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Статті в журналах з теми "Spodoptera Litura Nuclear Polyhedrosis Virus"

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Автор: Grafiati

Опубліковано: 6 вересня 2023

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1

Syahroni, M. Novel Ghufron, and Nanang Tri Haryadi. "Uji Efektivitas Konsentrasi Spodoptera litura – Nuclear Polyhedrosis Virus (SlNPV) JTM 97C Formulasi Bubuk Terhadap Larva Spodoptera litura Fabricius (Lepidoptera: Noctuidae) Pada Tanaman Kedelai." Jurnal Pengendalian Hayati 2, no. 2 (September 25, 2019): 46. http://dx.doi.org/10.19184/jph.v2i2.17140.

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Spodoptera litura (armyworm) is an important leaf pest that is polyphagic and has a broad range of hosts. Spodoptera litura is the main pest in soybean plants and can cause up to 50% damage and often results in decreased productivity and even crop failure. Spodoptera litura is widespread in areas with hot and humid climates from the subtropics to the tropics. S. litura pests attack cultivation plants in the vegetative and generative phases. The prospect of insect pathogens as a substitute for chemical insecticides is quite good in terms of their effectiveness and impact on the environment. Nuclear Polyhedrosis Virus (NPV) is one of the Baculoviridae viruses that infect the larval stage. Spodoptera litura Nuclear Polyhedrosis Virus (SlNPV) is a pathogenic virus that infects armyworms. SlNPV JTM 97C is an effective isolate in controlling larvae of Spodoptera litura. The larvae used in this study were larval instar 4, instar 5, and instar 6. The study was conducted using a Completely Randomized Design (CRD) with 6 treatments and 3 replications. The treatments consisted of controls, 1 g / l, 2g / l, 3 g / l, 4 g / l, and 5 g / l. Each treatment was applied to instar 4, instar 5 and 6 instar larvae. The results showed that SlNPV JTM 97C affected mortality, stopped eating and pupa formation of instar 4, instar 5 larvae, but did not affect instar 6 larvae. The younger the larval instar the higher the pathogenicity response.

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2

Hasanah, Uswatun, and Nanang Tri Haryadi. "EFEKTIVITAS BAHAN PELINDUNG Spodoptera litura NUCLEAR POLYHEDROSIS VIRUS (SlNPV) UNTUK MENGENDALIKAN Spodoptera litura (F.) PADA TANAMAN KEDELAI." Jurnal Hama dan Penyakit Tumbuhan 10, no. 3 (September 16, 2022): 148–55. http://dx.doi.org/10.21776/ub.jurnalhpt.2022.010.3.5.

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Spodoptera litura dapat menyebabkan kehilangan hasil hingga 80% bahkan gagal panen. Nuclear Polyhedrosis Virus merupakan agensi hayati dari kelompok patogen serangga famili Baculoviridae yang banyak digunakan sebagai agens hayati dan Spodoptera litura Nuclear Polyhedrosis Virus (SlNPV) merupakan salah satu pemanfaatan NPV yang efektif dalam mengendalikan Spodoptera litura. Kendala penggunaan SlNPV yaitu tidak tahan terhadap sinar matahari sehingga perlu bahan pelindung agar virus tidak mengalami inaktif. Penelitian ini bertujuan untuk mengetahui pengaruh bahan pelindung dalam mempertahankan efektivitas SlNPV dari paparan sinar matahari. Penelitian dilakukan di rumah kaca dengan menggunakan Rancangan Acak Lengkap (RAL) non faktorial. Perlakuan meliputi SlNPV 100 ml tanpa pemaparan UV A (a), SlNPV 100 ml tanpa bahan pelindung + UV A (b), SlNPV 100 ml + Molase 40% + UV A (c), SlNPV 100 ml + Kaolin 40% + UV A (d), SlNPV 100 ml + Tinopal 1% + UV A (e), SlNPV 100 ml + Sunblock SPF 50 5% + UV A (f). Setiap perlakuan diulang sebanyak 4 kali sehingga didapatkan 24 satuan percobaan. Hasil penelitian menunjukkan bahwa pada uji skala rumah kaca perlakuan SlNPV + kaolin 40% dapat menyebabkan mortalitas S. litura 92,5% diikuti oleh perlakuan SlNPV + molase 40% dengan mortalitas sebesar 75% pada pengamatan 9 hari setelah aplikasi. Kaolin efektif digunakan sebagai bahan pelindung SlNPV dibandingkan bahan pelindung lainnya karena dapat melindungi SlNPV dari paparan sinar Ultra Violet (UV) karena membentuk lapisan seperti lapisan film.

