Статті в журналах з теми "Soybean – Breeding; Soybean – Drought tolerance"
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1
Pimentel, João Roberto, Ivan Ricardo Carvalho, Cristian Troyjack, Gilberto Troyjack Junior, Vinicius Jardel Szareski, Giordano Gelain Conte, Murilo Vieira Loro, Deivid Araújo Magano, and Danieli Jacoboski Hutra. "Water deficit in the soybean breeding." Agronomy Science and Biotechnology 7 (May 27, 2021): 1–20. http://dx.doi.org/10.33158/asb.r128.v7.2021.
Повний текст джерелаАнотація:
The climate unpredictability causes long periods of drought, becoming the main risk factor in soybeans production fields and consequent losses to farmers in Brazil and worldwide. As sessile organisms, plants are constantly challenged by a wide range of environmental stresses, including drought. Growth constraints and stress due to these environmental changes result in reduced yield and significant harvesting losses. The response to abiotic stresses is a very complex phenomenon, since several stages of plant development can be affected by a particular stress and often several stresses affect the plant simultaneously. In order to mitigate the damages caused by the climate, new soybean cultivars adapted to the drought and the diversified climate are necessary, as well as technological advances in the production of soybeans that must advance with the increase of cultivated area. Therefore, the mechanisms underlying tolerance and adaptation to stress have been the focus of intensive research. In this sense, the objective of this review is to provide an overview of the evolution of genetic improvement regarding the search for more drought-tolerant cultivars, as well as to verify which strategies are used in the genetic improvement of soybean in the search of these genotypes.
2
Shahriari, Amir Ghaffar, Zahra Soltani, Aminallah Tahmasebi, and Péter Poczai. "Integrative System Biology Analysis of Transcriptomic Responses to Drought Stress in Soybean (Glycine max L.)." Genes 13, no. 10 (September 26, 2022): 1732. http://dx.doi.org/10.3390/genes13101732.
Повний текст джерелаАнотація:
Drought is a major abiotic stressor that causes yield losses and limits the growing area for most crops. Soybeans are an important legume crop that is sensitive to water-deficit conditions and suffers heavy yield losses from drought stress. To improve drought-tolerant soybean cultivars through breeding, it is necessary to understand the mechanisms of drought tolerance in soybeans. In this study, we applied several transcriptome datasets obtained from soybean plants under drought stress in comparison to those grown under normal conditions to identify novel drought-responsive genes and their underlying molecular mechanisms. We found 2168 significant up/downregulated differentially expressed genes (DEGs) and 8 core modules using gene co-expression analysis to predict their biological roles in drought tolerance. Gene Ontology and KEGG analyses revealed key biological processes and metabolic pathways involved in drought tolerance, such as photosynthesis, glyceraldehyde-3-phosphate dehydrogenase and cytokinin dehydrogenase activity, and regulation of systemic acquired resistance. Genome-wide analysis of plants’ cis-acting regulatory elements (CREs) and transcription factors (TFs) was performed for all of the identified DEG promoters in soybeans. Furthermore, the PPI network analysis revealed significant hub genes and the main transcription factors regulating the expression of drought-responsive genes in each module. Among the four modules associated with responses to drought stress, the results indicated that GLYMA_04G209700, GLYMA_02G204700, GLYMA_06G030500, GLYMA_01G215400, and GLYMA_09G225400 have high degrees of interconnection and, thus, could be considered as potential candidates for improving drought tolerance in soybeans. Taken together, these findings could lead to a better understanding of the mechanisms underlying drought responses in soybeans, which may useful for engineering drought tolerance in plants.
3
Zhao, Xingzhen, Zhangxiong Liu, Huihui Li, Yanjun Zhang, Lili Yu, Xusheng Qi, Huawei Gao, Yinghui Li, and Lijuan Qiu. "Identification of Drought-Tolerance Genes in the Germination Stage of Soybean." Biology 11, no. 12 (December 13, 2022): 1812. http://dx.doi.org/10.3390/biology11121812.
Повний текст джерелаАнотація:
Drought stress influences the vigor of plant seeds and inhibits seed germination, making it one of the primary environmental factors adversely affecting food security. The seed germination stage is critical to ensuring the growth and productivity of soybeans in soils prone to drought conditions. We here examined the genetic diversity and drought-tolerance phenotypes of 410 accessions of a germplasm diversity panel for soybean and conducted quantitative genetics analyses to identify loci associated with drought tolerance of seed germination. We uncovered significant differences among the diverse genotypes for four growth indices and five drought-tolerance indices, which revealed abundant variation among genotypes, upon drought stress, and for genotype × treatment effects. We also used 158,327 SNP markers and performed GWAS for the drought-related traits. Our data met the conditions (PCA + K) for using a mixed linear model in TASSEL, and we thus identified 26 SNPs associated with drought tolerance indices for germination stage distributed across 10 chromosomes. Nine SNP sites, including, for example, Gm20_34956219 and Gm20_36902659, were associated with two or more phenotypic indices, and there were nine SNP markers located in or adjacent to (within 500 kb) previously reported drought tolerance QTLs. These SNPs led to our identification of 41 candidate genes related to drought tolerance in the germination stage. The results of our study contribute to a deeper understanding of the genetic mechanisms underlying drought tolerance in soybeans at the germination stage, thereby providing a molecular basis for identifying useful soybean germplasm for breeding new drought-tolerant varieties.
4
Sichkar, V. I., and S. M. Pasichnyk. "Genetic-physiological basis of legume crops resistance to drought stress." Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv 16, no. 1 (September 7, 2018): 35–51. http://dx.doi.org/10.7124/visnyk.utgis.16.1.901.
Повний текст джерелаАнотація:
Goal. Identify the effective criteria for drought tolerances of leguminous crops, based on their basis evaluate the recommendation for cultivating soybean cultivars and discuss the mechanisms of resistance on the organism and molecular levels. Methods. The collection and breeding genotypes of soybean, chickpea and pea were grown in field and laboratory experiments as well as cultivars included in the State register of plant varieties suitable for dissemination in Ukraine. The reaction of soybean plants on the effect of water stress was determined in climatic chambers at the temperature of 30–32 °C. Results. The genetic variability in absorption of water by seeds of various soybean cultivars was detected especially at the initial stages of soaking. The tolerance to drought is associated with the level of free proline accumulation and water-keeping proteins in the leaves, the area of the leaf surface, the loss of moisture by the plant for a certain period, the development of the root system. Soybean cultivars Arcadia odesskaya and Hodson distinguished by increased resistance to drought. Conclusions. Tested in the field and laboratory conditions methods for determining resistance to water stress may be recommended for use in breeding research with agricultural crops. Keywords: breeding of leguminous crops, drought tolerance, adaptability to high temperature.
5
Fang, Xin, Jia Ma, Fengcai Guo, Dongyue Qi, Ming Zhao, Chuanzhong Zhang, Le Wang, et al. "The AP2/ERF GmERF113 Positively Regulates the Drought Response by Activating GmPR10-1 in Soybean." International Journal of Molecular Sciences 23, no. 15 (July 24, 2022): 8159. http://dx.doi.org/10.3390/ijms23158159.
Повний текст джерелаАнотація:
Ethylene response factors (ERFs) are involved in biotic and abiotic stress; however, the drought resistance mechanisms of many ERFs in soybeans have not been resolved. Previously, we proved that GmERF113 enhances resistance to the pathogen Phytophthora sojae in soybean. Here, we determined that GmERF113 is induced by 20% PEG-6000. Compared to the wild-type plants, soybean plants overexpressing GmERF113 (GmERF113-OE) displayed increased drought tolerance which was characterized by milder leaf wilting, less water loss from detached leaves, smaller stomatal aperture, lower Malondialdehyde (MDA) content, increased proline accumulation, and higher Superoxide dismutase (SOD) and Peroxidase (POD) activities under drought stress, whereas plants with GmERF113 silenced through RNA interference were the opposite. Chromatin immunoprecipitation and dual effector-reporter assays showed that GmERF113 binds to the GCC-box in the GmPR10-1 promoter, activating GmPR10-1 expression directly. Overexpressing GmPR10-1 improved drought resistance in the composite soybean plants with transgenic hairy roots. RNA-seq analysis revealed that GmERF113 downregulates abscisic acid 8′-hydroxylase 3 (GmABA8’-OH 3) and upregulates various drought-related genes. Overexpressing GmERF113 and GmPR10-1 increased the abscisic acid (ABA) content and reduced the expression of GmABA8’-OH3 in transgenic soybean plants and hairy roots, respectively. These results reveal that the GmERF113-GmPR10-1 pathway improves drought resistance and affects the ABA content in soybean, providing a theoretical basis for the molecular breeding of drought-tolerant soybean.
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Thu, Nguyen Binh Anh, Quang Thien Nguyen, Xuan Lan Thi Hoang, Nguyen Phuong Thao, and Lam-Son Phan Tran. "Evaluation of Drought Tolerance of the Vietnamese Soybean Cultivars Provides Potential Resources for Soybean Production and Genetic Engineering." BioMed Research International 2014 (2014): 1–9. http://dx.doi.org/10.1155/2014/809736.
Повний текст джерелаАнотація:
Drought is one of the greatest constraints to soybean production in many countries, including Vietnam. Although a wide variety of the newly produced cultivars have been produced recently in Vietnam through classical breeding to cope with water shortage, little knowledge of their molecular and physiological responses to drought has been discovered. This study was conducted to quickly evaluate drought tolerance of thirteen local soybean cultivars for selection of the best drought-tolerant cultivars for further field test. Differences in drought tolerance of cultivars were assessed by root and shoot lengths, relative water content, and drought-tolerant index under both normal and drought conditions. Our data demonstrated that DT51 is the strongest drought-tolerant genotype among all the tested cultivars, while the highest drought-sensitive phenotype was observed with MTD720. Thus, DT51 could be subjected to further yield tests in the field prior to suggesting it for use in production. Due to their contrasting drought-tolerant phenotypes, DT51 and MTD720 provide excellent genetic resources for further studies underlying mechanisms regulating drought responses and gene discovery. Our results provide vital information to support the effort of molecular breeding and genetic engineering to improve drought tolerance of soybean.
