Дисертації: "SEZ6"

1

Pigoni, Martina [Verfasser], and Stefan [Akademischer Betreuer] Lichtenthaler. "Function and diagnostic potential of the SEZ6 protein family : novel substrates of the Alzheimer protease BACE1 / Martina Pigoni ; Betreuer: Stefan Lichtenthaler." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2019. http://d-nb.info/1222436523/34.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

2

Meng, Xianchuan. "Production structure of shenzhen SEZ /." Title page, abstract and contents only, 1991. http://web4.library.adelaide.edu.au/theses/09ECM/09ecmm544.pdf.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

3

Rossi, Eliandra Mirlei. "Estudo de biossurfactante produzido pelo patógeno alimentar Salmonella Enteritidis SE86." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2015. http://hdl.handle.net/10183/133195.

Повний текст джерела

Анотація:

Salmonella Enteritidis SE86 tem sido a principal responsável por surtos de salmonelose no Rio Grande do Sul, desde 1999. Essa bactéria apresenta várias características que a diferencia de outros sorovares de Salmonella, dentre elas a capacidade de produzir biossurfactante. Porém, a caracterização desse composto e o motivo pelo qual ele é produzido ainda não foram investigados. Assim, esse estudo teve como objetivo caracterizar o biossurfactante produzido por S. Enteriditis SE86 e verificar algumas de suas prováveis funções, como por exemplo, a influência desse composto na aderência e na resistência a desinfetantes, em folhas de alface. A produção de biossurfactante por S. Enteriditis SE86 foi avaliada em caldo infusão cérebro e coração (BHI), em meio mínimo e em folhas de alface. Foram realizados testes para caracterização do composto e S. Enteritidis SE86, com e sem biossurfactante, foi inoculada em folhas de alface, a fim de avaliar a influência do biossurfactante na aderência e resistência da bactéria a diversos métodos de desinfecção de vegetais folhosos. Os resultados demonstraram que o maior índice de emulsificação (IE24: 62,95% após 72 horas) foi produzido em caldo BHI, mas a bactéria também foi capaz de produzir biossurfactante em meio mínimo (IE24: 46% após 46 h) e em contato com as folhas de alface (IE24: 52,15% após 120h). Os testes de caracterização do demonstraram que o biossurfactante é um composto polimérico que apresenta estabilidade em diferentes pH, temperatura e salinidade. O biossurfactante aumentou a aderência de S. Enteritidis SE86 (4,1 LogUFC/cm2 para 7,3 Log UFC/cm2 após 60 minutos) e contribuiu para o aumento da resistência do patógeno na superfície das folhas de alface para todos os sanitizantes testados.
Salmonella Enteritidis SE86 has been recognized for outbreaks of salmonellosis in Rio Grande do Sul-RS since 1999. This bacteria shows several characteristics that differ it from other serovars, as its ability to produce biosurfactant. However, the characterization of this compound and the reason why its produced have not been investigated yet. The present study aimed to characterize the biosurfactant produced by S. Enteritidis SE86 and to check some of their probable functions, as checking the influence of this compound in the adherence and resistance of food pathogen on lettuce leaves. The biosurfactant production by S. Enteritidis SE86 was evaluated in Brain Heart Infusion broth (BHI), in minimal medium and on lettuce leaves. Several tests were taken to characterize the compound produced and S. Enteritidis SE86 with and without biosurfactant, was inoculated on lettuce leaves to evaluate the influence of biosurfactant in adherence and resistance to several bacterial disinfection methods of leafy vegetables. The results showed that the more emulsification index (IE24: 62.95% after 72 hours) was produced in BHI broth, but S.Enteritidis SE86 was also able to produced biosurfactants in minimal medium (IE24: 46% after 46 hours) and on lettuce leaves (IE24: 52.15% after 120 hours). The characterization tests showed that the biosurfactant is a polymeric compound which is stable at different pH, temperature and salinity. The biosurfactant increased adherence of S. Enteritidis SE86 (4.1 LogUFC / cm2 to 7.3 log CFU / cm2 after 60 minutes) and contributed to increasing pathogen resistance on surface of lettuce leaves for all tested sanitizers.

Стилі APA, Harvard, Vancouver, ISO та ін.

4

Giacalone, Gabriele. "Filogenesi delle specie di allium della sez. codonoprasum." Doctoral thesis, Università di Catania, 2012. http://hdl.handle.net/10761/1057.

Повний текст джерела

Анотація:

Lo scopo di questo lavoro è uno studio filogenetico delle specie di Allium appartenenti alla sez. Codonoprasum basato sulla comparazione di dati morfologici e molecolari (nrDNA e cpDNA). I principali dati morfologici sono stati analizzati tramite il software NTSYSpc usando l analisi UPGMA. Il lavoro è stato condotto analizzando 3 differenti markers genetici che normalmente vengono usati nelle analisi filogenetiche: ITS, sbA-trnH e rbcL. Sono stati analizzati 95 campioni,provenienti da diverse stazioni dell area mediterranea, attraverso estrazione, amplificazione e sequenziamenti del materiale genetico. Le sequenze geniche ottenute sono state processato tramite il software Paup 4.0 usando il criterio della massima parsimonia. Gli alberi filogenetici ottenuti sono stati commentati e comparati con le attuali classificazioni del gen. Allium sez. Codonopraum. Da questo studio emergono nuove e interessanti relazioni filogenetiche tra le specie di Allium della sez. Codonoprasum

Стилі APA, Harvard, Vancouver, ISO та ін.

5

Dubuke, Michelle L. "The Exocyst Subunit Sec6 Interacts with Assembled Exocytic Snare Complexes: A Dissertation." eScholarship@UMMS, 2015. https://escholarship.umassmed.edu/gsbs_diss/868.

