Дисертації з теми "Single organ"
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Kolb, Kellie Elizabeth. "Single-cell response to perturbations across biological scales : single organ, organ system and phenotypic individuals." Thesis, Massachusetts Institute of Technology, 2019. https://hdl.handle.net/1721.1/123245.
Повний текст джерелаCataloged from PDF version of thesis.
Includes bibliographical references.
The biological processes that sustain a complex organism require the orchestrated dynamics of complex cellular ensembles. Several vital systems - such as the immune system, the digestive system and more - must process internal and external signals to maintain functional homeostasis in response to perturbations at the systems-level. To further understand how groups of cells collectively respond to perturbations, we have applied single-cell RNA-sequencing and complementary techniques to explore cellular behaviors within complex systems at multiple relevant biological scales: from within a single organ, to an organ system, to across several human individuals with differing genetic backgrounds linked by a shared phenotype. More specifically, at the level of the organ, we have explored acute injury responses in the liver. We have identified and described a new compensatory phase of the liver response to injury, in which surviving hepatocytes upregulate their expression of critical liver function genes to maintain overall organ function. Next, we extended our approach from a focus on an acute injury targeting a single organ to exploring chronic damage resulting from a long-term high fat diet across multiple gastrointestinal and immune compartments. Our analysis revealed molecular pathways and changes in stem gene expression which may contribute to obesity-related disease. Finally, we characterized shared features across multiple unique human donors with a common phenotype, elite control of HIV-1. We identified and validated a subset of highly functional dendritic cells, and developed broadly applicable computational approaches to identify reproducible responses across donors and to nominate candidate targets for rationally modulating the system. Overall, our work demonstrates the utility of single-cell RNA-sequencing for uncovering important cellular phenotypes that inform systems-level responses at any biological scale.
by Kellie Elizabeth Kolb.
Ph. D.
Ph.D. Massachusetts Institute of Technology, Department of Chemistry
Miller, Zachary Dalton. "Tensile Properties of Single Vaginal Smooth Muscle Cells." Thesis, Virginia Tech, 2018. http://hdl.handle.net/10919/83567.
Повний текст джерелаMaster of Science
Thair, Simone. "The discovery of functional single nucleotide polymorphisms and novel gene mechanisms that may explain mortality and organ dysfunction in septic shock." Thesis, University of British Columbia, 2013. http://hdl.handle.net/2429/45337.
Повний текст джерелаSingh, Sanjay. "Monomeric organo-aluminum and gallium monohydroxides as precursor for homo- and heterobimetallic oxides synthetic, reactivity and structural investigations including gold(I) N-heterocyclic carbene complexes /." [S.l.] : [s.n.], 2006. http://webdoc.sub.gwdg.de/diss/2006/singh.
Повний текст джерелаBoll, Hanne [Verfasser]. "Ultraschnelle hochauflösende single breath-stop-Mikro-Computertomographie thorakaler und abdomineller Organe der lebenden Maus unter Verwendung einer vereinfachten Intubationsmethode / Hanne Boll." Gießen : Universitätsbibliothek, 2012. http://d-nb.info/1064024343/34.
Повний текст джерелаKrenkel, Martin Verfasser], Tim [Akademischer Betreuer] [Salditt, and Detlev [Akademischer Betreuer] Schild. "Cone-beam x-ray phase-contrast tomography for the observation of single cells in whole organs / Martin Krenkel. Betreuer: Tim Salditt. Gutachter: Tim Salditt ; Detlev Schild." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2015. http://d-nb.info/1078774285/34.
Повний текст джерелаKrenkel, Martin [Verfasser], Tim [Akademischer Betreuer] Salditt, and Detlev [Akademischer Betreuer] Schild. "Cone-beam x-ray phase-contrast tomography for the observation of single cells in whole organs / Martin Krenkel. Betreuer: Tim Salditt. Gutachter: Tim Salditt ; Detlev Schild." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2015. http://nbn-resolving.de/urn:nbn:de:gbv:7-11858/00-1735-0000-0023-9684-C-9.
Повний текст джерелаPapenburg, Jens Gerrit. "Hörgeräte." Doctoral thesis, Humboldt-Universität zu Berlin, Philosophische Fakultät III, 2012. http://dx.doi.org/10.18452/16485.