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Chen, Zhanyu, Xiaokun Fang, Xueshun Yuan, Yingying Zhang, Huiying Li, Ying Zhou, and Xiyan Cui. "Overexpression of Transcription Factor GmTGA15 Enhances Drought Tolerance in Transgenic Soybean Hairy Roots and Arabidopsis Plants." Agronomy 11, no. 1 (January 18, 2021): 170. http://dx.doi.org/10.3390/agronomy11010170.
Повний текст джерелаАнотація:
Soybean (Glycine max) is one of the important oil crops worldwide. In recent years, environmental stresses such as drought and soil salinization have severely deteriorated soybean yield and quality. We investigated the overexpression of the transcription factor GmTGA15 in response to drought stress in transgenic soybean hairy roots and Arabidopsis plants. The results of quantitative real time polymerase chain reaction (qRT-PCR) analyses showed that GmTGA15 was greatly induced by salt, PEG6000, salicylic acid (SA), gibberellic acid (GA), abscisic acid (ABA), and methyl jasmonate (MeJA) in soybean. In response to drought stress, the contents of both chlorophyll and proline were significantly increased, while the content of malondialdehyde (MDA) was significantly decreased in the soybean hairy roots with the overexpression of GmTGA15 in comparison to wild type (WT). Under the simulated drought conditions, the transgenic Arabidopsis plants showed significantly longer roots and lower mortality than that of the wild type. These results suggest that GmTGA15 promotes tolerance to drought stress in both soybean and Arabidopsis plants. This study provides the scientific evidence for further functional analysis of soybean TGA transcription factors in drought stress and the breeding of drought-resistance crops.
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Ha, Chien Van, Dung Tien Le, Rie Nishiyama, Yasuko Watanabe, Uyen Thi Tran, Nguyen Van Dong, and Lam-Son Phan Tran. "Characterization of the Newly Developed Soybean Cultivar DT2008 in Relation to the Model Variety W82 Reveals a New Genetic Resource for Comparative and Functional Genomics for Improved Drought Tolerance." BioMed Research International 2013 (2013): 1–8. http://dx.doi.org/10.1155/2013/759657.
Повний текст джерелаАнотація:
Soybean (Glycine max) productivity is adversely affected by drought stress worldwide, including Vietnam. In the last few years, we have made a great effort in the development of drought-tolerant soybean cultivars by breeding and/or radiation-induced mutagenesis. One of the newly developed cultivars, the DT2008, showed enhanced drought tolerance and stable yield in the field conditions. The purpose of this study was to compare the drought-tolerant phenotype of DT2008 and Williams 82 (W82) by assessing their water loss and growth rate under dehydration and/or drought stress conditions as a means to provide genetic resources for further comparative and functional genomics. We found that DT2008 had reduced water loss under both dehydration and drought stresses in comparison with W82. The examination of root and shoot growths of DT2008 and W82 under both normal and drought conditions indicated that DT2008 maintains a better shoot and root growth rates than W82 under both two growth conditions. These results together suggest that DT2008 has better drought tolerance degree than W82. Our results open the way for further comparison of DT2008 and W82 at molecular levels by advanced omic approaches to identify mutation(s) involved in the enhancement of drought tolerance of DT2008, contributing to our understanding of drought tolerance mechanisms in soybean. Mutation(s) identified are potential candidates for genetic engineering of elite soybean varieties to improve drought tolerance and biomass.
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Fatema, Mst Kaniz, Muhammad Abdullah Al Mamun, Umakanta Sarker, Muhammad Saddam Hossain, Muhammad Abdul Baset Mia, Rajib Roychowdhury, Sezai Ercisli, Romina Alina Marc, Olubukola Oluranti Babalola, and Muhammad Abdul Karim. "Assessing Morpho-Physiological and Biochemical Markers of Soybean for Drought Tolerance Potential." Sustainability 15, no. 2 (January 11, 2023): 1427. http://dx.doi.org/10.3390/su15021427.
Повний текст джерелаАнотація:
Drought stress provokes plants to change their growth pattern and biochemical contents to overcome adverse situations. Soybean was grown under 40 (drought) and 80% (control) of field capacity (FC) to determine the morpho-physiological and biochemical alterations that occur under drought conditions. The experiment was conducted following a randomized complete block design with three replications. The results showed that drought exerted detrimental effects on photosynthetic attributes, leaf production, pigment and water content, plant growth, and dry matter production of soybean. However, drought favored producing a higher amount of proline and malondialdehyde in soybean leaf than in the control. The pod and seed production, grain size, and seed yield of soybean were also adversely affected by the drought, where genotypic variations were conspicuous. Interestingly, the studied morpho-physiological and biochemical parameters of AGS383 were minimally affected by drought. This genotype was capable of maintaining healthier root and shoot growth, greater leaf area, preserving leaf greenness and cell membrane stability, higher photosynthesis, absorbing water and sustaining leaf water potential, and lower amount of proline and malondialdehyde production under drought conditions. The heavier grains of AGS383 make it out yielder under both growth conditions. Considering the changes in morpho-physiological, biochemical, and yield contributing parameters, the genotype AGS383 could be cultivated as a relatively drought-tolerant, high-yielding soybean variety. Further study is needed to uncover the genes responsible for the adaptation of AGS383 to drought-stress environments, and this genotype might be used as parent material in a breeding program to develop a high-yielding, drought-tolerant soybean variety.
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Avksentiieva, Olga, and Nataliia Taran. "DROUGHT RESISTANCE AND PRODUCTIVITY OF WHEAT AND SOYBEAN ISOGENIC LINES WITH DIFFERENT PHOTOPERIODIC SENSITIVITY." EUREKA: Life Sciences 5 (September 30, 2016): 8–17. http://dx.doi.org/10.21303/2504-5695.2016.00226.
Повний текст джерелаАнотація:
The results of the study of drought tolerance of isogenic (NILs – near isogenic lines) by genes PPD (photoperiod) wheat lines and isogenic by genes ЕЕ (early maturation) soybean lines, that control the photoperiodic sensitivity are presented. In field experiments the photoperiodic sensitivity of the lines when grown under natural long days (16 hours at a latitude of Kharkov) and under artificial short-day (9 hours) is determined. The results showed that line PРD-D1A and PPD-A1a wheat and soybean lines L 71-920 had a weak photoperiodic sensitivity (weak PPDS) and line PPD-B1a wheat and soybean lines L 71-920 - strong photoperiodic sensitivity (strong PPDS). Wheat and soybean lines with weak PPDS were more productive. When simulating drought action on seed germination (20% strength mannitol solution - rapid method), it was showed that the seeds of soybean and wheat lines with weak PPDS have a higher germination than seeds of the lines with strong PPDS. When simulating soil drought (30% FMC – field moisture capacity of the soil) under growing experiment, it was revealed that the biomass accumulation indices of plants, leaf relative water content (RWC) and proline content in leaves lines with weak PPDS were higher than in the photoperiodic lines with strong PPDS. So, all used methods for determining drought tolerance showed that the low photoperiodic sensitivity lines are more resistant to drought. It is assumed that wheat genes PPD and soybean genes EE can participate in the formation of resistance to drought. Genotypes with low photoperiodic sensitivity should be used in breeding soybean and wheat drought resistance.
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Dhungana, Sanjeev Kumar, Ji-Hee Park, Jae-Hyeon Oh, Beom-Kyu Kang, Jeong-Hyun Seo, Jung-Sook Sung, Hong-Sik Kim, Sang-Ouk Shin, In-Youl Baek, and Chan-Sik Jung. "Quantitative Trait Locus Mapping for Drought Tolerance in Soybean Recombinant Inbred Line Population." Plants 10, no. 9 (August 31, 2021): 1816. http://dx.doi.org/10.3390/plants10091816.
Повний текст джерелаАнотація:
Improving drought stress tolerance of soybean could be an effective way to minimize the yield reduction in the drought prevailing regions. Identification of drought tolerance-related quantitative trait loci (QTLs) is useful to facilitate the development of stress-tolerant varieties. This study aimed to identify the QTLs for drought tolerance in soybean using a recombinant inbred line (RIL) population developed from the cross between a drought-tolerant ‘PI416937’ and a susceptible ‘Cheonsang’ cultivar. Phenotyping was done with a weighted drought coefficient derived from the vegetative and reproductive traits. The genetic map was constructed using 2648 polymorphic SNP markers that distributed on 20 chromosomes with a mean genetic distance of 1.36 cM between markers. A total of 10 QTLs with 3.52–4.7 logarithm of odds value accounting for up to 12.9% phenotypic variance were identified on seven chromosomes. Five chromosomes—2, 7, 10, 14, and 20—contained one QTL each, and chromosomes 1 and 19 harbored two and three QTLs, respectively. The chromosomal locations of seven QTLs overlapped or located close to the related QTLs and/or potential candidate genes reported earlier. The QTLs and closely linked markers could be utilized in maker-assisted selection to accelerate the breeding for drought tolerance in soybean.
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Patriyawaty, Nia Romania, and Gatut W. Anggara. "Pertumbuhan dan hasil genotipe kedelai (Glycine max (L.) Merril) pada tiga tingkat cekaman kekeringan." AGROMIX 11, no. 2 (July 26, 2020): 151–65. http://dx.doi.org/10.35891/agx.v11i2.2024.