Повний текст джерела

Анотація:

In eukaryotic cells, membrane-bound vesicles carry cargo between intracellular compartments, to and from the cell surface, and to the extracellular environment. Many conserved families of proteins are required for properly localized vesicle fusion, including the multi-subunit tethering complexes and the SNARE complexes. These protein complexes work together to promote proper vesicle fusion in other trafficking pathways. Contrary to these other pathways, our lab previously suggested that the exocyst subunit Sec6, a component of the exocytosis-specific tethering complex, inhibited Sec9:Sso1 SNARE complex assembly due to interactions in vitro with the SNARE protein Sec9 (Sivaram et al., 2005). My goal for this project was to test the hypothesis that Sec6 inhibited SNARE complex assembly in vivo. I therefore chose to generate Sec6:Sec9 loss-of-binding mutants, and study their effect both in vitro and in vivo. I identified a patch of residues on Sec9 that, when mutated, are sufficient to disrupt the novel Sec6-SNARE interaction. Additionally, I found that the previous inhibitory role for Sec6 in SNARE assembly was due to a data mis-interpretation; my re-interpretation of the data shows that Sec6 has a mild, if any, inhibitory effect on SNARE assembly. My results suggest a potential positive role for Sec6 in SNARE complex assembly, similar to the role observed for other tether-SNARE interactions.

Стилі APA, Harvard, Vancouver, ISO та ін.

6

Dubuke, Michelle L. "The Exocyst Subunit Sec6 Interacts with Assembled Exocytic Snare Complexes: A Dissertation." eScholarship@UMMS, 2012. http://escholarship.umassmed.edu/gsbs_diss/868.

Повний текст джерела

Анотація:

In eukaryotic cells, membrane-bound vesicles carry cargo between intracellular compartments, to and from the cell surface, and to the extracellular environment. Many conserved families of proteins are required for properly localized vesicle fusion, including the multi-subunit tethering complexes and the SNARE complexes. These protein complexes work together to promote proper vesicle fusion in other trafficking pathways. Contrary to these other pathways, our lab previously suggested that the exocyst subunit Sec6, a component of the exocytosis-specific tethering complex, inhibited Sec9:Sso1 SNARE complex assembly due to interactions in vitro with the SNARE protein Sec9 (Sivaram et al., 2005). My goal for this project was to test the hypothesis that Sec6 inhibited SNARE complex assembly in vivo. I therefore chose to generate Sec6:Sec9 loss-of-binding mutants, and study their effect both in vitro and in vivo. I identified a patch of residues on Sec9 that, when mutated, are sufficient to disrupt the novel Sec6-SNARE interaction. Additionally, I found that the previous inhibitory role for Sec6 in SNARE assembly was due to a data mis-interpretation; my re-interpretation of the data shows that Sec6 has a mild, if any, inhibitory effect on SNARE assembly. My results suggest a potential positive role for Sec6 in SNARE complex assembly, similar to the role observed for other tether-SNARE interactions.

Стилі APA, Harvard, Vancouver, ISO та ін.

7

Kovrugin, Vadim M. "Cristallochimie de nouveaux composés d’oxyde du Se4+ et du Se6+." Thesis, Lille 1, 2015. http://www.theses.fr/2015LIL10072/document.

Повний текст джерела

Анотація:

Ce manuscrit est consacré à la synthèse et la caractérisation de nouveaux matériaux d'oxyde à base de sélénium dans les états d’oxydation +4 ou +6. Les types structuraux rencontrés parmi les 33 oxy sélénites minéraux montrent une diversité structurale étonnante, et couvrent le champ des dimensionnalités 0D à 3D, offrant ainsi des potentialités inexploitées en termes des propriétés physiques. Cette thèse a visé la synthèse de l’étude de nouveaux composés du Se, en utilisant des méthodes de synthèse essentiellement inspirées des conditions de croissance des minéraux. Les informations basées sur l’assemblage de briques élémentaires originales dans des architectures structurales ont été déduites et comparées aux données de la littérature sur des phases proches. Lors de ce travail, notre approche « géo-inspirée » innovante été appliquée afin d'obtenir de nouveaux polytypes complexes. Nous avons donc utilisé des procédés simulant les conditions de croissance des minéraux. En termes de description, nous avons également utilisé soit le modèle « standard » basé sur l’examen des structures cristallines en termes de coordinations de cations, soit des outils plus modernes basés sur l’assemblage de tétraèdres anions-centrés développée par l'école de la cristallographie de Saint-Pétersbourg et par le groupe de chimie du solide de l’UCCS, à Lille, et ce dans les cas où l'interprétation structurale traditionnelle ne reflète pas les principes de base de la cristallochimie. Finalement, plusieurs systèmes chimiques métal-oxyde avec du Se4+/6+ et divers métaux (Cu+/2+, Ni2+, Co2+, V4+/5+, Mn2+, Fe3+, Pb2+, Bi3+, U6+) ont été étudiés dans le cadre de ce manuscrit. La thèse contient les résultats des procédures synthétiques et de la caractérisation cristallochimique des 39 nouveaux sélénites, sélénates et sélénite-sélénates des métaux. Les principaux résultats sont donnés et font référence aux publications plus détaillées données en annexe
This work deals with the synthesis and characterization of novel oxide materials containing selenium in the oxidation states of +4 or +6. The structural types occurring in 33 known oxoselenite minerals have an amazing variety, and cover the whole field from 0D to 3D frameworks, which offer unexploited potentialities in terms physical properties. This thesis aims to synthetize and investigate new Se based compounds, essentially using methods inspired by mineralogical processes. Information based on original building units assembled into original architectures have been deduced and compared to related inorganic phases of the literature. In the present study, our innovative so-called “geo-inspired” approach is applied in order to obtain complex novel crystalline compounds. This approach assumes emulation and modelling of natural crystal growth processes. Herein, we used either a traditional descriptive procedure based on consideration of the crystal structures in terms of coordinations of cations, or the modern theory of anion-centered tetrahedra developed by the St. Petersburg school of crystallography and the UCCS group of solid-state chemistry in Lille, in cases when the traditional structural interpretation does not reflect basic principles of crystal chemistry. Thus, several metal-oxide chemical systems with Se4+/6+ and various metals (Cu+/2+, Ni2+, Co2+, V4+/5+, Mn2+, Fe3+, Pb2+, Bi3+, U6+) were studied in the context of the present work. The thesis contains results of synthetic procedures, and crystal chemical characterization of 39 new metal selenites, selenates, and selenite-selenates. The analogy of selenite groups with phosphites was also investigated. Main results are described with reference to more detailed publications given in the appendices

