Дисертації з теми "Rhbmp2"
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Valenzuela, Juan Carlos Bustos. "Análise dos genes diferencialmente expressos durante a osteodiferenciação induzida por proteínas morfogenéticas de osso (BMP2 e BMP7) em células C2C12 e super-expressão de rhBMP2 e rhBMP7 em células de mamíferos." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-31072009-140027/.
Повний текст джерелаThe BMPs (Bone Morphogenetic Proteins) are members of the TGF-β (Transforming Growth Factor β) superfamily of proteins, regulate growth and differentiation of various cell types in various tissues, and some play a critical role in differentiation of mesenchymal cells into osteoblasts. Particularly, rhBMP2 and rhBMP7, promote osteoinduction \"in vitro\" and \"in vivo\" and both proteins are used therapeutically in Orthopedics and Dentistry. The differential expression of genes during osteodifferentiation induced by rhBMP2 and rhBMP7 in C2C12 cells was analyzed through DNA microarrays, allowing the selection of 31 genes, of which 24 were validated by qPCR, 13 of which are related to transcription, four associated with cell signaling pathways and seven are associated with the extracellular matrix. Subsequent functional analysis of these genes should reveal more details on the molecular events which take place during C2C12 cells osteoblastic differentiation induced by rhBMPs In paralel, rhBMPs 2 and 7 were overexpressed in HEK293T cells and BMP7 activity to induce osteodifferentiation \"in vitro\" and bone formation \"in vivo\" was demonstrated, reinforcing the viability of our objective to produce these proteins for future application as biopharmaceuticals in Brazil.
LECOEUR, ALAIN DIT LOIC. "Etude de l'induction de la differenciation osteoblastique sur les cellules stroma-vasculaires de tissu adipeux extramedullaire en culture sous l'influence de la proteine recombinante humaine rhbmp2 et de la dexamethasone : chez le lapin et chez l'humain." Paris 7, 1997. http://www.theses.fr/1997PA07GA06.
Повний текст джерелаGonzaga, Miliane Gonçalves. "Estudo qualitativo e quantitativo da interação entre a proteína morfogenética rhBMP-2 e diferentes tipos de enxertos ósseos." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/17/17142/tde-29052015-110800/.
Повний текст джерелаProteins with osteoinductive potential, particularly the rhBMP-2, have great effects on growth and cell differentiation. This study aimed to evaluate the relationship between different bone grafts and rhBMP-2 on the repair of critical bone defects in rat skulls. Fifty six Wistar rats, male, approximately 250g of body weight were used, sacrificed six weeks after surgical experiment, and divided into 8 groups(n=7): autograft (AUT); homograft (HOM); xenograft (HET); autograft and 5 g rhBMP-2 (AUT BMP); homograft and 5 g rhBMP-2 (HOM BMP); xenograft and 5 g rhBMP-2 (HET BMP); 5 g rhBMP-2 (BMP) and critical bone defect only (DOC). The results were analyzed statistically by ANOVA and Tukey post-test with multiple comparisons (p<0.05 of significance level), after the use of qualitative methodologies, micro CT morphology and histological analysis. According to the quantification of newly formed bone volume, all groups had better results than DOC group with a significant difference. The HOM and HET groups showed significant difference than AUT BMP, HOM BMP and HET BMP, that had higher bone formation, while AUT group was significantly different from HOM BMP and HET BMP. The BMP group had no significant difference in relation to AUT, HOM, HET, AUT BMP, HOM BMP and HET BMP. The AUT, HOM and HET groups had no significant difference between them, and also the groups AUT BMP, HOM BMP and HET BMP between them. Regarding the quantification of osteoclasts, there was no significant difference between groups; however, HET BMP had more osteoblastic cells than DOC group, with significant difference. For osteocytes quantification, AUT and DOC showed few cells. Thus, DOC had significant difference in relation to BMP, HET, HOM BMP and HET BMP; and AUT had significant difference in relation to BMP, HET, HOM BMP and HET BMP. Likewise, AUT BMP had significant difference compared to HET group, which showed more cells. Regarding to collagen fibers quantification, there was no significant difference between groups. New bone formation progressed from the edges to the center of the defect and for BMP group the newly formed bone occupied more than two thirds of the defect. DOC group showed a narrow band of newly formed bone at the edges and the remainder of the defect was filled by connective tissue. For micro CT analysis, all groups showed greater bone formation than the DOC group, being greater in AUT group than HOM and HET. Furthermore, the various grafts associated with rhBMP-2 showed greater bone formation than the isolated grafts, highlighting the AUT BMP. Therefore, it was concluded that rhBMP-2 helped bone repair when administered alone or associated with different bone grafts, giving better results when combined to the grafts
Freitas, Maria da Conceição Andrade de. "Análise histológica da região alveolar enxertada com rhBMP-2 em pacientes com fissuras labiopalatinas." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/61/61132/tde-06062017-085349/.
Повний текст джерелаObjective: This study aimed to evaluate histologically the bone and soft tissue removed from the region of the rhBMP-2-grafted alveolar cleft in patients with cleft lip and palate 6 to 46 months after surgery. Material and Methods: The experimental group consisted of 13 patients with canines retained in the alveolar region grafted with rhBMP-2 (Infuse®). The control group consisted of six patients with canines retained in the contralateral alveolar region or near the ungrafted cleft and one with canine retained in the grafted area with autogenous iliac crest bone. At the time of surgical exposure of the canine, 6 to 46 months after secondary bone graft, biopsies of bone and soft tissues were submitted to histological analysis by the hematoxylin and eosin technique. Results: The microscopic sections (n = 12) showed fragments of viable bone tissue with normal osteocytes in gaps, osteoblasts in the bone surface and spinal spaces filled by fibrous connective tissue and blood vessels. The bone tissue biopsies removed at a 6 to 9 months after grafting showed more disorganized bone trabeculae, with no lamellar formations and with a higher osteocyte density per area indicating a lower degree of bone maturity. Biopsies taken from 24 to 46 months after grafting were composed only for mature lamellar bone tissue containing reversion / incremental lines and osteocyte gaps. Soft tissue sections (n= 3) revealed fragments of buccal mucosa consisting of parakeratinized stratified squamous epithelium with fibrous connective tissue. Conclusion. Fragments of bone and soft tissues removed from the rhBMP-2 grafted cleft area showed normal histological morphology, resembling tissue characteristics on the control group.
Leal, Claudia Resende. "Enxerto alveolar com proteína morfogenética óssea (rhBMP-2) na fissura labiopalatina: influência da idade, do cirurgião, do tipo e da amplitude da fissura." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/61/61132/tde-17102016-142925/.
Повний текст джерелаObjective: To evaluate whether the canine irruption stage at the time of surgery, the type and width of cleft lip and palate and the surgeon influence on the outcome of alveolar graft surgeries performed with bone morphogenetic protein (rhBMP-2). Material and methods: This cross-sectional study evaluated a sample 90 individuals who consecutively underwent alveolar graft surgery with rhBMP-2 in a single center. Periapical radiographs were taken before and 6 months after surgery. Four experienced surgeons operated all patients. The result of alveolar graft was characterized in success or failure by three raters based on the criteria adopted by both the modified Bergland and Chelsea scales. The canine irruption stage at the time of the procedure, the type and width of cleft lip and palate and the surgeon who performed the surgery were considered. The larger width of the alveolar cleft was measured on periapical radiographs taken preoperatively using the software VISTA perio-plus scan (Dürr Dental, Bietigheim-Bissingen, Germany). Intra and inter-rater agreements were evaluated using Kappa coefficient. The intra-rater agreement for the variable cleft width was evaluated using Dahlberg test, paired t-tests and the Intraclass Correlation Coefficient (ICC). The influence of the independent variables in the outcome of alveolar graft was analyzed using Multivariate Logistic Regression (p<0.05). Results: All independent variables were statistically significant. The unerupted canine group showed better results than the erupted canines group (p=0.001). Cleft lip and alveolus group showed better outcomes than the complete cleft lip and palate group (p = 0.000). The larger the alveolar cleft width, the poorer the outcome of the alveolar graft (p = 0.027). The surgeon also had a significant influence on the success of the surgery (p = 0.003 and 0.001). Conclusion: The canine irruption stage, the type and width of cleft lip and palate and the surgeon influenced the outcome of alveolar graft surgeries performed with rhBMP-2.
Kotake, Bruna Gabriela dos Santos. "Avaliação da reparação óssea em defeitos críticos após a aplicação da proteína rhBMP-2 pura e/ou combinada em animais normais e sob efeito do alcoolismo crônico." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/58/58137/tde-04122012-164616/.
