Дисертації з теми "Sanguinins"
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Mahoney, Brian. "Examination of platelet adhesion by Streptococcus sanguinis." VCU Scholars Compass, 2009. http://scholarscompass.vcu.edu/etd/1978.
Повний текст джерелаAtia, Sawsan. "SYSTEMATIC ANALYSIS OF ABC TRANSPORTERS IN STREPTOCOCCUS SANGUINIS." VCU Scholars Compass, 2013. http://scholarscompass.vcu.edu/etd/3054.
Повний текст джерелаCallahan, Jill. "Functional Characterization of the Streptococcus sanguinis com Regulon." VCU Scholars Compass, 2011. http://scholarscompass.vcu.edu/etd/252.
Повний текст джерелаPatel, Jenishkumar. "ENVIRONMENTAL RESPONSES OF TWO-COMPONENT SYSTEMS IN STREPTOCOCCUS SANGUINIS." VCU Scholars Compass, 2010. http://scholarscompass.vcu.edu/etd/2270.
Повний текст джерелаTurner, Lauren. "Identification of Virulence Determinants for Streptococcus sanguinis Infective Endocarditis." VCU Scholars Compass, 2008. http://scholarscompass.vcu.edu/etd/1560.
Повний текст джерелаTurner, Lauren Senty. "Identification of virulence determinants for streptococcus sanguinis infective endocarditis /." Online version not available until 8/4/2013, 2008. http://hdl.handle.net/10156/2243.
Повний текст джерелаAl, Moussawi Ali. "Reconstruction 3D de vaisseaux sanguins." Thesis, Toulon, 2014. http://www.theses.fr/2014TOUL0014/document.
Повний текст джерелаThis work concerns the 3D reconstruction of blood vessels from a limited number of 2D transversal cuts obtained from scanners. If data are missing, a coherentreconstruction with a vessel network is obtained. This approach allows to limit human interventions in processing images of 2D transversal cuts. Knowing that the images used are obtained by scanner, the difficulty is to connect the blood vessels between some widely spaced cuts in order to produce the graph corresponding to the network of vessels. We identify the vessels on each trnasversal cut as a mass to be transported, we construct a graph solution of a branched transport problem. At this stage, we are able to reconstruct the 3D geometry by using the 2D Level Set Functions given by the transversal cuts and the graph information. The 3D geometry of blood vessels is represented by the data of the Level Set function defined at any point of the space whose 0-level corresponds to the vessel walls. The resulting geometry is usually integrated in a fluid mechanic code solving the incompressible Navier-Stokes equations on a Cartesian grid strictly included in a reconstructed geometry. The inadequacy of the mesh with the interface of the geometry is overcomed thanks to a modified boundary condition leading to an accurate computation of the constraints to the walls
Xikota, João Carlos. "Contribuição ao estudo do comportamento da arteria cerebelar caudal no cão (CANIS FAMILIARIS - Linnaeus, 1758)." reponame:Repositório Institucional da UFSC, 1996. http://repositorio.ufsc.br/xmlui/handle/123456789/76540.
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O estudo da origem, trajeto e ramificação da artéria cerebelar caudal, em 40 cães, injetados com solução de Schlesinger. Resultados: em 58,75% das observações, encontramos a artéria cerebelar caudal única e em 41,25% dupla. Em 5,00% dos lados observamos a presença de uma artéria cerebelar caudal acessória. A artéria cerebelar caudal originou-se da artéria basilar em 70,80% dos casos, da artéria vertebral em 26,55% ou de um tronco vértebro-basilar em 2,65%. Correlacionando origem e número da artéria cerebelar caudal verificamos que, quando única, esta teve origem na artéria basilar em 37,50% dos lados, na vertebral em 17,50%, e no tronco vértebro-basilar em 3,75%. Quando dupla, originou-se na artéria basilar em 21,25% e em 20,00% um ramo teve origem na artéria basilar e o outro na artéria vertebral.
Jittapalapong, Sathaporn. "Immune resistance to Rhipicephalus Sanguineus in dogs /." The Ohio State University, 2000. http://rave.ohiolink.edu/etdc/view?acc_num=osu1488191124572314.
Повний текст джерелаRunnels, Cora. "Phylogeography and Species Status of Ramphogordius sanguineus." VCU Scholars Compass, 2013. http://scholarscompass.vcu.edu/etd/3165.
Повний текст джерелаRodriguez, Alejandro. "Physiological and Molecular Characterization of Genetic Competence in Streptococcus sanguinis." VCU Scholars Compass, 2008. http://scholarscompass.vcu.edu/etd/1570.
Повний текст джерелаAynapudi, Jessica. "Involvement of Signal Peptidase I in Streptococcus sanguinis Biofilm Formation." VCU Scholars Compass, 2016. http://scholarscompass.vcu.edu/etd/4451.
Повний текст джерелаSollier, Elodie. "Microsystèmes pour la préparation d'échantillons sanguins." Phd thesis, Université Joseph Fourier (Grenoble), 2009. http://tel.archives-ouvertes.fr/tel-00434954.
Повний текст джерелаChabannes, Vincent. "Vers la simulation des écoulements sanguins." Phd thesis, Université de Grenoble, 2013. http://tel.archives-ouvertes.fr/tel-00923731.
Повний текст джерелаBoileau, Adeline. "Biomarqueurs transcriptomiques sanguins des maladies cardiovasculaires." Thesis, Université de Lorraine, 2018. http://www.theses.fr/2018LORR0186/document.
Повний текст джерелаCardiovascular disease (CVD) is the main cause of mortality in the World and in Europe. Diagnosis and prediction of outcome of CVD currently rely on the use of protein biomarkers, but should be improved to optimize patient healthcare. Blood transcriptome contains all RNA molecules present in blood cells and in the acellular compartment. Among them, messenger RNA (mRNA) code for proteins whereas small non coding RNA, microRNA (miRNA), have a regulatory function by repressing the expression of their target genes. We hypothesized that blood transcriptome, mRNA and miRNA in particular, had a potential as biomarker, diagnostic or prognostic, in CVD. In a first study, we showed that endogenous heparin could lead to an inhibition of reverse transcription and quantitative PCR reaction used to measure miRNAs expressed in the blood, and that this parameter should be considered for studies on blood transcriptome. Secondly, we showed that 3 transcripts (coding for genes LMNB1, LTBP4, TGFBR1) expressed in whole blood, were independent predictors of cardiac function alteration at 4 months post-MI. Furthermore, the inclusion of these 3 transcripts in a prediction model containing clinical variables had an incremental predictive value. In a third study, we showed that circulating levels of miR-574-5p were able to discriminate patients with TAA from healthy controls. Furthermore, miR-574-5p was encapsulated in extracellular vesicles in the blood, suggesting a paracrine role. In the fourth and fifth studies, we showed that circulating levels of miR-122-5p were independent predictors of neurological outcome and survival at middle term post-CA, and were able to increase the prediction value of existing models. We also identified miR-574-5p as an independent predictor of neurological outcome post-CA, specifically in women. To conclude, this work allowed the discovery or the confirmation of the potential biomarker value of transcripts and miRNAs in different CVD. However, their biomarker value should be validated in other large scale studies and with other methods of measurement before foreseeing their clinical utilization
Dupire, Jules. "Dynamique de cellules sanguines dans des microécoulements." Thesis, Aix-Marseille, 2012. http://www.theses.fr/2012AIXM4090/document.