Стилі APA, Harvard, Vancouver, ISO та ін.

8

Caraghiozov, Robert Alexandru <1992&gt. "Chinese Special Economic Zones (SEZ) in Africa: similar development approach, different challenges." Master's Degree Thesis, Università Ca' Foscari Venezia, 2020. http://hdl.handle.net/10579/17016.

Повний текст джерела

Анотація:

In just about 30 years, China has become one of the main actors on the international board, climbing the ladder and earning a sit among global superpowers. At the same time, it transformed into a global powerhouse in OFDI (Outgoing Foreing Direct Investment), a characteristic that is usually prerogative of countries in a more advanced development stage. This thesis will focus on China's SEZs in Africa. The first chapter will try to understand what were the conditions that allowed it to become a top 3 global investor. The second chapter will explore why these investments matter in the perspective of the "going out" strategy and the sino-african relationships. Finally, the SEZs will be analyzed both in a wider perspective to understand the process that brought to the creation of such zones and in a more specific, zone by zone, overview. The last chapter will serve as an assessment of the possible socio-economic effects on the host countries, highlighting present and future challenges.

Стилі APA, Harvard, Vancouver, ISO та ін.

9

Sezi, Sabrina [Verfasser], and H. A. [Akademischer Betreuer] Wagenknecht. "Assemblierung von Ethinylnilrot und Ethinylpyren mithilfe von DNA und Pyrrolidinyl-PNA / Sabrina Sezi. Betreuer: H.-A. Wagenknecht." Karlsruhe : KIT-Bibliothek, 2013. http://d-nb.info/1049237056/34.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

10

Krüger-Özgürdal, Seza [Verfasser], Georg [Gutachter] Juckel, and Boris [Gutachter] Suchan. "Neurophysiologische, neuropsychologische und strukturelle Auffälligkeiten im Prodromalstadium der Schizophrenie / Seza Krüger-Özgürdal ; Gutachter: Georg Juckel, Boris Suchan." Bochum : Ruhr-Universität Bochum, 2017. http://d-nb.info/114022316X/34.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

11

Rozhdestvenskaya, Anna. "KALININGRAD SPECIAL ECONOMIC ZONE : EN STUDIE AV DE SKANDINAVISKA FÖRETAGENS ERFARENHETER AV SEZ-KALININGRAD." Thesis, Uppsala University, Department of Business Studies, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8809.

Повний текст джерела

Анотація:

Bakgrund: Kaliningrad är den mest västliga ryska regionen som ligger mellan två EU-länder. I takt med den höjda levnadsstandarden och på grund av det gynnsamma geografiska läget ökar svenska företagens intresse för regionen. För att öppna upp regionens ekonomi för direkta investeringar från utlandet introducerades Special Economic Zone. Syftet med SEZ-Kaliningrad var att kompensera regionen för det isolerade läget genom tull- och skattelättnader för varor som tillverkas där. SEZ-insatsen i Kaliningrad har attraherat en del direkta investeringar, men resultaten har hittils inte varit speciellt imponerande.

Syfte: Syftet med denna uppsats är att genomföra en studie av de skandinaviska företagens erfarenheter av SEZ-Kaliningrad. I samband med detta vill författaren även undersöka hur representanter från de lokala myndigheterna, som arbetar med SEZ-frågor, upplever SEZ.

Teori: Inom uppsatsens teoretiska ramverk diskuteras de viktiga anledningarna för företagens investeringsbeslut och samband mellan statlig politik, marknadens ofullständighet och direkta investeringar i utlandet. Vidare diskuteras begreppen enklav och Special Economic Zone. Avslutningsvis framställs några kriterier för en framgångsrik Special Economic Zone.

Metod: Uppsatsens undersökning genomfördes med hjälp av den kvantitativa metoden. Svaren på enkät från sex skandinaviska bolag, som är etablerade i Kaliningrad och använder sig av SEZ-villkor, analyserades. Även två representanter från lokala myndigheter, som jobbar med SEZ-frågor, deltog i undersökningen. Resultaten av undersökningen presenterades i löpande text och illustrerades med diagram.

Analys: Resultatet av den genomförda undersökningen visar att det finns skillnader mellan de lokala myndigheternas uppfattning om hur effektivt Special Economic Zone i Kaliningrad är och de skandinaviska företagens erfarenhet av den. För att öka SEZ-åtgärdernas effektivitet krävs det större intresset från de lokala myndigheternas sida i investerarnas problem. Det krävs även djupare förståelse och högre grad av samarbete mellan parterna för att förbättra den befintliga situationen.

