Дисертації з теми "Sigme proteins"
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся з топ-50 дисертацій для дослідження на тему "Sigme proteins".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Переглядайте дисертації для різних дисциплін та оформлюйте правильно вашу бібліографію.
Sharma, Khushbu. "Studying sigma family of transcription factors in mycobacteria: In-silico and functional analyses of conserved sigma proteins of mip by in-vitro and in-vivo methods." Thesis, IIT Delhi, 2019. http://eprint.iitd.ac.in:80//handle/2074/8065.
Повний текст джерелаLee, S. F. K. "Understanding protein tyrosine phosphatase sigma function : dimer formation and interacting proteins." Thesis, University College London (University of London), 2007. http://discovery.ucl.ac.uk/1444958/.
Повний текст джерелаDonà, Valentina. "Caratterizzazione dei fattori sigma micobatterici SigE e SigF Caratterizzazione del dominio PPE della proteina PPE17 di Mycobacterium tuberculosis." Doctoral thesis, Università degli studi di Padova, 2010. http://hdl.handle.net/11577/3422362.
Повний текст джерелаRiassunto Mycobacterium tuberculosis (MTB) è l’agente eziologico della tubercolosi, patologia che nel mondo causa ogni anno due milioni di morti, con un’incidenza drammatica specie nei Paesi in via di sviluppo. Per poter trovare nuove strategie farmacologiche e vaccinali contro MTB è di fondamentale importanza lo studio dei meccanismi, che permettono la sua sopravvivenza ai vari stress ambientali, ai quali è sottoposto durante il periodo di infezione e latenza nei macrofagi dell’ospite. La fine regolazione della trascrizione di geni specifici in risposta a condizioni di stress e la peculiare struttura della sua parete giocano in merito un ruolo fondamentale. Nella prima parte del progetto di dottorato sono stati caratterizzati due fattori di trascrizione sigma micobatterici, SigE e SigF, che regolano la trascrizione di geni specifici in risposta a vari tipi di stress ambientali, come lo stress di superficie, lo stress ossidativo, il pH alcalino e lo shock termico. Anzitutto è stata studiata la regolazione trascrizionale, traduzionale e posttraduzionale del fattore di trascrizione con funzione extracitoplasmatica (ECF) SigE. Per quanto riguarda lo studio della regolazione trascrizionale, è stato possibile confermare tramite esperimenti di 5’RACE PCR e RT-PCR la presenza di tre promotori di sigE, e a dosare, a seconda delle condizioni ambientali di crescita batterica, il contributo di ciascun promotore nella trascrizione di questo gene. Dato che l’inizio della trascrizione di uno di questi promotori è sito 63 paia di basi a valle del codone di start annotato nel genoma, si è aperta l’ipotesi dell’esistenza di due isoforme di SigE. Mediante fusioni traduzionali tra specifiche sequenze di sigE con lacZ, private del proprio codone di inizio della traduzione, e successive mutagenesi sito-specifiche, è stato possibile confermare, in base all’attività beta-galattosidasica rilevata, l’esistenza di due codoni di start alternativi, un ATC ed un TTG, che codificano per un’isoforma di rispettivamente 218 e 215 di amminoacidi, oltre all’ATG già annotato nel genoma di MTB, che codifica per un’isoforma di 257 amminoacidi. Infine è stato possibile confermare, che il gene a valle di sigE codifica per il fattore anti-sigma di SigE, denominato RseA, in grado di legare entrambe le isoforme di SigE. In un secondo progetto è stato studiato anche il ruolo del fattore SigF di M. smegmatis nella biosintesi di pigmenti carotenoidi, nella resistenza a perossido d’idrogeno e nell’efficienza di trasformazione batterica. Tramite RT-PCR è stato dimostrato che SigF controlla la trascrizione di geni coinvolti nella biosintesi dei pigmenti carotenoidi, e, partendo dal presupposto che essi fungono da protezione contro i radicali liberi, è stato verificato