Повний текст джерелаThis thesis deals with dynamics of blood cells in microflow. This title regroups two aspects of my work. The first one studies the movement of red blood cells (RBC) under flow. Continuing the work done by M. Abkarian, M. Faivre and A. Viallat, we looked at RBCs in an oscillating shear flow and showed the presence of chaos in the motion (Dupire J. et al, PRL 104,168101 (2010) ). Then we continued the study of RBC under constant flow to understand the regime of motion that were still to elucidate (PNAS, accepted for publication). These works use a ellipsoidal fixed shape model (based on Keller and Skalak's) to which we add an elastic membrane term. To take into account the shape memory, we calculated again the equations of motion considering a new stress-free shape of the elastic cytoskeleton. It allows us to fit the model on the experimental data using viscosity and elasticity coefficient compatible with the litterature. The second part deals with the motion of white blood cell (WBC) in a microfluidic channel network. The device has a regular geometry and has biomimetic shape characteristics matching the human lung mean values. We aim to study how the cell's rheology is related to their motion through the device. We show how the entry of the cell, and thus their first deformation, can be used to obtain information about a single cell rheology (viscosity, elasticity, tension). The motion is then decomposed in 2 phases : a transient regime right after the entrance and a final stationary regime. We study these regimes in terms of cellular deformation and wall friction
Piranda, Eliane Mattos. "Estudos biol?gicos de Rhipicephalus sanguineus e intera??o Rickettsia rickettsii, R. sanguineus e c?es em condi??es laboratoriais." Universidade Federal Rural do Rio de Janeiro, 2008. https://tede.ufrrj.br/jspui/handle/tede/827.
Повний текст джерелаFunda??o de Amparo a Pesquisa do Estado de S?o Paulo
The bacterium Rickettsia rickettsii is the etiological agent of an acute, severe human disease called Rocky Mountain Spotted Fever in the United States or Brazilian Spotted Fever in Brazil. The infection occurs through the tick bite. Reports of clinical illness on dogs due to this agent have been restricted to the United States. The brown dog tick or kennel tick, Rhipicephalus sanguineus (Latreille, 1806) is the most widespread tick species throughout the tropics and subtropics. Biological studies on ticks are very important to the tick-borne pathogens transmission knowledge. For this purpose, the present study evaluated experimental infection of dogs with a Itaia?u (Mogi das Cruzes/ S?o Paulo, Brasil) strain of R. rickettsii and some biological aspects of Rhipicephalus sanguineus. Initially, dogs were infected with R. rickettsii its susceptibility and the role of R. sanguineus as a vector was verified. In the second part the viability of adults ticks (R. sanguineus) on three different temperatures were tested. Ticks were maintained under the controlled conditions of 18 ? 1◦C, 27 ? 1◦C e 32 ? 1◦C e 80 ? 5% (temperature ? humidity) for different times without feed. The dogs were susceptible to R. rickettsii infection. R. sanguineus was able to acquire the pathogen and to transmit R. rickettsii to guinea pigs. Based on the second part s results, R. sanguineus viability is affected by both, temperature and unfed time.
A bact?ria Rickettsia rickettsii ? o agente etiol?gico de uma doen?a aguda e severa em humanos denominada Rocky Mountain Spotted Fever nos Estados Unidos e Febre Maculosa Brasileira no Brasil. A infec??o se d? pela picada de carrapatos. Relatos da doen?a cl?nica nos c?es s?o restritos aos EUA. O carrapato marrom do c?o ou carrapato dos canis, Rhipicephalus sanguineus (Latreille, 1806), ? a esp?cie de carrapato mais freq?ente nos tr?picos e subtr?picos. Estudos da biologia de carrapatos s?o de grande import?ncia para o entendimento da transmiss?o de biogentes. O objetivo do presente trabalho ? avaliar experimentalmente a infec??o nos c?es com R. rickettsii cepa Itaia?u (Mogi das Cruzes/ S?o Paulo, Brasil) e aspectos da biologia de Rhipicephalus sanguineus. Na primeira etapa, c?es foram infectados com R. rickettsii e avaliou-se a susceptibilidade dos animais a infec??o e o comportamento de R. sanguineus como vetor. No segundo experimento, adultos de R. sanguineus tiveram a viabilidade de infestar novos hospedeiros analisada. Os carrapatos foram mantidos a18 ? 1◦C, 27 ? 1◦C e 32 ? 1◦C e 80 ? 5% UR por tr?s per?odos de jejum. Observou-se que os c?es s?o suscept?veis a infec??o por R. rickettsii cepa Itaia?u. Foi verificado que R. sanguineus se infectam com R. rickettsii e s?o capazes de transmitir o agente a cobaias. Baseado nos resultados do segundo experimento, concluem-se que R. sanguineus tem a viabilidade influenciada pela temperatura e tempo de jejum.
Baker, Shannon. "Examination of Strain-Dependent Differences in S. sanguinis Virulence and Growth." VCU Scholars Compass, 2019. https://scholarscompass.vcu.edu/etd/5707.
Повний текст джерелаGarcia, Telma Alves. "Purificação e caracterização das lacases de Pycnoporus sanguineus." reponame:Repositório Institucional da UnB, 2006. http://repositorio.unb.br/handle/10482/2253.
