Добірка наукової літератури з теми "RoGFP2"
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Статті в журналах з теми "RoGFP2":
Albrecht, Simone C., Mirko C. Sobotta, Daniela Bausewein, Isabel Aller, Rüdiger Hell, Tobias P. Dick, and Andreas J. Meyer. "Redesign of Genetically Encoded Biosensors for Monitoring Mitochondrial Redox Status in a Broad Range of Model Eukaryotes." Journal of Biomolecular Screening 19, no. 3 (August 16, 2013): 379–86. http://dx.doi.org/10.1177/1087057113499634.
Liu, Ting-Hang, Mohammad A. Yaghmour, Miin-Huey Lee, Thomas M. Gradziel, Johan H. J. Leveau, and Richard M. Bostock. "An roGFP2-Based Bacterial Bioreporter for Redox Sensing of Plant Surfaces." Phytopathology® 110, no. 2 (February 2020): 297–308. http://dx.doi.org/10.1094/phyto-07-19-0237-r.
Xu, Xiuling, Katharina von Löhneysen, Katrin Soldau, Deborah Noack, Andrew Vu, and Jeffrey S. Friedman. "A novel approach for in vivo measurement of mouse red cell redox status." Blood 118, no. 13 (September 29, 2011): 3694–97. http://dx.doi.org/10.1182/blood-2011-03-342113.
Xu, Xiuling, Katharina von Loehneysen, Deborah Noack, Andrew Vu, and Jeff S. Friedman. "A Novel Approach for In Vivo Measurement of Red Cell Redox Status." Blood 116, no. 21 (November 19, 2010): 2036. http://dx.doi.org/10.1182/blood.v116.21.2036.2036.
de Cubas, Laura, Valeriy V. Pak, Vsevolod V. Belousov, José Ayté, and Elena Hidalgo. "The Mitochondria-to-Cytosol H2O2 Gradient Is Caused by Peroxiredoxin-Dependent Cytosolic Scavenging." Antioxidants 10, no. 5 (May 6, 2021): 731. http://dx.doi.org/10.3390/antiox10050731.
Xu, Xiuling, and Jeff S. Friedman. "In Vivo Monitoring of Red Cell Redox Status to Screen for Potential Hematotoxicity of Anti-Malarial Drugs." Blood 118, no. 21 (November 18, 2011): 2099. http://dx.doi.org/10.1182/blood.v118.21.2099.2099.
Fernández-Puente, Escarlata, and Jesús Palomero. "Genetically Encoded Biosensors to Monitor Intracellular Reactive Oxygen and Nitrogen Species and Glutathione Redox Potential in Skeletal Muscle Cells." International Journal of Molecular Sciences 22, no. 19 (October 8, 2021): 10876. http://dx.doi.org/10.3390/ijms221910876.
García-Quirós, Estefanía, Juan de Dios Alché, Barbara Karpinska, and Christine H. Foyer. "Glutathione redox state plays a key role in flower development and pollen vigour." Journal of Experimental Botany 71, no. 2 (September 26, 2019): 730–41. http://dx.doi.org/10.1093/jxb/erz376.
Morgan, Bruce, Mirko C. Sobotta, and Tobias P. Dick. "Measuring EGSH and H2O2 with roGFP2-based redox probes." Free Radical Biology and Medicine 51, no. 11 (December 2011): 1943–51. http://dx.doi.org/10.1016/j.freeradbiomed.2011.08.035.
Costa, Cláudio F., Celien Lismont, Serhii Chornyi, Hongli Li, Mohamed A. F. Hussein, Hans R. Waterham, and Marc Fransen. "Functional Analysis of GSTK1 in Peroxisomal Redox Homeostasis in HEK-293 Cells." Antioxidants 12, no. 6 (June 7, 2023): 1236. http://dx.doi.org/10.3390/antiox12061236.
Дисертації з теми "RoGFP2":
Dehaene, Noémie. "Functional analysis of a cytoplasmic male sterility in Arabidopsis thaliana." Thesis, Université Paris-Saclay (ComUE), 2017. http://www.theses.fr/2017SACLS418/document.