Nyckelord: Direkta investeringar i utlandet, Special Economic Zone, Kaliningrad

Background: Kaliningrad is the western Russian city situated between two EU countries. Due to the higher living standard and the favourable geographical position the interest from the Swedish companies in the region is growing. In order to open the region’s economy to direct investments, the Special Economic Zone was introduced in the region. The purpose with the SEZ-Kaliningrad was to compensate the region for its isolation with customs and tax reductions on goods manufactured there. The SEZ in Kaliningrad has drawn a number of direct investments, but the results have not been very impressive so far.

Purpose: The purpose of this thesis is to study the Scandinavian companies’ experience of the SEZ-Kaliningrad. In connection with this the author also wants to study how the representatives from the local authorities, who work with the SEZ matters, experience the SEZ.

Theory: Within the thesis’ theoretical framework I discuss the important grounds for the companies’ investment decisions and the relation between state policy, the market’s deficiency and the direct investments abroad. Furthermore, enclave and Special Economic Zone concepts are discussed. In conclusion, a number of criteria for a successful Special Economic Zone are presented.

Method: The survey is carried out with the help of the quantitative methodology. The answers from six Scandinavian companies, which are established in Kaliningrad and implement SEZ conditions, were analyzed. Besides that, two representatives from the local authorities, who work with the SEZ matters, took part in the survey. The results of the survey were presented in the text and illustrated with diagrams.

Analysis: The results from the survey show that there are differences between the local authorities’ understanding of the efficiency of the Special Economic Zone in Kaliningrad and the Scandinavian companies’ experience of it. To make the SEZ measures more effective a greater interest is needed from the local authorities in the investment companies’ problems. There is even a need of a better understanding and a higher level of cooperation between the parties to improve the present situation.

Keywords: Foreign Direct Investments, Special Economic Zone, Kaliningrad.

Стилі APA, Harvard, Vancouver, ISO та ін.

12

Susca, Elisa <1996&gt. "SEZ: i fattori che favoriscono lo sviluppo locale e territoriale- il caso della Polonia." Master's Degree Thesis, Università Ca' Foscari Venezia, 2020. http://hdl.handle.net/10579/18096.

Повний текст джерела

Анотація:

Alla base di questo elaborato, vi è l’analisi delle Zone Economiche Speciali (ZES) pensate come “zone di vantaggio” per l’insediamento di nuove imprese con lo scopo di attrarre investimenti, favorire la crescita economica e la creazione di occupazione. Attualmente si contano, nel mondo, circa 2.700 aree in cui, oltre ad agevolazioni fiscali, sono presenti misure di sostegno finanziario, infrastrutturale e logistico, oltre alla previsione di aspetti normativi e iter procedurali differenti da quelli in vigore all’interno dei rispettivi Paesi. L’obiettivo principale dell’elaborato è di coinvolgere il panorama internazionale verso una presa di coscienza circa l’indiscutibile sviluppo a 360° sulle potenzialità di un paese, la Polonia, che fino a poco dopo la caduta del muro di Berlino si trovava in una condizione generale di grosso svantaggio rispetto alle ben più consolidate economie occidentali. Grazie agli investimenti dell’Unione Europea, ad una forte coscienza e volontà di rivalsa collettiva e ai vantaggi delle ZES in questione, il sistema Polonia sta attraversando un periodo di incrollabile crescita economica con un PIL costantemente in crescita anche nei periodi di crisi più acuti delle economie europee e contribuendo al calo sia del deficit pubblico che in termini di disoccupazione ai minimi storici. Inoltre, nel seguente documento, si è voluto prendere in considerazione due esempi concreti di ZES di successo a sfondo internazionale (Cina vs Polonia), evidenziando le maggiori differenze tra le due zone e per quale motivo la Polonia si stia confermando una delle mete più ambite dagli imprenditori/investitori per “fare impresa”. Infine, si è posto l’accento sul fenomeno del re-shoring di alcune aziende/multinazionali, le quali decidono di riportare in alcuni casi, anche solo una parte della produzione in patria con l’obiettivo, fra i tanti, di mantenere un livello qualitativo soddisfacente agli occhi di consumatori sempre più esigenti.

Стилі APA, Harvard, Vancouver, ISO та ін.

13

Schiffer, Johannes [Verfasser], Jörg [Akademischer Betreuer] Raisch, Romeo [Akademischer Betreuer] Ortega, Veit [Akademischer Betreuer] Hagenmeyer, and Tevfik [Akademischer Betreuer] Sezi. "Stability and power sharing in microgrids / Johannes Schiffer. Gutachter: Jörg Raisch ; Romeo Ortega ; Veit Hagenmeyer ; Tevfik Sezi. Betreuer: Jörg Raisch." Berlin : Technische Universität Berlin, 2015. http://d-nb.info/1075249147/34.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

14

Нагребельний, В. П. "Проблеми розвитку законодавства України про інвестиційну діяльність". Thesis, Українська академія банківської справи Національного банку України, 2010. http://essuir.sumdu.edu.ua/handle/123456789/62232.

Повний текст джерела

Анотація:

Основним суб’єктом регулювання інвестиційних відносин є держава, а звідси – очевидна об’єктивна необхідність істотного поліпшення її дієздатності через механізми законодавчого регулювання в інвестиційній сфері.
The main subject of regulation of investment relations is the state, and hence - there is an obvious objective need for a substantial improvement in its capacity through the mechanisms of legislative regulation in the investment sphere.

Стилі APA, Harvard, Vancouver, ISO та ін.