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O fungo Pycnoporus sanguineus foi eficiente na descoloração de alguns corantes empregados na indústria farmacêutica. Os resultados obtidos indicam que o P. sanguineus e principalmente as lacases produzidas por este fungo apresentam grande potencial para aplicações biotecnológicas. Para a produção de lacase (EC 1.10.3.2, p-difenol:dioxigênio oxidoredutase) o fungo foi cultivado em meio contendo 2,5- xilidina e cobre como indutores. Duas isoformas da enzima (Lac 1 e Lac 2) foram identificadas e separadas através de cromatografia de interação hidrofóbica. As enzimas Lac 1 e Lac 2 apresentaram uma massa molecular de 79,7 kDa e 68,1 kDa, respectivamente. As duas isoformas apresentaram características bioquímicas diferentes. A enzima Lac 1, contendo menor atividade especifica, foi parcialmente purificada. Usando seringaldazina como substrato a enzima apresentou um pH ótimo de 4,8, temperatura ótima de 25-30 °C e Km de 10,3 μmol.L-1. A enzima mostrou baixa estabilidade à temperatura de 50 °C, mantendo apenas 10% da atividade após 5 horas de incubação. A enzima Lac 2, contendo maior atividade especifica, foi purificada com um rendimento final de 13,9 %. Usando seringaldazina como substrato a enzima apresentou um pH ótimo de 4,2, temperatura ótima de 50 °C e Km de 8,3 μmol.L-1. A enzima mostrou alta estabilidade a temperatura de 50 °C, mantendo 100 % da atividade após 5 horas de incubação. Ambas as enzimas foram inibidas por azida sódica e fluoreto de sódio. _________________________________________________________________________________ ABSTRACT
Fungus Pycnoporus sanguineus was efficient in the discoloration of some dyes used in the pharmaceutical industry. Ours results indicated that the P. sanguineus and mainly laccases produced by this fungus presents great potential for biotechnological applications. For the production of laccase (EC 1.10.3.2, p-diphenol:dioxigen oxidoreductase) this fungus was cultivated in medium containing 2,5- xylidine and copper as inducer. Two isoforms of the enzyme (Lac 1 and Lac 2) had been identified and separated through chromatography on hydrophobic interaction. The enzymes Lac 1 and Lac 2 had presented a molecular mass of 68.1 kDa and 79.7 kDa, respectively. The two isoforms presented different biochemical characteristics. The enzyme Lac 1, with lower specific activity, was partially purified. Using syringaldazine as substrate the enzyme showed pH optima of 4.8, temperature optima of 25-30 °C and Km of 10.3 μmol.L-1. The enzyme showed low stability at temperature of 50 °C, keeping only 10% of the activity, after 5 hours of incubation. The enzyme Lac 2, with higher specific activity, was purified with a final yield of 13.9 %. Using syringaldazine as substrate the enzyme presented one pH optima of 4.2, temperature optima of 50 °C and Km of 8.3 μmol.L-1. The enzyme showed high stability at temperature of 50 °C, keeping 100% of the activity even after 5 hours of incubation. Both enzymes were inhibited by sodium azide and sodium fluoride.
Tazi, Myriem. "Modélisations d'écoulements sanguins au passage de bifurcations." Toulouse, INPT, 1991. http://www.theses.fr/1991INPT056H.
Повний текст джерелаCRESPEAU, HERVE. "Idiotypie des groupes sanguins du systeme ABO." Paris 6, 1992. http://www.theses.fr/1992PA066101.
Повний текст джерелаKurbatova, Polina. "Modélisation hybride de l'érythropoïèse et des maladies sanguines." Phd thesis, Université Claude Bernard - Lyon I, 2011. http://tel.archives-ouvertes.fr/tel-00752835.
Повний текст джерелаPeyre, Thi Kim Anh. "Rôle inflammatoire des plaquettes sanguines : application en transfusion." Phd thesis, Université Jean Monnet - Saint-Etienne, 2013. http://tel.archives-ouvertes.fr/tel-01058759.
Повний текст джерелаDemin, Ivan. "Modélisations mathématiques de l'hématopoïèse et des maladies sanguines." Phd thesis, Université Claude Bernard - Lyon I, 2009. http://tel.archives-ouvertes.fr/tel-00653348.
Повний текст джерелаChabert, Adrien. "Rôle inflammatoire des plaquettes sanguines lors du sepsis." Thesis, Lyon, 2017. http://www.theses.fr/2017LYSES024/document.
Повний текст джерелаDuring some years, platelets are recognized as an key element of immunity. In fact, platelets play several roles, as signal detection, immunomodulator release and interaction with other immunity cells. The clinical significance of a correlation between platelet rate and sepsis mortality reveals the importance of this cell in the pathophysiology of sepsis. Thus, the purpose of this doctoral work was to highlight the inflammatory role of platelets in the interactions between the virulence factors of Staphylococcus aureus (S. aureus) that are different exotoxins or strains of S. aureus from bacteremia of septic patients. An experimental model of murine sepsis allowed us to understand the inflammatory component played by platelets as well as their implications in pulmonary dysfunction resulting from sepsis. Finally, we evaluated the modulation of antiplatelet molecules, particularly acetylsalicylic acid, on the numerous pathogenic roles of platelets during this pathology
Demin, Ivan. "Modélisations mathématiques de l’hématopoïèse et des maladies sanguines." Thesis, Lyon 1, 2009. http://www.theses.fr/2009LYO10333/document.
Повний текст джерелаThis PhD thesis is devoted to mathematical modelling of haematopoiesis and blood diseases. We investigate several models, which deal with different and complementary aspects of haematopoiesis.The first part of the thesis concerns a multi-scale model of erythropoiesis where intracellular regulatory networks, which determine cell choice between self-renewal, differentiation and apoptosis, are coupled with dynamics of cell populations. Using experimental data on anemia in mice, we evaluate the roles of different feedback mechanisms in response to stress situations. At the next stage of modelling, spatial cell distribution in the bone marrow is taken into account, the question which has not been studied before. We describe normal haematopoiesis with a system of reaction-diffusion-convection equations and prove existence of a stationary cell distribution. We then introduce malignant cells into the model. For some parameter values the disease free solution becomes unstable and another one, which corresponds to leukaemia, appears. This leads to the formation of tumour which spreads in the bone marrow as a travelling wave. The speed of its propagation is studied analytically and numerically. Bone marrow cells exchange different signals that regulate cell behaviour. We study, next, an integro-differential equation which describes cell communication and prove the existence of travelling wave solutions using topological degree and the Leray-Schauder method. Individual based approach is used to study distribution of different cell types in the bone marrow. Finally, we investigate a Physiologically Based Pharmacokinetics-Pharmacodynamics model of leukaemia treatment with AraC drug. AraC acts as chemotherapy, inducing apoptosis of all proliferating cells, normal and malignant. Pharmacokinetics provides the evolution of intracellular AraC. This, in turn, determines cell population dynamics and, consequently, efficacy of treatment with different protocols
Kurbatova, Polina. "Modélisation hybride de l’érythropoïèse et des maladies sanguines." Thesis, Lyon 1, 2011. http://www.theses.fr/2011LYO10258/document.
Повний текст джерелаThis dissertation is devoted to the development of new methods of mathematical modeling in biology and medicine, off-lattice discrete-continuous hybrid models, and their applications to modelling of hematopoiesis and blood disorders, such as leukemia and anemia. In this approach, biological cells are considered as discrete objects while intracellular and extracellular networks are described with continuous models, ordinary or partial differential equations. Cells interact mechanically and biochemically between each other and with the surrounding medium. They can divide, die by apoptosis or differentiate. Their fate is determined by intracellular regulation and influenced by local control from the surrounding cells or by global regulation from other organs. In the first part of the thesis, hybrid models with off-lattice cell dynamics are introduced. Model examples specific for biological processes and describing competition between cell proliferation and apoptosis, proliferation and differentiation and between cell cycling and quiescent state are investigated. Biological pattern formation with hybrid models is discussed. Application to bacteria filament is illustrated. In the next chapter, hybrid model are applied in order to model erythropoiesis, red blood cell production in the bone marrow. The model includes immature blood cells, erythroid progenitors, which can self-renew, differentiate or die by apoptosis, more mature cells, reticulocytes, which influence erythroid progenitors by means of growth factor Fas-ligand, and macrophages, which are present in erythroblastic islands in vivo. Intracellular and extracellular regulation by proteins and growth factors are specified and the feedback by the hormones erythropoietin and glucocorticoids is taken into account. The role of macrophages to stabilize erythroblastic islands is shown. Comparison of modelling with experiments on anemia in mice is carried out. The following chapter is devoted to leukemia modelling and treatment. Erythroleukemia, a subtype of Acute Myeloblastic Leukemia (AML), develops due to insufficient differentiation of erythroid progenitors and their excessive slef-renewal. A Physiologically Based Pharmacokinetics-Pharmacodynamics (PBPKPD) model of leukemia treatment with AraC drug and chronotherapeutic treatments of leukemia are examined. Comparison with clinical data on blast count in blood is carried out. The last chapter deals with the passage from a hybrid model to a continuous model in the 1D case. A convergence theorem is proved. Numerical simulations confirm a good agreement between these approaches
Eymard, Nathalie. "Modélisation hybride de l’hématopoïèse et de maladies sanguines." Thesis, Lyon 1, 2014. http://www.theses.fr/2014LYO10340/document.
Повний текст джерелаThe thesis is devoted to mathematical modeling of hematopoiesis and blood diseases. It is based on the development of hybrid discrete continuous models and to their applications to investigate production of blood cell (hematopoiesis) and blood diseases such as lymphoma and myeloma. The first part of the thesis concerns production of blood cells in the bone marrow. We will mainly study production of red blood cells, erythropoiesis. In mammals erythropoiesis occurs in special structures, erythroblastic islands. Their functioning is determined by complex intracellular and extracellular regulations which include various cell types, hormones and growth factors. The results of modeling are compared with biological and medical data for humans and mice. The purpose of the second part of the thesis is to model some blood diseases, T cell Lymphoblastic lymphoma (T-LBL) and multiple myeloma (MM) and their treatment. TLBL develops in the thymus and it affects the immune system. In MM malignant cells invade the bone marrow and destroy erythroblastic islands preventing normal functioning of erythropoiesis. We developed multi-scale models of these diseases in order to take into account intracellular molecular regulation, cellular level and extracellular regulation. The response to treatment depends on the individual characteristics of the patients. Various scenarios are considered including successful treatment, relapse and development of the resistance to treatment. The last part of the thesis is devoted to a reaction-diffusion model which can be used to describe Darwinian evolution of cancer cells. Existence of pulse solutions, which can describe localized cell populations and their evolution, is proved
Nguyen, Thi Kim Anh. "Rôle inflammatoire des plaquettes sanguines : application en transfusion." Thesis, Saint-Etienne, 2013. http://www.theses.fr/2013STET014T/document.
Повний текст джерелаBlood platelets are non-nucleated cells and play a major role in primary hemostasis and a key role in inflammation, innate and adaptive immunity. They secrete a large variety of soluble factors including cytokines/chemokines (CK/CH) and immunomodulator factors. The emergence of their inflammatory role has raised numerous questions based on the ability of platelets to respond to complex stimuli. Our investigations to answer these questions were realized in the context of platelet component transfusion. In our study, we demonstrated the correlation between the platelet secretion of soluble factors with their membrane receptors and the signaling pathways involved. Platelets express many immune receptors on their surface, including "Pattern recognition receptors" (PRRs) and receptor for CK/CH. We discovered and characterized the function of a new platelet receptor, the Siglec-7. This receptor is located in the granules a and its expression is correlated to the platelet activation level. The Siglec -7 has a high avidity with the molecules composed of α2,8-disialyl (NeuAcα2,8NeuAcα2,3Gal) and of α2,6-sialyl (Gal-b1,3[NeuAcα2,6]HexNAc) (ganglioside GD2 , GD3 and GT1b). Stimulation of this platelet receptor may induce platelet apoptosis by the intrinsic and extramitochondrial pathway. This process requires the engagement of GPIIbIIIa and P2Y1 receptor and the PI3K pathway. We also demonstrated a multifactorial inflammatory component in adverse effects issuing from platelets transfusion, and identified many soluble factors which have a high predictive value of Acute Transfusion Reactions (ATR) occurrence, such as sCD40L and IL- 13. We confirmed that the concentration of these factors increases during storage time of platelet component (PC), being partly responsible for the high rate of ATR by old PC. Finally, in addition to the PC conservation, the process of PC preparation may also have impacts on the inflammatory properties of platelets. These studies showed that the platelet inflammatory response is regulated by the stimulus, explaining the sentinel role of human blood platelets. Therefore, my work contributes to the re-exploration of inflammatory function of these cells and studies their role as an immune cell with an inflammatory component
Damien, Pauline. "Plaquettes sanguines et entretien de l’inflammation post-infectieuse." Thesis, Saint-Etienne, 2013. http://www.theses.fr/2013STET018T/document.
Повний текст джерелаBlood platelets are anucleate cells which play an key role in haemostasis. In addition to this function, they participate in a number of other functions related to the inflammatory response including danger detection, cytokine release, and leukocyte transmigration. In the first part of the study, we highlight the ability of platelets to undergo an inflammatory activation response to a pathogen. Indeed during HIV infection, platelets are hyperresponsive and release immunomodulatory factors that can be involved in the inflammatory state seen in the patients. In a parallel way, platelets are also sensitive to bacteria, involving TLRs 2 and 4, exotoxins, as well as whole live bacteria. The inflammatory profile induced is sufficient, and quite diversified to participate in sepsis physiopathology. Platelet inflammatory functions also apply to their ability to crosstalk with neutrophils. Thus in the second part of our study, we focus on this interconnection, which does not appear to be stopping at the endothelial barrier, and can be seen during extravasation where neutrophils carry surface bound platelets in order to maintain inflammation directly onsite (alveolar inflammation model here).The diversity of platelet inflammatory activities highlighted in our work leads to several possibilities for the development of an antiplatelet therapeutic target which could modulate an exacerbated inflammatory response
Evans, Karra. "SYSTEMATIC STUDY OF GENE FUNCTIONS FOR MORPHOLOGICAL CHAIN FORMATION IN STREPTOCOCCUS SANGUINIS." VCU Scholars Compass, 2011. http://scholarscompass.vcu.edu/etd/2514.
Повний текст джерелаDvergsten, Erik C. "A Weighted Gene Co-expression Network Analysis for Streptococcus sanguinis Microarray Experiments." VCU Scholars Compass, 2016. http://scholarscompass.vcu.edu/etd/4430.
Повний текст джерелаMcGregor, John. "Étude structurale et fonctionnelle des glycoprotéines membranaires plaquettaires humaines : applications en pathologie." Lyon 1, 1987. http://www.theses.fr/1987LYO1T066.
Повний текст джерелаPellegrino, Junior Jordão 1953. "Biologia molecular de grupos sanguineos aplicada a medicina transfusional." [s.n.], 2001. http://repositorio.unicamp.br/jspui/handle/REPOSIP/308637.