Повний текст джерелаАнотація:
Drought stress is a major constraint to the production and yield stability of soybean (Glycine max (L.) Merr). Drought stress at the generative phase had a negative impact on soybean potential yield significantly. This study evaluated variation in five soybean genotypes (G1, G2, G3, G4 and G5) in their response to the three levels (100% of field capacity (D1), 80% from field capacity (D2) and 60% from field capacity (D3)) of drought stress in a glass house. This study was arranged in a factorial randomized completely block design with three replications. Results showed that all five genotypes responded significantly (P<0.05) to the drought stress treatments in soybean yield. The average yield was respectively reduced by 8.4% and 11.6% in the D2 and D3 treatments, respectively, compared to the D1 (control). Percent reduction of yield for genotype G4 (19.8%) was lower compared to other genotypes. This is indicated that genotype G4 tolerance to drought stress. The evaluation on the morphological character was potentially to become a screening tool for soybean breeding program.
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Моlodchenkova, O. O., L. T. Mishchenko, T. V. Kartuzova, L. Ya Bezkrovnaya, О. B. Likhota, G. D. Lavrova, and Е. Sh Мursakaev. "Biochemical characteristics of soybean varieties under viral infection and different growth conditions." Faktori eksperimental'noi evolucii organizmiv 24 (August 30, 2019): 259–64. http://dx.doi.org/10.7124/feeo.v24.1112.
Повний текст джерелаАнотація:
Aim. The aim of the work was to investigate of particularities of biochemical composition of seed and plant vegetative mass of different soybean varieties at the action of viral infection and agroclimatic conditions. Меthods. Standard and adapted methods of biochemical analysis were used for laboratory research. Isolation of glycinin and β-conglycinin was carried out using method developed in the laboratory (Pat #42181). Results. It was established that infection by Soybean mosaic virus (SKP-16 and SGP-17 isolates) causes changes in the biochemical characteristics (content of protein, main storage protein fractions (glycinin and β-conglycinin), fat, carbohydrates, isoflavones, activity of lectin, lipoxygenase, trypsin inhibitor) in the infected seeds that depend on the soybean variety, the infection degree of the plants and the conditions of cultivation. The determination of the relative moisture content, proline content, and lectin activity in the leaves of the 2 upper layers of soybean plants, which differed on the level of drought-tolerance in the phases of flowering, bob formation and filling of beans showed that contents of all studied biochemical characteristics significantly increased in the drought-tolerant soybean varieties as compared with not drought-tolerant ones in the phase of filling of beans. Conclusions. The obtained results can be used for development of the methods of soybean varieties selection with high seed quality and complex resistance (to the cultivation conditions and viral infection) and will be recommended for implementation in breeding and agricultural practices.Keywords: soybean, plant breeding, SМV, drought, biochemical characteristics.
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Sulieman, Saad, Chien Van Ha, Maryam Nasr Esfahani, Yasuko Watanabe, Rie Nishiyama, Chung Thi Bao Pham, Dong Van Nguyen, and Lam-Son Phan Tran. "DT2008: A Promising New Genetic Resource for Improved Drought Tolerance in Soybean When Solely Dependent on Symbiotic N2Fixation." BioMed Research International 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/687213.
Повний текст джерелаАнотація:
Water deficit is one of the major constraints for soybean production in Vietnam. The soybean breeding research efforts conducted at the Agriculture Genetics Institute (AGI) of Vietnam resulted in the development of promising soybean genotypes, suitable for the drought-stressed areas in Vietnam and other countries. Such a variety, namely, DT2008, was recommended by AGI and widely used throughout the country. The aim of this work was to assess the growth of shoots, roots, and nodules of DT2008 versus Williams 82 (W82) in response to drought and subsequent rehydration in symbiotic association as a means to provide genetic resources for genomic research. Better shoot, root, and nodule growth and development were observed in the cultivar DT2008 under sufficient, water deficit, and recovery conditions. Our results represent a good foundation for further comparison of DT2008 and W82 at molecular levels using high throughput omic technologies, which will provide huge amounts of data, enabling us to understand the genetic network involved in regulation of soybean responses to water deficit and increasing the chances of developing drought-tolerant cultivars.
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Salloum, María Soraya, María Florencia Menduni, and Celina Mercedes Luna. "A differential capacity of arbuscular mycorrhizal fungal colonization under well-watered conditions and its relationship with drought stress mitigation in unimproved vs. improved soybean genotypes." Botany 96, no. 2 (February 2018): 135–44. http://dx.doi.org/10.1139/cjb-2017-0137.
Повний текст джерелаАнотація:
Modern breeding programs may cause a reduction in plant responsiveness to arbuscular mycorrhizal (AM) fungi. In this study, we tested the hypothesis that responses such as higher arbuscule formation and mycorrhizal dependency (MD) in unimproved soybean genotypes than in improved genotypes is related to drought stress tolerance caused by enhanced growth parameters and oxidative stress regulation. Firstly, four unimproved and four improved soybean genotypes were compared under well-watered conditions. After 20 days, all of the unimproved soybean genotypes showed increased arbuscule formation, as well as a positive and higher MD index in foliar mineral nutrient and growth parameters compared with the four improved genotypes. Secondly, tolerance to drought stress was evaluated in the two improved soybean genotypes and the two unimproved genotypes selected for the most contrasting response to arbuscule formation under well-watered conditions. After 20 days of 30% of field capacity, arbuscule formation was higher in the unimproved than improved genotypes. Mycorrhizal dependency evaluated as leaf area as well as shoot and root dry mass were highest in the unimproved AM genotypes. Moreover, levels of malondiadehide were lower and proline was higher in the unimproved rather than the improved genotypes. The potential capacity of arbuscule formation is discussed as a selection criterion to identify improved soybean genotypes with increased efficiency under well-watered conditions and an enhanced capacity to relieve drought stress.
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Yu, X., A. T. James, A. Yang, A. Jones, O. Mendoza-Porras, C. A. Bétrix, H. Ma, and M. L. Colgrave. "A comparative proteomic study of drought-tolerant and drought-sensitive soybean seedlings under drought stress." Crop and Pasture Science 67, no. 5 (2016): 528. http://dx.doi.org/10.1071/cp15314.
Повний текст джерелаАнотація:
Drought is a major factor limiting plant growth causing yield reduction in crops; hence the characterisation of drought tolerance and the development of drought-tolerant crop varieties have been a goal of many crop breeding programs. Using the proteomics approach, we compared the differential protein abundance of drought-tolerant and drought-sensitive soybean leaves subjected to mild or severe drought stress. Proteins were extracted and separated using two-dimensional electrophoresis. Those protein spots with significant and more than 2-fold difference in abundance, 174 in total, were further analysed and 102 proteins were positively identified. Around 38.5% of these proteins were related to energy metabolism and photosynthetic functions, followed by those associated with defence response (36.4%) and protein metabolism (25.2%). Severe drought resulted in a greater number of proteins with differential abundance. Genotypes responded differently to drought stress with the tolerant genotype showing a higher capacity for reactive oxygen species scavenging and maintaining energy supply than the sensitive genotype. The sensitive genotype had a greater number of proteins with significant differential abundance than the tolerant genotypes due to drought. The different patterns in protein abundance induced by drought stress may potentially be utilised to screen and select candidate soybean lines with improved drought tolerance.
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Ouyang, Wenqi, Limiao Chen, Junkui Ma, Xiaorong Liu, Haifeng Chen, Hongli Yang, Wei Guo, et al. "Identification of Quantitative Trait Locus and Candidate Genes for Drought Tolerance in a Soybean Recombinant Inbred Line Population." International Journal of Molecular Sciences 23, no. 18 (September 16, 2022): 10828. http://dx.doi.org/10.3390/ijms231810828.
Повний текст джерелаАнотація:
With global warming and regional decreases in precipitation, drought has become a problem worldwide. As the number of arid regions in the world is increasing, drought has become a major factor leading to significant crop yield reductions and food crises. Soybean is a crop that is relatively sensitive to drought. It is also a crop that requires more water during growth and development. The aim of this study was to identify the quantitative trait locus (QTL) that affects drought tolerance in soybean by using a recombinant inbred line (RIL) population from a cross between the drought-tolerant cultivar ‘Jindou21’ and the drought-sensitive cultivar ‘Zhongdou33’. Nine agronomic and physiological traits were identified under drought and well-watered conditions. Genetic maps were constructed with 923,420 polymorphic single nucleotide polymorphism (SNP) markers distributed on 20 chromosomes at an average genetic distance of 0.57 centimorgan (cM) between markers. A total of five QTLs with a logarithm of odds (LOD) value of 4.035–8.681 were identified on five chromosomes. Under well-watered conditions and drought-stress conditions, one QTL related to the main stem node number was located on chromosome 16, accounting for 17.177% of the phenotypic variation. Nine candidate genes for drought resistance were screened from this QTL, namely Glyma.16G036700, Glyma.16G036400, Glyma.16G036600, Glyma.16G036800, Glyma.13G312700, Glyma.13G312800, Glyma.16G042900, Glyma.16G043200, and Glyma.15G100700. These genes were annotated as NAC transport factor, GATA transport factor, and BTB/POZ-MATH proteins. This result can be used for molecular marker-assisted selection and provide a reference for breeding for drought tolerance in soybean.
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Simondi, Sebastián, Esteban Casaretto, Gastón Quero, Sergio Ceretta, Victoria Bonnecarrère, and Omar Borsani. "A Simple and Accurate Method Based on a Water-Consumption Model for Phenotyping Soybean Genotypes under Hydric Deficit Conditions." Agronomy 12, no. 3 (February 25, 2022): 575. http://dx.doi.org/10.3390/agronomy12030575.
