Дисертації з теми "RNA Syntheis"
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Fritz, Sarah E. "Molecular basis of the DExH-box RNA helicase RNA helicase A (RHA/DHX9) in eukaryotic protein synthesis." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1437413252.
Повний текст джерелаJohnston, Julie Catherine. "In vitro translation of cucumber necrosis virus RNA." Thesis, University of British Columbia, 1989. http://hdl.handle.net/2429/28969.
Повний текст джерелаLand and Food Systems, Faculty of
Graduate
Peters, D. W. "RNA synthesis in Candida albicans." Thesis, University of Warwick, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373051.
Повний текст джерелаLackey, Jeremy. "New methods for the synthesis of RNA, novel RNA pro-drugs and RNA microarrays." Thesis, McGill University, 2010. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=92290.
Повний текст джерелаA major goal of this thesis work was aimed at finding ribonucleoside synthons that potentially benefit two critical aspects of RNA manufacturing: yield and ease of post-synthesis processing. Towards these goals, we developed methods for the synthesis of RNA using 2'-O-Lv and 2'-O-acetal Lv (ALE) ribonucleoside derivatives. Deprotection of the RNA chains consisted of a three-step deprotection scheme, which eliminated the need for any harsh basic hydrolytic steps, generally composed of: (1) treatment with anhydrous NEt3 (r.t., 1 h) to deblock the phosphate's cyanoethyl groups; (2) hydrazinolysis (r.t., 30 min 4 h) to simultaneously deprotect the nucleobases and 2'-OH positions, and (3) fluoride treatment (r.t., 30 min) to effect cleavage from the controlled pore glass solid support. Significantly, the rather mild conditions to remove 2'-O-Lv or 2'-O-ALE protecting groups did not lead to RNA strand scission. Furthermore, in the case of 2'-O-ALE protection, higher step-wise monomer coupling yields (~98.7%) was possible, since the ALE protection is less bulky than conventional silyl protection, i.e. TBDMS. Furthermore, both 2'-O-Lv or 2'O-ALE chemistries are completely compatible with the synthesis cycles used by all automated gene synthesizers.
With adjustments in protecting group strategies for the 5'-OH, exocyclic amino nucleobase groups and the development of a light-labile solid support, two other major goals were achieved: (1) the first in situ synthesis of RNA on microarrays, and (2) synthesis of chemically modified RNA strands with 2'-O-acetal ester and 2'-O-acetal ester pyrrolidines in order to increase lipophilicity and cellular permeability over native RNA. When RNA synthesis was carried out with 5'-O-NPPOC 2'-O-ALE monomers on a microarray ("chip"), deprotection typically involved (1) cleavage of the photolabile 5'-protecting group; (2) treatment with anhydrous NEt3 (r.t., 1 h) to deblock the phosphate's cyanoethyl groups; (3) hydrazinolysis (r.t., 30 min 4 h) to simultaneously deprotect bases and 2'-OH positions. The latter step could also be accomplished with ethylenediamine at room temperature. An RNase A assay was performed as "proof-of-principle" to demonstrate the value of a DNA-RNA microarray for studying enzyme kinetics and specificity on oligonucleotide based libraries. We showed that RNase A acts effectively on a DNA-RNA substrate with measurable kinetics analogous to those of the reference substrates.
The novel 2'-O-modified RNA were tested as short interfering RNA pro-drugs ("pro-siRNA") that would cross the cell membrane and be hydrolyzed (at the 2'-O-ester groups) by ubiquitous esterases to release the active (siRNA) molecules. Indeed, both siRNA and pro-siRNA prepared via 2'-O-ALE chemistry were shown to be active in an RNAi luciferase gene knockdown assay, confirming the integrity of the synthesized RNA strands and the promise of the pro-siRNA approach.
Brown, Michael Dean. "Genetic analysis of RNA splicing in the thymidylate synthase gene of bacteriophage T4." Diss., Georgia Institute of Technology, 1990. http://hdl.handle.net/1853/25390.