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3

Monobrullah, M. D., and Masao Nagata. "EFFECTS OF LARVAL AGE ON SUSCEPTIBILITY OF SPODOPTERA LITURA (LEPIDOPTERA: NOCTUIDAE) TO SPODOPTERA LITURA MULTIPLE NUCLEAR POLYHEDROSIS VIRUS." Canadian Entomologist 132, no. 3 (June 2000): 337–40. http://dx.doi.org/10.4039/ent132337-3.

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Susceptibility of many insects to baculoviruses decreases with host development (Hochberg 1991), whereas the time required for infected hosts to die increases with larval age (Boucias et al. 1980; Bucher and Turnock 1983; Smits and Vlak 1988). The actual mechanism of this phenomenon, however, has not been established in many cases. The present study was undertaken to investigate the effect of larval age on the susceptibility of Spodoptera litura Fabricius to nuclear polyhedrosis virus (NPV) through oral and intrahaemocoelic routes of infection.

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4

Afidah, Nafisatul, Tutung Hadiastono, Bedjo Bedjo, and Fery Abdul Choliq. "Compatibility of Lemongrass Extract with Spodoptera litura Nuclear Polyhedrosis Virus Against Spodoptera litura on Soybean Plants." Journal of Tropical Plant Protection 2, no. 1 (January 1, 2021): 7–13. http://dx.doi.org/10.21776/ub.jtpp.2021.002.1.2.

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Armyworm (Spodoptera litura) is an important pest that damages the leaves of soybean. SlNPV is one type of pathogen that has potential as a biocontrol agent to control armyworms because it is specific, selective, and effective. This study aimed to investigate the compatibility between the lemongrass and SlNPV in controlling armyworm. The Research was conducted at the Laboratory of Indonesian Legumes and Tuber Crops Research Institute (ILETRI) from February 2016 until April 2016. The study using completely randomized design with 7 treatments and 3 replication with a treatment that consists of control, SlNPV-JTM 97C 1,5 × 106 PIB/ml, 10% of lemongrass extract, a combination SlNPV-JTM 97C fixed concentrate of 1,5 × 106 PIB/ml with 2.5%, 5%, 7.5%, and 10% of lemongrass extract. The results showed that the combination of SlNPV-JTM 97C 1.5 × 106 PIB/ml with 10% lemongrass had the highest level of virulence, with 96% of mortality on larva 100% on the pupa stage, so that the combination of SlNPV and lemongrass extracts was compatible in controlling S.litura.

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5

Maeda, S., Y. Mukohara, and A. Kondo. "Characteristically distinct isolates of the nuclear polyhedrosis virus from Spodoptera litura." Journal of General Virology 71, no. 11 (November 1, 1990): 2631–39. http://dx.doi.org/10.1099/0022-1317-71-11-2631.

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6

Das, Rakha, and Y. Durga Prasad. "Restriction endonuclease analysis of a Spodoptera litura nuclear polyhedrosis virus (NPV) isolate." IUBMB Life 39, no. 1 (May 1996): 1–11. http://dx.doi.org/10.1080/15216549600201001.

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7

Im, Dae Joon, B. M. Shepard, and R. M. Aguda. "Pathogenicity and Histopathology of a Nuclear Polyhedrosis Virus of Spodoptera litura (Fab.)." International Journal of Tropical Insect Science 9, no. 04 (August 1988): 539–42. http://dx.doi.org/10.1017/s1742758400011115.

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8

N, DHANDAPANI, and SUNDARA BABU P.C. "EFFICACY OF NUCLEAR POLYHEDROSIS VIRUS AGAINST Spodoptera litura ON GROUNDNUT IN DIFFERENT LOCATIONS OF TAMIL NADU." Madras Agricultural Journal 82, september October (1995): 531–33. http://dx.doi.org/10.29321/maj.10.a01256.

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Field trials conducted against Spodoptera litura (Fb.) with nuclear polyhedrosis virus (NPV) on groundnut (Arachis hypogaea L.) in eight different locations of Tamil Nadu revealed that two rounds of application of NPV at 250 Larval equivalent (LE)/ha with the adjuvant crude sugar at 2.5 kg/ha at ten days interval was significantly superior than untreated check in reducing the larval population and increasing the pod yield. irrespective of the locations tested.

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9

Miranti, Mia, Wawan Hermawan, Melanie ., Rahman Perdana Hadi, Dicky Budi Sugiarto, and Desy Efriyani Anggraeny Nasution. "THE POTENTIAL OF SUBCULTURE Helicoverpa armigera NUCLEAR POLYHEDROSIS VIRUS (HaNPV) TO BE UTILIZED AS AN ALTERNATE SYNTHETIC INSECTICIDES TO CONTROL INSECTS PESTS IN CABBAGES PLANTATION (Brassica oleracea var. capitata L)." KnE Life Sciences 2, no. 1 (September 20, 2015): 405. http://dx.doi.org/10.18502/kls.v2i1.183.