Повний текст джерелаАнотація:
Drought limits crop productivity and reduces yield stability. Drought tolerance as a selection criterion in breeding programs requires the development of high-throughput, precise, and low-cost phenotyping strategies. We developed a mathematical model, based on biological approaches, for evaluating soybean plants’ response to drought under controlled growth conditions. The model describes the kinetics of water consumption of a plant pot substrate system (PPS) with low sampling requirements. The model generated two parameters, t0.5 (time necessary for the PPS to reach half of the maximum amount of evapotranspirable water) and Gw(t0.5) (stomatal conductance [Gw] at t0.5), which determined the water- consumption curve of each genotype. An analysis of the kinetics of water consumption in response to a progressive water deficit in a biparental and breeding population was performed as a preliminary test of the model. A correspondence analysis between the t0.5 and Gw(t0.5) parameters with the genetic structure of the populations shows a genetic association. The phenotyping methodology presented in this work and drought susceptibility in field conditions are discussed based on previous results. This work could be useful for improving the selection of soybean genotypes in relation to their performance under drought conditions.
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Rosenzweig, V. E., and D. V. Goloenko. "Breeding strategies for soybean canopy structure optimization in dry regions." Oil Crops 186, no. 2 (June 30, 2021): 24–30. http://dx.doi.org/10.25230/2412-608x-2021-2-186-24-30.
Повний текст джерелаАнотація:
Water supply is one of the key factors limiting soybean yield. Coming from the monsoon climate region, soybean lacks effective means of leaf surface growth restriction and is prone to produce excessive leaf area that leads to undesirable transpiration increase. Reducing branching rate and, correspondingly, leaf number per plant is usually proposed to decrease leaf area. However, as far as branching ability is generally a useful trait contributing to yield stability, we have undertaken a search for possible alternative ways of leaf area reduction. Soybean canopy structure was studied in our germplasm nursery in Kursk region. We have updated an express method of soybean trifoliate leaf surface calculation. A regression index for soybean trifoliate leaf surface by central leaflet length and width product characterizes leaflet shape and depends from its length to width ratio. In the sampling studied, trifoliate leaf surface varied from 79 to 150sq. cm. Leaf area index (LAI) varied from 4.0 to 8.6 sq. m/sq. m, with optimal LAI equal to 6.0 sq. m/sq. m. Excessive LAI (over 7.7 sq. m/sq. m) decreased yield by 20 %. Optimal LAI may be achieved by various combinations of leaf size and leaf number per plant. Lines possessing good branching rate but remaining within optimal LAI values due to small leaf size were revealed. Thus, lamina size reduction may be proposed as an alternative breeding direction to solve a conflict of bushy plant type and drought tolerance.
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Matoša Kočar, Maja, Ana Josipović, Aleksandra Sudarić, Tomislav Duvnjak, Marija Viljevac Vuletić, Monika Marković, and Antonela Markulj Kulundžić. "Chlorophyll a fluorescence as tool in breeding drought stress-tolerant soybean." Journal of Central European Agriculture 23, no. 2 (2022): 305–17. http://dx.doi.org/10.5513/jcea01/23.2.3437.
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Virginia, Salvador, Megan Pagan, Melissa Cooper, Stella K. Kantartzi, David A. Lightfoot, Khalid Meksem, and My Abdelmajid Kassem. "Genetic Analysis of Relative Water Content (RWC) in Two Recombinant Inbred Line Populations of Soybean [Glycine max (L.) Merr.]." Plant Genetics, Genomics, and Biotechnology 1, no. 2 (June 15, 2017): 46–53. http://dx.doi.org/10.5147/pggb.v1i2.151.
Повний текст джерелаАнотація:
Drought affects soybean [Glycine max (L.) Merr.] and other crops productivity in the US and other parts of the world. Relative water content (RWC) is an important indicator for plant water deficit tolerance (WDT). The objective of this study is to map quantitative trait loci (QTL) for RWC and several other leaf traits such as leaf dry weight (LDW), leaf fresh weight (LFW), and leaf turgid weight (LTW) in two soybean recombinant inbred line (RIL) populations, one derived from a cross of ‘Essex’ and ‘Forrest’ (ExF, n=94) and the other is derived from a cross of ‘PI 438489B’ and ‘Hamilton’ (PIxH, n=50). In the PIxH RIL population, eight QTL were identified and mapped on 6 different linkage groups (LGs) of the soybean genome. No QTL for LFW were identified in this population. In the ExF RIL population, 10 QTL were identified and mapped on 5 different LGs of soybean. Chromosome 18 (LG G) contains clusters of QTL for LFW, LTW, and RWC in the ExF RIL population. This same chromosome contains a QTL for RWC in the PIxH RIL population. The QTL found here are important to be included in breeding programs for soybean water deficit tolerance (WDT).
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Zelentsov, S. V., E. V. Moshnenko, M. V. Trunova, L. A. Bubnova, E. N. Budnikov, G. M. Saenko, and S. A. Ramazanova. "An early cold- and drought-resistant soybean variety Triada." Oil Crops 186, no. 2 (June 30, 2021): 92–97. http://dx.doi.org/10.25230/2412-608x-2021-2-186-92-97.
Повний текст джерелаАнотація:
The early maturing soybean cultivar Triada was developed as a result of individual selection in F4 from the hybrid combination Belogorskaya × Vilana on differentiating backgrounds for traits of reduced response to day length, cold and frost resistance, drought tolerance. Additionally, the degree of cold resistance of the initial germplasm for the breeding from a cultivar Iney was evaluated by a cryosedimentation method. According to the results of the variety trials of 2019–2020, the cultivar Triada in terms of yield exceeded the standard cultivar Slavia by 0.62 t/ha. The height of plants at the latitude of Krasnodar (45°) is 95–125 cm. The new high-yielding soybean cultivar Triada is highly resistant to low air and soil temperatures, and reacts weakly to nonoptimal day lengths. This allows it to be cultivated as a main crop in the Northern Caucasian, Central Chernozem and Low Volga regions of the Russian Federation, as well as in the Republics of Kazakhstan, Uzbekistan and Kyrgyzstan at geographic latitudes from 40 to 50°.
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Khan, Mueen. "Achievements and prospects of molecular breeding for drought tolerance in soybean [Glycine max (L.) merr.]." Genetika 50, no. 3 (2018): 1095–109. http://dx.doi.org/10.2298/gensr1803095k.
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Salloum, M. S., M. C. Guzzo, M. S. Velazquez, M. B. Sagadin, and C. M. Luna. "Variability in colonization of arbuscular mycorrhizal fungi and its effect on mycorrhizal dependency of improved and unimproved soybean cultivars." Canadian Journal of Microbiology 62, no. 12 (December 2016): 1034–40. http://dx.doi.org/10.1139/cjm-2016-0383.
Повний текст джерелаАнотація:
Breeding selection of germplasm under fertilized conditions may reduce the frequency of genes that promote mycorrhizal associations. This study was developed to compare variability in mycorrhizal colonization and its effect on mycorrhizal dependency (MD) in improved soybean genotypes (I-1 and I-2) with differential tolerance to drought stress, and in unimproved soybean genotypes (UI-3 and UI-4). As inoculum, a mixed native arbuscular mycorrhizal fungi (AMF) was isolated from soybean roots, showing spores mostly of the species Funneliformis mosseae. At 20 days, unimproved genotypes followed by I-2, showed an increase in arbuscule formation, but not in I-1. At 40 days, mycorrhizal plants showed an increase in nodulation, this effect being more evident in unimproved genotypes. Mycorrhizal dependency, evaluated as growth and biochemical parameters from oxidative stress was increased in unimproved and I-2 since 20 days, whereas in I-1, MD increased at 40 days. We cannot distinguish significant differences in AMF colonization and MD between unimproved and I-2. However, variability among improved genotypes was observed. Our results suggest that selection for improved soybean genotypes with good and rapid AMF colonization, particularly high arbuscule/hyphae ratio could be a useful strategy for the development of genotypes that optimize AMF contribution to cropping systems.
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Liu, Congge, Hong Wang, Yu Zhang, Haijing Cheng, Zhangli Hu, Zhen-Ming Pei, and Qing Li. "Systematic Characterization of the OSCA Family Members in Soybean and Validation of Their Functions in Osmotic Stress." International Journal of Molecular Sciences 23, no. 18 (September 12, 2022): 10570. http://dx.doi.org/10.3390/ijms231810570.
Повний текст джерелаАнотація:
Since we discovered OSCA1, a hyperosmolarity-gated calcium-permeable channel that acted as an osmosensor in Arabidopsis, the OSCA family has been identified genome-wide in several crops, but only a few OSCA members’ functions have been experimentally demonstrated. Osmotic stress seriously restricts the yield and quality of soybean. Therefore, it is essential to decipher the molecular mechanism of how soybean responds to osmotic stress. Here, we first systematically studied and experimentally demonstrated the role of OSCA family members in the osmotic sensing of soybean. Phylogenetic relationships, gene structures, protein domains and structures analysis revealed that 20 GmOSCA members were divided into four clades, of which members in the same cluster may have more similar functions. In addition, GmOSCA members in clusters III and IV may be functionally redundant and diverged from those in clusters I and II. Based on the spatiotemporal expression patterns, GmOSCA1.6, GmOSCA2.1, GmOSCA2.6, and GmOSCA4.1 were extremely low expressed or possible pseudogenes. The remaining 16 GmOSCA genes were heterologously overexpressed in an Arabidopsis osca1 mutant, to explore their functions. Subcellular localization showed that most GmOSCA members could localize to the plasma membrane (PM). Among 16 GmOSCA genes, only overexpressing GmOSCA1.1, GmOSCA1.2, GmOSCA1.3, GmOSCA1.4, and GmOSCA1.5 in cluster I could fully complement the reduced hyperosmolality-induced [Ca2+]i increase (OICI) in osca1. The expression profiles of GmOSCA genes against osmotic stress demonstrated that most GmOSCA genes, especially GmOSCA1.1, GmOSCA1.2, GmOSCA1.3, GmOSCA1.4, GmOSCA1.5, GmOSCA3.1, and GmOSCA3.2, strongly responded to osmotic stress. Moreover, overexpression of GmOSCA1.1, GmOSCA1.2, GmOSCA1.3, GmOSCA1.4, GmOSCA1.5, GmOSCA3.1, and GmOSCA3.2 rescued the drought-hypersensitive phenotype of osca1. Our findings provide important clues for further studies of GmOSCA-mediated calcium signaling in the osmotic sensing of soybean and contribute to improving soybean drought tolerance through genetic engineering and molecular breeding.