Повний текст джерелаAttwater, James. "Ice as a medium for RNA-catalysed RNA synthesis and evolution." Thesis, University of Cambridge, 2011. https://www.repository.cam.ac.uk/handle/1810/246525.
Повний текст джерелаCollis, Alana E. C. "The synthesis of vinylphosphonate-linked RNA." Thesis, University of Nottingham, 2008. http://eprints.nottingham.ac.uk/10541/.
Повний текст джерелаLiu, Qi. "Synthesis of small molecules targeting RNA /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2004. http://wwwlib.umi.com/cr/ucsd/fullcit?p3142456.
Повний текст джерелаFinnegan, Patrick Michael. "RNA synthesis in maize mitochondria : the identification of autonomously replicating RNA species and a kinetic analysis of transcript accumulation." Thesis, McGill University, 1989. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=75931.
Повний текст джерелаAlthough initiation and processing probably occur at reduced levels in isolated maize mitochondria, endogenous DNA templates are extensively transcribed at the same relative rates as in vivo. Isolated maize mitochondria were used to demonstrate that differential rates of both synthesis and turnover help determine the steady-state abundances of various mitochondrial RNA sequences and that mitochondria from certain lines possess an autonomously-replicating, RNA-based genetic system.
D'Abramo, Claudia M. "Biochemical characterization of the BVDV RNA-dependent RNA polymerase during initiation and elongation of RNA synthesis." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=111870.
Повний текст джерелаMatsuura, Satoshi. "Synthetic RNA-based logic computation in mammalian cells." Kyoto University, 2019. http://hdl.handle.net/2433/242426.
Повний текст джерелаRepass, John F. "Studies of murine coronavirus cis-acting RNA elements that affect RNA synthesis /." Digital version accessible at:, 2000. http://wwwlib.umi.com/cr/utexas/main.
Повний текст джерелаWebb, Vera Ann B. "In vivo in vitro synthesis of ribosomal RNA in bacillus subtilis." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/29448.
Повний текст джерелаScience, Faculty of
Microbiology and Immunology, Department of
Graduate
Wang, Yang. "Chemical synthesis of DNA and RNA containing thio-substituted bases and their post-synthetic modifications." Thesis, Aston University, 2003. http://publications.aston.ac.uk/11013/.
Повний текст джерелаUsman, Nassim. "Nucleoside phosphoramidites in the automated, solid phase synthesis of oligoribonucleotides and their analogues : the chemical synthesis of an E. Coli N-Formyl-Methionine tRNA." Thesis, McGill University, 1986. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=75344.
Повний текст джерелаA reaction occurring at the O$ sp6$-position of guanosine residues, which leads to chain cleavage, was identified. The basis of this reaction was characterized by both $ sp{31}$P nuclear magnetic resonance and the synthesis of oligoriboguanylate sequences. Three methods involving: protection of the O$ sp6$-position, the use of an alternate coupling cycle, and the use of a different phosphoramidite activator, were successfully applied to the resolution of this problem. The aforementioned procedures were utilized in the synthesis of a half E. Coli N- f - met tRNA molecule 43 units in length.
Finally the cumulative knowledge gained from the above studies was applied to the synthesis of an entire E. Coli N- f - met analogue 77 units in length along with a number of other very long sequences. Methods for the deprotection of these oligomers were investigated culminating in the isolation of oligoribonucleotides which were successfully characterized by polyacrylamide gel electrophoresis, enzyme degradation, enzymatic and chemical sequencing, terminal nucleotide analysis, and, in the case of the E. Coli f - met tRNA analogue, an aminoacylation assay.
Hamilton, Andrew John. "Inhibiting gene expression with anti-sense RNA." Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.316938.
Повний текст джерелаAkhtar, Y. "Studies on the maturation pathway of ribosomal precursor RNA : Analysis of Xenopus ribosomal RNA synthesised by transcription in vitro." Thesis, University of Liverpool, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.382054.