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<p>Subculture Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV) is an entomopathogenic viruses isolated from cadaver of Helicoverpa armigera larvae, and was succesfully produced in Spodoptera litura larvae as an alternate host. These viral agent have been applied to control the population of three species insects pests Spodoptera litura, Crocidolomia pavonana and Plutella xyllostella which were infested in Cabbages Plantation. The concentration of virus of 4 x 107 Polyhedral/ml was sprayed in cabbage leaves 24 hours before infested of larval and every four days after. The research used randomized design which consisted of factor (three species of second instar larvae, S. litura, C. pavonana, P. xyllostella) and eight replications. The result showed that the three species of larval were sensitive against subculture HaNPV infection. The mortality of each larval species (S. litura, C. pavonana and P. xyllostella) were 100%, 97.5% and 98.7%. Subculture HaNPV can be utilized to control the population of three spesies of insects pests in Cabbage Plantation. </p><p>Keywords : Subculture HaNPV, Polyhedral</p>

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10

Gatehouse, L. N., N. P. Markwick, J. Poulton, and J. T. Christeller. "Avidin expression does not increase speed of kill of Spodoptera litura by baculovirus." New Zealand Plant Protection 56 (August 1, 2003): 194–200. http://dx.doi.org/10.30843/nzpp.2003.56.6042.

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A disarmed (polhminus) Autographa californica nuclear polyhedrosis virusbased baculovirus expressing green fluorescent protein (GFP) on the strong late promotor p10 was constructed A second virus was made which also expressed the biotinbinding protein avidin from the strong late promotor polh Both viruses caused strong fluorescence in Spodoptera frugiperda cells in insect cell culture Sevendayold larvae of Spodoptera litura were inoculated by microinjection with GFP virus GFPavidin virus or tissue culture medium (control) Mortality growth rate and the development of green fluorescence were followed and after death the larvae were analysed for GFP and avidin content All virusinjected larvae developed strong fluorescence However no significant differences in growth or mortality were seen between the larvae injected with virus expressing and not expressing avidin Both GFP and avidin accumulated to high levels in the larvae The expression of avidin did not change the level of expression of GFP

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11

Erdiansyah, I., M. Syarief, and R. Taufika. "Virulence of Spodoptera Litura Nuclear Polyhedrosis Virus (SLNPV) with kaolin as carrier material on spodoptera litura and tetragonula laeviceps on soybean." IOP Conference Series: Earth and Environmental Science 672, no. 1 (March 1, 2021): 012097. http://dx.doi.org/10.1088/1755-1315/672/1/012097.

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12

Pramono, Sudi. "EFEK PENYIMPANAN TERHADAP VIRULENSI NUCLEAR POLYHEDROSIS VIRUS YANG DIAPLIKASIKAN PADA TANAMAN KEDELAI TERSERANG ULAT GRAYAK (Spodoptera litura Fabr. )." Jurnal Hama dan Penyakit Tumbuhan Tropika 1, no. 1 (March 2, 2001): 29–32. http://dx.doi.org/10.23960/j.hptt.1129-32.

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Анотація:

One of the problem increased soybean production was Spodoptera litura (army worm), because army worm has been known to be resistant to many insec¬ticides. Alternatif control techniques should be explored to reduce population of the pest. Nuclear Polyhedrosis Virus (NPV) could storage one or more year, but must used technique cold storage so that not virulence decreased. This experiment was conducted to study (1) NPV patho¬genicity to army worm in the field, and (2) the effect of storage on the pathogenicity of NPV to S. litura. The research consisted of two experiments i.e. laboratory and field experiments. Each experiment had seven treatments arranged in a randomized completely block design. Each treatment was replicated four times. The results show the highest mortality of S. litura occurred in plot treated with 1,0 x 107 PIBs /ml, the mortality was 48 to 49,33 spec/plot after ten days aplicated . If comparative patogenicity the new and the old NPV was not significant. Population of army worm decreased significantly by NPV and control. So that patogenecity NPV storage one year as well as new NPV against army worm.

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13

Uge, Emerensiana, Eriyanto Yusnawan, and Yuliantoro Baliadi. "Pengendalian Ramah Lingkungan Hama Ulat Grayak (Spodoptera litura Fabricius) pada Tanaman Kedelai." Buletin Palawija 19, no. 1 (May 31, 2021): 64. http://dx.doi.org/10.21082/bulpa.v19n1.2021.p64-80.