Повний текст джерелаTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas
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Resumo: Com a finalidade de avaliarmos se as técnicas moleculares, atualmente utilizadas na genotipagem de grupos sanguíneos, poderiam ser utilizadas com segurança em uma população com antecedentes étnicos diversos, foram estudadas 250 amostras de DNA de doadores voluntários de sangue, atendidos no Centro de Hematologia e Hemoterapia da Unicamp, previamente fenotipadas para os sistemas Rh, Kell, Duffy e Kidd. Nossos resultados demonstraram concordância entre o fenótipo e o genótipo, indicando que os primers utilizados e que foram desenhados de acordo com as sequências genômicas obtidas de indivíduos com ancestrais caucasianos, podem ser empregados na determinação dos genótipos de grupos sanguíneos em populações com antecedentes étnicos diversos. No sistema Duffy, 66% das amostras genotipadas como FY B foram fenotipadas como Fy(b-), devido à mutação no promotor eritróide GATA, o que demonstra que estes indivíduos são de fato FY B e, expressam a proteína Fyb em outros tecidos. Com o objetivo ainda de correlacionar os resultados dos fenótipos e genótipos dos sistemas Rh, Kell, Duffy e Kidd, em pacientes politransfundidos, e pelo fato de acreditarmos que a genotipagem molecular pode ser uma alternativa importante na determinação do perfil antigênico de pacientes portadores de anemias, em esquema de transfusão de sangue fenotipado, selecionamos dez pacientes portadores de 'beta' talassemia homozigótica e quarenta pacientes portadores de anemia falciforme. A fenotipagem eritrocitária foi realizada por testes de hemaglutinação em gel, enquanto que a genotipagem foi realizada pelas técnicas de AS-PCR e PCR-RFLP. Discrepâncias entre o fenótipo e o genótipo foram encontradas em nove dos dez pacientes portadores de 'beta' talassemia (90%) e, em seis dos quarenta pacientes portadores de anemia falciforme (15%). Para uma maior segurança de nossos resultados, em todos os pacientes que apresentaram resultados discrepantes entre a fenotipagem e a genotipagem, novas análises foram realizadas empregando-se DNA extraído de células do epitélio bucal e, todas confirmaram os resultados previamente obtidos em DNA extraído de leucócitos de sangue periférico. Nestes casos, tivemos a oportunidade de realizar nova determinação do fenótipo nas amostras das unidades de sangue transfundidas e pudemos verificar que os fenótipos pertenciam aos doadores e não aos receptores. Nossos resultados sugerem que a genotipagem de grupos sanguíneos contribui, substancialmente,na qualidade da transfusão de sangue fenotipado, sobretudo em pacientes que necessitam de transfusões de repetição, como por exemplo, pacientes portadores de anemia falciforme ou thalassemia. Nos casos onde a discrepância de resultados foi identificada, a nova conduta hemoterápica possibilitou um aumento na sobrevida das hemácias transfundidas e um aumento nos níveis de hemoglobina dos pacientes. Em conclusão, a genotipagem de grupos sanguíneos deve ser recomendada em pacientes dependentes de transfusão pois permite a seleção correta do sangue a ser transfundido. Auxilia portanto, na prevenção da aloimunização podendo diminuir os efeitos de potenciais reações hemolíticas
Abstract: Accurate phenotyping of red blood cells (RBCs) can be difficult in transfusiondependent patients such as those with thalassemia and sickle celIs anemia because of the presence of previously transfused RBCs in the patient's circulation. Recently,the molecular basis associated with the expression of many blood group antigens was established. This allowed the development of a plethora of polymerase chain reaction (PCR)-based tests for identification of the blood group antigens by testing DNA. These new technologies complement phenotyping by overcoming some of the limitations of hemagglutination assays. These molecular assays were developed on the basis of DNA sequences of individuals of Caucasian ancestry. The present study addresses the concern that these genotyping assays may not be suitable to populations of highly diverse ancestry because of variability in intronic regions or because of unrecognized alleles. We determined both, phenotype and genotype for RHD, RH C/c, RH E/e, KEL 1/KEL 2, JK A/JK B, FY A/ FY B (nt -33) in 250 normal blood donors using PCR. Phenotype and genotype results agreed in 100% of the cases, indicating that molecular genotyping protocols can be effectively applied to populations with a highly diverse genetic background. Furthermore" genotyping for Duffy antigens provided information that could not be obtained by phenotyping. Essentially, 30.5 % ofthe donors with the FY B gene typed as Fy(b-) due to mutation in the GATA box. This information is very useful for the management of transfusion dependent patients. We also evaluated the usefulness of DNA genotyping for red blood cell antigens as a tool for the management of polytransfused patients with 'beta' thalassemia and Sickle Cell disease (SCD) to overcome the limitations of hemagglutination assays. We studied blood samples ftom 10 patients with 'beta' thalassemia and 40 SCD patients by hemagglutination and by Polymerase Chain Reaction/Restriction Fragment Length Polymorphism (pCR-RFLP) for the Rh (D, C/c, E/e), Kell, Kidd and Duffy systems. We found discrepancies between hemagglutination and DNA typing test results in nine of the ten patients with 'beta' thalassemia (90%) and in six of the forty SCD patients (15%). In these 15 patients, DNA typing results by PCR-RFLP from buccal cells were identical with those from peripheral blood leukocytes, ruling out a potential role for microchimerism due to contaminating donor leukocytes. DNA typing of samples from segments of transfused units confinned the blood group phenotype of blood donors to these patients. In conclusion, DNA typing of blood groups by PCR-RFLP in peripheral blood leukocytes contributes to the man~gement of transfusions in patients with 'beta' thalassemia and in SCD patients by allowing more accurate selection of donor units with consequent prevention of alloimmunizationand potential hemolytic reactions
Doutorado
Clinica Medica
Doutor em Clínica Médica
Stephenson, Elizabeth. "Possible Limits to Range Expansion for Non-native Asian Shore Crabs in Maine: A Biogeographic-thermogeographic Approach." Fogler Library, University of Maine, 2007. http://www.library.umaine.edu/theses/pdf/StephensonE2007.pdf.
Повний текст джерелаDonnet, Thibault. "Développement d'une nouvelle méthode de déshydratation des plaquettes sanguines par zéodratation." Paris 6, 2013. http://www.theses.fr/2013PA066033.
Повний текст джерелаGurung, Ishwori. "Deciphering type IV pilus biology in the Gram-positive opportunistic pathogen Streptococcus sanguinis." Thesis, Imperial College London, 2017. http://hdl.handle.net/10044/1/55879.
Повний текст джерелаMedina, Flores Dyanne Adenea, Urizar Gabriela Ulloa, Colarossi Rosella Camere, García Stefany Caballero, Tovalino Frank Mayta, and Valle Mendoza Juana Del. "Antibacterial activity of Bixa orellana L. (achiote) against Streptococcus mutans and Streptococcus sanguinis." Universidad Peruana de Ciencias Aplicadas (UPC), 2016. http://hdl.handle.net/10757/604437.
Повний текст джерелаPeer review
Camere, Colarossi Rosella, Urizar Gabriela Ulloa, Flores Dyanne Medina, García Stefany Caballero, Tovalino Frank Mayta, and Valle Mendoza Juana Mercedes Del. "Antibacterial activity of Myrciaria dubia (Camu camu) against Streptococcus mutans and Streptococcus sanguinis." Elsevier B.V, 2016. http://hdl.handle.net/10757/620656.