Повний текст джерелаGilea, Manuela Aurora. "DNA and RNA synthesis in ionic liquids." Thesis, Queen's University Belfast, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.485198.
Повний текст джерелаSchulz, Daniel. "Transcriptome surveillance in S. cerevisiae by RNA synthesis and degradation coupling and selective termination of non-coding RNAs." Diss., Ludwig-Maximilians-Universität München, 2013. http://nbn-resolving.de/urn:nbn:de:bvb:19-163595.
Повний текст джерелаGarcia, Martin Juan Antonio. "RNA inverse folding and synthetic design." Thesis, Boston College, 2016. http://hdl.handle.net/2345/bc-ir:106989.
Повний текст джерелаThesis advisor: Peter G. Clote
Synthetic biology currently is a rapidly emerging discipline, where innovative and interdisciplinary work has led to promising results. Synthetic design of RNA requires novel methods to study and analyze known functional molecules, as well as to generate design candidates that have a high likelihood of being functional. This thesis is primarily focused on the development of novel algorithms for the design of synthetic RNAs. Previous strategies, such as RNAinverse, NUPACK-DESIGN, etc. use heuristic methods, such as adaptive walk, ensemble defect optimization (a form of simulated annealing), genetic algorithms, etc. to generate sequences that minimize specific measures (probability of the target structure, ensemble defect). In contrast, our approach is to generate a large number of sequences whose minimum free energy structure is identical to the target design structure, and subsequently filter with respect to different criteria in order to select the most promising candidates for biochemical validation. In addition, our software must be made accessible and user-friendly, thus allowing researchers from different backgrounds to use our software in their work. Therefore, the work presented in this thesis concerns three areas: Create a potent, versatile and user friendly RNA inverse folding algorithm suitable for the specific requirements of each project, implement tools to analyze the properties that differentiate known functional RNA structures, and use these methods for synthetic design of de-novo functional RNA molecules
Thesis (PhD) — Boston College, 2016
Submitted to: Boston College. Graduate School of Arts and Sciences
Discipline: Biology
Wesselhoeft, R. Alexander(Robert Alexander). "Synthetic circular RNA for protein expression." Thesis, Massachusetts Institute of Technology, 2019. https://hdl.handle.net/1721.1/122710.
Повний текст джерелаCataloged from PDF version of thesis.
Includes bibliographical references (pages 111-126).
Messenger RNA (mRNA) has broad potential for therapeutic and engineering applications. One fundamental limitation of mRNA is its relatively short half-life in biological systems, effected in part by rapid exonuclease-mediated degradation upon delivery. Circular RNA (circRNA), a type of single-stranded RNA with a contiguous structure that lacks the end motifs necessary for exonuclease recognition, may be resistant to this mechanism of degradation and therefore may exhibit superior stability. However, challenges in circularization, purification, and protein expression have impeded a thorough investigation of exogenous circRNA. By rationally designing ubiquitous accessory sequences to facilitate circularization, we engineered a permuted self-splicing intron that efficiently circularized RNAs up to 5kb in length in vitro.
With the addition of these accessory sequences, we were able to demonstrate nearly complete circularization of precursor RNAs containing an internal ribosome entry site (IRES) for translation initiation and a coding region such as erythropoietin or eGFP. We found that translation from optimized circRNA was robust, and circRNA protein expression stability far exceeded that of both unmodified and nucleoside modified linear mRNA in some cellular contexts. We monitored cytokine release and antiviral defense induction in sensitive cells transfected with circRNA purified by different methods and found that the immunogenicity and stability of circRNA preparations was dependent on the degree of purity, with small amounts of contaminating linear RNA leading to robust cellular immune responses.
In contrast to purified unmodified linear mRNA, purified unmodified circRNA was invisible to several RNA sensors including RIG-i and endosomai toil-like receptors (TLRs) and did not provoke a significant cytokine response upon transfection. Using purified circRNA, we finally provided the first demonstration to our knowledge of exogenous circRNA delivery and translation in vivo, and showed that the duration of circRNA translation was extended in adipose tissue in comparison to unmodified and uridine-modified linear mRNAs. In total, this work suggests that circRNA is a promising alternative to linear mRNA for therapeutic applications.
by R. Alexander Wesselhoeft.
