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Добірка наукової літератури з теми "Salivary peptide"

Автор: Grafiati
Опубліковано: 11 березня 2023

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Статті в журналах з теми "Salivary peptide"

1

Ito, Seiki, Toshimitsu Suzuki, Takeshi Momotsu, Satoko Isemura, Eiichi Saitoh, Kazuo Sanada, and Akira Shibata. "Presence of salivary Protein C and salivary peptide P-C-like immunoreactivity in the laryngo-tracheo-bronchial glands." Acta Endocrinologica 108, no. 1 (January 1985): 130–34. http://dx.doi.org/10.1530/acta.0.1080130.

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Abstract. An indirect immunofluorescence technique using antisera aganist salivary peptide P-C and against salivary Protein C was carried out on the laryngeal, tracheal and bronchial glands to examine whether salivary peptide P-C-like immunoreactivity, recently demonstrated in the serous cells of the human salivary glands, was also present in those of laryngeal, tracheal and bronchial glands and to ascertain whether salivary peptide P-C is a fragment of salivary Protein C or not. Salivary peptide P-C-like immunoreactivity was present in the serous cells of the human laryngeal, tracheal and bronchial glands. Observation of serial sections immunostained with two kinds of antisera revealed that cells reacting with antisera against salivary peptide P-C were identical to those reacting with antisera against salivary Protein C pre-incubated with salivary peptide P-C. The finding implied that salivary peptide P-C and salivary Protein C, originally isolated from human saliva, were also present in the serous cells of tissues other than the salivary glands. Furthermore, analysis of the primary structure of salivary peptide P-C and salivary Protein C together with the present morphological finding suggests that salivary peptide P-C is a COOH-terminal fragment of salivary Protein C. Thus, salivary Protein C and salivary peptide P-C may play some role in the function of the serous cells of the salivary and laryngo-tracheobronchial glands.
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2

Nicolodi, Maria, and Elena Del Bianco. "Sensory Neuropeptides (Substance P, Calcitonin Gene-Related Peptide) and Vasoactive Intestinal Polypeptide in Human Saliva: Their Pattern in Migraine and Cluster Headache." Cephalalgia 10, no. 1 (February 1990): 39–50. http://dx.doi.org/10.1046/j.1468-2982.1990.1001039.x.

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Substance P, calcitonin gene-related peptide and vasoactive intestinal polypeptide-like immunoreactivities have been evaluated in the saliva of 15 subjects suffering from migraine without aura and 16 control subjects. All three peptides were also measured in the symptomatic/non-symptomatic side saliva sampled from 10 cluster headache sufferers during the cluster period, 5 cluster headache sufferers out of the cluster period, as well as in the right and left side saliva of 18 control subjects. The most interesting result gives a clear difference in common migraine and cluster headache salivary vasoactive intestinal polypeptide-like immunoreactivity contents. In fact, these are enhanced during cluster headache attack and decreased during migraine attack when compared with the interictal period vasoactive intestinal polypeptide-like immunoreactivity levels. Another remarkable finding concerns the significant increase of substance P-like immunoreactivity and calcitonin gene-related peptide-like immunoreactivity levels, from basal values, in the saliva sampled during both migraine and cluster headache attacks. Control subjects showed a calcitonin gene-related peptide-like immunoreactivity and substance P-like immunoreactivity salivary contents significantly higher than migraine sufferers' saliva sampled in basal conditions. Conversely, calcitonin gene-related peptide-like immunoreactivities levels in controls were lower than in cluster headache sufferers' saliva obtained during intervals. Finally, during cluster headache attacks the enhancement of substance P-like immunoreactivity and vasoactive intestinal polypeptide-like immunoreactivity salivary contents interest the non-symptomatic side, whereas the symptomatic side salivary substance P-like immunoreactivity and vasoactive intestinal poly-peptide-like immunoreactivity contents remain unchanged. These findings do not allow any final conclusion. However, this biochemical evaluation indicates relevant changes of the salivary neuropeptides in diseases, such as migraine and cluster headache, in which pain transmission is surely involved.
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3

Ito, Seiki, Toshimitsu Suzuki, Tooru Izumi, Takeshi Momotsu, Satoko Isemura, Eiichi Saitoh, Kazuo Sanada, and Akira Shibata. "Intracellular localization of salivary peptide P-C-like immunoreactivity in the human pancreatic B-cells." Acta Endocrinologica 108, no. 1 (January 1985): 119–29. http://dx.doi.org/10.1530/acta.0.1080119.

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Abstract. In order to clarify the intracellular localization of salivary peptide P-C-like immunoreactivity in human pancreatic B-cells, an immunohistochemical study at electron microscopic levels was carried out by the protein A-gold technique using antisera against insulin and salivary peptide P-C. Both salivary peptide P-C-like immunoreactivity and insulin-like immunoreactivity were present only in the insulin secretory granules of the pancreatic B-cells. However, the former immunoreactivity was lacking in many insulin secretory granules of foetal pancreatic B-cells while the latter immunoreactivity was seen in all insulin secretory granules. Salivary peptide P-C-like immunoreactivity was not found in the other kinds of cells in the islets. In a previous immunohistochemical study at light microscopic level, salivary peptide P-C-like immunoreactivity appeared in a few pancreatic B-cells at about the 16th week of gestation, in an increasing number during gestation, and was seen in all pancreatic B-cells a few months after birth. The present finding together with the above results suggest that absence of salivary peptide P-C-like immunoreactivity in some foetal pancreatic B-cells may be due to the underdevelopment of salivary peptide P-C-like immunoreactivity in each insulin secretory granule. From the examination of cross-reactivity of antisera against salivary peptide P-C to other kinds of salivary peptides and salivary Protein C, and from the results of an indirect immunofluorescence technique using three kinds of antisera including antisera against salivary peptide P-C, salivary peptide P-B and salivary Protein C, it was thought that salivary peptide P-C-like immunoreactivity in human pancreatic B-cells belongs neither to salivary Protein C nor to salivary peptide P-B nor to salivary peptide P-E, but either to salivary peptide P-C itself or to an unknown substance which has common antigenic determinants with salivary peptide P-C, salivary peptide P-B and salivary Protein C. Salivary peptide P-C-like immunoreactivity was not found in the pancreatic B-cells of other mammals. Thus, although a new substance other than insulin is present in the insulin secretory granules of the human pancreatic B-cells, its pathophysiological function remains unclear.
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4

Veenstra, Jan A. "The salivary gland salivation stimulating peptide from Locusta migratoria (Lom-SG-SASP) is not a typical neuropeptide." PeerJ 5 (July 26, 2017): e3619. http://dx.doi.org/10.7717/peerj.3619.