This work aims at better understand the events leading to pollen abortion in a recently discovered gametophytic cytoplasmic male sterility (CMS) in Arabidopsis thaliana. Although CMS have been widely used in hybrid seed production in many crops, the physiological mechanisms leading to pollen death by the mitochondrial sterilizing genes in the permissive (maintainer) nuclear backgrounds are poorly understood. Association genetics previously identified orf117Sha as a candidate mitochondrial CMS-associated gene.In a first part, I analyzed the expression of the orf117Sha gene in sterile plants and in fertile plants carrying nuclear genes restoring male fertility. I observed unusual features of its mRNA, but detected no difference at this level between sterile and restored plants. Oppositely, the ORF117SHA protein seems to be accumulated specifically in the sterile line, supporting its role in CMS. A phenocopy attempt by transgenesis suggested a possible link between a female and male gametophytic lethality and the ORF117SHA, even though few individuals could be analyzed.In a second part, I observed pollen development in sterile plants and fertile controls using different cytological approaches. My results show a progressive pollen death starting from the binucleate stage in the sterile. Prior to abortion, pollen mitochondria swell before rupture, and the development stops. I used confocal microscopy combined with genetically encoded sensors to explore specific physiological features in pollen and vegetative tissues of sterile plants. With ATeam, which allows the assessment of ATP content in the cytosol, I could challenge the generally accepted hypothesis of an ATP deficiency leading to pollen abortion in CMS. Indeed, the ATP production does not seem to be affected in the sterile line. With a mitochondria-addressed roGFP2-Grx1, I was able to assess the redox state of the glutathione pool in vegetative tissues and in the male gametophyte. I observed an overoxydation of the glutathione pool in mitochondria of the sterile line, in vegetative tissue investigated and in the pollen grain. This overoxydation seems to be linked to the CMS as it is annihilated by the presence of restorer genes.My results pave the way for further exploration of the links between the sterility protein, mitochondrial morphology changes, mitochondrial overoxydation, and pollen development arrest and death in the A. thaliana CMS
Caubrière, Damien. "Développement de nouveaux biosenseurs redox pour composés soufrés." Electronic Thesis or Diss., Université de Lorraine, 2022. http://www.theses.fr/2022LORR0359.
Over the last decade, the development of fluorescent redox biosensors has provided tools to study the in vivo dynamics of molecules such as the reduced and oxidized forms of glutathione or hydrogen peroxide. Cysteine being a key metabolite of sulfur metabolism, this PhD project aimed at developing a fluorescent redox biosensor specific for cysteine by coupling an oxidoreductase to roGFP2 (reduction-oxidation green fluorescent protein). First, the activities of several isoforms of cysteine desulfurases (CD) and rhodanese-domain containing proteins (Rhd), catalyzing cysteine desulfuration and trans-persufidation reactions, respectively, were analyzed in vitro in order to determine whether they could constitute good candidates for this oxidoreductase activity. These analyses revealed that a natural chimeric protein possessing both CD and Rhd domains efficiently oxidizes roGFP2, by catalyzing trans-persulfidation reactions from cysteine to roGFP2. This candidate protein was then fused to roGFP2 to generate the CD-Rhd-roGFP2 biosensor. In vitro, this protein is sensitive to oxidation in the presence of physiological concentrations of cysteine whereas oxidation by thiosulfate, another potential substrate of the Rhd domain, is negligible. In addition, the trans-persulfidation reactions between the protein domains leading to the oxidation of roGFP2 are not inhibited by physiological reducing systems. Nevertheless, the glutathione/glutaredoxin system specifically reduces roGFP2. The expression of this biosensor in the bacterium Escherichia coli revealed a dynamic response of the biosensor to exogenous addition of cysteine or cystine, paving the way for similar studies in organelles from other eukaryotic model organisms
Schneider, Jannis Frederic [Verfasser], Lars I. [Gutachter] Leichert, and Joachim [Gutachter] Rassow. "Untersuchung des Redoxzustandes des Periplasmas und Aufklärung früher redox-regulatorischer Ereignisse in Escherichia coli während der eukaryotischen Immunantwort unter Verwendung von roGFP2 basierten Sonden / Jannis Frederic Schneider ; Gutachter: Lars I. Leichert, Joachim Rassow ; Medizinische Fakultät." Bochum : Ruhr-Universität Bochum, 2021. http://d-nb.info/1235224279/34.
Maciejuk, Anna-Maria. "Measurements of redox potential during apoptosis." Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/28862.
Edwards, Sarah. "Disulfide-Mediated Modifications of roGFP and their Impact on Its Use as a Redox Sensor." Thesis, The University of Arizona, 2011. http://hdl.handle.net/10150/144316.
Wagener, Kerstin Charlotte [Verfasser], Michael [Akademischer Betreuer] Müller, Michael [Gutachter] Müller, and Stefan [Gutachter] Jakobs. "Transgene Redoxindikator-Mäuse mit mitochondrialer roGFP1-Expression: Phänotypisierung, neuronales Verteilungsmuster und Sensorfunktionalität / Kerstin Charlotte Wagener ; Gutachter: Michael Müller, Stefan Jakobs ; Betreuer: Michael Müller." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2017. http://d-nb.info/1149956364/34.
Souza, Arnaldo Henrique de. "Modulação redox, função e sobrevivência de células β-pancreáticas: evidência sobre o papel da enzima NADPH oxidase-2 (NOX2) em um modelo in vitro de glicotoxicidade." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/42/42137/tde-06092016-094234/.
Oxidative stress and NADPH oxidase-2 (NOX2) enzyme are associated to the decline of the functional β-cell mass in type 2 diabetes (T2D). Here, we tested the role of NOX2 on β-cell glucotoxicity. NOX2 knockout (NOX2 KO) and wild type (WT) C57BL/6J mice islets were isolated and cultured up to 3 weeks at 10 or 30 mmol/l glucose concentrations (G10 and G30, respectively). The insulin secretion was higher in NOX2-KO vs. WT islets despite similar metabolic and cytosolic glutathione-redox potential (EGSH) changes. The prolonged culture at G30 increases the H2O2 concentration and cytosolic thiol oxidation, followed by increased βcell apoptosis but preserving maximal secretory response. These responses were almost identical in both types of islets. In conclusion, NOX2 is a negative regulator of insulin secretion in C57BL/6J mouse islets, but is not a critical component for β-cell survival in a model of glucotoxicity in vitro.
Kolbrink, Benedikt [Verfasser], Michael [Akademischer Betreuer] Müller, and Jochen [Akademischer Betreuer] Staiger. "Charakterisierung eines transgenen Mausmodells mit spezifischer zytosolischer Expression des optischen Redox-Indikators roGFP1 in Neuronen / Benedikt Kolbrink. Gutachter: Michael Müller ; Jochen Staiger. Betreuer: Michael Müller." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2015. http://d-nb.info/1077096410/34.
Kizina, Kathrin Michaela. "Funktionelles ROS/Redox Imaging, basierend auf genetisch-kodierten optischen Sensoren, exzitationsratiometrischer Zwei-Photonen-Mikroskopie und Fluoreszenzlebenszeiten." Doctoral thesis, 2019. http://hdl.handle.net/21.11130/00-1735-0000-0003-C139-3.
Wagener, Kerstin Charlotte. "Transgene Redoxindikator-Mäuse mit mitochondrialer roGFP1-Expression: Phänotypisierung, neuronales Verteilungsmuster und Sensorfunktionalität." Doctoral thesis, 2017. http://hdl.handle.net/11858/00-1735-0000-0023-3F99-F.
Частини книг з теми "RoGFP2":
Ugalde, José Manuel, Lara Fecker, Markus Schwarzländer, Stefanie J. Müller-Schüssele, and Andreas J. Meyer. "Live Monitoring of ROS-Induced Cytosolic Redox Changes with roGFP2-Based Sensors in Plants." In Methods in Molecular Biology, 65–85. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2469-2_5.
Lismont, Celien, Paul A. Walton, and Marc Fransen. "Quantitative Monitoring of Subcellular Redox Dynamics in Living Mammalian Cells Using RoGFP2-Based Probes." In Methods in Molecular Biology, 151–64. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6937-1_14.
Buratti, Stefano, Matteo Grenzi, Giorgia Tortora, Sara Paola Nastasi, Elisa Dell’Aglio, Andrea Bassi, and Alex Costa. "Noninvasive In Planta Live Measurements of H2O2 and Glutathione Redox Potential with Fluorescent roGFPs-Based Sensors." In ROS Signaling in Plants, 45–64. New York, NY: Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3826-2_4.
Тези доповідей конференцій з теми "RoGFP2":
Niculescu, Mihai Alexandru, Stefan Ruseti, and Mihai Dascalu. "RoGPT2: Romanian GPT2 for Text Generation." In 2021 IEEE 33rd International Conference on Tools with Artificial Intelligence (ICTAI). IEEE, 2021. http://dx.doi.org/10.1109/ictai52525.2021.00183.
Wierer, S., K. Elgass, S. Bieker, U. Zentgraf, A. J. Meixner, and F. Schleifenbaum. "Determination of the in vivo redox potential using roGFP and fluorescence spectra obtained from one-wavelength excitation." In SPIE BiOS, edited by Daniel L. Farkas, Dan V. Nicolau, and Robert C. Leif. SPIE, 2011. http://dx.doi.org/10.1117/12.873753.
Звіти організацій з теми "RoGFP2":
Friedman, Haya, Chris Watkins, Susan Lurie, and Susheng Gan. Dark-induced Reactive Oxygen Species Accumulation and Inhibition by Gibberellins: Towards Inhibition of Postharvest Senescence. United States Department of Agriculture, December 2009. http://dx.doi.org/10.32747/2009.7613883.bard.