Ph. D.
Ph.D. Massachusetts Institute of Technology, Department of Biology
Wang, Muhan. "Studies of piRNA synthesis." Thesis, University of Oxford, 2011. http://ora.ox.ac.uk/objects/uuid:1e134b7b-4e9d-4d0e-bfd8-e4696f960ad6.
Повний текст джерелаJohnson, Moira A. "Kinetics of RNA synthesis in Rotavirus infected cells." Thesis, University of Warwick, 1988. http://wrap.warwick.ac.uk/98142/.
Повний текст джерелаWhitfield, Julie Nicole. "Studies on a potentially prebiotic synthesis of RNA." Thesis, University of Oxford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320683.
Повний текст джерелаCook, Stephen D. "Studies on a potentially prebiotic synthesis of RNA." Thesis, University of Oxford, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.267951.
Повний текст джерелаTakeuchi, Akiko. "RNA-protein interaction in the selenoprotein synthesis machinery." Strasbourg, 2009. http://www.theses.fr/2009STRA6054.
Повний текст джерелаThe 21st amino acid selenocysteine is encoded by a UGA codon that usually signifies translational termination. Selenoprotein synthesis therefore requires specialized factors. Among these is SBP2 that binds the SECIS, a stem-loop structure in the 3’UTR of selenoprotein mRNAs. In structural analyses of SBP2, we isolated and functionally characterized Drosophila melanogaster SBP2. By comparing it with human SBP2, we identified an additional RNA binding domain that is essential for SECIS and 60S ribosomal subunit binding, and also enables SECIS structure selectivity. In addition, computational and biophysical analyses established that SBP2 is globally unfolded, supporting our hypothesis that SBP2 is an Intrinsically Disordered Protein and becomes folded in the presence of partners yet to be identified. Finally, we searched for potential partners of SBP2 and our results showed that the molecular assembly of selenoprotein mRNPs has many similarities with that of sn/snoRNPs
Chen, Yuanyuan. "Characterizing RNA Structure and synthesis by Raman Microscopy." Case Western Reserve University School of Graduate Studies / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=case1277761094.
Повний текст джерелаYunus, Muhammad Amir. "Studies on the mechanism of norovirus RNA synthesis." Thesis, Imperial College London, 2012. http://hdl.handle.net/10044/1/9811.
Повний текст джерелаWang, Hai. "Design, synthesis and RNA binding of aminoglycoside antibiotics /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 1998. http://wwwlib.umi.com/cr/ucsd/fullcit?p9820848.
Повний текст джерелаTakeuchi, Akiko Krol Alain Allmang-Cura Christine. "RNA-protein interaction in the selenoprotein synthesis machinery." Strasbourg : Université de Strasbourg, 2009. http://eprints-scd-ulp.u-strasbg.fr:8080/1133/01/TAKEUCHI_Akiko_2009.pdf.
Повний текст джерелаThèse soutenue sur un ensemble de travaux. Titre provenant de l'écran-titre. Bibliogr. 11 p.
York, Ashley D. "A study of viral and cellular factors in the regulation of the influenza virus RNA-dependent RNA polymerase." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:5958fafd-4c91-4434-910e-29e2dd0539b9.
Повний текст джерелаJia, Yiping. "Mechanistic studies of DNA-dependent transcription initiation and RNA synthesis by bacteriophage T7 RNA polymerase /." The Ohio State University, 1998. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487953204281995.
Повний текст джерелаBhutia, Pema choden. "Accuracy of TransferRNA Selection in Protein synthesis." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-162811.
Повний текст джерелаFormanová, Nataša. "A complex synthesizing the maize mitochondrial plasmid RNA b /." Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=68173.
Повний текст джерелаLee, Hung-chiu. "Synthetic RNA interference against influenza A virus." Click to view the E-thesis via HKUTO, 2005. http://sunzi.lib.hku.hk/hkuto/record/B35537814.