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Wang, Wubin, Bin Zhou, Jianbo He, Jinming Zhao, Cheng Liu, Xianlian Chen, Guangnan Xing, Shouyi Chen, Han Xing, and Junyi Gai. "Comprehensive Identification of Drought Tolerance QTL-Allele and Candidate Gene Systems in Chinese Cultivated Soybean Population." International Journal of Molecular Sciences 21, no. 14 (July 8, 2020): 4830. http://dx.doi.org/10.3390/ijms21144830.
Повний текст джерелаАнотація:
Drought is one of the most important factors affecting plant growth and productivity. The previous results on drought tolerance (DT) genetic system in soybean indicated a complex of genes not only few ones were involved in the trait. This study is featured with a relatively thorough identification of QTL-allele/candidate-gene system using an efficient restricted two-stage multi-locus multi-allele genome-wide association study, on two comprehensive DT indicators, membership index values of relative plant weight (MPW) and height (MPH), instead of a single biological characteristic, in a large sample (564 accessions) of the Chinese cultivated soybean population (CCSP). Based on 24,694 multi-allele markers, 75 and 64 QTL with 261 and 207 alleles (2–12/locus) were detected for MPW and MPH, explaining 54.7% and 47.1% of phenotypic variance, respectively. The detected QTL-alleles were organized into a QTL-allele matrix for each indicator, indicating DT is a super-trait conferred by two (even more) QTL-allele systems of sub-traits. Each CCSP matrix was separated into landrace (LR) and released cultivar (RC) sub-matrices, which showed significant differentiation in QTL-allele constitutions, with 58 LR alleles excluded and 16 new ones emerged in RC. Using the matrices, optimal crosses with great DT transgressive recombinants were predicted. From the detected QTL, 177 candidate genes were annotated and validated with quantitative Real-time PCR, and grouped into nine categories, with ABA and stress responders as the major parts. The key point of the above results is the establishment of relatively full QTL-allele matrices composed of numerous gene functions jointly conferring DT, therefore, demonstrates the complexity of DT genetic system and potential of CCSP in DT breeding.
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Ncube, Efficient, Keletso Mohale, and Noluyolo Nogemane. "Metabolomics as a Prospective Tool for Soybean (Glycine max) Crop Improvement." Current Issues in Molecular Biology 44, no. 9 (September 12, 2022): 4181–96. http://dx.doi.org/10.3390/cimb44090287.
Повний текст джерелаАнотація:
Global demand for soybean and its products has stimulated research into the production of novel genotypes with higher yields, greater drought and disease tolerance, and shorter growth times. Genetic research may be the most effective way to continue developing high-performing cultivars with desirable agronomic features and improved nutritional content and seed performance. Metabolomics, which predicts the metabolic marker for plant performance under stressful conditions, is rapidly gaining interest in plant breeding and has emerged as a powerful tool for driving crop improvement. The development of increasingly sensitive, automated, and high-throughput analytical technologies, paired with improved bioinformatics and other omics techniques, has paved the way for wide characterization of genetic characteristics for crop improvement. The combination of chromatography (liquid and gas-based) with mass spectrometry has also proven to be an indisputable efficient platform for metabolomic studies, notably plant metabolic fingerprinting investigations. Nevertheless, there has been significant progress in the use of nuclear magnetic resonance (NMR), capillary electrophoresis, and Fourier-transform infrared spectroscopy (FTIR), each with its own set of benefits and drawbacks. Furthermore, utilizing multivariate analysis, principal components analysis (PCA), discriminant analysis, and projection to latent structures (PLS), it is possible to identify and differentiate various groups. The researched soybean varieties may be correctly classified by using the PCA and PLS multivariate analyses. As metabolomics is an effective method for evaluating and selecting wild specimens with desirable features for the breeding of improved new cultivars, plant breeders can benefit from the identification of metabolite biomarkers and key metabolic pathways to develop new genotypes with value-added features.
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Bailey, Amanda, Kent Burkey, Matthew Taggart, and Thomas Rufty. "Leaf Traits That Contribute to Differential Ozone Response in Ozone-Tolerant and Sensitive Soybean Genotypes." Plants 8, no. 7 (July 20, 2019): 235. http://dx.doi.org/10.3390/plants8070235.
Повний текст джерелаАнотація:
Ozone (O3) is a phytotoxic air pollutant that limits crop productivity. Breeding efforts to improve yield under elevated O3 conditions will benefit from understanding the mechanisms that contribute to O3 tolerance. In this study, leaf gas exchange and antioxidant metabolites were compared in soybean genotypes (Glycine max (L.) Merr) differing in ozone sensitivity. Mandarin (Ottawa) (O3-sensitive) and Fiskeby III (O3-tolerant) plants grown under charcoal-filtered (CF) air conditions for three weeks were exposed for five days to either CF conditions or 70 ppb O3 in continuously stirred tank reactors (CSTRs) in a greenhouse. In the CF controls, stomatal conductance was approximately 36% lower for Fiskeby III relative to Mandarin (Ottawa) while the two genotypes exhibited similar levels of photosynthesis. Ozone exposure induced significant foliar injury on leaves of Mandarin (Ottawa) associated with declines in both stomatal conductance (by 77%) and photosynthesis (by 38%). In contrast, O3 exposure resulted in minimal foliar injury on leaves of Fiskeby III with only a small decline in photosynthesis (by 5%), and a further decline in stomatal conductance (by 30%). There was a general trend towards higher ascorbic acid content in leaves of Fiskeby III than in Mandarin (Ottawa) regardless of treatment. The results confirm Fiskeby III to be an O3-tolerant genotype and suggest that reduced stomatal conductance contributes to the observed O3 tolerance through limiting O3 uptake by the plant. Reduced stomatal conductance was associated with enhanced water-use efficiency, providing a potential link between O3 tolerance and drought tolerance.
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Brensha, Williams, Stella K. Kantartzi, Khalid Meksem, Robert L. Grier IV, Abdelali Barakat, David A. Lightfoot, and My Abdelmajid Kassem. "Genetic Analysis of Root and Shoot Traits in the ‘Essex’ By ‘Forrest’ Recombinant Inbred Line (RIL) Population of Soybean [Glycine max (L.) Merr.]." Plant Genetics, Genomics, and Biotechnology 1, no. 1 (June 15, 2017): 1–9. http://dx.doi.org/10.5147/pggb.v1i1.146.
Повний текст джерелаАнотація:
Crop productivity is severely reduced by water deficit and drought in many plant species including soybean. Improved root and shoot traits can contribute to drought tolerance ability of the plant. This research was conducted to identify QTL that underlie several root and shoot traits in the ‘Essex’ by ‘Forrest’ (ExF RILs, n=94) recombinant inbred line (RIL) soybean population. Field collected samples were used for gathering phenotypic data of basal root thickness (BRT), lateral root number (LRN), maximum root length (MRL), root fresh weight (RFW), root dry weight (RDW), shoot fresh weight (SFW), shoot dry weight (SDW), and calculating RFW/SFW, and RDW/SDW ratios. All traits and ratios were compared against DNA markers using the composite interval mapping (CIM). A total of 12 QTL: 3 for MRL, 1 QTL for LRN, 1 QTL for BRT, 2 QTL for RFW, 2 QTL for RDW, 4 QTL for SFW, 3 QTL for SDW, and 3 QTL for SFW/SDW were identified and mapped on different linkage groups (LGs) A2, B2, C2, D1a, F, G, and N. The LOD scores of these QTL ranged from 2.5 to 6.0. No QTL were associated with RFW/RDW. The root and shoot trait QTL of this study may benefit breeding programs for producing cultivars tolerant to water deficit and high yield. Preliminary analyses of genes the QTL regions using GO annotation gave insight into genes that may underlie some of these QTLs.
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Johana, Dea, Rosliana Purwaning Dyah, Mufidah Mufidah, Suprayogi Suprayogi, Dani Satyawan, and I. Made Tasma. "Segregation Analysis of SSR Markers and Phenotypic Performances of Four Soybean F2 Populations Based on Morphological Characters and SSR Markers." Jurnal AgroBiogen 18, no. 1 (August 12, 2022): 11. http://dx.doi.org/10.21082/jbio.v18n1.2022.p11-20.