Повний текст джерелаAlcoholism is considered a reducer for new bone formation, may leading to osteometabolic disturbance, considering that the rhBMP-2 is a morphogenetic protein known to play an important role in the bone healing processes. The aim of this study was to investigate the action of alcoholism and its effect on the repair of bone defects (DO) performed in rat calvaria after the rhBMP-2 application, pure or combined with a collagen matrix. We used 80 rats divided into eight groups, each one with a waiting period for sacrifice of 4 and 6 weeks after the bone defect (5mm) delineation in the rat skull. The groups were divided into: G1) water \"ad libitum\" + DO, G2) alcohol \"ad libitum\" + DO, G3) water + DO + 5μg pure rhBMP-2, G4) alcohol + DO + 5μg pure rhBMP-2, G5 ) DO + water + collagen sponge carrier, G6) alcohol + DO + collagen sponge carrier, G7) and water + DO + 5μg rhBMP-2/collagen sponge, G8) alcohol + DO + 5μg of rhBMP-2/collagen sponge. The radiographic data were submitted to statistical analysis Kruskal-Wallis and Dunn\'s Multiple, histological data to analysis of variance (ANOVA) and Tukey test. Radiographically, it was found after 6 weeks that the groups treated with rhBMP-2, independently of the carrier use and ethanol administration, more new bone formation (p<0.05), at 4 weeks it was not found statistical difference. For immunohistochemical analysis, ostecalcin (OC), and bone sialoprotein (BPS) were predominant in groups treated with rhBMP-2. For histological quantification of collagen type I fibers, statistical difference between groups was found (p<0.05) with the increasing of these fibers in the groups treated with rhBMP-2 combined with collagen sponge; and quantitative by stereology, aiming to calculate the volume of new bone, it was found higher values for the groups treated with rhBMP-2 pure or combined to the carrier; but for the groups treated with ethanol, it was found higher bone formation in the groups treated with rhBMP-2 associated to the carrier in the periods of 4 and 6 weeks (p <0.001). It was concluded in this experimental model that the carrier was effective for rhBMP-2 delivery, even in the presence of ethanol.
Izadpanah, Ali. "Effect of rhBMP-7 dosing during distraction osteogenesis in wild type mice." Thesis, McGill University, 2011. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=103541.
Повний текст джерелаL'ostéogenèse par distraction (DO) est une technique courante de chirurgie pour l'allongement des os dans le traitement des anomalies des membres, des malformations congénitales, de la perte osseuse traumatique, des infections et des tumeurs malignes. Elle est composée de trois phases principales: la phase de latence, la phase de distraction, et la phase de consolidation. DO amène cependant plusieurs inconvénients. Le principal inconvénient de l'ostéogenèse par distraction est la longue période de solidification. Ainsi, toute tentative visant à raccourcir ce processus serait grandement bénéfique. Les protéines morphogénétiques osseuses (BMP) sont cruciales au développement des os et du cartilage. Il a récemment été démontré que la BMP-7 a un effet bénéfique sur la formation osseuse au cours de distraction osseuse sur des rats. Cependant, il n'existe pas d'études pour trouver la dose optimale d'injection de BMP-7. Par conséquent, nous avons cherché à déterminer la dose optimale d'administration de BMP-7 exogène au cours de DO. À cette fin, DO a été effectuée sur le tibia droit de 79 souris mâle sauvages (C57BL / 6) en utilisant une version miniature du fixateur Ilizarov. Les animaux ont été séparés au hasard en deux groupes principaux en fonction du moment d'euthanasie; 45 souris ont été euthanasiées à la mi-solidification et 34 ont été euthanasiées à la fin de la solidification. Chaque groupe a ensuite été subdivisé en fonction de la dose administrée de BMP-7. Trois doses différentes de BMP-7 ont été injectées sur le site de l'ostéotomie au premier jour de la distraction (0.05μg, 0.10μg, 0.50μg correspondant à 2μg/kg, 4μg/kg et 20μg/kg de BMP-7). Les groupes de contrôle ont été injectés avec de l'eau pure. Des échantillons ont été collectés à deux points dans le temps: 34 jours (mi-solidification) et 51 jours (fin de la solidification). Les échantillons prélevés ont été étudiés à l'aide d'un rayon-X Faxitron, d'un μCT, de l'histomorphométrie statique, d'essais biomécaniques, de l'immunohistochimie, et de l'histologie. Les résultats ont démontré qu'il y a un effet réducteur pour accélérer la formation osseuse au cours de distraction osseuse avant l'augmentation de la dose de BMP-7 au mi-solidification. L'analyse μCT et les paramètres histomorphométrie statiques ont révélé une diminution statistiquement significative du volume osseux/volume des tissus (BV/TV) et de la régénération osseuse avant l'augmentation de la dose de BMP-7. L'évaluation biomécanique au 34ième jours a également démontré une plus grande rigidité et de travail ultime avant rupture à la plus faible dose administrée de BMP-7. D'autre part, au fin de la solidification, les résultats histomorphométriques statiques et μCT ont démontré la plus grande BV/TV à la dose moyenne d'administration de 4μg/kg (30.09 ± 4,076, 1,533 ± 38,73, 27,02 ± 2,684 et 17,68 ± 3,808 pour 2μkg, 4μg/kg, 20μg/kg, et le contrôle, respectivement). De même, la formation de osseuse avait des résultats comparables (4,062 ± 0,4874, 6,969 ± 1,123, 4,946 et 0,6362 ± pour 2μkg, 4μg/kg et 20μg/kg respectivement). Les essais biomécaniques sur les échantillons prélevés au fin de la solidification ont démontré une diminution progressive de la rigidité (63,72 ± 35,03, 45,56 ± 8,045, 28,99 ± 11,22 et 35,49 ± 17,00 pour 2μkg, 4μg/kg, 20μg/kg, et le contrôle, respectivement) et la force ultime avant rupture (13,69 ± 5,149, 2,086 ± 13,12, 8,815 ± 2,972, 8,188 et 3,334 ± pour 2μkg, 4μg/kg, 20μg/kg, et le contrôle, respectivement) après l'augmentation de la dose. En conclusion, les résultats de cette étude suggèrent que la BMP-7 exerce un rôle physiologique important dans la régénération osseuse au cours de la DO. Également, nous avons démontré qu'avec l'augmentation de la dose administrée, la quantité de formation osseuse et de BV sont à la baisse. Ces résultats sont comparables avec les évaluations biomécaniques obtenus à mi-solidification et fin de la solidification.
Schwartz, Daniel. "Development of an Aqueous Suspension of Recombinant Human Bone Morphogenetic Protein-2 (rhBMP-2)." Diss., lmu, 2005. http://nbn-resolving.de/urn:nbn:de:bvb:19-44316.
Повний текст джерелаJohnson, Trenton. "Effect of guided bone regeneration with rhBMP-2 on bone quality surrounding dental implants." The Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu152147382891412.
Повний текст джерелаLucia, Conrado Ingraci de. "Análise quantitativa dos níveis de cálcio, Colagenase A e B durante o reparo ósseo em calvárias de ratos sob o modelo experimental de defeito ósseo." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/58/58137/tde-03042013-163708/.
Повний текст джерелаBone is a special tissue with a high mineral content and performs various functions in the body, such as calcium reserves, protection of vital structures and muscles lever during the movement. Bone constantly undergoes remodeling processes, which keeps its functional structure and repair small fractures that commonly occur due to the stress of continuous use. The repair system works perfectly synchronized by the cells that produce bone components and resorbing cells, allowing the perfect tissue organization. This maintenance system depends on the interaction between these cells and the signals sent by mediators and modulators. Several proteins operate to induce bone formation, but also to facilitate the reconstruction. Among these proteins are the BMPs, which have great osteoinductive potential, and MMPs that act at different stages of construction and maintenance of this tissue. Particularly BMP-2 has shown significant potential in terms of induction and its recombinant form, rhBMP-2, produced by recombinant engineering, has been released for clinical use and commercialization. In relation to MMPs, there are important functions of MMP-2 and MMP-9 in this tissue. First, structuring the matrix and modulating the resorption during inflammatory processes inherent to repair; second, acting at early to later stages, produced mainly by osteoclasts and used during bone remodeling. However, this repair capacity is limited and large bone defects require a lot of strength of the body, may leading to a bone repair not well structured. Thus, several proposed techniques to stimulate the development and use of bone grafts were effective to provide a framework for growth, facilitating the implementation of new bone and protecting the defect bed throughout the extended recovery period. This study focused on three different types of bone graft (autologous, homologous and heterologous) and their association with rhBMP-2 protein, evaluating the biochemical aspects according to the amount of MMP-2 and MMP-9 in two different periods of time. In general, it was found that firstly, there is an increased production of MMP-2, and MMP-9 levels remain relatively constant in both considered periods of time. The autologous graft presented the best results followed by homologous and heterologous, respectively; however the addition of rhBMP-2 in these grafts did not seem to influence the MMP-2 and MMP-9 levels, in both periods of time. The calcium dosage revealed more mineralization at the autologous and homologous groups, the other groups, besides having lower calcium levels, decreased these levels at the second period of this experiment.