Повний текст джерелаLee, Hung-chiu, and 李洪釗. "Synthetic RNA interference against influenza A virus." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2005. http://hub.hku.hk/bib/B35537814.
Повний текст джерелаChe, Austin 1979. "Engineering RNA logic with synthetic splicing ribozymes." Thesis, Massachusetts Institute of Technology, 2008. http://hdl.handle.net/1721.1/47786.
Повний текст джерелаIncludes bibliographical references (p. 169-185).
Reusable components, such as logic gates and code libraries, simplify the design and implementation of electronic circuits and computer programs. The engineering of biological systems would benefit also from reusable components. In this thesis, I show the utility of splicing ribozymes for the biological engineer. Ribozymes allow the engineer to manipulate existing biological systems and to program self-modifying RNA systems. In addition, splicing ribozymes are easy to engineer, malleable, modular, and scalable. I used the model ribozyme from Tetrahymena to explore the principles behind engineering biological splicing systems in vivo. I show that the core ribozyme is modular and functions properly in many different contexts. Simple base pairing rules and computational RNA folding can predict splicing efficiency in bacterial cells. To test our understanding of the ribozyme, I generated synthetic ribozymes by manipulating the primary sequence while maintaining the secondary structure. Results indicate that our biochemical understanding of the ribozyme is accurate enough to support engineering. Splicing ribozymes can form core components in an all-RNA logic system. I developed biological transzystors, switches analogous to electrical transistors. Transzystors can use any trans-RNA as input and any RNA as output, allowing the genetic reading of RNA levels. I also show the ribozyme can write RNA using the trans-splicing reaction.
(cont.) Trans-splicing provides an easy mechanism to hook into an existing biological system and patch its operation. The generality of these ribozymes for a wide set of applications makes them promising tools for synthetic biology. Keywords: synthetic biology, RNA, Tetrahymena, ribozyme, splicing, transzystor.
by Austin J. Che.
Ph.D.
Thomas, Gregory Stuart. "Targeting prostate cancer with synthetic RNA ligands." Diss., University of Iowa, 2012. https://ir.uiowa.edu/etd/1508.
Повний текст джерелаYu, Hongchuan. "Novel Cyclo Deoxynucleoside: Synthesis and Evaluation." Thesis, Boston College, 2012. http://hdl.handle.net/2345/2751.
Повний текст джерелаThesis advisor: Mary F. Roberts
Nucleic acids are essential biological molecules for life. For example, deoxyribonucleic acid (DNA) is the main genetic information carrier; ribonucleic acid (RNA) plays a critical role in translation and transcription. These characteristics place nucleic acids as the fundamental genetic materials of a living system. Since over a century ago, intensive attempts have been made by researchers to study the nucleic acid properties. For chemists, it is particularly interesting and important to understand the relationship between structures and properties of nucleic acids. For instance chemical modifications can alter stability of nucleic acids, and consequently influence their biochemical behaviors. In this work, we began by investigation of a 5',6-cyclo-modified nucleic acid resembling the product of DNA oxidation, and then developed a library of cyclomodifications. Our research on their structures and properties indicated that by installing cyclo-modifications we might be able to add some properties, that were not observed in nature to nucleic acids
Thesis (PhD) — Boston College, 2012
Submitted to: Boston College. Graduate School of Arts and Sciences
Discipline: Chemistry
Steger, Courtney Long. "Determinants of Core Shell Dependent Rotavirus Polymerase Activity." Diss., Virginia Tech, 2019. http://hdl.handle.net/10919/87755.
Повний текст джерелаPh. D.