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The salivary gland salivation stimulating peptide was identified from the salivary glands of the migratory locust by its ability to stimulate cAMP production in the same tissue. The gene coding for this peptide has recently been identified and been shown to code for a precursor consisting of a signal peptide, several copies of the peptide separated by Lys–Arg doublets and a few other peptides. These data are consistent with it being a neuropeptide. However, antiserum raised to this peptide labels the acini of the salivary glands while RT-PCR only gives positive results in the salivary gland, but not in any ganglion of the central nervous system. Thus, this peptide is not a typical neuropeptide as previously assumed.
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Morris, Katherine E., Chris D. St. Laurent, Ryan S. Hoeve, Jim Wickware, Paul Forsythe, Ron Mathison, and A. Dean Befus. "The sympathetic nervous system regulates the release of anti-inflammatory peptides from salivary glands (93.18)." Journal of Immunology 182, no. 1_Supplement (April 1, 2009): 93.18. http://dx.doi.org/10.4049/jimmunol.182.supp.93.18.

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Abstract Chronic and acute stress have profound effects on inflammation. In rats, allergic inflammation is regulated by the sympathetic nervous system acting on salivary glands. Human asthma is frequently accompanied by salivary gland inflammation. Salivary gland dysfunction in stressed individuals could enhance asthma severity. Salivary gland prohormone SMR1 (submandibular rat-1) is cleaved into two peptides that are anti-inflammatory in rats, mice, dogs, sheep, cats, and human cells in pulmonary inflammation, food allergy, septic shock, pancreatitis, and spinal cord injury. We hypothesized that modulation of the autonomic nervous system would change the expression, processing, and secretion of SMR1 and its peptides. Rats were injected with saline, isoproterenol, or pilocarpine, or the superior cervical ganglion was excised. Saliva, blood, and tissues were collected and analyzed for SMR1. Adrenergic stimulation caused the majority of SMR1 into be secreted into saliva in 60 min. Removal of the superior cervical ganglion that innervates the salivary glands changed SMR1 protein levels in the salivary glands. SMR1 secretion into saliva in response to acute stress may provide a large pool of SMR1-derived peptide products that mediate anti-inflammatory responses locally and systemically. This research is funded by AllerGen NCE.
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Yi, Ting Chun, and Shabbir Moochhala. "Mini-Review Article – Current Opinion on Salivary Biomarkers as a Measurement for Stress and Fatigue." Open Biomarkers Journal 6, no. 1 (May 17, 2013): 9–14. http://dx.doi.org/10.2174/1875318301306010009.

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Salivary biomarkers have been increasingly popular in stress research as saliva is easily produced and collection is non-invasive and not limited by geographical distance or lack of infrastructure. Several salivary biomarkers have been utilized in stress research, for instance, salivary cortisol, salivary amylase and salivary immunoglobulin A. Despite being sensitive to changes in fatigue, they have limitations such as inter-individual variability, and interactions with other constituents that may confound the results. Recently, Hyperion Biotechnology has developed the Fatigue Biomarker Index (FBI), which is a measurement of the changes in concentration of salivary peptides with fatigue. The FBI has been shown to be an accurate and objective biomarker of fatigue, and has huge potential for use in various fields and industries. This article will review some of the previous and current salivary biomarkers of stress, as well as critically appraise the new salivary peptide test in terms of its accuracy, application and access.
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7

Tao, Renchuan, Richard J. Jurevic, Kimberly K. Coulton, Marjorie T. Tsutsui, Marilyn C. Roberts, Janet R. Kimball, Norma Wells, Jeffery Berndt, and Beverly A. Dale. "Salivary Antimicrobial Peptide Expression and Dental Caries Experience in Children." Antimicrobial Agents and Chemotherapy 49, no. 9 (September 2005): 3883–88. http://dx.doi.org/10.1128/aac.49.9.3883-3888.2005.

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ABSTRACT Dental caries is a major worldwide oral disease problem in children. Although caries are known to be influenced by dietary factors, the disease results from a bacterial infection; thus, caries susceptibility may be affected by host factors such as salivary antimicrobial peptides. This study aimed to determine a possible correlation between caries prevalence in children and salivary concentrations of the antimicrobial peptides human beta-defensin-3 (hBD-3), the cathelicidin LL37, and the alpha-defensins HNP1-3 (a mixture of HNP1, 2, 3). Oral examinations were performed on 149 middle school children, and unstimulated whole saliva was collected for immunoassays of the three peptides and for assay of caries-causing bacteria in saliva. The median salivary levels of hBD-3, LL37, and HNP1-3 were in the microgram/ml range but were highly variable in the population. While levels of LL37 and hBD-3 did not correlate with caries experience, the median HNP1-3 levels were significantly higher in children with no caries than in children with caries. Children with high caries levels did not have high levels of salivary Streptococcus mutans, and the HNP1-3 level was not correlated with salivary S. mutans. By immunohistochemistry we localized HNP1-3 in submandibular salivary duct cells. HNPs are also released by neutrophils into the gingival crevicular fluid. Both sources may account for their presence in saliva. Low salivary levels of HNP1-3 may represent a biological factor that contributes to caries susceptibility. This observation could lead to new ways to screen for caries susceptibility and to new means of assessing the risk for this common oral problem.
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Yasuda, Takuya, Koichiro Tahara, and Tetsuji Sawada. "Detection of salivary citrullinated cytokeratin 13 in healthy individuals and patients with rheumatoid arthritis by proteomics analysis." PLOS ONE 17, no. 3 (March 23, 2022): e0265687. http://dx.doi.org/10.1371/journal.pone.0265687.

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The immune response to citrullinated peptides in the mucosa has been suggested to play an important role in the transition from pre-onset rheumatoid arthritis (RA) to clinically evident RA. Although there are reports indicating the presence of anti-citrullinated peptide antibodies in the saliva, few studies have reported citrullinated peptide detection in human saliva. This study aimed to identify citrullinated peptides in human saliva and discuss their clinical significance. Saliva samples were collected from 11 patients with RA and from 20 healthy individuals. Citrullinated peptides were detected using an anti-modified citrulline (AMC) antibody. Saliva from the healthy individuals was subjected to two-dimensional protein electrophoresis to isolate citrullinated peptides, which were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and mass spectrometry by peptide mass fingerprinting. The results were corroborated by immunoprecipitation (IP)-western blotting. The signal intensities of the bands precipitated with anti-cytokeratin 13 (CK13) and AMC antibodies were quantified. The signal intensity ratio of the band produced by the AMC antibody was divided by that of the band produced by the anti-CK13 antibody to calculate the citrullinated CK13 (Cit-CK13) ratio. A citrullinated peptide band corresponding to a molecular weight of approximately 50 kDa was detected in the saliva of healthy individuals, and identified as CK13 via mass spectrometry and IP-western blotting. No significant difference was observed between the salivary Cit-CK13 ratios of patients with RA and healthy participants (p = 0.605). This is the first study to show that Cit-CK13 is present in human saliva, and that there is no significant difference between the Cit-CK13 ratios of patients with RA and healthy individuals, suggesting that salivary Cit-CK13 content and RA development may not be associated. The physiological and pathological roles of Cit-CK13 in the oral cavity, and its responsiveness to mucosal immunity, remain unknown and will be the subject of further investigation.
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Ito, Seiki, Toshimitsu Suzuki, Satoko Isemura, Kazuo Sanada, Hiroyuki Anaguchi, Hirohiko Shimizu, Toshihiro Maruyama, and Akira Shibata. "'Salivary peptide P-C' of human pancreatic B-cells shares only partly immunoreactivity with salivary peptide P-C indicating a new B-cell protein which is different from insulin." Acta Endocrinologica 120, no. 1 (January 1989): 62–68. http://dx.doi.org/10.1530/acta.0.1200062.

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Abstract. Salivary peptide P-C like immunoreactivity, originally isolated from human whole saliva has later been found in the human pancreatic B-cells. In the present work an indirect immunofluorescence technique using monoclonal antibodies against isolated salivary peptide P-C was applied to Bouin fixed pancreas and parotid glands to study the possible identity of the two substances. Positive P-C immunofluroescence was found in the serous cells of parotid glands but not in pancreatic B-cells, suggesting that pancreatic P-C substance is not salivary peptide P-C itself, but a substance sharing the common antigenic site with salivary peptide P-C. To examine this, an indirect immunofluorescence technique using polyclonal P-C antisera pre-absorbed with six kinds of synthetic fragments (1–22, 23–44, 23–29, 30–44, 30–38 and 38–44) of salivary peptide P-C was applied to the human pancreas. The result showed that pancreatic P-C substance was a substance which shares the common antigenic site with the 38–44 amino acid residue of salivary peptide P-C. Western blot analysis using extracts of human pancreata further showed that pancreatic P-C substance is not a precursor of insulin but a protein with molecular weight of 11 500 dalton, indicating the presence of a new protein in the insulin secretory granules of human pancreatic B-cells.
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Hamada, Tomoyuki, Masatsugu Kawashima, Haruo Watanabe, Junji Tagami, and Hidenobu Senpuku. "Molecular Interactions of Surface Protein Peptides of Streptococcus gordonii with Human Salivary Components." Infection and Immunity 72, no. 8 (August 2004): 4819–26. http://dx.doi.org/10.1128/iai.72.8.4819-4826.2004.

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ABSTRACT Oral streptococci play a large role in dental biofilm formation, and several types interact as early colonizers with the enamel salivary pellicle to form the primary biofilm, as well as to incorporate other bacteria on tooth surfaces. Interactions of surface molecules of individual streptococci with the salivary pellicle on the tooth surface have an influence on the etiological properties of an oral biofilm. To elucidate the molecular interactions of streptococci with salivary components, binding between surface protein (SspB and PAg) peptides of Streptococcus gordonii and Streptococcus sobrinus were investigated by utilizing BIAcore biosensor technology. The analogous peptide [change of T at position 400 to K in SspB(390-402), resulting in the SspB(390-T400K-402) peptide] from S. gordonii showed the greatest response for binding to salivary components and inhibited the binding of Streptococcus sanguis by more than 50% in a competitive inhibition assay in a comparison with other SspB and PAg peptides. This peptide also bound to the high-molecular-weight protein complex of salivary components and the agglutinin (gp340/DMBT1) peptide (scavenger receptor cysteine-rich domain peptide 2 [SRCRP 2]). In addition, the SspB(390-T400K-402) peptide was visualized by two surface positive charges in connection with the positively charged residues, in which lysine was a key residue for binding. Therefore, the region containing lysine may have binding activity in S. gordonii and S. sanguis, and the SRCRP 2 region may function as a receptor for the binding. These findings may provide useful information regarding the molecular mechanism of early biofilm formation by streptococci on tooth surfaces.
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Дисертації з теми "Salivary peptide"

1

Messenger, Beatrice. "Salivary gland peptide hormones and dietary phenols." Thesis, University of Surrey, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.326511.

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2

Ribeiro, Thyciana Rodrigues. "A study of salivary peptide profile in children with early childhood caries: envisioning saliva as a diagnostic tool." Universidade Federal do CearÃ, 2009. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=3576.

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FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgico
The aim of the present study was to find a relation between salivary peptides, caries experience and mutans streptococci (MS) levels in saliva of caries free (CF) and caries susceptible (CS) children in early childhood. One hundred and six 10 â 71 month-old children participated in the study. Fifty-eight children were CF and 48 who had experienced dental caries formed the CS group. Two samples of whole saliva were collected from all participants. Unstimulated whole saliva was collected, subsequently centrifuged. Supernatants were lyophilized, divided into two pools (CF and CS) and individual samples, and stored at -20oC for posterior analysis using LC-MS (Liquid Chromatography Mass Spectrometry) to study the peptide profile. Identification of salivary peptides was based on theoretical molecular masses available from online databases. Stimulated whole saliva was collected and used for MS detection in MSB agar medium. MS concentration in saliva was reported in cfu/mL. Dental examination was performed and dmfs/dmft scores were calculated. Data was analysed by using logistic regression. The chromatograms from CF and CS pools of saliva had different peak patterns. The identification of molecular masses suggested the presence of 9 peptides. Three of them were significantly related with caries experience. The presence of HNP-3 (α-defensin 3) (p = 0.019) and HBD-3 (β-defensin 3) (p = 0.034) reduced the chances of experiencing early childhood caries (ECC). The presence of PRP IB-4 significantly increased caries experience (p = 0.035). In addition, age (p = 0.020) and MS counts (p = 0.036) increased caries experience, however gender was not associated with dental caries (p = 0.877). Our results suggest that presence of specific peptides in saliva of CF or CS children in early childhood predisposes to a higher or lower risk of caries experience.
Este trabalho buscou estudar o perfil de peptÃdeos salivares de crianÃas com cÃrie da primeira infÃncia, relacionando-o com nÃveis de estreptococos do grupo mutans (EGM) salivares e experiÃncia de cÃrie. Cento e seis crianÃas, na faixa etÃria de 10 a 71 meses de idade, participaram do estudo, sendo 48 com experiÃncia de cÃrie e 58 sem cÃrie da primeira infÃncia. Duas amostras de saliva total foram coletadas de todos os participantes. A primeira amostra era composta de saliva nÃo estimulada, utilizada para anÃlise dos peptÃdeos. ApÃs coletada, essa saliva foi centrifugada, o sobrenadante retirado, liofilizado, dividido em pools com cÃrie, sem cÃrie e em amostras individuais e armazenado em freezer a -20oC atà anÃlise em aparelho de LC-MS (Cromatografia LÃquida acoplado ao EspectrÃmetro de Massa). A busca por peptÃdeos foi baseada em massas conhecidas de peptÃdeos existentes em bancos de dados. Saliva estimulada representou a segunda coleta, utilizada para o cultivo dos EGM (UFC/mL) em meio Ãgar mitis salivarius bacitracina (MSB). Anamnese e exame dentÃrio foram realizados para cÃlculo do Ãndice ceo-s e ceo-d. Os dados foram analisados por meio de modelo logÃstico binÃrio. Resultados foram considerados significantes quando p-valor < 0,05. Os cromatogramas obtidos a partir dos pools de crianÃas com/sem cÃrie apresentaram diferenÃas em relaÃÃo aos picos apresentados. A identificaÃÃo das massas moleculares sugeriram a presenÃa de nove peptÃdeos. RegressÃo logÃstica mostrou que 3 peptÃdeos se relacionaram com experiÃncia de cÃrie. PRP IB-4 associou-se a um aumento de experiÃncia de cÃrie (p=0,035); α-defensina 3 (p=0,019) e β-defensina 3 (p=0,034) associaram-se à reduÃÃo de experiÃncia de cÃrie. Em adiÃÃo, aumento na idade (p=0,020) e aumento na contagem de EGM (p=0,036) ocasionaram um aumento na experiÃncia de cÃrie, mas sexo nÃo se relacionou com cÃrie dentÃria (p=0,877). A partir desses resultados, pÃde-se concluir que a presenÃa de peptÃdeos especÃficos na saliva de crianÃas com e sem cÃrie dentÃria predispÃem a um maior ou menor risco à essa doenÃa.
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3

DE, SANTIS Maria. "Proteomic (HLPC-ESI-MS) study of salivary peptides and proteins in patients with Sjögren's syndrome. before and after pilocarpine." Doctoral thesis, Università degli Studi di Verona, 2008. http://hdl.handle.net/11562/337582.

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OBIETTIVI. Studiare l'effetto della Pilocarpina sulla composizione proteica salivare in soggetti affetti da sindrome di Sjögren primitiva (pSS) e comparare il profilo proteico dei soggetti affetti da pSS con soggetti sani di controllo e pazienti affetti da sindrome di Sjögren secondaria (sSS). E’ stata inoltre ricercata la presenza di immunopeptidi, come le defensine e le timosine, allo scopo di descriverne l’eventuale presenza e l’abbondanza relativa nella saliva di soggetti affetti da SS e di valutarne il possibile ruolo come biomarker di malattia e/o di infiammazione. METODI. Sono stati analizzati campioni di saliva di 9 pazienti affetti da pSS, 9 sSS e 10 soggetti sani mediante High Performance Liquid Chromatography e Mass Spectrometer-Electrospray Ionization Source. In 6 pazienti con pSS sono stati raccolti campioni di saliva anche dopo 30, 60 minuti e 24 ore dall’assunzione di 5 mg di Pilocarpina. RISULTATI. Nei campioni basali, più del 50% delle proteine salivari di origine ghiandolare analizzate risultavano non rilevabili all’indagine spettrometrica o mostravano livelli significativamente più bassi nei pazienti con pSS rispetto ai soggetti sani. I pazienti con sSS mostravano un profilo di proteine salivari intermedio tra i pazienti con pSS ed i soggetti di controllo. Circa un terzo delle proteine meno rappresentate nei pazienti con pSS al basale risultavano rilevabili con frequenza simile ai controlli dopo 60 minuti dall’assunzione di Pilocarpina. Tutte le proteine con livelli significativamente più bassi al basale rispetto ai controlli raggiungevano livelli simili ai soggetti sani dopo 30 minuti dall’assunzione di Pilocarpina. La migliore risposta al farmaco è stata osservata tra le proteine di origine parotidea. I pazienti con pSS erano inoltre caratterizzati da alti livelli di α-defensina 1 e dalla presenza di β-defensina 2, peptidi di origine neutrofilica ed epiteliale rispettivamente, con funzioni antimicrobiche. Mentre la β-timosina 4, peptide strutturale con proprietà antinfiammatorie e riparatrici, risultava rilevabile in quasi tutti i soggetti sani e nei pazienti, la β-timosina 10, è stata riscontrata nella maggior parte dei pazienti con pSS e in un terzo dei soggetti con sSS; non era invece evidenziabile in nessuno dei soggetti sani. 3 CONCLUSIONI. Il nostro studio ha dimostrato come la Pilocarpina, farmaco secretagogo agonista colinergico, che si riteneva aumentare solo la quota fluida della secrezione salivare, sia invece in grado di ripristinare parzialmente i livelli ed il numero delle proteine salivari ridotte in corso di sindrome di Sjögren. L’ α-defensina 1, la β-defensina 2 e la β-timosina 10 potrebbero essere considerati biomarker di infiammazione orale nei pazienti con pSS.
Saliva is a complex fluid composed of a variety of electrolytes, metabolites, nucleotides, polynucleotides and proteins; it plays an important role in the maintenance of oral health (1). The rate of salivary protein secretion is controlled mainly by noradrenalin that is released from the sympathetic terminals and acts through the β-adrenergic receptors, while the rate of fluid and electrolyte secretion is controlled by acetylcholine, released from the parasympathetic terminals and acting through the muscarinic cholinergic receptors (2). A large number of systemic agents has been proposed as secretagogues, but only a few have shown consistent salivary secretion enhancing properties in well-designed trials. Among cholinergic agonists, pilocarpine is the most effective for protein secretion in rat (3), having also a mild β-adrenergic stimulating properties, but a few data have been reported in humans. Pilocarpine has been shown to improve symptoms of oral dryness and to increase salivary output in patients with primary Sjögren’s syndrome (pSS) (4), a chronic autoimmune disorder of the exocrine glands with associated lymphocytic infiltrates and consequent dryness of mouth and eyes (5). Saliva composition in pSS patients has been found to be different from normal subjects (6). However the pattern of salivary gland proteins in patients with pSS is not completely defined with regard to its composition, mainly in relation to low-molecular-weight components as acidic and basic proline-rich proteins (PRPs), statherins and cystatins, as well as defensins, which are immunopeptides of epithelial and neutrophilic origin, and thymosins, G-actinsequestering peptides with immuno-regulatory properties. In particular there are no data concerning the effects of pilocarpine on salivary protein profile in pSS patients. Moreover there are no studies on the possible differences in salivary protein profile between pSS and Sjögren’s syndrome associated to other rheumatic diseases (sSS).
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4

Raghunathan, Vinodhkumar. "Elucidation of molecular recognition mechanisms of a peptide involved in biomineralization using solid state nuclear magnetic resonance spectroscopy /." Thesis, Connect to this title online; UW restricted, 2006. http://hdl.handle.net/1773/8644.

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Ojha, Yagya Raj. "Selection and Characterization of ssDNA Aptamers for Salivary Peptide Histatin 3 and Their Application Towards Assay and Point-of-Care Biosensing." University of Toledo / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1575992671104993.

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Oliveira, Elaine Cyreno. "Adesão e atividade de protease são reguladas pelo peptídeo derivado da laminina AG73, sindecan-1 e integrina 1 em linhagem celular derivada de carcinoma adenóide cístico." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/42/42134/tde-09022010-105201/.

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Estudamos indução da atividade de MMP pelo peptídeo da laminina a1 AG73 em linhagem celular (CAC2) de carcinoma adenóide cístico. CAC2 cultivadas em laminina-111 com AG73 geraram espaços pseudocísticos. Inibidor de MMP diminuiu tais espaços, sugerindo ação de MMPs. CAC2 crescidas sobre AG73 mostraram aumento dose-dependente de MMP9. RNAi para MMP9 diminuiu remodelação em cultura 3D. Buscamos receptores de AG73 ligados à atividade de MMP9. CAC2 crescidas sobre AG73 exibiram colocalização de sindecan-1 e integrina b1. RNAi para sindecan-1 ou para integrina b1 geraram, isolados, redução na adesão a AG73 e nas atividades de remodelação e de protease. Duplo RNAi estudou a cooperação entre os receptores e promoveu diminuição na adesão a AG73 e na atividade de MMP. Distinção de receptores foi feita por cromatografia de afinidade e espectrometria de massa, através de colunas de afinidade com AG73 acoplado, que resultou em possíveis receptores, como integrinas b1 e aV. Sugerimos que AG73 regula adesão e secreção de MMP em células CAC2 através de sindecan-1 e integrina b1.
We studied induction of MMP activity by b1-laminin peptide AG73 in adenoid cystic carcinoma cell line (CAC2). Cells grown inside AG73-enriched laminin-111 exhibited pseudocystics spaces. MMP inhibitor decreased those spaces, suggesting MMPs action. Cells grown on AG73 showed a dose-dependent increase of MMP9 secretion. MMP9 siRNAi decreased remodeling in 3D culture. We searched for AG73 receptors regulating MMP9 activity. CAC2 grown on AG73 exhibited colocalization of syndecan-1 and b1 integrin. Syndecan-1 siRNA or siRNA b1 integrin showed reduction in adhesion to AG73 and in remodeling and protease activities. Double-knockdown explored syndecan-1 and 1 integrin cooperation and showed decrease in adhesion to AG73 and in MMP activity. Receptors characterization was made by affinity chromatography followed by mass spectrometry through AG73-affinity columns and showed putative receptors, like b1 and aV integrins. We suggest that AG73 peptide regulates adhesion and MMP secretion in CAC2 cells through syndecan-1 and b1 integrin.
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Elanga, N'Dille Clément Emmanuel. "Développement d’un biomarqueur salivaire mesurant l’exposition de l’Homme aux piqûres des moustiques Aedes : applications aux risques de transmission et à l’évaluation de l’efficacité des stratégies de lutte contre les arboviroses." Thesis, Montpellier 2, 2014. http://www.theses.fr/2014MON20023/document.

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Les infections virales transmises à l'homme par les moustiques Aedes, sont en pleine émergence ou ré-émergence dans le monde entier. Le contrôle des populations de vecteurs reste la seule méthode de lutte. Pour un contrôle plus efficace, de nombreux efforts sont déployés pour développer de nouveaux indicateurs évaluant l'exposition de l'Homme aux piqûres des Aedes. Dans ce contexte, l'objectif de la thèse était de développer un biomarqueur, basé sur l'évaluation quantitative de la réponse anticorps (Ac) spécifique au peptide salivaire Nterm-34kDa d'Ae. aegypti chez les populations exposées. Pour cela, nous avons évalué le potentiel de cette réponse Ac spécifique à i) mesurer l'intensité d'exposition aux piqûres, ii) évaluer le risque de transmission des arboviroses et iii) évaluer l'efficacité des stratégies de lutte anti-vectorielle (LAV). Nos résultats ont montré qu'une réponse IgG anti-peptide Nterm-34kDa pouvait être détectée chez les individus exposés à Ae. aegypti et Ae. albopictus. Le niveau de cette réponse IgG spécifique augmentait entre les saisons de faibles densités de moustiques et celles de fortes densités, indiquant que ce candidat biomarqueur permettrait d'évaluer l'exposition aux piqûres des Aedes. La distribution spatiale similaire de la prévalence de nouvelles infections au virus de la dengue et la prévalence de la réponse IgG spécifique montrait également que ce candidat biomarqueur permettrait d'identifier des zones à risque de transmission. La comparaison des réponses IgG anti-peptide Nterm-34kDa avant et après les interventions de LAV, a montré qu'une baisse post-LAV de cette réponse Ac spécifique permettrait d'évaluer l'efficacité de la LAV contre les Aede. Ce biomarqueur salivaire pourrait donc représenter un indicateur de la réduction du contact homme-vecteur. L'ensemble de ces travaux montre que la réponse Ac spécifique au peptide salivaire Nterm-34kDa constitue un pertinent biomarqueur pour évaluer l'exposition de l'Homme aux vecteurs des arboviroses. La validation supplémentaire de ce biomarqueur et son développement sous forme de test rapide permettraient aux structures en charge de la surveillance des arboviroses et de la lutte anti-vectorielle, de disposer d'un outil complémentaire des indicateurs entomologiques et épidémiologiques de référence
Human viral infections transmitted by Aedes mosquitoes are rapidly emerging or re-emerging worldwide. Vector control strategies remain currently the unique method to control these infections. To improve the effectiveness of this control, much effort is being devoted to develop new indicators for measuring the human exposure to Aedes bites. In this way, this project aimed to develop a biomarker based on the quantitative assessment of antibody response (Ab) to Ae. aegypti Nterm - 34kDa salivary peptide, in human exposed populations. We evaluated thus the potential of this specific Ab response to: i) measure the intensity of human exposure to Aedes bites, ii) assess the risk of transmission of arboviruses and iii) evaluate the efficacy of vector control strategies. Our results showed that a specific IgG response to Nterm-34kDa peptide could be detected in individuals exposed to Ae. aegypti or Ae. albopictus. The level of specific IgG response increased from the season of low mosquito densities to high densities one, indicating that this biomarker candidate could evaluate the intensity of exposure to the Aedes bites. The observed similar spatial distribution of the prevalence of new infections with dengue virus and specific IgG response showed that this biomarker candidate could identify areas at risk of transmission. The comparison of the specific IgG responses to Nterm-34kDa peptide before and after the vector control intervention showed a decline of the specific Ab response after implementation of control. It indicated that such salivary biomarker could assess the effectiveness of vector control against Aedes, and that this salivary biomarker could be an indicator of the reduction of man-vector contact. Altogether, the results of this work show that the specific IgG response to the Nterm-34kDa salivary peptide could be a relevant biomarker for assessing human exposure to arboviruses vectors. This promising indicator, developed as a rapid test, could represent a complementary tool for entomological and epidemiological surveillance of arboviruses diseases
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Pereira, Patrícia de Sousa. "Characterization of mammal salivary peptides." Master's thesis, Universidade de Aveiro, 2012. http://hdl.handle.net/10773/10135.

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Mestrado em Bioquímica
A saliva e os seus componentes desempenham diversas funções na cavidade oral, tais como lubrificação, proteção dos tecidos orais e ação antimicrobiana. Entre os componentes responsáveis por esses papéis estão diversos péptidos cuja evolução e presença na saliva de outras espécies de mamíferos não está clara. No presente trabalho, duas classes destes péptidos, as cistatinas salivares e a timosina β4, foram analisadas usando ferramentas de genómica e de proteómica em conjunto. Para os estudos de proteómica foi colhida saliva de cão, rato, coelho e cordeiro, sendo a separação dos péptidos presentes feita por cromatografia liquida e a análise por espectrometria de massa tandem. Para os estudos de genómica foram pesquizadas bases de dados de sequências nucleotídicas e realizaram-se análises evolutivas. No que diz respeito à timosina β4 demonstrou que este péptido apresenta uma elevada conservação entre as diferentes espécies de mamíferos. Utilizando as sequências deste péptido encontradas no genoma dos diferentes mamíferos, foi possível identificar pela primeira vez por espectrometria de massa a timosina β4 na saliva do cão. No caso da classe das cistatinas, nomeadamente cistatinas C, D e tipo-S (S, SA e SN), a análise evolutiva permitiu verificar que as cistatinas D e tipo-S são específicas dos primatas, o que sugere que terão emergindo após a grande separação dos mamíferos que ocorreu há cerca de 80-90 milhões de anos. Os resultados permitiram também verificar que algumas sequências presentes nas bases de dados encontram-se mal anotadas, incluindo a sequência atribuída à cistatina S encontrada no rato. Por outro lado, a análise filogenética demonstrou que a cistatina C está distribuída por várias classes de mamíferos. No entanto, permanece por compreender o mecanismo da sua secreção na saliva humana e a sua ausência na saliva de outras espécies de mamíferos. Em conclusão, através da combinação da proteómica e filogenia podemos caracterizar e compreender a distribuição dos péptidos salivares em diferentes mamíferos e comparar com toda a informação existente para a saliva humana.
Saliva and its components play several roles in the oral cavity, such as lubrication, protection of tissues and antimicrobial action. Among the components responsible for these roles are several peptides, which evolution and presence in other mammals’ saliva is not clear. In the present study, two peptide classes, salivary cystatins and thymosin β4, were analyzed using a combination of genomic and proteomic tools aiming the enlightening changes in the structure and distribution of these peptides between the different mammal species. For the proteomic analysis, saliva was collected from dog, rat, rabbit and lamb, being salivary peptides separated by chromatography and analyzed by tandem mass spectrometry. For the genomic studies, database of nucleotide sequences were searched and evolutionary analyses were performed. Regarding thymosin β4, the evolutionary analysis showed that this peptide is highly conserved through the collection of all peptide sequences from different mammals species genome, it was possible to identify for the first time by mass spectrometry the thymosin β4 in dog’ saliva. Respecting cystatins class, namely C, D and S-type cystatins (S, SA and SN), evolutionary analysis showed that D and S-type cystatins are Primate specific, which suggesting that these classes emerged after the great mammalian radiation at 80-90 million years ago. The results also showed errors in the annotation of these sequences in databases, in particular the sequence attributed to cystatin S detected in rat. In contrast, evolutionary analysis showed that cystatin C is widely distributed in several mammal classes. However, it is not clear their secretion mechanism to saliva and why its absence in saliva of other mammal’ species. In conclusion, using phylogenetic and proteomic approaches it will be possible to understand and characterize the distribution of these peptides in different mammal species and compare with what is known in the human saliva.
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Amaral, Ãrico Sucupira. "Ãnalise do perfil de proteÃnas salivares de crianÃas com sobrepeso e obesidade do instituto da primeira infÃncia â iprede no estado cearÃ." Universidade Federal do CearÃ, 2015. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14667.