Повний текст джерелаАнотація:
<p>The role of molecular markers in variety development is very important in increasing selection efficiency through analysis of segregation patterns for subsequent populations. The aims of this study were to analyze patterns of soybean F2 population segregation using SSR markers and morphological characters and analyze phenotypic performances of four soybean F2 populations. Each F2 genotype was evaluated molecularly and phenotypically. The SSR markers and qualitative phenotypic traits, i.e. flower, hypocotyle, and pubescent color segregation ratios, were analyzed using Chi-square tests and the normality of quantitative phenotypic data was done using Liliefors test. Results showed that SSR markers segregated in Mendelian fashion, with a ratio of 1:2:1 of codominant SSR marker, in three F2 populations (Grobogan × Introduksi 10, Grobogan × Introduksi 13, and Biosoy 1 × Introduksi 10). Meanwhile, in F2 population Grobogan × Introduksi 12 the SSR markers did not demonstrate a 1:2:1 segregation ratio. However, that population skewed toward alleles from Grobogan. Phenotypic data of flower, hypocotyle, and pubescent colors were segregated in a 3:1 ratio of dominant monogenic genes for populations Grobogan × Introduksi 13 and Biosoy 1 × Introduksi 10. Liliefors analysis showed that quantitative characters, such as plant height, pod number/plant, seed yield/plant, and 100-seed weight observed in the F2 populations showed normal distribution as expected since the characters are controlled by many genes. Three of the four F2 populations resulted from this study can be used for genetic mapping study and for breeding programs of aluminum-toxicity, flooding, and drought tolerance characters in soybean.</p>
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Pereyra-Irujo, Gustavo A., Emmanuel D. Gasco, Laura S. Peirone, and Luis A. N. Aguirrezábal. "GlyPh: a low-cost platform for phenotyping plant growth and water use." Functional Plant Biology 39, no. 11 (2012): 905. http://dx.doi.org/10.1071/fp12052.
Повний текст джерелаАнотація:
Breeding drought-tolerant crop varieties with higher water use efficiency could help maintain food supply to a growing population and save valuable water resources. Fast and accurate phenotyping is currently a bottleneck in the process towards attaining this goal, as available plant phenotyping platforms have an excessive cost for many research institutes or breeding companies. Here we describe a simple and low-cost, automatic platform for high-throughput measurement of plant water use and growth and present its utilisation to assess the drought tolerance of two soybean genotypes. The platform allows the evaluation of up to 120 plants growing in individual pots. A cart moving in only one direction carries the measuring and watering devices. Watering and measurement routines allow the simulation of multiple water regimes for each plant individually and indicate the timing of measurement of soil water content and image capture for growth estimation. Water use, growth and water use efficiency were measured in two experiments with different water scenarios. Differences in water use efficiency between genotypes were detected only in some treatments, emphasising the importance of phenotyping platforms to evaluate a genotype’s phenotype under a broad range of conditions in order to capture valuable differences, minimising the chance of artefacts and increasing precision of measurements.
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James, A. T., R. J. Lawn, and M. Cooper. "Genotypic variation for drought stress response traits in soybean. III. Broad-sense heritability of epidermal conductance, osmotic potential, and relative water content." Australian Journal of Agricultural Research 59, no. 7 (2008): 679. http://dx.doi.org/10.1071/ar07161.
Повний текст джерелаАнотація:
The broad-sense heritability of 3 traits related to leaf survival in severely stressed plants was studied in several hybrid soybean populations. The 3 traits were epidermal conductance (ge), osmotic potential (π), and relative water content (RWC). The populations were generated by hybridising unrelated parental genotypes previously shown to differ in the 3 traits. ge (mm/s) was measured on well watered plants from 10 populations involving all combinations of 5 parental lines, grown in soil-filled beds in the glasshouse. π (MPa) and RWC (%) were measured on severely stressed plants of 3 populations involving all combinations of 3 different parents, growing into a terminal water deficit under a rainout shelter in the field. Broad-sense heritability for ge was significantly different from zero (P < 0.05) in all 10 populations and ranged from 60% to 93%. Heritability estimates for π70 (the tissue osmotic potential at 70% RWC) ranged from 33% to 71%. Only two estimates were statistically significant (P < 0.05) because of large standard errors and the fact that parental differences were smaller than previously observed. Broad-sense heritability for RWC of severely stressed plants ranged from 40% to 74%, and was statistically significant (P < 0.05) for 2 of the 3 populations. For all 3 traits, F2 progeny distributions were consistent with quantitative inheritance with a high degree of additive gene action. It was concluded that capacity exists to breed varieties with low ge, low π70, and high RWC in stressed plants. However, in the case of osmotic potential, genotypes with lower π70 combined with greater precision of measurement would be needed than proved possible in these studies. Further, specific strategies would be needed to select for the critical RWC, the minimal RWC at which leaf tissues die and which provides a measure of tissue dehydration tolerance. More research is also needed to characterise the dynamic relations between ge, π, and RWC in influencing leaf survival in soybean, before they could be confidently used in a breeding program to improve drought tolerance.
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Tay Fernandez, Cassandria Geraldine, Benjamin John Nestor, Monica Furaste Danilevicz, Mitchell Gill, Jakob Petereit, Philipp Emanuel Bayer, Patrick Michael Finnegan, Jacqueline Batley, and David Edwards. "Pangenomes as a Resource to Accelerate Breeding of Under-Utilised Crop Species." International Journal of Molecular Sciences 23, no. 5 (February 28, 2022): 2671. http://dx.doi.org/10.3390/ijms23052671.
Повний текст джерелаАнотація:
Pangenomes are a rich resource to examine the genomic variation observed within a species or genera, supporting population genetics studies, with applications for the improvement of crop traits. Major crop species such as maize (Zea mays), rice (Oryza sativa), Brassica (Brassica spp.), and soybean (Glycine max) have had pangenomes constructed and released, and this has led to the discovery of valuable genes associated with disease resistance and yield components. However, pangenome data are not available for many less prominent crop species that are currently under-utilised. Despite many under-utilised species being important food sources in regional populations, the scarcity of genomic data for these species hinders their improvement. Here, we assess several under-utilised crops and review the pangenome approaches that could be used to build resources for their improvement. Many of these under-utilised crops are cultivated in arid or semi-arid environments, suggesting that novel genes related to drought tolerance may be identified and used for introgression into related major crop species. In addition, we discuss how previously collected data could be used to enrich pangenome functional analysis in genome-wide association studies (GWAS) based on studies in major crops. Considering the technological advances in genome sequencing, pangenome references for under-utilised species are becoming more obtainable, offering the opportunity to identify novel genes related to agro-morphological traits in these species.
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Sintaha, Mariz, Chun-Kuen Man, Wai-Shing Yung, Shaowei Duan, Man-Wah Li, and Hon-Ming Lam. "Drought Stress Priming Improved the Drought Tolerance of Soybean." Plants 11, no. 21 (November 2, 2022): 2954. http://dx.doi.org/10.3390/plants11212954.
Повний текст джерелаАнотація:
The capability of a plant to protect itself from stress-related damages is termed “adaptability” and the phenomenon of showing better performance in subsequent stress is termed “stress memory”. While drought is one of the most serious disasters to result from climate change, the current understanding of drought stress priming in soybean is still inadequate for effective crop improvement. To fill this gap, in this study, the drought memory response was evaluated in cultivated soybean (Glycine max). To determine if a priming stress prior to a drought stress would be beneficial to the survival of soybean, plants were divided into three treatment groups: the unprimed group receiving one cycle of stress (1S), the primed group receiving two cycles of stress (2S), and the unstressed control group not subjected to any stress (US). When compared with the unprimed plants, priming led to a reduction of drought stress index (DSI) by 3, resulting in more than 14% increase in surviving leaves, more than 13% increase in leaf water content, slight increase in shoot water content and a slower rate of loss of water from the detached leaves. Primed plants had less than 60% the transpiration rate and stomatal conductance compared to the unprimed plants, accompanied by a slight drop in photosynthesis rate, and about a 30% increase in water usage efficiency (WUE). Priming also increased the root-to-shoot ratio, potentially improving water uptake. Selected genes encoding late embryogenesis abundant (LEA) proteins and MYB, NAC and PP2C domain-containing transcription factors were shown to be highly induced in primed plants compared to the unprimed group. In conclusion, priming significantly improved the drought stress response in soybean during recurrent drought, partially through the maintenance of water status and stronger expression of stress related genes. In sum, we have identified key physiological parameters for soybean which may be used as indicators for future genetic study to identify the genetic element controlling the drought stress priming.
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Riduan, Ahmad, Rainiyati Rainiyati, Yuia Alia, and Sosiawan Nusifera. "Tolerance Some Soybean Cultivars to Stress Drought at Vegetative to Generative Phase." Jurnal Penelitian Pendidikan IPA 8, SpecialIssue (December 12, 2022): 1–11. http://dx.doi.org/10.29303/jppipa.v8ispecialissue.2487.
Повний текст джерелаАнотація:
Soybean is one of crop which many conducting in Jambi province, where this area is one of sentra soybean producer in Indonesia. Main constraint in expansion of soybean in Jambi province is ability of adaptation of low crop to condition of area that is partly are consisted of area of marjinal with level of low fertility , applies strains or cultivar indication by drought tolerance to earn is one of the solutions forplies strains or cultivar indication by drought tolerant to earn is one of alternative of trouble-shooting. Examination of soybean cultivar tolerance to stres drought at vegetative to generative phase (15 - 45 Day After Plants / DAP) done in glasshouse by using Split Plout Design. Treatment of stres drought as main check and soybean cultivar as child of check. From result of observation, treatment of stres dryness at vegetative phase and generative can reduce and pursues growth and result of soybean cultivar tested. But for soybean cultivar Derap-1, Dena-2, Deja-2, Dering-1, Grobogan, Detam-1 and Dega-1, treatment of stres drought exactly causes improvement of root length to range from 7,14 - 60,57%. Based on variable dry weigh seed,seven soybean cultivars tested canot be classified in group of tolerant and tolerant medium to stres drought at vegetative to generative phase that is var. Argo Mulyo, Dena-1, Derap-1, Dena-2, Deja-2, Dering-1, Grobogan dan Detam-1. Stres drought causes improvement can of leaf prthe mechanism of soybean tolerance to stres drought can improve root length as mechanism of tolerance in morphology and or improveprovement of root length as mechanism of tolerance in morphology and or through improvement of proline content as mechanism of tolerance physiologically
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Linh, Tran My, Nguyen Chi Mai, Pham Thi Hoe, Le Quynh Lien, Ninh Khac Ban, Le Thi Thu Hien, Nguyen Hoai Chau, and Nguyen Tuong Van. "Metal-Based Nanoparticles Enhance Drought Tolerance in Soybean." Journal of Nanomaterials 2020 (June 20, 2020): 1–13. http://dx.doi.org/10.1155/2020/4056563.