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Spodoptera litura Fabricius (Lepidoptera: Noctuidae) atau dikenal dengan ulat grayak merupakan hama penting pada tanaman kedelai dan beberapa jenis tanaman penting di Indonesia. Serangan S. litura dapat mengakibatkan kerusakan, bahkan kehilangan hasil pada tanaman kedelai. Gejala serangan berupa daun berlubang karena larva memakan jaringan daun hingga menyisakan epidermis dan tulang daun. Hama ini dilaporkan menyerang tanaman kedelai di sentra-sentra produksi di Indonesia yaitu Aceh, Jawa Barat, Jawa Tengah, Jawa Timur, Nusa Tenggara Barat, Sulawesi Selatan dan dan Sulawesi Tengah. Upaya pengendalian hama ulat grayak yang dilakukan petani adalah menggunakan pestisida sintetik, namun karena dampak negatif penggunaannya terhadap kesehatan manusia dan keseimbangan ekosistem alam, maka perlu adanya alternatif pengendalian yang dampak negatifnya rendah terhadap lingkungan. Beberapa teknologi pengendalian yang telah diteliti dan diketahui efektifitasnya antara lain; penggunaan Spodoptera litura nuclear polyhedrosis virus (SlNPV) (50-100%), cendawan entomopatogen Beauvaria bassiana (51-93%), Metarhizium anisopliae (93-100%), Nomuraea rileyi dan Lecanicillium lecanii (80-85%), Parasitoid (13-56%), predator Forficula auricularia (96%), nematoda entomopatogen Steinernematidae (30-51%), pestisida nabati (>30%), tanaman perangkap dan varietas tahan Aplikasi yang tepat akan mendukung perkembangbiakan spesies tersebut di alam sehingga akan terjadi siklus rantai makanan yang seimbang dan berkelanjutan. Komponen pengendalian ini dapat dimasukkan dalam pengendalian terpadu, sehingga serangan S. litura dapat ditekan, tanaman berproduksi optimal, keseimbangan ekosistem dapat dipertahankan, residu pestisida dapat diturunkan dan sistem pertanian berkelanjutan tercapai.

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14

Liao, Z. H., T. C. Kuo, C. W. Shih, S. J. Tuan, Y. H. Kao, and R. N. Huang. "Effect of juvenile hormone and pyriproxyfen treatments on the production of Spodoptera litura nuclear polyhedrosis virus." Entomologia Experimentalis et Applicata 161, no. 2 (October 28, 2016): 112–20. http://dx.doi.org/10.1111/eea.12499.

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15

Nasution, Desy Efriyani Anggraeny, Mia Miranti, and Melanie . "Biological Test of Formulation of Subculture Helicovepa armigera Nuclear Polihedrosis Virus (HaNPV) on Mortality of Spodoptera litura Larvae infested to cabbage (Brassica oleracea Var. capitata Linn.) Plantation." KnE Life Sciences 2, no. 1 (September 20, 2015): 646. http://dx.doi.org/10.18502/kls.v2i1.237.

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<p>Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV) is one of the biological agents that could be used for the development of organic farming in Indonesia. One of the organic agricultural food products which have high economic value is cabbage, HaNPV effectif to control the population of several species of insect pests, can be used instead of synthetic insecticides in agricultural development efforts of organic cabbage. HaNPV formulation can be used as a way to maintain the virulence properties of the virus and facilitate the storage and application. The preparation of subculture HaNPV formulations was in liquid, powders, cornstarch and talk carrier material. The design experimental was used randomized block design consists single factor (type HaNPV formulation consisting of powder formulations, liquid formulation, formulation in cornstarch, and talc formulation including water formulation without virus as a control), with eight replications. The results showed that all of the formulations of subculture HaNPV causing larvae mortality at range 97.4% -100%. The highest mortality of larvae occurred in cabbage plants are protected by HaNPV carrier liquid and talk preparations by 100% each. <br /><strong></strong></p><p><strong>Keywords</strong>: Formulations Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV), subculture, Spodoptera litura, Brassica oleracea Var. capitata Linn.</p>

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16

Kurnianto, Agung Sih, Ana Miftahul Janah, Nur Laila Magvira, and Ankardiansyah Pandu Pradana. "Effects of Nuclear Polyhedrosis Virus (NPV) on The Death of Spodoptera litura Larvae (Lepidoptera: Noctuidae) and its Comparison with Chemical Pesticide." Techno Jurnal Penelitian 11, no. 1 (May 30, 2022): 47–54. http://dx.doi.org/10.33387/tjp.v11i1.3315.

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Spodoptera litura larvae are polyphagous insects and have caused a lot of damage to various commodities. The application of Nuclear Polyhedrosis Virus (NPV) can be used as an alternative to control, other than by using chemical pesticides. This research was conducted to compare the mortality of S. litura with NPV treatment and compare them with chemical pesticides. NPV treatment with a concentration of 0.3% is used to soak the leaves to be used as larvae feed, as well as chemical pesticides the active ingredient Lambda-cyhalothrin (with a concentration of 0.05%). Observations were made at 2, 24, 48, and 76 hours after application. The parameters observed from the treatment included the percentage of mortality and morphological changes of larvae. Observation of the morphological changes of larvae was carried out by the parameters of slowing motion, discoloration, decaying, and severe shrinkage. Based on the results of the comparison, the percentage of mortality in the treatment of chemical pesticides is higher than the NPV. Death of 100% in Lambda-Cyhalothrin treatment was recorded in the first 2 (two) hours, while for NPV treatment is as much as 80%. The larvae with NPV treatment undergo a process of color change and the decomposition process is faster than the treatment of Lambda-cyhalothrin.

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17

Behera, Aruna K., Mukesh Kumar, Anju Bansal, Om B. Bansal, and Rakha H. Das. "Expression of lacZ reporter gene under the control of the polyhedrin promoter of Spodoptera litura nuclear polyhedrosis virus." Gene 190, no. 1 (January 1997): 145–50. http://dx.doi.org/10.1016/s0378-1119(96)00745-7.

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18

Biswakarma, Dipen, Nilanjan Dey, Deepa Bhagat, and Santanu Bhattacharya. "Switchable Luminescent Probe for Trace-Level Detection of the Spodoptera litura Nuclear Polyhedrosis Virus via a Color-Changing Response." ACS Agricultural Science & Technology 1, no. 4 (May 14, 2021): 322–28. http://dx.doi.org/10.1021/acsagscitech.1c00009.

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19

SHIH, Cheng-Jen, and Jui-Chu CHANG. "A Simple Media for Growth of Spodoptera litura (Lepidoptera: Noctuidae) Cells and Propagation of Autographa californica Nuclear Polyhedrosis Virus." Applied Entomology and Zoology 32, no. 4 (1997): 589–94. http://dx.doi.org/10.1303/aez.32.589.

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20

Liu, Kaiyu, Duanyang Shu, Na Song, Zhongchao Gai, Yuan Yuan, Juan Li, Min Li, Shuying Guo, Jianxin Peng, and Huazhu Hong. "The Role of Cytochrome c on Apoptosis Induced by Anagrapha falcifera Multiple Nuclear Polyhedrosis Virus in Insect Spodoptera litura Cells." PLoS ONE 7, no. 8 (August 28, 2012): e40877. http://dx.doi.org/10.1371/journal.pone.0040877.

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21

Liu, Lijun, Jianxin Peng, Kaiyu Liu, Hong Yang, Yi Li, and Huazhu Hong. "Influence of cytochrome c on apoptosis induced by Anagrapha (Syngrapha) falcifera multiple nuclear polyhedrosis virus (AfMNPV) in insect Spodoptera litura cells." Cell Biology International 31, no. 9 (September 2007): 996–1001. http://dx.doi.org/10.1016/j.cellbi.2007.03.011.

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22

Prayogo, Yusmani, and Marida Santi Yudha Ika Bayu. "Pengembangan teknologi pengendalian hama utama kacang hijau menggunakan biopestisida." Jurnal Entomologi Indonesia 17, no. 2 (September 14, 2020): 70. http://dx.doi.org/10.5994/jei.17.2.70.

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<p>Pests are one of the main obstacles in improving mungbean production in Indonesia. Currently, conventional control by using synthetic pesticides is less successful because the population and damage due to the organism are still high. This study aims to evaluate the mungbean pest control technology by using biopesticide. The experiment was conducted using randomized block design, five treatments and five replicates. The treatments were P1: preventive of neem seed powder (NSP), entompothogenic virus which contain <em>Spodoptera litura</em> nuclear polyhedrosis virus (Virgra), and conidia of entomopathogenic fungi <em>Beauveria bassiana</em> (BeBas) application; P2: SBM, Virgra, and BeBas application based on economic threshold (ET); P3: application of synthetic pesticides based on schedule; P4: application of synthetic pesticides based on ET; and P5: without control. The results showed that the pests were armyworm (<em>Spodoptera litura </em>(Fabricius)), whitefly (<em>Bemisia tabaci </em>(Gennadius)), <em>Empoasca</em> sp., <em>Megalurothrips</em> <em>usitatus</em> (Bagnall), brown stink bug (<em>Riptortus</em> <em>linearis </em>(Fabricius)), green stink bug (<em>Nezara viridula </em>(Linnaeus)), and pod borer (<em>Maruca testulalis </em>(Geyer)). The application of NSP, Virgra, and BeBas, as preventive measures and the application of synthetic pesticides based on schedule effectively were suppressed pest population. In contrast, the application of biopesticides and synthetic pesticides based on ET did not suppress the development of major pests. The application of biopesticide based on ET are safe for the survival of natural enemies. Meanwhile, the application of synthetic pesticides can destroy existing natural enemies. Integrated management of NSP, Virgra, and BeBas, that are applied preventively are potential to be innovative technology for controlling the major mungbean pest to replace synthetic pesticides.</p>

