Дисертації з теми "ST14"
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Henry, Cindy. "Le rôle de ST18 dans la cellule pancréatique bêta." Thesis, Université Laval, 2011. http://www.theses.ulaval.ca/2011/28661/28661.pdf.
Повний текст джерелаZaya, Johan, and Amanda Strömberg. "Optimering av St1s anläggning med integrering : Vägen till en hållbar utveckling." Thesis, Högskolan i Borås, Akademin för textil, teknik och ekonomi, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-10278.
Повний текст джерелаMcElroy, Cameron Shea. "The Role of SULT2 ST1 in Zebrafish Development." University of Toledo / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1273786802.
Повний текст джерелаLarsson, Kim, and Emil Karlsson. "St1 Refinery - Biotreater : Optimering och utvecklingsmöjligheter med hänseende till miljön." Thesis, Högskolan i Borås, Akademin för textil, teknik och ekonomi, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-14976.
Повний текст джерелаBengtson, Mário Henrique. "Caracterização celular e molecular dos efeitos do ácido retinóico sobre as células ST1 de glioma de rato." Universidade de São Paulo, 2002. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-05072018-121057/.
Повний текст джерелаGliomas are the most fatal central nervous system tumors, for which efficient treatment is still not available. To analyze the cellular and molecular bases for the action of the differentiating and anti-tumor agent retinoic acid (ATRA) in gliomas, the rat glioma STl cell line was used as a model. We proposed: a) to analyze the effects of ATRA in STl cells morphology, growth and apoptosis; b) to isolate, identify and characterize the ATRA-induced genes in STl cells. We demonstrated that ATRA promotes cellular flattening and inhibition of DNA synthesis and growth in agarose suspension, characterizing a complete tumoral to normal phenotypic reversion, which is not accompanied by apoptosis. Subtracted cDNA libraries were generated, using 2 different methodologies: RDA (Representational Difference Analysis) and SSH (Suppressive Subtractive Hybridization) followed by macroarray screening. This allowed identification of 10 ATRA induced genes which are up regulated by ATRA during STl cells phenotypic reversion, namely: p450rai2, spi3, vegf, cdv-3a, okl38, eya2, gem, retSDR1, aldose redutase-like and a new gene with 61 % identity with chicken phosphatase. This study characterized the cellular and molecular effects of ATRA upon STl cells and allowed identification of new targets for future development of new drugs and gene therapy.
Tajjiou, Morad [Verfasser]. "Design und Entwicklung molekularbiologischer Modelle zur Charakterisierung der Mobilität des Clostridium difficile IStrons Cd/St1 / Morad Tajjiou." Mainz : Universitätsbibliothek der Johannes Gutenberg-Universität Mainz, 2021. http://d-nb.info/1238223796/34.
Повний текст джерелаVandewalle-Capo, Marine. "Étude de la sensibilité aux antibiotiques et aux peptides antimicrobiens humains de Legionella pneumophila." Thesis, Lyon, 2016. http://www.theses.fr/2016LYSE1291/document.
Повний текст джерелаLegionella pneumophila (Lp) is an accidental human pathogen which can infect alveolar macrophages and pneumocytes. During infection, Legionella have to deal with to various types of antibacterial agents, such as antimicrobial peptides (AMPs) produced by the host, and antibiotics with intracellular activity administered to patients. The mechanism of action of human AMPs against Legionella, and the resistance level to antibiotics of the bacterium are still poorly described. Our work aimed to contribute to a better understanding of the anti-Legionella activity of these molecules. The first part of this study consisted in the evaluation of the susceptibility of clinical Lp sg1 isolates to 8 antibiotics, to determine the epidemiological cut-off values of these different molecules. We demonstrated that all clinical isolates are susceptible to the tested antibiotics. The results revealed the presence of a subpopulation displaying a reduced susceptibility to macrolides. The analysis of the genomes allowed us to correlate this reduced susceptibility to le presence of the LpeAB macrolides efflux pump, found specifically in the sequence types ST1, ST701 and ST1335.The second part of this study was dedicated to the characterization of the antibacterial activity of the human AMPs LL-37 and HBD-3, and to the identification of their mechanism(s) of action against Legionella. All of the experiments show that LL-37 and HBD-3 induce a loss of cultivability by different mode of action. The results suggest that LL-37 is able to permeabilize the membrane of the L. pneumophila cells. Our findings also show that both peptides inhibit the intracellular replication of L. pneumophila, in part through collaboration with the host cell
Ortis, Fernanda. "Papel de BRG1 e Brm, reguladores globais de transcrição, na reversão fenotípica de células ST1 pela ação de glicocorticóides." Universidade de São Paulo, 2002. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-26072007-053713/.