Повний текст джерелаАнотація:
Drought is a major abiotic stress that negatively impacts plant growth and crop production. Among various techniques used to alleviate drought stress in plants, nanoparticle application is considered to be effective and promising. In this study, the responses of plants treated with iron, copper, cobalt, and zinc oxide nanoparticles (NPs) were analyzed in soybean under drought-induced conditions. The obtained results indicated that these metal-based NPs supported the drought tolerance of NP-treated plants. The desired physiological traits, viz., relative water content, drought tolerance index, and biomass reduction rate, were significantly improved, especially in iron NP-treated plants. At the molecular level, quantitative PCR analysis of several drought-responsive genes revealed a gene-, tissue-, and NP-dependent upregulation of gene expression. Iron NP treatment promoted the expression of all tested genes in roots; additionally, the expression of three drought-responsive genes increased in leaves of all NP-treated plants, while the expression of GmERD1 (Early Responsive to Dehydration 1) was induced in both roots and shoots under the four NP treatments tested. Our findings suggest that NP application can improve drought tolerance of soybean plants by triggering drought-associated gene expression.
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Sun, Maolin, Yue Li, Jiqiang Zheng, Depeng Wu, Chunxia Li, Zeyang Li, Ziwei Zang, et al. "A Nuclear Factor Y-B Transcription Factor, GmNFYB17, Regulates Resistance to Drought Stress in Soybean." International Journal of Molecular Sciences 23, no. 13 (June 29, 2022): 7242. http://dx.doi.org/10.3390/ijms23137242.
Повний текст джерелаАнотація:
Soybean is sensitive to drought stress, and increasing tolerance to drought stresses is an important target for improving the performance of soybean in the field. The genetic mechanisms underlying soybean’s drought tolerance remain largely unknown. Via a genome-wide association study (GWAS) combined with linkage analysis, we identified 11 single-nucleotide polymorphisms (SNPs) and 22 quantitative trait locus (QTLs) that are significantly associated with soybean drought tolerance. One of these loci, namely qGI10-1, was co-located by GWAS and linkage mapping. The two intervals of qGI10-1 were differentiated between wild and cultivated soybean. A nuclear factor Y transcription factor, GmNFYB17, was located in one of the differentiated regions of qGI10-1 and thus selected as a candidate gene for further analyses. The analysis of 29 homologous genes of GmNFYB17 in soybean showed that most of the genes from this family were involved in drought stress. The over-expression of GmNFYB17 in soybean enhanced drought resistance and yield accumulation. The transgenic plants grew better than control under limited water conditions and showed a lower degree of leaf damage and MDA content but higher RWC, SOD activity and proline content compared with control. Moreover, the transgenic plants showed a fast-growing root system, especially regarding a higher root–top ratio and more branching roots and lateral roots. The better agronomic traits of yield were also found in GmNFYB17 transgenic plants. Thus, the GmNFYB17 gene was proven to positively regulate drought stress resistance and modulate root growth in soybean. These results provide important insights into the molecular mechanisms underlying drought tolerance in soybean.
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Wrather, J. A., J. G. Shannon, T. E. Carter, J. P. Bond, J. C. Rupe, and A. M. R. Almeida. "Reaction of Drought-Tolerant Soybean Genotypes to Macrophomina phaseolina." Plant Health Progress 9, no. 1 (January 2008): 16. http://dx.doi.org/10.1094/php-2008-0618-01-rs.
Повний текст джерелаАнотація:
Charcoal rot caused by Macrophomina phaseolina is a common disease of many crops including common bean and soybean. Incidence and severity of charcoal rot are enhanced when plants are drought stressed. Resistance to this pathogen in some common bean genotypes was associated with drought tolerance. Resistance to M. phaseolina among soybean genotypes has not been identified, although a few have been rated moderately resistant based on less root tissue colonization by this pathogen compared to other genotypes. A few soybean genotypes have been rated as slow-wilt or drought-tolerant. The reaction of drought-tolerant soybean to M. phaseolina compared to intolerant or drought-sensitive genotypes has not been determined. Our objective was to determine if there were differences in root colonization by M. phaseolina between drought-tolerant and drought-sensitive soybean genotypes. Drought tolerance of the soybean genotypes and root colonization by M. phaseolina at the R6 and R8 stages of growth were not related in this study. Some drought-tolerant soybean genotypes may resist root colonization by M. phaseolina, but our results suggest that this is not true for all drought-tolerant genotypes. Accepted for publication 21 March 2008. Published 18 June 2008.
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Yu, Qian, Ya-Li Liu, Guo-Zhong Sun, Yuan-Xia Liu, Jun Chen, Yong-Bin Zhou, Ming Chen, You-Zhi Ma, Zhao-Shi Xu, and Jin-Hao Lan. "Genome-Wide Analysis of the Soybean Calmodulin-Binding Protein 60 Family and Identification of GmCBP60A-1 Responses to Drought and Salt Stresses." International Journal of Molecular Sciences 22, no. 24 (December 16, 2021): 13501. http://dx.doi.org/10.3390/ijms222413501.
Повний текст джерелаАнотація:
Calmodulin-binding protein 60 (CBP60) members constitute a plant-specific protein family that plays an important role in plant growth and development. In the soybean genome, nineteen CBP60 members were identified and analyzed for their corresponding sequences and structures to explore their functions. Among GmCBP60A-1, which primarily locates in the cytomembrane, was significantly induced by drought and salt stresses. The overexpression of GmCBP60A-1 enhanced drought and salt tolerance in Arabidopsis, which showed better state in the germination of seeds and the root growth of seedlings. In the soybean hairy roots experiment, the overexpression of GmCBP60A-1 increased proline content, lowered water loss rate and malondialdehyde (MDA) content, all of which likely enhanced the drought and salt tolerance of soybean seedlings. Under stress conditions, drought and salt response-related genes showed significant differences in expression in hairy root soybean plants of GmCBP60A-1-overexpressing and hairy root soybean plants of RNAi. The present study identified GmCBP60A-1 as an important gene in response to salt and drought stresses based on the functional analysis of this gene and its potential underlying mechanisms in soybean stress-tolerance.
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Jin, Ting, Zhong Shan, Shuang Zhou, Qianqian Yang, Junyi Gai, and Yan Li. "GmDNAJC7 from Soybean Is Involved in Plant Tolerance to Alkaline-Salt, Salt, and Drought Stresses." Agronomy 12, no. 6 (June 13, 2022): 1419. http://dx.doi.org/10.3390/agronomy12061419.
Повний текст джерелаАнотація:
Soybean [Glycine max (L.) Merri.] is an important oilseed and food crop. In recent years, environmental degradation has accelerated soil alkalization, salinization, and water deficit, which have seriously threatened the soybean quality and yield. Chaperone DNAJ proteins play important roles in plant response to a number of abiotic and biotic stresses. Here, we investigated the function of a soybean DNAJ gene, GmDNAJC7, in plant tolerance to abiotic stresses. GmDNAJC7 gene expression was induced by alkaline-salt, salt, and drought treatments in soybean roots, suggesting its possible role in soybean response to these stresses. GmDNAJC7 overexpression improved the alkaline-salt tolerance of soybean composite plants, which showed a higher SPAD (Soil and Plant Analysis Development) value for chlorophyll content and leaf relative water content than the control plants after NaHCO3 treatment. Moreover, the GmDNAJC7 overexpressing Arabidopsis had a higher germination rate and average root length than the wild type and dnajc7 mutant, under NaHCO3, NaCl, and mannitol stresses, indicating that the ectopic expression of the GmDNAJC7 gene enhanced the alkaline-salt, salt, and drought tolerance in Arabidopsis. These findings suggest that GmDNAJC7 is involved in the alkaline-salt, salt, and drought tolerance in Arabidopsis and soybean. This study provides new insights into the role of DNAJ proteins in plant tolerance to abiotic stress.
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Moussa, H. "Low dose of gamma irradiation enhanced drought tolerance in soybean." Acta Agronomica Hungarica 59, no. 1 (March 1, 2011): 1–12. http://dx.doi.org/10.1556/aagr.59.2011.1.1.
Повний текст джерелаАнотація:
Drought stress is the main limiting factor in soybean production. However, no work has been done on how the application of a low dose of gamma rays could help to overcome water deficits during critical stages of soybean development. Gamma rays at a dose of 20 Gray (Gy) were applied to dry seeds of soybean before planting. Two levels of soil moisture (80% field capacity for well-watered control and 35% for drought-stressed treatment) were applied at pod initiation. Gamma irradiation increased biomass accumulation and seed yield in both treatments. It also increased the chlorophyll content, photosynthetic activity (14CO2 fixation) and leaf water potential and enhanced the enzyme activities of RuBPCase and PEPCase of control plants compared with drought-stressed plants. Gamma irradiation (20 Gy) increased the soluble sugars, protein and proline content and the activities of peroxidase and superoxide dismutase in drought-stressed soybean leaves. It also increased the chloroplast size, which was reduced by drought treatment, and rebuilt, to some extent, the chloroplast ultrastructure. However, it decreased the malondialdehyde concentration and the electrical conductivity of the leaves under drought stress. Overall, the results indicated that pre-treatment with gamma rays (20 Gy) to dry seeds of soybean before planting could be used to enhance drought tolerance and minimize the yield loss caused by water deficit.