Carvalho, Roberta Martinelli. "Reparo do defeito alveolar com proteína morfogenética óssea (rhBMP-2) em pacientes com fissura labiopalatina." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/61/61132/tde-14022012-100227/.
Повний текст джерелаObjective: The purpose of this study is to assess the bony regeneration of alveolar clefts using bone morphogenetic protein (rhBMP-2) in a collagen sponge carrier. Material and Methods: Twenty four patients with unilateral complete cleft lip and palate, in the very best moment, were randomly assigned to groups control (iliac crest cancellous bone) and test (rhBMP-2 in an absorbable collagen sponge). They were evaluated 6 months and 1 year postoperative with periapical radiographs and computed tomographs using Chelsea scale. Results: For control group, radiographic examination revealed success index for bone height 81,82% 6 months and 83,33% 1 year postoperative; for test group, findings were 91,67% first control and 100% 1 year. Computed tomograph revealed 70% 6 months and 75% 1 year for control group, 91,67% first control and 100% 1 year for bone height test group. Volume ratios were 45,34% bone filling 6 months and 53,33% 1 year for control group and 40,63% 6 months and 53,1% 1 year for test. There was no statistical significance. Conclusion: rhBMP-2 in a collagen sponge carrier induced bony regeneration close to that from iliac crest cancellous bone to repair complete alveolar clefts.
Grella, Benedikt. "Ss-Tricalziumphosphat zur rhBMP-2-induzierten Knochenaugmentation in supraalveolären periimplantären Defekten : eine experimentielle Studie am Hund /." Aachen : Mainz, 2006. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=016991643&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.
Повний текст джерелаBISWAS, DEBLINA. "A BIORELEVANT IN VITRO MODEL TO CHARACTERIZE IN VIVO RELEASE OF BONE MORPHOGENETIC PROTEIN-2 (rhBMP-2)." VCU Scholars Compass, 2017. http://scholarscompass.vcu.edu/etd/4977.
Повний текст джерелаVölschow, Chiara Catharina [Verfasser]. "Präfabrikation von vaskularisierten Transplantaten mittels rhBMP-2 und VEGF165 im Omentum majus des Kaninchens / Chiara Catharina Völschow." Kiel : Universitätsbibliothek Kiel, 2017. http://d-nb.info/1149512776/34.
Повний текст джерелаYong, Mostyn R. "Characterisation of Polycaprolatone-Based Scaffold Plus Recombinant Human Morphogenetic Protein - 2 (RHBMP-2) in an Ovine Thoracic Spine Model." Thesis, Queensland University of Technology, 2013. https://eprints.qut.edu.au/64093/1/Mostyn_Yong_Thesis.pdf.
Повний текст джерелаRosa, Anderson Paim. "Influência do laser em baixa intensidade associado à proteína osteoindutora rhBMP-2 no reparo de defeitos ósseos em calvária de ratas Wistar." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/58/58137/tde-12082013-135234/.
Повний текст джерелаLow-level laser irradiation (LLLI) and recombinant human bone morphogenetic protein type 2 (rhBMP-2) have been used to stimulate bone formation. LLLI stimulates proliferation of osteoblast precursor cells and cell differentiation and rhBMP-2 recruits osteoprogenitor cells to the bone healing area. This in study evaluated the effects of LLLI and rhBMP-2 on the bone healing process in calvaria of female Wistar rats. Critical bone defects were created in the parietal bone in 42 animals, and the animals were divided into six treatment groups with 7 animals: Group 1 - laser in a single application; Group 2- 7 μg of pure rhBMP-2; Group 3 - laser in a single application and 7 μg of pure rhBMP-2; Group 4 - 7 μg of rhBMP-2 combined with monoolein gel, Group 5 - laser in a single application and 7 μg of rhBMP-2 combined with monoolein gel; and Group 6 - critical bone defect controls. A gallium-aluminumarsenide diode laser was used (GaAlAs; wavelength 780 nm, output power 60mW, beam area 0.04 cm2, irradiation time 80 s, energy density 120 J/cm2, irradiance 1.5 W/cm2). After 15 days, the calvarial tissues were removed for histomorphometric analysis. Group 3 defects showed higher amounts of newly formed bone (37.89%) than the defects of all the other Groups (p<0.05). The amounts of new bone in defects of Groups 1 and 4 were not significantly different from each other (24.00% and 24.75%, respectively), but were significantly different from the amounts in the other Groups (p<0.05). The amounts of new bone in the defects of Groups 2 and 5 were not significantly different from each other (31.42% and 31.96%, respectively), but were significantly different from the amounts in the other Groups (p<0.05). Group 6 defects had 14.10% new bone formation, and this was significantly different from the amounts in the other Groups (p<0.05). It can be concluded that LLLI administered during surgery effectively accelerated healing of critical bone defects filled with pure rhBMP-2, achieving a better result than LLLI alone or the use of rhBMP-2 alone.
Beier, So-Young [Verfasser]. "Untersuchungen zur knöchernen Fusion unter Verwendung von rhBMP-2 bei der lumbalen dorsoventralen Spondylodese in PLIF-Technik / So-Young Beier." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2013. http://d-nb.info/1043197729/34.
Повний текст джерелаRaschke, David [Verfasser]. "Knochenregeneration chronischer Knochendefekte der porcinen Maxilla unter kombinierter Freisetzung von rhBMP-2 und rhVEGF-A165 aus PDLLA/CaCO3-composite-Granula / David Raschke." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2020. http://d-nb.info/1220080667/34.
Повний текст джерелаMones, Del Pujol Erwan de. "Reconstruction mandibulaire segmentaire selon la technique des membranes induites avec greffe d’un biomatériau phosphocalcique, de moelle osseuse totale et de simvastatine." Thesis, Bordeaux, 2015. http://www.theses.fr/2015BORD0322/document.
Повний текст джерелаInduced membrane technique with a polymethylmethacrylate (PMMA) spacer and autologous cancellous bone graft has been proposed by doctor Masquelet for segmental long bone reconstruction. This two stage technique could be proposed for segmental mandibular bone reconstruction in oncological situations in case of failure or contraindication of revascularized autologous bone graft. The first objective of this study was to evaluate the histological and biological properties of membranes induced by PMMA with radiotherapy, and to compare them to membranes induced by silicone. This material smoother than PMMA has been chosen to facilitate spacer removal. Membranes induced by both materials in subcutaneous models in rats had similar histological and biological properties, but membranes induced by silicone were less affected by radiotherapy. The second objective of this study was to propose a tissue engineering procedure as an alternative to autologous bone graft. The new bone formation inside silicone induced membranes has been analyzed after implantation of different combinations of macroporous biphasic phosphocalcic ceramic (MBCP+™), total bone marrow, simvastatine and rhBMP-2 in subcutaneous and femoral osseous models in rats. No significant new bone formation has been demonstrated in simvastatin groups. However, a significant new bone formation has been demonstrated in rhBMP-2 groups, with or without radiotherapy. An increased new bone formation has also been demonstrated with total bone marrow
Jernberg, Cassandra. "A Computational Model for Fracture Healing Integrated with Mechanical Stimulation and Growth Factors." Thesis, KTH, Skolan för teknik och hälsa (STH), 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-143536.
Повний текст джерелаCruz, Eden E. "THE EFFECT OF RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN-2 ON THE OSSEOINTEGRATION OF TEMPORARY ANCHORAGE DEVICES." DigitalCommons@CalPoly, 2010. https://digitalcommons.calpoly.edu/theses/331.
Повний текст джерелаMoraes, Paulo Hemerson de 1982. "Rehabilitation of fully edentulous maxilla = retrospective survival analysis of dental implants in native x autogenous bone and proposed technique for bone reconstruction with rhBMP-2 = Reabilitações de maxilas totalmente edêntulas: análise retrospectiva de sobrevida de implantes dentários em osso nativo x enxerto autógeno e proposta de técnica para reconstrução óssea com rhBMP-2." [s.n.], 2013. http://repositorio.unicamp.br/jspui/handle/REPOSIP/289417.