Повний текст джерелаGlucocorticoid hormones (GCs) have been used as anti-inflammatory and anti-tumor agents, acting via nuclear receptors (GR) and being dependent on remodeling of the chromatin structure. As components of the global chromatin remodeling transcription complex (SWI/SNF), Brm and BRG-1 proteins play a key role in the action of GR. In order to study the mechanisms of action of GCs, we have been using the ST1 and P7 cell lines, derived from the C6, a rat glioma cell line. P7 is insensitive to the GC treatment, while ST1 displays a complete phenotypic reversion from tumoral to normal, including a G1-specific block in the cell cycle. A Brm and BRG1-specific polyclonal antiserum was generated, in rabbit, using recombinant hBRG1 protein as antigen. This antiserum was used to analyze the levels of Brm and BRG1 in these two cell lines, under GC treatment. While Brm is induced by GC, in ST1 cells, the basal level of Brm, in P7 cells, is relatively high, remaining unchanged under GC treatment. The possibility of brm mutations occurring in the P7 cells, was analyzed by DNA sequencing. Overexpression of brm and BRG1 in P7 cells led to morphological alterations (cell flattening) and decreased colony formation in agarose suspension and in solid substrate. Some of these clones became partially responsive to GC, when compared to the ST1 cell line. Co-immunoprecipitation assays revealed some differences in the SWI/SNF complex between ST1 and P7 cells.
Yang, Eric Vincent. "Modulation of the extracellular matrix proteins ST1 and MT2 during limb regeneration in two urodele amphibians, the newt, Notophthalmus viridescens, and the axolotl, Ambystoma mexicanum /." The Ohio State University, 1992. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487841548268774.
Повний текст джерелаCheung, Ka-chun. "Role of matriptase (ST14) in chronic myeloid leukaemia." Thesis, 2014. http://hdl.handle.net/2440/85926.
Повний текст джерелаThesis (Ph.D.) -- University of Adelaide, School of Medicine, 2014
(8037416), Anna C. Ratliff. "STRUCTURAL ANALYSIS AND CONFORMATIONAL DYNAMICS OF THE YEAST ISOPRENYLCYSTEINE CARBOXYL METHYLTRANSFERASE, STE14." Thesis, 2019.
Знайти повний текст джерелаCaaX proteins are involved in many key cellular processes such as proliferation, differentiation, trafficking, and gene expression. CaaX proteins have four specific C-terminal amino acids designated as a CaaX motif, where the “C” is a cysteine, “a” are aliphatic residues, and “X” represents one of several amino acids. Proteins with this motif undergo three post-translational modifications: isoprenylation of the cysteine residue, endoproteolysis of the –aaX residues and methylation of the isoprenylated cysteine, which is necessary for their localization in the cell and function. Due to involvement of CaaX proteins in many critical signaling pathways, mutations in CaaX proteins can result in a wide variety of disorders and carcinomas. Most notably, mutants in the KRAS gene are associated with 90% of pancreatic cancers and 30% of all cancers. Isoprenylcysteine carboxyl methyltransferase (Icmt), an integral membrane protein in the endoplasmic reticulum, is the only known protein responsible for the post-translational α-carboxyl methylesterification of the C-terminus of CaaX proteins. Cells with Icmt deficiency causes the small G-protein, K-ras, to be mislocalized and decreases downstream signaling of K-ras. Thus, our goal is to better understand the structure and methylation mechanism of Icmt in order to inhibit mutant K-ras in oncogenic cells and aid in the creation of a chemotherapeutic for pancreatic cancer.