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Sloane, Richard J., Robert P. Patterson, and Thomas E. Carter. "Field Drought Tolerance of a Soybean Plant Introduction." Crop Science 30, no. 1 (January 1990): 118–23. http://dx.doi.org/10.2135/cropsci1990.0011183x003000010027x.
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Sapra, Val T., and Anthony O. Anaele. "Screening Soybean Genotypes for Drought and Heat Tolerance." Journal of Agronomy and Crop Science 167, no. 2 (August 1991): 96–102. http://dx.doi.org/10.1111/j.1439-037x.1991.tb00939.x.
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Liu, Zhilei, Yuanjing Li, Lina Ma, Haichao Wei, Jianfeng Zhang, Xingyuan He, and Chunjie Tian. "Coordinated Regulation of Arbuscular Mycorrhizal Fungi and Soybean MAPK Pathway Genes Improved Mycorrhizal Soybean Drought Tolerance." Molecular Plant-Microbe Interactions® 28, no. 4 (April 2015): 408–19. http://dx.doi.org/10.1094/mpmi-09-14-0251-r.
Повний текст джерелаАнотація:
Mitogen-activated protein kinase (MAPK) cascades play important roles in the stress response in both plants and microorganisms. The mycorrhizal symbiosis established between arbuscular mycorrhizal fungi (AMF) and plants can enhance plant drought tolerance, which might be closely related to the fungal MAPK response and the molecular dialogue between fungal and soybean MAPK cascades. To verify the above hypothesis, germinal Glomus intraradices (syn. Rhizophagus irregularis) spores and potted experiments were conducted. The results showed that AMF GiMAPKs with high homology with MAPKs from Saccharomyces cerevisiae had different gene expression patterns under different conditions (nitrogen starvation, abscisic acid treatment, and drought). Drought stress upregulated the levels of fungi and soybean MAPK transcripts in mycorrhizal soybean roots, indicating the possibility of a molecular dialogue between the two symbiotic sides of symbiosis and suggesting that they might cooperate to regulate the mycorrhizal soybean drought-stress response. Meanwhile, the changes in hydrogen peroxide, soluble sugar, and proline levels in mycorrhizal soybean as well as in the accelerated exchange of carbon and nitrogen in the symbionts were contributable to drought adaptation of the host plants. Thus, it can be preliminarily inferred that the interactions of MAPK signals on both sides, symbiotic fungus and plant, might regulate the response of symbiosis and, thus, improve the resistance of mycorrhizal soybean to drought stress.
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Yadav, Riya, Diptimayee Dash, and T. Chowdhury. "Evaluation of promising soybean rhizobia for conferring drought tolerance in soybean under pot." International Journal of Chemical Studies 8, no. 4 (July 1, 2020): 3505–9. http://dx.doi.org/10.22271/chemi.2020.v8.i4ar.10194.
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Suryaningrum, Ratih, Edi Purwanto, and Sumiyati Sumiyati. "Analisis Pertumbuhan Beberapa Varietas Kedelai pada Perbedaan Intensitas Cekaman Kekeringan." Agrosains: Jurnal Penelitian Agronomi 18, no. 2 (September 12, 2016): 33. http://dx.doi.org/10.20961/agsjpa.v18i2.18686.
Повний текст джерелаАнотація:
<p>Soybean demand in the market today has not been matched by domestic production. Many of the problems that cause insufficient production of soy, one of which is the drought affecting soybean growth and yield. Efforts should be made to overcome the drought is to provide soybean varieties tolerant to drought stress. This study aims to assess the effect of the difference in intensity of drought stress on the growth of some soybean varieties. This study uses a completely randomized design (CRD) factorial with two factors, the first factor and the second factor soybean varieties drought stress levels. Results showed significant of lowering the value of RGR and NAR. The higher the level of drought stress will decrease soybean growth. Tolerance limits for each variety is at 80% moisture content.</p>
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Sarkar, KK, MA Mannan, MM Haque, and JU Ahmed. "Physiological Basis of Water Stress Tolerance in Soybean." Bangladesh Agronomy Journal 18, no. 2 (July 25, 2016): 71–78. http://dx.doi.org/10.3329/baj.v18i2.28908.
Повний текст джерелаАнотація:
An experiment was conducted to study the effects of water stress on physiological parameters associated to drought tolerance in soybean at the Department of Agronomy, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh during January to April, 2015. Four soybean genotypes namely i) BU Soybean 1 ii) Binasoybean 1 iii) Galarsum and iv) BARI Soybean 5 were grown in two watering regimes viz. control (80% of the field capacity) and water stress (50% of the field capacity). Genotypic variability was found in water stress tolerance in soybean. Highest accumulation of leaf proline, sugar and water content and lower accumulation of malondialdehyde were found in Binasoybean 1 compared to other genotypes. Lowest yield reduction was found in Binasoybean 1. Binasoybean 1 showed relatively higher drought tolerance whereas BARI Soybean 5 was found susceptible to yield. It was found that higher water stress tolerance in Binasoybean 1 was associated with better water relations and higher accumulation of sugar and proline and lower accumulation of malondialdehyde content in leaf.Bangladesh Agron. J. 2015, 18(2): 71-78
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Cao, D., Y. L. Yan, and D. H. Xu. "Assessment of salt tolerance and analysis of the salt tolerance gene Ncl in Indonesian soybean germplasm." Plant Genetic Resources: Characterization and Utilization 17, no. 03 (January 15, 2019): 265–71. http://dx.doi.org/10.1017/s1479262118000618.
Повний текст джерелаАнотація:
AbstractSoybean [Glycine max (L.) Merr.] is one of the most important legume crops in the world. However, soybean grain yield is extensively affected by environmental stresses such as soil salinity. In this study, we evaluated the germplasm of 51 Indonesian soybean accessions for salt tolerance to identify salt-tolerant germplasms for possible use in breeding for soybean salt tolerance. Based on experiments under hydroponic conditions, adding 100 mM of NaCl to a 1/2 concentration of Hoagland and Arnon solution, several Indonesian soybean germplasms, such as Java 7, Seputih Raman, Tambora, Ringgit (JP 30217), Sinyonya (early) and Sinyonya (late) were identified as salt-tolerant in terms of salt tolerance rate (STR) and leaf chlorophyll content (SPAD value) taken with the Konica Minolta SPAD-502 chlorophyll meter. The selected salt-tolerant germplasms were further evaluated under soil medium cultivation in pots irrigated with 100 mM NaCl for around 5 weeks. The six selected soybean germplasms again showed higher salt tolerance in terms of SPAD, STR and shoot dry weight. Expression analysis of the salt tolerance gene Ncl revealed a significant positive correlation between Ncl expression and salt tolerance, suggesting that Ncl is essential for salt tolerance in the Indonesian soybean germplasms we tested. The salt-tolerant Indonesian soybean germplasms identified in this study could be used in local soybean breeding practices for the improvement of salt tolerance.
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Pham, Thi Thanh Nhan, Huu Quan Nguyen, Thi Ngoc Lan Nguyen, Xuan Tan Dao, Danh Thuong Sy, Van Son Le, and Hoang Mau Chu. "Overexpression of the GmDREB2 gene increases proline accumulation and tolerance to drought stress in soybean plants." March 2020, no. 14(03):2020 (March 20, 2020): 495–503. http://dx.doi.org/10.21475/ajcs.20.14.03.p2173.
Повний текст джерелаАнотація:
The dehydration responsive element binding (DREB) is a plant protein subfamily expressed when soybean plants face abiotic stresses. These DREB proteins are also considered to activate the transcription of drought-resistant genes. In this study, we present the determined results of relationships between overexpression of Glycine max DREB2 (GmDREB2) with the transcription level of Glycine max pyrroline-5-carboxylate synthetase (GmP5CS) gene, proline accumulation and drought tolerant ability transgenic soybean plants as the basis for selection of transgenic lines with high drought tolerance. GmDREB2 was inserted into a plant transgenic vector and the 35S-GmDREB2-cmyc construct was transferred into the soybean plants by Agrobacterium-mediated transformation. Recombinant GmDREB2 protein with a molecular weight of approximately 20 kDa was expressed in four transgenic soybean lines in the T1 generation. The GmP5CS gene was shown to have significantly (P<0.05) increased expression in the T2 transgenic soybean lines and higher than compared to non-transgenic plants with considering both in well watered condition and stressed conditions were from 1.06 to 1.31-fold and 1.37 to 1.95-fold, respectively. The proline content of four transgenic soybean lines increased 155.81% to 187.42 % after five days in drought-stress conditions and increased from 180.52 % to 233.74 % after nine days under drought-stress conditions (P<0.05). Therefore, the overexpression of GmDREB2 resulted in increasing transcription level of P5CS gene, proline accumulation and drought-stress tolerance of the transgenic soybean plants. The GmDREB2 transformation into soybean plants was confirmed by the results of genetically modified lines in the T2 generation (T2-1, T2-6, T2-7, and T2-8) with higher drought tolerant ability than those of non-transgenic plants.
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Oya, Tetsuji, Alexandre Lima Nepomuceno, Norman Neumaier, José Renato Bouças Farias, Satoshi Tobita, and Osamu Ito. "Drought Tolerance Characteristics of Brazilian Soybean Cultivars— Evaluation and characterization of drought tolerance of various Brazilian soybean cultivars in the field —." Plant Production Science 7, no. 2 (January 2004): 129–37. http://dx.doi.org/10.1626/pps.7.129.
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