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Статті в журналах з теми "Schistosomose – Diagnostic"
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HOLA-JAMRISKA, L., J. P. DALTON, J. AASKOV, and P. J. BRINDLEY. "Dipeptidyl peptidase I and III activities of adult schistosomes." Parasitology 118, no. 3 (March 1999): 275–82. http://dx.doi.org/10.1017/s0031182098003746.
Повний текст джерелаАнотація:
Soluble extracts of adult Schistosoma japonicum and S. mansoni were examined for the presence of proteolytic activities ascribable to dipeptidyl peptidases (DPPs) at a range of pH from 4 to 11 using synthetic peptidyl substrates diagnostic of DPPs I, II, III and IV. Activity capable of cleaving the DPP I-specific substrates H-Gly-Arg-NHMec and H-Gly-Phe-NHMec which exhibited a pH optimum of 5·5 was observed in extracts of schistosomes. Female schistosomes exhibited greater DPP I activity than male schistosomes, while female S. japonicum showed substantially more activity than female S. mansoni. The specific activities against H-Gly-Arg-NHMec were 21·5 and 1·9 nmoles NHMec/min/mg protein for female and male S. japonicum and 8·5 and 1·9 nmoles NHMec/min/mg for female and male S. mansoni. The biochemical properties of schistosome DPP I were similar to mammalian DPP I (=cathepsin C) in that schistosome DPP I was only slowly inhibited by the cysteine protease inhibitor trans-epoxysuccinyl-1-leucylamido (4-guanidino)- butane, partly inhibited by the blocked diazomethyl ketones Z-Phe-Ala-CHN2 and Z-Phe-Phe-CHN2, but enhanced by halide ions. At pH 8·5, activity against the DPP III-specific substrate H-Arg-Arg-NHMec was evident in schistosome extracts, and this activity appeared to be due to a zinc metallo-exopeptidase because it was inhibited by 1,10-phenathroline and by EDTA. DPP II or DPP IV activity was not detected in the schistosome extracts.
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Ruppel, Andreas, Ute Breternitz, and Reinhard Burger. "Diagnostic Mr 31 000 Schistosoma mansoni proteins: requirement of infection, but not immunization, and use of the “miniblot” technique for the production of monoclonal antibodies." Journal of Helminthology 61, no. 2 (June 1987): 95–101. http://dx.doi.org/10.1017/s0022149x00009810.
Повний текст джерелаАнотація:
ABSTRACTAntibodies directed against diagnostic Mr 31 000 polypeptide(s) of adult Schistosoma mansoni were already formed in mice during prepatency. In contrast, repeated immunization of mice with homogenates of adult schistosomes failed to elicit antibodies detectable in immunoblots in the Mr 31 000 region. Therefore, spleen cells of infected mice were used to produce hybridoma lines. The “miniblot technique” was developed in order to detect in hybridoma supernatants antibodies against schistosome Mr 31 000 components. Electrophoretically separated total S. mansoni proteins were transferred onto nitrocellulose, and the position of the Mr 31 000 components was determined with polyclonal antisera and immunoblotting. Pieces of about 3 square mm of nitrocellulose bearing the diagnostic proteins were incubated with about 100 μ1 of hybridoma supernatant in microtitre plates and subsequently probed with peroxidase-conjugated antibody to mouse IgG. This screening technique identified hybridomas secreting antibody to the relevant S. mansoni antigens. It is applicable to other defined parasit antigens, which are, however, not available in biochemically purified form. The monoclonal antibodies produced against the proteins with diagnostic potential reacted with antigens localized in the schistosome gut.
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Lakshmanan, B., K. Devada, S. Joseph, T. V. Aravindakshan, and L. Sabu. "Copro-PCR based detection of bovine schistosome infection in India." Journal of Helminthology 90, no. 1 (January 14, 2015): 102–7. http://dx.doi.org/10.1017/s0022149x1400090x.
Повний текст джерелаАнотація:
AbstractSchistosomosis and amphistomosis are the two economically important and widely prevalent snail-borne trematode infections in grazing cattle of southern India. Acute infections are symptomatically similar and difficult to detect by routine microscopy for eggs. The present study was directed towards the development of a copro-polymerase chain reaction (copro-PCR) for detection of bovine schistosome species, using custom-designed primers targeting 18S and 28S ribosomal RNA as well as mitochondrial DNA. The study demonstrated the enhanced diagnostic specificity of mitochondrial DNA markers over ribosomal RNA genes as genus-specific probes to detect schistosomes. We developed a sensitive PCR assay using primers designed from mitochondrial DNA sequences targeting the partialrrnl(16S rRNA), tCys (transfer RNA for cysteine) and partialrrnS(12S rRNA) genes ofSchistosoma spindaleto specifically detect schistosome infection from faecal samples of naturally infected bovines. The salient findings of the work also throw light on to the high similarity of the ribosomal RNA gene sequences of schistosomes with those ofGastrothylax crumeniferandFischoederius elongatus,the most prevalent pouched amphistomes of the region. Further investigation has to be directed towards unravelling the complete gene sequences of 18S and 28S ribosomal RNA as well as mitochondrial DNA sequences of amphistome isolates from India.
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Kincaid-Smith, Julien, Alan Tracey, Ronaldo de Carvalho Augusto, Ingo Bulla, Nancy Holroyd, Anne Rognon, Olivier Rey, et al. "Morphological and genomic characterisation of the Schistosoma hybrid infecting humans in Europe reveals admixture between Schistosoma haematobium and Schistosoma bovis." PLOS Neglected Tropical Diseases 15, no. 12 (December 23, 2021): e0010062. http://dx.doi.org/10.1371/journal.pntd.0010062.
Повний текст джерелаАнотація:
Schistosomes cause schistosomiasis, the world’s second most important parasitic disease after malaria in terms of public health and social-economic impacts. A peculiar feature of these dioecious parasites is their ability to produce viable and fertile hybrid offspring. Originally only present in the tropics, schistosomiasis is now also endemic in southern Europe. Based on the analysis of two genetic markers the European schistosomes had previously been identified as hybrids between the livestock- and the human-infective species Schistosoma bovis and Schistosoma haematobium, respectively. Here, using PacBio long-read sequencing technology we performed genome assembly improvement and annotation of S. bovis, one of the parental species for which no satisfactory genome assembly was available. We then describe the whole genome introgression levels of the hybrid schistosomes, their morphometric parameters (eggs and adult worms) and their compatibility with two European snail strains used as vectors (Bulinus truncatus and Planorbarius metidjensis). Schistosome-snail compatibility is a key parameter for the parasites life cycle progression, and thus the capability of the parasite to establish in a given area. Our results show that this Schistosoma hybrid is strongly introgressed genetically, composed of 77% S. haematobium and 23% S. bovis origin. This genomic admixture suggests an ancient hybridization event and subsequent backcrosses with the human-specific species, S. haematobium, before its introduction in Corsica. We also show that egg morphology (commonly used as a species diagnostic) does not allow for accurate hybrid identification while genetic tests do.
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Weerakoon, Kosala, Catherine Gordon, and Donald McManus. "DNA Diagnostics for Schistosomiasis Control." Tropical Medicine and Infectious Disease 3, no. 3 (August 1, 2018): 81. http://dx.doi.org/10.3390/tropicalmed3030081.
Повний текст джерелаАнотація:
Despite extensive efforts over the last few decades, the global disease burden of schistosomiasis still remains unacceptably high. This could partly be attributed to the lack of accurate diagnostic tools for detecting human and animal schistosome infections in endemic areas. In low transmission and low prevalence areas where schistosomiasis elimination is targeted, case detection requires a test that is highly sensitive. Diagnostic tests with low sensitivity will miss individuals with low infection intensity and these will continue to contribute to transmission, thereby interfering with the efficacy of the control measures operating. Of the many diagnostic approaches undertaken to date, the detection of schistosome DNA using DNA amplification techniques including polymerase chain reaction (PCR) provide valuable adjuncts to more conventional microscopic and serological methods, due their accuracy, high sensitivity, and the capacity to detect early pre-patent infections. Furthermore, DNA-based methods represent important screening tools, particularly in those endemic areas with ongoing control where infection prevalence and intensity have been reduced to very low levels. Here we review the role of DNA diagnostics in the path towards the control and elimination of schistosomiasis.
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Nwoko, Onyekachi Esther, John J. O. Mogaka, and Moses John Chimbari. "Challenges and Opportunities Presented by Current Techniques for Detecting Schistosome Infections in Intermediate Host Snails: A Scoping Review." International Journal of Environmental Research and Public Health 18, no. 10 (May 19, 2021): 5403. http://dx.doi.org/10.3390/ijerph18105403.
Повний текст джерелаАнотація:
Schistosomiasis, a neglected tropical disease (NTD), causes morbidity and mortality in over 250 million people globally. And 700 million people are at risk of contracting it. It is caused by a parasite of the genus Schistosoma. Freshwater snails of the family Planorbidae are of public health significance as they are intermediate hosts of these highly infective flukes. Accurate diagnostic techniques to detect schistosome infections in intermediate host snails (IHS) and environmental surveillance are needed to institute measures for the interruption of transmission and eventual elimination. We carried out a systematic review of the literature to assess advantages and limitations of different diagnostic techniques for detecting schistosome infections in snails. Literature from Scopus, Web of Science, and PubMed databases from 2008 to 2020 were searched using combinations of predefined search terms with Boolean operators. The studies revealed that conventional diagnostics are widely used, although they are labor-intensive, have low specificity and sensitivity levels, and cannot detect prepatent infections. Whereas more advanced techniques such as immunological, nucleic-acid amplification, and eDNA diagnostics have high sensitivity and specificity levels, they are costly, hence, not suitable for field applications and large-scale surveys. Our review highlights the importance of designing and developing innovative diagnostics that are high in specificity and sensitivity as well as affordable and technically feasible for use in field laboratories and for large-scale surveys.
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FLORES, VERÓNICA, GUSTAVO VIOZZI, LAURA CASALINS, ERIC SAMUEL LOKER, and SARA VANESSA BRANT. "A new schistosome (Digenea: Schistosomatidae) from the nasal tissue of South America black-necked swans, Cygnus melancoryphus (Anatidae) and the endemic pulmonate snail Chilina gibbosa." Zootaxa 4948, no. 3 (March 22, 2021): 404–18. http://dx.doi.org/10.11646/zootaxa.4948.3.5.
Повний текст джерелаАнотація:
To date, 9 species of Schistosomatidae have been found parasitizing the nasal tissues of mammal and bird hosts in the Eastern Hemisphere, 5 species in Rwanda (Africa), 2 in Australia (Oceania) and 2 in Eurasia. During a parasitological survey of black necked swans, Cygnus melancoryphus, an anatid endemic to South America, schistosome worms in the nasal tissue were found; the first in the Americas. Morphological results based on male worms and in isolated eggs. The worms have a spiny tegument, filiform body with rounded posterior end, two muscular suckers, a robust gynaecophoric channel with thickened cross bands, and around 130 testes. The eggs are elongate with an asymmetrical bulge, with a slender process at one end and a longer curved process at the other. Diagnostic morphological characteristics do not match with any schistosome genus. Part of the mitochondrial cox1 and nuclear DNA 28S partial genes were sequenced and compared to Schistosomatidae in GenBank. The genetic results confirm the distinctiveness of the specimens since they do not group with any described genus or undescribed lineage other than cercariae of “Chilina lineage 1” that emerge from the Patagonian Chilina gibbosa, a freshwater snail endemic to South America. Based on morphological and genetic characterization of these schistosomes, these specimens represent a new genus and species that parasitizes black necked swans as adults in the nasal tissue, and C. gibbosa is the first intermediate host, both hosts being endemic to South America.
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Hamouda, Ouanassa, Noureddine Boukhrouf, and Souad Bensassi. "Urinary Schistosoma haematobium schistosomiasis. A case report." Batna Journal of Medical Sciences (BJMS) 5, no. 1 (December 25, 2018): 84–86. http://dx.doi.org/10.48087/bjmscr.2018.5120.
Повний текст джерелаАнотація:
La schistosomiase urinaire est une infection intravasculaire causée par un trématode (ver plat) Schistosoma haematobium. Les vers adultes migrent généralement vers le plexus veineux de la vessie humaine et excrètent les oeufs que la personne infectée élimine dans l'urine. Nous rapportons le cas d’un patient âgé de 20 ans, avec des antécédents familiaux de bilharziose. Originaire du Mali et résidant à Batna (Algérie), il a présenté une douleur pelvienne, des brûlures mictionnelles et une hématurie terminale, avec une hyperéosinophilie de 920 / μL. L´échographie abdomino-pelvienne a mis en évidence une petite formation tissulaire bourgeonnante du plancher vésical. Une cystoscopie avec une biopsie vésicale étaient utiles pour poser le diagnostic de la schistosomiase urinaire avec la mise en évidence au sein d´un granulome inflammatoire des oeufs de Schistosoma haematobium. Le diagnostic de la maladie a été confirmé par la détection et l'identification des oeufs de Schistosoma haematobium vivant dans l'urine et dans les coupes histologiques vésicales. Le patient a reçu deux cures de 2 semaines d'intervalle de Praziquentel 40 mg / kg de poids corporel en une seule dose orale (biltricide 600 mg quatre fois). Il a continué à jeter des oeufs vivants après le premier traitement, mais aucun oeuf n'a été trouvé dans des échantillons d'urine de 24 heures après la deuxième dose de Praziquentel.
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YOU, HONG, and DONALD P. MCMANUS. "Vaccines and diagnostics for zoonotic schistosomiasis japonica." Parasitology 142, no. 2 (October 31, 2014): 271–89. http://dx.doi.org/10.1017/s0031182014001310.
Повний текст джерелаАнотація:
SUMMARYSchistosomiasis is one of the most prevalent, insidious and serious of the tropical parasitic diseases. Although the effective anthelmintic drug, praziquantel, is widely available and cheap, it does not protect against re-infection, drug-resistant schistosome may evolve and mass drug administration programmes based around praziquantel are probably unsustainable long term. Whereas protective anti-schistosome vaccines are not yet available, the zoonotic nature of Schistosoma japonicum provides a novel approach for developing a transmission-blocking veterinary vaccine in domestic animals, especially bovines, which are major reservoir hosts, being responsible for up to 90% of environmental egg contamination in China and the Philippines. However, a greater knowledge of schistosome immunology is required to understand the processes associated with anti-schistosome protective immunity and to reinforce the rationale for vaccine development against schistosomiasis japonica. Importantly as well, improved diagnostic tests, with high specificity and sensitivity, which are simple, rapid and able to diagnose light S. japonicum infections, are required to determine the extent of transmission interruption and the complete elimination of schistosomiasis following control efforts. This article discusses aspects of the host immune response in schistosomiasis, the current status of vaccine development against S. japonicum and reviews approaches for diagnosing and detecting schistosome infections in mammalian hosts.
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Wilson, R. Alan. "Schistosomiasis then and now: what has changed in the last 100 years?" Parasitology 147, no. 5 (January 22, 2020): 507–15. http://dx.doi.org/10.1017/s0031182020000049.
Повний текст джерелаАнотація:
AbstractOnly with the completion of the life cycles of Fasciola hepatica in 1883 and 30 years later those of Schistosoma japonicum (1913), Schistosoma haematobium and Schistosoma mansoni (1915) did research on schistosomiasis really get underway. One of the first papers by Cawston in 1918, describing attempts to establish the means of transmission of S. haematobium in Natal, South Africa, forms the historical perspective against which to judge where we are now. Molecular biology techniques have produced a much better definition of the complexity of the schistosome species and their snail hosts, but also revealed the extent of hybridization between human and animal schistosomes that may impact on parasite adaptability. While diagnostics have greatly improved, the ability to detect single worm pair infections routinely, still falls short of its goal. The introduction of praziquantel ~1982 has revolutionized the treatment of infected individuals and led directly to the mass drug administration programmes. In turn, the severe pathological consequences of high worm burdens have been minimized, and for S. haematobium infections the incidence of associated squamous cell carcinoma has been reduced. In comparison, the development of effective vaccines has yet to come to fruition. The elimination of schistosomiasis japonica from Japan shows what is possible, using multiple lines of approach, but the clear and present danger is that the whole edifice of schistosome control is balanced on the monotherapy of praziquantel, and the development of drug resistance could topple that.
Дисертації з теми "Schistosomose – Diagnostic"
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Lardillier-Rey, Dominique. "Intérêt diagnostique d'un antigène polysaccharidique excrété dans les urines selon la technique immunologique de détection et le moment de la miction dans un foyer pur de Schistosoma mansoni." Bordeaux 2, 1990. http://www.theses.fr/1990BOR25155.
Повний текст джерела
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Blin, Manon. "Développement d'outils de diagnostic de terrain pour la détection de la schistosomiase : une approche One Health." Electronic Thesis or Diss., Perpignan, 2023. http://www.theses.fr/2023PERP0038.
Повний текст джерелаАнотація:
Il est maintenant évident que la dégradation des environnements peut favoriser la transmission des maladies infectieuses notamment en rapprochant les humains, des vecteurs ou des animaux. Concernant les Maladies Tropicales Négligées (MTN), l’OMS tente de mobiliser les institutions et la communauté scientifique en identifiant pour chacune des MTN, les lacunes existantes dans les besoins de diagnostic clinique, les critères requis pour leur développement ainsi que les stratégies à adopter pour lutter contre la maladie. Parmi elles, la schistosomiase, seconde maladie parasitaire humaine, souffre d’un manque flagrant d’outils de diagnostic alliant sensibilité et déployabilité afin de détecter les cas de faible intensité parasitaire dans les zones endémiques ; mais également pour permettre la réalisation de diagnostic animal et environnemental visant à adopter une approche intégrative dans la lutte contre la maladie. Les objectifs de cette thèse s’inscrivent dans la stratégie One Health en proposant le développement et l’application d’outils de diagnostics de terrain chez l’Homme, chez l’animal et dans l’environnement. Les efforts continus en matière de recherche, de développement de prévention, de traitement et de sensibilisation sont essentiels pour parvenir à un monde où la schistosomiase et plus généralement les MTN ne seront plus une menace pour la santé humaineIt is now evident that environmental degradation can facilitate the transmission of infectious diseases, particularly by bringing humans into closer proximity with vectors or animals. In the case of Neglected Tropical Diseases (NTDs), the World Health Organization (WHO) is endeavoring to mobilize institutions and the scientific community by identifying, for each NTD, existing gaps in clinical diagnostic needs, the criteria required for their development, and the strategies to be adopted to combat the disease. Among them, schistosomiasis, the second most prevalent human parasitic disease, suffers from a distinct lack of diagnostic tools that combine sensitivity and deployability to detect cases with low parasitic intensity in endemic areas. Additionally, such tools are needed to facilitate animal and environmental diagnosis, enabling an integrated approach to disease control. The objectives of this thesis align with the One Health strategy, proposing the development and application of field-friendly diagnostic tools for humans, animals, and the environment. Ongoing efforts in research, development, prevention, treatment, and awareness are essential to achieve a world where Schistosomiasis and other neglected tropical diseases cease to threaten human health
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Allan, Fiona Elizabeth. "Host-parasite interactions : studies on schistosome diagnosis and transmission." Thesis, University of Leeds, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.550421.
Повний текст джерелаАнотація:
The trematode Schistosoma haematobium, which causes urinary schistosomiasis in humans, is endemic to Unguja island, Zanzibar and the freshwater snail Bulinus globosus acts as the intermediate host in this setting. Hence the distribution and infectivity of these snails are directly implicated in control of the disease within the human population. In this thesis, a variety of different experiments were undertaken to assess various aspects of the transmission process encompassing S. haematobium levels in snail populations, miracidial choice patterns and development of molecular tools for assessing prepatent snail infection levels. The most effective tool for detecting S. haematobium within the snail host was Dra 1 peR assay. This assay can detect parasite DNA within susceptible and non-susceptible species of snail, with exposure to as little as a single miracidium and at 24 hours post exposure to the parasite. Patent and prepatent prevalence were determined over the course of four years in transmission sites and rate of infection was assessed by using sentinel snails. Prepatent prevalence of S. haematobium varied between 50 and 100% throughout the year. Sentinel snails were exposed to infection within a transmission site: after ,72 hours approximately a quarter of snails had been penetrated by the parasite. S. haematobium miracidia, in choice chamber experiments, showed a preference for B. globosus and Bulinus nasutus over the sympatric species, Cleopatraferruginea and Thiara scabra. High resolution IH NMR spectroscopy was able to differentiate between Bulinus species from Zanzibar and mainland Africa and between unexposed and exposed snails. Biomarkers for S. haematobium within human urine samples were not determined due to high levels of polyparasitism within the study group. These results demonstrate that while urinary schistosomiasis remains a debilitating and important disease on Zanzibar, a greater understanding of snail parasite dynamics will be critical in designing and implementing effective and sustainable control measures.