che il mutante per il gene sigF è effettivamente più sensibile rispetto al ceppo selvatico al trattamento con perossido d’idrogeno. Infine è stato dimostrato anche, che il ceppo mutante possiede una maggiore efficienza di trasformazione rispetto al ceppo selvatico, indicando che SigF regola probabilmente la trascrizione di geni coinvolti nella permeabilità della parete. Nella seconda parte del progetto è stata caratterizzata la localizzazione della proteina PPE17 sulla superficie micobatterica. Come altri membri della famiglia PPE, la PPE17 presenta un dominio N-terminale altamente conservato, il quale, in base a diverse evidenze in letteratura, si suppone svolgere un ruolo importante per la loro traslocazione in superficie. Inoltre, si è voluto verificare un’eventuale influenza della presenza della proteina PE11 nel processo di traslocazione in o nella stabilità della PPE17, in quanto la sequenza codificante la PE11 è in tandem e co-trascritta con quella codificante la PPE17, e vi è un’interazione specifica tra queste due proteine. I dati ottenuti mediante saggi di sensibilità alla proteinasi K su ceppi di M. smegmatis, esprimenti la PPE17 intera o solo il suo dominio PPE (dPPE17) fuse all’epitopo HA, confermano che la PPE17 intera sia esposta in superficie, sia in presenza che in assenza di PE11. In base ai dati ottenuti si è infine tentato di veicolare un antigene modello (Mpt64) di MTB sulla superficie del ceppo vaccinale M. bovis BCG fondendolo con il dPPE17. Saggi di sensibilità alla proteinasi K e ELISA su cellule intere effettuati su culture di M. bovis BCG esprimenti questa proteina chimerica indicano, che essa sia effettivamente localizzata a livello superficiale. Allo stesso modo sono state costruite due ulteriori fusioni con il dPPE17 per esprimere sulla superficie micobatterica l’antigene multimerico Ag85-ESAT6 di MTB e l’antigene Csp C3 di Plasmodium berghii. In base a saggi di sensibilità alla proteinasi K svolti su ceppi di M. smegmatis esprimenti le due fusioni anche in questo caso entrambe localizzano in superficie. I ceppi di M. bovis BCG esprimenti questi antigeni sulla loro superficie saranno testati in futuro nel modello del topo per misurare un eventuale aumento della protezione rispetto al ceppo selvatico.
Morikawa, Kazuya. "Regulation of Plastid Gene Transcription by Sigma Factors and Sigma Factor Binding Proteins." Kyoto University, 2001. http://hdl.handle.net/2433/150743.
Повний текст джерела0048
新制・課程博士
博士(人間・環境学)
甲第9028号
人博第121号
12||123(吉田南総合図書館)
新制||人||30(附属図書館)
UT51-2001-F358
京都大学大学院人間・環境学研究科文化・地域環境学専攻
(主査)教授 豊島 喜則, 教授 藤堂 剛, 助教授 瀬戸口 浩彰
学位規則第4条第1項該当
Chadsey, Meggen Shepherd. "Regulation of the flagellar specific sigma factor, sigma28, of Salmonella typhimurium by the anti-sigma factor FlgM /." Thesis, Connect to this title online; UW restricted, 1998. http://hdl.handle.net/1773/11490.
Повний текст джерелаRuan, Qingguo. "Aire regulates central and peripheral tolerance through direct control of autoantigens and other key genes in thymus epithelial cells and dendritic cells." [Gainesville, Fla.] : University of Florida, 2004. http://purl.fcla.edu/fcla/etd/UFE0006464.
Повний текст джерелаTypescript. Title from title page of source document. Document formatted into pages; contains 100 pages. Includes Vita. Includes bibliographical references.
Yeung, Shu-wai. "Analysis of 14-3-3 [sigma] protein in nasopharyngeal tissues." Click to view the E-thesis via HKUTO, 2003. http://sunzi.lib.hku.hk/hkuto/record/B31971398.
Повний текст джерелаSmillie, David Andrew. "Genes encoding sigma cross-reacting proteins of Escherichia coli." Thesis, University of Edinburgh, 1994. http://hdl.handle.net/1842/14435.
Повний текст джерела楊舒瑋 and Shu-wai Yeung. "Analysis of 14-3-3 [sigma] protein in nasopharyngeal tissues." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B31971398.