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A obesidade à um tema recorrente na literatura cientÃfica da atualidade. Isso se deve ao aumento exponencial de sua prevalÃncia em todas as camadas da sociedade. A popularidade deste tema fez tambÃm com que assuntos associados a ele emergissem e ganhassem maior notabilidade em publicaÃÃes da Ãrea da saÃde. O uso da saliva como mÃtodo diagnÃstico avanÃou consideravelmente nos Ãltimos anos. DesequilÃbrios na quantidade e na qualidade da saliva podem tanto gerar afecÃÃes bucais quanto ser indicativo de alguma alteraÃÃo sistÃmica importante. Este trabalho objetivou estudar o perfil de proteÃnas salivar e saliva total humana em pacientes com sobrepeso e obesidade. A amostra foi constituÃda por sessenta pacientes com obesidade e sobrepeso (grupo experimental) e sessenta pacientes com peso adequado (grupo controle), tendo sido avaliado o fluxo salivar, o diÃrio de dieta e o perfil proteico. Saliva total nÃo estimulada foi coletada e armazenada a â 80ÂC. Posteriormente foi adicionado o inibidor enzimÃtico e as amostras foram centrifugadas a 15.000 rpm por 15 minutos a 4ÂC, sendo o sobrenadante separado para realizaÃÃo da dosagem de proteÃnas. A concentraÃÃo de proteÃnas totais salivares foi determinada pelo mÃtodo do Ãcido BicinconÃnico, usando uma curva de albumina sÃrica bovina (BSA). Ao analisar o fluxo salivar nÃo estimulado foi possÃvel observar que o grupo de estudo apresentou mÃdia menor que o grupo controle, sendo essa diferenÃa estatisticamente significante (p=0,006). O grupo controle apresentou uma mÃdia de concentraÃÃo total de proteÃnas maior que o grupo experimental, sendo essa diferenÃa estatisticamente significante (p=0,002). Os resultados deste estudo sugerem haver padrÃes diferenciados na composiÃÃo salivar entre os grupos avaliados.
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Larsson, Olof. "Peptides as cotransmitters in salivary secretion histochemical, biochemical and functional studies of parotid and submandibular glands /." Stockholm : Kongl. Carolinska Medico Chirurgiska Institutet, 1989. http://catalog.hathitrust.org/api/volumes/oclc/19412146.html.

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Книги з теми "Salivary peptide"

1

Larsson, Olof. Peptides as cotransmitters in salivary secretion: Histochemical, biochemical and functional studies of parotid and submandibular glands. Stockholm: Kongl. Carolinska Medico Chirurgiska Institutet, 1989.

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Larsson, Olof. Peptides as cotransmitters in salivary secretion: Histochemical, biochemical and functional studies of parotid and submandibular glands. [S.l: s.n.], 1989.

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Частини книг з теми "Salivary peptide"

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Hof, Wim van ’t, Menno J. Oudhoff, and Enno C. I. Veerman. "Histatins: Multifunctional Salivary Antimicrobial Peptides." In Antimicrobial Peptides and Innate Immunity, 167–81. Basel: Springer Basel, 2012. http://dx.doi.org/10.1007/978-3-0348-0541-4_7.

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Strasburger, C. J., C. Kirschbaum, C. Becker-Carus, W. G. Wood, and D. H. Hellhammer. "Luminescent Immunoassay for Salivary Cortisol Measurement in Psychoendocrine Studies." In Neurobiology of Amino Acids, Peptides and Trophic Factors, 257–60. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4613-1721-0_26.

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Laakso, Maija-Liisa, Tarja Porkka-Heiskanen, Dag Stenberg, and Aino Alila. "Interindividual Differences in the Responses of Serum and Salivary Melatonin to Light." In Role of Melatonin and Pineal Peptides in Neuroimmunomodulation, 307–11. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4615-3756-4_35.

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Castagnola, Massimo, Irene Messana, Tiziana Cabras, Federica Iavarone, Chiara Fanali, Anna Maria Pecoraro, Alessandra Morelli, Giovanni Neri, Maria Giulia Torrioli, and Fiorella Gurrieri. "Hypo-Phosphorylation of Salivary Peptidome as Indicator of Molecular Pathogenesis of Autism Spectrum Disorders." In Comprehensive Guide to Autism, 1543–63. New York, NY: Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4614-4788-7_87.

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Trindade, Fábio, Inês Falcão-Pires, Adelino Leite-Moreira, Pedro S. Gomes, Julie Klein, Rita Ferreira, and Rui Vitorino. "EndoProteoFASP as a Tool to Unveil the Peptidome-Protease Profile: Application to Salivary Diagnostics." In Methods in Molecular Biology, 293–310. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7537-2_19.