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23

MASUDA, Toshio, Hidenori IWAHANA, and Kisaku AKUTU. "Studies on transovum transmission of the nuclear polyhedrosis virus of Spodoptera litura (SiNPV). I. Infection of SlNPV to the next generation larvae through the male moths contaminated with SlNPV." Japanese journal of applied entomology and zoology 34, no. 1 (1990): 1–6. http://dx.doi.org/10.1303/jjaez.34.1.

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24

BEDJO, BEDJO. "Various isolates of Spodoptera litura Nuclear Polyhedrosis Viruses from East Java (Indonesia) to control Spodoptera litura on soybean." Biodiversitas, Journal of Biological Diversity 18, no. 2 (May 1, 2017): 582–88. http://dx.doi.org/10.13057/biodiv/d180219.

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25

Al Fazairy, Ahlam A., and F. A. Hassan. "Infection of Termites by Spodoptera littoralis Nuclear Polyhedrosis Virus." International Journal of Tropical Insect Science 9, no. 01 (February 1988): 37–39. http://dx.doi.org/10.1017/s1742758400009991.

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26

Grzywacz, D., K. A. Jones, G. Moawad, and A. Cherry. "The in vivo production of Spodoptera littoralis nuclear polyhedrosis virus." Journal of Virological Methods 71, no. 1 (March 1998): 115–22. http://dx.doi.org/10.1016/s0166-0934(97)00209-7.

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27

Fuxa, J. R., and A. R. Richter. "Reversion of resistance by Spodoptera frugiperda to nuclear polyhedrosis virus." Journal of Invertebrate Pathology 53, no. 1 (January 1989): 52–56. http://dx.doi.org/10.1016/0022-2011(89)90073-6.

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28

Danyluk, Gregory M., and James E. Maruniak. "In vivo and in vitro host range of Autographa californica nuclear polyhedrosis virus and Spodoptera frugiperda nuclear polyhedrosis virus." Journal of Invertebrate Pathology 50, no. 3 (November 1987): 207–12. http://dx.doi.org/10.1016/0022-2011(87)90084-x.

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29

Liu, J. Jian, and Eric B. Carstens. "Infection of Spodoptera frugiperda and Choristoneura fumiferana cell lines with the baculovirus Choristoneura fumiferana nuclear polyhedrosis virus." Canadian Journal of Microbiology 39, no. 10 (October 1, 1993): 932–40. http://dx.doi.org/10.1139/m93-141.

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Анотація:

The growth properties of cell lines derived from Spodoptera frugiperda (alfalfa looper) and Choristoneura fumiferana (spruce budworm) were investigated. The data demonstrated that the spruce bud worm cell line grew more slowly than the alfalfa looper cell line, and this reduced growth rate appeared to affect the rate of baculovirus replication in infected cells. Trypsinizing the spruce budworm cells or varying the multiplicity of infection did not greatly influence the rate of viral replication. Autographa californica nuclear polyhedrosis virus was able to replicate its DNA and synthesize late and very late proteins in both cell lines but did not produce infectious extracellular virus in the spruce budworm cell line. The replication cycle of C. fumiferana nuclear polyhedrosis virus did not produce late proteins or infectious extracellular virus in the alfalfa looper cells. The results indicate that S. frugiperda cells are nonpermissive for the C. fumiferana nuclear polyhedrosis virus but C. fumiferana cells are semipermissive for the A. californica nuclear polyhedrosis virus, resulting in an abortive infection.Key words: baculovirus, host specificity, AcMNPV, CfMNPV, spruce budworm.

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30

Hara, Koyu, Masako Funakoshi, Katsuo Tsuda, and Takeshi Kawarabata. "New spodoptera exigua cell lines susceptible toSpodoptera exigua nuclear polyhedrosis virus." In Vitro Cellular & Developmental Biology - Animal 29, no. 12 (December 1993): 904–7. http://dx.doi.org/10.1007/bf02634225.