Icmt studies have focused on the founding member of the Icmt family, Ste14. Ste14 is expressed in Saccharomyces cerevisiae (S. cerevisiae) and shares high homology with the human Icmt (hIcmt), which has yet to be functionally purified. Specifically, hIcmt and Ste14 share 63% similarity and 41% identity, mostly within the C-termini of the proteins. First, we optimized expression and purification of Ste14 in order to generate a larger yield of protein, which is necessary for many biophysical techniques. Infection of Sf9 cells with a baculovirus expressing an N-terminally 10-His-tagged and 3-myc-tagged Ste14 (His-Ste14), increased protein expression between four and five-fold compared to our yeast model and used significantly less starting materials. We also performed a detergent screen for the purification of His-Ste14 from insect cell expression. We concluded that n-Dodecyl-β-D-maltopyranoside (DDM), lauryl maltose neopentyl glycol (LMNG), and heptaethylene glycol monododecyl ether (C12E7) were detergents that stabilize His-Ste14 for further biophysical techniques. Additionally, we found 1xEQ buffer at pH 6.0 resulted in the most homogenous His-Ste14 sample.
Second, we sought to elucidate the SAM binding/ SAH release mechanism of His-Ste14 by utilizing a combinatorial method of site-directed spin labeling and electron paramagnetic resonance (EPR) spectroscopy analysis. We used SDSL-EPR to determine the conformational dynamics of His-Ste14 with and without SAM. EPR is an attractive method to study conformational changes of proteins as it is done in solution and requires relatively small amounts of protein. We generated a library of 46 non-conserved single cysteine mutants introduced into cysteine-less His-Ste14 (His-Ste14-TA). The cysteine residues engineered into His-Ste14-TA were in the cytosolic portion of the protein to ensure efficient labeling and were tested for methyltransferase activity levels. From crude membranes, only nineteen mutants retained activity levels of ≥50% of His-Ste14-TA, which were then purified and tested for methyltransferase activity levels. Eight purified mutants were selected as candidates for EPR with activity levels of ≥50% of His-Ste14-TA. Once optimized, we introduced a nitroxide spin label, 1-oxyl-2,2,5,5-tetrametylpyrroline-3-methyl)-methanethiosulfonate (MTSL), to several of the purified single cysteine mutants. Then, we evaluated protein dynamics during the methylation reaction by monitoring mobility of the MTSL-labelled residue upon addition of SAM. Overall, our structural and biochemical analyses will be used to ascertain the structural dynamics associated with SAM binding of this unique methyltransferase.
Additionally, we were able to incorporate His-Ste14 in nanodiscs. Nanodiscs mimic the membrane of a cell and are a more native-like environment that detergent micelles or liposomes. Since nanodiscs are conducive to many biophysical techniques, unlike detergents, we have begun preliminary studies to better understand the structure of Ste14. Techniques we have begun to pursue are negative stain electron microscopy (EM), single particle cryo-electron microscopy (cryo-EM), and X-ray crystallography.
Finally, we previously showed Ste14 functions as a dimer or higher order oligomer. Ste14 is comprised of six transmembrane (TM) domains in which TM1 contains a putative dimerization motif, G31XXXG35XXXG39, where G is a glycine amino acid residue and X is a subset of hydrophobic amino acids. Using cysteine-scanning mutagenesis, we characterized TM1 cysteine mutants for their effects on protein expression, activity, and stability. We determined residues S27, Y28, L30, G31, G35, and G39 are critical for maintaining activity levels. Additionally, residues M25, T26, Y28, F41, P42, and Q43 were found to form strong dimers through the addition of sulfhydryl specific cross-linkers and immunoblot analysis. Recently, the purification of dimeric Ste14 from aggregated protein components via size exclusion chromatography (SEC) was improved for further experimentation. The purified, monodispersed, His-Ste14 underwent size exclusion chromatography (SEC), multi-angle light scattering (MALS) and small-angle X-ray scattering (SAXS) to confirm the dimerization state of Ste14. Together, we have used many biochemical and biophysical methods to gain insight about the structure, function, and mechanism of Ste14. Ultimately, our studies will be utilized to design more potent therapeutics to minimize K-Ras signaling in cancer cells.
Pacheco, Bruno Filipe da Silva. "The Effect of Acupuncture at St34 on The Patellar Reflex." Master's thesis, 2015. https://repositorio-aberto.up.pt/handle/10216/82513.
Повний текст джерелаPacheco, Bruno Filipe da Silva. "The Effect of Acupuncture at St34 on The Patellar Reflex." Dissertação, 2015. https://repositorio-aberto.up.pt/handle/10216/82513.
Повний текст джерелаNunnelly, Luke Frazier. "St18 specifies MGE lineage parvalbumin expressing prototypic neurons of the globus pallidus pars externa." Thesis, 2021. https://doi.org/10.7916/d8-yeqs-hj91.
Повний текст джерелаLiu, Alice, and 劉美倩. "Potential bone healing by ST1 through osteogenesis in OVX-SAMP8 mice." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/34741954091822783811.
Повний текст джерела臺北醫學大學
生醫材料暨組織工程研究所
103
Antrodia camphorata (A C) is a medicinal fungus that has previously demonstrated many beneficial properties such as cytotoxicity to cancer cells and amelioration of inflammation syndromes. This study evaluates pure compound extracts of A C for osteogenesis, osteoporosis recovery, and osteoporotic bone injury healing effects in vitro with preosteoblast cells (MC3T3) and in vivo with an osteoporosis mouse model established in our previous studies, ovariectomied senescence accelerated mice (OVX-SAMP8). This study demonstrated that ST1 is a well-defined pure compound of A C that exhibits low cytotoxicity to preosteoblasts at 25 ug/mL, while osteogenic gene expression (RUNX2, OPN and OCN) is significantly up-regulated, and revealed an increased ratio of OPG to RANKL, indicating maintenance of the bone matrix through inhibition of osteoclastic pathway. Additionally, evaluation by Alizarin Red S staining showed increased mineralization in ST1 treated preosteoblasts. In vitro results show that ST1 could promote osteogenesis and inhibit bone digestion. In vivo testing examined both long term and short term effects of ST1 on OVX-SAMP8 mice. Short term treatment was evaluated on an osteoporotic bone injury model. Our results showed that ST1 administered by i.p. injection every other day for 2 weeks following a bone injury improved the rate of bone healing when compared to Sham group. In our long term study, OVX-SAMP8 mice were treated orally with ST1 every other day for 4 months. Osteogenesis was improved in BMSCs from the ST1 treated mice, however, possible side effects were observed. ST1 could be considered for short term osteoporosis or osteoporotic fracture therapy through anabolic action on bone health.
Hepnar, David. "Genová exprese porinů a beta-laktamáz během účinku beta-laktamových antibiotik v závislosti na velikosti inokula u klinických izolátů Klebsiella pneumoniae." Master's thesis, 2011. http://www.nusl.cz/ntk/nusl-312464.
Повний текст джерелаTai-IHsu and 許太乙. "To investigate the role of STK4 defect to promote premature aging and tumor progression in C. elegans and human cancer models." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/27144398240525778179.
Повний текст джерела國立成功大學
基礎醫學研究所
104
STK4 is a serine/threonine kinase protein encoded for 487 amino acids which plays an important role in Hippo pathway involved in development progression. Disease associated with STK4 down-regulation induces tumor recurrent and hepatocellular carcinoma formation. However, the regulation mechanism of protein expression and function are still not clear. To address the STK4 protein function in the creatures that model organism C.elegans were used to answer this question. The intestinal tract distance of cst-1 knock-out has wider than control group and premature aging has been shown. -catenin homologous bar-1 is also involved in intestinal tract development regulation mechanism. The premature aging and intestine abnormality can be restored under bar-1 down-regulated with cst-1 over-expressed. STK4 protein expression level regulation mechanism is still unknown. One possibility is post-transcriptional regulation through miRNA targeting mRNA 3’UTR degradation. The function assay and clinical samples showed that miR-18a indeed is an oncomiR to suppress STK4 induced apoptosis cascade through AKT phosphorylation regulation. Above the data from C. elegans,beta-catenin can be the candidate target interacted with STK4 in the same axis to regulate digestive tract development. IHC staining of different kinds of tumor parts showed that STK4 are highly expressed in prostate, liver and colon compared with normal specimens. All of our study indicated that STK4 is a tumor suppressor interacted with beta-catenin in prostate and colon cancers. The protein synthesis is through miRNA mediated degradation. The study demonstrated that STK4 can be the therapeutic target for prostate and colon cancer.
Kleißler, Felix. "Towards Solid-State Spin Based, High-Fidelity Quantum Computation." Doctoral thesis, 2018. http://hdl.handle.net/11858/00-1735-0000-002E-E51C-0.
Повний текст джерелаHogel, Matthew. "INVESTIGATING THE MECHANISM OF PROMOTER-SPECIFIC N-TERMINAL MUTANT HUNTINGTIN-MEDIATED TRANSCRIPTIONAL DYSREGULATION." 2011. http://hdl.handle.net/10222/15723.
Повний текст джерела