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Bergmeier, L. A., T. Lehner, and J. Haron. "Local oral immunisation with synthetic peptides induces a dual mucosal IgG and salivary IgA antibody response and prevents colonisation of Streptococcus mutans." In Advances in Mucosal Immunology, 336–39. Dordrecht: Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-009-1848-1_91.

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Davison, J. S., D. Befus, and R. Mathison. "Salivary gland peptides: their role in anaphylaxis and lipopolysaccharide (LPS)-induced inflammation." In NeuroImmune Biology, 307–11. Elsevier, 2001. http://dx.doi.org/10.1016/s1567-7443(01)80027-2.

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POLLOCK, J. J., B. J. MacKAY, L. DENEPITIYA, V. J. IACONO, and R. P. RENNER. "Anti-Candida Properties of Natural Salivary Histidine-Rich and Synthetic Histidine Peptides: Relevance to Denture Stomatitis." In Protides of the Biological Fluids, 309–14. Elsevier, 1985. http://dx.doi.org/10.1016/b978-0-08-031739-7.50079-3.

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Тези доповідей конференцій з теми "Salivary peptide"

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Ki-Bong Song, Chang-Bum Kim, and Yo-Han Choi. "Sensing and quantification of salivary beta-amyloid peptides and protein sequencing for the saliva of normal and AD patients." In 2015 IEEE Sensors. IEEE, 2015. http://dx.doi.org/10.1109/icsens.2015.7370592.

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Svärd, Anna, Stefan Renvert, Johan Berglund, and Maria Söderlin. "AB0143 PERIODONTITIS AND SALIVA ANTIBODIES TO CITRULLINATED PEPTIDES IN RHEUMATOID ARTHRITIS." In Annual European Congress of Rheumatology, EULAR 2019, Madrid, 12–15 June 2019. BMJ Publishing Group Ltd and European League Against Rheumatism, 2019. http://dx.doi.org/10.1136/annrheumdis-2019-eular.1209.

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Xibillé-Friedmann, D.-X., J.-I. Martínez-Rivera, M.-A. la Garza-Ramos De, J. González-Christen, S.-M. Carrillo-Vázquez, and J.-L. Montiel-Hernandez. "FRI0052 Salivary peptidyl-arginine deiminase and tannerella forsythia are associated with clinical activity of rheumatoid arthritis." In Annual European Congress of Rheumatology, 14–17 June, 2017. BMJ Publishing Group Ltd and European League Against Rheumatism, 2017. http://dx.doi.org/10.1136/annrheumdis-2017-eular.3894.

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Guice, Justin, Caroline Best, Morgan Hollins, Kelly Tinker, and Sean Garvey. "Fungal Digestive Enzymes Promote Macronutrient Hydrolysis in the INFOGEST in vitro Simulation of Digestion." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/agsn3911.

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Fungal enzymes are common ingredients in dietary supplements that support digestion. At adequate concentrations, exogenous enzymes may improve digestion by hydrolyzing macronutrients beyond acid-mediated hydrolysis, endogenous gastric pepsin, and pancreatic enzymes alone. The purpose of this study was to test the hydrolytic efficacy of 6 commercial fungal enzymes—three proteases, a lipase, an amylase, and a glucoamylase—in the INFOGEST static in vitro simulation of gastrointestinal digestion. The efficacy of 5 doses of each enzyme was determined by measuring free amino nitrogen (FAN), glycerol, maltose, and glucose concentrations following salivary-gastric (SG) and full salivary-gastric-intestinal (SGI) simulations of digestion of an oral nutritional supplement. In the SG simulation, the 3 proteases, lipase, and combination of amylase and glucoamylase promoted greater hydrolysis of dietary protein, fat, and carbohydrates, respectively, compared to control conditions. Acid protease (Aspergillus niger) treatment increased FAN concentrations than controls from 27% at the lowest dose to 142% greater than controls at the highest dose (p<0.0001). Fungal protease (A. oryzae) treatment increased FAN concentration at the highest dose (160,000 HUT, p=0.0115). Peptidase (A. melleus) treatment promoted higher FAN concentrations, up to 50% increase at the highest dose (160 LAPU, p<0.0001). Glycerol concentrations increased across all lipase treatments (p<0.0001), from 4.1-fold to 11.2-fold increases at the lowest and highest doses, respectively. All doses of amylase increased glucose concentrations (p<0.0001), and maltose concentrations started increasing at 4,000 SKB units (p=0.0010). In the SGI simulation, FAN concentrations following protease treatments were similar to control, suggesting little benefit beyond pancreatin alone in this static simulation of healthy digestion. Lipase and amylase/glucoamylase treatments, however, did increase glycerol (p<0.0001) and maltose/glucose concentrations (p<0.0001), respectively, compared to controls in the full SGI simulation. These data demonstrate that exogenous, fungal enzymes can improve macronutrient digestion under the acidic conditions of the gastric simulation, as well as the intestinal simulation.
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Zhengzhi, Wu, Wang Jiguo, Zhang Xiaoli, Cao Meiqun, and W. U. Anmin. "Preliminary studies on diagnostic cast of peptic ulcer based on saliva proteome and bioinformatics." In 2011 4th International Conference on Biomedical Engineering and Informatics (BMEI). IEEE, 2011. http://dx.doi.org/10.1109/bmei.2011.6098569.

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Svärd, Anna, Karin Roos Ljungberg, Mikael Brink, Alf Kastbom, and Solbritt Rantapää Dahlqvist. "SAT0067 SECRETORY ANTIBODIES TO CITRULLINATED PEPTIDES IN PLASMA AND SALIVA FROM RHEUMATOID ARTHRITIS PATIENTS AND THEIR UNAFFECTED FAMILY MEMBERS." In Annual European Congress of Rheumatology, EULAR 2019, Madrid, 12–15 June 2019. BMJ Publishing Group Ltd and European League Against Rheumatism, 2019. http://dx.doi.org/10.1136/annrheumdis-2019-eular.3477.

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