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31

Gothama, A. A. Agra, P. P. Sikorowski, and G. W. Lawrence. "Interactive Effects of Steinernema carpocapsae and Spodoptera exigua Nuclear Polyhedrosis Virus on Spodoptera exigua Larvae." Journal of Invertebrate Pathology 66, no. 3 (November 1995): 270–76. http://dx.doi.org/10.1006/jipa.1995.1100.

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32

Smits, P. H., M. Vrie, and J. M. Vlak. "Nuclear polyhedrosis virus for control of Spodoptera exigua larvae on glasshouse crops." Entomologia Experimentalis et Applicata 43, no. 2 (April 1987): 73–80. http://dx.doi.org/10.1111/j.1570-7458.1987.tb01049.x.

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33

Smits, P. H., M. Vrie, and J. M. Vlak. "Nuclear polyhedrosis virus for control of Spodoptera exigua larvae on glasshouse crops." Entomologia Experimentalis et Applicata 43, no. 1 (February 1987): 73–80. http://dx.doi.org/10.1111/j.1570-7458.1987.tb02205.x.

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34

Croizier, G., K. Boukhoudmi-Amiri, and L. Croizier. "A physical map of Spodoptera littoralis B-type nuclear polyhedrosis virus genome." Archives of Virology 104, no. 1-2 (March 1989): 145–51. http://dx.doi.org/10.1007/bf01313815.

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35

Smits, P. H., and J. M. Vlak. "Biological activity of Spodoptera exigua nuclear polyhedrosis virus against S. exigua larvae." Journal of Invertebrate Pathology 51, no. 2 (March 1988): 107–14. http://dx.doi.org/10.1016/0022-2011(88)90066-3.

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36

Shapiro, D. I., J. R. Fuxa, H. D. Braymer, and D. P. Pashley. "DNA restriction polymorphism in wild isolates of Spodoptera frugiperda nuclear polyhedrosis virus." Journal of Invertebrate Pathology 58, no. 1 (July 1991): 96–105. http://dx.doi.org/10.1016/0022-2011(91)90167-o.

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37

Tsuda, Katsuo, Eiichi Mizuki, Takeshi Kawarabata, and Keio Aizawa. "Comparative neutralization of nuclear polyhedrosis viruses infectious to the tobacco cutworm, Spodoptera litura (Lepidoptera: Noctuidae), in a cell line of Spodoptera frugiperda." Journal of Invertebrate Pathology 52, no. 2 (September 1988): 248–52. http://dx.doi.org/10.1016/0022-2011(88)90132-2.

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38

KONDO, Atushi, Takeshi NAKADA, and Syuiti HIRAI. "Control of Plutella xylostella by Spodoptera exigua nuclear polyhedrosis virus in field traial." Annual Report of The Kansai Plant Protection Society 39 (1997): 13–14. http://dx.doi.org/10.4165/kapps1958.39.0_13.

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39

Elnagar, Salah, M. F. S. Tawfik, and T. Abdelrahman. "The susceptibility to nuclear polyhedrosis virus among laboratory population of Spodoptera littoralis (Boisd.)." Zeitschrift für Angewandte Entomologie 96, no. 1-5 (August 26, 2009): 459–63. http://dx.doi.org/10.1111/j.1439-0418.1983.tb03699.x.

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40

Roberts, P. L. "Infectivity assays for the Autographa californica nuclear polyhedrosis virus using Spodoptera littoralis cells." Journal of Virological Methods 10, no. 1 (January 1985): 1–10. http://dx.doi.org/10.1016/0166-0934(85)90082-5.

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41

Caballero, P., D. Zuidema, C. Santiago‐Alvarez, and J. M. Vlak. "Biochemical and biological characterization of four isolates of Spodoptera exigua nuclear polyhedrosis virus." Biocontrol Science and Technology 2, no. 2 (January 1992): 145–57. http://dx.doi.org/10.1080/09583159209355228.

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42

Fuxa, J. R. "Spodoptera frugiperda Susceptibility to Nuclear Polyhedrosis Virus Isolates with Reference to Insect Migration." Environmental Entomology 16, no. 1 (February 1, 1987): 218–23. http://dx.doi.org/10.1093/ee/16.1.218.

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43

Hara, Koyu, Masako Funakoshi, and Takeshi Kawarabata. "A cloned cell line ofSpodopteta exiguahas a highly increased susceptibility to theSpodoptera exiguanuclear polyhedrosis virus." Canadian Journal of Microbiology 41, no. 12 (December 1, 1995): 1111–16. http://dx.doi.org/10.1139/m95-154.