Повний текст джерелаChagnon, Mélanie J. 1977. "Physiological and molecular functions of the murine receptor protein tyrosine phosphatase sigma (RPTP[sigma])." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=115661.
Повний текст джерелаFerguson, Anna Louise. "Interactions of bacterial sigma subunits with core RNA polymerase." Thesis, University of York, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.341839.
Повний текст джерелаReeves, Adam J. "Signaling and interaction of the Bacillus subtilis physical stress pathway regulators of sigma B : a dissertation /." San Antonio : UTHSC, 2007. http://proquest.umi.com/pqdweb?did=1390290691&sid=1&Fmt=2&clientId=70986&RQT=309&VName=PQD.
Повний текст джерелаLee, Chung-Sheng Brian. "Studies of SpoIIAA, the anti-anti-#sigma#'F factor of Bacillus subtilis." Thesis, University of Oxford, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.365810.
Повний текст джерелаRynda, Agnieszka. "Low dose tolerance vaccine platform, reovirus protein sigma 1 and treatment of autoimmunity." Thesis, Montana State University, 2008. http://etd.lib.montana.edu/etd/2008/rynda/RyndaA0808.pdf.
Повний текст джерелаMason, S. "Studies on the cell-binding properties of the retrovirus #sigma#1 protein." Thesis, University of Warwick, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.357840.
Повний текст джерелаLaskos, Lina 1973. "Characterisation of alternative sigma factors and the heat shock rsponse in Neisseria gonorrhoeae." Monash University, Dept. of Microbiology, 2003. http://arrow.monash.edu.au/hdl/1959.1/5617.
Повний текст джерелаXu, Rong. "The effects of structural modifications on sigma receptor binding." Diss., Columbia, Mo. : University of Missouri-Columbia, 2007. http://hdl.handle.net/10355/4742.
Повний текст джерелаThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on December 18, 2007) Vita. Includes bibliographical references.
Gilmore, Ross. "Sigma 1 protein of reovirus as a model system for the study of protein folding within the mammalian cell cytosol." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ38469.pdf.
Повний текст джерелаThibault, Stéphanie. "Biochemical and genetic approaches to understanding the functions of the protein tyrosine phosphatase-sigma." Thesis, McGill University, 2003. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=79146.
Повний текст джерелаCunning, Christofer Lee. "Regulation of the synthesis and protein stability of the alternative sigma factor RpoS in Salmonella typhimurium." Morgantown, W. Va. : [West Virginia University Libraries], 1999. http://etd.wvu.edu/templates/showETD.cfm?recnum=533.
Повний текст джерелаAmbler, Linda. "Investigation of the potential use of reovirus sigma 1 proteins for drug delivery across the gastrointestinal tract." Thesis, Open University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.278430.
Повний текст джерелаThompson, Katherine M. "Receptor protein tyrosine phosphatase sigma (RPTPơ) inhibits axonal regeneration and the rate of axon extension." Thesis, McGill University, 2002. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=29479.
Повний текст джерелаPolychronidou, Maria. "Studying the role of dimerization in regulating the activity of Receptor Protein Tyrosine Phosphatase Sigma." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=99198.
Повний текст джерелаPerrody, Elsa. "The viral protein Rki : an atypical cochaperone partner of Hsp70." Toulouse 3, 2012. http://thesesups.ups-tlse.fr/1870/.
Повний текст джерелаThe highly conserved molecular chaperone Hsp70 is involved in a plethora of cellular processes associated with protein folding. To function as a molecular chaperone, Hsp70 requires the presence of its obligate J-domain cochaperone partners (JDP). These proteins, thanks to their J-domain signature, stimulate Hsp70's ATPase activity, facilitate substrate delivery and confer specific cellular localization to Hsp70. Genome analysis of the T4-like enterobacteriophage RB43, revealed a gene encoding a putative JDP (orfan 057w). In this work, we first show that the J-domain of this JDP, named Rki, is functional in vivo. Moreover, we show that Rki specifically interacts with the E. Coli host multifunctional Hsp70/DnaK chaperone. However, in sharp contrast with the three known host JDP cochaperones of DnaK encoded by E. Coli, Rki full-length does not act as a generic cochaperone in vivo or in vitro. Expression of Rki alone is highly toxic for wild-type E. Coli, but toxicity is abolished in the absence of endogenous DnaK or when the conserved J-domain of Rki is mutated. Further in vivo analyses revealed that Rki is expressed early after infection by RB43 and that deletion of the rki gene significantly impairs RB43 proliferation. Furthermore, we show that mutations in the host dnaK gene efficiently suppress the growth phenotype of the RB43 rki deletion mutant, thus indicating that Rki specifically interferes with DnaK cellular function. Finally, we show that the interaction of Rki with the host DnaK chaperone rapidly results in the stabilization of the heat-shock factor s32, which is normally targeted for degradation by DnaK
Herrera, Yelenis. "Modulation of ASIC1a Function by Sigma-1 Receptors: Physiological and Pathophysiological Implications." [Tampa, Fla.] : University of South Florida, 2009. http://digital.lib.usf.edu/?e14.2855.
Повний текст джерелаRogers, Angela. "The role of the sigma subunit of adaptor protein-2, AP2σ2, in the regulation of calcium homeostasis". Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:743b6eb9-ddcd-46bd-aee0-78ff4b8b6b2c.
Повний текст джерелаBortoluzzi, Alessio. "Structural characterization of Mycobacterium tuberculosis RNA polymerase binding protein A (RbpA) and its interactions with sigma factors." Thesis, University of Leicester, 2013. http://hdl.handle.net/2381/28401.
Повний текст джерелаCrottès, David. "Rôle du récepteur Sigma-1 sur la régulation des canaux ioniques impliqués dans la carcinogenèse." Thesis, Nice, 2014. http://www.theses.fr/2014NICE4032/document.
Повний текст джерелаThe sigma-1 receptor is a chaperone protein active in damaged tissues. The sigma-1 receptor is mainly expressed into brain and have a neuroprotective role in ischemia and neurodegenerative diseases. The sigma-1 receptor is also expressed into cancer cell lines and recent investigations suggest its involvement into proliferation and apoptosis. However, its role in carcinogenesis remains to delineating. Ion channels are involved in numerous physiological processes (heart beating, nervous influx, …). These membrane proteins currently emerge as a new class of therapeutic targets in cancer. During my thesis, I observed that the sigma-1 receptor regulates voltage-dependent potassium channel hERG and voltage-dependent sodium channel Nav1.5 activities respectively into leukemic and breast cancer cell lines. I also demonstrated that the sigma-1 receptor, through its action on hERG channel, increases leukemia invasiveness by promoting interaction with tumor microenvironment. These results highlight the role of the sigma-1 receptor on cancer cell electrical plasticity and suggest this chaperone protein as a potential therapeutic target to limit tumor progression
Howles, Sarah Anne. "The role of the G-protein subunit, G-α-11, and the adaptor protein 2 sigma subunit, ap2-σ-2, in the regulation of calcium homeostasis". Thesis, University of Oxford, 2015. http://ora.ox.ac.uk/objects/uuid:9be3d447-4f5c-4782-8d6a-4cb25a8ac6d8.
Повний текст джерелаLang, Bradley Thomas. "THE ROLE OF PTPs IN REGENERATION FAILURE FOLLOWING SPINAL CORD INJURY." Case Western Reserve University School of Graduate Studies / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=case1417619755.
Повний текст джерелаRayner, Lucy Jane. "Exploring inhibition of the bacterial enhancer binding protein PspF and transcription factor sigma 54 using peptide-based chemical genetic approaches." Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/10957.
Повний текст джерелаStanga, Daniela. "The role of LAR RPTPs family Receptor Protein Tyrosine Phosphatases RPTP Sigma, RPTP Delta and RPTP Lar in mouse embryonic development." Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=117158.