Rotaviruses (RVs) are clinically-significant gastrointestinal pathogens that cause severe diarrhea and dehydration in children. RVs encode a specialized polymerase enzyme, called VP1, which functions to synthesize RNA during viral replication. RNA synthesis activities of VP1 are tightly regulated by another RV protein, VP2, which comprises the innermost core shell layer of the virion. Though these VP1-VP2 interactions are essential for viral replication, the mechanism by which the core shell supports polymerase activity remains poorly understood. Here, we sought to identify and characterize specific regions of both VP1 and VP2 that are essential for polymerase activity in a test tube (i.e., in vitro). First, we analyzed VP1 and VP2 sequence diversity across many RV strains. Then, we tested how the identified sequence differences influenced VP2-dependent VP1 activation in vitro. To pinpoint critical regions of VP1 and VP2, we next engineered and assayed several mutant proteins. Altogether, our results revealed several functionally important residues of VP1 and VP2, which raises new ideas about VP1-VP2 binding interface(s) that are important for viral replication. Moreover, results from these studies may provide a scientific platform for the rational design of next-generation RV vaccines or antiviral therapeutics.
Hannoush, Rami Nabil. "RNA folding : synthesis, structure and biological properties of hairpins based on 2',5'-linked RNA loops." Thesis, McGill University, 2002. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=82890.
Повний текст джерелаOne aspect of this thesis deals with the synthesis and structural studies of 2',5'-dodecaribonucleotides. Their base sequence was designed to promote intramolecular folding by base pairing leading to the formation of "hairpin loops". The hairpins consist of a tetranucleotide loop and a 4 base-pair duplex (stem). The thermodynamic stability and conformation of these hairpins were assessed by UV, CD and NMR spectroscopy. Melting experiments (UV) revealed that with a few exceptions, hairpins comprised of 2',5'-(UUCG) loops exhibit greater thermodynamic stability (e.g. Tm) than the corresponding hairpins with 3' ,5'-linked loops. The 'extra' stability imparted by the 2',5'-UUCG) loop was found to be virtually independent of the sugar composition of the stem. For example, the 2',5'-tetraloop stabilizes hairpins whether their duplex stem is based on double-stranded DNA or RNA. In contrast, the 3',5-tetraloop stabilizes hairpins only when their stem duplex is double-stranded RNA. NMR studies revealed that the 2',5'-tetraloop (UUCG) is self-stabilized by a wobble U·G base pair, extensive base stacking and sugar-base contacts. A more striking feature is the protrusion of the cytosine residue into the solvent without participating in any of the loop stabilizing interactions. This identifies the 2',5 '-linked (UUCG) loop as a novel structural motif and provides the first demonstration that 2',5' can fold back on itself to form a hairpin structure of unusual thermodynamic stability.
The ability of hairpin structures to inhibit human immunodeficiency virus (HIV-1) reverse transcriptase (RT) was also evaluated. Four potent hairpins based on 3',5'- and 2' ,5'-RNA were able to inhibit the RNase H activity of HIV-1 reverse transcriptase, a key enzyme for the proliferation of the human immunodeficiency virus (HIV-1). The polymerase activity of HIV-1 RT was not affected by this class of oligonucleotides. The hairpins were identified from a nucleic acid library synthesized via diversity-oriented solid-phase synthesis. These compounds represent the first examples of hairpins, 12 nucleotides in length, that inhibit specifically the RNase H activity of HIV-1 RT without affecting its polymerase activity.
Another study in this work dealt with yeast RNase III (Rnt1p), an enzyme implicated in the mechanism of action of RNA interference (RNAi). Through these investigations, it was discovered that Rntlp cleaves the DNA strand of DNA:RNA hybrids. This was totally unexpected since Rntlp, like other RNase III enzymes, was thought to be specific only towards the cleavage of double-stranded RNA. These studies also increased our knowledge about the mechanism by which the enzyme cleaves the target RNA (or DNA) strand and suggest that the vicinal 2'-hydroxyl group on the ribose sugar does not participate directly in the cleavage reaction.
Finally, the formation of C-tetrad structures (i-motif) was induced through the design and synthesis of extra-stable hairpin loops containing deoxycytidine rich stems. The corresponding hairpins containing ribocytidine folded into duplexes rather than C-tetrad structures. These studies lead to the first detection and identification of antiparallel 2',5 '-RNA duplexes based on hemiprotonated C·C+ base pairs.