15

Gkountaras, Aris [Verfasser], Sibylle [Akademischer Betreuer] Dieckerhoff, Sibylle [Gutachter] Dieckerhoff, Friedrich [Gutachter] Fuchs, Nikos [Gutachter] Hatziargyriou, and Tevfik [Gutachter] Sezi. "Modeling techniques and control strategies for inverter dominated microgrids / Aris Gkountaras ; Gutachter: Sibylle Dieckerhoff, Friedrich Fuchs, Nikos Hatziargyriou, Tevfik Sezi ; Betreuer: Sibylle Dieckerhoff." Berlin : Universitätsverlag der TU Berlin, 2017. http://d-nb.info/1156177405/34.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

16

Ritter, Ana Carolina. "Envolvimento dos genes rpoS e dps na resistência ao hipoclorito de sódio e do gene ompR na resistência térmica de Salmonella enteritidis SE86." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2012. http://hdl.handle.net/10183/143372.

Повний текст джерела

Анотація:

Salmonella é um dos principais agentes de Doenças Transmitidas por Alimentos (DTA) em todo o mundo. No Rio Grande do Sul, uma cepa de Salmonella Enteritidis vem sendo identificada como o principal agente causador de DTA na última década, a qual foi nominada SE86. Quando comparada com outros sorovares de Salmonella, a SE86 demonstrou maior capacidade de adaptação ácida e térmica, além de maior resistência contra diferentes sanificantes. O presente estudo teve como objetivo investigar o envolvimento dos genes rpoS e dps na resistência ao hipoclorito de sódio e do gene ompR na adaptação ácida e resistência térmica da cepa S. Enteritidis SE86. Os genes avaliados foram atenuados pela técnica de Knockout e a expressão dos mesmos foi investigada pela técnica Epitope Tagging - 3 x FLAG. Os resultados demonstraram que a cepa mutante SE86, sem a presença do gene dps ( dps), demonstrou uma maior sensibilidade em relação à cepa SE86 Wild Type (WT), após a exposição ao hipoclorito de sódio a 200 ppm. As proteínas Rpos e Dps foram ativamente expressas nas mesmas condições de exposição ao hipoclorito de sódio, demonstrando assim a relação destes genes com o estresse oxidativo provocado por hipoclorito de sódio na SE86. O envolvimento do gene ompR na resistência térmica foi investigado após a adaptação ácida de SE86 WT e do mutante ompR. As células ácido adaptadas do mutante ompR foram completamente inativadas após 240 minutos de exposição a temperatura de 52º C, enquanto que as células WT resistiram até 300 minutos de exposição. A expressão da proteína OmpR foi observada por Western blotting e confirmou a relação da adaptação ácida com a maior resistência térmica da SE86.
Salmonella is one of the main agents of foodborne diseases worldwide. In Rio Grande do Sul, a clone of Salmonella Enteritidis has been identified as the main cause of foodborne diseases in the last decade, which was named SE86. The the present study aimed to investigate the involvement of rpoS and dps genes in resistance to sodium hypochlorite and ompR gene in acid adaptation and thermal resistance of the strain S. Enteritidis SE86. The genes evaluated were attenuated by knockout technique and their expression was determined by tagged (3XFLAG) strains. The results demonstrated that strain S. Enteritidis SE86 without the presence of dps gene ( dps) showed a higher sensitivity to the wild type strain SE86 (WT) when exposed to sodium hypochlorite at 200 ppm. Furthermore, RpoS and Dps proteins were actively expressed in these experimental conditions, thus demonstrating the relationship of these genes with oxidative stress in S. Enteritidis SE86 caused by sodium hypochlorite. The involvement of ompR gene in thermal resistance was observed after the acid adaptation of S. Enteritidis SE86 WT and ompR since cells adapted acid without the presence of ompR have been completely inactivated when exposed to 240 min at temperature of 52 °C, while WT cells resisted until 300 min of exposure. The expression of the OmpR protein by western blotting confirmed the necessity of the acid adaptation for that S. Enteritidis SE86 resists to high temperatures.

Стилі APA, Harvard, Vancouver, ISO та ін.

17

Ferreira, Fernanda Stoduto. "Avaliação da multiplicação e recuperação de Salmonella enteritidis SE86 em diferentes diluentes, meios de cultura e métodos de semeadura, após exposição ao dicloroisocianurato de sódio." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2011. http://hdl.handle.net/10183/28866.

Повний текст джерела

Анотація:

No Rio Grande do Sul (RS), uma cepa de Salmonella (S.) Enteritidis (SE86) foi identificada como o principal microrganismo causador de Doenças Transmitidas por Alimentos (DTA), nos últimos anos. O objetivo deste trabalho foi avaliar a multiplicação e a recuperação da S. Enteritidis SE86 em diluentes, meios de cultura e métodos de semeadura, após a exposição ao Dicloroisocianurato de sódio (NaDCC). Em um primeiro momento, o microrganismo foi ativado em caldo BHI e exposto a 200ppm de NaDCC, por cinco minutos. Em seguida, ele foi diluído em diferentes soluções, as quais foram armazenadas a 7º C e 30º C, separadamente, sendo amostradas e analisadas microbiologicamente, a cada hora, durante seis horas. Em um segundo momento, foi avaliada a recuperação do microrganismo, antes e após exposição ao NaDCC, através de semeadura em superfície e pelo método da Camada Fina de Ágar (Thin Agar Layer - TAL), em cinco diferentes meios de cultura [Agar Triptona de Soja (TSA), Agar Verde Brilhante Manitol Lisina Cristal de Violeta (MLCB), Agar Verde Brilhante (BGA), Agar Salmonella Shigella (SS) e Agar Xilose Lisina Desoxicolato (XLD)]. Na terceira fase do estudo, foram avaliadas a multiplicação e a recuperação de dois outros sorovares de Salmonella, além da S. Enteritidis SE86, utilizando o diluente, o meio de cultura e o método de semeadura que demonstraram os melhores resultados nas fases antecedentes. Os resultados demonstraram que houve multiplicação significativa (P < 0,05) da S. Enteritidis SE86 não exposta ao NaDCC, armazenada a 30º C, nos diluentes Água peptonada (P), Água peptonada + Tween 80, Lecitina e Tiossulfato de sódio (P + N), Solução salina + Tween 80, Lecitina e Tiossulfato de sódio (SaS + N) e Água peptonada + Solução salina (P + SaS). O diluente Solução salina (SaS) não propiciou multiplicação durante as seis horas de incubação, mas manteve as células viáveis, sendo, portanto, escolhido para os demais experimentos. Células expostas e não expostas ao NaDCC não foram capazes de se multiplicar em nenhum dos diluentes testados, a 7º C. Da mesma forma, após exposição ao NaDCC, nenhuma multiplicação significativa foi observada nos diluentes armazenados a 30º C. Não houve diferença significativa (P < 0,05) nas contagens de S. Enteritidis SE86 exposta ao NaDCC quando semeada em TSA, meios seletivos ou meios seletivos adicionados de sobre camada de TSA (TAL). O meio XLD foi escolhido para os demais experimentos, uma vez que permitiu praticamente a mesma multiplicação que o meio TSA. Quando foram avaliadas a multiplicação e a recuperação de S. Enteritidis SE86, S. Typhimurium e S. Bredeney, expostas e não expostas ao NaDCC, diluídas em SaS e semeadas em TSA, XLD e XLD + sobre camada de TSA (TAL), não houve diferença significativa entre as contagens de células obtidas nos meios e no TAL, sugerindo que a semeadura direta em XLD pode ser um método adequado para a quantificação de Salmonella exposta ao NaDCC, em condições laboratoriais.
In Rio Grande do Sul State (RS), a strain of Salmonella (S.) Enteritidis (SE86) was identified as the main causative microorganism of foodborne diseases, in recent years. The aim of this study was to evaluate diluents, media and plating methods for growth and recovery of this pathogen, after exposure to Sodium dichloroisocyanurate (NaDCC). At first, the microorganism was exposed to 200 mg kg-1 NaDCC by five minutes. Then it was diluted in different diluent solutions, which were stored at 7º C and 30° C, separately, being sampled and microbiologically analyzed for six hours. In a second step, the recovery of the microorganism before and after exposed by NaDCC was evaluated, through surface plating method and Thin Layer Agar (TAL) method, in five different culture media [Tryptic Soy Agar (TSA), Mannitol Lysine Crystal Violet Brilliant Green Agar (MLCB); Brilliant Green Agar (BGA), Salmonella Shigella Agar (SS) and Xylose Lysine Desoxychole Agar (XLD)]. In the third phase of this study, the growth and recovery of S. Enteritidis SE86 and two other serovars of Salmonella were evaluated, using the diluent, the culture medium and plating method that showed the best results in previous phases. The results showed significant multiplication (P < 0.05) of S. Enteritidis SE86 not exposed to NaDCC, stored at 30 °C, in diluents Peptone water (P), Peptone water + Tween 80, Lecithin and Sodium thiosulfate (P + N), Saline solution + Tween 80, Lecithin and Sodium thiosulfate (SaS + N) and Peptone water + Saline solution (P + SaS). Saline solution (SaS) did not sustain bacterial multiplication, but maintained viable cells, being chosen for the next experiments. Exposed and not exposed cells were not able to multiply in any of the diluents at 7º C during six hours of storage. After NaDCC exposure, no significant multiplication was observed in any of the diluents stored at 30º C. No significant difference (P < 0,05) in growth of S. Enteritidis SE86 exposed to NaDCC was observed on TSA, selective medium or on selective media overlayed with TSA (TAL). The XLD medium was chosen for the next experiments, since it allowed the same multiplication that TSA. When the multiplication and recovery of exposed and not exposed S. Enteritidis SE86, S. Typhimurium, and S. Bredeney were evaluated after dilution in SaS and plating on TSA, XLD, and XLD overlayed with TSA, there was no significant difference in counts obtained on media and TAL, suggesting that direct plating on XLD could be an adequate method for the quantification of Salmonella exposed to NaDCC, under laboratory conditions.

Стилі APA, Harvard, Vancouver, ISO та ін.

18

Elias, Susana de Oliveira. "Modelagem dos parâmetros cinéticos de multiplicação de Salmonella enteritidis SE86 em maionese caseira e práticas de preparo, estocagem e consumo desse alimento no Rio Grande do Sul." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2014. http://hdl.handle.net/10183/103946.

Повний текст джерела

Анотація:

Uma cepa específica de Salmonella Enteritidis (SE86) tem sido iden-tificada como o principal agente etiológico responsável pela salmonelose, no Rio Grande do Sul (RS) e o principal alimento envolvido vem sendo a maionese caseira (MC). Assim, este estudo objetivou modelar a multiplicação de SE86 na MC e estudar as práticas de preparo, estocagem e consumo de MC no RS. SE86 foi inoculada na MC e armazenada a 7, 10, 15, 20, 25, 30 e 37°C. Curvas de multiplicação foram construídas utilizando-se o DMFit de Baranyi, gerando valores de R2 maiores que 0,98 para os modelos primários. Já o modelo se-cundário foi realizado por meio da equação de Ratkowsky, obtendo valores de R2 e RMSE de 0,99 e 0,016, respectivamente. Os dados experimentais mostraram que SE86 não se multiplicou na MC a 7°C, durante 30 dias, enquanto que a 37°C alcançou-se a dose infectante (106 UFC/g) em 6 h e a população final (109 UFC/g) em 13 h. As fases lag de SE86 na MC foram menores que as si-muladas nos modelos terciários, sugerindo que SE86 adapta-se rapidamente nesse alimento. Além disso, o questionário que avaliou as práticas adotadas por consumidores e manipuladores de MC demonstrou que a maioria dos en-trevistados apresentou um nível baixo de IPShp (índice de prevenção de surto harmônico ponderado), principalmente devido a falhas relacionadas ao binô-mio tempo e temperatura. Em suma, o modelo desenvolvido pode ser utilizado para prever a multiplicação de SE86 na MC em várias temperaturas e MC pode ser preparada se forem utilizados ovos seguros e mantendo-a abaixo de 7°C.
A specific strain of Salmonella Enteritidis (SE86) has been identified as the major etiological agent responsible for salmonellosis, in Rio Grande do Sul (RS) and the main food involved has been the homemade mayonnaise (HM). This study aimed to model growth of SE86 on HM and to analyze practic-es of preparation, storage and consumption of HM in RS. SE86 was inoculated on HM and stored at 7, 10, 15, 20, 25, 30 and 37°C. Growing curves were built by fitting data to the Baranyi’s DMFit, generating R2 values greater than 0.98 for primary models. Secondary model was fitted with Ratkowsky equation, generat-ing R2 and RMSE values of 0.99 and 0.016, respectively. Experimental data showed that SE86 did not grow on HM at 7°C, for 30 days, while at 37°C infec-tive dose (106 CFU) was reached at 6 h and final population (109 CFU/g) was attained at 13 h. Experimental data demonstrated smaller lag phases than those generated by tertiary models, suggesting that SE86 is well adapted for growing on HM. In addition, the questionnaire that assessed the practices adopted by HM consumers and handlers showed that the majority of respond-ents had a low level of IPShp (outbreak prevention weighted harmonic index), mainly due to failures related to the combination of time and temperature. In conclusion, the developed model can be used to predict growth of SE86 on HM under various temperatures and HM can be prepared if safe eggs were used and HM were stored below 7°C.

Стилі APA, Harvard, Vancouver, ISO та ін.

19

Luzzu, Josto. "Dealing with the Future Experimental governance in the Golden Triangle Special Economic Zone." Thesis, The University of Sydney, 2021. https://hdl.handle.net/2123/25440.

Повний текст джерела

Анотація:

This thesis is an empirical exploration of an experimental form of governance emerging in the Golden Triangle Special Economic Zone (GTSEZ), located in northwest Laos, at the border with Thailand and Myanmar. Operations for the establishment of the zone started in 2007, following a 99-year concession agreement between the Lao government and the Kings Romans Group (KRG), a Chinese private gaming company registered in Hong Kong. KRG’s project revolves around a large casino that caters mainly for Chinese tourists, and mobilizes desires for modernity, development and prosperity in the Golden Triangle region, historically seen as lawless borderland and dependent on illicit activities such as the drug trade. The agreement extends neoliberal market logics to the governance of the region by devolving aspects of Lao sovereign power over to the foreign developer. I investigate how attempts to legitimise and regulate the encounter between state and market produce an ambivalent legal framework that foregrounds the emergence of asymmetrical power relations among the variegated range of the zone’s actors. I examine the multiple dimensions that mark the partnership between state and market in unleashing and governing arising issues that include land grabbing, exploitation of the labour force, ethnic discrimination, as well as questions of maintaining social order, political legitimisation, border politics and urbanisation. I suggest that these issues represent a testing grounds a form of experimental governance in the GTSEZ, where power relations are never absolute: rather, they are subject to continuous acts of contestation, negotiation, and compromise. The empirical study of the mechanisms that underly these processes of interaction, therefore, provides us with a broader understanding on alternative forms of sovereignty, governance and power in the Golden Triangle.

Стилі APA, Harvard, Vancouver, ISO та ін.

20

Chimbombi, Ame Rebecca. "The possibility of base erosion and profit shifting through special economic zones: A critique of the South African and Kenyan SEZ regimes based on BEPS action 5." Master's thesis, University of Cape Town, 2016. http://hdl.handle.net/11427/23787.

Повний текст джерела

Анотація:

The OECD/G20's Base Erosion and Profit Shifting (BEPS) Project has been described as the most significant international tax initiative post the 2008/2009 global economic crisis. BEPS speaks to companies engaging in aggressive tax planning strategies that exploit loopholes in tax systems to make profits 'disappear' or shift them to tax jurisdictions with little or no overall corporate tax. The BEPS Project has fifteen Actions targeting various formations, computations and permutations that could potentially give rise to BEPS. BEPS Action 5 is entitled "Countering Harmful Tax Practices More Effectively Taking into Account Transparency and Substance" and is of central importance to this minor dissertation. Special Economic Zones (SEZs) are a creature of international trade law that refers to spatially delimited areas within an economy afforded favourable administrative, regulatory and fiscal benefits when compared to the rest of the economy. The term SEZ is used as an 'umbrella' or 'label' encompassing various types of spatially delimited areas with favourable conditions. Examples of SEZs are Free Trade Zones (FTZs) and Export Processing Zones (EPZs). Although this minor-dissertation focuses mainly on tax benefits associated with SEZs, SEZs usually encompasses a wider range of benefits to the companies they host. Such other benefits could include a one-stop shop for setting up and processing work permits. This minor-dissertation examines whether South Africa and Kenya's SEZs create conducive environments for harmful tax practices in light of and as described in BEPS Action 5.