Повний текст джерелаRPTP_Sigma, RPTP_Delta et RPTP_Lar sont trois membres de la famille des LAR_RPTPs. Ces membres partagent un patron d'expression dans différents tissus et des structures similaires. Malgré le fait qu'ils sont des acteurs important de différentes fonctions cellulaires, leur rôle dans le développement embryonnaire reste mal compris. Nos résultats montrent que RPTP_Sigma, RPTP_Delta et RPTP_Lar agissent de façon redondante dans le cœur et le foie. Les embryons triple mutants (SDLKO) commencent à présenter une létalité à partir E12.5 et un défaut dans la maturation des érythrocytes se traduisant par un délai d'énucléation des érythroblastes. De plus, ces embryons montrent un truncus arteriosus persistant causant un défaut dans la séparation entre l'aorte et l'artère pulmonaire.
Reiß, Kerstin [Verfasser], and Thilo [Akademischer Betreuer] Stehle. "Structural Analyses of the T1L Reovirus Attachment Protein sigma 1 and the Phytophthora sojae Transglutaminase GP42 / Kerstin Reiß ; Betreuer: Thilo Stehle." Tübingen : Universitätsbibliothek Tübingen, 2013. http://d-nb.info/1163396796/34.
Повний текст джерелаTran, Amanda P. "Modulation of Receptor Protein Tyrosine Phosphatase Sigma Enhances Protease Activity to Relieve Chondroitin Sulfate Proteoglycan Inhibition of Peripheral Axons and Oligodendrocytes." Case Western Reserve University School of Graduate Studies / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=case1528450936899252.
Повний текст джерелаGris, Ormo Georgia. "Selective blockade of the sigma-1 receptor for the treatment of pain of different aetiology: Preclinical studies." Doctoral thesis, Universitat de Barcelona, 2015. http://hdl.handle.net/10803/361398.
Повний текст джерелаLa presente Tesis Doctoral se centra en el estudio del receptor sigma-1 (σ1) en el campo del dolor. Esta investigación ha sido parte de un proyecto de la empresa farmacéutica ESTEVE centrado en el descubrimiento de fármacos con afinidad por el receptor σ1 para el tratamiento de dolor de diferente etiología. El objetivo principal de esta Tesis fue explorar el interés terapéutico del bloqueo del receptor σ1 para el manejo farmacológico del dolor neuropático, inflamatorio y postoperatorio. Se evaluó la potencia y eficacia del antagonista selectivo del receptor σ1, S1RA (E-52862) en estos diferentes tipos de dolor, y se comparó con otros fármacos analgésicos comercializados. Con este fin, se emplearon dos especies (rata y ratón), diferentes evaluaciones comportamentales relacionadas con el dolor (respuesta de retirada de la pata trasera a la estimulación térmica y mecánica), y diferentes estrategias farmacológicas (administración sistémica aguda y repetida del antagonista E-52862). También se utilizaron ratones knockout por el receptor σ1 para estudiar la especificidad in vivo del E-52862 y la participación del receptor σ1 en la modulación espinal de varios marcadores moleculares relacionados con el dolor con el fin de determinar el mecanismo de acción del receptor. En resumen, los resultados de esta Tesis Doctoral proporcionan nuevos conocimientos sobre el receptor σ1 y apoyan el desarrollo clínico de antagonistas selectivos por este receptor como una intervención terapéutica adecuada para lograr analgesia en condiciones de dolor de diferente etiología.
Ibrahim, Mahmoud Arafat Abd el-hamid. "Developments and applications in computer-aided drug discovery." Thesis, University of Manchester, 2012. https://www.research.manchester.ac.uk/portal/en/theses/developments-and-applications-in-computeraided-drug-discovery(eb57dde8-6190-4ea6-8fa8-219693788daf).html.
Повний текст джерелаChen, Yuemnmu. "Characterization of the sigma receptor : a cocain binding protein /." 2001. http://www.library.wisc.edu/databases/connect/dissertations.html.
Повний текст джерелаWang, Ta-Yuan, and 王大元. "characterization of extracytoplasmic function sigma factor (mab4459c) and sigma-associated protein (mab4454c) in Mycobacterium abscessus CS1c." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/46q472.
Повний текст джерела國立陽明大學
微生物及免疫學研究所
97
Mycobacterium abscessus is an increasingly important Gram-positive bacterium that can cause a wide spectrum of human infections, such as lung, skin, soft tissue and limbs infections. In earlier study by Thomas, it had been observed that Mycobacterium abscessus clinical strain 390 can be isolated as smooth and rough colony. Both of morphologies can reverse during serial passages. The spontaneously morphologic reversion rate was about 10-5~10-6 and the rough colony was more virulent than the smooth colony. In earlier study by our laboratory, we also analyzed the colony morphology variation of clinical strain CS1c. The CS1c colony morphology can switch from smooth transparent (SmT) to rough (R1), from rough (R1) to smooth opaque (SmO), and from smooth opaque (SmO) to rough (RR2) again. The CS1c morphologic reversion rate was about 10-4~10-5. The major difference between smooth and rough morphology was glycopeptidolipid (GPL) content. GPL was synthesized and regulated by GPL locus. It was predicted that two genes in GPL locus, ecf (mab_4459c) and sap (mab_4454c) may regulate GPL synthesis. The ecf that is thought as extracytoplasmic function sigma factor, may express in optimal stress conditions and growth phase, then transcribed other genes. The sap that is thought as sigma-associated protein may participate in initiation step of transcription. In this study, we demonstrated that Ecf can interact with Sap and that exogenous Ecf can enhance the transcription of ecf, sap and pks promoter but inhibit the transcription of mmpS4 promoter. In the presence of Sap, the transcriptional function of Ecf may be inhibited. In order to analyze the mechanism of colony morphology variation, we over-express exogenous Ecf in Mycobacterium abscessus R1, and found the rough colony could not switch to smooth one. Ecf and Sap protein can regulate parts of GPL locus without being involved in morphological switch. It was known that extracytoplasmic function sigma factor can be up-regulated in optimal growth condition and stress stimulation. In this study transcription from the ecf promoter can be enhanced in log phase in Mycobacterium smegmatis, whereas the optimal stress conditions remained investigated.
Lo, Yin-Hsiu, and 羅尹秀. "Characterization of extracytoplasmic function sigma factor (MSMEG_0405) and sigma-associated protein (MSMEG_0404) in Mycobacterium smegmatis MC2155." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/16218034266672715711.
Повний текст джерела國立陽明大學
微生物及免疫學研究所
98
M. smegmatis as Mycobacterium tuberculosis belongs to the family Mycobacteriaceae. It is generally considered a non-pathogenic microorganism. Therefore, Mycobacterium smegmatis is useful in laboratory experiments for the research analysis of other Mycobacteria species. Glycopeptidolipids(GPLs)are major components present on the outer layers of the cell walls of mycobacteria. Current research has focused on the role of GPL in colony morphology, sliding motility, biofilm formation, and virulence. Two genes in GPL locus, ecf (MSMEG_0405)and sap (MSMEG_0404)may regulate GPL synthesis. The ecf is predicted as an extracytoplasmic function sigma factor and the sap is predicted as a sigma–associated protein. Both may participate in the initiation step of transcription. In this study, we used bacterial two-hybrid assay to demonstrate that Ecf can interact with Sap. In addition, we generated the ecf and ecf sap knockout strain and the reporter construct of ecf, sap, mmpS4, pks, mmpS4, mbtH, fadE5, gtf2 and gtf3 promoter to analyze the promoter activity of these genes in knockout strain and wide-type. According to the previous study, activation of extracytoplasmic function sigma factor is only inducteed under optimal condition or stress stimulation. Here we employed two conditions, 0.02% SDS and 0.5M NaCl for analysis and no significant differences between the ecf or ecf sap deletion strain and the wide-type strain were observed. The previous studies showed that Lsr2 can regulate the genes in the GPL locus. We generated the lsr2 and lsr2 ecf deletion strain to analyze the promoter acitivity in ∆lsr2 knockout strain. The expression level of mbtH, gtf3, rv0926, ecf can be down-regulated; however, the expression level of fadE5, gtf2 and mmpS4 can be up-regulated in ∆lsr2 strain. In this study we demonstrate that Ecf can interact with Sap and the expression of ecf was drived by two promoters. Whether the expression of GPL genes can be regulated by Ecf and Sap remain unknown. The stress to induce Ecf expression and whether Ecf participated in Lsr2 regulation needed to be futher investigation.
Guglielmi, Kristen Marie. "Structure-function analysis of mammalian orthoreovirus attachment protein [sigma]1." Diss., 2008. http://etd.library.vanderbilt.edu/ETD-db/available/etd-03242008-110238/.
Повний текст джерелаGopal, Krishan. "Structural And Biophysical Analysis Of The Regulatory Mechanism Of Mycobacterium Tuberculosis Sigma Factors." Thesis, 2009. https://etd.iisc.ac.in/handle/2005/958.
Повний текст джерелаGopal, Krishan. "Structural And Biophysical Analysis Of The Regulatory Mechanism Of Mycobacterium Tuberculosis Sigma Factors." Thesis, 2009. http://hdl.handle.net/2005/958.
Повний текст джерелаDvořáková, Pavla. "Vlastnosti expresních vektorů pro Corynebacterium glutamicum a jejich využití při studiu faktorů sigma RNA polymerasy." Master's thesis, 2017. http://www.nusl.cz/ntk/nusl-355666.
Повний текст джерелаJané-Valbuena, Judit. "Analysis of the structural and dsRNA binding activities of reovirus protein [sigma]3." 2002. http://www.library.wisc.edu/databases/connect/dissertations.html.
Повний текст джерелаWang, Shu-Mei, and 王淑美. "The effects of nucleoid-associated proteins on the core-independent promoter interaction of Bacillus subtilis sigma-A." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/61609577386935207595.
Повний текст джерела國立中興大學
生物化學研究所
103
The nucleoid-associated proteins (NAPs) possess DNA-binding activity and the ability to alter DNA molecule by bending, wrapping, and bridging. In addition, NAPs are capable of regulating transcription in a positive or negative manner. It has been reported that the Escherichia coli H-NS protein belongs to the family of NAPs and can improve the functions of some specific regulator proteins. Recently, it has been reported that H-NS can facilitate specific DNA-binding by RNA polymerase σ70 in AT-rich gene regulatory regions. Our lab also found that E. coli H-NS seems to be able to facilitate the binding of Bacillus subtilis σA and E. coli σ70 to their cognate promoter DNA as analyzed by Electrophoretic Mobility Shift Assay (EMSA). The present study is aimed to confirm the previous EMSA results and to answer whether the B. subtilis NAPs such as DnaD, HBsu and LrpC are able to assist σA binding to its cognate promoter DNA in core-independent manner as analyzed by DNA-affinity assay. To fulfill this goal, plasmids which are able to overexpress the three NAPs were first constructed and all of them were purified by column chromatography after overexpression in E. coli BL21 (DE3). The purified NAPs were confirmed to be correct by MALDI-TOF Mass Spectrometry. Furthermore, the results of DNA-affinity assays show that LrpC seems to be able to facilitate the binding of B. subtilis σA to the G3b promoter DNA, but H-NS and HBsu are not.
Clarke, Marcie B. "The functional characterization of the quorum sensing E. coli regulators B and C in EHEC." 2005. http://edissertations.library.swmed.edu/pdf/ClarkeM121905/ClarkeMarcie.pdf.
Повний текст джерелаHe, Cheng. "14-3-3 [sigma] is a p37 AUF1 binding protein that facilitates AUF1-mediated AU-rich mRNA decay /." 2006. http://wwwlib.umi.com/dissertations/gateway.
Повний текст джерелаTypescript. Includes bibliographical references (leaves 112-127). Also available in electronic format on the World Wide Web. Access restricted to users affiliated with the licensed institutions.
Hsu, Shaoyen, and 許紹彥. "Improve solubility and activity of a recombinant enzyme protein by co-expressing sigma 32 factor in Escherichia coli." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/31355460428258494537.
Повний текст джерела國立中正大學
化學工程研究所
100
We used Escherichia coli BL21 overexpressing recombinant enzyme protein, N-acetyl-D-neuraminic acid aldolase (GST-Neu5Ac aldolase-5R or Neu5Ac aldolase), this enzyme protein overexpressed with glutathione S-transferase (GST, Glutathione S-transferase) and polyionic peptide (5R, 5 Arginine) tags became two tags fusion enzyme protein. By genetic engineering technique, we add a new cutting site into the existed E. coli strain (E. coli BL21 pGEX-2TK nana5R), to become E. coli BL21 pGEX-2TK nana5R-XhoI (abbr. XhoI). At the same time we add σ32 factor gene (rpoH) into pGEX-2TK with shine-dalgrno ribosome binding site, right after Neu5Ac aldolase sequence, to become E. coli BL21 pGEX-2TK nana5R-rpoH (abbr. rpoH). During E. coli culture, we add Isopropyl β-D-1-thiogalactopyranoside (IPTG) or arabinose to induce these engineered enzymatic protein expression. Compare with E. coli BL21 pGEX-2TK nana5R and pBAD33 rpoH (abbr. dual, control group), we studied for the changes of proteomics and the activity or solubility of target enzymatic protein. We add rpoH gene to overexpressing σ32 factor, that offsets the conditions of heat-shock. In this condition, E. coli will express heat-shock proteins to improve the defects of low solubility and activity of overexpressed proteins while inclusion body forming. This way will reduce the cost of artificial protein production. Then we extract the protein of E. coli of each group. Through 2-dimensional SDS-PAGE to study the proteomics changes of heat-shock proteins and σ32 factor. The results indicated that XhoI, rpoH and dual groups showed a significant increase of ibpA/B after 3 hours of IPTG or arabinose induction compare with control group (p < 0.05). And a significant decrease of ibpA/B protein in rpoH group compare with XhoI group after 3 hours of IPTG induction (p < 0.05). In western blot, that also indicated σ32 protein increased 3.5 times, 2.1 times and 2.1times in XhoI group after 1, 2, 3 hours of IPTG induction compare with 0 hour. And σ32 protein increased 12.6 times, 11 times and 12.8times in dual group after 1, 2, 3 hours of IPTG and arabinose induction compare with 0 hour. In rpoH group, σ32 protein increased 15.3 times, 16.4 times and 19.1 times after 1, 2, 3 hours of IPTG or arabinose induction compare with 0 hour. The solubility of Neu5AC aldolase had a significant 27% increase in rpoH group compare to XhoI group after 1 hour of IPTG induction, but decrease with induction time. The solubility of Neu5AC aldolase had a significant 10% increase in dual group compare to XhoI group after 1 hour of IPTG and arabinose induction, but decrease with induction time. Another results showed that whole cell activity in rpoH group increased 1.6 times, 1.42 times and 1.41 times after 1, 2, 3 hours of IPTG induction compare with XhoI group. And the whole cell activity in dual group also increased 3.23 times, 2.63 times and 2.29 times after 1, 2, 3 hours of IPTG and arabinose induction compare with XhoI group. The specific activity of supernatant after lysis in rpoH group increased 1.6 times, 3 times and 2.5 times after 1, 2, 3 hours of IPTG induction compare with XhoI group. And the specific activity of dual group also increased 2 times, 1.5 times and 1.7 times after 1, 2, 3 hours of IPTG and arabinose induction compare with XhoI group. The specific activity in the rest insoluble in rpoH group increased 1.4 times 1.8 times and 2.4 times after 1, 2, 3 hours of IPTG induction compare with XhoI group. And in dual group also increased 5 times, 4 times and 2.7 times after 1, 2, 3 hours of IPTG and arabinose induction compare with XhoI group.
Tsodikov, Oleg Vyacheslav. "Novel quantitative approaches to studies of protein-DNA interactions lac repressor-lac operator and E[sigma]⁷⁰ RNA polymerase-[lambdal]PR promoter complex formation /." 1998. http://catalog.hathitrust.org/api/volumes/oclc/41131217.html.
Повний текст джерелаKirchner, Eva [Verfasser]. "Structural and functional studies of the reovirus attachment protein σ1 [sigma-1] and its interaction with the receptor JAM-A = Strukturelle und funktionelle Studien am Zelladsorptionsprotein-σ1 [Zelladsorptionsprotein-sigma-1] des Reovirus und seiner Interaktion mit dem Rezeptor JAM-A / vorgelegt von Eva Kirchner". 2009. http://d-nb.info/994687494/34.
Повний текст джерела