Повний текст джерелаTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
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Resumo: Em maxilas severamente absorvidas, como tratamento de reabilitação, podem ser utilizados os implantes dentais osseointegráveis convencionais. Todavia, nestas situações, estão presentes obstáculos para a instalação dos implantes como: quantidade insuficiente e qualidade inadequada do osso encontrado na maxila. Nestas situações, são necessárias cirurgias reconstrutivas para restabelecer as dimensões do rebordo avelolar em espessura e altura possibilitando assim a instalação adequada destes implantes. Geralmente, essas reconstruções utilizam osso autógeno retirado de algum sitio doador do paciente, como a calota craniana, costela e crista do ilíaco. Alternativamente, com os avanços da engenharia tecidual, a rhBMP-2 (recombinant human bone morphogenethic protein-2) surgiu eliminando a necessidade de qualquer remoção de sítio doador como também qualquer outro biomaterial nas reconstruções ósseas dos maxilares deixando o procedimento com menor morbidade e maior aceitabilidade dos pacientes. Desta forma, a presente pesquisa apresenta 2 estudos descritos nos capítulos a seguir. CAPÍTULO 1: O objetivo deste estudo foi avaliar a sobrevida de implantes instalados em osso nativo de maxilas como também nas reconstruídas com osso autógeno. Foi obtido um acompanhamento de 8-10 anos com pacientes de ambos os grupos reabilitados com próteses fixas sobre implantes dentais. O sucesso dos implantes em maxilas reconstruídas com ósso autogeno não apresentaram diferenças estatísticas à implantes inseridos em maxilas com osso nativo. CAPÍTULO 2: O objetivo deste estudo foi apresentar uma nova técnica de reconstrução óssea de maxila utilizando rhBMP-2 objetivando previsibilidade e melhores resultados no aumento do volume ósseo necessário. A técnica sugerida ofereceu bons resultados no volume ósseo a ser obtido nas reconstruções ósseas de maxila com rhBMP-2.
Abstract: In maxillary severely absorbed as rehabilitation treatment, can be used conventional dental implants. However, these situations are present obstacles to the installation of the implants as insufficient and inadequate quality of bone found in the jaw , as well as the expansion of the maxillary sinus pneumatization . In these situations, it is necessary reconstructive surgery to restore the dimensions of thickness and height on rim thus allowing proper installation of these implants. Generally, these reconstructions using autogenous bone taken from a donor site from the patient , such as calota craneal , rib and the iliac crest . Alternatively, with advances in tissue engineering, rhBMP-2 (recombinant human bone morphogenethic protein-2) appeared eliminating the need to remove any donor site as well as any other biomaterial in the maxila bone reconstruction procedure with leaving the lower morbidity and higher patient acceptability. Given the above, this research presents two studies described in the following chapters. CHAPTER 1: The objective of this study was to evaluate the survival of implants placed in native bone in the maxilla as well as in maxilla reconstructed with autogenous bone. We obtained a follow-up of 8-10 years with both groups of patients rehabilitated with fixed prostheses on dental implants. The survival of implants in maxilla was reconstructed with autogenous bone implants inserted into the lower maxilla with native bone. CHAPTER II: The objective of this study was to present a new technique for reconstruction of maxilla bone using rhBMP-2 seeking precision in increased bone volume needed. The suggested technique offered precision in bone volume to be obtained in the maxilla bone reconstruction with rhBMP-2.
Doutorado
Cirurgia e Traumatologia Buco-Maxilo-Faciais
Doutor em Clínica Odontológica
Jedelhauser, Claudia Catrine [Verfasser], and Matthias [Akademischer Betreuer] Schieker. "Osteogene Differenzierung von humanen mesenchymalen Stammzellen in thermoresponsiven Pluronic® P123–Hydrogelen mit Osteoinduktion durch den Wachstumsfaktor rhBMP-7 / Claudia Catrine Jedelhauser ; Betreuer: Matthias Schieker." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2018. http://d-nb.info/1177681943/34.
Повний текст джерелаBissinger, Marc Oliver [Verfasser], Andreas Akademischer Betreuer] Kolk, and Klaus-Dietrich [Akademischer Betreuer] [Wolff. "Bildgebende Beurteilung des Knochenwachstums nach Applikation von rhBMP-2 versus Nukleinsäurentransfer am Rattenunterkiefer / Marc Oliver Bissinger. Gutachter: Andreas Kolk ; Klaus-Dietrich Wolff. Betreuer: Klaus-Dietrich Wolff." München : Universitätsbibliothek der TU München, 2011. http://d-nb.info/1013435915/34.
Повний текст джерелаGoldstein, Jan [Verfasser], Henning [Akademischer Betreuer] Schliephake, Nicolai [Gutachter] Miosge, and Martin [Gutachter] Oppermann. "Induktion der Knochenneubildung durch Implantation von mit rhBMP-2 und VEGF beladenen Polylaktidträgern im ektopen Tiermodell / Jan Goldstein ; Gutachter: Nicolai Miosge, Martin Oppermann ; Betreuer: Henning Schliephake." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2016. http://d-nb.info/1124680322/34.
Повний текст джерелаCruz, Erin E. "OSSEOINTEGRATION OF TEMPORARY ANCHORAGE DEVICES USING RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN-2." DigitalCommons@CalPoly, 2010. https://digitalcommons.calpoly.edu/theses/343.
Повний текст джерелаPichotano, Elton Carlos [UNESP]. "Avaliação das alterações no nível da crista óssea e na estabilidade de implantes em função instalados na região anterior da maxila enxertada com osso autógeno ou rhBMP-2." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/127579.
Повний текст джерелаCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
A reabilitação oral com prótese sobre implantes osseointegrados tem se mostrado um procedimento seguro e de alta previsibilidade. Porém, trabalhos longitudinais correlacionando a osseointegração de implantes instalados em áreas previamente preenchidas por biomateriais e a taxa de sucesso alcançada por estes implantes após carregamento protético são menos comuns e, muitas vezes, pouco conclusivos. O presente estudo teve como objetivo avaliar o sucesso a curto prazo de implantes instalados na região anterior edêntula da maxila enxertada com osso autógeno intraoral ou com rhBMP-2 após carregamento protético convencional, por meio de parâmetros clínicos e radiográficos. Pacientes com implantes instalados em áreas enxertadas com osso autógeno (grupo controle, n = 12) ou rhBMP-2 (grupo teste, n = 10) foram submetidos à reabilitação protética e monitorados pelo período de 6 meses. Foram mensurados a estabilidade dos implantes através da análise de frequência por ressonância (ISQ) e as alterações no nível da crista óssea marginal através de radiografias periapicais padronizadas. Também foram avaliados os parâmetros clínicos de índice de placa, índice de sangramento marginal, profundidade de sondagem e sangramento à sondagem. Os valores de ISQ mensurados antes da instalação das próteses e após 6 meses de função foram semelhantes para os grupos e não houve diferença entre valores ISQ dentro de cada grupo. Não foram observadas diferenças significativas entre os grupos com relação aos parâmetros clínicos avaliados. Na avaliação do nível ósseo marginal, não foram encontradas diferenças significativas entre os diferentes períodos de avaliação dentro de cada grupo. A perda óssea marginal nos grupos controle e teste foi de 0,28 e 0,12 mm após 3 meses de função e 0,15 e 0,08 mm após 6 meses, sem diferença significativa(Resumo completo, clicar acesso eletrônico abaixo).
Oral rehabilitation with osseointegrated implants has shown to be a safe and highly predictable procedure. However, prospective longitudinal studies correlating osseointegration in previously grafted areas with the success rate achieved by these implants after loading are less common and often inconclusive. The aim of present study was to evaluate short-term success of implants placed in anterior edentulous maxilla grafted with intraoral autogenous bone graft or rhBMP- 2 after conventional prosthetic loading, through clinical and radiographic parameters. Patients with dental implants installed in areas grafted with autogenous bone (control group, n = 12) or rhBMP-2 (test group, n = 10) were submitted to prosthetic rehabilitation and monitored for 6 months. The implant stability and the changes in marginal bone level were measured. The following clinical parameters were evaluated: plaque index, marginal bleeding index, probing depth and bleeding on probing. The ISQ values measured before and 6 months after loading were similar between the groups. There was no significant difference between ISQ values within each group. No significant differences with respect to clinical parameters were detected between the groups. Regarding the marginal bone level measurement, no significant differences could be verified between the different periods of evaluation within each group. The marginal bone loss in the test and control groups was 0,28 and 0,12 mm after 3 months of loading and 0,15 and 0,08 mm after 6 months, without significant difference between the groups. It was concluded that the implants installed in the anterior edentulous maxilla grafted with rhBMP- 2 had similar short-term success rate than the implants installed in areas grafted with intraoral autogenous bone graft.
Pichotano, Elton Carlos. "Avaliação das alterações no nível da crista óssea e na estabilidade de implantes em função instalados na região anterior da maxila enxertada com osso autógeno ou rhBMP-2/." Araraquara, 2014. http://hdl.handle.net/11449/127579.
Повний текст джерелаCo-orientador: Elcio Marcantonio Junior
Banca: Andréa Cândido dos Reis
Banca: José Maurício dos Santos Nunes Reis
Resumo: A reabilitação oral com prótese sobre implantes osseointegrados tem se mostrado um procedimento seguro e de alta previsibilidade. Porém, trabalhos longitudinais correlacionando a osseointegração de implantes instalados em áreas previamente preenchidas por biomateriais e a taxa de sucesso alcançada por estes implantes após carregamento protético são menos comuns e, muitas vezes, pouco conclusivos. O presente estudo teve como objetivo avaliar o sucesso a curto prazo de implantes instalados na região anterior edêntula da maxila enxertada com osso autógeno intraoral ou com rhBMP-2 após carregamento protético convencional, por meio de parâmetros clínicos e radiográficos. Pacientes com implantes instalados em áreas enxertadas com osso autógeno (grupo controle, n = 12) ou rhBMP-2 (grupo teste, n = 10) foram submetidos à reabilitação protética e monitorados pelo período de 6 meses. Foram mensurados a estabilidade dos implantes através da análise de frequência por ressonância (ISQ) e as alterações no nível da crista óssea marginal através de radiografias periapicais padronizadas. Também foram avaliados os parâmetros clínicos de índice de placa, índice de sangramento marginal, profundidade de sondagem e sangramento à sondagem. Os valores de ISQ mensurados antes da instalação das próteses e após 6 meses de função foram semelhantes para os grupos e não houve diferença entre valores ISQ dentro de cada grupo. Não foram observadas diferenças significativas entre os grupos com relação aos parâmetros clínicos avaliados. Na avaliação do nível ósseo marginal, não foram encontradas diferenças significativas entre os diferentes períodos de avaliação dentro de cada grupo. A perda óssea marginal nos grupos controle e teste foi de 0,28 e 0,12 mm após 3 meses de função e 0,15 e 0,08 mm após 6 meses, sem diferença significativa(Resumo completo, clicar acesso eletrônico abaixo).
Abstract: Oral rehabilitation with osseointegrated implants has shown to be a safe and highly predictable procedure. However, prospective longitudinal studies correlating osseointegration in previously grafted areas with the success rate achieved by these implants after loading are less common and often inconclusive. The aim of present study was to evaluate short-term success of implants placed in anterior edentulous maxilla grafted with intraoral autogenous bone graft or rhBMP- 2 after conventional prosthetic loading, through clinical and radiographic parameters. Patients with dental implants installed in areas grafted with autogenous bone (control group, n = 12) or rhBMP-2 (test group, n = 10) were submitted to prosthetic rehabilitation and monitored for 6 months. The implant stability and the changes in marginal bone level were measured. The following clinical parameters were evaluated: plaque index, marginal bleeding index, probing depth and bleeding on probing. The ISQ values measured before and 6 months after loading were similar between the groups. There was no significant difference between ISQ values within each group. No significant differences with respect to clinical parameters were detected between the groups. Regarding the marginal bone level measurement, no significant differences could be verified between the different periods of evaluation within each group. The marginal bone loss in the test and control groups was 0,28 and 0,12 mm after 3 months of loading and 0,15 and 0,08 mm after 6 months, without significant difference between the groups. It was concluded that the implants installed in the anterior edentulous maxilla grafted with rhBMP- 2 had similar short-term success rate than the implants installed in areas grafted with intraoral autogenous bone graft.
Mestre
Cruz, Ariadne Cristiane Cabral da. "Avaliação in vitro e in vivo dos efeitos da proteína óssea morfogenética tipo 2 recombinante (rhBMP-2) e de células-tronco derivadas do tecido adiposo humano na osteogênese." reponame:Repositório Institucional da UFSC, 2012. http://repositorio.ufsc.br/xmlui/handle/123456789/100605.
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Dentre os vários fatores de crescimento que têm sido relatados na literatura para tratamento de defeitos ósseos, as proteínas ósseas morfogenéticas (BMPs) têm atraído bastante interesse, em decorrência de sua capacidade osteoindutora. As células-tronco derivadas do tecido adiposo (ASCs) também vêm se destacando na regeneração tecidual e na modulação da resposta inflamatória pelo seu efeito parácrino. O objetivo deste trabalho foi avaliar in vitro e in vivo os efeitos da BMP-2 e de ASCs na osteogênese. Os experimentos se dividiram em 4 etapas, sendo: (1) avaliação do efeito da proteína óssea morfogenética recombinante humana do tipo 2 (rhBMP-2), da BMP-4 e da BMP-7 na diferenciação osteogênica de ASCs suplementadas com arcorbato e ?-glicerofosfato; (2) comparação do efeito do ácido retinóico (AR), da rhBMP-2 e da rhBMP-2+AR na diferenciação osteogênica de ASCs; (3) avaliação do efeito da implantação de ASCs incorporadas em arcabouços de poli(ácido láctico-co-glicólico) (PLGA) na inflamação causada por rhBMP-2 no tecido muscular de cães; e (4) avaliação do efeito de ASCs incorporadas em arcabouços autógenos de plasma rico em plaquetas (PRP) na regeneração óssea na tíbia de cães. Os resultados confirmaram as caracterísitcas de células-tronco mesenquimais das ASCs, através da imunofenotipagem e da diferenciação adipogência e osteogênica. Em relação à etapa 1, as ASCs expressaram BMP-4 e BMP-7 endógenas, a suplementação do meio osteogênico com rhBMP-2 não aumentou a atividade de ALP, a expressão do mRNA da osteonectina e osteocalcina, e a deposição de cálcio. No que diz respeito à etapa 2, a rhBMP-2 promoveu maior expressão de Smad 1 e BMP-7 (dia 7), maior expressão relativa do mRNA do receptor de BMP do tipo II (BMPR-II) e do mRNA da osteocalcina (dia 21). Por sua vez, o AR exibiu as maiores atividades de ALP (dias 7, 14, 21 e 28), maior expressão relativa do mRNA do receptor de BMP do tipo II (RBMP-II) (dia 14), do mRNA da Smad 1 (dia 21) e do mRNA da osteonectina (dias 14, 21 e 28). A associação rhBMP-2+AR promoveu os maiores níveis de cálcio na matriz extra-celular (dias 12 e 32), a expressão da Smad 4 considerável (dias 21 e 28), expressão da Smad 1/ 5/ 8 fosforilada similar ao MC3T3-E1 (controle positivo) (dias 7, 14, 21 e 28), maior expressão relativa do mRNA da Smad 1, mRNA da osteocalcina e mRNA da osteonectina (dias 14, 7 e 7, respectivamente). Em relação à etapa 3, a agregação da rhBMP-2 aos arcabouços foi de aproximadamente 78, 74 e 61% para 1, 2,5 e 5 µg de rhBMP-2.mL-1, respectivamente. A liberação acumulada da rhBMP-2, em 21 dias, foi de aproximadamente 90, 64 e 64% para 1, 2,5 e 5 µg de rhBMP-2.mL-1, respectivamente. Os arcabouços de PLGA foram quase que totalmente degradados em 6 semanas de pós-operatório. As ASCs foram capazes de modular a resposta inflamatória causada por baixa dose de rhBMP-2 (2,5?g) no tecido muscular de cães, diminuindo o número de focos inflamatórios, células gigantes e aumentando a neovascularização. Os resultados da etapa 4 mostraram que PRP+ASCs apresentou menor quantidade de tecido de granulação e promoveu maior quantidade (osso primário e secundário) e maturação (osso secundário) de tecido ósseo neoformado nos defeitos ósseos, comparado com osso autógeno e PRP, isoladamente. Resumidamente, pode-se concluir que a suplementação da rhBMP-2 ao meio osteogênico não melhorou a osteogênese das ASCs; a associação de rhBMP-2+AR se mostrou mais eficiente na osteodiferenciação das ASCs, comparado com rhBMP-2 ou AR isoladamente; as ASCs reduziram o processo inflamatório e aumentaram a neovascularização nos sítios musculares que receberam baixa dose de rhBMP-2; e as ASCs associadas com PRP apresentaram menor quantidade de tecido de granulação e promoveram maior quantidade e maturação de tecido ósseo neoformado nos defeitos ósseos, comparado com osso autógeno e PRP.
Abstract : Recombinant human bone morphogenetic protein type 2 (rhBMP-2) is a potent osteogenic growth factor, which has been used for treatment of bone defects in maxillofacial, oral, orthopedic, and spine surgeries. The adipose-derived stem cells (ASCs) have been draun attention in tissue regeneration and modulation of inflamatory disease because of their paracrine effect. This study evaluated the in vitro and in vivo effects of rhBMP-2 and ASCs in osteogenesis. The experiments were divided into 4 parts: (1) evaluated the effect of recombinant human bone morphogenetic protein type 2 (rhBMP-2), BMP-4, and BMP-7 in osteogenic differentiation of ASCs suplemented with arcorbate and â- glycerophosphate; (2) compared the effect of retinoic acid (RA), rhBMP-2, and rhBMP-2+AR in osteogenic differentiation of ASCs; (3) evaluated the effect of the implantation of ASCs incorporated into scaffolds of poly lactic-co-glycolic (PLGA) in canine muscular tissue; and (4) evaluated the effect of ASCs incorporated into scaffolds of platelet-rich plasm (PRP) in bone regeneration in canine tibia. The results confirmed the mesenchymal stem cells characteristics of ASCs. According to part 1, ASCs expressed endogenous BMP-4 and BMP-7. The supplementation of osteogenic medium with rhBMP-2 did not increase ALP activity, mRNA expression of osteonectin and osteocalcin, and the calcium deposition. According to the results of part 2, rhBMP-2 promoted the highest expression of Smad1 and BMP-7 (day 7), the highest relative expression of BMP receptor type II (BMPR-II) mRNA and osteocalcin mRNA (day 21). RA exhibited the highest phosphatase alkaline activity (ALP) (days 7, 14, 21 and 28), highest expression of BMPR-II mRNA (day 14), Smad 1 mRNA (day 21) and osteonectin mRNA (days 14, 21, and 28). The association rhBMP-2+RA promoted the highest levels of calcium in extracellular matrix (days 12 and 32), a considerable expression of Smad 4 (days 21 and 28), expression of phosphorylated Smad 1/ 5/ 8 similar to MC3T3-E1 (positive control) (days 7, 14, 21, and 28), the highest expression of Smad 1 mRNA, osteocalcin mRNA, and osteonectin mRNA (days 14, 7, and 7, respectivamente). According to part 3, the rhBMP-2 loading into the scaffolds was approximately 78, 74, and 61% for 1, 2.5, and 5 ìg of rhBMP-2.mL-1, respectively. Cumulative rhBMP-2 release was approximately 90, 64, and 64% for 1, 2.5, and 5 ìg of rhBMP-2.mL-1, respectively, at day 21. PLGA scaffolds were almost completely degradated at 6 weeks postoperative. ASCs modulated inflammatory response induced by low dose of rhBMP-2 (2.5ìg) in muscle sites, decreasing the number of inflammatory foci, giant cells, and neovascularization. The results of part 4 showed that PRP+ASCs promoted the fewest formation of granulation tissue, the highest amount of bone neoformation (primary and secondary bone), and the highest level of bone maturation in bone deffects, compared to autogenous bone and PRP, alone. In summary, supplementation of osteogenic medium with rhBMP-2 did not improve the osteogenic differentiation of ASCs; the assotiation rhBMP-2+RA was more efficient on osteodifferentiation process compared to rhBMP-2 or RA alone; ASCs modulated the inflammatory process and increased neovascularization in muscle sites with low dose of rhBMP-2; the assotiation ASCs+PRP promoted the lowest number of granulation tissue and increased the amount of bone neoformation (primary and secondary bone) and maturation in bone deffects, compared to autogenous bone and PRP, alone.
Palone, Marcos Roberto Tovani. "Efeito de um dentifrício com digluconato de clorexidina a 0,12% sobre a saúde bucal durante o período pós-operatório de pacientes submetidos à cirurgia de enxerto alveolar secundário com rhBMP-2." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/61/61132/tde-08042015-164134/.
Повний текст джерелаIndividuals with cleft lip and palate often present difficulty to perform adequate oral hygiene, presenting greater dental plaque accumulation with consequent microbial imbalance in the oral cavity; therefore, they were clinically considered high risk individuals for dental caries and periodontal disease. Plaque control is particularly important in the postoperative period after secondary alveolar graft, aiming to avoid postoperative infection, maintain the periodontal health and consequently promote better repair. This study evaluated the effectiveness of a dentifrice containing 0.12% chlorhexidine digluconate on the oral health of individuals with cleft lip and palate during the postoperative period after secondary alveolar graft. A double blind study was conducted on 20 individuals randomly divided in two groups (A and B). Individuals in group A (control) received conventional dentifrices, and individuals in group B received a dentifrice containing 0.12% chlorhexidine digluconate. Individuals in both groups received small head toothbrushes with extra-soft bristles, as well as oral hygiene instructions for toothbrushing three times a day (at morning, after lunch and before sleep). The individuals were analyzed in two periods, namely preoperative and late postoperative (after three months), comprising evaluation of gingival index, plaque index, DMFT, dmft, tooth staining, as well as the occurrence of taste changes. Statistical analysis for the variables gingival index, plaque index, DMFT and dmft for both groups revealed no statistically significant difference between groups, and between the study periods for both groups. Only one individual, in group A, reported taste changes during the study period. No tooth staining indicating association with the utilization of chlorhexidine was observed in the clinical examinations. This study highlighted the importance and positive influence of individualized care for toothbrushing instructions, especially considering that this surgical procedure is usually performed in children or pre-teens, under responsibility of proper accomplishment of postoperative care measured by their caregivers.
Brancaz, Maud-Virginie. "Analyse cellulaire et moléculaire de la mutation "bald Mill Hill" (hr rhbmh) au cours du développement chez la souris." Université Joseph Fourier (Grenoble), 2004. http://www.theses.fr/2004GRE10097.
Повний текст джерелаIt is well documented that the mammalian hairless gene plays a critical role in the maintenance of hair growth. The hairless mutant ph enotype is characterised by a progressive and definitive hair loss. Although the hr gene has been known for many years, the biochemical function of the encoded Hairless (Hr) remains unclear. Hr encodes a putative zinc finger protein of approximately 127 kDa acting as a corepressor of nuclear hormone receptors. It interacts with histone deacetylases (HDACs) and is localized in the nuclear Matrix-Associated Deacetylase (MAD) bodies. Allelic mutations in the murine hairless gene, which arose spontaneoulsy in a colony at the National Institute for Medical research (NIMR), Mill Hill, London in early 1998. The gene symbol hr rhbmh mice revealed a substantial deletion at the 3'-end of the gen. Our results indicate that the transcription of the gene is surprisingly unaffected by the deletion. Instead it results in ablation of the determination codon leading to a new protein. This protein encompasses a 117 amino acids extension at its C-terminal part and presents an abnormal cellular localization. In an attempt to identify partners and targets of the hairless gene, we have analysed a series of markers of hair follicle and epidermal development in these mutant mice
Boven, Johanna Margaretha [Verfasser], Henning [Akademischer Betreuer] Schliephake, Nicolai [Gutachter] Miosge, and Rainer [Gutachter] Mausberg. "Einfluss der kombinierten Freisetzung von rhBMP-2 und rhVEGF165 aus PDLLA/Calciumcarbonat-Gerüsten auf die In-vitro-Aktivität der Osteogenese und Angiogenese / Johanna Margaretha Boven ; Gutachter: Nicolai Miosge, Rainer Mausberg ; Betreuer: Henning Schliephake." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2018. http://d-nb.info/1161606610/34.
Повний текст джерелаCirano, Fabiano Ribeiro. "Influência de diferentes superfícies de titânio na adesão, proliferação e diferenciação de células semelhantes a osteoblastos de ratos (osteo-1) em culturas, na presença ou não da proteína morfogenética óssea recombinante-2 (rhBMP-2)." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/23/23146/tde-08042008-180815/.
Повний текст джерелаThis study has analyzed the influence of different titanium surfaces in the adhesion, proliferation and differentiation of rat osteoblast-like cells (osteo-1 culture), in the presence or not, of the recombinant bone morphogenetic protein-2 (rhBMP-2). The osteo-1 cells were grown on the following titanium surfaces: 1. smooth surface; 2. coarse grit-blasted and acid-etched surface (SLA); and 3. coarse grit-blasted and acid-etched surface under nitrogen protection, and stored in sodium chloride isotonic solution (SLActive), in the presence or not, of 20 ng/ml of rhBMP-2. It was analyzed the cell adhesion in 24 hours, the total protein content, the collagen content, and the alkaline phosphatase in 7, 14 and 21-day periods, and also the formation of calcified nodules in 21 days. The results showed that the adhesion was neither influenced by the surface type, nor by the treatment with rhBMP-2 (p=0.0936). When we related the total protein content to the total number of cells, we noticed that the proliferation was not influenced by the titanium surface type; however, the addition of rhBMP-2 led to a statistically significant reduction on the SLA surface at 21 days (p=0.0000). Concerning the differentiation, we could observe that the surface type did not influence the total content of proteins, the collagen content and the formation of calcified nodules in any of the analyzed periods. The alkaline phosphatase activity was only influenced by the surface type at 14 days, where the group C/SLActive presented lower values than the group C/Smooth (p=0.0000). The addition of rhBMP- 2 promoted a bigger influence over the differentiation process, thus leading to a statistically significant reduction in the total protein content on the SLA surface at 21 days (p=0.0000), a statistically significant increase in the collagen content on the surface SLActive in the 7-day period (p=0.0005), a statistically significant reduction in the alkaline phosphatase activity on the smooth surface in the 14 and 21-day periods, on the SLA surface at 14 days, and on the SLActive surface at 21 days (p=0.0000). Only the formation of calcified nodules did not undergo influence of the rhBMP-2 addition.
Krohn, Sebastian [Verfasser], Rudolf M. [Akademischer Betreuer] Gruber, and Nikolaus [Akademischer Betreuer] Gersdorff. "Die Rekonstruktion des Unterkiefers bei Knochendefekten mit einer Kombination aus rhBMP-2, einer synthetischen Polyethylenglycol-Matrix und Calciumphosphat -Eine Pilotstudie am Göttinger Minipig / Sebastian Krohn. Gutachter: Rudolf M. Gruber ; Nikolaus Gersdorff. Betreuer: Rudolf M. Gruber." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2015. http://d-nb.info/1070686050/34.
Повний текст джерелаTai-I, Li. "Modulation effect of rhBMP2 on osteogenic activity in human periodontal ligament fibroblasts and osteoblast cell lines." 2005. http://www.cetd.com.tw/ec/thesisdetail.aspx?etdun=U0001-2007200508331100.
Повний текст джерелаLi, Tai-I., and 李泰億. "Modulation effect of rhBMP2 on osteogenic activity in human periodontal ligament fibroblasts and osteoblast cell lines." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/40798215143389216558.
Повний текст джерела國立臺灣大學
臨床牙醫學研究所
93
Background: Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently under way to evaluate the potential of BMPs to promote fracture-healing and bone regeneration. The aim of this study was to investigate the biological effects of rhBMP2 on primary cultured cells isolated from periodontal ligament, gingival tissue, cancellous bone of human specimens and bone marrow aspirate before clinical trial of rhBMP2 in periodontal tissue engineering. Methods: We analyzed effect of different concentration of rhBMP2 on osteogenic activity in periodontal ligament fibroblasts (PDLF), gingival fibroblasts (GF), bone-derived cells (HBDC) and bone marrow-derived cells (HBMDC). Cytochemical staining and assay of alkaline phosphatase activity were performed in different cell types. Effect of rhBMP2 stimulation on in vitro mineralization was studied by Alizarin red S stain. Differential modulation of core biding factor 1 (Cbfa1), osteoprotegerin (OPG), and receptor of NF-κB (RANKL) mRNA expression by rhBMP2 were examined by semiquantitative RT-PCR. Results: rhBMP2 was found to stimulate an increase of alkaline phosphatase activity and cytochemical staining which was dose dependent (0-300ng/ml) in PDLF and HBDC. The study mentioned above was not in GF cells. HBMDC showed dramatically increased cytochemical staining at relative low dose of rhBMP2 (50ng/ml). rhBMP2 (300ng/ml) significantly enhanced in vitro mineralization in HBDC and HBMDC, but only had mild effect on PDLF cells in confluent 20-days culture. PDLF cells and HBDC showed the expression of Cbfa1, OPG, but not RANKL at mRNA level, and Cbfa1 mRNA level was up-regulated upon rhBMP2 stimulation. Conclusions: The data of this study support the concept that PDLF cells have phenotypes of osteoblast linage. The fact that rhBMP2 can both increase ALPase activity and promote in vitro mineralization in PDLF cells and HBDC raises the possibility that rhBMP2 may be useful in directing the regeneration of periodontal mineralized tissue during wound healing. Further study using signal such as rhBMP2 in tissue scaffold to modulate PDLF cells differentiation may be needed in tissue engineering of periodontal tissue.
Hsu, Yung Heng, and 徐永衡. "The Implant Enveloped with Antibiotics/PLGA/rhBMP2 Nanofibrous Membrane Sustains to Release High Antibiotic Bactericidal Concentration in New Animal Model." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/wj3kyu.
Повний текст джерела盧主斌. "On the Establishment of Marine Database Pertinent to the Missions of Coast Guard Administration." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/rhbmpu.
Повний текст джерела中央警察大學
水上警察研究所
100
This research provides some suggestions about the establishment of the marine database pertinent to the missions of CGA. We describe the substantial contents of marine database in the first place. The marine databases of advanced countries including the United States of America, Japan and British are introduced later. The current status of domestic marine databases are also presented. Next the marine environmental conditions around Taiwan are described according to the associated historical literatures. The missions of CGA closely related with marine database are outlined before the discussions of the applicability of existing database to the CGA missions. The final conclusion is that CGA should construct a marine database on their own based on the existing resources so as to promote the efficiency of executing CGA missions.
Emmert, Janne. "Vergleich der Osteoinduktivität von rhBMP-2 und rhBMP-2-Mutanten : eine Untersuchung im heterotopen Rattenmodell." Doctoral thesis, 2004. https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-7764.
Повний текст джерелаThe aim of this study was to compare the osteoinductive effect of the BMP mutants T3 and T4 with the human BMP-2 in a rat model (ectopic location). The carrier of BMPs was a collagenous bone matric (ICBM) of standardized size (10mm lenght, 5mm diameter)which were placed in the femur muscle of the rats. The BMP-2 mutants were implanted into the left and rhBMP-2 into the right femur with a concentration of 0,25, 0,5, 1, 2 and 4µg. The presence of the induced bone formation was evaluated radiologically and with an in vivo labelling technique at the day 5, 11, 17, 23 and 28. We prepared sections by the cutting-grinding technique for histological examination. The radiological evaluation showed that the largest bone formation was induced in the group of the BMP-2 mutant T4. T3 only showed a few ratio of new bone. With the concentration of 0,25µg protein no osteoinduction was observed. All morphogenes induced dose-dependent bone formation in muscle tissue of the rats. The size of the observed ossification depended on the amount of implantad BMP-2/T4/T3. The implantation of ICBM-carriers with 4µg BMP-2 or T4 resulted in the ossification of the complete carrier with functional active bone marrow. The study demontrated that the BMP-2 mutant T4 induced heterotopic bone formation at lower concentrations than natural BMP-2 or the T3 mutant
Emmert, Janne [Verfasser]. "Vergleich der Osteoinduktivität von rhBMP-2 und rhBMP-2-Mutanten : eine Untersuchung im heterotopen Rattenmodell / vorgelegt von Janne Emmert." 2004. http://d-nb.info/970170602/34.
Повний текст джерелаSiegrist, Katharina. "Lumbale Spondylodese - Untersuchung der rhBMP-2 bedingten Knochenregeneration im Schafmodell." Doctoral thesis, 2016. https://ul.qucosa.de/id/qucosa%3A15706.
Повний текст джерелаBarr, Torin Richard. "Comparison of the Osteoinductivity of Infuse® and OP-1® via in vitro and in vivo Assays." Thesis, 2009. http://hdl.handle.net/1807/26533.
Повний текст джерелаSmeets, Ralf [Verfasser]. "rhBMP-2 zur Knochenaugmentation bei transgingivaler Implantateinheilung / vorgelegt von Ralf Smeets." 2004. http://d-nb.info/974669296/34.
Повний текст джерелаEmerick, Marcelo. "Levantamento de seio maxilar: tratamento e perspectivas futuras associadas a rhBMP-2." Master's thesis, 2018. http://hdl.handle.net/10284/7222.
Повний текст джерелаThe maxillary sinus lift procedure has been extensively explored for assisting in the process of rehabilitating the upper arch of edentulous patients in the posterior region. The standard surgical technique involves filling the cavity with autogenous bone or synthetic biomaterials, or a combination of these, associated with the principle of osteopromotion, through guided bone regeneration (ROG), with the use of a resorbable membrane. Some authors report that the use of synthetic biomaterials is sufficient for this type of surgery. However, histological analyzes of biopsies performed show that the synthetic materials remain in the maxillary sinus without undergoing the remodeling process. When using the autogenous graft for bone gain in height, the best final results regarding the amount of viable bone are verified. In order to avoid a second surgical site, new materials have been developed and researched. One of these has revolutionized bone treatments and involves the molecular knowledge of bone, this material being known as Bone Morphogenetic Protein - recombinant human-2 (rhBMP2). The effects of this synthetic protein have been verified in procedures of maxillary sinus elevation in order to evaluate their impact on the new bone formation when compared with the autogenous bone. Thus, the present review aims to compare the results described in the literature of maxillary sinus lift procedure with the use of autogenous bone graft and synthetic graft with rhBMP-2.
Hu, Tianyi. "Role of RHBDD2 in membrane trafficking regulation." Master's thesis, 2015. http://hdl.handle.net/10451/22491.
Повний текст джерелаRHBDD2 é parcialmente relacionada com romboides, protéases intra-membranares que degradam substratos na via secretora. Curiosamente, um subgrupo da família romboide, incluindo a RHBDD2, não tem resíduos catalíticos essenciais. Estas “pseudo-proteases” são conservadas, o que implica uma pressão seletiva para manter a sua função durante a evolução. Recentemente, uma mutação pontual (R85H) foi identificada no gene humano que codifica para a proteína RHBDD2, e está associada a uma doença neuro-degenerativa chamada Retinitis Pigmentosa (RP). Esta mutação pontual de G para A, alterou o aminoácido codificado de arginina para histidina no códão 85 do Segundo exão. No entanto a relevância fisiológica da RHBDD2 e a sua contribuição para a doença acima referida não é clara. Os dois maiores focos deste estudo foram (1) a investigação de como a mutação R85H na RHBDD2 contribui para a doença a nível celular; (2) investigar a função da RHBDD2 nativa a nível da célula e do organismo. Vários programas de alinhamento (ClustalO, muscle, tcoffee ferramentas de alinhamento) foram usados para prever a topologia da RHBDD2. Com base na comparação com outros membros da superfamília romboide, os domínios transmembranares (TMD) da RHBDD2 foram previstos. Após identificar cuidadosamente todos os aminoácidos chave nos alinhamentos, eu previ que a RHBDD2 é uma proteína transmembranar com 6 TMD, com ambas as terminações N- e C- presentes na face citoplasmática. Devido ao conhecimento prévio de que a RHBDD2 é expressa em níveis elevados na retina, consistente com a expressão a nível do mRNA na base de dados bioGPS, células de ratinho RPE foram selecionadas como materiais essenciais para as seguintes experiências. Plasmídeos para a sobre-expressão da RHBDD2 WT e da mutação R85H com uma tag de HA no C-terminal foram gerados no vector pLEX MCS, e usados para gerar células estáveis por transdução lentiviral. Estas linhas celulares juntamente com a linha celular com o vector vazio (EV) foram usadas para investigar a função da RHBDD2 a nível bioquímico e da biologia celular. Surpreendentemente, a expressão da forma mutante da RHBDD2 em células RPE, não induziu um stress constitutivo do retículo endoplasmático (RE). Não houce degradação da proteína mutante na linha celular R85H. Isto permitiu-me propor de forma preliminar, que o fenótipo da mutação R85H não é causado por stress do (RE). Por forma a analisar a localização do Et e mutante R85H, células RPE foram marcadas com um anticorpo anti-HA (para detectar RHBDD2 WT e mutante R85H) e com o marcador de stress do RE Calreticulin ou o marcador do cis-Golgi GM130. Imagens de imuno-fluorescência e co-localização mostraram que tanto a WT como a mutante RHBDD2 localizam predominantemente no aparelho de Golgi. Indicando que a mutante R85H localiza-se no mesmo compartimento celular que a proteína nativa, sugerindo que o fenótipo da doença não é causado por stress do RE ou por falta de folding da proteína. Ratinhos com deleção genética (KO) na RHBDD2 foram gerados pelo sistema CRISPR/Cas9 para definir a função da RHBDD2 e o fenótipo dos animais mutantes. Infelizmente, 5 animais fundadores morreram no primeiro dia de vida. Analise do tecido revelou que dois deles tinham INDELs e três deles eram nativos. Logo, a morte dos animais é pouco provável estar relacionada com o genótipo RHBDD2. A experiência foi repetida e 17 potencias fundadores foram obtidos. Resultados de sequênciaçao indicaram que dois animais fundadores tinham a mutação desejada, provavelmente como quimeras. Identificamos também animais fundadores com INDELs de 6 e 9 pares de bases. Experiências de imuno-precepitação acopladas a espectrometria de massa foram realizadas para identificar novos interactores da RHBDD2. Três IPs foram efetuados em diferentes condições (com ou sem crosslinker, acoplado com diferentes tampões de lavagem). Golgins (GM160, Golgin-84, Golgin-45), que atuam como plataformas de acoplamento membranar, foram co-immuno-precepitadas coma RHBDD2 em ambas as experiências com cross-linker, indicando que a RHBDD2 pode ter um papel na manutenção da estrutura do aparelho de Golgi. Componentes chaves das vesículas COPII foram também capturados nos IPs crosslinked e não crosslinked, indicando que a RHBDD2 pode contribuir para o transporte ER para Golgi dependente de COPII. Estes dois grupos apareceram tanto nos IPs da proteína RHBDD2 nativa como mutante R85H. De notar, o número total de identificações de peptídeos está diminuído em IPs de R85H em condições de crosslinking ou na sua ausência, indicando talvez que o mutante R85H, exibe uma alteração conformacional que resulta numa mudança de repertório de interactores. De acordo com as IPs, experiências de fragmentação do Golgi e da sua reorganização, foram efectuadas com RNA de interferência curto (siRNA) para fazer a atenuação da expressão (KD) de RHBDD2 em células HeLa. /2 horas após a transfecçao, as células foram tratadas com BFA, e depois a droga foi removida do meio. De seguida, as células foram fixadas a diferentes pontos de incubação. Esta experiência não resultou em nenhuma diferença óbvia, na cinética de da morfologia do aparelho de Golgi entre as células tratadas control ou com o KD, mas é importante notar que o siRNA não promove a atenuçao complete da expressão da RHBDD2, logo a presença de alguma RHBDD2 residual pode ter prevenido a observação de um fenótipo mais marcado. O papel fisiológico da RHBDD2 permanece desconhecido. Considerando os possíveis parceiros de ligação da RHBDD2 e a localização da RHBDD2, eu proponho que trabalho future se deva focar no teste do tráfego celular e da glicosilação em células sem expressão de RHBDD2 (KO). Também seria útil gerar um mutante R85H endógeno nas células RPE para avaliar a patologia da mutação, e claro, para avaliar o fenótipo dos ratinhos mutantes.
Schwartz, Daniel Hans [Verfasser]. "Development of an aqueous suspension of recombinant human bone morphogenetic protein-2 (rhBMP-2) = Entwicklung einer wässrigen Suspension des recombinanten menschlichen Knochenwachstumsproteins-2 (rhBMP-2) / vorgelegt von Daniel Hans Schwartz." 2005. http://d-nb.info/977660974/34.
Повний текст джерелаGrant, David William. "Reduced Burst Release of Bioactive rhBMP-2 from a Three-phase Composite Scaffold." Thesis, 2010. http://hdl.handle.net/1807/25605.
Повний текст джерелаLiebl, Oliver. "Knochenregeneration mit rhBMP-2 und unterschiedlichen Trägermaterialien - Biomechanische, fluoreszenzmorphometrische und radiomorphometrische Untersuchungen im Rattenmodell." Doctoral thesis, 2004. https://nbn-resolving.org/urn:nbn:de:bvb:20-opus-12011.
Повний текст джерелаThe aim of this study was to compare eight different materials on their applicability as carriers for rhBMP-2 (insoluble collagenous bone matrix ICBM, Hydroxyapatite, a-TCP, Algipore®, BioOss®, Bioglas, Collagen and Ethisorb®). In adult, full-grown Spargue-Dawley-rats critical-sized defects were created in the mandible (5mm) and the calvarial bone (7mm). In the femur continuity defects of 8 mm were created. The defects in the mandible and the calvarial bone have been filled with the current carriers enhanced by 10µg rhBMP-2. The continuity defects in the femur have been filled with the carriers combined with 25µg rhBMP-2. For synthetic HA, Algipore® and BioOss® no resorption could have been verified. a-TCP and Bioglas showed only marginal, ICBM and Ethisorb obvious biodegradation. All carriers enhanced by rhBMP-2 showed distinct new bone formation. The micro-radiological and morphometric analysis revealed the greatest and fastest increase of bone formation for ICBM. The calcium posphate ceramics showed only insignificant differences among themselves. Bioglas, Collagen and Ethisorb® showed the lowest amount of new bone formation. For all tested carriers implantation in the mandible showed the highest amount of bone induction. Explants of the femur were tested biomechanical. The highest Young´s modulus could have been observed for ICBM, a-TCP and HA. In this study ICBM qualified best as a carrier for recombinant human BMP-2. It has been extensively integrated in the new formed bone and showed excellent biodegradation
CHIN-LUNG, LIU. "Evalution of Synthetic Polymers-Gelatin Composite Scaffold As rhBMP-2 Delivery System in Bone Regeneration." 2006. http://www.cetd.com.tw/ec/thesisdetail.aspx?etdun=U0001-2407200619462700.
Повний текст джерелаWellmann, Mathias Windhagen Henning. "Der Effekt perkutaner rhBMP-2-Applikation während der Distraktionsosteogenese auf die trabekuläre Mikroarchitektur des Kallusregenerates /." 2005. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=014628430&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.
Повний текст джерела