Tetzlaff, Charles N. "Synthesis and evaluation of acylated DNA and RNA oligomers /." Thesis, Connect to Dissertations & Theses @ Tufts University, 2001.
Знайти повний текст джерелаAdviser: Clemens Richert. Submitted to the Dept. of Chemistry. Includes bibliographical references (leaves 228-235). Access restricted to members of the Tufts University community. Also available via the World Wide Web;
Roberts, Lisa Oriel. "Internal initiation of protein synthesis on picorna virus RNA." Thesis, University of Kent, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.245654.
Повний текст джерелаZhang, Ze. "The control of ribosomal RNA synthesis in mammalian cells." Thesis, University of Southampton, 2013. https://eprints.soton.ac.uk/350477/.
Повний текст джерелаDingwall, C. "The accumulation of proteins in the Xenopus oocyte nucleus." Thesis, Open University, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.354967.
Повний текст джерелаGreenhalgh, Duncan Alan. "Effects of 5-fluorouracil on RNA metabolism in human tumour cells." Thesis, University of Leeds, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.236315.
Повний текст джерелаPertile, Jack James. "Synthesis of Fluorescent Promazines and Evaluation of Photophysical Properties." OpenSIUC, 2019. https://opensiuc.lib.siu.edu/theses/2524.
Повний текст джерелаLi, Tin-wai Olive, and 李天慧. "Influenza polymerase subunit compatibility between human H1 and H5 viruses." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B41896890.
Повний текст джерелаMartin, Alarcon Daniel Alberto. "Tools for RNA and cell-free synthetic biology." Thesis, Massachusetts Institute of Technology, 2016. http://hdl.handle.net/1721.1/104124.
Повний текст джерелаThis electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.
Cataloged from student-submitted PDF version of thesis.
Includes bibliographical references (pages 58-63).
Amid the myriad recent developments in synthetic biology, progress has been fastest in the areas with the most versatile tools for understanding and engineering biological systems. RNA synthetic biology and synthetic minimal cells are areas where design is limited by the availability of tools to observe, program, and manipulate the systems in question. In this work I present expanded toolsets to achieve these goals. The ability to monitor and perturb RNAs in living cells would benefit greatly from a modular, programmable protein architecture for targeting unmodified RNA sequences. I report that the RNA-binding protein PumHD (Pumilio homology domain), which has been widely used in native and modified form for targeting RNA, can be engineered to yield a set of four canonical protein modules, each of which targets one RNA base. These modules (which I call Pumby, for Pumilio-based assembly) can be concatenated in chains of varying composition and length, to bind desired target RNAs. I validate that the Pumby architecture can perform RNA-directed protein assembly and enhancement of translation of RNAs. I further demonstrate a new use of such RNA-binding proteins, measurement of RNA translation in living cells. Pumby may prove useful for many applications in the measurement, manipulation, and biotechnological utilization of unmodified RNAs in intact cells and systems. Genetic circuits are a fundamental tool in synthetic biology; an open question is how to maximize the modularity of their design, to facilitate their integrity, scalability, and flexibility. Liposome encapsulation enables chemical reactions to proceed in well-isolated environments. I here adapt liposome encapsulation to enable the modular, controlled compartmentalization of genetic circuits and cascades. I demonstrate that it is possible to engineer genetic circuit-containing synthetic minimal cells (synells) so that they contain multiple-part genetic cascades, that these cascades can be controlled by external as well as inter-liposomal communication without cross-talk, and that these cascades can also be fused in a controlled way so that the products of incompatible reactions can be brought together. Synells thus enable more modular creation of synthetic biology cascades, an essential step towards their ultimate programmability.
by Daniel Alberto Martin Alarcon.
Ph. D.
Smith, Richard Wilson. "RNA metabolism and the control of protein synthesis in fish." Thesis, University of Aberdeen, 1996. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU089891.
Повний текст джерела