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A cloned cell line designated Se301, derived from a continuous Se3FH cell line of the beet army worm Spodoptera exigua, showed 10 times greater sensitivity when tested with the plaque assay to a S. exigua nuclear polyhedrosis virus (SeNPV) as compared with the parent cell line. Nearly 100% of Se301 cells were infected with the plaque purified isolate SeNPV-I1 or the wild isolate SeNPV-IW. Plaques were first detected 3–4 days in the Se301 cell line, which was 1–2 days faster than the Se3FH cell line. The size of plaques formed in the Se301 cell line was markedly larger than that of the parental cell line. At 12 h post infection, the production of the extracellular viruses (ECVs) in the Se301 cell line infected with either SeNPV-I1 or SeNPV-IW was 20–40 times greater than that in the Se3FH cell line. In contrast, Se301 cells infected with SeNPV-I1 or SeNPV-IW produced polyhedral inclusion bodies (PIBs) at lower levels compared with Se3FH cells. The isolate SeNPV-I1 consistently produced more ECVs and PIBs in both the Se301 and Se3FH cell lines than the isolate SeNPV-IW.Key words: Spodoptera exigua, nuclear polyhedrosis virus, cloned cell line, increased virus sensitivity, virus production.

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44

Hamm, J. J., and J. E. Carpenter. "Compatibility of Nuclear Polyhedrosis Viruses and Inherited Sterility for Control of Corn Earworm and Fall Armyworm (Lepidoptera: Noctuidae)." Journal of Entomological Science 32, no. 2 (April 1, 1997): 148–53. http://dx.doi.org/10.18474/0749-8004-32.2.148.

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Inherited sterility has been proposed as a means of suppressing the populations of the corn earworm, Helicoverpa zea (Boddie), and the fall armyworm, Spodoptera frugiperda (J. E. Smith). If nuclear polyhedrosis viruses could be used to kill larvae, thereby reducing the number of moths in the field populations, fewer moths treated with substerilizing doses of irradiation would need to be released. However, for these two methods to be compatible, the progeny of substerile moths should be no more susceptible to the virus than the progeny of the field populations. The corn earworm nuclear polyhedrosis virus (Elcar™) was bioassayed against corn earworm larvae from untreated moths and larvae from male, female, and male and female moths treated with 100 Gy of irradiation and larvae from male moths treated with 150 Gy of irradiation. The fall armyworm nuclear polyhedrosis virus was bioassayed against fall armyworm larvae from untreated moths and larvae from male moths treated with 100 to 150 Gy of irradiation. There was no significant difference between susceptibility of larvae from untreated moths and larvae from irradiated moths. Thus, the use of nuclear polyhedrosis viruses for control of larvae should be compatible with the release of substerilized moths as part of an integrated pest management approach for area-wide management of the corn earworm and fall armyworm.

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45

HARA, Koyu, Masako FUNAKOSHI, Katsuo TSUDA, and Takeshi KAWARABATA. "Susceptibility of Lepidopteran Cell Lines to a Spodoptera exigua (Lepidoptera: Noctuidae) Nuclear Polyhedrosis Virus." Applied Entomology and Zoology 29, no. 3 (1994): 395–402. http://dx.doi.org/10.1303/aez.29.395.

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46

Moawed, Sayed M., and Ibrahim M. Elnabrawy. "Effectiveness of nuclear polyhedrosis virus and insecticides against the cotton leafworm, Spodoptera littoralis (Boisd.)." International Journal of Tropical Insect Science 8, no. 01 (February 1987): 89–93. http://dx.doi.org/10.1017/s1742758400007037.

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47

Kislev, Naomi. "DNA Homology Relationships between Spodoptera littoralis Nuclear Polyhedrosis Virus and Other Baculoviruses." Intervirology 24, no. 1 (1985): 50–57. http://dx.doi.org/10.1159/000149618.

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48

Croizier, Liliane, and Guy Croizier. "Nucleotide sequence of the polyhedrin gene of Spodoptera littoralis multiple nucleocapsid nuclear polyhedrosis virus." Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression 1218, no. 3 (August 1994): 457–59. http://dx.doi.org/10.1016/0167-4781(94)90205-4.

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49

Santiago-Alvarez, Cándido, and Enrique Vargas Osuna. "Reduction of reproductive capacity of Spodoptera littoralis males by a nuclear polyhedrosis virus (NPV)." Journal of Invertebrate Pathology 52, no. 1 (July 1988): 142–46. http://dx.doi.org/10.1016/0022-2011(88)90113-9.

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50

Chejanovsky, Nor, and Eduard Gershburg. "The Wild-Type Autographa californica Nuclear Polyhedrosis Virus Induces Apoptosis of Spodoptera littoralis Cells." Virology 209, no. 2 (June 1995): 519–25. http://dx.doi.org/10.1006/viro.1995.1284.

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