Стилі APA, Harvard, Vancouver, ISO та ін.

21

Zschau, Annette Y. "A comparative profile of the Kaliningrad (Russia) and GdanÌsk (Poland) regions as investment environments for foreign small to medium-sized enterprises, with additional reference to Russia's and Poland's SEZ regimes." Thesis, University of Oxford, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.431454.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

22

Angwafo, Tsi Evaristus. "Status of wildlife and its utilisation in Faro and Benoué National Parks North Cameroon: case study of the Derby eland (Taurotragus derbianus gigas Gray, 1947) and the African wild dog (Lycaon pictus Temminck, 1840)." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2006/11.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

23

Jurk, Steffen. "A simultaneous execution scheme for database caching." [S.l.] : [s.n.], 2005. http://se6.kobv.de:8000/btu/volltexte/2006/22.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

24

Schölzel, Mario. "Automatisierter Entwurf anwendungsspezifischer VLIW-Prozessoren." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2006/39.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

25

Sánchez, Sánchez David. "Key management for wireless ad hoc networks." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2006/16.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

26

Lupina, Grzegorz. "Praseodymium silicate high-k dielectrics on Si(001)." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2007/42.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

27

Enculescu, Mihaela. "Wellenausbreitung in neuronalen Feldern mit retardierter Wechselwirkung." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2007/43.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

28

Krawczynski, Rene. "Succession of collembola in the post mining landscape of Lower Lusatia." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2006/38.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

29

Sohal, Rakesh. "CVD growth of (001) and (111) 3C-SiC epilayers and their interface reactivity with praseodymium oxide dielectric layers." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2006/12.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

30

Jez, Uta. "Intelligenz als Determinante menschlicher Zuverlässigkeit und deren Implikation in die Entwicklung von Führungskräften." [S.l.] : [s.n.], 2005. http://se6.kobv.de:8000/btu/volltexte/2006/2.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

31

Burkhard, Carina. "TQM-Trend-Matrix Methode zur prognostischen Analyse unternehmensspezifischer Wirkungen von TQM-Massnahmen /." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2007/41.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

32

Wongdeethai, Angkarn. "The development of modern management tools and their specific requirements for the assessment of recyclability of products recycling oriented assessment tool ReOAT V.O.9 /." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2006/5.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

33

Dobelmann, Jan Kai. "Kohlenstoffelimination in bewachsenen Bodenfiltern ein empirischer Ansatz zur Vorhersage /." [S.l.] : [s.n.], 2005. http://se6.kobv.de:8000/btu/volltexte/2006/4.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

34

Schuster, Werner Klaus. "Ermittlung und Anwendung der Einwanderungskinetik von Wasser, Schmutz-Wasser und Heizöl in Porenbetone." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2006/8.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

35

Pritzkow, Olaf. "Untersuchungen zum Um- und Durchströmungsverhalten von einzelnen Agglomeratstrukturen." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2006/18.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

36

Weiss, Patrice. "Ein Modell zur Simulation der transienten Vorgänge einer Asynchronlinearmaschine Parametrierung am Beispiel eines Wirbelstromläufers /." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2006/17.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

37

Ahlinder, Stefan. "On modelling of compact tube bundle heat exchangers as porous media for recuperated gas turbine engine applications." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2006/15.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

38

Bähr, Susanne. "Experimentelle Untersuchungen zu Grundprozessen der Pelletierungsflockung." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2006/34.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

39

Vasylyev, Andriy. "Integrated RF power amplifier design in silicon based technologies." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2006/20.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

40

Nguyen, Tuan Anh. "Application of optimization methods to controller design for active suspensions." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2006/26.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

41

Krüger, Thomas. "Beitrag zum systematischen Steuerungsentwurf von Mehrmotorenantriebssystemen am Beispiel eines Laborversuchsstandes mit fahrzeugspezifischen Eigenschaften." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2006/23.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

42

Heuer, Henning. "Auf Tantal-Silizium basierende Diffusionsbarrieren zur Verkapselung funktionaler Bauelemente." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2006/1.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

43

Namango, Saul Sitati. "Development of cost effective earthen building material for housing wall construction: investigations into the properties of compressed earth blocks stabilized with sisal vegetable fibres, cassava powder and cement compositions." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2006/6.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

44

Tavakoli, Mohammad Hossein. "Numerical analysis of seeding process during Czochralski growth of oxide single crystals." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2006/14.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

45

Koch, Thomas. "Zur empirisch, analytischen Bestimmung von Verlustbeiwerten selbstorganisierender Rauheitsstrukturen in Druckrohrleitungen." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2007/45.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

46

Ewusi, Anthony. "Groundwater exploration and management using Geophysics: northern region of Ghana." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2006/37.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

47

Colas, Jean Philippe. "Strategiegenese bei Architekten und Ingenieuren." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2007/49.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

48

Röring, Nicole. "Bauhistorische Untersuchungen am Almaqah-Heiligtum von Sirwah. Vom Kultplatz zum Heiligtum." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2007/95.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

49

John, Alexander. "Zum Einfluss der Führungsqualität auf die menschliche Zuverlässigkeit in Teamstrukturen sozio-technischer Systeme." [S.l.] : [s.n.], 2007. http://se6.kobv.de:8000/btu/volltexte/2007/130.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

50

Beck, Christoph. "Untersuchung der Auswirkung von Luftummantelung und Pumpencharakteristik auf die Schneidkraft des Düsenstrahls." [S.l.] : [s.n.], 2006. http://se6.kobv.de:8000/btu/volltexte/2007/172.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Дисертації з теми "SEZ6"

Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями