Дисертації з теми "Scaffold based models"
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Loira, Nicolas. "Scaffold-based Reconstruction Method for Genome-Scale Metabolic Models." Phd thesis, Université Sciences et Technologies - Bordeaux I, 2012. http://tel.archives-ouvertes.fr/tel-00678991.
Повний текст джерелаLoira, Nicolas. "Scaffold-based reconstruction method of genome-scale metabolic models." Thesis, Bordeaux 1, 2012. http://www.theses.fr/2012BOR14484/document.
Повний текст джерелаUnderstanding living organisms has been a quest for a long time. Since the advancesof the last centuries, we have arrived to a point where massive quantities of data andinformation are constantly generated. Even though most of the work so far has focusedon generating a parts catalog of biological elements, only recently have we seena coordinated effort to discover the networks of relationships between those parts. Notonly are we trying to understand these networks, but also the way in which, from theirconnections, emerge biological functions.This work focuses on the modeling and exploitation of one of those networks:metabolism. A metabolic network is a net of interconnected biochemical reactionsthat occur inside, or in the proximity of, a living cell. A new method of discovery, orreconstruction, of metabolic networks is proposed in this work, with special emphasison eukaryote organisms.This new method is divided in two parts: a novel approach to reconstruct metabolicmodels, based on instantiation of elements of an existing scaffold model, and a novelmethod of assigning gene associations to reactions. This two-parts method allows reconstructionsthat are beyond the capacity of the state-of-the-art methods, enablingthe reconstruction of metabolic models of eukaryotes, and providing a detailed relationshipbetween its reactions and genes, knowledge that is crucial for biotechnologicalapplications.The reconstruction methods developed for the present work were complementedwith an iterative workflow of model edition, verification and improvement. This workflowwas implemented as a software package, called Pathtastic.As a case study of the method developed and implemented in the present work,we reconstructed the metabolic network of the oleaginous yeast Yarrowia lipolytica,known as food contaminant and used for bioremediation and as a cell factory. A draftversion of the model was generated using Pathtastic, and further improved by manualcuration, working closely with specialists in that species. Experimental data, obtainedfrom the literature, were used to assess the quality of the produced model.Both, the method of reconstruction in eukaryotes, and the reconstructed model ofY. lipolytica can be useful for their respective research communities, the former as astep towards better automatic reconstructions of metabolic networks, and the latteras a support for research, a tool in biotechnological applications and a gold standardfor future reconstructions
Patel, Kunal. "Stiffness Gradient Scaffolds as an In Vitro Model for Stem Cell Based Cardiac Cell Therapy." University of Akron / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=akron1386725736.
Повний текст джерелаYong, Mostyn R. "Characterisation of Polycaprolatone-Based Scaffold Plus Recombinant Human Morphogenetic Protein - 2 (RHBMP-2) in an Ovine Thoracic Spine Model." Thesis, Queensland University of Technology, 2013. https://eprints.qut.edu.au/64093/1/Mostyn_Yong_Thesis.pdf.
Повний текст джерелаWhitney, G. Adam. "Characterization of the Frictional-Shear Damage Properties of Scaffold-Free Engineered Cartilage and Reduction of Damage Susceptibility by Upregulation of Collagen Content." Case Western Reserve University School of Graduate Studies / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=case1417470427.
Повний текст джерелаMaghdouri-White, Yas. "Mammary Epithelial Cells Cultured onto Non-Woven Nanofiber Electrospun Silk-Based Biomaterials to Engineer Breast Tissue Models." VCU Scholars Compass, 2014. http://scholarscompass.vcu.edu/etd/3358.
Повний текст джерелаPiccirillo, Germano Pasquale. "Development of new in vitro models based on the use of electrospun scaffolds and their imaging by multiphoton microscopy coupled with fluorescence lifetime imaging microscopy." Doctoral thesis, Universita degli studi di Salerno, 2018. http://hdl.handle.net/10556/3021.
Повний текст джерелаIn this thesis, new possible applications of electrospun scaffolds are presented. Besides, the interaction of human dermal fibroblasts (HDFs) with the materials has been investigated using multiphoton microscopy (MPM) coupled with fluorescence lifetime imaging microscopy (FLIM), using a non-invasive, marker-free approach. In the first part of the thesis, pure poly-L-lactide (PLA) scaffolds were obtained and characterized as delivery systems for Diclofenac sodium salt (DCF) and a synthetically obtained prodrug of it for the treatment of actinic keratosis. The Diclofenac prodrug was obtained via solid phase peptide synthesis using a versatile, clean, high yielding procedure. Besides, the drug encapsulation and its release from the scaffold could be imaged using MPM. Moreover, when working with the unmodified DCF we were able to control the release profile by adding small amounts of dimethyl sulfoxide. The DCF-loaded scaffold was used as a delivery system to induce in vitro cell death in HDFs. The target cells were imaged using MPM coupled with FLIM, using a non-invasive, marker-free in vitro model to investigate drug effects. In the last part of the thesis, we produced and characterized different hybrid gelatin/PLA scaffolds. In this case, the goal was to obtain well-blended scaffolds with tunable properties, such as porosity, hydrophobicity and wettability... [edited by Author]
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Tetteh, Gifty. "Polyurethane-based scaffolds for bone tissue engineering : the role of hydroxyapatite particles, solvent combinations, electrospun fibre orientations, in vivo & in vitro characterisation, and particulate leached foams for creating 3-D bone models." Thesis, University of Sheffield, 2016. http://etheses.whiterose.ac.uk/16015/.
Повний текст джерелаRamalho, Pedro Alves Douteiro Cranfield. "Preliminary design of an Achilles tendon scaffold based on a simple model approach." Master's thesis, 2018. https://repositorio-aberto.up.pt/handle/10216/116468.
Повний текст джерелаRamalho, Pedro Alves Douteiro Cranfield. "Preliminary design of an Achilles tendon scaffold based on a simple model approach." Dissertação, 2018. https://repositorio-aberto.up.pt/handle/10216/116468.
Повний текст джерелаChen, Yi-Ching, and 陳怡靜. "The Development of Competency Model and Competency-based Training Course of Scaffolder." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/3vexpu.
Повний текст джерела文藻外語大學
國際事業暨跨文化管理研究所
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Construction industry is highly labor-intensive and technically professional. The work environment is complicated and variable, therefore, the construction site often suffers from unsafe environment, poor operation methods, and unsafe personal behaviors, which result in high occupational disasters incidence. Scaffolder is clarified high-risk work in construction industry, because scaffolder needs to carry heavy materials and climing on the heights. According to the pioneer of the American occupational safety, W.H. Heinrich, who proposed the famouse “domino theory”, claiming that the factors of accidents have causality and the unsafe personal behaviors are more likely to cause accidents and injuries than unsafe environment. Also, unsafe behavior is mostly caused by the lack of corresponding knowledge and skills. Thus, the purposes of this study are to discuss and development the competency model and competency-based training course of scaffolder in order to help raise scaffolders’ competency and decrease incidence of occupational disasters. This study uses secondary data and expert focus group meeting as data collecting methods to confirm the labor and course requirements and use ADDIE model to develop the competency model whick contains 8 elements, 35 performance indicators, 32 kinds of knowledge, 21 kinds of skills, and 9 kinds of attitude. Also, the competency-based trinaing course is developed based on the competency model.
Teixeira, Filipa Raquel Castro. "Establishment of a heterotypic 3D breast cancer model based on a vascularized porous scaffold combined with an epithelial cell-laden hydrogel." Master's thesis, 2018. https://hdl.handle.net/10216/116317.
Повний текст джерелаTeixeira, Filipa Raquel Castro. "Establishment of a heterotypic 3D breast cancer model based on a vascularized porous scaffold combined with an epithelial cell-laden hydrogel." Dissertação, 2018. https://repositorio-aberto.up.pt/handle/10216/116317.
Повний текст джерелаAli, Saniya. "Biomimetic Poly(ethylene glycol)-based Hydrogels as a 3D Tumor Model for Evaluation of Tumor Stromal Cell and Matrix Influences on Tissue Vascularization." Diss., 2015. http://hdl.handle.net/10161/11387.
Повний текст джерелаTo this day, cancer remains the leading cause of mortality worldwide1. A major contributor to cancer progression and metastasis is tumor angiogenesis. The formation of blood vessels around a tumor is facilitated by the complex interplay between cells in the tumor stroma and the surrounding microenvironment. Understanding this interplay and its dynamic interactions is crucial to identify promising targets for cancer therapy. Current methods in cancer research involve the use of two-dimensional (2D) monolayer cell culture. However, cell-cell and cell-ECM interactions that are important in vascularization and the three-dimensional (3D) tumor microenvironment cannot accurately be recapitulated in 2D. To obtain more biologically relevant information, it is essential to mimic the tumor microenvironment in a 3D culture system. To this end, we demonstrate the utility of poly(ethylene glycol) diacrylate (PEGDA) hydrogels modified for cell-mediated degradability and cell-adhesion to explore, in 3D, the effect of various tumor microenvironmental features such as cell-cell and cell-ECM interactions, and dimensionality on tumor vascularization and cancer cell phenotype.
In aim 1, PEG hydrogels were utilized to evaluate the effect of cells in the tumor stroma, specifically cancer associated fibroblasts (CAFs), on endothelial cells (ECs) and tumor vascularization. CAFs comprise a majority of the cells in the tumor stroma and secrete factors that may influence other cells in the vicinity such as ECs to promote the organization and formation of blood vessels. To investigate this theory, CAFs were isolated from tumors and co-cultured with HUVECs in PEG hydrogels. CAFs co-cultured with ECs organized into vessel-like structures as early as 7 days and were different in vessel morphology and density from co-cultures with normal lung fibroblasts. In contrast to normal lung fibroblasts (LF), CAFs and ECs organized into vessel-like networks that were structurally similar to vessels found in tumors. This work provides insight on the complex crosstalk between cells in the tumor stroma and their effect on tumor angiogenesis. Controlling this complex crosstalk can provide means for developing new therapies to treat cancer.
In aim 2, degradable PEG hydrogels were utilized to explore how extracellular matrix derived peptides modulate vessel formation and angiogenesis. Specifically, integrin-binding motifs derived from laminin such as IKVAV, a peptide derived from the α-chain of laminin and YIGSR, a peptide found in a cysteine-rich site of the laminin β chain, were examined along with RGDS. These peptides were conjugated to heterobifunctional PEG chains and covalently incorporated in hydrogels. The EC tubule formation in vitro and angiogenesis in vivo in response to the laminin-derived motifs were evaluated.
Based on these previous aims, in aim 3, PEG hydrogels were optimized to function as a 3D lung adenocarcinoma in vitro model with metastasis-prone lung tumor derived CAFs, HUVECs, and human lung adenocarcinoma derived A549 tumor cells. Similar to the complex tumor microenvironment consisting of interacting malignant and non-malignant cells, the PEG-based 3D lung adenocarcinoma model consists of both tumor and stromal cells that interact together to support vessel formation and tumor cell proliferation thereby more closely mimicking the functional properties of the tumor microenvironment. The utility of the PEG-based 3D lung adenocarcinoma model as a cancer drug screening platform is demonstrated with investigating the effects of doxorubicin, semaxanib, and cilengitide on cell apoptosis and proliferation. The results from drug screening studies using the PEG-based 3D in vitro lung adenocarcinoma model correlate with results reported from drug screening studies conducted in vivo. Thus, the PEG-based 3D in vitro lung adenocarcinoma model may serve as a better tool for identifying promising drug candidates than the conventional 2D monolayer culture method.
Dissertation
Noble, Michael-Anne. "Characterization of critical thinking indicators in problem-based learning online discussions of blended and distance undergraduate environmental science students using the community of inquiry model." Thesis, 2017. https://dspace.library.uvic.ca//handle/1828/8516.
Повний текст джерелаGraduate