Повний текст джерелаThis study was supported by Universidad Peruana de Ciencias Aplicadas (UPC) Lima-Peru with Grant No. MANUSCRIPT ACCEPTED ACCEPTED MANUSCRIPT UPC-501-2015
Oliveira, Eduardo Soares de. "SEMEN SANGUINIS CRISTIANORUM: A CONSTRUÇÃO DE UM PROJETO DE IDENTIDADE CRISTÃ EM TERTULIANO." Pontifícia Universidade Católica de Goiás, 2014. http://localhost:8080/tede/handle/tede/771.
Повний текст джерелаEsta tese apresenta o apologista Tertuliano de Cartago e seu projeto, que busca defender o cristianismo diante das arbitrariedades do Império Romano e sua consequente e injusta perseguição. O principal tema desta pesquisa é o martírio e tem como principal objeto os textos arrolados de Tertuliano. O presente trabalho tem como objetivo e problemática principal demonstrar a importância do martírio no cristianismo africano durante o período imperial romano e sua função na construção de um projeto identitário para o cristianismo. É nesse momento que se tem na região africana do Império as condições que favoreceram o crescimento do incipiente movimento cristão que buscava se afirmar na África romanizada. Dentre a vasta obra do autor, em que se apresenta o seu posicionamento político favorável aos cristãos, várias destas obras se destacam, são elas Apologeticum, Ad Martyras, Scorpiace, Ad Scapulam. Para tanto, esta pesquisa está estruturada em três capítulos. No primeiro capítulo, busca-se identificar como se apresenta o cristianismo na transição do segundo para o terceiro século d.C., destacando-se o domínio de Roma sobre a África. No segundo capítulo, analisa-se o martírio e suas características e perspectivas dentre as quais se identifica o papel das perseguições como fundamentais neste processo. Chamam a atenção para o sacrifício como representação identitária e a conformação da memória cristã martirial a partir da literatura cristã latina, enquanto ponto fundamental e simbólico do momento martirial. Já no terceiro capítulo, esta postura martirial cristã é vista como consolidadora do movimento cristão na África. Ao se ter em vista que os mártires se tornem exemplum de fé e compromisso para com o evangelho, percebe-se que o sacrifício cristão, a partir do martírio, se afirma enquanto projeto identitário deste cristianismo africano latino-romano.
Nail, Sandrine. "L'interaction plaquettes sanguines-levures in vivo et ses conséquences." Angers, 2002. http://www.theses.fr/2002ANGE0505.
Повний текст джерелаEssassi, Dalila. "Liaisons sanguines, transferts cérébral et hépatique de la pipequaline." Paris 12, 1991. http://www.theses.fr/1991PA120008.
Повний текст джерелаVIAL, CATHERINE. "Les recepteurs p2 des plaquettes sanguines : identification et caracterisation." Université Louis Pasteur (Strasbourg) (1971-2008), 1998. http://www.theses.fr/1998STR13223.
Повний текст джерелаRice, Alison Mary. "Caractérisation fonctionnelle des cellules souches sanguines mobilisées par chimiothérapie." Bordeaux 2, 1993. http://www.theses.fr/1993BOR28240.
Повний текст джерелаRandriantsoa, Mialy. "Synthèse microbiologique des antigènes glucidiques des groupes sanguins." Université Joseph Fourier (Grenoble), 2008. http://www.theses.fr/2008GRE10132.
Повний текст джерелаSaccharidic blood group antigens are well known for their crucial role in blood transfusion and organe transplantation. They were first discovered more than a century ago on red cells and were later found in various tissues and secretion fluids. The rise of glycobiology during the past twenty years demonstrates their involvment in other biological mechanisms such as the binding of bacteria, toxins, or viruses to mammalian cell surface glycans or the specific role in oncogenesis and embryogenesis. From these different implications derived several therapeutic applications, the blood group antigens are very promising targets for drug development. In this perspective, large amount of these molecules is required. Chemical and enzymatic syntheses are developed but not allow the obtaining of large scale preparative samples of pure well-characterized oligosaccharides for use in biological studies. An alternative approach called “the living factory” is proposed in this study, it is based on high cell density culture of Escherichia coli strain overexpressing the glycosyltransferase genes. Through this process, some fifty oligosaccharides with biological interest belong to three systems of blood group (ABH type 1, 2, 4, 5, Lewis and P) have been synthesized in gram scale
Souza, Filho Wanderley de. "Determinação de parametros de forma para analise de celulas sanguineas." [s.n.], 2000. http://repositorio.unicamp.br/jspui/handle/REPOSIP/276530.
Повний текст джерелаDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Computação
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Resumo: Este traba1ho foi realizado juntamente com o Laboratório de Anatomia Patológica UNICAMP na análise de AgNORs (Regiões Organizadoras do Nuc1éolo) em células sangüíneas. Estudos anteriores demonstram que o padrão das AgNORs pode evidenciar alterações de proliferação e diferenciação celular. Assim, o estudo das AgNORs poderia dar informações adicionais sobre a cinética celular. Na grande maioria das pesquisas, a análise de AgNORs é restrita a parâmetros simples, como o número de precipitações ou a área ocupada pelas AgNORs. O objetivo deste trabalho é introduzir novos parâmetros de forma, ainda não considerados na literatura, que possam trazer informações objetivas adicionais para melhorar a classificação entre vários tipos de neoplasias e, eventualmente, trazer informações prognósticas complementares. Para tanto, foi desenvolvido um sistema ImAgNOR - com ferramentas que possibilitam tanto a segmentação semi-automática das estruturas como a extração dos parâmetros relacionados
Abstract: This work was accomplished together with the Laboratory of Pathological Anatomy UNICAMP in the analysis of AgNORs (Nucleolar Organizing Regions) in blood cells. Previous studies demonstrate that the pattem of AgNORs can evidence proliferation alterations and cellular differentiation. Thus, the study of AgNORs could give additional information on the cellular kinetics. In many researches, the analysis of AgNORs is restricted to simple parameters, as the number of precipitations or the area occupied by AgNORs. The goal ofthis work is to introduce new shape parameters, not yet considered in the literature that can bring additional objective information to improve the c1assification among several types of neoplasias and, eventually, to bring supplementary prognostic information. In this sense, a system was developed - ImAgNOR - with tools that facilitate the semiautomatic segmentation of the structures as well as the extraction of the related parameters
Mestrado
Mestre em Ciência da Computação
Credidio, Edson Velardi. "Estudo do efeito do abacate nos lipideos sanguineos em humanos." [s.n.], 2010. http://repositorio.unicamp.br/jspui/handle/REPOSIP/256668.
Повний текст джерелаTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
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Resumo: O consumo regular de abacate Persea gratissima, tipo ¿Avocado¿, frutos que incluem as espécies Fuerte e Hass, foi avaliado em relação ao efeito sobre os lipídeos plasmáticos em humanos. Foi realizado estudo prospectivo, com intervenção. Foram selecionados voluntários militares, da ativa do Batalhão de Policia Militar de São Paulo em Campinas - Unidade de Saúde do CPI - 2 de Campinas, São Paulo. A escolha desta população alvo, foi a possíbilidade da utilização, de dieta e condicionamento físico uniformes e comparáveis. Os indivíduos selecionados, após assinatura Termo de Consentimento Livre e Esclarecido ¿ TCLE tiveram o sangue coletado para dosagem dos níveis de colesterol total e frações e de triglicérides, no início e após o consumo do abacate no término do experimento. Foram estimados os valores de Colesterol plasmático (HDL- LDL- VLDL) e Triglicérides , após a utilização da polpa da fruta do abacate por um período de dois meses tendo a finalidade de determinar o efeito do consumo do abacate nos lipídeos sanguíneos. Foi observado após o tempo de intervenção houve redução dos índices lipemicos com redução dos índices lipêmicos sanguíneos e significativo aumento do Colesterol ¿ HDL em 99% dos militares participantes. O grupo controle foi constituído pelos militares que mantiveram a mesma rotina dos voluntários, porém não ingeriram o abacate. Foram analisadas também a composição da polpa do fruto visando a melhor compreensão dos resultados obtidos na pesquisa
Abstract: The regular use of the Persea gratissima ¿Avocado¿ kind, fruits that include the Fuerte and Hass varieties, was evaluated in relation to the effects on the plasmatic lipids in human. A prospective study was realized with intervention. Soldiery volunteers in active from the battalion of São Paulo military police in Campinas ¿ CPI Health unit of Campinas, São Paulo. The choice of this specific group was the possibility to have an equal and comparable diet and physical condition. After the subscription of the informed consent ¿ IC, the select group had their blood collected for total cholesterol and fraction and triglycerides levels test, In the beginning and after the use of Avocado in the end of the experiment. The plasmatic (HDL ¿ LDL ¿ VLDL) cholesterol and triglycerides were obtained after the use of the pulp of Avocado for two months in order to determine the effect of the Avocado use in the blood lipids levels. It was observed, after intervention period, that there was a reduction of the blood lipemic levels and a significative HDL ¿ Cholesterol increase in 99% of the soldiery group. The control group was formed by soldiery that kept the same routine, but did not eat the Avocado. The nutritional composition of the Avocado pulp was also analyzed for a better understanding of the results, obtained in this work
Doutorado
Doutor em Ciência de Alimentos
BATISTA, Francislene Lavôr. "PRODUÇÃO DE LACASE E BIOCONVERSÃO DE FLAVONÓIDES POR Pycnoporus sanguineus." Universidade Federal de Goiás, 2009. http://repositorio.bc.ufg.br/tede/handle/tde/2130.
Повний текст джерелаThe bioconversion is an area of the biotechnology that has increased in an expansive way, and it includes enzymatic reactions by microorganisms. These microorganisms that present enzymatic systems similar to those present in mammalian systems, it s the cytochrome P450 (CYP450). That s why the biotransformation an important alternative constitute as models for drug metabolism study. The Pycnoporus sanguineus is a filamentous fungi, basidiomycete of the Polyporaceae family and laccase producer (EC 1.10.3.2), an oxidoreductase enzyme. It was evaluated the influence of flavonoids naringin, naringenin, and quercetin in the growth of P. sanguineus, production of laccase in differents culture media. It was performed various conditions of reaction, such as temperature, agitation and the time of the flavonoids addition. The bioconversion processes were carried out for 24 up to 96 hours and monitored by TLC and HPLC to confirm the existence of potential metabolites. It was used purified laccase of the Pycnoporus sanguineus to evalute the participation of laccase in metabolites of naringin production. Pycnoporus sanguineus developed in differents culture media tested. Naringenin and naringin presented the capability to induce the laccase production by P. sanguineus, whereas quercetin and rutin inhibited the laccase production. The incubation using PDSM did not producted laccase. It was detected several of metabolites, whereas the incubations using quercetin acquired fourteen differents metabolites and the incubations using naringenin producted nine metabolites and naringin producted eight metabolites. In biocatalytic assay under 28ºC and 150rpm using naringin it was not detected the presence of metabolites
A bioconversão é uma área da biotecnologia que tem crescido extensamente e inclui reações enzimáticas por meio de microrganismos. Dentre os microrganismos utilizados destacam-se os fungos filamentosos, que apresentam um sistema enzimático semelhante ao dos seres humanos, o citocromo P450 (CYP, P450), responsável pelo metabolismo dos fármacos. O Pycnoporus sanguineus é um basidiomiceto da família Polyporaceae produtor de lacase (EC 1.10.3.2), que é uma enzima da classe das oxidoredutases. Neste trabalho, foi avaliada a influência dos flavonóides naringina, naringenina e quercetina no crescimento de P. sanguineus e produção de lacase em diferentes meios de culturas. Foram utilizadas condições reacionais variadas como alteração de temperatura, agitação e o tempo de adição dos flavonóides. As reações de bioconversão de flavonóides por P. sanguineus foram realizadas por um período de 24 a 96 horas e monitoradas por CCD e CLAE para verificar a presença de possíveis metabólitos. Aplicou-se a lacase purificada do P. sanguineus para avaliar a participação desta enzima na produção dos metabólitos da naringina. O fungo cresceu nos meios de cultura testados. A naringenina e a naringina apresentaram a capacidade de induzir a produção de lacase pelo fungo, enquanto a quercetina inibiu a produção da mesma. Nas incubações com o meio PDSM não houve a produção de lacase. Nos ensaios de bioconversão foram detectados vários metabólitos nas incubações com microrganismo inteiro, sendo que nas incubações com quercetina foram obtidos catorze metabólitos diferentes. Nas incubações com naringenina foram produzidos nove metabólitos e a naringina levou à produção de oito metabólitos. No ensaio biocatalítico a 28°C e 150rpm na presença de naringina não foi evidenciada a presença de metabólitos
Martinazzo-Portz, Tatiane. "Formulação de substrato para produção de basidiocarpos de Pycnoporus sanguineus." Universidade Estadual do Oeste do Paraná, 2011. http://tede.unioeste.br:8080/tede/handle/tede/1418.
Повний текст джерелаCoordenação de Aperfeiçoamento de Pessoal de Nível Superior
The biochemical composition of fungi can vary with climatic conditions and cultivation substrate. Several species have shown biochemical activities, such as Pycnoporus sanguineus, which is highlighted by orange to red color of their basidiocarps. Although the antimicrobial activity verified in various fields such as pharmaceutical, industrial and agricultural, there is no method for growing basidiocarps described in the literature. This work aimed to develop a substrate with Eucalyptus sp. for the cultivation of P. sanguineus and production of basidiocarps. There were two assays, and in vitro and in formulated substrate, developed in the Unioeste, Campus of Marechal Cândido Rondon. We used sawdust of Eucalyptus sp. Separate in two particle sizes: less than 500 microns (G1) and between 500 and 841 microns (G2). In the in vitro assay were used four isolates of P. sanguineus (Ps04, Ps08, PS13 and PS14), grown in Petri dishes containing culture medium CBA plus sawdust in the proportions of zero, 1, 5, 10 and 15% for both sizes. In each dish was peaked a 0.5 cm disc containing mycelium and kept in dark at 25±2ºC, until the first treatment to reach the edge of the Petri dish. Was evaluated the diameter of colony, growth rate of mycelium, the fresh weight of mycelium and production of the pigment cinnabarin. The experiment was DIC in 4x2x5 factorial design, four isolates of P. sanguineus, two particle size of sawdust of Eucalyptus sp. and five concentrations of sawdust added to the culture medium, both with six repetitions. In the assay with formulated substrate, we used two isolates of P. sanguineus (Ps08 and Ps14), grown in polypropylene bags of 28x16 cm, containing sawdust of Eucalyptus sp. and rice bran. At zero, 5 and 20%, particle size in G1 and G2, with humidity of 75% and compacted to 0.5 g mL-1. The substrates remained 30 days in BOD at 25±2ºC, then taken to the greenhouse. Two havest of basidiocarps were made, at 90 and 180 days. Where were evaluated diameter, fresh and dry weight, the average number of basidiocarps and production cinnabarin. Was used experimental DIC in 2x2x3 factorial design, with two isolates of P. sanguineus, two particle size of sawdust of Eucalyptus sp. and three concentrations of rice bran added to sawdust, both with six repetitions. Was observed that the sawdust of Eucalyptus sp. is suitable for substrate for the production of basidiocarps of P. sanguineus, which is G1 showed better results only in vitro assays, while on substrate, the two particle sizes showed good results for the production isolates of P. sanguineus. The Ps08, produced more mass of basidiocarps, while the Ps14 showed higher content of cinnabarin in the basidiocarps. It can be concluded that the genetic characteristics of the biological potential of the isolates of P. sanguineus are more related to productive results that the characteristics of substrates used in this research
A composição bioquímica dos fungos pode variar com condições climáticas de cultivo e substrato. Diversas espécies têm mostrado atividades bioquímicas, como o Pycnoporus sanguineus, que se destaca pela coloração vermelho-alaranjada de seus basidiocarpos. Embora este fungo possua atividade antimicrobiana estudada em diversas áreas, como farmacêutica, industrial e agrícola, não há nenhum método para cultivo de basidiocarpos descrito na literatura. Este trabalho objetivou desenvolver substrato com resíduos madeireiros de Eucalyptus sp. para o cultivo de P. sanguineus e produção de basidiocarpos. Realizaram-se dois ensaios, in vitro e em substrato formulado, desenvolvidos na UNIOESTE, Campus de Marechal Cândido Rondon. Utilizou-se serragem de madeira de Eucalyptus sp., separada em duas granulometrias: inferior a 500 micra (G1) e entre 500 e 841 micra (G2). No ensaio in vitro utilizaram-se quatro isolados de P. sanguineus (Ps04, Ps08, Ps13 e Ps14), cultivados em placas de Petri contendo meio de cultura CBA acrescidos de serragem nas proporções de zero, 1, 5, 10 e 15%, para as duas granulometrias. Em cada placa foi repicado um disco de 0,5 cm contendo micélio e mantidos em ambiente escuro a 25±2ºC, até que o primeiro tratamento atingisse a borda da placa de Petri. Avaliou-se diâmetro de colônia, velocidade de crescimento do micélio, a massa fresca do micélio e a produção do pigmento cinabarina. O delineamento experimental foi DIC, em esquema fatorial 4x2x5, sendo quatro isolados de P. sanguineus, duas granulometrias de serragem de Eucalyptus sp. e cinco concentrações de serragem acrescidos ao meio de cultivo, ambos com seis repetições. No ensaio em substrato formulado, utilizou-se dois isolados de P. sanguineus (Ps08 e Ps14), cultivados em sacos de polipropileno de 28x16 cm, contendo serragem de Eucalyptus sp. e farelo de arroz, nas proporções de zero, 5 e 20%, nas granulometrias G1 e G2, com umidade de 75% e compactados a 0,5 g mL-1. Os substratos permaneceram 30 dias em BOD a 25±2ºC e então foram levados a casa de vegetação. Realizaram-se duas coletas de basidiocarpos, a primeira após 90 dias e a segunda após 180 dias do ensaio em casa de vegetação. A cada coleta avaliou-se o diâmetro, as massas fresca e seca, o número médio de basidiocarpos e a produção do pigmento cinabarina. Utilizou-se delineamento experimental DIC, em esquema fatorial 2x2x3, sendo dois isolados de P. sanguineus, duas granulometrias de serragem de Eucaliptus sp. e três concentrações de farelo de arroz adicionadas a serragem, ambos com seis repetições. Foi observado que a serragem de Eucalyptus sp. é apropriada para formulação de substrato para produção de basidiocarpos de P. sanguineus, sendo a que G1 mostrou melhores resultados apenas nos ensaios in vitro. Nos ensaios em substrato formulado, as duas granulometrias mostraram bons resultados produtivos para os isolados de P. sanguineus. O Ps08, nos ensaios em substrato formulado, teve produção de massa de basidiocarpos, enquanto o Ps14 mostrou maior teor de cinabarina nos basidiocarpos. Pode-se concluir que as características genéticas de potencial biológico dos isolados de P. sanguineus estão mais relacionados aos resultados produtivos que as características dos substratos utilizados nesta pesquisa
PAIVA, JÚNIOR Sérgio de Sá Leitão. "Detecção de vasos sanguineos em imagens de fundo de olhos." Universidade Federal Rural de Pernambuco, 2006. http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5311.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
We describe an automated method for blood vessel segmentation in two-dimensional color retinal images. This method may be used by specialized software for automated screening of diabetic rethinopaty and other eye diseases, for evaluation of treatment evolution, and clinical study. The proposed method is based on a combination of “2d matched filter” response procedure (for vessel location), and the Hoshen-Kopelman algorithm (for vessel segmentation). We compare the results obtained through this method with the results of manual segmentation of 20 imagens of the DRIVE project, where ROC(Receiver Operating Characteristic curve) analysis was used for quantitative comparison with others works published on this subject.
Descrevemos neste trabalho um método automático para localizar e segmentar vasos sanguíneos em imagens de fundo de olhos. Este método pode ser usado por um software especialista em imagens de retina para diagnosticar automaticamente doenças como retinopatia diabética entre outra s , para avaliação da evolução do tratamento, e para estudos clínicos. O método proposto é baseado na combinação do procedimento “2d matched fi l t e r” ( usado na localização dos vasos) com o algoritmo de “Hoshen-Kopelman” (usado na segmentação dos vasos). Resultados obtidos com este método foram comparados, com a segmentação manual em 20 imagens do projeto DRIVE,usando ROC(Receiver Operating Characterist i c curve) para comparação quant i t a t i v a com os resultados de outros trabalhos publicados sobre este assunto.