Повний текст джерелаThe devices by which music is listened to in the age of technological (re-)production have changed over and over again. These devices must be imperceptible to the ear. Only then can music be heard. Nonetheless – this is the claim of the thesis – these devices organize hearing and are agents of a cultivation and technization of perception. Based on two case studies from the history of rock and pop music, this thesis reveals how such devices constitute not only the listener but also the music which is listened to. Through listening technologies new listening practices emerge and the corporality of the listener is newly defined. The adaptation of sound to specific listening technologies can be analysed during the mastering process, the last step in technological music production. The devices by which music is listened to are thus neither simple technologies of reproduction nor mere electrotechnical artefacts. Rather, they are assemblages of sound, corporality, and technology. In this thesis these assemblages are called “Hörgeräte” (listening devices). The listening devices of rock and pop music target – like medical-technical “Hörgeräte” (hearing aids) – the materiality of perception. Contrary to medical technologies, however, listening devices do not function as prostheses, which are calibrated according to medical industry standards. Instead, they contain excesses – of seriality and repetition – and escalations – of amplitude and high and low frequencies. The thesis is arranged in three chapters. In the first chapter I situate the main argument of the thesis within discourses of musicology, media and cultural studies, and develop my own terminology. Chapters two and three deal with case studies. In chapter two I investigate jukebox listening in 1950s rock’n’roll culture, whilst in chapter three I explore sound system listening in disco and club culture from the 1970s to the 1990s. The terminology developed in chapter one enables a comparison of the case studies.
DiFelice, Ronald Attilio. "An Investigation of Plasma Pretreatments and Plasma Polymerized Thin Films for Titanium/Polyimide Adhesion." Diss., Virginia Tech, 2001. http://hdl.handle.net/10919/27348.
Повний текст джерелаPh. D.
QIU, NAN-JIN, and 邱南津. "Determination of organ volume by single-photon emission computed tomography." Thesis, 1992. http://ndltd.ncl.edu.tw/handle/52522389536836941951.
Повний текст джерелаTsay, Ching-Chung, and 蔡慶宗. "A Study on the Single Governmental Organ for the Anti-Corruption in Taiwan." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/32752936098521954922.
Повний текст джерела國立臺灣海洋大學
海洋法律研究所
96
Corruption is the biggest barrier for the development of a country and the main reason of causing party transition in the government as well. Taiwan’s annual rating of corruptive image made by “Transparency International ” has been gradually drawn back from the 25th place in 1995 to the 34th place in 2007. Which indicates that domestic corruption is serious. There are domestic institutions in charge of anti-corruption, such as The Control Yuan, The Procuratorate, Investigation Bureau in Ministry of Justice, Department of Government Employee Ethics in Ministry of Justice. Why did they fail to bring about the desired effect? Referring to efficient results of anti-corruption in Hong Kong, Singapore and Japan, we found reasons. Besides, the determination from the authority, reformation and promotion of Investigation Bureau, which is mainly responsible for anti-corruption, are strongly needed. By integrating Investigation Bureau, Department of Government Employee Ethics and Central Office in Ministry of Justice, “Investigation Incorruption Administration” is established under the Executive Yuan with independent financial budget and as a second-class institution with guarantee term of office for the director. With higher position in the government, the anti-corruption institute could integrate resources, avoid interfering from other departments and investigate corruptions of executive officers in order to meet the goal that the inferiors imitate the superiors. In order to establish an independent commission against corruption, laws implemented for anti-corruption should be reviewed. Under the spirit of the human rights and justice, staff in Independent Commission Against Corruption is given power to investigate, prevent and execute anti-corruption work and eventually to build up an incorrupt and developed country.
Decoutere, Lies [Verfasser]. "Analysis of the association between interleukin-10 plasma levels and the association of single and multiple organ failure following severe multiple trauma / vorgelegt von: Lies Decoutere." 2004. http://d-nb.info/984388788/34.
Повний текст джерелаChan, Ken Yee. "Engineered Viral Vectors and Developed Tissue Clearing Methods for Single-cell Phenotyping in Whole Organs." Thesis, 2017. https://thesis.library.caltech.edu/10226/23/Chan-Ken-Thesis-05-31-2017.pdf.
Повний текст джерелаA central question in biology is how different cell types interact with each other and their native environment to form complex functional systems and networks. Although our ability to investigate this question has considerably expanded from the development of genetically encoded tools, some limitations still persist. For instance, we are limited in our ability to visualize the native three dimensional environments of whole organs. Additionally, it is challenging to efficiently deliver transgenes into difficult-to-target areas through direct-injections, such as the cardiac ganglia, or broadly distributed networks, such as the myenteric nervous system, which limits our ability to extensively study these areas. Therefore, tools and methods that overcome these limitations are needed. Towards this end, my thesis work has been focused on developing tools for single-cell resolution phenotyping in whole organs. I have been developing tissue clearing technologies to render whole organs transparent for optical interrogation and characterizing viral capsids and engineering viral vectors for noninvasive widespread gene delivery to the central and peripheral nervous system.
Tissue clearing techniques for three dimensional optical interrogation were invented over a century ago. However, these earlier methods used harsh organic chemicals and failed to retain the tissue’s native fluorescence or epitopes. These earlier methods eventually became incompatible to the hundreds of newly generated transgenic mouse lines that allowed for cell type-specific expression of fluorescent transgenes or to fluorescent labeling techniques, such as immunohistochemistry (IHC). The first part of my dissertation is aimed at addressing these limitations by further developing and standardizing a tissue clearing method that utilizes the vasculature to perfuse clearing reagents. This technique, called perfusion assisted agent release in situ (PARS) enables (i) whole organ clearing of soft tissue, (ii) preservation of native fluorescence, and (iii) preservation of epitopes compatible with IHC.
Although PARS allows us to optically investigate whole soft tissue organs, it is unsuitable for clearing bone tissue. The clearing of bone is important as it may provide optical access to delicate environments, such as the lymphatic vessels lining the dural sinuses beneath the skull that would otherwise be damaged through traditional methods. However, clearing bone tissue is challenging since it is composed of both soft (bone marrow) and hard (mineral) tissue. To overcome this challenge, I developed a clearing method that rendered intact bone tissue transparent by using EDTA to decalcify bones and by constructing a convective flow chamber to efficiently clear bones. This method, called Bone CLARITY, is able to preserve native fluorescence and epitopes. In order to demonstrate the utility of Bone CLARITY, I collaborated with colleagues to quantitatively access a rare and non-uniformly distributed population of osteoprogenitor cells in their native three dimensional environment. Bone CLARITY in conjunction with light-sheet microscope enabled the early detection of an increase to this osteoprogenitor population after administration of a novel anabolic drug, which may have been undetected with traditional techniques.
Towards my second goal, I have been working on characterizing adeno-associated viruses (AAVs) for non-invasive widespread gene delivery across the central or peripheral nervous system. Through systemic delivery, these novel AAVs are able to efficiently deliver transgenes to (i) difficult-to-target areas, such as the dorsal root ganglia; (ii) cellular populations that are widely distributed across the mouse body, such as neurons in the myenteric nervous system, and (iii) through highly selective barriers, such as the blood-brain barrier. These viruses enable rapid expression of transgenes for perturbing and monitoring cellular circuits, or for potentially treating neurological diseases. In addition, I worked on engineering or validating several different gene regulatory elements to achieve cell type restricted expression in transgenic and non-transgenic animals with AAVs. These viral vectors may prove useful in rapidly testing newly developed genetic tools. Finally, I developed and characterized two different two-component viral vector systems to control the density of labeling when systemically delivering genes with our novel engineered viruses. I utilized this two-component system to perform single-cell morphology studies in the CNS and PNS. Collectively, these capsids and vectors expand the AAV toolbox and enable efficient and versatile gene delivery into the CNS and PNS of transgenic and non-transgenic animals.
Lieske, Steven P. "Mechanisms underlying the production of multiple respiratory patterns by a single neural network in vitro /." 2002. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pqdiss&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft_dat=xri:pqdiss:3060233.
Повний текст джерелаKrenkel, Martin. "Cone-beam x-ray phase-contrast tomography for the observation of single cells in whole organs." Doctoral thesis, 2015. http://hdl.handle.net/11858/00-1735-0000-0023-9684-C.
Повний текст джерелаNkosi, Duduzile. "Electrochemical and electrocatalytic properties of self-assembled single-walled carbon nanotube/organo-iron hybrid systems on gold electrodes." Thesis, 2010. http://hdl.handle.net/2263/25247.
Повний текст джерелаThesis (PhD)--University of Pretoria, 2010.
Chemistry
unrestricted
Skácelík, David. "Dohledová činnost České národní banky." Doctoral thesis, 2014. http://www.nusl.cz/ntk/nusl-338429.
Повний текст джерела