and the postantibiotic sub-MIC effect of telithromycin, and (v) determination of the rate and extent of killing of telithromycin in an in vitro kinetic model. In conclusion, telithromycin exerted an extremely fast killing of all strains of Streptococcus pneumoniae both with static concentrations and in the in vitro kinetic model. A slower killing of the strains of Streptococcus pyogenes was noted, with regrowth in the kinetic model of a macrolide-lincosamide-streptogramin B-inducible strain. The strains ofHaemophilus influenzae were not killed at all at a concentration of 0.6 mg/liter due to high MICs. A time-dependent killing was seen for all strains. No inoculum effect was seen for the strains of S. pneumoniae, with a 99.9% reduction in the numbers of CFU for all inocula at both 8 h and 24 h. The killing of the strains of S. pyogenes was reduced by 1 log10 CFU at 8 h and 2 to 3 log10 CFU at 24 h when the two lower inocula were used but not at all at 8 and 24 h when the highest inoculum was used. For both of the H. influenzae strains there was an inoculum effect, with 1 to 2 log10 CFU less killing for the inoculum of 108CFU/ml in comparison to that for the inoculum of 106CFU/ml. Overall, telithromycin exhibited long postantibiotic effects and postantibiotic sub-MIC effects for all strains investigated.

Abstract: Introduction: For the treatment of asymmetry of the midface due to permanent peripheral facial nerve palsy of different etiologies – which means a great psychical burden for the patients – several surgical solutions have been described. Aim: Our goal was to elaborate a minimally invasive surgical technique, that, on one hand, restores an adequate nasolabial fold, which is the most determinative keystone of facial symmetry. On the other hand, our technique can give an appropriate lift for the malar fat pad with shorter operative time and burden, with much lower complication rate and shorter recovery period compared to the classic static sling suspension techniques. Method: Out method is based on the formation of a neo-nasolabial fold, which is then suspended to the temporal fascia by permanent threads thus restoring facial symmetry and giving a lifting effect on the midfacial soft tissues as well. Results: Between 2014 and 2017, six patients had been operated with this nasolabial lifting technique of the malar fat pad without any major complications under local anesthesia on an outpatient basis at our Department. Conclusions: As our surgical exploration is minimal, the postoperative period is shorter, no visible scars remain on the face and the complication rate is negligible. If necessary, suspension could easily be adjusted, and as the technique is reversible, no other possible facial reconstruction methods are excluded. Our method can symmetrize the face in resting position completely on the long term, which is the most important issue for our patients according to their feedback. Orv Hetil. 2019; 160(22): 869–872.

Active magnetic bearings (AMBs) have the unique capability to act concurrently as support bearings and load cells for measuring shaft forces. Current state-of-the-art methods for force measurement rely on models with limited accuracy due to effects which are difficult to characterize such as fringing, leakage, and variations in material properties. In addition, these effects may be a function of actual air gaps which are difficult to determine in a dynamic operating environment. This paper discusses a new force measurement methodology that inherently accounts for these types of effects and other system uncertainties by utilizing multiple sets of current pairs in opposing actuators, in conjunction with a calculation algorithm, to accurately determine the force applied by the AMB. This new multipoint methodology allows for the determination of bearing forces from information on basic actuator geometry and control currents only, with no knowledge of actual operating air gaps required. The inherent nature of the methodology accounts for model uncertainties such as fringing, leakage, and other system unknowns. Initial static experimental test results are presented demonstrating 3% error in measuring the nominal determined bearing load, and a variation in calculated forces of less than 5% in most cases (8% in one case) when the location of the rotor within the bearing stator is modified. For the analogous conventional single-point measurements, the results show 15% error and 23% variation.

Effective sorbents are used to address the issues of improving food safety. The economic feasibility of cleaning increases when using food production wastes as sorbents. With the help of modern methods were studied the composition and properties of the sorbent ADC obtained from rice husk. Sorbent is a type of pyrogenic silica. With the help of modern methods for assessing the quality of substances, the chemical composition and morphological features of the surface of the sorbent were studied. In addition, the static exchange capacity for various pollutants was investigated. It is established that the ADC well detains inorganic compounds, which included iron, manganese and aluminum. Desorption of chemical compounds from the used sorbent does not have a significant effect on the content of heavy metals in water. This indicates the strength of sorption bonds. To establish the nature of the moisture in the sorbent with the determination of temperature intervals at which dehydration occurs, a simultaneous thermal analysis was carried out, which allowed determining the temperature zones that correspond to moisture removal, moisture removal with different binding energy, and predict the mode parameters of the moisture removal process and select the most effective way to remove them. dehydration. The operation of the device used is based on the continuous recording of the dependence of the change in mass of the material on time or temperature when it is heated in accordance with the selected temperature program in a given gas atmosphere. At the same time, the release or absorption of heat by the sample due to phase transitions or chemical reactions was recorded. Investigations were carried out under the following conditions: pressure — atmospheric, maximum temperature 600 K, rate of change of temperature 5 °C/min. The experiments were carried out in aluminum crucibles with a total weight of 10 mg. The analysis made it possible to identify periods of dehydration of water during thermal effects on the ADC, as well as to identify temperature zones that correspond to the release of moisture with different shapes and binding energy.

The article presents the analysis of the structure and properties of joints made of abrasion-resistant plates having the structure of chromium cast iron and welded with filler metals, the use of which aimed to provide the high abrasion resistance of the surface layer and good mechanical properties of the base material. The face layer of the joint was made using the MMA (Manual Metal Arc) welding method and the Fe-Cr-Nb-B type nanocrystalline filler metal. The root weld was welded using an austenitic filler metal, whereas the filling layer was welded using the MAG method with a low-alloy filler metal. The joints were subjected to non-destructive tests (visual tests and penetrant tests) as well as to mechanical properties tests. The research involved macro and microscopic metallographic tests, the determination of the grain size using an Xpert PRO X-ray diffractometer, and the EDS analysis of the chemical composition of the precipitates. The assessment of the operational properties of the joints based on hardness measurements, static tensile tests, bend tests as well as identifying the metal-mineral abrasive wear resistance were performed in accordance with ASTM G65 ? 04 standards. The results of the abrasion resistance tests were referred to the HARDOX 400 steel reference specimen. Considering the tests results it was concluded that the used filler materials can assure the appropriate operational properties of welded abrasion-resistant plates.

Abstract Objectives High sensitivity (hs) cardiac troponin (cTn) assays are defined per the IFCC Committee on Clinical Application of Cardiac Biomarker (C-CB) by the ability to measure ≥ 50% of concentrations greater than the limit of detection (LoD) with an impression of ≤10% at sex-specific 99th percentiles. Our study determined the sex-specific 99th percentile upper reference limits for males and females utilizing heparinized plasma from AACC universal sample bank for the Siemens point of care (POC) Atellica® VTLi hs-cTnI immunoassay. Methods Apparently healthy subjects, included overall 693, males 363, and females 330, following exclusionary surrogate biomarker use of hemoglobin A1c, NT-proBNP, and eGFR, along with statin medication. hs-cTnI was measured in a central laboratory, on multiple POC Atellica® VTLi immunoassay analyzers. The LoD was 1.24 ng/L and the 10%CV concentration was 6.7 ng/L. 99th percentile URLs were determined by the nonparametric (NP) method. Results Histograms of the hs-cTnI concentrations (ng/L) for males and females were used to visualize the distributions and concentrations in men and women and differed significantly (pre- and post-exclusion, both p <0.001). 99th percentile URLs were: overall 23 ng/L (90% CI 20–32 ng/L); male 27 ng/L (CI 21–37 ng/L); female 18 ng/L (CI 9–78 ng/L). The percentages of subjects having a measurable concentration ≥ the LoD were: overall 83.7%, male 87.3%, female 79.7%. Conclusions Our findings show the novel POC Atellica® VTLi hs-cTnI assay meets the designation of a ‘high-sensitivity’ assay using heparinized plasma.

The article discusses the main problems and features of test planning of serviceability of flexible production systems (FPS) equipment, taking into account all possible factors. The basic dependencies of repair and verification at variable operating modes have been monitored and possibl1e ranges of system existence depending on internal factors and changes in working time, environmental characteristics and factors affected the system have been derived. As the result of the analysis, mathematical modeling and determination of critical conditions of existence of the system have been carried out. The basic criteria and tolerances for the normal operation of FPS equipment have been determined. The calculated dependencies for the further improvement of the system and the parameters for the smooth operation of the equipment by means of a mathematical analysis have been found. Methods of test planning of operability of FPS equipment have been developed, the time frame of stopping various systems for the slightest l ss of production capacity, taking into account the duration of the diagnostic work of used equipment has been determined. The planning of inspections for a product that is in a state of readiness for action, possible only after replacement, is considered. These circumstances allow us to determine the optimal interval between inspections by the criterion of minimum expected costs, attributed to the average time of serviceability for the cycle in steady state. When planning inspections of a product that is in a state of static readiness for action, the possibility of making erroneous decisions during the inspection is taken into account. The products which start to act at occurrence of some emergency conditions which moments of occurrence are casual and have distribution W (t) are considered. It is determined that inspections and necessary repairs allow to increase readiness, if at the same time certain conditions concerning quantitative ratios of the sizes influencing it are fulfilled.

представлены результаты научно-исследовательских и опытно-конструкторских работ по разработке способов и оборудования, обеспечивающих подготовку элементов особотонкостенного трубопровода к их сборке в трассу. Созданы экспериментальная установка и рабочая оснастка для калибровки кольцевых стыков тонкостенных труб методами «раздача» или «обжатие» многосекторным инструментом, обеспечивающая отклонение наружного диаметра конца труб под автоматическую сварку кольцевых стыков не более ±0,15 мм от номинального размера. Экспериментально проверена теоретическая зависимость по определению критического давления обжатия, при котором стенка трубы теряет устойчивость и образуется гофр. Разработанное оборудование применялось при создании опытных образцов труб из титановых сплавов и коррозионностойких сталей с последующим натурным моделированием процессов сборки трубопровода. Сборка калиброванных труб проводилась при помощи автоматической аргонодуговой электросварки с предварительной фиксацией центрирующими хомутами и прихваткой. При оценке качества сварных стыков было установлено, что сварные кольцевые швы всех опытных образцов соответствуют требованиям нормативной технической документации. Поиск скрытых дефектов сварных соединений проводился с использованием акустико-эмиссионной системы и ультразвукового дефектоскопа. Проведенные испытания внутренним статическим и пульсирующим давлением показали высокую прочность и долговечность сварных стыков we present the results of research and experimental design work on the development of methods and equipment that ensure the preparation of elements of an extra-thin-walled conduit for their assembly into a route. We created an experimental setup and working equipment for calibrating the circumferential joints of thin-walled pipes using the methods of "expansion" or "compression" with a multi-sector tool, providing a deviation of the outer diameter of the pipe end for automatic welding of circumferential joints of no more than ±0,15 mm from the nominal size. We experimentally verified the theoretical dependence to determine the critical compression pressure at which the pipe wall loses stability and a corrugation is formed. The developed equipment was used to create prototypes of pipes from titanium alloys and corrosion-resistant steels, followed by full-scale modeling of pipeline assembly processes. We assembled the calibrated pipes by means of an automatic argon-arc welding with pre-fixation centering clamps and pre-welding. When assessing the quality of welded joints, we found that the welded circumferential seams of all prototypes comply with the requirements of regulatory technical documentation. We carried out the search for latent defects of welded joints using acoustic emission system and ultrasonic flaw detector. The tests carried out by internal static and pulsating pressure showed high strength and durability of welded joints

Abstract Kernite, ideally Na2B4O6(OH)2∙3H2O, is a major constituent of borate deposits and one of the most important mineral commodities of B. The chemical composition and crystal structure of kernite from the Kramer Deposit (Kern County, California) were investigated by a suite of analytical techniques (i.e., titrimetric determination of B content, gravimetric method for Na, ion selective electrode for F, high-T mass loss for H2O content, inductively coupled plasma atomic emission spectroscopy for REE and other minor elements, elemental analysis for C, N, and H contents) and single-crystal X-ray (at 293 K) and neutron (at 20 K) diffraction. The concentrations of more than 50 elements were measured. The general experimental formula of the kernite sample used in this study is Na1.99B3.99O6(OH)2∙3.01H2O. The fraction of other elements is, overall, insignificant: excluding B, kernite from the Kramer Deposit does not act as geochemical trap of other technologically relevant elements (e.g., Li, Be, or REE). The X-ray and neutron structure model obtained in this study confirms that the structure of kernite is built up by: two (crystallographically independent) triangular BO2OH groups and two tetrahedral BO4 groups, which share corner-bridging O atoms to form threefold rings, giving chains running along [010], and NaO4(OH)(OH2) and NaO2(OH)(OH2)3 polyhedra. Positional disorder of two H sites of H2O molecules was observed by the neutron structure refinement and corroborated by the maximum-entropy method calculation, which consistently provided a model based on a static disorder, rather than a dynamic one. The H-bonding network in the structure of kernite is complex, pervasive, and plays a primary role on its structural stability: the majority of the oxygen sites are involved in H-bonding, as donors or as acceptors. The potential utilizations of kernite, as a source of B (B2O3 ~50 wt%), are discussed, on the basis of the experimental findings of this study.

The paper suggests new interpretations of the meaning and structure of Anna Akhmatova’s early lyrics, in which “the moment of truth” for the heroine comes as if beyond consciousness, during movement and direct contemplation of the world. Free moving of a lyrical “self” by land and water either precedes the romance or provides a specific signal of a heroine’s spiritual rebirth afterwards. Thus, Akhmatova literally “goes beyond” usual woman’s poetic experience of love and separation: the heroine feels guilty about her earthly love; at the moment of a breakup she learns more about the pains of creativity and the joy of transforming the world with a religious feeling. Akhmatova works on the effect of psychological catharsis after experienced grief. The lyrical “self” stops active motion in space and gains static position, secluding herself at the end of 1912. We see the introducing of a theme of bodily illness, near-death hour and a death of a heroine in a state of external immobility. The colors of surrounding objects are suppressed as much as possible, things are discolored. Gazing into the distance is connected with Akhmatova’s experience of self-determination. Unconscious attraction of the lyrical “self” toward the open spaces of the “meagre” northern land precedes the entry of the historical theme into poet’s works. Stopping in space offers the heroine the sphere of subjective experience of movement in time, thus outlining the prospects of the epic Akhmatova’s view, future tragedy and heroics. The poet expands the boundaries of the lyrical heroine’s inner world largely due to the fact that she addresses the experience of a contemporary woman, for whom combining love and creativity in life is a source of a tragic experience. Not only Akhmatova’s poetic revelations of the first half of the 1910s are significant in view of a deep and subtle understanding of the woman’s world — they also act as a “key” to perceiving the tendencies and issues in Russian culture on the eve of the war and revolution.

Purpose The purpose of this paper is to describe a method permitting the creation of a realistic model of spherical roller bearing with the aim of determining contact stress and fatigue life based on dynamic loading conditions. The paper also aims to recognize the effect of tolerance values on contact stress and fatigue life. Motion and load transmission in spherical roller bearing occurs within the assembly by elliptical curved contacting surfaces. The stress produced by the transmitted load would be very high because of least contacting area between these surfaces. Design/methodology/approach The paper describes a methodology to determine contact stress using analytically as well as finite element method for spherical roller bearing. The comparison for the both each approach for contact stress at different loading condition is carried out. Prediction of fatigue life based on dynamic loading conditions for bearing is also determined using finite element model. The effect on induced contact stress and fatigue life by varying tolerances on inner race dimensions have been found out. Findings The paper suggests that the maximum stress produces at the start or end of the contacting arc under static loading condition in spherical roller bearing. The analytical and finite element approach is in good agreement. The fatigue life prediction is useful for selecting loading conditions for various applications of double row spherical roller bearing. Tolerance level at inner ring raceway radius is kept high because of manufacturing constrain of complex curvature geometric shape. Research limitations/implications The present approach does not consider dynamic loading conditions for contact stress analysis. Therefore, researchers are encouraged to analyze the effect of wear, lubrication and other tribological aspects on bearing life. Originality/value The paper includes determination of contact stress and prediction of fatigue life for spherical roller bearing using analytical as well as finite element approach. The tolerance values at inner race are identified as per manufacturing constraint based on contact stress and fatigue life.

<p class="MsoNormal" style="text-align: justify; line-height: 200%;">Axial bearing capacity (ABC) of piles is usually determined by static load test (SLT). However, conducting SLT is costly and time-consuming. High strain dynamic pile testing (HSDPT) which is provided by pile driving analyzer (PDA) is a more recent approach for predicting the ABC of piles. In comparison to SLT, PDA test is quick and economical. Implementing feed forward back-propagation artificial neural network (ANN) for solving geotechnical problems has recently gained attention mainly due to its ability in finding complex nonlinear relationships among different parameters. In this study, an ANN-based predictive model for estimating ABC of piles and its distribution is proposed. For network construction purpose, 36 PDA tests were performed on various concrete piles in different project sites. The PDA results, pile geometrical characteristics as well as soil investigation data were used for training the ANN models. Findings indicate the feasibility of ANN in predicting ultimate, shaft and tip bearing resistances of piles. The coefficients of determination, R², equal to 0.941, 0.936, and 0.951 for testing data reveal that the shaft, tip and ultimate bearing capacities of piles predicted by ANN-based model are in close agreement with those of HSDPT. By using sensitivity analysis, it was found that the length and area of the piles are dominant factors in the proposed predictive model.</p><p class="MsoNormal" style="text-align: justify; line-height: 200%;"> </p><p class="MsoNormal" style="text-align: justify; line-height: 200%;"><strong>Resumen</strong></p><p class="MsoNormal" style="text-align: justify; line-height: 200%;">La Capacidad Axial de Soporte (ABC, en inglés) de un pilote de construcción se determina usualmente a través de una Prueba de Carga Estática (SLT, inglés). Sin embargo, estas pruebas son costosas y demandan tiempo. La evaluación de las Dinámicas de Alto Esfuerzo de Pilotes (HSDPT, inglés), que la provee el programa de Análisis de Excavación (PDA, inglés), es una forma de aproximación más reciente para preveer la Capacidad Axial de Soporte. En comparación con la Prueba de Cargas Estática, la evaluación PDA es rápida y económica. La implementación de Redes Neuronales Arficiales (ANN, en inglés) que permita resolver problemas geotécnicos ha ganado atención recientemente debido a su posibilidad de hallar relaciones no lineales entre los diferentes parámetros. En este estudio se propone un modelo predictivo ANN para estimar la Capacidad Axial de Soporte de pilotes y su distribución. Para fines de una red de construcción se realizaron 36 pruebas PDA en pilotes de diferentes proyectos. Los resultados de los Análisis de Excavación, las características geométricas de los pilotes, al igual que los datos de investigación del suelo se utilizaron para probar los modelos ANN. Los resultados indican la viabilidad del modelo ANN en predecir la resistencia de los pilotes. Los coeficientes de correlación, R², que alcanzaron 0.941, 09.36 y 0.951 para la evaluación de los datos, revelan que la capacidad del pilotaje en el último rodamiento, en el cojinete del eje y en la punta que se predijeron con el modelo ANN concuerda con las establecidas a través del HSDPT. A través del análisis de respuesta se determinó que la longitud y el área de los pilotes son factores dominantes en el modelo predictivo propuesto.</p>

Railway bogie is the basic element that determines the force, kinematic, power and other parameters of the rolling stock, and its movement in the railway track has not been studied enough. Classical calculation of the kinematic and dynamic parameters of the bogie's motion with the determination of the position of its center of rotation, the instantaneous axes of rotation of wheelsets, the magnitudes and directions of all forces present a difficult problem even in quasi-static theory. The paper shows a simplified method that allows one to explain, within the limits of one article, the main kinematic and force parameters of the bogie movement (installation angles, clearance between the wheel flanges and side surfaces of the rails), wear and contact damage to the wheels and rails. Tribology of the railway bogie is an important part of transport tribology, the foundation of the theory of wheel-rail tribosystem, without which it is impossible to understand the mechanisms of catastrophic wear, derailments, contact fatigue, cohesion of wheels and rails. In the article basic questions are considered, without which it is impossible to analyze the movement of the bogie: physical foundations of wheel movement along the rail, types of relative motion of contacting bodies, tribological characteristics linking the force and kinematic parameters of the bogie. Kinematics and dynamics of a two-wheeled bogie-rail bicycle are analyzed instead of a single wheel and a wheelset, which makes it clearer and easier to explain how and what forces act on the bogie and how they affect on its position in the rail track. To calculate the motion parameters of a four-wheeled bogie, it is represented as two two-wheeled, moving each on its own rail. Connections between them are replaced by moments with respect to the point of contact between the flange of the guide wheel and the rail. This approach made it possible to give an approximate estimation of the main kinematic and force parameters of the motion of an ideal bogie (without axes skewing) in curves, to understand how the corners of the bogie installation and the gaps between the flanges of the wheels and rails vary when moving with different speeds, how wear and contact injuries arise and to give recommendations for their assessment and elimination.

The article focuses on the study of the phenomenon of syntactic complication of a simple sentence structure by semi-predicative adjectival components (traditionally known as detached participle and verbal adverb phrases) with the inherent potential predicativeness. The scientific relevance of the investigation is predetermined by a general tendency of modern linguistic studies towards the problems of semantic syntax, and by the attempt to elaborate a semantic theory of a sentence with regard to the central place of syntax in grammar, asymmetry of a language sign, deep categories of sentence. Therefore, the analysis of simple sentences complicated by semi-predicative adjectival components (SАC) is predefined foremost by their ability to distinguish priorities of a personality in his/her act of communication during which a speaker differentiates their propositions as dynamic or static, full-sentenced or elliptical. Semi-predicativeness is defined as a syntactic category of a simple (mono-predicative) complicated sentence, that has acquired formal features, namely, its structuring according to a certain grammatical pattern, where the participle, the verbal adverb, the adjective or the noun with an appositive function take a central position; its intonation of detachment; its syntactic autonomy and disjunction. In this respect, SAC is interpreted as embodied through the detachment of mono- or multi-lexeme syntactic formations appearing as a result of shortening of a simple elementary sentence into a non-sentence proposition. A central position in it is occupied by a secondary attributive syntaxeme represented by an adjective, that is grammatically and semantically related to a basic part of a simple complicated sentence and it illustrates secondary predicative-attributive relations (sometimes bearing adverbial features). The scientific novelty of the research results consists in the definition of SAC based on its formally-grammatical, semantic-syntactical and functional features; the determination of outer syntagmatic efficiency of the adjective in the structure of a simple complicated sentence; the highlighting of models that underline valency potential of adjectives within the limits of a semi-predicative construction, and also their distribution through valency-not-predefined components. The article explores and contains theoretical principles of the research of the adjective as a separate part of speech. Detachment and semi-predicativeness have been characterized as formally-syntactic categories of a simple sentence complicated by SAC; secondary predication has been analysed as a marker of semantic complication of simple sentences with SAC. Functional potential of semi-predicative adjectival components in the structure of a simple sentence has been described.

We hereby demonstrate a highly controllable, green, and rapid synthesis strategy for the production of binary metal sulfides on carbon nano-horns (CNHs) for the fabrication of non-enzymatic glucose sensor using commercial graphite screen-printed electrodes (GSPEs). CNHs were synthesized using the submerged arc discharge method as described elsewhere. In the next step, one droplet (50 µL) of the well-dispersed CNHs was carefully cast on the surface of the GCE (and GSPE). In the final step of preparing the modified electrodes, CNH/GCEs were covered by a thin layer of nickel cobalt sulfide by a simple electrochemical deposition procedure. Following the structural examination of the prepared electrocatalyst were examined. In this regard, several microscopic methods like FE-SEM or TEM were used to know more regard the morphology of the material. The FE-SEM images confirmed the successful formation of sponge like nanosheets of NiCo-S on carbonaceous material. Since CNHs are prepared from graphene sheets with open ends in horn-like shapes, they can be considered as being formed from the origami-like or crushing contortion of a single graphene sheet. According to the FE-SEM images, the agglomeration of CNHs establishes discrete nano-sphere structures with diameters in the range of 50 to 150 nm. The TEM images also confirm this nano-sphere strictures. Following detailed structural characterization, the electrocatalytic activity of the fabricated NiCo-S/CNH electrodes toward electro-oxidation of glucose is examined in detail. The cyclic voltammetry was also used for glucose electro-oxidation mechanism investigation. In addition, parameter optimization was also done by utilizing cyclic voltammetry technique. These include medium pH, deposition cycles, and metals mole ration. Moreover, the effect of the presence and absence of CNH on the electrochemical performance of glucose oxidation were also examined. It shows that in the presence of CNH the peak current density for glucose oxidation is higher that is perfect for analytical purposes and increase the analytical sensitivity. The structural properties and electrochemical response of the fabricated electrodes were also compared to their corresponding mono-metallic counterparts. The amperometric method was utilized as one of the most prevalent and momentous techniques for the quantitative determination of glucose. The applied potential in amperometric tests was optimized to be +0.5 V (vs. Ag/AgCl). The proposed electrodes were operated within two distinct linear dynamic ranges of 0.001- 0.330 mM and 0.330 - 4.53 mM of standard glucose with sensitivities of 1842 µA.mM-1.cm-2, and 854 µA.mM-1.cm-2, respectively. On the other hand, an analytical sensitivity of 467 µA.mM-1.cm-2, within the linear dynamic range of 0.5 - 6 mM was obtained for the static chronoamperometric measurements for modified commercial screen-printed electrode (GSPE). The proposed sensor showed outstanding selectivity against iso-structures and co-existing interferences, long-term durability, rapid response (1.7 s), excellent poisoning resistance against chloride ions, good repeatability, and reproducibility. The tested interferences were 0.1 mM solutions of fructose, galactose, sucrose, lactose, ascorbic acid, uric acid, dopamine hydrochloride, and NaCl. In order to demonstrate the capability of the fabricated electrodes and to evaluate their potential for glucose measurements in real physiological specimens, the glucose level was successfully measured in human blood serum, urine, and saliva samples, without any sample pre-treatment. All in all, the encouraging results make NiCo-S/CNH electrodes immensely promising for clinical and physiological glucose-level measurements. Figure 1. (a) FE-SEM image of CNH. (b) FE-SEM image of NiCo-S/CNH. (c) TEM image of CNHs. (d) Cyclic voltammogarm of NiCo-S/CNH in the absence and presence of various concentration of glucose. (e) The amperometric responses of the NiCo-S/CNH/GCEs after addition of 1 mM glucose followed by successive injection of 0.1 mM fructose, galactose, sucrose, lactose, AA, UA, DA, and NaCl. (f) Chrono amperograms of the NiCo-S/CNH/GSPEs in the presence of the different glucose concentration ranging from 0.5 ~ 10.0 mM under applying +0.5 V. Amperogram of the NiCo-S/GCEs current response obtained from the addition of (g) 50 µL human blood serum, (h) 200 µL urine, and (i) 100 µL saliva followed by injection of 400 µM standard glucose solutions under the optimum potential of +0.5 V, the amperograms of five successive glucose addition. Figure 1

The present volume marks the completion of a large research project by the Geological Survey of Denmark and Greenland (GEUS), focused on the northern part of the Palaeoproterozoic Nagssugtoqidian orogen of central West Greenland, and carried out by a team of Danish and international participants. The project comprised geological mapping as well as structural, geochronological, geochemical and economic geological studies. This volume contains reports on both Archaean and Palaeoproterozoic geology as well as a study of neotectonic brittle structures. The field work was carried out in 2000-2003 in the region between Nordre Strømfjord and Jakobshavn Isfjord (see e.g. van Gool & Piazolo 2006, this volume, fig. 1). The project had two immediate purposes, namely to establish an overview of the mineral resource potential of supracrustal rocks in the region between 66° and 70°15'N, and produce four new geological sheets in the Survey's 1:100 000 map series. The first collection of papers about the Nagssugtoqidian orogen, published by the Geological Survey of Greenland (GGU, now part of GEUS), dates back to 1979 (Korstgård 1979). The investigations in this period were mainly based on field descriptions and structural analysis of coastal areas in the southern and central parts of the orogen, combined with limited petrographical, palaeomagnetic and geochronological studies; the results also comprised the first 1:100 000 geological map from within the Nagssugtoqidian orogen (Olesen 1984). The Proterozoic age of the orogen had been established, but it was believed that most, if not all of the quartzofeldspathic basement gneisses were of Archaean origin. Subsequent work in the Nagssugtoqidian orogen by GGU in the 1980s showed that besides Archaean orthogneisses and supracrustal rocks, the central part of the orogen also comprises the root zone of a Palaeoproterozoic magmatic arc and associated panels of Palaeoproterozoic volcanic and metasedimentary rocks (Kalsbeek et al. 1987). These results were confirmed during further investigations by the Danish Lithosphere Centre (DLC) in 1994-1999, and the plate-tectonic collisional history of the southern and central Nagssugtoqidian orogen was described in detail (van Gool et al. 2002). However, these studies added little to previous knowledge of the northern parts of the orogen in the Kangaatsiaq-Aasiaat-Qasigiannguit region, knowledge that was largely based on coastal reconnaissance by Henderson (1969) at the time when the entire orogen was still believed to consist of Archaean rocks. Another project preceding the present work was carried out by GGU in 1988-1991 immediately north of the Nagssugtoqidian orogen, in the southernmost part of the likewise Palaeoproterozoic Rinkian fold belt (Disko Bugt project, Kalsbeek 1999). It was shown that also the latter region comprises Palaeoproterozoic (meta)sedimentary rocks, and that most of the Archaean basement is strongly overprinted by Palaeoproterozoic structures that were formed during overall W- or NW-directed lateral tectonic transport. Although these structures might be related to similar structures in the Nagssugtoqidian orogen, the relationship between the Nagssugtoqidian orogen and the Rinkian fold belt remained speculative. The only previous economic geological study of regional extent in central West Greenland was an airborne reconnaissance study supplemented by local field work, which was carried out in the early 1960s by Kryolitselskabet Øresund A/S. This work resulted in the discovery of a massive sulphide deposit at Naternaq (Lersletten), which was studied again in some detail in 2001 by the Survey (Østergaard et al. 2002) but not reported on in the present volume. The present volume comprises 12 papers with topics ranging geochronologically from mid-Archaean to Palaeogene, and geographically from the southern Nagssugtoqidian foreland to the central part of the Rinkian fold belt. Many of the papers deal with the northern part of the Nagssugtoqidian orogen and are related to the recent field work in that region, while a few contributions are rooted in DLC- or other projects. The papers have been arranged in approximate chronological order and are grouped in terms of their main subjects. The two first papers, by Hollis et al. and Moyen & Watt, deal with Archaean supra- and infracrustal rocks in the northern Nagssugtoqidian orogen: their origin, ages, and structural and metamorphic evolution. These papers provide insight into the age and origin of the continental crustal orthogneisses and granites that underlie most of the region, and discuss the relationships between the supracrustal and plutonic components, using zircon U-Pb age determinations and major and trace element geochemical characteristics. Also the question of Palaeoproterozoic tectonic overprint is discussed, with the conclusion from both study areas that most of the observed structures are Archaean. The third paper with focus on Archaean geology, by Stendal et al., describes a small gold prospect at Attu likewise in the northern Nagssugtoqidian orogen, and discusses the age of the prospect and its host rocks using Pb-Pb geochronology of magnetite. It is concluded that the host rocks at Attu may be as old as 3162 ± 43 Ma, and that the gold prospect itself is around 2650 Ma in age. The fourth paper, by Mayborn & Lesher, is a thorough review of the Kangâmiut dyke swarm in the southern Nagssugtoqidian orogen and its foreland. It includes new whole-rock and mineral chemical data, and a list of sampling sites and corresponding field data. The emplacement mechanism and depth of the dyke swarm are discussed in detail, and it is concluded that the dykes were emplaced during the initial rifting prior to the Nagssugtoqidian collision and that they are unrelated to subduction processes (contrary to the belief by some previous authors). The next three papers provide geochronological constraints on the ages of supra- and infracrustal rocks and the deformation and metamorphism in the northern Nagssugtoqidian orogen, and on late orogenic uplift in the central Rinkian fold belt. In the first of these papers Thrane & Connelly employ zircon U-Pb age determinations (mainly using the laser ICP-MS method), and for the first time provide unequivocal documentation that the Naternaq supracrustal belt is of Palaeoproterozoic age. Other zircon age data from a synkinematic granite southeast of Kangaatsiaq show that the large fold structures in this region are of Archaean age. The subsequent paper by Stendal et al. presents Pb-Pb ages and isotopic signatures of magnetite in amphibolites; the obtained ages are younger than 1800 Ma and are related to cooling of the orogen. Stepwise leaching Pb-Pb ages of monazite and allanite in pegmatites fall in the range of 1750-1800 Ma, and are interpreted to date the emplacement of these rocks. The third paper in this group, by Sidgren et al., deals with new 40Ar/39Ar ages of around 1790 Ma (hornblende) and 1680 Ma (muscovite) from Archaean and Palaeoproterozoic rocks in the central Rinkian fold belt, which are interpreted as orogenic cooling ages. The hornblende ages are significantly older than such hornblende ages previously obtained from the central and northern Nagssugtoqidian orogen, pointing to different uplift histories in the two regions. This may in turn suggest that the Rinkian continental collision preceded that in the Nagssugtoqidian orogen. Four of the remaining five papers deal with the Nagssugtoqidian structural evolution. In the first of these, van Gool & Piazolo present a new method of structural analysis, where a geographical information system (GIS) is used as a framework for visualisation and analysis of large amounts of structural data. The paper graphically presents an overview of thousands of data points within an area of approximately 160 × 180 km in the central and northern parts of the Nagssugtoqidian orogen. This interesting data set points directly towards the two next papers, where crustal-scale structures in the same region and their origin are discussed: Sørensen et al. address the prominent Nordre Strømfjord shear zone just south of this block, and describes the structural and metasomatic transition into the shear zone by means of aeromagnetic and lithological map patterns and geochemical data. Another paper, by Mazur et al., addresses a prominent break in the structural pattern within the Kangaatsiaq-Aasiaat area, where the southern part acted as a rigid block during the Nagssugtoqidian orogeny and thus preserved its Archaean structure. The fourth paper in this group, by Korstgård et al., combines rock and aeromagnetic data to discuss the relationship between structure, metamorphic facies and total magnetic field intensity anomalies in the southern Nagssugtoqidian orogen. The authors show that static metamorphic boundaries are gradual, whereas boundaries along deformation zones are abrupt. The last paper, by Wilson et al., is a novel remote sensing and field geological analysis of onshore brittle structures related to the complex Ungava fault zone in the Davis Strait, which developed during the Cretaceous-Palaeogene opening of the Labrador Sea - Davis Strait - Baffin Bay seaway. The study area is located in the central Nagssugtoqidian orogen, and the authors carefully establish a distinction between old Nagssugtoqidian and younger structures in the basement rocks and identify five main sets of young lineaments. They conclude that the onshore fault patterns are predominantly of strike-slip nature, and that they reflect the stress fields that governed the opening of the seaway. Acknowledgements The editors are grateful to the 14 external reviewers, each of whom reviewed one or more of the individual papers, for their thorough and constructive work.

Objective: The main objective was to develop simple, cost-effective, rapid and selective spectrophotometric methods for the determination of atorvastatin calcium and pitavastatin calcium in pure and pharmaceutical formulations using acid dyes like bromothymol blue, bromocresol purple and bromocresol green and also in human urine samples. Methods: The developed methods were based on the formation of ion-pair complexes between statin drugs and acid dyes after studying the optimization conditions. The association constants of the developed ion-pair complexes were evaluated using Benesi–Hildebrand equation. The methods were validated according to ICH guidelines. Results: The formed ion-pair complexes showed maximum absorbance which was measured at 637 nm for both methods A and D, 601 nm, 606 nm for methods B and E and 631 nm for both methods C and F respectively with correlation coefficients 0.999. The analytical parameters and their effects in the developed methods were investigated. The ion-pair complexes were stable up to 24 h and showed good linearity. The molar absorptivity, Sandell sensitivity, detection, and quantification limits were also calculated. The stoichiometry ratio in all the cases was 1:2 by using Job’s method of continuous variation. The recovery studies again showed good results because co-formulated substances did not interfere for the determination of ATC and PTC in the developed methods. Conclusion: The developed methods were applicable for routine quality control analysis of ATC and PTC in pure and pharmaceutical dosage forms. Good results were obtained when the developed methods were applied in healthy human urine samples.

Preference for augmentative or alternative communication (AAC) systems has received growing interest in work with individuals with developmental disabilities. An individual may choose a modality based on technological (e.g., auditory-output) or aesthetic features of a system; however, it is ideal that functional features (i.e., effectiveness in producing a reinforcer) affect preference to a much greater extent. Prior research has treated preference as a static variable and may commonly report a lack of preference for a modality or control by irrelevant features of the assessment (e.g., position of the modality in an array). The current study assessed the preference for AAC modalities of a teenager with autism spectrum disorder and intellectual disability using a concurrent-chains procedure. This study extended prior research by including additional methods to ensure accurate assessment of preference (i.e., a control condition) and a reinforcer manipulation to determine whether preference was controlled by non-functional (e.g., aesthetic) or functional (i.e., reinforcer quality and availability) variables. Preference was found to be functionally related to reinforcer availability, including when rapidly alternated between modalities. Moreover, the participant consistently allocated responding away from the control condition. Implications for self-determination and suggestions for future research on preference for AAC systems are considered.

Nonlinear viscoelastic behavior is a characteristic of many engineering materials including flexible polyurethane foam, yet it is difficult to develop dynamic models of systems that include these materials and are able to predict system behavior over a wide range of excitations. This research is focused on a specific example system in the form of a pinned-pinned beam interacting with a viscoelastic foundation. Two cases are considered: (1) the beam and the foundation are glued so that they are always in contact and the foundation can undergo both tension and compression, and (2) the beam is not glued to the foundation and the foundation reacts only in compression so that the contact region changes with beam motion. Static as well as dynamic transverse and axial forces act on the beam, and the Galerkin method is used to derive modal amplitude equations for the beam-foundation system. In the second case of the beam on tensionless foundation, loss of contact between the beam and the foundation can arise and determination of the loss-of-contact points is integrated into the solution procedure through a constraint equation. The static responses for both cases are examined as a function of the foundation nonlinearity and loading conditions. The steady-state response of the system subject to static and harmonic loads is studied by using numerical direct time-integration. Numerical challenges and the accuracy of this approach are discussed, and predictions of solutions by the three-mode and five-mode approximate models are compared to establish convergence of solutions. Frequency responses are studied for a range of foam nonlinearities and loading conditions.

ABSTRACT Antimicrobial resistance among uropathogens has increased the rates of infection-related morbidity and mortality. Antofloxacin is a novel fluoroquinolone with broad-spectrum antibacterial activity against urinary Gram-negative bacilli, such as Escherichia coli. This study monitored the in vivo efficacy of antofloxacin using bioluminescent imaging and determined pharmacokinetic (PK)/pharmacodynamic (PD) targets against E. coli isolates in a neutropenic murine thigh infection model. The PK properties were determined after subcutaneous administration of antofloxacin at 2.5, 10, 40, and 160 mg/kg of body weight. Following thigh infection, the mice were treated with 2-fold-increasing doses of antofloxacin from 2.5 to 80 mg/kg administered every 12 h. Efficacy was assessed by quantitative determination of the bacterial burdens in thigh homogenates and was compared with the bioluminescent density. Antofloxacin demonstrated both static and killing endpoints in relation to the initial burden against all study strains. The PK/PD index area under the concentration-time curve (AUC)/MIC correlated well with efficacy (R 2 = 0.92), and the dose-response relationship was relatively steep, as observed with escalating doses of antofloxacin. The mean free drug AUC/MIC targets necessary to produce net bacterial stasis and 1-log10 and 2-log10 kill for each isolate were 38.7, 66.1, and 147.0 h, respectively. In vivo bioluminescent imaging showed a rapid decrease in the bioluminescent density at free drug AUC/MIC exposures that exceeded the stasis targets. The integration of these PD targets combined with the results of PK studies with humans will be useful in setting optimal dosing regimens for the treatment of urinary tract infections due to E. coli.

ABSTRACT While isoniazid and rifampin have been the cornerstone of tuberculosis therapy caused by drug-susceptible Mycobacterium tuberculosis for more than 40 years, their combined action has never been thoroughly assessed by modern quantitative pharmacology approaches. The aims of this work were to perform in vitro experiments and mathematical modeling of the antibacterial effect of isoniazid and rifampin alone and in combination against various strains of Mycobacterium tuberculosis. After MIC determination of H37Rv and three strains belonging to the Beijing, Euro-American, and Indo-Oceanic lineages, the antibacterial effects of isoniazid and rifampin alone and in combination were studied in static time-kill experiments. A sigmoidal maximum effect model (Hill equation) and a response-surface model were used to describe the effect of the drugs alone and in combination, respectively. The killing effect of isoniazid and rifampin alone were well described by the Hill equation. Rifampin displayed a more concentration-dependent effect than isoniazid around the MIC. The pharmacodynamics parameters of each drug (maximal effect, median effect concentration, and coefficient of sigmoidicity) were quite similar between the four strains. The response-surface model from Minto et al. fit data of combined effect very well with low bias and imprecision (C. F. Minto, T. W. Schnider, T. G. Short, K. M. Gregg, A. Gentilini, Anesthesiology 92:1603–1616, 2000, https://doi.org/10.1097/00000542-200006000-00017). Response-surface modeling showed that the combined action of isoniazid and rifampin was synergistic for the H37Rv, Beijing, and Euro-American strains but only additive for the Indo-Oceanic strain. This study can serve as a motivating example for preclinical evaluation of combined action of antituberculous drugs.

Oyo state like most of the other states in the Nigeria has an old and conventional Geodetic Network. This network was established and computed using the conventional method of position determination and the science of navigation in the 1930- 1950's which is based on the 30th arc-meridian of the Clarke 1880 modified ellipsoid with its origin at L40 Minna datum. This does not satisfy the overgrowing mapping requirements of the state and as well as other Geo-related information requirements. The required data were acquired with GNSS receivers set up on each of the existing points in static mode. The reference network that was re- established consists of eight (8) primary points, covering an area of approximately ?12km?^2 with an average separation of about 7Km. The new network monuments are made of reinforced concrete, solidly cemented in the ground with metal caps (brass markers), and coordinated using the static carrier phase differential GPS measurement. Several observation sessions were conducted and processed to compute 3D coordinates for the network. Post processed data were rigorously adjusted using Least Squares and must meet the distance accuracy specifications for a first order network (1:100,000). Final coordinates are based on WGS84 frame/ ITRF 2008 datum and are within a mean accuracy of ±0. 05m. Finally, the plots of the re-established control points were presented and thus re-established a reliable first order geodetic reference network within Oyo state. Based on the findings the study recommends all geodetic networks in Nigeria should be re- established.

AbstractGlobal Navigation Satellite Systems (GNSS) Real-time Precise Point Positioning (RT-PPP) strongly relies on the precise satellite orbits and clocks, especially the latter requires high update rate, e.g., five seconds, due to its limited prediction accuracy. Such a high-rate update frequency is a burden to both the data analysis and communicating, and interruption in communicating is almost unavoidable. For the new constellations such as Galileo and BDS-3 with high-stability hydrogen clocks onboard, it is possible to predict satellite clocks to a longer arc. Moreover, recent developments in multi-GNSS ultra-rapid precise orbit determination enables the half-hourly update, ensuring the availability with a prediction arc of 30–60 min. We investigate multi-GNSS RT-PPP using half-hourly predicted products and demonstrate that a 3-D accuracy of 2.9 and 11.3 cm can be achieved for static and simulated kinematic solutions, respectively. We present the different clock prediction accuracies of different types of satellites and propose a satellite-specific weighting strategy in PPP, which exploits the benefits of the satellites of good performance. The method is based on the prediction accuracy of both, satellites and clocks, and shows an improvement of 15 to 60% compared to those without satellite-specific weighting or with simplified weighting strategies. We also demonstrate that Galileo satellites contribute the most in the quad-constellation solution, thanks to the highly stable satellite clocks.

Static performance characteristics and rotordynamic coefficients were experimentally determined for a four-pad, ball-in-socket, tilting-pad journal bearing in load-between-pad configuration. Measured static characteristics include journal static equilibrium position, estimated power loss, and trailing-edge pad temperatures. Rotordynamic coefficients were determined from curve-fits of measured complex dynamic-stiffness coefficients as a function of the excitation frequency. Aside from the cross-coupled damping coefficients, a frequency-independent [M]-[C]-[K] model did a good job of fitting the measurements. The added-mass coefficient was frequently dropped, leaving only a frequency-independent stiffness and damping coefficient. Test conditions included speeds from 4000 rpm to 12,000 rpm and unit loads from 0 kPa to 1896 kPa (0–275 psi). The bearing uses cool inlet oil to decrease the pad operating temperatures and increase the bearing’s load and speed capacity. The bearing has a nominal radial clearance of 95.3 μm (3.75 mils). However, measurements indicated significant bearing crush with a radial bearing clearance of 99.6 μm (3.92 mils) in the axis 45 deg counterclockwise from the loaded axis and 54.6 μm (2.15 mils) in the axis 45 deg clockwise from the loaded axis (assuming counterclockwise rotation). The pad length is 101.60 mm (4.00 in.), giving L/D=1.00. The pad arc angle is 73 deg, and the pivot offset ratio is 65%. Testing was performed using a test rig described by Kaul (1999, “Design and Development of a Test Setup for the Experimental Determination of the Rotordynamic and Leakage Characteristics of Annular Bushing Oil Seals,” MS thesis, Texas A&M University, College Station, TX), and rotordynamic coefficients were extracted using a procedure adapted from the work of Childs and Hale (1994, “A Test Apparatus and Facility to Identify the Rotordynamic Coefficients of High-Speed Hydrostatic Bearings,” ASME J. Tribol., 116, pp. 337–344). A bulk-flow Navier–Stokes model was used for predictions, using adiabatic conditions for the fluid in the bearing. However, the model assumes constant nominal clearances at all pads, and an average clearance was used based on measured clearances. Measured static eccentricities and attitude angles were significantly higher than predicted. Attitude angles varied from 6 deg to 39 deg and decreased with load. Power loss was underpredicted at low speeds and very well predicted at high speeds, with a maximum value of 25 kW (34 hp). The maximum detected pad temperature was 71°C(160°F) while the temperature increase from inlet to maximum pad temperature location was overpredicted by 10–40%. Direct stiffness and damping coefficients were significantly overpredicted, but the addition of a stiffness-in-series correction vastly improved the agreement between theory and experiment. Direct added masses were zero or negative at low speeds and increased with speed up to a maximum of about 50 kg; they were normally greater in the x (unloaded) direction. Although significant cross-coupled stiffness terms were present, they always had the same sign, and the bearing had a whirl frequency ratio of zero netting unconditional stability over all test conditions.

Bacterial tyrosine kinases (BY-kinases) comprise a family of protein tyrosine kinases that are structurally distinct from their functional counterparts in eukaryotes and are highly conserved across the bacterial kingdom. BY-kinases act in concert with their counteracting phosphatases to regulate a variety of cellular processes, most notably the synthesis and export of polysaccharides involved in biofilm and capsule biogenesis. Biochemical data suggest that BY-kinase function involves the cyclic assembly and disassembly of oligomeric states coupled to the overall phosphorylation levels of a C-terminal tyrosine cluster. This process is driven by the opposing effects of intermolecular autophosphorylation, and dephosphorylation catalyzed by tyrosine phosphatases. In the absence of structural insight into the interactions between a BY-kinase and its phosphatase partner in atomic detail, the precise mechanism of this regulatory process has remained poorly defined. To address this gap in knowledge, we have determined the structure of the transiently assembled complex between the catalytic core of the Escherichia coli (K-12) BY-kinase Wzc and its counteracting low–molecular weight protein tyrosine phosphatase (LMW-PTP) Wzb using solution NMR techniques. Unambiguous distance restraints from paramagnetic relaxation effects were supplemented with ambiguous interaction restraints from static spectral perturbations and transient chemical shift changes inferred from relaxation dispersion measurements and used in a computational docking protocol for structure determination. This structurepresents an atomic picture of the mode of interaction between an LMW-PTP and its BY-kinase substrate, and provides mechanistic insight into the phosphorylation-coupled assembly/disassembly process proposed to drive BY-kinase function.

Wave-piercing catamaran hull forms are widely used for high-speed ferry applications due to the hull slenderness, suitable for achieving high speeds. The global loads acting on these craft are of great interest as there is limited knowledge on determining the magnitude of the loads, in particular when operating in random sea conditions. Longitudinal and transverse bending moments as well as pitch connecting moments and hull torsion loads act on the hull simultaneously. This paper investigates the estimation of these global loads from full-scale catamaran sea trials strain gauge data using finite element methods. Det Norske Veritas (DNV) load cases are applied to a finite element model in order to determine the conversion between local strain values observed during sea trials and prevailing global loads. Comparisons are thus made of global loads determined from strain data collected from sea trials with DNV global load cases. The results show that this method is relatively reliable for the prediction of hull global loads in the absence of slamming. Comparisons have been made for different heading angles. The quasi-static design loads are important during the ship design stage, as they are good proxies in wavelengths comparable to the hull length for rationally determined loads obtained from a first-principles dynamic analysis. The broad aims here are to demonstrate the use of strain sensor data obtained during sea trials for determination of global sea loads, to reconcile the loads thus determined with DNV load cases and thereby to improve the accuracy of the predicted loads used in design to increase the structural efficiency of vessel design.

Wave-piercing catamaran hull forms are widely used for high-speed ferry applications due to the hull slenderness, suitable for achieving high speeds. The global loads acting on these craft are of great interest as there is limited knowledge on determining the magnitude of the loads, in particular when operating in random sea conditions. Longitudinal and transverse bending moments as well as pitch connecting moments and hull torsion loads act on the hull simultaneously. This paper investigates the estimation of these global loads from full-scale catamaran sea trials strain gauge data using finite element methods. Det Norske Veritas (DNV) load cases are applied to a finite element model in order to determine the conversion between local strain values observed during sea trials and prevailing global loads. Comparisons are thus made of global loads determined from strain data collected from sea trials with DNV global load cases. The results show that this method is relatively reliable for the prediction of hull global loads in the absence of slamming. Comparisons have been made for different heading angles. The quasi-static design loads are important during the ship design stage, as they are good proxies in wavelengths comparable to the hull length for rationally determined loads obtained from a first-principles dynamic analysis. The broad aims here are to demonstrate the use of strain sensor data obtained during sea trials for determination of global sea loads, to reconcile the loads thus determined with DNV load cases and thereby to improve the accuracy of the predicted loads used in design to increase the structural efficiency of vessel design.

Background & Objective: Determination of hemoglobinopathies is significant for epidemiological studies. There is a need to identify burden of hemoglobinopathies at national level to lay down the foundation of appropriate screening and prevention programs. The present study aimed to evaluate the spectrum of hemoglobinopathies along with hematological and biochemical parameters in a tertiary care hospital. Methods: This retrospective study included results of high performance liquid chromatography (HPLC) test from July 2015 - May 2020 in the department of Hematology, Indus Hospital and Health Network, Karachi, Pakistan. Data of all patients collected for red blood cell (RBC) indices, serum iron profile, and vitamin B12 and red cell folate levels. Diagnosis of hemoglobinopathies was done by an automatic analyzer ADAMS A1C Model No. HA-8180T Arkray/Japan. Results: Among 2422 participants, hemoglobinopathy observed in 14.5% (n=352). Beta thalassemia trait is observed as the most common hemoglobinopathy (6.4%). Severe anemia (Hb=5.1-5.5 g/dl) found in beta thalassemia major (BTM) and HbE disease. Red cell parameters showed significant association with different types of hemoglobinopathies. Mean ferritin level was high in E-beta thalassemia (687.8±591.9) followed by sickle cell disease (615.7±543.5). Conclusion: Apparently, overall frequency is static however, results of this study are not applicable to general population due to sample bias. Moreover, true figures are difficult to identify due to high incidence of iron deficiency anemia that masks the diagnosis by conventional techniques. Molecular characterization by DNA analysis is the most reliable tool of diagnosis. However, this method is not widely available in our country due to lack of expertise and cost issues. doi: https://doi.org/10.12669/pjms.38.8.5935 How to cite this:Mansoor N, Meraj F, Shaikh A, Jabbar N. Spectrum of hemoglobinopathies with hematological and biochemical profile: A five year experience from a tertiary care hospital. Pak J Med Sci. 2022;38(8):---------. doi: https://doi.org/10.12669/pjms.38.8.5935 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

The following essay constitutes a theoretical journey through the landscape of the NAMES Project AIDS Quilt, one narrated from the perspective of those who live on to mourn, to remember. To some critics, my approach might at once appear radical or unorthodox since I focus on how Quilt spectators engage with the artifact and are thereby implicated in the history of the epidemic, rather than on how the dead are made to speak from beyond the grave. My intent is not to invalidate the claims made or the conclusions reached by other critics who have written persuasively about how the Quilt facilitates a voice (both individual and collective) for those who have died of AIDS-related illnesses; indeed, such critics have contributed a wealth of significant knowledge to critical understandings of the pedagogical and political functions of the Quilt. My intent, rather, is to respond to a set of as yet unexplored questions about how the ever-evolving landscape of the AIDS epidemic has altered the nature and purpose of Quilt spectatorship. When the Quilt was created in 1985, “naming names” was an important strategy for political survival, especially given the institutional apathy that silenced and marginalized all who were infected and/or affected by the epidemic. However, over the past sixteen years, apathy has slowly given way to increased attention by medical and media institutions. No longer is memory and reverence enough. Now, we must ask ourselves how the Quilt can continue to be used to combat the emergent obstacles that have sprung up in the wake of apathy and silence. This is not to suggest that remembrance, mourning, and reverence are not still significant responses to the epidemic; we cannot forgot the past, lest we repeat it. But it is to suggest that as we look back, we also must move forward and continue to chart new horizons for how our minds and bodies engage with the Quilt as a social and political space. Throughout this essay, then, my conclusions are at best tentative, offered more as a gesture of hope than as a model for survival. It is my hope that critics can continue to press against social spaces both with caution and determination because those actions matter. We must act with caution because there can be devastating consequences of asserting claims to visibility and location. We must act with determination because there are equally perilous consequences of not doing so. Since its meager beginnings, the NAMES Project AIDS Quilt has undergone exponential changes in size, shape, and scope, but critical responses to the Quilt have remained stagnant with most critics attributing to the Quilt a single meaning and purpose: to revere the dead. Cultural critic Peter S. Hawkins, for instance, argues that "the Quilt . . . is most profoundly about the naming of names" (760), while journalist Jerry Gentry suggests that the Quilt bespeaks "a national and international constructive expression of grief" (550), a grief which most powerfully resonates in the loss of individual lives. While "naming names" is a politically important function of the Quilt, critics who read the artifact as only motivated by memory assume that exhibitions of the artifact facilitate a static model of performance. For such critics, the Quilt represents a mass graveyard--both as a place of interment and as a place in which ideological meanings are circumscribed by the fixity and stillness of reverence. (This reading is partly enabled by the fact that each panel measures the size of a human grave.) Not only does this reading universalize the meaning of the Quilt but also it establishes a monolithic viewing position from which to receive that meaning. Here, I outline an alternative model of reception which is implicit in the design and display of the Quilt. While this model acknowledges reverence as one potential response to the Quilt, it does not foreclose other ways of reading. Rather than facilitating a grave performance, then, the Quilt enables performances within the grave. These performances are constituted in/through a dynamic exchange between speaker and listener, text and context, and work to produce a range of ideological meanings and subject positions. To understand how the Quilt locates its viewers within a particular subject position, it is first necessary to specify the speaker-listener relationship established in displays of the Quilt. This relationship is played out through a series of confessional utterances which, as Michel Foucault explains in The History of Sexuality, Volume 1, imply a dialectic relationship between speaker and listener in which subjectivity is predicated on subjection (61-62). The speaker's desire to confess necessitates the presence of a listener, one not wholly passive since his/her presence incites and enables the will to confess. In this way, both speaker and listener are marked as active/passive agents in an exchange characterized by reciprocity and negotiation. Because speakers and listeners simultaneously serve as subject and object of the confession, the exchange cannot be represented as static (active/passive) or unidirectional (sender-message-receiver). Since many critics already have carefully delineated the processes through which the Quilt directs the address of the panels and constitutes the dead as subjects, here I want to focus on how spectators are constructed as subjects who bear witness. Critics typically posit viewers of the Quilt as unified, coherent, monolithic subjects; yet Foucault's discussion of the confessional exchange assumes a subject-in-process. For me, this process is most accurately characterized as schizophrenic. My use of schizophrenia is tropic rather than diagnostic, in that the term works figuratively to describe subject formation rather than to identify the nature of psychiatric disturbance. Two characteristics (which are derived from the symptomatology of the psychic disorder schizophrenia) define the schizophrenic spectator: one, the loss of "normal" associations; and two, the presence of "auditory hallucinations." The first characteristic of schizophrenic spectators is the loss of "normal" associations. Remi J. Cadoret notes that in schizophrenics, "[t]hought processes appear to lose their normal associations, or usual connecting links, so that the individual is often unable to focus his [sic] thinking upon a particular mental task" (481). For schizophrenics, conventional relational markers (such as chronology, causality, temporality, and spatiality) no longer order cognition; instead, these markers are distorted (if not entirely ineffectual), creating for the schizophrenic a fractured sense of self in/and world. Without these normal associations, the schizophrenic wanders aimlessly (and often in isolation) through a chaotic world in search of structure, meaning, and purpose. Temporal associations provide perhaps the most common means of ordering experience. Viewed as a linear progression characterized by movement, change, and renewal, time structures the historical and the everyday by sequencing, demarcating, and hierarchizing events. Within the Quilt, this sense of progression is supplanted by perpetual repetition of the present. Elsley has offered a similar observation, noting that the Quilt operates in a "transitory present" tense, it "exists in a continual state of becoming" (194). In one sense, this perpetual present tense derives from the fact that no two displays of the Quilt are identical. Panels are ordered differently, new names and panels are added, older panels begin to show signs of wear-and-tear. A perpetual present tense also derives from the fact that the Quilt charts the progression of an epidemic that is itself ongoing, incomplete. Thus, the landscape of the Quilt is re-mapped in light of advances in HIV-treatment, softening/tightening of social mores, and changes in AIDS demographics. Another common means of ordering experience is though spatial associations. Location orders the social through architecture, urban planning, and zoning, endowing spaces with a well-defined purpose and layout. Yet the Quilt provides few signals regarding how spectators are intended to navigate its surface. As Weinberg notes, the Quilt is a "great grid" with "no narrative, no start or finish" (37). By describing the Quilt as a "grid," Weinberg implicitly ascribes to the artifact a controlling logic, a unified design--what in quilting parlance is termed a patchwork sampler. This design pattern, however, does not direct the flow of spectators in a single stream of traffic. This is so because, unlike a Drunkard's Path or Double Wedding Band pattern in which the individual panel blocks work together to create a unified design across the surface of the quilt, a patchwork sampler is constituted by a series of single panel blocks, each with a unique design, history, and logic. As a result, patrons' movements are guided by associations and punctuated by pauses, interruptions, and abrupt changes in course. The randomness of engagement is further enabled by the muslin walkways which visually separate the panels, marking each as distinct and disallowing any sense of continuity (narrative, spatial) among them. The routes which visitors of the Quilt traverse thus are transitory and ephemeral, simultaneously charted and erased in the moment of passing by. The second characteristic of schizophrenic spectators is the presence of auditory hallucinations. Cadoret defines these hallucinations as "the perception of auditory stimuli, or sounds, where none are externally present . . . . The voices . . . may repeat his [sic] thoughts or actions, argue with him [sic], or threaten, scold, or cure him [sic]" (481). Auditory hallucinations can lead the schizophrenic to believe that s/he is under constant surveillance or can cause the schizophrenic to slip further into a self-contained, isolated world of delusion. That the Quilt is made up of "a myriad of individual voices" (Elsley 192) is immediately apparent in the number of individuals who have taken part in its construction and display. With each quilt panel, spectators are confronted with multiple voices--the person who has died, the person(s) who made the block, the person(s) who stitched the block to others for a specific display, and so on. Moreover, the Quilt places these "individual voices . . . in the context of community" (Elsley 191). For Quilt spectators, then, memories of a life lived coexist with grief over a life cut short, anger at institutional apathy and systemic homophobia, faith in the import of remembering those who have died, and so on. Each of these voices vie for the spectator's attention, facilitating a gaze that is dynamic, multidirectional, mobile. Because the gaze is not fixed, the Quilt cannot convey a solitary truth claim to its viewers; rather, spectators must immerse themselves within the delusion and confusion of voices, imposing some sense of order on their own viewing experiences. Given that persons with AIDS continue to be marginalized within American culture, my use of the schizophrenic spectator to trace the reception dynamic of Quilt exhibits might appear to perpetuate, rather than unsettle, dominant ideological formations. Of course, this is the critical conundrum at the center of all investigations into subject formation: that is, "how to take an oppositional relation to power that is, admittedly, implicated in the very power one opposes" (Butler 17). Despite the potential pitfalls, I nonetheless use the trope of schizophrenia precisely because it recognizes the ways in which Quilt spectators, persons with AIDS, and persons who have died of AIDS-related illnesses are divested of the power and authority to speak even before they begin speaking. Furthermore, because the schizophrenic subject is founded on ever-shifting affinities (in time, across space), the position enables spectators to chart alternative lines of relation among institutional practices, ideological formations, and individual experiences, thus potentially mobilizing and sustaining a shared political program. It is on these twin goals of pedagogy and polemics that the NAMES Project originally was founded, and it is to these goals that we now must return. References Butler, Judith. The Psychic Life of Power: Theories in Subjection. Stanford: Stanford UP, 1997. Cadoret, Remi J. "Schizophrenia." Collier's Encyclopedia. Vol. 20. Eds. Lauren S. Bahr, et. al. New York: Collier's, 1997. 480-482. Elsley, Judy. "The Rhetoric of the NAMES Project AIDS Quilt: Reading the Text(ile)." AIDS: The Literary Response. Ed. Emmanuel S. Nelson. New York: Twayne, 1992. 187-196. Foucault, Michel. The History of Sexuality, Volume 1: An Introduction. Trans. Robert Hurley. New York: Vintage, 1990. Gentry, Jerry. "The NAMES Project: A Catharsis of Grief." The Christian CENTURY 23 May 1989: 550-551. Hawkins, Peter S. "Naming Names: The Art of Memory and the NAMES Project AIDS Quilt." Critical Inquiry 19(Summer 1993): 752-779. Weinberg, Jonathan. "The Quilt: Activism and Remembrance." Art in America 80(Dec. 1992): 37, 39.

Introduction How do we measure intimacy? What are its impacts on our social, political and personal lives? Can we claim a politics to our intimate lives that escapes the normative confines of archaic institutions, while making social justice claims for relationship recognition? Negotiating some of these disparate questions requires us to think more broadly in contemporary public debates on equality and relationship recognition. Specifically, by outlining the impacts of the popular "gay marriage" debate, this paper examines the impacts of queer theory in association with public policy and community lobbying for relationship equality. Much of the debate remains polarised: eliminating discrimination is counterposed to religious or reproductive narratives that suggest such recognition undermines the value of the "natural" heterosexual family. Introducing queer theory into advocacy that oscillates between rights and reproduction problematises indexing intimacy against normative ideas of monogamy and family. While the arguments circulated by academics, lawyers, politicians and activists have disparate political and ethical impacts, when taken together, they continue to define marriage as a public regulation of intimacy and citizenship. Citizenship, measured in democratic participation and choice, however, can only be realised through reflexive politics that value difference. Encouraging critical dialogue across disparate areas of the marriage equality debate will have a significant impact on how we make ethical claims for recognising intimacy. (Re)defining Marriage In legislative terms, marriage remains the most fundamental means through which the relationship between citizenship and intimacy is crystallised in Australia. For example, in 2004 the Federal Liberal Government in Australia passed a legislative amendment to the Marriage Act 1961 and expressly defined marriage as a union between a man and a woman. By issuing a public legislative amendment, the Government intended to privilege monogamous (in this case understood as heterosexual) intimacy by precluding same-sex or polygamous marriage. Such an exercise had rhetorical rather than legal significance, as common law principles had previously defined the scope of marriage in gender specific terms for decades (Graycar and Millbank 41). Marriage as an institution, however, is not a universal or a-historical discourse limited to legal or political constructs. Socialist feminist critiques of marriage in the 1950s conceptualised the legal and gender specific constructs in marriage as a patriarchal contract designed to regulate female bodies (Hannam 146). However, Angela McRobbie notes that within a post-feminist context, these historical realities of gendered subjugation, reproduction or domesticity have been "disarticulated" (26). Marriage has become a more democratic and self-reflexive expression of intimacy for women. David Shumway elaborates this idea and argues that this shift has emerged in a context of "social solidarity" within a consumer environment of social fragmentation (23). What this implies is that marriage now evokes a range of cultural choices, consumer practices and affective trends that are incommensurable to a singular legal or historical term of reference. Debating the Politics of Intimacy and Citizenship In order to reflect on this shifting relationship between choice, citizenship and marriage as a concept, it is necessary to highlight that marriage extends beyond private articulations of love. It is a ritualised performance of heterosexual individual (or coupled) citizenship as it entrenches economic and civil rights and responsibilities. The private becomes public. Current neo-liberal approaches to same-sex marriage focus on these symbolic and economic questions of how recognising intimacy is tied to equality. In a legal and political context, marriage is defined in s5 Marriage Act as "the union between a man and a woman to the exclusion of all others, voluntarily entered into for life." While the Act does not imbue marriage with religious or procreative significance, such a gender dichotomous definition prevents same-sex and gender diverse partners from entering into marriage. For Morris Kaplan, this is a problem because "full equality for lesbian and gay citizens requires access to the legal and social recognition of our intimate associations" (201). Advocates and activists define the quest for equal citizenship by engaging with current religious dogma that situates marriage within a field of reproduction, whereby same-sex marriage is seen to rupture the traditional rubric of monogamous kinship and the biological processes of "gender complementarity" (Australian Christian Lobby 1). Liberal equality arguments reject such conservative assertions on the basis that desire, sexuality and intimacy are innate features of human existence and hence always already implicated in public spheres (Kaplan 202). Thus, legal visibility or state recognition becomes crucial to sustaining practices of intimacy. Problematising the broader social impact of a civil rights approach through the perspective of queer theory, the private/public distinctions that delineate citizenship and intimacy become more difficult to negotiate. Equality and queer theory arguments on same-sex marriage are difficult to reconcile, primarily because they signify the different psychic and cultural investments in the monogamous couple. Butler asserts that idealisations of the couple in legal discourse relates to norms surrounding community, family and nationhood (Undoing 116). This structured circulation of sexual norms reifies the hetero-normative forms of relationships that ought to be recognised (and are desired) by the state. Butler also interrogates this logic of marriage, as a heterosexual norm, and suggests it has the capacity to confine rather than liberate subjects (Undoing 118-20). The author's argument relies upon Michel Foucault's notion of power and subjection, where the subject is not an autonomous individual (as conceived in neo liberal discourses) but a site of disciplined discursive production (Trouble 63). Butler positions the heterosexuality of marriage as a "cultural and symbolic foundation" that renders forms of kinship, monogamy, parenting and community intelligible (Undoing 118). In this sense, marriage can be a problematic articulation of state interests, particularly in terms of perpetuating domesticity, economic mobility and the heterosexual family. As former Australian Prime Minister John Howard opines: Marriage is … one of the bedrock institutions of our society … marriage, as we understand it in our society, is about children … providing for the survival of the species. (qtd. in Wade) Howard's politicisation of marriage suggests that it remains crucial to the preservation of the nuclear family. In doing so, the statement also exemplifies homophobic anxieties towards non-normative kinship relations "outside the family". The Prime Ministers' words characterise marriage as a framework which privileges hegemonic ideas of monogamy, biological reproduction and gender dichotomy. Butler responds to these homophobic terms by alluding to the discursive function of a "heterosexual matrix" which codes and produces dichotomous sexes, genders and (hetero)sexual desires (Trouble 36). By refusing to accept the binary neo-liberal discourse in which one is either for or against gay marriage, Butler asserts that by prioritising marriage, the individual accepts the discursive terms of recognition and legitimacy in subjectifying what counts as love (Undoing 115). What this author's argument implies is that by recuperating marital norms, the individual is not liberated, but rather participates in the discursive "trap" and succumbs to the terms of a heterosexual matrix (Trouble 56). In contradistinction to Howard's political rhetoric, engaging with Foucault's broader theoretical work on sexuality and friendship can influence how we frame the possibilities of intimacy beyond parochial narratives of conjugal relationships. Foucault emphasises that countercultural intimacies rely on desires that are relegated to the margins of mainstream (hetero)sexual culture. For example, the transformational aesthetics in practices such as sadomasochism or queer polyamorous relationships exist due to certain prohibitions in respect to sex (Foucault, History (1) 38, and "Sex" 169). Foucault notes how forms of resistance that transgress mainstream norms produce new experiences of pleasure. Being "queer" (though Foucault does not use this word) becomes identified with new modes of living, rather than a static identity (Essential 138). Extending Foucault, Butler argues that positioning queer intimacies within a field of state recognition risks normalising relationships in terms of heterosexual norms whilst foreclosing the possibilities of new modes of affection. Jasbir Puar argues that queer subjects continue to feature on the peripheries of moral and legal citizenship when their practices of intimacy fail to conform to the socio-political dyadic ideal of matrimony, fidelity and reproduction (22-28). Puar and Butler's reluctance to embrace marriage becomes clearer through an examination of the obiter dicta in the recent American jurisprudence where the proscription on same-sex marriage was overturned in California: To the extent proponents seek to encourage a norm that sexual activity occur within marriage to ensure that reproduction occur within stable households, Proposition 8 discourages that norm because it requires some sexual activity and child-bearing and child-rearing to occur outside marriage. (Perry vs Schwarzenegger 128) By connecting the discourse of matrimony and sex with citizenship, the court reifies the value of marriage as an institution of the family, which should be extended to same-sex couples. Therefore, by locating the family in reproductive heterosexual terms, the court forecloses other modes of recognition or rights for those who are in non-monogamous relationships or choose not to reproduce. The legal reasoning in the case evinces the ways in which intimate citizenship or legitimate kinship is understood in highly parochial terms. As Kane Race elaborates, the suturing of domesticity and nationhood, with the rhetoric that "reproduction occur within stable households", frames heterosexual nuclear bonds as the means to legitimate sexual relations (98). By privileging a familial kinship aesthetic to marriage, the state implicitly disregards recognising the value of intimacy in non-nuclear communities or families (Race 100). Australia, however, unlike most foreign nations, has a dual model of relationship recognition. De facto relationships are virtually indistinguishable from marriage in terms of the rights and entitlements couples are able to access. Very recently, the amendments made by the Same-Sex Relationships (Equal Treatment in Commonwealth Laws - General Reform) Act 2008 (Cth) has ensured same-sex couples have been included under Federal definitions of de facto relationships, thereby granting same-sex couples the same material rights and entitlements as heterosexual married couples. While comprehensive de facto recognition operates uniquely in Australia, it is still necessary to question the impact of jurisprudence that considers only marriage provides the legitimate structure for raising children. As Laurent Berlant suggests, those who seek alternative "love plots" are denied the legal and cultural spaces to realise them ("Love" 479). Berlant's critique emphasises how current "progressive" legal approaches to same-sex relationships rely on a monogamous (heterosexual) trajectory of the "love plot" which marginalises those who are in divorced, single, polyamorous or multi-parent situations. For example, in the National Year of Action, a series of marriage equality rallies held across Australia over 2010, non-conjugal forms of intimacy were inadvertently sidelined in order to make a claim for relationship recognition. In a letter to the Sydney Star Observer, a reader laments: As a gay man, I cannot understand why gay people would want to engage in a heterosexual ritual called marriage … Why do gay couples want to buy into this ridiculous notion is beyond belief. The laws need to be changed so that gays are treated equal under the law, but this is not to be confused with marriage as these are two separate issues... (Michael 2) Marriage marks a privileged position of citizenship and consumption, to which all other gay and lesbian rights claims are tangential. Moreover, as this letter to the Sydney Star Observer implies, by claiming sexual citizenship through the rubric of marriage, discussions about other campaigns for legislative equality are effectively foreclosed. Melissa Gregg expands on such a problematic, noting that the legal responses to equality reiterate a normative relationship between sexuality and power, where only couples that subscribe to dyadic, marriage-like relationships are offered entitlements by the state (4). Correspondingly, much of the public activism around marriage equality in Australia seeks to achieve its impact for equality (reforming the Marriage Act) by positioning intimacy in terms of state legitimacy. Butler and Warner argue that when speaking of legitimacy a relation to what is legitimate is implied. Lisa Bower corroborates this, asserting "legal discourse creates norms which universalise particular modes of living…while suppressing other practices and identities" (267). What Butler's and Bower's arguments reveal is that legitimacy is obtained through the extension of marriage to homosexual couples. For example, Andrew Barr, the current Labor Party Education Minister in the Australian Capital Territory (ACT), noted that "saying no to civil unions is to say that some relationships are more legitimate than others" (quoted in "Legal Ceremonies"). Ironically, such a statement privileges civil unions by rendering them as the normative basis on which to grant legal recognition. Elizabeth Povinelli argues the performance of dyadic intimacy becomes the means to assert legal and social sovereignty (112). Therefore, as Jenni Millbank warns, marriage, or even distinctive forms of civil unions, if taken alone, can entrench inequalities for those who choose not to participate in these forms of recognition (8). Grassroots mobilisation and political lobbying strategies around marriage equality activism can have the unintentional impact, however, of obscuring peripheral forms of intimacy and subsequently repudiating those who contest the movement towards marriage. Warner argues that those who choose to marry derive pride from their monogamous commitment and "family" oriented practice, a privilege afforded through marital citizenship (82). Conversely, individuals and couples who deviate from the "normal" (read: socially palatable) intimate citizen, such as promiscuous or polyamorous subjects, are rendered shameful or pitiful. This political discourse illustrates that there is a strong impetus in the marriage equality movement to legitimate "homosexual love" because it mimics the norms of monogamy, stability, continuity and family by only seeking to substitute the sex of the "other" partner. Thus, civil rights discourse maintains the privileged political economy of marriage as it involves reproduction (even if it is not biological), mainstream social roles and monogamous sex. By defining social membership and future life in terms of a heterosexual life-narrative, same-sex couples become wedded to the idea of matrimony as the basis for sustainable intimacy and citizenship (Berlant and Warner 557). Warner is critical of recuperating discourses that privilege marriage as the ideal form of intimacy. This is particularly concerning when diverse erotic and intimate communities, which are irreducible to normative forms of citizenship, are subject to erasure. Que(e)rying the Future of Ethics and Politics By connecting liberal equality arguments with Butler and Warner's work on queer ethics, there is hesitation towards privileging marriage as the ultimate form of intimacy. Moreover, Butler stresses the importance of a transformative practice of queer intimacy: It is crucial…that we maintain a critical and transformative relation to the norms that govern what will not count as intelligible and recognisable alliance and kinship. (Undoing 117) Here the author attempts to negotiate the complex terrain of queer citizenship and ethics. On one hand, it is necessary to be made visible in order to engage in political activism and be afforded rights within a state discourse. Simultaneously, on the other hand, there is a need to transform the prevailing hetero-normative rhetoric of romantic love in order to prevent pathologising bodies or rendering certain forms of intimacy as aberrant or deviant because, as Warner notes, they do not conform to our perception of what we understand to be normal or morally desirable. Foucault's work on the aesthetics of the self offers a possible transformational practice which avoids the risks Warner and Butler mention because it eludes the "normative determinations" of moralities and publics, whilst engaging in an "ethical stylization" (qtd. in Race 144). Whilst Foucault's work does not explicitly address the question of marriage, his work on friendship gestures to the significance of affective bonds. Queer kinship has the potential to produce new ethics, where bodies do not become subjects of desires, but rather act as agents of pleasure. Negotiating the intersection between active citizenship and transformative intimacy requires rethinking the politics of recognition and normalisation. Warner is quite ambivalent as to the potential of appropriating marriage for gays and lesbians, despite the historical dynamism of marriage. Rather than acting as a progressive mechanism for rights, it is an institution that operates by refusing to recognise other relations (Warner 129). However, as Alexander Duttmann notes, recognition is more complex and a paradoxical means of relation and identification. It involves a process in which the majority neutralises the difference of the (minority) Other in order to assimilate it (27). However, in the process of recognition, the Other which is validated, then transforms the position of the majority, by altering the terms by which recognition is granted. Marriage no longer simply confers recognition for heterosexual couples to engage in reproduction (Secomb 133). While some queer couples may subscribe to a monogamous relationship structure, these relationships necessarily trouble conservative politics. The lamentations of the Australian Christian Lobby regarding the "fundamental (anatomical) gender complementarity" of same-sex marriage reflect this by recognising the broader social transformation that will occur (and already does with many heterosexual marriages) by displacing the association between marriage, procreation and parenting (5). Correspondingly, Foucault's work assists in broadening the debate on relationship recognition by transforming our understanding of choice and ethics in terms of "queer friendship." He describes it as a practice that resists the normative public distinction between romantic and platonic affection and produces new aesthetics for sexual and non-sexual intimacy (Foucault, Essential 170). Linnell Secomb argues that this "double potential" alluded to in Foucault and Duttman's work, has the capacity to neutralise difference as Warner fears (133). However, it can also transform dominant narratives of sexual citizenship, as enabling marriage equality will impact on how we imagine traditional heterosexual or patriarchal "plots" to intimacy (Berlant, "Intimacy" 286). Conclusion Making an informed impact into public debates on marriage equality requires charting the locus of sexuality, intimacy and citizenship. Negotiating academic discourses, social and community activism, with broader institutions and norms presents political and social challenges when thinking about the sorts of intimacy that should be recognised by the state. The civil right to marriage, irrespective of the sex or gender of one's partner, reflects a crucial shift towards important democratic participation of non-heterosexual citizens. However, it is important to note that the value of such intimacy cannot be indexed against a single measure of legal reform. While Butler and Warner present considered indictments on the normalisation of queer intimacy through marriage, such arguments do not account for the impacts of que(e)rying cultural norms and practices through social and political change. Marriage is not a singular or a-historical construction reducible to state recognition. Moreover, in a secular democracy, marriage should be one of many forms of diverse relationship recognition open to same-sex and gender diverse couples. In order to expand the impact of social and legal claims for recognition, it is productive to rethink the complex nature of recognition, ritual and aesthetics within marriage. In doing so, we can begin to transform the possibilities for articulating intimate citizenship in plural democracies. References Australian Christian Lobby. "Submission to the Senate Legal and Constitutional Affairs Legislation Committee Inquiry into the Marriage Equality Amendment Bill 2009." Deakin: ACL, 2009. Australian Government. "Sec. 5." Marriage Act of 1961 (Cth). 1961. ———. Same-Sex Relationships (Equal Treatment in Commonwealth Laws - General Reform) Act 2008 (Cth). 2008. Bell, David, and John Binnie. The Sexual Citizen: Queer Politics and Beyond. Oxford: Polity P, 2000. Berlant, Lauren. "Intimacy: A Special Issue." Critical Inquiry 24 (1998): 281-88. ———. "Love, a Queer Feeling." Homosexuality and Psychoanalysis. Eds. Tim Dean and Christopher Lane. Chicago: U of Chicago P, 2001:432-52. Berlant, Lauren, and Michael Warner. "Sex in Public." Ed. Lauren Berlant. Intimacy. Chicago and London: U of Chicago P, 2000: 311-30. Bower, Lisa. "Queer Problems/Straight Solutions: The Limits of a Politics of 'Official Recognition'" Playing with Fire: Queer Politics, Queer Theories. Ed. Shane Phelan. London and New York: Routledge, 1997: 267-91. Butler, Judith. Gender Trouble: Feminism and the Subversion of Identity. New York and London: Routledge, 1990. ———. Undoing Gender. New York: Routledge, 2004. Duttmann, Alexander. Between Cultures: Tensions in the Struggle for Recognition. London: Verso, 2000. Foucault, Michel. The History of Sexuality (1): The Will to Knowledge. London: Penguin Books, 1977. ———. "Sex, Power and the Politics of Identity." Ethics: Subjectivity and Truth. Ed. Paul Rabinow. London: Allen Lange/Penguin, 1984. 163-74. ———. Essential Works of Foucault: 1954-1984: Ethics, Vol. 1. London: Penguin, 2000. Graycar, Reg, and Jenni Millbank. "From Functional Families to Spinster Sisters: Australia's Distinctive Path to Relationship Recognition." Journal of Law and Policy 24. 2007: 1-44. Gregg, Melissa. "Normal Homes." M/C Journal 10.4 (2007). 27 Aug. 2007 ‹http://journal.media-culture.org.au/0708/02-gregg.php›. Hannam, Jane. Feminism. London and New York: Pearson Education, 2007. Kaplan, Morris. "Intimacy and Equality: The Question of Lesbian and Gay Marriage." Playing with Fire: Queer Politics, Queer Theories. Ed. Shane Phelan. London and New York: Routledge, 1997: 201-30. "Legal Ceremonies for Same-Sex Couples." ABC Online 11 Nov. 2009. 13 Dec. 2011 ‹http://www.abc.net.au/news/stories/2009/11/11/2739661.htm›. McRobbie, Angela. The Aftermath of Feminism: Gender, Culture and Social Change. London and New York: Sage, 2008. Michael. "Why Marriage?" Letter to the Editor. Sydney Star Observer 1031 (20 July 2010): 2. Millbank, Jenni. "Recognition of Lesbian and Gay Families in Australian Law - Part One: Couples." Federal Law Review 34 (2008): 1-44. Perry v. Schwarzenegger. 3: 09 CV 02292. United States District Court for the Northern District of California. 2010. Povinelli, Elizabeth. Empire of Love: Toward a Theory of Intimacy, Genealogy and Carnality. Durham: Duke UP, 2006. Puar, Jasbir. Terrorist Assemblages: Homonationalism in Queer Times. Durham: Duke UP, 2007. Race, Kane. Pleasure Consuming Medicine: The Queer Politics of Drugs. Durham and London: Duke UP, 2009. Secomb, Linnell. Philosophy and Love. Edinburgh: Edinburgh UP, 2007. Shumway, David. Modern Love: Romance, Intimacy and the Marriage Crisis. New York: New York UP, 2003. Wade, Matt. "PM Joins Opposition against Gay Marriage as Cleric's Election Stalls." The Sydney Morning Herald 6 Aug. 2003. Warner, Michael. The Trouble with Normal: Sex, Politics and the Ethics of Queer Life. Cambridge: Harvard UP, 1999.

Preamble: Medium/Format/Marker Medium/Format/Marker (M/F/M) was a visual-aural improvisational performance involving myself, and musicians Joe Sorbara, and Ben Grossman. It was formed through my work as a PhD candidate at the Improvisation, Community, and Social Practice research initiative at the University of Guelph. This performance was conceived as an attempted intervention against the propensity to reify the “new.” It also sought to address the proliferation of the screen and question how the increased presence of screens in everyday life has augmented the way in which an audience is conceived and positioned. This conception is in direct conversation with my thesis, which is a practice-based research project exploring what the experimental combination of intermediality, improvisation, and the cinema might offer towards developing a reflexive approach to "new" media, screen culture, and expanded cinemas. One of the ways I chose to explore this area involved developing an interface that allowed an audio-visual ensemble to improvise with a film's audio-visual projection. I experimented with different VJ programs. These programs often utilize digital filters and effects to alter images through real-time mixing and layering, much like a DJ does with sound. I found a program developed by Chicago-based artist Ontologist called Ontoplayer, which he developed out of his practice as an improvisational video artist. The program works through a dual-channel interface where two separate digital files could be augmented, with their projected tempo capable of being determined by musicians through a MIDI interface. I conceptualized the performance around the possibility of networking myself with two other musicians via this interface. I approached percussionist Joe Sorbara and multi-instrumentalist Ben Grossman with the idea to use Ontoplayer as a means to improvise with Chris Marker's La Jetée (1962, 28 mins). The film itself would be projected simultaneously in four different formats: 16mm celluloid, VHS, Blu-ray, and Standard Definition video (the format the ensemble improvised with) projected onto four separate screens. From left to right, the first screen contained the projected version of La Jetée that we improvised with, next to it was its Blu-ray format, next to that, a degraded VHS copy of the film, and next to that, the 16mm print. The performance materialized through performing a number of improvisatory experiments. A last minute experiment conceived a few hours before the performance involved placing contact microphones overtop of the motor on a Bell & Howell 16mm projector. The projector was tested in the days leading up to the performance and it ran as smoothly as could be expected. It had a nice cacophonous hum that Ben Grossman intended to improvise with using some contact mics attached directly over the projector’s motor, a $5 iPad app, and his hurdy-gurdy. Fifteen minutes before the performance began, the three of us huddled to discuss how long we'd like to go. We had met briefly the day before to discuss the technical setup of the performance but not its execution and length. I hadn't considered duration. Joe broke the silence by asking if we'd be "finding beginnings and endings." I didn't know what that entailed, but nodded. We started. I turned on the projector and it immediately started to cough and chew on the 40 year old 16mm print I found online. My first impulse was to intervene, to try to save it. The film continued and I sat frozen for a moment. Joe started playing and Ben, expecting me to send him the audio track from La Jetée, prompted me to do so. I let the projector go and began. Joe had a digital kick-drum and two contact mics on his drum kit hooked into a MIDI hub, while Ben's hurdy-gurdy had a contact mic inside it, wired into the hub. The hub hooked into my laptop and allowed for an intermedial conversation to emerge between the three of us. While the 16mm, VHS, and Blu-Ray formats proceeded relatively unimpeded alongside each other on their respective screens, the fourth screen was where this conversation took place. I digitally reordered different image sequences from La Jetée. The fact that it’s a film (almost) comprised entirely of still images made this reordering intriguing in that I was able control the speed of progressing from each image to the next. The movement from image to image was structured between Ben and Joe’s improvisations and the kind of effects and filters I had initialized. Ontoplayer has a number of effects and filters that push the base image into more abstract territories (e.g.: geometric shapes, over pixelation) I was uninterested in exploring. I utilized effects that to some degree still kept the representational content of the image intact. The degree to which these effects took hold of the image were determined by whether or not Ben and Joe decided to use the part of their instrument that would trigger them. The decision to linger on an image, colour it differently, or skip ahead in the film’s real-time projection destabilized my sense of where I was in the film. It became an event in the sense that each movement, both visual and aural was happening with an indeterminate duration. La Jetée opens with the narrator proclaiming: “this is the story of a man marked by an image from his childhood.” The story itself is situated around a man in a post-apocalyptic world, haunted by the persistent memory of a woman he saw as a child while standing on the jetty at Orly Airport in Paris. The man was a soldier, now captured, and imprisoned in an underground camp. The prison guards have been conducting experiments on the prisoners, attempting to use the prisoner’s memories as a mechanism to send them backwards and forwards in time. The narrator explains, “with the surface of the planet irradiated … The human race was doomed. Space was off limits. The only link with survival passed through time … The purpose of the experiments was to throw emissaries into time to call the past and future to the aid of the present.” La Jetée is visually structured as a photomontage, with voice-over narration, diegetic and non-diegetic sound existing as component parts to the whole film. I decided to separate these components for the sake of isolating them before the performance as instruments of the film to be improvisationally deployed through the intermedial connection between Ben, Joe, and myself. The resulting projections that emerged from our interface became a kind of improvised "grooving" to La Jetée that restricted the impulse to discriminately place sound beneath and behind the image. I selected images from different points in the film that felt "timely" given the changing dynamic between the three of us. I remember lingering on an image of the woman's face, her hand against her mouth, her hair being blown back by the wind. I looked and listened for the moment when the film would catch and then catch fire. It never came. We let the reel run to the end and continued on improvising until we found an ending. But the sound of that film catching but never breaking, the intention and tension of the film being near death the entire time made everything we did more precious, teetering on the brink of failure. We could never have predicted that, and it gave us something I continue to ponder and be thankful for. Celluloid junkies in the room commented on how precipitous the whole thing was, given how rare it is to encounter the sound of celluloid film travelling through a projector inside a cinematic space. An audiophile mused over how there wasn’t any document, his mind adequately blown by how “funky” the projector sounded. With there being no document of the performance, I'm left with my own memories. In mining the aftermath of this performance, I hope to find an addendum that considers how improvisation might negotiate with augmentation in ways that speak to Walter Benjamin's assertion that the "camera, the film, on the one hand, extends our comprehension of the necessities which rule our lives; on the other hand, it manages to assure us of an immense and unexpected field of action” (Benjamin 236-7).Images to be Determined I got a job working in a photo lab eight years ago, right around the time digital cameras started becoming not only affordable, but technologically-comparable alternatives to film cameras. The photo printer in the lab was setup to scan and digitize celluloid filmstrips to allow for digital “touchups” by the technician. It was also hooked into touchscreen media stations that accepted a variety of memory card formats so that customers could “touchup” their own images. Celluloid film meant that as long as their format was chemical, touching up their images remained the task of the technician. Against the urging of the lab’s manager, I resisted altering other people’s images. It felt like a violation, despite the fact that almost every customer was unaware of this process. They assumed a degree of responsibility for a chemically-exposed image. I still got blamed for a lot of bad photography, but an image chemically under or overexposed was irreparable. Digital cameras changed all of that. I still preferred an evenly exposed celluloid print to a digital, but the allure was the ability for these images to be augmented. Augmentation is synonymous with "enhancement," "prosthesis," "addition," "amplification," "enrichment," "expansion,” and "extension" (to name a few). For the purpose of this essay, I am situating augmentation as an agential act engaging with a static form to purposefully alter its aesthetic and political relation to a reality. To what extent can we say that the digital image is itself, an augmentation? If Instagram is any indication, the digital image's existence is bound by its perpetual augmentation. A digital image is only as good as its capacity to be worked on. The ubiquity of digitally applying lomographic filters to digital images, as a defining step in their distributive chain, is indicative of the discursive impact remediating the old into the new has on digital forms. These digitally-coded filters used to augment “clear” digital images are comprised of exaggerated imperfections that existed to varying degrees, as unforeseen side effects of working with comparatively more unstable celluloid textures. The filtered images themselves are digital distortions of a digital original. The filters augment this original through obscuring one or a number of components. Some filters might exaggerate the green values or sharpen a particular quadrant within the frame that might coincide with the look of a particular film stock from the past. The discourse of “film” and “vintage” photography has become a synonymous component of the digital aesthetic, discursively warming up what is often considered to be a cold, and disembodied medium. Augmentation works to re-establish a congruous relationship between the filmic and the digital, attempting to reconcile the aesthetic distance between granularity and pixelation. This is ironic because this process is encapsulated through digitally encoding and applying these filters for the sake of obscuring clarity. Thus, the object is both hailed as clear and clearly manipulable. Another example a bit closer to the cinema is the development of digital video cameras offering RAW, or minimally compressed file formats for the sole purpose of augmenting the initial recording in post-production workflows in an attempt to minimize degradation in the image. The colour values and dynamic range of these images are muted, or flattened so that the human can control their elevation after the fact. To some degree the initial image, in itself, is an augmentation of its filmic relative. From early experiments with video synthesizers to the present digital coding of film effects, digital images have tantalized video artists and filmmakers with possibility shrouded in instantaneity and malleability. A key problem with this structure remains the unbridled proliferation and expansion of the digital image, set free for the sake of newness. How might improvisation work towards establishing an ethics of augmentation? An ethics of this kind must disrupt the popular notion of the digital image existing beyond analogical constraints. The belief that “if you can imagine it, you can do it” obfuscates the reality that to work with images, whatever their texture, is a negotiation with constraint. Part of M/F/M’s fruition emerged from a conversation I'd had with Canadian Animator Pierre Hébert last summer. Now obvious, but for Hébert, the first obstacle he needed to overcome as an improviser was developing an instrument that he could gig with. Through the act of designing an instrument I immediately became aware of what wasn't possible, and so the work leading up to the performance involved attempting to expand the possibilities of that instrument. How might I conceive of my own treatment of images simultaneously treated by Joe and Ben as a kind of cinematic extended technique we collaboratively bring into being? Constraint necessitates the need for extension, for finding new ways to sound and appear. Constraint is also consistently conceived as shackling progress. In scientific methodologies it is often arbitrarily imposed to steer an experiment into a desired direction. This sort of experimental methodology is in the business of presupposing outcomes, which I feel is often the case with what ultimately becomes the essay of end result in Humanities research. Constraint is an important imposition in improvisation only if the parties involved are willing to find new ways to move in consort with it. The act of improvisation is thus an engagement with the spatio-temporal constraints of performance, politics, memory, texture, and difference. My conception of the cinema is that of an instrument, whose past is what I work with to better understand its future. Critic Gene Youngblood, in his landmark book, Expanded Cinema, theorized a new conception of the cinema as a global planetary phenomenon suffused inside a space of intermedia, where immersive, interactive, and interconnected realms necessitated the need to critically conceptualise the cinema in cosmic terms. At around the time of Youngblood's writing, another practitioner of the cosmic way, improviser and composer Sun Ra was staking a similar claim for music's ability to uplift the species cosmically. Ra's popular line “If we came from nowhere here, why can’t we go somewhere there?” (Heble 125), articulated the problematic racial politics in post-WWII America, that fixed African-American identity into a static domain with little room to move upward. The "somewhere there" to Ra was a non-space, created from "a desire to opt out of the very codes of representation and intelligibility, the very frameworks of interpretation and assumption which have legitimated the workings of dominant culture" (Heble 125). Though Youngblood's and Ra's intellectual and creative impulses formed from differing political circumstances, the work and thinking of these two figures remain significant articulations of the need to work from and towards the cosmic. In 2003, Youngblood published a follow-up essay in a reprint of Expanded Cinema entitled Cinema and the Code. In it, he defines cinema as a “phenomenology of the moving image.” Rather than conceiving of it through any of its particular media, Youngblood advocates for a segregated conception of the cinema: Just as we separate music from its instruments. Cinema is the art of organizing a stream of audiovisual events in time. It is an event-stream, like music. There are at least four media through which we can practice cinema – film, video, holography, and structured digital code—just as there are many instruments through which we can practice music. (Youngblood cited in Marchessault and Lord 7) Music and cinema are thus conceived as the exterior consequences of creative and co-creative instrumental experimentation. For Ra and Youngblood, the planetary stakes of this project are infused with the need to manufacture and occupy an imaginative space (if only for a moment) outside of the known. This is not to say that the action itself is transcendental. But rather this outside is the planetary. For the past year I've been making a documentary with Joe Sorbara on the free improv scene in Toronto. Listening to musicians talk about improvisation in expansive terms, as this ethereal and ephemeral experience, that exists on the brink of failure, that is as much an act of memory as renewal, reverberated with my own feelings surrounding the cinema. Improvisation, to philosopher Gary Peters, is the "entwinement of preservation and destruction", that "invites us to make a transition from a closed conception of the past to one that re-thinks it as an endlessly ongoing event or occurrence whereby tradition is re-originated (Benjamin) or re-opened (Heidegger)” (Peters 2). This “entwinement of preservation and destruction” takes me back to my earlier discussion of the ways in which digital photography, in particular lomographically filtered snapshots, is structured through preserving the discursive past of film while destroying its standard. The performance of M/F/M attempted to connect the augmentation of the digital image and the impact this augmentation had on conceptualizing the past through an improvisational approach to intermediality. The issue I have with the determination of images concerns their technological standardization. As long as manufacturers and technicians control this process then the practice of gathering, projecting, and experiencing digital images is predetermined by their commercial obligation. It assures that augmenting the “immense and unexpected field of action” comprising the domain of images is itself a predetermination. References Benjamin, Walter. Illuminations. New York: Schocken Books, 1985. Heble, Ajay. Landing on the Wrong Note. London: Routledge, 2000. Marker, Chris, dir. La Jetée. Argos Films. 1962. Marchessault, Janine, and Susan Lord. Fluid Screens, Expanded Cinema. Toronto: University of Toronto Press, 2007. Peters, Gary. The Philosophy of Improvisation. Chicago: University of Chicago Press, 2009.