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Dawson, Emily Mae. "Development and evaluation of a rapid diagnostic test (RDT) for detection of anti-schistosome antibodies." Thesis, University of Nottingham, 2014. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.659220.
Повний текст джерелаАнотація:
Diagnosis of schistosomiasis is still widely reliant on traditional parasitological methods, i.e. the Kato-Katz faecal smear for Schistosoma mansoni and urine filtration for S. haematobium. Since these methods are insensitive, relatively laborious and expensive to perform, much effort has been expended into developing alternative ways of diagnosing the disease. Antibody-detection is the best method for diagnosis in areas of low endemicity. It has the merit of high sensitivity and is likely to be useful for schistosomiasis control as programmes are expanded and accelerated towards meeting the WHO's 2020 goals for neglected tropical diseases (NTDs). A rapid diagnostic test (RDT) for use at the point-of-care (POC) is much more likely to be useful in low-middle income countries than the current assays that are available for antibody-detection. Work has therefore begun towards developing such a test that incorporates S. mansoni cercarial transformation fluid (SmCTF) for the detection of anti -schistosome antibodies in human blood. Here it is demonstrated that SmCTF performs equivalently to S. mansoni soluble egg antigens (SmSEA) in an enzyme-linked immunosorbent assay (ELlSA) format for the detection of anti -So mansoni, anti-So haematobium and anti-So japonicum antibodies.
Книги з теми "Schistosomose – Diagnostic"
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ICGEB/TDR Symposium (1990 Shanghai, China). Immunodiagnostic approaches in schistosomiasis: Proceedings of ICGEB/TDR Symposium, Shanghai, 1990. Chichester [England]: Wiley, 1992.
Знайти повний текст джерелаЧастини книг з теми "Schistosomose – Diagnostic"
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Agrawal, Mahesh Chandra. "Parasitological Diagnosis." In Schistosomes and Schistosomiasis in South Asia, 187–213. India: Springer India, 2012. http://dx.doi.org/10.1007/978-81-322-0539-5_7.
Повний текст джерела
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"Blood Trematodes: Schistosomes." In Diagnostic Medical Parasitology, 516–51. Washington, DC, USA: ASM Press, 2015. http://dx.doi.org/10.1128/9781555819002.ch20.
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"Blood Trematodes: Schistosomes." In Diagnostic Medical Parasitology, Fifth Edition, 445–77. American Society of Microbiology, 2007. http://dx.doi.org/10.1128/9781555816018.ch17.
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Gutierrez, Yezid. "Trematodes Of The Blood Vessels-Schistosomes." In Diagnostic Pathology of Parasitic Infections with Clinical Correlations, 545–76. Oxford University PressNew York, NY, 1999. http://dx.doi.org/10.1093/oso/9780195121438.003.0023.
Повний текст джерелаАнотація:
Abstract The schistosomes are trematodes that inhabit the blood vessels of humans and animals and produce schistosomiasis or bilharziasis, the general terms applied to the infection. Schistosomiasis is one of the most important parasitic diseases in the world because of the number of individuals affected and the morbidity and mortality produced. The disease usually occurs in persons living in poor socioeconomic conditions in tropical and subtropical areas. The classification of the schistosomes is rather complicated if all species that produce zoonotic infections, especially schistosomal dermatitis in humans, are included. In general, all the species of importance to human medicine belong to the family Schistosomatidae.
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Candido, Renata R. F., Alessandra L. Morassutti, Carlos Graeff-Teixeira, Timothy G. St. Pierre, and Malcolm K. Jones. "Exploring Structural and Physical Properties of Schistosome Eggs: Potential Pathways for Novel Diagnostics?" In Advances in Parasitology, 209–37. Elsevier, 2018. http://dx.doi.org/10.1016/bs.apar.2018.03.003.
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